Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| viridans streptococcal endocarditis: clinical, microbiological, and echocardiographic correlations. | infections caused by species within the viridans streptococci have been associated with different clinical characteristics. we studied 36 patients with viridans streptococcal endocarditis. complications were seen in 10 (32%) of 31 patients with native valve endocarditis and four (80%) of five with prosthetic valve endocarditis and included death in two, valve replacement in six, persistent infection in three, emboli in two, and congestive heart failure in nine. two-dimensional echocardiograms de ... | 1986 | 3745973 |
| xylitol metabolism in xylitol-sensitive and xylitol-resistant strains of streptococci. | the metabolism of xylitol in xylitol-sensitive strains (strains whose growth is inhibited by xylitol) and xylitol-resistant strains (growth not inhibited) of oral streptococci was compared. both xylitol-sensitive and xylitol-resistant strains took up xylitol. in the sensitive cells, the xylitol was probably transported via a phosphotransferase system. this resulted in intracellular accumulation of xylitol-5-phosphate and xylulose-5-phosphate. these metabolites were not detected in the xylitol-re ... | 1986 | 3751578 |
| possible pathogenic implications of right-sided polymicrobial endocarditis in a heroin abuser. | 1986 | 3758055 | |
| characterization of monoclonal antibodies specific for adhesion: isolation of an adhesin of streptococcus sanguis fw213. | monoclonal antibodies reactive to an adhesive strain of streptococcus sanguis (fw213) and nonreactive to a nonadhesive mutant (jl7) were derived from the fusion of myeloma line x63ag8.653 and spleen cells from balb/c mice immunized with live s. sanguis cells. five cell lines, belonging to subclasses of immunoglobulin g, produced monoclonal antibodies specifically directed against the adhesive strain. all five antibodies also failed to react with five additional, independently isolated, nonadhesi ... | 1986 | 3770949 |
| multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions. | a radioactivity-based assay was developed to define the participation of radioactively labeled cell types within the milieu of unlabeled partners in multigeneric aggregates. the cell types in these multigeneric aggregations consisted of various combinations of 21 strains representing five genera of human oral bacteria. the coaggregation properties of each cell type, when paired individually with various strains, were delineated and were unchanged when the microbes took part in the more complex m ... | 1986 | 3782029 |
| identification and preliminary characterization of a streptococcus sanguis fibrillar glycoprotein. | cell surface fibrils could be released from streptococcus sanguis 12 but not from strains 12na or n by freeze-thawing followed by brief homogenization. fibrils were isolated from the homogenate by ultracentrifugation or ammonium sulfate precipitation. electron microscopy demonstrated the presence of dense masses of aggregated fibrils in these preparations. under nondenaturing conditions, no proteins were seen in polyacrylamide gel electrophoresis (page). sodium dodecyl sulfate (sds)-page analysi ... | 1987 | 3793716 |
| right atrial vegetation in left ventricular-right atrial communication. | we report a case of right-sided endocarditis with left ventricular-right atrial communication in which right atrial vegetation was demonstrated by two-dimensional echocardiography. the present case demonstrates that the right atrial vegetation in ventricular septal defect is suggestive of left ventricular-right atrial communication. | 1987 | 3815918 |
| oxygen sensitivity of sugar metabolism and interconversion of pyruvate formate-lyase in intact cells of streptococcus mutans and streptococcus sanguis. | pyruvate formate-lyase (pfl) (formate acetyltransferase; ec 2.3.1.54) of oral streptococci is essential for metabolizing sugar into volatile compounds (formate, acetate, and ethanol). this enzyme is extremely sensitive to oxygen, and its activity is irreversibly inactivated by oxygen. when streptococcus sanguis was anaerobically starved, a part of the active form of pfl was converted into a reversible inactive form that was tolerant of oxygen. this reversible inactive enzyme could be reactivated ... | 1987 | 3818089 |
| a bacteriocin of actinobacillus actinomycetemcomitans. | an inhibitory factor from actinobacillus actinomycetemcomitans y4 was isolated, and its properties indicated that it was a bacteriocin (actinobacillicin). the bacteriocin was active against streptococcus sanguis strains, streptococcus uberis (fdc1), and actinomyces viscosus t14 as well as other strains of a. actinomycetemcomitans, but not against other crevicular bacteria, including other streptococci and actinomycetes. the activity of this bacteriocin was inhibited by pronase, trypsin, and heat ... | 1987 | 3818090 |
| purification and biochemical properties of a bacteriocin from actinobacillus actinomycetemcomitans. | extracts of certain strains of actinobacillus actinomycetemcomitans are inhibitory to strains of streptococcus sanguis such as s. sanguis atcc 10556. the isolation of a protein from an a. actinomycetemcomitans sonic extract which copurified with the inhibitory activity was accomplished by preparative isoelectric focusing, sephadex g-100 gel filtration chromatography, and preparative polyacrylamide gel electrophoresis (page). the resulting isolated protein, which focused at a ph of 6.1 to 6.3, ap ... | 1987 | 3818091 |
| penicillin therapy for treatment of experimental endocarditis caused by viridans streptococci in animals. | we studied the efficacy of penicillin and penicillin combined with streptomycin in the treatment of experimental endocarditis caused by viridans streptococci that are susceptible, tolerant, or relatively resistant to penicillin. rabbits with experimental endocarditis were treated with procaine penicillin (1.5 x 10(5) u/kg) administered twice daily or with procaine penicillin (1.5 x 10(5) u/kg) plus streptomycin (20 mg/kg) administered twice daily for five days. compared with control animals, ani ... | 1985 | 3846608 |
| effect of environmental conditions on the fluoride sensitivity of acid production by s. sanguis nctc 7865. | growth and environmental conditions affected the fluoride (f) sensitivity of acid production by streptococcus sanguis nctc 7865. cells grown glucose-limited in a chemostat were generally more sensitive than those harvested from cultures in which there was an excess of glucose (amino acid-limited). there was no consistent relationship between the growth rate of cells and their f sensitivity. slower-growing cells (mean generation time = 14 hr) were more sensitive than those growing quickly when gl ... | 1985 | 3855887 |
| additive inhibitory effects of combinations of fluoride and chlorhexidine on acid production by streptococcus mutans and streptococcus sanguis. | 1985 | 3856486 | |
| [biochemical capacity of glucan-producing cariogenic streptococci from human dental plaque with special reference to their acid-forming capacity]. | 1985 | 3856517 | |
| some factors affecting the diffusion of [14c]-lactate in human dental plaque. | the apparent diffusion rate, d, of lactate was significantly retarded in dental plaque fluid and a simulated plaque fluid consisting of a chemically-defined solution of salts, amino acids and albumin in phosphate buffer at ph 6.5. metabolic utilization of lactate in live plaque residue reduced d for lactate into such samples of residue, compared with killed samples. d in plaque residue was lower than in a previous study. increasing the packing density of killed plaque residue of streptococcus sa ... | 1985 | 3857030 |
| the relationship between the number of the bacterium streptococcus mutans at discrete sites on the dentition of macaque monkeys (macaca fascicularis) and the subsequent development of dental caries. | sixteen consecutively-born monkeys were used in a longitudinal study to determine the changes occurring in the total number of bacteria in the palatal grooves of the upper left first deciduous molar teeth, following the introduction of a sucrose-based diet. the total number of bacteria recovered from the grooves increased significantly following the diet change. twenty-nine days after the diet change, the number of streptococcus mutans had increased from a median value of less than 10(2) to appr ... | 1985 | 3857890 |
| inhibition of [3h]-thymidine uptake in human gingival fibroblasts by extracts from human dental plaque, oral bacteria of the streptococcus and actinomyces species. | extracts from human dental plaque, streptococcus mutans, streptococcus salivarius, streptococcus faecalis, streptococcus sanguis, actinomyces israelli and actinomyces odontolyticus inhibited [3h]-thymidine uptake by primary human gingival fibroblast cell lines as previous work has shown in respect of hela cells. the results show that a variety of oral bacteria, not usually considered to be periodontal pathogens, elaborate factors that adversely affect fibroblasts. | 1985 | 3857891 |
| infectivity and natural transmission of the bacterium streptococcus mutans in monkeys (macaca fascicularis) at different ages. | oral inoculation of adolescent monkeys with laboratory strains of streptococcus mutans resulted in only sporadic implantation. oral colonization of all strains, except possibly one, was not enhanced by dietary sucrose compared with glucose nor by precleaning of accessible tooth surfaces. infant animals were more susceptible to implantation by laboratory-maintained strains of strep. mutans than adolescent or adult animals. this difference was magnified when in-vivo maintained strep. mutans strain ... | 1985 | 3857904 |
| effect of oral nutrient limitation of gnotobiotic rats on acidogenic properties of dental plaque formed by oral streptococci. | the acid production rate of dental plaque, formed by different streptococci in gnotobiotic rats fed a high sucrose diet or starved for seven or eight days, was determined in vitro. starvation had no appreciable effect on acid production. this supports the idea that the small ph-lowering ability of plaque of caries-free or stomach tube-fed people in whom exposure of the teeth to fermentable carbohydrate is relatively low or negligible results from a change in the bacterial population rather than ... | 1985 | 3858302 |
| serum antibody responses to indigenous oral mucosal antigens and selected laboratory-maintained bacteria in recurrent aphthous ulceration. | sera from subjects with recurrent aphthous ulceration (rau) and control subjects were tested for relative levels of igm, igg, and iga antibodies against eight selected laboratory-maintained bacteria, including streptococcus sanguis which has been implicated in the etiopathogenesis of rau. there were no differences in relative serum antibody levels for any isotype against any bacteria between control and rau groups. rau subjects with active lesions were then paired with control subjects, and each ... | 1985 | 3859820 |
| the association between dietary sucrose consumption and microbial population shifts at six oral sites in man. | sucrose-related microbial population shifts were evaluated at 6 oral sites in 22 volunteers, who consumed high-sucrose diets for 21 days followed by low-sucrose diets for 21 days. culturing was performed at 0, 12, 21, 33 and 42 days of the 6-week experiment. over 50,000 microbial isolates were characterized and analysed. analysis of initial cultures showed the following site-specific microbial characteristics of the 6 sites evaluated: (1) molar fissures harboured higher levels of neisseria speci ... | 1985 | 3861144 |
| the surface free energy of oral streptococci after being coated with saliva and its relation to adhesion in the mouth. | contact angle measurements on layers of bacteria were used to determine the bacterial surface free energy (gamma b) of a variety of oral streptococcal strains, both without and after being coated with human whole saliva. at least four isolates of each species, either freshly isolated or laboratory strains, were used. the species streptococcus mutans, s. sanguis, and s. salivarius were homogeneous, having high surface free energies, and were not affected by saliva treatment (gamma b = 106 +/- 12 ... | 1985 | 3861650 |
| [antibacterial effect of calcium hydroxide on streptococcus sanguis as the root canal medicament]. | 1985 | 3862203 | |
| fatal septic shock after fine needle aspiration of a pancreatic pseudocyst. | fatal septic shock due to streptococcus sanguis developed eight days after fine needle aspiration (fna) of a pancreatic pseudocyst. the pseudocyst was adherent to the transverse colon (as is often the case with pseudocysts), and contamination of cyst fluid with colonic bacteria most likely initiated the infection. the patient had not received prophylactic antibiotics, even though the needle had been observed to pass through the bowel at the time of the cat-scan-guided aspiration. although the li ... | 1985 | 3863438 |
| bacteriological studies on endodontic paper points. | four brands of endodontic paper points were subjected to tests for sterility, antibacterial activity, and suitability as vehicles for bacteriological sampling procedures. no brand showed growth in the sterility assay. one brand showed weak but reproducible antibacterial activity. recovery of viable bacteria after absorption into the points varied and appeared to depend both on the brand of point and on the bacterial strain used. one brand appeared ineffectual for clinical bacteriological samplin ... | 1985 | 3863450 |
| effect of saliva substitutes upon binding of selected oral bacteria to hydroxyapatite. | 1985 | 3865705 | |
| anaerobic and aerobic metabolism of sorbitol in streptococcus sanguis and streptococcus mitior. | sorbitol-fermenting strains of streptococcus sanguis and streptococcus mitior were grown both anaerobically and in the presence of oxygen in a sorbitol-containing complex medium. washed-cell suspensions were incubated with an excess of sorbitol, and the production of lactate, formate, ethanol, and acetate was analyzed. moreover, we determined the lactate dehydrogenase and pyruvate formate-lyase activities in cell-free extracts of anaerobically grown cells. the anaerobically grown cells produced ... | 1985 | 3867686 |
| fluoride uptake and fluoride resistance in oral streptococci. | fluoride uptake was examined in the highly f-sensitive streptococcus salivarius strain 25975, the f-resistant mutant flr103, and the relatively insensitive s. sanguis h7pr3. f was taken up by all strains from media at neutral ph, containing 1 or 10 micrograms f/ml (0.053-0.526 mmol/l), and uptake was directly proportional to the delta ph that was maintained by the cells. the final intracellular concentrations of f in the f-resistant strains were higher than those in the f-sensitive strain. simil ... | 1985 | 3867687 |
| the relationships between streptococcal species and periodontopathic bacteria in human dental plaque. | the existence of antagonistic and commensal relationships between microorganisms was investigated. the predominant cultivable flora in 172 plaque samples from active and non-active sites in 32 human subjects with destructive periodontitis was determined. the presence of putative periodontopathic organisms (bacteroides gingivalis, bacteroides intermedius, bacteroides forsythus, wolinella recta, actinobacillus actinomycetemcomitans, and eikenella corrodens) in a site was correlated with the absenc ... | 1985 | 3868968 |
| bacterial profiles of subgingival plaques in periodontitis. | in this report over 400 subgingival plaque samples taken from over 110 patients were examined microscopically and culturally for 30 bacterial parameters. the patients could be placed into six disease categories based upon clinical criteria. the bacterial profile of each clinical category was generally distinctive of that category. periodontal patients who had been successfully treated and maintained had plaques that were populated by significantly higher proportions of streptococcus sanguis, act ... | 1985 | 3869648 |
| in vitro adherence of streptococcus sanguis atcc 10556 to various dental cements. | 1985 | 3880003 | |
| streptococcus-escherichia coli shuttle vector psa3 and its use in the cloning of streptococcal genes. | a shuttle vector that can replicate in both streptococcus spp. and escherichia coli has been constructed by joining the e. coli plasmid pacyc184 (chloramphenicol and tetracycline resistance) to the streptococcal plasmid pgb305 (erythromycin resistance). the resulting chimeric plasmid is designated psa3 (chloramphenicol, erythromycin, and tetracycline resistance) and has seven unique restriction sites: ecori, ecorv, bamhi, sali, xbai, nrui, and sphi. molecular cloning into the ecori or ecorv site ... | 1985 | 3883896 |
| coaggregation of oral bacteroides species with other bacteria: central role in coaggregation bridges and competitions. | seventy-three freshly isolated oral strains representing 10 bacteroides spp. were tested for their ability to coaggregate with other oral gram-negative and gram-positive bacteria. none coaggregated with any of the gram-negative strains tested, which included capnocytophaga gingivalis, c. ochracea, c. sputigena, and actinobacillus actinomycetemcomitans. strains of bacteroides buccae, b. melaninogenicus, b. oralis, and b. gingivalis failed to coaggregate with any of the gram-positive strains teste ... | 1985 | 3888842 |
| chemical properties and immunobiological activities of streptococcal lipoteichoic acids. | lipoteichoic acids (ltas) were chromatographically purified from crude phenol-water extract of whole cells of some streptococcal species, which included streptococcus pyogenes sv, streptococcus mutans 6715, and streptococcus sanguis atcc 10556. among these, special attention was paid to s. pyogenes lta for analyses of chemical composition and biological activities. all lta preparations contained equimolar amounts of glycerol and phosphorus. chemical analyses showed that s. pyogenes lta contained ... | 1985 | 3892980 |
| immunoelectrophoretic study of cell surface antigens from different streptococcus mutans serotypes and streptococcus sanguis. | antigens prepared from culture supernatants or whole cells of several cariogenic strains were examined by immunoelectrophoresis for their crossed antigenicity, with reference to streptococcus mutans omz175, serotype f. crossed immunoelectrophoresis revealed a crossreactivity between soluble extracellular and wall associated antigens of six strains of streptococcus mutans and one strain of streptococcus sanguis. protease destroyed the immunoreactivity of crossreactive antigens. one of them was sh ... | 1985 | 3894343 |
| antibiotic tolerance among clinical isolates of bacteria. | 1985 | 3895353 | |
| recovery of blood-borne bacteria from human urine. | recovery from the urine of organisms causing bacteraemia may depend on the bacterial species involved. the survival of the more common species of bacteria which cause bacteraemia was examined in human urine, serum and normal saline. all species survived well or grew in serum. haemophilus influenzae, streptococcus pneumoniae, streptococcus sanguis and group a streptococci were killed in all urine samples. the number of colony-forming units of staphylococcus aureus, staphylococcus epidermidis, sta ... | 1985 | 3897387 |
| bacteriology in juvenile periodontitis. a review. | 1985 | 3898064 | |
| expression in escherichia coli of streptococcal plasmid-determined erythromycin resistance directed by the cat gene promoter of pacyc 184. | the streptococcal erythromycin resistance (emr) plasmid psm7 (6.4 kb) and the e. coli vector pacyc184 (4.0 kb) were fused at their single ecori sites to form the bifunctional chimeric plasmid psm7184 (10.4 kb) in which the emr determinant was placed under control of the chloramphenicol acetyl transferase (cat) promoter of pacyc184. in the sense orientation (orientation i) of psm7, the cat promoter directed expression of emr in the e. coli host strains 294 and db11 more efficiently than did the i ... | 1985 | 3900334 |
| characterization of protein and mannan polysaccharide antigens of yeasts, moulds, and actinomycetes. | antigens in coccidioidin were compared with purified subfractions via tandem immunoelectrophoresis (iep) and by a combination of advancing line and crossed iep. rocket iep was suitable for titrating the reactions and showing the relationship between column fractions. these techniques required multicomponent antisera produced by hyperimmunization over many months and by the use of known standard migration pairs. the iep variations were used to chart the development of antisera against coccidioidi ... | 1985 | 3916766 |
| lactate dehydrogenase from streptococcus mutans: purification, characterization, and crossed antigenicity with lactate dehydrogenases from lactobacillus casei, actinomyces viscosus, and streptococcus sanguis. | a cytoplasmic fructose-1,6-diphosphate-dependent lactate dehydrogenase (ldh; ec 1.1.1.27) from streptococcus mutans omz175 was purified to homogeneity as judged by sodium dodecyl sulfate-gel electrophoresis. the purification consisted of ammonium sulfate precipitation of the cytoplasmic fraction, deae-sephacel and blue-sepharose cl.6b chromatography, and sephacryl s200 gel permeation. the catalytic activity of the purified enzyme required the presence of fructose-1,6-diphosphate with a broad opt ... | 1985 | 3917978 |
| transformation and fusion of streptococcus faecalis protoplasts. | nonconjugative plasmids were transferred by protoplast fusion among streptococcus faecalis strains and from streptococcus sanguis to s. faecalis. s. faecalis protoplasts were also transformed with several different plasmids, including the tn917 delivery vehicle ptv1. transformation was reproducible, but low in frequency (10(-6) transformants per viable protoplast). a new shuttle vector (pam610), able to replicate in escherichia coli and s. faecalis, was constructed and transformed into s. faecal ... | 1985 | 3920209 |
| leukocyte migration inhibition in recurrent aphthous ulceration. | leukocyte inhibitory factor (lif) production in response to streptococcal antigens and oral mucosa was examined as a possible pathogenic mechanism in recurrent aphthous ulceration (rau). mononuclear cells from rau patients and controls were stimulated with antigens prepared from two species of streptococci and from oral mucosa. candida albicans and foreskin were tested as control antigens. there were some statistically significant differences in leukocyte migration inhibition between patients an ... | 1985 | 3920363 |
| in-vitro inhibition of mycobacteria by viridans streptococci. | eleven strains of mycobacterium tuberculosis and 13 other species of mycobacteria were examined for susceptibility to 26 strains of viridans streptococci. susceptibility was detected by the simultaneous antagonism technique, but not by tests of deferred antagonism. it is concluded that in-vitro inhibition of mycobacterial growth by actively growing streptococci is due to a peroxide-mediated antagonism which is too variable to be of value for identification. however, a standard bactericidal test ... | 1985 | 3920400 |
| bactericidal activity of human lysozyme, muramidase-inactive lysozyme, and cationic polypeptides against streptococcus sanguis and streptococcus faecalis: inhibition by chitin oligosaccharides. | the basis of the bactericidal activity of human lysozyme against streptococcus sanguis was studied. experiments were designed to evaluate the role of lysozyme muramidase activity in its bactericidal potency. inactivation of the muramidase activity of lysozyme was achieved by reduction of essential disulfides with dithiothreitol (dtt) or by incubation with the chitin oligosaccharides chitotriose and chitobiose. muramidase-inactive lysozyme, prepared by reduction with dtt, was equal in bactericida ... | 1985 | 3922894 |
| characterization and molecular cloning of cryptic plasmids isolated from lactobacillus casei. | four small cryptic plasmids were isolated from lactobacillus casei strains, and restriction endonuclease maps of these plasmids were constructed. three of the small plasmids (plz18c, plz19e, and plz19f1; 6.4, 4.9, and 4.8 kilobase pairs, respectively) were cloned into escherichia coli k-12 by using pbr322, pacyc184, and puc8 as vectors. two of the plasmids, plz18c and plz19e, were also cloned into streptococcus sanguis by using pva1 as the vector. hybridization by using nick-translated cloned 32 ... | 1985 | 3923929 |
| comparison of single doses of amoxicillin or of amoxicillin-gentamicin for the prevention of endocarditis caused by streptococcus faecalis and by viridans streptococci. | recent recommendations for the prophylaxis of endocarditis in humans have advocated single doses or short courses of antibiotic combinations (beta-lactam plus aminoglycoside) for susceptible patients in whom enterococcal bacteremia might develop or for patients at especially high risk of developing endocarditis (e.g., patients with prosthetic cardiac valves). we tested the prophylactic efficacy (in rats with catheter-induced aortic vegetations) of single doses of amoxicillin plus gentamicin agai ... | 1985 | 3925031 |
| antistreptococcal activity of sch 34343, a new penem antibiotic. | the in-vitro activity of sch 34343 was compared with that of penicillin and cefotaxime against clinical isolates of streptococci. sch 34343 was highly active against streptococcus pneumoniae, viridans streptococci, streptococci of groups a, b, c and g, and str. bovis. ninety per cent of these strains were inhibited by less than or equal to 0.25, less than or equal to 0.12 and less than or equal to 0.06 mg/l of sch 34343, penicillin and cefotaxime respectively. activity of sch 34343 against str. ... | 1985 | 3928578 |
| lymphocyte function in recurrent aphthous ulceration. | lymphocyte blast transformation responses to mitogens and to streptococcal, adenovirus type 1, and oral mucosal antigens were studied in 32 patients with recurrent aphthous ulceration (rau) and matched control subjects. rau patients showed significantly depressed (p less than 0.05) responses to mitogens in comparison with matched controls, but there was no significant difference between patients and controls in response to any of the antigens tested. these results indicate that neither cell-medi ... | 1985 | 3930669 |
| genetic transformation in streptococcus sanguis. identification, surface spreading and competence of invasive strains of streptococcus sanguis lancefield groups h and w and other invasive viridans streptococci. | of 46 viridans streptococci isolated from cases of probably true bacteremia, 26 strains or 56% could be grouped serologically in the lancefield system. eight strains (17%) were identified as serogroup h (characteristic of streptococcus sanguis), ten strains (22%) as serogroup w (a new group antigen found in s. sanguis) and eight strains (17%) as serogroup k (related to s. mitis (mitior)). the high number of serogroupable viridans streptococci was specially due to the introduction of serogroup w ... | 1985 | 3931412 |
| chemical and immunological characterization of a novel amphipathic antigen from biotype b streptococcus sanguis. | a new type of amphipathic antigen was extracted from whole cells of streptococcus sanguis atcc 10557 (biotype b, serotype ii) by the phenol/water method. the extract was treated with nuclease p1, and was applied to a column of sepharose 6b. each fraction was checked by passive haemagglutination (pha) and immunodiffusion tests against anti-10557 serum which was obtained by immunizing rabbits with whole cells of strain atcc 10557. strong pha activity was demonstrated in the first hexose-containing ... | 1985 | 3932593 |
| n-methyl transferase of streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin b antibiotics: amino acid sequence and its homology to cognate r-factor enzymes from pathogenic bacilli and cocci. | the nucleotide sequence of a structural gene erme for ribosomal rna (rrna) n6-amino adenine n-methyl transferase (nmt) of streptomyces erythraeus, cloned by thompson et al. [gene 20 (1982) 51-62], has been determined. the nmt amino acid (aa) sequence deduced from the nucleotide sequence contains extensive homology to aa sequences of cognate nmts specified by: (1) plasmid pe194 from staphylococcus aureus, 30% g + c, ermc; (2) plasmid pam77 from streptococcus sanguis, 43% g + c; as well as to (3) ... | 1985 | 3934045 |
| salivary immunoglobulin a and serum antibodies to streptococcus mutans ribosomal preparations in dental caries-free and caries-susceptible human subjects. | caries-free subjects or individuals with low caries susceptibility exhibited significantly higher (p less than 0.001) levels of naturally occurring salivary immunoglobulin a (iga) and serum igg, iga, and igm antibodies to a streptococcus mutans ribosomal preparation than subjects with high caries susceptibility. absorption of saliva and serum samples with s. mutans ribosomal preparations, but not with other s. mutans antigens or with escherichia coli and neisseria gonorrhoeae ribosomal preparati ... | 1986 | 3941001 |
| enzyme-linked immunosorbent assay for the detection of antibodies to nutritionally variant streptococci in patients with endocarditis. | the viridans streptococci are responsible for 50%-55% of microbial endocarditis. among these varied species, the nutritionally variant streptococci (nvs) have recently been associated with culture-negative endocarditis and are responsible for 5%-10% of all streptococcal endocarditis. the isolation and identification of these bacteria has been hampered by the extremely fastidious nature of their growth requirements as well as by their slow growth rate. in addition, their antibiotic sensitivity va ... | 1986 | 3941275 |
| streptococcal endocarditis: single vs. combination antibiotic therapy and role of various species. | forty-eight patients with 51 episodes of infective endocarditis caused by streptococci were studied in a comparison of the efficacy of single antibiotic therapy versus that of combination therapy. there was no statistical difference in terms of clinical course, toxicity, and mortality between the group given a single drug and that given combination therapy. however, one patient experienced two relapses, in both instances after high-dose penicillin therapy. the species and antibiotic susceptibili ... | 1986 | 3952425 |
| active streptokinase from the cloned gene in streptococcus sanguis is without the carboxyl-terminal 32 residues. | the streptokinase expressed by the cloned gene in streptococcus sanguis has a molecular weight of about 44 000 [malke, h., gerlach, d., kohler, w., & ferretti, j.j. (1984) mgg, mol. gen. genet. 196, 360-365] while the molecular weight of the native streptokinase is 47 000. the structural and activity differences of the cloned streptokinase (csk) as expressed by s. sanguis and the native streptokinase (nsk) were investigated. from a partially purified csk, two active fractions were obtained by re ... | 1986 | 3954985 |
| alterations due to ampicillin and rifampicin in s. sanguis and s. aureus isolated from dental plaque. an electron microscopic study. | in this work we analysed the ultrastructural changes in s. aureus and s. sanguis after exposure to rifampicin and ampicillin respectively. s. aureus grown in the presence of rifampicin showed ultrastructural changes that can be summarized as follows: the peripheral cell-wall increased to three to five times the normal thickness; the cross-walls were considerably (two to ten times) thicker than normal; after 8 h of exposure a complete lysis occurred. s. sanguis grown in the presence of ampicillin ... | 1986 | 3955780 |
| effects of oxygen on pyruvate formate-lyase in situ and sugar metabolism of streptococcus mutans and streptococcus sanguis. | the strictly anaerobic metabolism of sugar in strains of streptococcus mutans and streptococcus sanguis was studied because deep layers of dental plaque are strictly anaerobic. galactose-grown cells of these streptococcal strains had higher pyruvate formate-lyase activity than did glucose-grown cells. among these strains, two strains of s. mutans had a significantly higher pyruvate formate-lyase activity than did the others. this enzyme is extremely sensitive to oxygen, and even in situ the enzy ... | 1985 | 3965391 |
| surface structures (peritrichous fibrils and tufts of fibrils) found on streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera. | we screened 36 strains of streptococcus sanguis biotype i and 8 strains of s. sanguis biotype ii for the presence of surface structures and for their ability to coaggregate with actinomyces viscosus, actinomyces naeslundii, and fusobacterium nucleatum. negative staining under an electron microscope revealed detectable surface structures on all s. sanguis strains. the majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. they usually a ... | 1985 | 3965396 |
| effects of exopolysaccharide production by viridans streptococci on penicillin therapy of experimental endocarditis. | the influence of exopolysaccharide production by viridans streptococci on penicillin therapy of endocarditis was studied in rabbits with experimental aortic-valve endocarditis. animals were first infected with penicillin-susceptible streptococci known to produce varying amounts of exopolysaccharide in cardiac vegetations. treatment with procaine penicillin, 300,000 u every 12 hr, was begun one to two days after infection. after five days of therapy, animals infected with streptococcus sanguis ii ... | 1985 | 3965587 |
| recombination-deficient streptococcus sanguis. | a uv-sensitive derivative was obtained from streptococcus sanguis challis. the organism could be transformed with a number of small streptococcal plasmids at frequencies equal to, or 1 logarithm below, the transformation frequencies for the parent organism. however, transformation with chromosomal dna was greatly impaired in the uv-sensitive derivative. | 1985 | 3988349 |
| serological identification of streptococcus sanguis and str mitior. | a total of 165 strains of streptococcus sanguis and str mitior were selected on the basis of their biochemical reactions using established identification procedures. these strains were also classified using api database and were then screened against five candidate grouping sera. biochemical tests and serological identification were in general complementary, but no regular associations between biotype and serological reaction were observed. | 1985 | 3988957 |
| phosphatidyl glycerolphosphate serves as glycerolphosphate donor in polymer synthesis. | phosphatidyl glycerolphosphate was found to serve as the glycerolphosphate donor for polymer synthesis. when cdp-diglyceride and radiolabeled glycerolphosphate were incubated with the membrane enzyme prepared from streptococcus sanguis, active syntheses of radiolabeled lipids and polymers were observed. the synthesis of polymer was not inhibited by low concentration of unlabeled phosphatidylglycerol. when [3h, 32p]glycerolphosphate was used, the polymer synthesized contained both 3h and 32p. the ... | 1985 | 3994726 |
| 'hot' and 'cold' lesions detected by gallium-67 scintigraphy in a pyogenic liver abscess. | most pyogenic liver abscesses appear to be 'not' in a 67ga-citrate study; rarely, a 'warm' or 'cold' area may also be seen. we present a patient with a pyogenic liver abscess that had both 'cold' and 'hot' areas in the 67ga-citrate study. this striking finding is probably related to the different stages of evolution of the abscess. | 1985 | 3996439 |
| cloned gtfa gene of streptococcus mutans lm7 alters glucan synthesis in streptococcus sanguis. | streptococcus mutans lm7 (bratthall serotype e) chromosomal dna was partially digested with ecori and ligated into the positive-selection plasmid vector pop203(a2+). the ligation mixture was transformed into escherichia coli, and transformants were selected for tetracycline resistance. recombinant-bearing clones were screened for their ability to ferment raffinose, using the procedure of robeson et al. (j. bacteriol. 153:211-221, 1983). one raffinose-fermenting clone was isolated and found to co ... | 1985 | 3997243 |
| adhesion of streptococcus sanguis ch3 to polymers with different surface free energies. | the adhesion of the oral bacterium streptococcus sanguis ch3 to various polymeric surfaces with surface free energies (gamma s) ranging from 22 to 141 erg cm-2 was investigated. suspensions containing nine different bacterial concentrations (2.5 x 10(7) to 2.5 x 10(9) cells per ml) were used. after adhesion for 1 h at 21 degrees c and a standardized rinsing procedure, the number of attached bacteria per square centimeter (nb) was determined by scanning electron microscopy. the highest number of ... | 1985 | 4004241 |
| isolation of actinomyces bacteriophage from human dental plaque. | human dental plaque samples were screened for the presence of bacteriophage for actinomyces viscosus and streptococcus sanguis. none of the 336 samples yielded phage for s. sanguis, but 10 contained virulent actinomyces phage. a high host cell specificity was observed in that one phage isolate infected only a. viscosus t14v, eight phage isolates infected only a. viscosus mg-1, and one infected both strains. none was capable of productively infecting various other actinomyces strains that represe ... | 1985 | 4008044 |
| resistance in oral streptococci after repeated three-dose erythromycin prophylaxis. | ten healthy volunteers, from whom no erythromycin-resistant oral streptococci could be isolated initially, received three doses of erythromycin stearate (1.0, 0.5 and 0.5 g) on two separate occasions with a one-week interval between them. after the second administration all volunteers yielded moderately resistant strains (mic 1-4 mg/l) and four harboured highly resistant streptococci (mic 16- greater than 256 mg; mbc 128- greater than 256 mg/l). erythromycin-resistant strains persisted in eight ... | 1985 | 4008379 |
| extracellular matrix proteins (fibronectin, laminin, and type iv collagen) bind and aggregate bacteria. | the normal microbial colonization of sites in the body's tissues by certain bacteria requires that the bacteria first bind to extracellular secreted constituents, cell-surface membranes, or cell matrixes. this study examines two interactions of a variety of bacteria with the cell matrix noncollagenous proteins fibronectin and laminin and with basement membrane (type iv) collagen. adherence of bacteria to matrix proteins coated on tissue culture wells was examined with the use of radiolabeled bac ... | 1985 | 4014440 |
| [spondylodiscitis after embolization of an extramedullary intraspinal arteriovenous fistula]. | the authors report a case of spondylitis th11-th12 occurred 1 month after embolization of an intraspinal extramedullary arteriovenous fistulae; this fistulae was fed by 11th left intercostal artery. the infecting organism isolated from the affected intervertebral disc was streptococcus sanguis a common agent of dental abscess. | 1985 | 4020738 |
| indifferent streptococci in normal and purulent eyes of neonates. | the incidence of indifferent streptococci in the eyes of neonates less than 6 days old was investigated. the isolation of indifferent streptococci was significantly higher in infants with sticky eyes. speciation using a shortened identification scheme was carried out on isolates; streptococcus mitior and streptococcus sanguis were the most common species in both purulent and non-infected eyes. streptococcus mutans, a species not normally found in edentulous infants, comprised 14% of indifferent ... | 1985 | 4031105 |
| enhanced killing of penicillin-treated gram-positive cocci by human granulocytes: role of bacterial autolysins, catalase, and granulocyte oxidative pathways. | staphylococci pretreated with subminimal inhibitory concentrations (submic) of cell-wall active antibiotics exhibit increased susceptibility to killing by human polymorphonuclear leukocytes (pmns), even when phagosome information is impaired by the mold metabolite, cytochalasin b. to investigate the role of specific bacterial factors in the process, studies were carried out with organisms lacking catalase (streptococci) or cell-wall autolytic enzymes and compared to findings with staphylococcus ... | 1985 | 4036175 |
| cell surface components of streptococcus sanguis: relationship to aggregation, adherence, and hydrophobicity. | cell surfaces of aggregation, adherence, and hydrophilic variants of streptococcus sanguis were compared with cell surfaces of the parent strain with regard to their protein and antigenic constituents. cell surface molecules were released by digestion with mutanolysin. extraction with sodium dodecyl sulfate (sds) urea, lithium diiodosalicylate, and boiling water did not solubilize any material which stained with agno3 in an sds-polyacrylamide gel electrophoresis gel. the parent organism s. sangu ... | 1985 | 4044521 |
| genetic transformation in streptococcus sanguis. effects on genetic transformation by culture filtrates of streptococcus sanguis (serogroups h and w) and streptococcus mitis (mitior) with reference to identification. | streptococcus sanguis (serogroups h and w) strains are frequently competent in genetic transformation. in a collection of 18 strains of s. sanguis isolated from blood cultures, 12 strains were spontaneously competent in transformation by streptomycin-resistant dna, and three strains were induced to competence by the use of culture filtrates containing competence factor (cf). culture filtrates from spontaneously competent s. sanguis strains only induced competence in transformation of strains of ... | 1985 | 4050439 |
| regulation of glycolytic rate in streptococcus sanguis grown under glucose-limited and glucose-excess conditions in a chemostat. | the biochemical mechanisms of the acidogenic potential of streptococcus sanguis atcc 10556 grown in glucose-excess and glucose-limited continuous culture were studied. the rate of acid production during the glucose metabolism by the cells grown under glucose limitation (glucose-limited cells) was 2.1 to 2.6 times that by the cells grown in an excess of glucose (glucose-excess cells). when the glucose-limited cells were metabolizing glucose, intracellular concentrations of glucose 6-phosphate, fr ... | 1985 | 4055023 |
| comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of streptococcus sanguis g9b. | streptococcus sanguis g9b was grown in continuous culture at different generation times and ph values in media containing either glucose or fructose and differing in the concentrations of na+ and k+. the growth ph, carbohydrate, and cation concentration each affected the yield of organisms, their ability to adhere to saliva-coated hydroxyapatite beads, and their hydrophobicity, as measured by adhesion to hexadecane. there was no correlation between adhesion to saliva-coated hydroxyapatite beads ... | 1985 | 4055033 |
| albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles. | a procedure involving blocking with bovine albumin was useful for differentiating between streptococcal interactions with components of experimental salivary pellicles similar to those which form on teeth and potential interactions with uncoated areas of mineral. | 1985 | 4055036 |
| characterization of nutritionally variant streptococci by biochemical tests and penicillin-binding proteins. | a comparative study of 60 strains of nutritionally variant streptococci (nvs) with 34 strains of streptococcus mitis and 37 strains of streptococcus sanguis ii showed the presence of a red chromophore which was absent in the other streptococcal species. by using the conventional microbiological tests, only small differences were found between the nvs and the two other related species. in contrast a clear-cut delineation was found by the api 20 strep system of identification. all nvs contained py ... | 1985 | 4066913 |
| bacterial meningitis following pantopaque myelography. a case report and literature review. | a case of acute bacterial meningitis following pantopaque myelography is reported, and the literature reviewed pertaining to this uncommon but potentially fatal complication. a positive gram's stain is most helpful in differentiating bacterial from chemical meningitis. treatment of the meningitis before and after determination of its cause is described. preventive steps include removal of pantopaque from the subarachnoid space immediately at the conclusion of fluoroscopy, and observance of stric ... | 1985 | 4094954 |
| myocardial depression and nephrotic syndrome in streptococcus sanguis endocarditis. | nephrotic syndrome is a most unusual complication of infective endocarditis. we report a case of streptococcus sanguis endocarditis of the tricuspid valve in a patient with a small congenital ventricular septal defect who developed this complication. severe myocardial depression also occurred and the clinical behaviour and subsequent recovery of this closely paralleled that of the nephrotic syndrome. these unusual associations, their possible interactions and pathogeneses are discussed. | 1985 | 4095256 |
| relation of macromolecular synthesis in streptococci to efficiency of transformation by markers of homospecific and heterospecific origin. | in previous studies with streptococcus sanguis and s. pneumoniae as recipients and donors of transforming deoxyribonucleic acid (dna), it was found that heating recipients just prior to exposure to dna caused an increase in the number of transformants induced by heterospecific dna relative to that induced by homospecific dna. in the present studies, s. sanguis recipients were found to recover from this effect of heat (48 c, 15 min) when incubated at 37 c before exposure to dna. inhibitors of nuc ... | 1974 | 4130687 |
| [occurrence of neuraminidase and acylneuraminate pyruvate-lyase in streptococcus sanguis]. | 1972 | 4146470 | |
| serology of streptococcus sanguis: localization of antigens with unlabeled antisera. | a new technique for the electron microscope localization of bacterial surface antigens, using unlabeled antisera, has been developed. this technique, called the "immuno-coating reaction" was applied to serological studies of streptococcus sanguis, using antisera which had been shown to be specific for this species by gel diffusion analysis. the results of the immuno-coating reaction confirmed the specificity of the antisera for s. sanguis. in addition, the data indicate that some of the antigens ... | 1973 | 4199721 |
| lipoteichoic acids from streptococcus sanguis. | two lipoteichoic acids, membrane (mlta) and wall (wlta), have been purified from streptococcus sanguis by sepharose and ecteola-cellulose column chromatographies and concanavalin a-conjugated sepharose affinity column chromatography. the teichoic acids were homogenous as judged by disc gel electrophoresis, column chromatography, and double diffusion tests. both mlta and wlta consisted of glycerol, phosphate, glucose, and fatty acids in the ratios of 0.95:1:0.71:0.046 and 0.99:1:0.79:0.023, respe ... | 1974 | 4208135 |
| glycerolphosphate-containing cell wall polysaccharides from streptococcus sanguis. | six glycerolphosphate-containing tetraheteroglycans, a, b-1, b-2, b-3, b-4, and b-5, have been purified from the formamide extracts of streptococcus sanguis by alcohol and acetone precipitations, sephadex g-75, and diethylaminoethyl-cellulose column chromatography. the polysaccharides were judged as at least 95% pure by analytical disc gel electrophoresis and immune double diffusion against rabbit antiserum. they were shown to be cell wall polysaccharides, since they formed a single band of iden ... | 1974 | 4218231 |
| inhibition of enzymes by human salivary immunoglobulin a. | human whole saliva inhibited bacterial neuraminidases and the inhibition was found to reside in the salivary iga fraction. further, salivary immunoglobulin (ig)a inhibited various bacterial enzymes and toxins: neuraminidases from streptococcus mitis, streptococcus sanguis, and clostridium perfringens, hyaluronidase and chondroitin sulfatase from oral bacteria, diphtheria toxin, and streptolysin o. the inhibitory activity of salivary iga did not correlate with that of serum on the basis of minimu ... | 1973 | 4354148 |
| inhibition of transformation by sodium metaperiodate in streptococcus sanguis (wicky): demonstration that deoxyribonucleic acid binding sites contain amino acids. | addition of sodium metaperiodate to competent streptococcus sanguis (wicky) cells at 0 or 37 c revealed that the deoxyribonucleic acid binding sites consist of amino acids. | 1974 | 4364331 |
| turnover of phosphatidylglycerol in streptococcus sanguis. | 1974 | 4370528 | |
| calcification of selected strains of streptococcus mutans and streptococcus sanguis. | nine strains of cariogenic streptococcus mutans and two strains of streptococcus sanguis were tested for their ability to form hydroxyapatite. the cells were examined by x-ray diffraction and electron microscopy for apatite crystals after growth in a synthetic calcification medium. each of the test isolates, except for one strain of s. sanguis, produced intracellular mineral. two strains of s. mutans formed both intra- and extracellular crystals. there was no apparent relationship between calcif ... | 1974 | 4371107 |
| differential susceptibility of human iga immunoglobulins to streptococcal iga protease. | iga protease, a proteolytic enzyme found in human saliva and colonic fluid, hydrolyzes human serum iga immunoglobulins to yield fab(alpha) and fc(alpha) fragments. the enzyme is produced by organisms in the normal human microflora and can be purified from culture filtrates of the common human oral organism streptococcus sanguis (american type culture collection no. 10556). iga protease is inactive against all other protein substrates examined including the other classes of human immunoglobulins. ... | 1974 | 4436434 |
| the occurrence of streptococcus mutans and streptococcus sanguis in the blood of endocarditis patients. | 1972 | 4502189 | |
| establishment of defined microbial ecosystems in germ-free rats. i. the effect of the interactions of streptococcus mutans or streptococcus sanguis with veillonella alcalescens on plaque formation and caries activity. | 1972 | 4504260 | |
| nutritional requirements of streptococcus sanguis. | 1972 | 4506985 | |
| [interaction of soluble extracellular polysaccharides and concanavalin a produced by streptococcus mutans and streptococcus sanguis]. | 1972 | 4513399 | |
| production of bactericidal concentrations of hydrogen peroxide by streptococcus sanguis. | 1973 | 4515970 | |
| invertase activity in streptococcus mutans and streptococcus sanguis. | 1973 | 4516065 | |
| recovery of streptococcus mutans and streptococcus sanguis from a dental explorer after clinical examination of single human teeth. | 1973 | 4516070 | |
| regulation of dextransucrase formation by streptococcus sanguis. | 1973 | 4518082 | |
| the effect of topical acidulated fluoride on percentage of streptococcus mutans and streptococcus sanguis in interproximal plaque samples. | 1973 | 4518084 | |
| the localization and retention of 32p and 45ca within surface deposits of streptococcus sanguis and the influence of such deposits on the release of these isotopes from enamel. | 1973 | 4518166 |