Publications

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suppression of carotenoid synthesis and its effect on the activity of photosynthetic bacterial chromatophores. 195813504141
cytochrome reactions in chromatium. 195813535717
photophosphorylation by isolated chromatophores of the purple sulfur bacteria. 195813560025
thiorhodaceae. i. the effects of sodium thioglycolate on the photosynthetic and dark metabolism of purple sulphur bacteria. 195813573226
[utilization of organic compounds by photosynthetizing purple sulfur bacteria from beloe lake]. 195813632412
the demonstration of photochemical reducing sites in chromatium sp. 195913672214
photoproduction of hydrogen, photofixation of nitrogen and a unified concept of photosynthesis. 196113684408
on the prosthetic group of an rhp-type haem protein from chromatium. 196113687280
relation of photosynthetic activity to carotenoid-bacteriochlorophyll interaction in chromatium. 196113702555
carbon metabolism in chromatium. 196113702556
cytochromes in chlorophyll-containing particles of chromatium and chlorobium thiosulfatophiulm. 196013716746
light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. 196113731247
[enriched culture for various purple sulfur bacteria]. 196113747780
separation of two light reactions in noncyclic photo-phosphorylation of green plants. 196113763582
isolation and properties of two soluble heme proteins in extracts of the photoanaerobe chromatium. 196013797318
influence of carotenoids on the infra-red spectrum of bacteriochlorophyll in chromatium. 195913799328
studies on bacterial chromatophores. i. reversible disturbance of transfer of electronic excitation energy between bacteriochlorophyll-types in chromatium. 196013804436
studies on the natural state of bacteriochlorophyll. 195913837984
the amino acid composition of the soluble chromatium haem proteins. 196113865520
enzymes of the reductive pentose phosphate cycle in the purple and in the green photosynthetic sulphur bacteria. 196213914044
[on carbon metabolism by the purple sulfur bacterium chromatium vinosum]. 196213940555
[on relations between the physiological state and the average length of fluorescence of bacteriochlorophyll in the cells of photosynthesizing bacteria]. 196313982983
[atmospheric nitrogen fixation by purple sulfur bacteria during growth on mineral and organic media]. 196214002446
studies on bacterial chromatophores. ii. energy transfer and photooxidative bleaching of bacteriochlorophyll in relation to structure in normal and carotenoid-depleted chromatium. 196314015480
evidence for the photochemical reduction on coenzyme q in chromatophores of photosynthetic bacteria. 196214021651
[carotenoids in thiorhodaceae. i. okenoneas the principal carotenoid in chromatium okenii perty]. 196314044829
[carotenoids in thiorhodaceae. ii. carotenoid composition of thiospirillum jenense winogradsky and chromatium vinosum winogradsky]. 196314044830
[carotenoids in thiorhodaceae. iii. carotenoids in chromatium warmingii migula]. 196314044831
[studies on the status and conversion of pigments in purple and green photosynthetizing bacteria]. 196314052869
cytochrome-chlorophyll interaction: the nature of the dark reaction. 196314087332
ribulose diphosphate carboxylase in thiorhodaceae. 196314114934
photoreduction of ubiquinone and photooxidiation of phenazine methosulfate by chromatophores of photosynthetic bacteria and bacteriochlorophyll. 196414128127
microbiological fractionation of sulphur isotopes. 196414135528
light-induced oxidation of cytochromes in photosynthetic bacteria between 20 and-170 degrees. 196414179445
absorption changes in bacterial chromatophores.the magnitude and kinetics of photo-induced absorption changes in bacterial chromatophores (r. rubrum, r. spheroides and chromatium) have been studied as a function of potential, established by added redox couples. no photochanges can be observed above +0.55 v or below -0.15 v. the loss of signal at the higher potential is centered at +0.439 v and follows a one-electron change. the loss of signal at the lower potential is centered at -0.044 v and is also consistent with a one-electron change. bo ...196414185583
the pyruvate phosphoroclastic reaction and light-dependent nitrogen fixation in bacterial photosynthesis. 196414212555
role of ferredoxin in the reductive assimilation of co2 and acetate by extracts of the photosynthetic bacterium, chromatium. 196414212563
sulphur metabolism in thiorhodaceae. i. quantitative measurements on growing cells of chromatium okenii. 196414218435
ubiquinone-40 and vitamin k-2 (40) in chromatium vinosum. 196414240125
studies on photophosphorylation. xii. noncyclic photophosphorylation in chromatophores of photosynthetic bacteria. 196414243612
bacterial ferredoxin. 196414244728
photoreduction of nicotinamide-adenine dinucleotide by a cell-free system from chromatium. 196414249088
studies on the electron-transfer systems in photosynthetic bacteria. iv. kinetics of light-induced cytochrome reactions and analysis of electron-transfer paths. 196414249834
an attempt at quantitation of the sharp light-induced electron paramagnetic resonance signal in photosynthetic materials. 196414249836
characterization of poly-beta-hydroxybutyrate extracted from different bacteria.lundgren, d. g. (syracuse university, syracuse, n.y.), r. alper, c. schnaitman and r. h. marchessault. characterization of poly-beta-hydroxybutyrate extracted from different bacteria. j. bacteriol. 89:245-251. 1965.-poly-beta-hydroxybutyrate (phb) from different bacterial genera was studied with regard to its crystal structure, infrared absorption, intrinsic viscosity, and electron microscopy. all phb samples precipitated from dilute chloroform solution gave identical x-ray diffractograms confir ...196514255672
[study of the carotenoids of purple sulfur bacteria]. 196414267381
sulphur metabolism in thiorhodaceae. ii. stoichiometric relationship of co2 fixation to oxidation of hydrogen sulphide and intracellular sulphur in chromatium okenii. 196414274131
[co2 fixation and intermediate metabolism in chromatium okenii perty]. 196414310165
immunochemical studies on the photoactive subcellular particles from chromatium. 195914426842
oxidation-reduction reactions in the photosynthetic bacterium chromatium. i. absorption spectrum changes in whole cells. 196014428812
oxidation-reduction reactions in the photosynthetic bacterium chromatium. ii. dependence of light reactions on intensity of irradiation and quantum efficiency of cytochrome oxidation. 196014428813
spectrofluorometry of pyridine nucleotide reactions in chromatium. 195914428814
[morphology of sulfur purple bacteria from the family chromatium in relation to the composition of the culture medium]. 195914432366
[the consumption by chromatium vinosum of acetic and propionic acids simultaneously present in the culture medium]. 196014445551
[the participation of propionic acid in the constructive metabolism of chromatium vinosum]. 196014445552
[use of acetic acid as a sole source of carbon in photosynthetizing bacteria chromatium vinosum]. 196014446163
fractionation of isotopes in relation to the problem of elemental sulphur transport by micro-organisms. 196214453750
[formation of chlorophyll by purple and green bacteria during their photoautotrophic and photoheterotrophic development]. 196214476474
[observations on swarming of chromatium okenii]. 196214486136
[uptake substances of chromatium okenii]. 196214498518
effect of duckweed cover on greenhouse gas emissions and odour release from waste stabilisation ponds.treatment of wastewater in stabilisation pond systems prevents the negative environmental impact of uncontrolled disposal of sewage. however, even a natural treatment system may generate secondary negative environmental impacts in terms of energy consumption, emission of greenhouse gases and emission of odorous compounds. whereas natural systems have an advantage over electro-mechanical systems in that they use less hardware and less energy, it is not yet known whether secondary environmental ef ...200314510229
investigation of the reduced high-potential iron-sulfur protein from chromatium vinosum and relevant model compounds: a unified picture of the electronic structure of [fe(4)s(4)](2+) systems through magnetic and optical studies.magnetization measurements and variable temperature optical spectroscopy have been used to investigate, within the 4-300 k temperature range, the electronic structure of the reduced high-potential iron protein (hipip) from chromatium vinosum and the model compounds (cat)(2)[fe(4)s(4)(sr)(4)], where rs(-) = 2,4,6-triisopropylphenylthiolate (1), 2,6-diphenylphenylthiolate (2), diphenylmethylthiolate (3), 2,4,6-triisopropylbenzylthiolate (4, 4'), 2,4,6-triphenylbenzylthiolate (5, 5'), 2,4,6-tri-ter ...200314552635
selective staining of proteins with hydrophobic surface sites on a native electrophoretic gel.chemical proteomics aims to characterize all of the proteins in the proteome with respect to their function, which is associated with their interaction with other molecules. we propose the identification of a subproteomic library of expressed proteins whose native structures are typified by the presence of hydrophobic surface sites, which are often involved in interactions with small molecules, membrane lipids, and other proteins, pertaining to their functions. we demonstrate that soluble globul ...200314582643
a simple method for making multiple tests of a micro-organism. 195214927559
phototrophic purple bacterium chromatium minutissimum does not synthesize cytokinins under optimal growth conditions. 200315255204
successful recombinant production of allochromatium vinosum cytochrome c' requires coexpression of cmm genes in heme-rich escherichia coli jcb712.cytochrome c' from the purple photosynthetic bacterium allochromatium vinosum (ccp) displays a unique, reversible dimer-to-monomer transition upon binding of no, co, and cn(-). this small, four helix bundle protein represents an attractive model for the study of other heme protein biosensors, provided a recombinant expression system is available. here we report the development of an efficient expression system for ccp that makes use of a maltose binding protein fusion strategy to enhance peripla ...200515649399
[ecophysiological properties of photosynthesizing bacteria from the black sea chemocline zone].in may 1998, during the fifty-first voyage on board the research vessel professor vodyanitskii, a comparative study was conducted of the species diversity of green and purple sulfur bacteria in the water column of the chemocline zone at deep-sea stations and on the bottom surface of the black sea shallow regions. at three deep-sea stations, the accumulation of photosynthesizing bacteria in the chemocline zone at a depth of 85-115 m was revealed on the basis of the distribution of potential value ...200515938401
long-term population dynamics of phototrophic sulfur bacteria in the chemocline of lake cadagno, switzerland.population analyses in water samples obtained from the chemocline of crenogenic, meromictic lake cadagno, switzerland, in october for the years 1994 to 2003 were studied using in situ hybridization with specific probes. during this 10-year period, large shifts in abundance between purple and green sulfur bacteria and among different populations were obtained. purple sulfur bacteria were the numerically most prominent phototrophic sulfur bacteria in samples obtained from 1994 to 2001, when they r ...200516000760
reducing red color intensity of seafood wastewater in facultative pond.studies were carried out on the growth of chromatium sp. on seafood wastewater (sfww), which under facultative conditions and light exposure produced red pigment. the strain grew and utilized organic matter in both dark and light exposure conditions, but it produced red pigment when exposed to light. the growth was repressed by aerobic condition. the red color intensity was reduced by about 32.5+/-1.5 and 70.8+/-2.8% when kept under dark and static conditions, or aerobic and light exposure condi ...200616154743
transcription of three sets of genes coding for the core light-harvesting proteins in the purple sulfur bacterium, allochromatium vinosum.the nucleotide sequence of the puf operon coding for the subunits of the photosynthetic reaction center and the core light-harvesting complex (lh1) of the purple sulfur bacterium, allochromatium (a.) vinosum (formally chromatium vinosum), was completely determined. unlike other known puf operons, which contain only one set of genes coding for the lh1 apoproteins, pufb and pufa, the a. vinosum puf operon included three sets of pufb and pufa genes with a gene order of pufb (1) a (1) lmcb (2) a (2) ...200216245138
nitrogen fixation by photosynthetic bacteria in lowland rice culture.propanil (3',4'-dichloropropionanilide) was a potent inhibitor of the nitrogenase activity of blue-green algae (cyanobacteria) in flooded soil, but the herbicide at comparable concentrations was not toxic to rice, protozoa, and nitrogen-fixing bacteria. ethanol-amended flooded soils treated with propanil exhibited higher rates of nitrogenase activity than those not treated with the herbicide. the enhanced nitrogenase activity in propanil-treated soils was associated with a rise in the population ...198016345507
factors determining annual changes in bacterial photosynthetic pigments in holomictic lake cisó, spain.the pigments and biomass of anoxygenic phototrophic bacteria were measured during a year cycle in lake cisó (girona, spain). two genera, chromatium and chlorobium, accounted for most of the bacterial population. the bacteria were present throughout the year despite complete mixing of the lake during fall and winter. this was possible because the sulfide production in the sediment was high enough to make the lake anaerobic to the very surface. solar radiation, temperature, and biomass of chromati ...198316346436
change in size of chromatium minus cells in relation to growth rate, sulfur content, and photosynthetic activity: a comparison of pure cultures and field populations.the size frequency distribution of planktonic cells of purple sulfur phototrophic bacteria was measured at several depths in a bacterial layer of lake cisó (spain). the bacterioplankton was dominated by chromatium minus (87 to 94% of the total biomass). the largest cells of c. minus were found in the top part of the bacterial layer. in addition, the in situ and potential specific photosynthetic activity (co(2) fixation and acetate uptake) and specific pigment content were measured in relation to ...198716347330
populations of anaerobic phototrophic bacteria in a spartina alterniflora salt marsh.habitat-simulating media were used with the hungate anaerobic roll tube technique to enumerate culturable anaerobic photosynthetic bacteria in sediment, tidal waters, and spartina alterniflora plant samples collected from the salt marsh at sapelo island, ga. no phototrophs were detected in samples of creekside (low marsh) sediment or in tidal waters in creekside regions. in the high marsh region, 90% of anaerobic phototrophic bacteria occurred in the top 5 mm of sediment and none were detected b ...198816347646
effects of grazing by the free-living soil amoebae acanthamoeba castellanii, acanthamoeba polyphaga, and hartmannella vermiformis on various bacteria.cultures of 10 different bacteria were used to serve as food sources for axenically grown acanthamoeba castellanii, acanthamoeba polyphaga, and hartmannella vermiformis. the nonpigmented enterobacteriaceae escherichia coli k-12 and klebsiella aerogenes appeared to be excellent feed to all three amoebae. hardly any growth or ammonium production was observed in tests with chromatium vinosum and serratia marcescens, which share the presence of pigmented compounds. distinct differences in net ammoni ...199316349000
carotenoid-bacteriochlorophyll energy transfer in lh2 complexes studied with 10-fs time resolution.in this report, we present a study of carotenoid-bacteriochlorophyll energy transfer processes in two peripheral light-harvesting complexes (known as lh2) from purple bacteria. we use transient absorption spectroscopy with approximately 10 fs temporal resolution, which is necessary to observe the very fast energy relaxation processes. by comparing excited-state dynamics of the carotenoids in organic solvents and inside the lh2 complexes, it has been possible to directly evaluate their energy tra ...200616428274
photohydrogen production in chromatium. 195216561820
on the mechanism of chlorophyll-cytochrome interaction: the temperature insensitivity of light-induced cytochrome oxidation in chromatium. 196016590592
electron transfer between biological molecules by thermally activated tunneling.a theory of electron transfer between two fixed sites by tunneling is developed. vibronic coupling in the individual molecules produces an activation energy to transfer at high temperatures, and temperature-independent tunneling (when energetically allowed) at low temperature. the model is compared with known results on electron transfer in chromatium and in rhodopseudomonas spheroides. it quantitatively interprets these results, with parameters whose scale is verified by comparison with optical ...197416592178
tunneling in chromatium chromatophores: detection of a hopfield charge-transfer band.we have observed a weak charge-transfer band in the cytochrome c-p(870) electron-transfer reaction in chromatium vinosum chromatophores at 10 k and at 85 k. first, the intermediate acceptor, i, was trapped in the reduced state by lowering the redox potential at room temperature, then illuminating with white light at low temperature for 20 min. next, illumination by broadband infrared (1-3 mum, 6.5 kw/m(2)) for 4 hr at 10 k decreased the i(-) electron spin resonance signal by 30%. one-hour infrar ...198416593405
enzyme activities of the carbon reduction cycle in some photosynthetic organisms.profile analyses of the enzymes comprising the photosynthetic carbon reduction cycle have been performed in extracts of dark grown and greening euglena gracilis var. bacillaris. chlorella pyrenoidosa grown photoautotrophically, in the light with glucose or in the dark with glucose, tolypothrix tenuis, chromatium and leaves of spinach. amounts of activity are compared with the level of photosynthetic co(2) fixation. only in chromatium were all enzyme activities sufficient to support the in vivo r ...196916657059
biocarbonate effect on the photophosphorylation catalyzed by chromatophores isolated from chromatium strain d: xii. structure and function of chloroplast proteins.photophosphorylation catalyzed by chromatophores prepared from chromatium strain d was stimulated by bicarbonate. the stimulative effect was ph dependent and the lower the ph the more marked the activation. at ph 8.0, bicarbonate (8 mm) exhibited a negligible effect, whereas at ph 7.0 approximately a 5-fold activation was observed. the apparent activation constant of bicarbonate was determined to be approximately 10.2 mm, at which concentration approximately a 7-fold activation of photophosphory ...197216658218
isotope fractionation in photosynthetic bacteria during carbon dioxide assimilation.the delta (pdb) (13)c values have been determined for the cellular constituents and metabolic intermediates of autotrophically grown chromatium vinosum. the isotopic composition of the hco(3) (-) in the medium and the carbon isotopic composition of the bacterial cells change with the growth of the culture. the delta (pdb) (13)c value of the hco(3) (-) in the media changes from an initial value of -6.6 per thousand to +8.1 per thousand after 10 days of bacterial growth and the delta (pdb) (13)c v ...197516659105
microencapsulation of chloroplast particles.chloroplast and photosystem i particles were encapsulated in small spheres (about 20 mum diameter) with an artificial membrane built up by cross-linking amino groups of protamine with toluenediisocyanate. the artificial membrane was permeable to small substrate and product molecules but not to soluble proteins. photosystem i activity was retained by the encapsulated chloroplast particles. washed photosystem i particles were encapsulated with the soluble proteins, ferredoxin, and ferredoxin-nadp ...197616659564
photooxidative damage in photosynthetic activities of chromatium vinosum.the capacity of photosynthetic co(2) fixation in the anaerobic purple-sulfur bacterium, chromatium vinosum is markedly impaired by strong illumination (9 x 10(4) lux) in the presence of 100% o(2). in the absence of hco(3) (-), decline in activity occurred gradually, with about 40% of the initial activity remaining after a 1-hour incubation. the addition of 50 millimolar hco(3) (-) to the incubation medium resulted in a measurable delay (about 30 minutes) of the inactivation process. ribulose-1,5 ...197816660651
effect of betaine on enzyme activity and subunit interaction of ribulose-1,5-bisphosphate carboxylase/oxygenase from aphanothece halophytica.the presence of betaine, a quaternary ammonium compound, at a concentration (0.5 molar) reported to accumulate inside aphanothece halophytica in response to increasing external salinity, slightly promoted ribulose-1,5-bisphosphate (rubp) carboxylase activity. kcl at 0.25 molar inhibited rubp carboxylase about 55%. betaine relieved the inhibition by 0.25 m kcl and the original uninhibited activity was restored at 1 m betaine. other osmoregulatory solutes such as sucrose and glycerol also reduced ...198616664941
[ni(xbsms)ru(co)2cl2]: a bioinspired nickel-ruthenium functional model of [nife] hydrogenase.as a model of the active site of [nife] hydrogenases, a dinuclear nickel-ruthenium complex [ni(xbsms)ru(co)2cl2] was synthesized and fully characterized. the three-dimensional structure reveals a nickel center in a square-planar dithioether-dithiolate environment connected to a ruthenium moiety via a ni(mu-sr)2ru bridge. this complex catalyzes hydrogen evolution by electroreduction of the weakly acidic et3nh+ ions in n,n-dimethylformamide and is therefore the first functional bioinspired model o ...200616711679
dynamics of electron transfer from high-potential cytochrome c to bacteriochlorophyll dimer in photosynthetic reaction centers as probed using laser-induced temperature jump.laser-induced temperature jump experiments were used for testing the rates of thermoinduced conformational transitions of reaction center (rc) complexes in chromatophores of chromatium minutissimum. the thermoinduced transition of the macromolecular rc complex to a state providing effective electron transport from the multiheme cytochrome c to the photoactive bacteriochlorophyll dimer within the temperature range 220-280 k accounts for tens of seconds with activation energy 0.166 ev/molecule. th ...200717262223
utilization of solid "elemental" sulfur by the phototrophic purple sulfur bacterium allochromatium vinosum: a sulfur k-edge x-ray absorption spectroscopy study.the purple sulfur bacterium allochromatium vinosum can use elemental sulfur as an electron donor for anoxygenic photosynthesis. the elemental sulfur is taken up, transformed into intracellular sulfur globules and oxidized to sulfate. commercially available "elemental" sulfur usually consists of the two species cyclo-octasulfur and polymeric sulfur. the authors investigated whether only one sulfur species is used or at least preferred when alc. vinosum takes up elemental sulfur and forms globules ...200717379736
[purple sulfur bacteria isolated from reservoirs of the yavoriv sulfur deposit].three pure cultures of purple sulfur bacteria were isolated from reservoirs of the yavoriv sulfur deposit. the studying of their morphology, cytology and physiology has confirmed the belonging of these bacteria to chromatiaceae family and has allowed identifying them as thiocapsa sp., lamprocystis sp. and chromatium sp.200717427405
quantum efficiency of cytochrome oxidation in a photosynthetic bacterium.illumination of chromatium with light absorbed by bacteriochlorophyll causes the oxidation of intracellular cytochrome. the quantum efficiency of this reaction approaches one electron per photon at 589 millimicrons and at wavelengths between 862 and 908 millimicrons. the efficiency of converting excitation energy to chemical energy is estimated to be about 30 percent.196217793874
understanding nicotinamide dinucleotide cofactor and substrate specificity in class i flavoprotein disulfide oxidoreductases: crystallographic analysis of a glutathione amide reductase.glutathione reductase (gr) plays a vital role in maintaining the antioxidant levels of the cytoplasm by catalyzing the reduction of glutathione disulfide to reduced glutathione, thereby using nadph and flavin adenine dinucleotide as cofactors. chromatiaceae have evolved an unusual homolog that prefers both a modified substrate (glutathione amide disulfide [gassag]) and a different cofactor (nadh). herein, we present the crystal structure of the chromatium gracile glutathione amide reductase (gar ...200717977556
granulosicoccaceae fam. nov., to include granulosicoccus antarcticus gen. nov., sp. nov., a non-phototrophic, obligately aerobic chemoheterotroph in the order chromatiales, isolated from antarctic seawater.a gram-negative, motile by tuft flagella, obligately aerobic chemoorganoheterotrophic, sphere-form bacterium, designated imcc3135(t), was isolated from the antarctic surface seawater of king george island, west antarctica. the strain was mesophilic, neutrophilic, and requiring nacl for growth, but neither halophilic nor halotolerant. the 16s rrna gene sequence analysis indicated that the strain was most closely related to genera of the order chromatiales in the class gammaproteobacteria. the mos ...200718062226
light-induced generation of electric potential difference in membranes of purple and green sulfur bacteria.when associated with a planar phospholipid membrane, chromatophores isolated from photosynthetic sulfur bacteria chromatium minutissimum, ectothiorhodospira shaposhnikovii, and chlorobium limicola f. thiosulfatophilum were shown to generate a light-induced transmembrane electric potential difference measured by a direct method using macroelectrodes and a voltmeter. the maximal photoelectric responses were observed upon the addition of 1,4-naphthoquinone in combination with phenazine methosulfate ...197718265514
chromatium species: an emerging bioindicator of crude oil pollution of tidal mud flats in the niger delta mangrove ecosystem, nigeria.establishing microbiological indices for the monitoring of environmental decay by crude oil pollution in the niger delta region has been a major concern of our current researches. chromatium species, a purple, gram positive pleomorphic, motile, microaerophlic sulfur bacterium offers a good potential for use in the assessment of the short term effects of oil pollution of tidal mud flats in the niger delta mangrove ecosystem. its response to the november 22, 2003 spillage at the qua iboe estuary a ...200918600468
epigenetics in acute myeloid leukemia.acute myeloid leukemia (aml) is a disease characterized by uncontrolled proliferation of clonal neoplastic hematopoietic precursor cells. this leads to the disruption of normal hematopoiesis and bone marrow failure. major breakthroughs in the past have contributed to our understanding of the genetic failures and the changed biology in aml cells that underlie the initiation and progression of the disease. it is now recognized that not only genetic but also epigenetic alterations are similarly imp ...200818692688
thiophaeococcus mangrovi gen. nov., sp. nov., a photosynthetic, marine gammaproteobacterium isolated from the bhitarkanika mangrove forest of india.a coccoid, phototrophic purple sulfur bacterium was isolated in pure culture from a mud sample collected from brackish water in the bhitarkanika mangrove forest of orissa, india, in a medium containing 2 % nacl (w/v). this bacterium, strain ja304(t), was gram-negative and had a requirement for nacl. intracellular photosynthetic membranes were of the vesicular type. the colour of the phototrophically grown culture was saddle-brown. bacteriochlorophyll a and the carotenoid lycopene were present as ...200818984710
a single-cell view on the ecophysiology of anaerobic phototrophic bacteria.quantitative information on the ecophysiology of individual microorganisms is generally limited because it is difficult to assign specific metabolic activities to identified single cells. here, we develop and apply a method, halogen in situ hybridization-secondary ion mass spectroscopy (hish-sims), and show that it allows simultaneous phylogenetic identification and quantitation of metabolic activities of single microbial cells in the environment. using hish-sims, individual cells of the anaerob ...200819004766
co-occurrence of denitrification and nitrogen fixation in a meromictic lake, lake cadagno (switzerland).the nitrogen cycling of lake cadagno was investigated by using a combination of biogeochemical and molecular ecological techniques. in the upper oxic freshwater zone inorganic nitrogen concentrations were low (up to approximately 3.4 microm nitrate at the base of the oxic zone), while in the lower anoxic zone there were high concentrations of ammonium (up to 40 microm). between these zones, a narrow zone was characterized by no measurable inorganic nitrogen, but high microbial biomass (up to 4 x ...200919397681
unexpected extracellular and intracellular sulfur species during growth of allochromatium vinosum with reduced sulfur compounds.before its uptake and oxidation by purple sulfur bacteria, elemental sulfur probably first has to be mobilized. to obtain more insight into this mobilization process in the phototrophic purple sulfur bacterium allochromatium vinosum, we used hplc analysis and x-ray absorption near-edge structure (xanes) spectroscopy for the detection and identification of sulfur compounds in culture supernatants and bacterial cells. we intended to identify soluble sulfur compounds that specifically occur during ...200919423634
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