Publications

[hpl serum levels in intrauterine retardation of fetal growth during late pregnancy (author's transl)].		2016	4848903
radiological case of the month.		2015	4854015
score changes following repeated administration of mental status questionnaires.		2011	4854204
experimental blackout and the visual system.		2016	4860699
thiogalactoside transacetylase. amino- and carboxyl-terminal studies.		1967	4863046
[autoimmune hepatitis].		2016	4865241
assessment of behavior characteristics in mental retardates.		2016	4867070
bacteroides vulgatus, a nonsporeforming anaerobe for testing fluid thioglycollate medium.		1968	4874465
preliminary survey of medical care in hawaii. 1. hospital care.		2016	4876386
[traffic facial injuries].		2016	4884373
[unitary discharge of pontine reticular formation and phasic ponto-geniculo-occipital activity in the cat under reserpine].		1969	4895788
motor proficiency and other variables in educable mentally retarded children.		2016	4898365
history of the wisconsin maternal mortality study and survey committee.		2016	4905769
therapeutic effectiveness of amantadine hydrochloride in influenza a2--double blind studies.		1968	4906644
a cellular deficit in the reconstitutive capacity of immune populations of lymphoid cells demonstrable in studies of delayed hypersensitivity in mice. evidence for thymus-bone marrow cell synergism.	a cell-transfer system was employed in the present work to investigate several characteristics of the capacity of immune and normal lymphoid cells to transfer the delayed response to methylated human serum albumin in lethally irradiated syngeneic recipients. spleen cells derived from donor mice immunized with goose erythrocytes were far less effective in transferring responsiveness when compared with equal numbers of normal cells. statistical analyses indicated a frequency of 1 reactive cell or ...	1971	4928817
dna synthesis in cell-free extracts of a dna polymerase-defective mutant.		1970	4933688
[clinical use of prostaglandins type e(pge) and type a(pga)].		2016	4939298
an improved method for chromosome preparation from mouse liver.		1971	4945732
[incorporation into bacillus subtilis ribosomes of ribonucleic acid synthesized under the influence of chloramphenicol in the presence of actinomycin d].		1965	4958012
[on comparative characteristics of the brain stem arteries in man and monkey (cercopithecus sabaecus l.)].		1965	4959240
observations on visna virus-infected cell cultures stained with acridine orange.		1966	4959372
[hemodynamic effects of nitroglycerin in dogs].		1968	4970730
failure of antibody formation after massive exposure to the rh (d) antigen.		2016	4977102
[studies on the influence of bacteria and intestinal helminths of host, taking in consideration germ-free animals. ii. studies on the influence of bacteria on the development of helminths in germ-free animals].		1969	4978124
leucocyte groups in baboons tested with human antisera.		1970	4987704
[organ and species specificity of leucine aminopeptidase from bovine lens].		1970	4991388
[long-term therapy of the chronic bronchitis syndrome using tanderil].		2014	4992075
[role of the parietal associative cortex in the conditioned-reflex activity of rhesus monkeys].		1971	5004228
the location of the fluid pump in the cornea.	1. fluid transport across rabbit corneal tissue has been investigated by observing the movement of fluid interfaces under the microscope, or by mounting the tissue between two chambers and observing the displacement of menisci within capillary tubes.2. in both cases, the endothelial layer supported on a thin sheet of connective tissue is capable of pumping fluid in a direction out of the cornea, against a head of pressure. the volume of fluid moved may amount to twelve times the thickness of the ...	1972	5016991
excretion of steroid hormone metabolites in bile of male rats.		1972	5028879
[blood gases and acid-base equilibrium in children with bronchial asthma].		2014	5029483
hepatic protein and nucleic acid content in dipsosurus dorsalis following hypophysectomy and treatment with estradiol-17 and growth hormone.		2016	5029486
inflammatory bowel disease in ankylosing spondylitis.		2016	5030136
local and systemic effects of phenylalanine on intestinal transport of tyrosine and tryptophan.		1972	5037606
[graphic models of the flow of information in the health care of prague university students].		2016	5045146
[diagnosis and treatment of conductive hearing losses in childhood].		2012	5050011
[stereochemistry of ephedrine-like compounds. absolute configuration of the four isomeric cis- and trans-6-amino-6,7,8,9-tetrahydro-5h-benzocycloheptenols-(5)].		2016	5053529
a new species of chigger, blankaartia pauli (acarina: trombiculidae), from the southeastern united states.		1972	5057499
[x-ray radioisotope diagnosis of tumor metastases of urogenital organs into the skeleton].		2015	5063119
[pathogenesis and prevention of diabetes mellitus and its complications].		2016	5069982
drug screening for endocrine effects: influence of body weight on organ weight.		1972	5070844
conduction velocities of lemniscal and thalamocortical fibers: their somatotopic differentiation and a rule of connection in the thalamic relay system.		1972	5075713
[isoagglutinin level in human saliva].		1972	5076480
[trial in abdominal surgery of a new molecule, trimebutine, in resumption of postoperative transit].		2016	5088602
the direct immunofluorescence in the presumptive identification of bacteroides species.	two kits containing fluorescein labeled-polyvalent antisera to bacteroides fragilis, bacteroides distasonis, bacteroides ovatus, bacteroides thetaiotaomicron, bacteroides vulgatus (fluoretec-f) and to bacteroides melaninogenicus subsp. melaninogenicus and intermedius and to bacteroides asaccharolyticus (non-oral strains) (fluoretec-m) were evaluated for the identification of the respective organisms in bacterial cultures and clinical specimens. in both cases immunofluorescent tests were compared ...	1982	6126973
specificity of immunoglobulin m antibodies in normal human serum that participate in opsonophagocytosis and intracellular killing of bacteroides fragilis and bacteroides thetaiotaomicron by by human polymorphonuclear leukocytes.	studies were performed to determine the specificity of immunoglobulin m (igm) antibodies in normal human serum that participate in opsonophagocytosis and intracellular killing of bacteroides fragilis 1365 and bacteroides thetaiotaomicron 1343 by human polymorphonuclear leukocytes. purified normal human igm was adsorbed with washed heat-killed cells of the homologous strains and heterologous strains of b. fragilis, b. thetaiotaomicron, bacteroides vulgatus, bacteroides distasonis, and bacteroides ...	1980	6160104
lymphocytotoxic and microbial antibodies in crohn's disease and matched controls.	patients with crohn's disease and age, sex and seasonally matched healthy controls were studied for cold lymphocytotoxic and a variety of microbial antibodies. lymphocytotoxic antibody titers were increased significantly in the crohn's patients compared to controls, but did not correlate with any of the microbial titers. antibodies to a pseudomonas-like bacterium and bacteroides vulgatus were also increased in the patients, but titers to chlamydia trachomatis, rotavirus and norwalk virus were no ...	1981	6275790
in vitro activity of mk0787 (n-formimidoyl thienamycin) and other beta-lactam compounds against bacteroides spp.	the susceptibilities of 82 strains of the bacteroides fragilis group to eight beta-lactam compounds, lincomycin, and metronidazole were determined by using an agar dilution technique. mk0787 (n-formimidoyl thienamycin) was the most active compound, inhibiting all strains at a concentration of 1 microgram/ml. metronidazole was the only other drug of similar activity. of the beta-lactam compounds, cefoxitin and mk0787 showed uniform activity against all species, whereas most other compounds were r ...	1981	6282191
characterization of cephalosporinases from bacteroides fragilis, bacteroides thetaiotaomicron and bacteroides vulgatus.	the susceptibility of 80 bacteroides fragilis group strains isolated from clinical specimens to beta-lactam antibiotics was investigated by agar dilution method. twenty strains showed high resistance to the antibiotics. the resistance level of the isolates to cephaloridine was related to the amount of beta-lactamase activity (cephalosporinase; csase) produced. b. fragilis gn-11477, b. thetaiotaomicron gn11478 and b. vulgatus gn11479 were selected from among the csase producing strains, and the e ...	1983	6332105
open study of ceftazidime in serious infections due to multiply-resistant bacteria.	we treated 37 infections in 32 patients who had one or more significant underlying diseases. most of the responsible micro-organisms were multiresistant, but were initially sensitive to ceftazidime. they included 16 pseudomonas spp., 10 serratia spp., 6 klebsiella spp., 7 other enterobacteriaceae, 3 staphylococcus spp., 3 strict anaerobes and 1 acinetobacter anitratus. indications for ceftazidime encompassed a broad spectrum of diseases: 10 complicated uti, 13 skeletal and soft tissue infections ...	1983	6352619
rapid glutamic acid decarboxylase test for identification of bacteroides and clostridium spp.	a rapid 4-h test for glutamic acid decarboxylase is described for the identification of certain anaerobic bacteria. the test substrate consisted of 1.0 g of l-glutamic acid, 0.3 ml of triton x-155, and 0.05 g of bromcresol green sodium salt in 1 liter of water. the substrate was dispensed in 0.5-ml amounts into test tubes, and a turbid suspension was made with the test organism. the test was then incubated aerobically at 35 degrees c for 4 h. the development of a blue color was considered positi ...	1984	6376535
alpha-ketoglutarate metabolism by cytochrome-containing anaerobes.	during growth in the presence of tracer amounts of exogenously supplied alpha-keto[1-14c]glutarate (akg) or alpha-keto [5-14c]glutarate, cytochrome-containing bacteroides fragilis strain 2044 and bacteroides vulgatus strain 8482 incorporated extremely small amounts of radioactivity into cell macromolecules and protoheme. under identical conditions, bacteroides "l" strain 7cm and bacteroides buccae strain j1 incorporated substantial label from [5-14c]akg, but not [1-14c]akg, into cellular macromo ...	1983	6413047
distribution and effects of a defined six-member murine-derived microflora in gnotobiotic gerbils.	the gnotobiotic gerbil was selected as a model with which to study the effects of colonization with a defined microflora on organ morphology, histology, and selected blood biochemical parameters. gerbils were maintained germfree for 13 months but failed to reproduce, presumably because of the enlarged cecum. a colony of gnotobiotic gerbils that was associated with a bacterial flora consisting of lactobacillus brevis, streptococcus faecalis, staphylococcus epidermidis, bacteroides vulgatus, enter ...	1984	6426388
adoptive transfer of immune enhancement of experimental ulcerative colitis.	previous experiments with the carrageenan model for ulcerative colitis have shown that the inflammatory response in guinea pigs can be enhanced by immunization with and subsequent feeding of bacteroides vulgatus to experimental animals. the present studies showed that only certain strains of b. vulgatus are capable of provoking immune enhancement of ulcerative colitis. animals were fed carrageenan and various strains of viable b. vulgatus after immunization with a strain of b. vulgatus isolated ...	1984	6480115
the intestinal microflora of infants: composition of fecal flora in breast-fed and bottle-fed infants.	the fecal flora of 35 breast-fed and 35 bottle-fed babies was determined. bifidobacteria were the predominant fecal bacteria in both groups. conversely, the counts of most of the other bacteria, such as bacteroides, eubacteria, peptococci, veillonella, clostridia, enterobacteria, streptococci, and bacilli in the bottle-fed group were significantly higher than those in the breast-fed group. the frequencies of occurrence of lecithinase positive clostridia, clostridia-others, pseudomonas and bacill ...	1984	6513816
quantitative variability in requirements for opsonization of strains within the bacteroides fragilis group.	requirements for opsonization of various strains within the species bacteroides fragilis, bacteroides thetaiotaomicron, bacteroides vulgatus, and bacteroides distasonis were investigated. the minimal concentration of normal human serum that facilitated maximal uptake by human peripheral leukocytes of 14c-labeled strains ranged from 5% to 80%. heated (56 c at 30 min) serum produced less uptake than untreated serum, suggesting that complement was required for opsonization. using human serum deplet ...	1983	6631060
enhancement of experimental ulcerative colitis by immunization with bacteroides vulgatus.	previous studies with the guinea pig model for ulcerative colitis have shown that the inducing agent, carrageenan, does not provoke ulcerations in germfree guinea pigs, but animals monoassociated with bacteroides vulgatus develop cecal ulcerations when fed carrageenan. in an effort to define the role of b. vulgatus in this model system, conventional guinea pigs were immunized with b. vulgatus before carrageenan treatment. immunized animals developed both circulating antibody and positive skin te ...	1983	6642651
infectivity of organisms recovered from polymicrobial abscesses.	the ability to cause subcutaneous abscesses in mice was used to identify the pathogens among the bacteria recovered from 13 clinical abscesses. a total of 35 isolates (30 anaerobes and 5 aerobes), 16 of which were encapsulated, were recovered from these abscesses. encapsulated organisms included eight bacteroides spp. (three bacteroides asaccharolyticus and one strain each of bacteroides oralis, bacteroides intermedius, bacteroides biacutus, bacteroides vulgatus, and bacteroides ruminicola subsp ...	1983	6642674
activation of hageman factor by lipopolysaccharides of bacteroides fragilis, bacteroides vulgatus, and fusobacterium mortiferum.	preincubation of lipopolysaccharides (lps) of bacteroides fragilis, bacteroides vulgatus, and fusobacterium mortiferum with purified human hageman factor (hf) followed by addition of purified human prekallikrein resulted in the activation of hf, as measured by the generation of kallikrein using the tripeptide substrate n-alpha-benzoyl-l-proline-l-phenylalanine-l-arginine-4-nitroanilide. addition of purified hf, which had been preincubated with the lps, to hf-deficient plasma resulted in the acce ...	1984	6718941
susceptibility of intestinal anaerobes to new beta-lactam antibiotics.	in vitro susceptibility of 473 isolates of anaerobic bacteria derived from patients hospitalized for treatment of abdominal trauma was tested against third-generation cephalosporins--moxalactam, cefotaxime, ceftizoxime, cefmenoxime--and a penicillin--n-formimidoyl thienamycin--by serial agar dilution method in the anaerobic glove box. there were 208 bacteroides isolates including 80 bacteroides fragilis and 100 peptococci and peptostreptococci. the minimal inhibitory concentration (mic) 75% of b ...	1984	6744975
bacteria in enteric lesions of cattle.	thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ...	1983	6829137
use of randomly cloned dna fragments for identification of bacteroides thetaiotaomicron.	randomly cloned fragments of dna from bacteroides thetaiotaomicron were used as hybridization probes for differentiation of b. thetaiotaomicron from closely related bacteroides species. hindiii digestion fragments of dna from b. thetaiotaomicron (type strain) were inserted into plasmid pbr322 and labeled with [alpha-32p]dctp by nick translation. these labeled plasmids were screened for hybridization to hindiii digests of chromosomal dna from type strains of the following human colonic bacteroide ...	1983	6833179
demonstration of bacteroides capsules by light microscopy and ultrastructural cytochemistry.	forty-six anaerobic gram-negative bacilli, including 26 members of the bacteroides fragilis group (bfg), were examined for capsules by the india ink technic. thirty-five were encapsulated, including all the bfg strains. as a follow-up, seven of these isolates and two previously studied reference strains (b. fragilis atcc 23745 and bacteroides vulgatus atcc 8482) were examined for capsules by ultrastructural cytochemistry. using the periodic acid thiocarbohydrazide silver proteinate (patcsp) meth ...	1983	6837522
metabolism of mercapturic acid-pathway metabolites of 2-chloro-n-isopropylacetanilide (propachlor) by gastrointestinal bacteria.	1. mercapturic acid pathway metabolites of propachlor, labelled with 14c, were incubated with pig caecal contents, small and large intestinal contents, and pure cultures of gastrointestinal bacteria. 2. the glutathione, cysteine, n-acetylcysteine, and the s-oxide of the n-acetylcysteine conjugates of propachlor (2-chloro-n-isopropylacetanilide) were each metabolized by mixed pig caecal micro-organisms to 2-mercapto-n-isopropylacetanilide. 3. the extent of formation of 2-mercapto-n-isopropylaceta ...	1983	6880239
bactericidal activity of a granule extract from human polymorphonuclear leukocytes against bacteroides species.	the microbicidal activity of an acetate extract of human polymorphonuclear leukocyte granules was tested against bacteroides fragilis, bacteroides vulgatus, bacteroides distasonis and bacteroides thetaiotaomicron. all strains tested were killed by the extract, and there were no significant differences between the different bacteroides species.	1983	6885166
properties of a new penicillinase type produced by bacteroides fragilis.	a highly penicillin-resistant strain of bacteroides fragilis, strain gn11499, was found among 80 clinical isolates of the b.fragilis group and appears to produce a new type of penicillinase. penicillinase activity was detected in crude extracts and had a specific activity of 0.25 u/mg of protein. about 20% of the enzyme was released into the surrounding medium during growth. the enzyme hydrolyzed ampicillin and cloxacillin more rapidly than it did penicillin g, carbenicillin, and cephaloridine. ...	1982	6983857
specific strains of bacteroides species in human fecal flora as measured by deoxyribonucleic acid homology.	membrane competition homology experiments were used to compare bacteroides uniformis and bacteroides vulgatus isolates obtained from fecal samples from different individuals and isolates obtained from fecal samples of single individuals. isolates of b. uniformis, when isolated from different individuals, had interstrain deoxyribonucleic acid homology values that ranged from 63 to 95%, with most of the values being in the 70 to 85% range. when isolates obtained from a single individual were compa ...	1980	6990866
effect of secreted bacteroides proteases on human intestinal brush border hydrolases.	selected bacteroides species secreted various amounts of protease and glycosidase into their growth medium. bacteroides vulgatus, distasonis, and ovatus secreted the most (31-60% of total). the secreted protease was similar in action to the protease within the organism, in that it had a broad ph optimum of 6-9, a k(m app.) for casein of 0.1 mum, and was inhibited by benzamidine, phenylmethylsulfonyl fluoride, diisopropylfluorophosphate (difp), and by an elastase inhibitor, ac(ala)(3)alach(2)cl. ...	1980	6995483
the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract.	the development of the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract was studied in 31 infants during the first 10 days of life. specimens were collected from the umbilicus, mouth and gastro-intestinal tract on the pre-operative day, at operation and on post-operative days 1, 2, 3, 5, 7 and 10. bacteria were isolated semi-quantitatively on a variety of plain and selective media and identified by conventional methods.staphylococcus albus was the predomin ...	1982	7057028
production of lesions in gnotobiotic mice by inoculation with treponema hyodysenteriae.	treponema hyodysenteriae was established in the ceca of gnotobiotic mice in the absence of other organisms. superficial mucosal lesions characteristic of swine dysentery were present in the ceca of mice inoculated with t. hyodysenteriae in combination with viable bacteroides vulgatus. deep crypt necrosis was detected in the ceca of mice inoculated with t. hyodysenteriae alone.	1981	7216455
production of experimental ulcerative colitis in gnotobiotic guinea pigs with simplified microflora.	conventional guinea pigs provided with a solution of 5% (wt/vol) degraded carrageenan as the sole source of oral fluids developed ulcerations of their ceca and large intestines within 30 days. similar lesions were not detected in germfree guinea pigs treated in an identical manner, suggesting that an intestinal microflora was necessary for development of intestinal lesions. to simplify the bacterial flora required for production of cecal ulcerations, 10 pools consisting of 10 bacterial strains e ...	1981	7216487
the cell-surface antigens of bacteroides vulgatus.	the cell surface of bacteroides vulgatus was examined by electron microscopy. the outer membrane complex was removed by edta and mild sonication and the antigens of this complex were characterized by enzyme-linked immunosorbent assay and crossed immunoelectrophoresis. the species-specific antigen was identified and was shown to be the major outer membrane protein with a molecular weight of 100 000.	1981	7334331
the toxicity of antigens extracted from strains of bacteroides vulgatus from different origins to chicken embryos.	the toxicity of purified lipopolysaccharides (lps) and capsular polysaccharides (cps) extracted from 3 strains of bacteroides vulgatus to 11 day old chicken embryos was examined. the three b. vulgatus strains were isolated from different sources. the celd50 (chicken embryos lethal dose 50%) of these preparations was determined. all antigens were shown to be lethal to chick embryos. the toxicity of lps of the strains isolated from clinical sources was higher than that of the capsular antigens. th ...	1994	7526620
the use of pcr to monitor the population abundance of six human intestinal bacterial species in an in vitro semicontinuous culture system.	six pcr primer sets complementary to the 16s rdnas (rrna genes) were developed and shown to be specific for the following anaerobic bacteria: clostridium clostridiiforme, c. perfringens, c. leptum, bacteroides vulgatus, b. distasonis, and b. thetaiotaomicron, respectively. these primers were used for pcr to detect and monitor the bacteria in a semicontinuous culture system designed to mimic intestinal microflora in the human gastrointestinal tract. except for c. perfringens, the five species of ...	1994	7529205
fibronectin and vitronectin binding of bacteroides fragilis and eight other species of the genus.	the fibronectin and vitronectin bindings of 152 strains belonging in 9 bacteroides species of different origins were tested by means of latex agglutination. 23% of the strains isolated from faeces exhibited fibronectin binding, as did 46% of the strains obtained from severe infections. most of the strains displaying fibronectin binding belonged to the species bacteroides fragilis or bacteroides vulgatus. the binding could be inhibited by preincubation of the cells with an excess amount of fibron ...	1994	7532044
analysis of bacteroides species by pyrolysis mass spectrometry.	two methods for the classification of isolates of the bacteroides fragilis group were compared in a study of 133 clinical isolates and 10 reference strains. there was broad similarity between the pattern of clusters in pyrolysis mass spectrometry (pyms), which reflects whole-cell composition, and that in conventional tests, which reflect nutritional interactions with the environment. a single large cluster was resolved by both approaches; this cluster comprised mainly strains identified as b. fr ...	1995	7548532
expression of a cloned cellulase/xylanase gene from prevotella ruminicola in bacteroides vulgatus, bacteroides uniformis and prevotella ruminicola.	a new shuttle vector, prh3 (8.7 kb), was constructed for use in prevotella/bacteroides host strains. this vector combines the prri2 replicon from p. ruminicola, pbluescript sequences and a tetq marker gene for selection in prevotella/bacteroides hosts. following insertion of a fragment carrying an endoglucanase/xylanase gene from p. ruminicola 23 into the multiple cloning site, the resulting construct, prh3x, was introduced into b. vulgatus 1447, b. uniformis 1100 and p. ruminicola 2202. this re ...	1995	7592134
immunocytochemical evidence of listeria, escherichia coli, and streptococcus antigens in crohn's disease.	infectious agents have long been suspected of playing a role in the initiation of crohn's disease. the objective of this study was to search for likely microbial agents in diseased tissues using immunocytochemical techniques.	1995	7729631
effect of h2-receptor antagonists on bile acid metabolism.	several reports have been presented concerning pronounced overgrowth of bacteria in gastric juices of patients treated with h2-receptor antagonists. however, there has been no report concerning influence of h2-receptor antagonists on jejunal flora. thus, to investigate the influence and its effect on bile acid metabolism, this study was performed: 1) to examine whether patients with gastric ulcers who have been treated with h2-receptor antagonists have positive bile acid breath tests due to bact ...	1995	7735920
adherence properties of bacteroides vulgatus, the preponderant colonic organism of adult humans.	bacteroides vulgatus is the predominant intestinal bacterium of adult humans. the adhesins of this species were studied by using haemagglutination of rabbit erythrocytes and inhibition of this haemagglutination. eleven haemagglutinating strains were tested after incubation with chicken albumin, bovine serum albumin, human serum fibronectin, fibronectin fragment and n-acetyl neuraminic acid, and after they had been heated, treated with naio4, trypsin and d(+)-mannose. rabbit erythrocytes treated ...	1994	7858350
origin of d-alanine present in urine of mutant mice lacking d-amino-acid oxidase activity.	urine of mutant ddy/dao- mice lacking d-amino-acid oxidase contained 13 times more d-alanine than that of normal ddy/dao+ mice. because d-alanine is a component of bacterial cell walls, the possibility that the urinary d-alanine came from intestinal bacteria was examined. in ddy/dao- mice that were made germ free at birth and reared in a germ-free environment, the quantity of urinary d-alanine was found to be at a low level comparable to that of the normal mice. when these germ-free mice were ma ...	1993	7901999
hematophagic histiocytosis: a clinicopathologic analysis of 23 cases with special reference to the association with peripheral t-cell lymphoma.	the clinicopathologic features of 23 patients with hematophagic histiocytosis (hh) are described. all of them exhibited increased histiocytes associated with hemophagocytosis in the marrow. the patients usually presented with fever, hepatosplenomegaly, lymphadenopathy, and cytopenia. the underlying illnesses were heterogeneous, including non-hodgkin's lymphoma in 17, systemic lupus erythematosus in one, diabetes mellitus in one, acute myelomonocytic leukemia in one, myelodysplastic syndrome in o ...	1994	7920083
susceptibility testing of dientamoeba fragilis atcc 30948 with iodoquinol, paromomycin, tetracycline, and metronidazole.	susceptibility testing was performed on dientamoeba fragilis atcc 30948 in a dixenic culture with klebsiella pneumoniae and bacteroides vulgatus. d. fragilis was cocultured with the bacteria in tygm-9 medium (atcc medium 1171). the activities of antiparasitic drugs were assessed by counting viable d. fragilis trophozoites with a hemacytometer by trypan blue exclusion. the minimal amebicidal concentrations of the following four drugs were determined: iodoquinol at 128 micrograms/ml, paromomycin a ...	1994	8067755
rapid detection of serpulina hyodysenteriae in diagnostic specimens by pcr.	a pcr assay for the detection of serpulina hyodysenteriae in diagnostic specimens was developed on the basis of sequence analysis of a recombinant clone designated pred3c6. clone pred3c6, which contained a 2.3-kb dna fragment unique to s. hyodysenteriae, was identified by screening a plasmid library of s. hyodysenteriae isolate b204 genomic dna in escherichia coli by colony immunoblot with the mouse monoclonal antibody 10g6/g10, which was produced against cell-free supernatant antigens from the ...	1994	8077394
sequence analysis of per-1 extended-spectrum beta-lactamase from pseudomonas aeruginosa and comparison with class a beta-lactamases.	we have determined the nucleotide sequence (embl accession number, z 21957) of the cloned chromosomal per-1 extended-spectrum beta-lactamase gene from a pseudomonas aeruginosa rnl-1 clinical isolate, blaper-1 corresponds to a 924-bp open reading frame which encodes a polypeptide of 308 amino acids. this open reading frame is preceded by a -10 and a -35 region consistent with a putative p. aeruginosa promoter. primer extension analysis of the per-1 mrna start revealed that this promoter was activ ...	1994	8141562
delta 22-beta-muricholic acid in monoassociated rats and conventional rats.	bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ...	1994	8177022
growth stimulator for bifidobacteria produced by propionibacterium freudenreichii and several intestinal bacteria.	a bifidogenic growth stimulator was present in the cell-free filtrate of propionibacterium freudenreichii 7025 culture and in the methanol extract fraction of the cells. several intestinal bacteria, such as bacteroides, enterobacter, and enterococcus, which also released a growth stimulator for bifidobacteria, may play an important role in regulation of a bifidobacterial population in colonic microflora. the water-soluble stimulator from the methanol extract of the cells was partially purified. ...	1994	8182163
antimicrobial resistance in human oral and intestinal anaerobic microfloras.	in the present study we determined the resistance patterns of anaerobic bacteria from human saliva and stool specimens and investigated whether there were significant differences in resistance between outpatients and hospitalized patients, regardless of whether they had received antimicrobial agents. no bacterial strains resistant to ampicillin, piperacillin, cefoxitin, cefuroxime, imipenem, clindamycin, doxycycline, chloramphenicol, or metronidazole were isolated from the saliva samples. howeve ...	1993	8215280
human t cell adhesion to endothelium stimulated by membrane components extracted from strains of bacteroides vulgatus (member of b. fragilis group).	bacteroides fragilis is reported to be less virulent than other gram negative rods, existing in normal gut flora (e.g. enterobacteriaceae). lymphocytic infiltration, a typical finding in chronic infection, depends on cell extravasation and trafficking towards a site of antigen, a process that is regulated by endothelial expression of adhesion molecules. we have studied the ability of lipopolysaccharides and capsular polysaccharides of b. vulgatus group to induce human t cell adhesion to cultured ...	1993	8239916
application of indirect immunofluorescence to detection of dientamoeba fragilis trophozoites in fecal specimens.	an indirect fluorescent-antibody (ifa) assay was carried out to examine for the presence of dientamoeba fragilis trophozoites in preserved fecal specimens. antiserum to d. fragilis trophozoites was raised in a rabbit with a dixenic culture of d. fragilis (atcc 30948) from the american type culture collection. after absorption with klebsiella pneumoniae and bacteroides vulgatus, the immune rabbit serum was used for examination by the ifa assay. a total of 155 clinical samples were tested; 42 with ...	1993	8349746
analysis of intestinal flora of a patient with congenital absence of the portal vein.	a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ...	1993	8364525
identification of a circular intermediate in the transfer and transposition of tn4555, a mobilizable transposon from bacteroides spp.	transmissible cefoxitin (fx) resistance in bacteroides vulgatus cla341 was associated with the 12.5-kb, mobilizable transposon, tn4555, which encoded the beta-lactamase gene cfxa. transfer occurred by a conjugation-like mechanism, was stimulated by growth of donor cells with tetracycline (tc), and required the presence of a bacteroides chromosomal tcr element. transconjugants resistant to either fx, tc, or both drugs were obtained, but only fxr tcr isolates could act as donors of fxr in subseque ...	1993	8386723
rape-seed meal toxicity in gnotobiotic rats: influence of a whole human faecal flora or single human strains of escherichia coli and bacteroides vulgatus.	gnotobiotic growing rats harbouring either a whole human faecal flora or single human strains of escherichia coli (em0) or bacteroides vulgatus (bv8h1) were fed for 7 weeks on semi-synthetic diets in which the protein source was either soya-bean meal (sm) or rape-seed meal (rm). for each bacterial status the rm-diet group was compared with the control group fed on the sm diet. the association of human faecal flora with the rm diet was responsible for reduced feed intake and reduced weight gain, ...	1993	8399112
characterization of the mobilization region of a bacteroides insertion element (nbu1) that is excised and transferred by bacteroides conjugative transposons.	many bacteroides clinical isolates carry large conjugative transposons that, in addition to transferring themselves, excise, circularize, and transfer smaller, unlinked chromosomal dna segments called nbus (nonreplicating bacteroides units). we report the localization and dna sequence of a region of one of the nbus, nbu1, that was necessary and sufficient for mobilization by bacteroides conjugative transposons and by incp plasmids. the fact that the mobilization region was internal to nbu1 indic ...	1993	8407836
genetic and biochemical analysis of a novel ambler class a beta-lactamase responsible for cefoxitin resistance in bacteroides species.	a clinical isolate of bacteroides vulgatus was resistant to tetracycline, clindamycin, ampicillin, cephaloridine, cefoxitin, and other beta-lactam antibiotics except imipenem. beta-lactam resistance was mediated by a membrane-associated, clavulanate-sensitive cephalosporinase capable of degrading cephalosporins and penicillins. cefoxitin also was degraded but at a slow rate. the cefoxitin resistance (fxr) determinant was cloned from b. vulgatus genomic libraries that were prepared in escherichia ...	1993	8517690
genetic analysis of the minimal replicon of plasmid pip417 and comparison with the other encoding 5-nitroimidazole resistance plasmids from bacteroides spp.	the nucleotide sequence of the dna replication origin region of a bacteroides vulgatus plasmid, pip417, encoding 5-nitroimidazole resistance has been determined. this region of 1934 bp presents some characteristics similar to those of other replication protein-dependent origins. it contains a large open reading frame which could encode a basic rep protein (repa) of 36.8 kda. upstream of this orf exist an at-rich region, three direct repeats (iterons) of 21 bp, multiple dnaa binding sites, and si ...	1995	8559801
the pathogenic role of bacteroides vulgatus in patients with ulcerative colitis.	to clarify the role played by bacteroides vulgatus in the pathogenesis of ulcerative colitis, the serum antibody titer to the outer membrane protein of b. vulgatus, obtained from the inflamed rectal mucosa, and measured by enzyme-linked immunosorbent assay, was investigated in patients with ulcerative colitis. high serum antibody titers to the outer membrane protein of b. vulgatus were found in these patients. the specific outer membrane protein of b. vulgatus reactive to serum antibody was reco ...	1995	8563888
selective enumeration of bacteroides vulgatus and b. distasonis organisms in the predominant human fecal flora by using monoclonal antibodies.	the genus bacteroides represents about one-third of the isolates from human fecal samples. the proportions of the different species are difficult to estimate because there is no method for rapid identification of mixtures of anaerobes. monoclonal antibodies against bacteroides vulgatus and b. distasonis were prepared. they did not react with the other bacteroides species of the b. fragilis group. these reagents allowed direct enumeration of b. vulgatus and b. diastasonis organisms in human fecal ...	1996	8593077
the in vitro activity of trospectomycin against anaerobic bacteria.	the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...	1995	8667035
pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples.	pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ...	1996	8919784