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qnrvc-like gene located in a novel complex class 1 integron harboring the iscr1 element in an aeromonas punctata strain from an aquatic environment in shandong province, china.a qnrvc-like gene, qnrvc4, was found in a novel complex class 1 integron gene cassette array following the iscr1 element and bla(per-1) in a multidrug-resistant strain of the aquatic bacterium aeromonas punctata. the deduced qnrvc4 protein sequence shares 45% to 81% amino acid identity with quinolone resistance determinants qnrb6, qnra1, qnrs1, qnrc, qnrvc1, and qnrvc3. a ser-83 to ile amino acid substitution in gyrase a may be mainly responsible for ciprofloxacin resistance in this strain.201020516288
biosynthesis and mobilization of a novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer produced by burkholderia sp. usm (jcm15050).we attempted to synthesize a polyhydroxyalkanoate (pha) containing newly reported 3-hydroxy-4-methylvalerate (3h4mv) monomer by using wild type burkholderia sp. usm (jcm15050) and its transformed strain harboring the pha synthase gene of aeromonas caviae (phac(ac)). the introduction of 3h4mv as a second monomer will improve the material properties of 3hb-based polymers. to promote the accumulation of pha containing 3h4mv monomer, isocaproic acid was provided as co-carbon source. approximately 1m ...201020541932
production of p(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) terpolymers using a chimeric pha synthase in recombinant ralstonia eutropha and pseudomonas putida.recombinant strains of ralstonia eutropha and pseudomonas putida harboring a chimeric polyhydroxyalkanoate (pha) synthase, which consisted of pha synthases of aeromonas caviae and r. eutropha, produced 3-hydroxybutyrate (3hb)-based pha copolymers comprised of 3-hydroxyhexanoate and 3-hydroxyoctanoate units from dodecanoate (87-97 mol % 3hb), indicating that the chimeric pha synthase possesses desirable substrate specificity leading to the production of 3hb-rich copolymers.201020699558
exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment.this study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (mfcs) using activated sludge for wine-containing wastewater treatment. phylogenetic tree analysis on 16s rrna gene fragments indicated that the predominant strains on anodic biofilm in acclimatized mfcs were gamma-proteobacteria aeromonas punctata niu-p9, pseudomonas plecoglossicida niu-y3, pseudomonas koreensis niu-x8, acinetobacter junii niu-y8, stenotropho ...201120859654
molecular mechanisms of quinolone resistance in clinical isolates of aeromonas caviae and aeromonas veronii bv. sobria.mutations in quinolone targets were studied together with quinolone efflux pump activation and plasmid-mediated quinolone resistance determinants in nalidixic-acid-resistant isolates of aeromonas caviae and aeromonas veronii. among 135 clinical aeromonas spp. isolated from stools of patients with gastrointestinal symptoms, 40 nalidixic acid-resistant strains belonging to a. caviae and a. veronii were selected and their susceptibility to different quinolones (ciprofloxacin, norfloxacin, ofloxacin ...201020890847
bacterial tolerance to silver nanoparticles (snps): aeromonas punctata isolated from sewage environment.use of silver nanoparticles (snps) is increasing in a large number of consumer products. thus, the possible build-up of the nanoparticles in the environment is becoming a major concern. aeromonas punctata isolated from sewage showed tolerance to 200 μg/ml snps. the growth kinetics data for a. punctata treated with nanoparticles were similar to those in the absence of nanoparticles. there was a reduction in the amount of exopolysaccharides (eps) in bacterial culture supernatant after nanoparticle ...201021077112
simple and direct detection of aeromonas hydrophila infection in the goldfish, carassius auratus (l.), by dot blotting using specific monoclonal antibodies.a combination of eight isolates of aeromonas hydrophila was used to produce monoclonal antibodies (mabs). ten different groups of mabs specific to aeromonas were selected. the first five groups of mabs demonstrated high specificity and bound to only one or two isolates of a. hydrophila. the sixth and the seventh groups of mabs were a. hydrophila specific. they recognized seven of eight a. hydrophila isolates (ah1, 2, 3, 4, 5, 6, 8); however, the mab in the seventh group also showed cross-reactiv ...201021091724
association of aeromonas caviae polar and lateral flagella with biofilm formation.to evaluate the association of the polar and lateral flagella with biofilm formation on plastic surfaces in 76 aeromonas caviae strains isolated from environment (lagoon water), food (vegetables, fish and cheese) and human source (faeces).201021118278
microbial production of polyhydroxyalkanoate block copolymer by recombinant pseudomonas putida.polyhydroxyalkanoate (pha) synthesis genes phapcj(ac) cloned from aeromonas caviae were transformed into pseudomonas putida ktoy06δc, a mutant of p. putida kt2442, resulting in the ability of the recombinant p. putida ktoy06δc (phapcj(a.c)) to produce a short-chain-length and medium-chain-length pha block copolymer consisting of poly-3-hydroxybutyrate (phb) as one block and random copolymer of 3-hydroxyvalerate (3hv) and 3-hydroxyheptanoate (3hhp) as another block. the novel block polymer was st ...201021181145
genome sequence of the emerging pathogen aeromonas caviae.aeromonas caviae is a gram-negative, motile and rod-shaped facultative anaerobe that is increasingly being recognized as a cause of diarrhea in children. here we present the first genome sequence of an a. caviae strain that was isolated as the sole pathogen from a child with profuse diarrhea.201021183677
formation of new polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer in burkholderia sp.burkholderia sp. synthase has been shown to polymerize 3-hydroxybutyrate (3hb), 3-hydroxyvalerate, and 3-hydroxy-4-pentenoic acid monomers. this study was carried out to evaluate the ability of burkholderia sp. usm (jcm 15050) and its transformant harboring the polyhydroxyalkanoate (pha) synthase gene of aeromonas caviae to incorporate the newly reported 3-hydroxy-4-methylvalerate (3h4mv) monomer. various culture parameters such as concentrations of nutrient rich medium, fructose and 4-methylval ...201121279348
application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents.a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ...201121419572
aeromonas spp. induce apoptosis of epithelial cells through an oxidant-dependent activation of the mitochondrial pathway.we investigated interactions of aeromonas caviae, aeromonas veronii biotype sobria and aeromonas hydrophila strains, isolated from faecal specimens of humans with gastroenteritis, with ht29 intestinal epithelial cells. all strains were found to be cytotoxic to the cells. bacterial infection caused generation of reactive oxygen species (ros) and nitric oxide radical (no(-à)). the maximal levels of ros and no(-à) were 14 and 35 times, respectively, greater in cells infected with aeromonas spp. tha ...201121436367
dissemination of ndm-1 positive bacteria in the new delhi environment and its implications for human health: an environmental point prevalence study.not all patients infected with ndm-1-positive bacteria have a history of hospital admission in india, and extended-spectrum β-lactamases are known to be circulating in the indian community. we therefore measured the prevalence of the ndm-1 gene in drinking water and seepage samples in new delhi.201121478057
phenotypic characteristics of human clinical and environmental aeromonas in western australia.: to determine the phenotypic characteristics of 199 aeromonas strains comprising 146 clinical and 53 environmental isolates.201121519288
identification and epidemiological relationships of aeromonas isolates from patients with diarrhea, drinking water and foods.a collection of aeromonas isolates obtained over a three-year period in the same geographic area (león, nw of spain) was characterized by (gtg)(5)-pcr fingerprinting, amplified fragment length polymorphism (aflp) analysis and gyrb gene sequence analysis. the isolates originated from human diarrheal stools (29 isolates), potable water (13 isolates), rabbit meat (13 isolates) and marine fish (5 isolates). the distribution of aeromonas species varied with the strain source. aeromonas caviae hg4 and ...201121550680
[Phenotypic characteristics and virulence factors in Aeromonas strains isolated from patients with diarrheic disease in Cuba].Fifty four strains of Aeromonas spp were isolated from patients with acute diarrheic episodes by using Aerokey II and Aeroesquema methods. In vitro antimicrobial susceptibility and virulence factors were analyzed. The most frequently isolated specie was Aeromonas caviae. Over 75% of strains exhibited resistance to penicillins and ce-phalosporins; for the other antibiotic groups resistance was under 20%. Twenty six strains (48.1 %) were multiresist-ant. At least one virulence factor among those e ...201121720696
in vitro antibacterial effect of aqueous and ethanolic moringa leaf extracts.to evaluate the antibacterial effect of aqueous and ethanolic moringa leaf extracts (moringa oleifera) on the growth of gram-positive and negative bacteria.201121771453
prevalence, characterization, and antimicrobial resistance of aeromonas strains from various retail food products in mumbai, india.ôçé a total of 154 food samples (chicken, fish, and ready-to-eat sprouts) from various retail outlets in mumbai, india, were analyzed for the presence of aeromonas spp. over a period of 2 y (january 2006 to march 2008). twenty-two aeromonas isolates belonging to 7 different species were isolated from 18 (11.7%) food samples. the highest percentages of isolation were from chicken (28.6%) followed by fish (20%) and sprout (2.5%) samples. aeromonas caviae, a. veronii bv. sobria, and a. salmonicida ...201121824136
isolation and partial characterization of a virulent bacteriophage ihq1 specific for aeromonas punctata from stream water.aeromonas punctata is the causative agent of septicemia, diarrhea, wound infections, meningitis, peritonitis, and infections of the joints, bones and eyes. bacteriophages are often considered alternative agents for controlling bacterial infection and contamination. in this study, we described the isolation and preliminary characterization of bacteriophage ihq1 (family myoviridae) active against the gram-negative bacterial strain a. punctata. this virulent bacteriophage was isolated from stream w ...201121947462
Bacteremia due to extended-spectrum-ß-lactamase-producing Aeromonas spp. at a medical center in Southern Taiwan.Although extended-spectrum-ß-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combinat ...201121968366
Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products.Aims: Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae (phaC(Ac) ) was transformed into this strain. Methods and Results: The resulting transformant incorporated approximately 1 ± 0·3 mol% of 3HHx ...201222054430
different clinical characteristics among aeromonas hydrophila, aeromonas veronii biovar sobria and aeromonas caviae monomicrobial bacteremia.this study aimed to compare the clinical presentations of aeromonas hydrophila, a. veronii biovar sobria and a. caviae monomicrobial bacteremia by a retrospective method at three hospitals in taiwan during an 8-yr period. there were 87 patients with a. hydrophila bacteremia, 45 with a. veronii biovar sobria bacteremia and 22 with a. caviae bacteremia. compared with a. hydrophila and a. veronii biovar sobria bacteremia, a. caviae bacteremia was more healthcare-associated (45 vs 30 and 16%; p = 0. ...201122065896
characterization and functional analyses of r-specific enoyl coenzyme a hydratases in polyhydroxyalkanoate-producing ralstonia eutropha.a genome survey of polyhydroxyalkanoate (pha)-producing ralstonia eutropha h16 detected the presence of 16 orthologs of r-specific enoyl coenzyme a (enoyl-coa) hydratase, among which three proteins shared high homologies with the enzyme specific to enoyl-coas of medium chain length encoded by phaj4 from pseudomonas aeruginosa (phaj4(pa)). the recombinant forms of the three proteins, termed phaj4a(re) to phaj4c(re), actually showed enoyl-coa hydratase activity with r specificity, and the catalyti ...201222081565
Development and validation of an HPLC-based screening method to acquire polyhydroxyalkanoate synthase mutants with altered substrate specificity.A rapid and convenient method for the compositional analysis of polyhydroxyalkanoate (PHA) was developed using high-performance liquid chromatography (HPLC) and alkaline sample pretreatment in a 96-well plate format. The reliability of this system was confirmed by the fact that a mutant with a D171G mutation of Aeromonas caviae PHA synthase (PhaC(Ac)), which gained higher reactivity toward 3-hydroxyhexanoate (3HHx), was selected from the D171X mutant library. Together with D171G mutant, several ...201122088761
adsorptive removal of silver nanoparticles (snps) from aqueous solution by aeromonas punctata and its adsorption isotherm and kinetics.silver nanoparticles (snps) are being increasingly used in many consumer products and industrial application. the release of snps to the environment is a major concern. here we have studied the adsorptive removal of snps by a snp resistant bacterial species aeromonas punctata, isolated from the sewage environment. the influence of zeta potential on adsorption was investigated at acidic, neutral and alkaline ph and with varying salt (nacl) concentrations. the rate of adsorption and removal of snp ...201122178439
distribution and phenotypic and genotypic detection of a metallo-β-lactamase, cpha, among bacteraemic aeromonas isolates.the objectives of the study were to investigate the distribution of cpha-related genes (cpha) encoding a cpha metallo-β-lactamase (mbl) among 51 consecutive aeromonas blood isolates and to compare different phenotypic methods for detecting cpha. the presence of cpha was detected by pcr. four phenotypic methods, the imipenem-edta combined disc test, imipenem-edta mbl etest, agar dilution test and modified hodge test (mht), were used to detect imipenem susceptibility and mbl production. the result ...201222322339
bacterial sepsis following prostatic biopsy.despite the recent innovations, complications of prostate biopsy can occur. the aim of this study was a prospective monitoring of major septic complications occurring after transrectal prostate biopsy, to describe their causing agents, to report the clinical course of these patients, and to give guidelines based on our personal experience.201222371127
air-dust-borne associations of phototrophic and hydrocarbon-utilizing microorganisms: promising consortia in volatile hydrocarbon bioremediation.aquatic and terrestrial associations of phototrophic and heterotrophic microorganisms active in hydrocarbon bioremediation have been described earlier. the question arises: do similar consortia also occur in the atmosphere? dust samples at the height of 15 m were collected from kuwait city air, and analyzed microbiologically for phototrophic and heterotrophic hydrocarbon-utilizing microorganisms, which were subsequently characterized according to their 16s rrna gene sequences. the hydrocarbon ut ...201222529000
high prevalence of qnr and aac(6')-ib-cr genes in both water-borne environmental bacteria and clinical isolates of citrobacter freundii in china.we investigated the prevalence of qnr and aac(6')-ib-cr genes in water-borne environmental bacteria and in clinical isolates of enterobacteriaceae, as well as the subtypes of qnr. environmental bacteria were isolated from surface water samples obtained from 10 different locations in hangzhou city, and clinical isolates of citrobacter freundii were isolated from several hospitals in four cities in china. qnra, qnrb, qnrs, and aac(6')-ib-cr genes were screened using pcr, and the genotypes were ana ...201122791048
molecular detection of the aeromonas virulence aerolysin gene in retail meats from different animal sources in egypt.meat commonly contain the same aeromonas spp. which occur in human diarrhoeal and non-diarrhoeal faecal samples. motile aeromonas were isolated from 5.6% of total 302 samples. the distribution of the isolates were 5.9 and 5.2% in fresh and frozen samples, respectively. of the 302 samples taken of the four animal meat species investigated, the genus aeromonas were isolated in 12.3% of the fresh samples collected from buffalo meat, in 6.5% of the samples collected from sheep meat and 14.0% from th ...201222806009
purification, characterization and amplification of a 1.8 kbp fragment of xylanase 5 from aeromonas caviae w-61.aeromonas caviae w-61 produces multiple extracellular xylanases, the xylanases 1, 2, 3, 4, and 5. in this study, we purified and characterized the xylanase 5 of a. caviae w-61, and amplified a part of xylanase 5 gene (xyn5). the purified xylanase 5 was found to be a single polypeptide with molecular mass of 140 kda. it was an endo-beta-1,4-xylanase showing optimum temperature 40 degrees c and optimum ph 6.0. xylobiose, xylotriose, xylotetrose, xylopentose, xylohexose and a small amount of xylose ...200222905388
identification of a putative glycosyltransferase responsible for the transfer of pseudaminic acid onto the polar flagellin of aeromonas caviae sch3n.motility in aeromonas caviae, in a liquid environment (in broth culture), is mediated by a single polar flagellum encoded by the fla genes. the polar flagellum filament of a. caviae is composed of two flagellin subunits, flaa and flab, which undergo o-linked glycosylation with six to eight pseudaminic acid glycans linked to serine and threonine residues in their central region. the flm genetic locus in a. caviae is required for flagellin glycosylation and the addition of pseudaminic acid (pse) o ...201222950021
chironomid egg masses harbour the clinical species aeromonas taiwanensis and aeromonas sanarellii.bacteria of the genus aeromonas are found worldwide in aquatic environments and may produce human infections. in 2010, two new clinical species, aeromonas sanarellii and aeromonas taiwanensis, were described on the basis of one strain recovered from wounds of hospitalized patients in taiwan. so far, only four environmental isolates of a. sanarellii and one of a. taiwanensis have been recorded from waste water in portugal and an additional clinical strain of a. taiwanensis from the faeces of a pa ...201222967097
active intermediates of polyhydroxyalkanoate synthase from aeromonas caviae in polymerization reaction.polyhydroxyalkanoate (pha) synthase from aeromonas caviae fa440 (phac(ac), baa21815) is one of the most valuable pha synthase, because of its function to synthesize a practical bioplastic, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)]. however, biochemical activity and active intermediates of phac(ac) have not been clarified until now. in the present study, a gene of phac(ac) was cloned and overexpressed by a cell-free protein expression system. both the polymerization a ...201223043466
isolation and identification of aeromonas caviae strain ks-1 as tbtc- and lead-resistant estuarine bacteria.tributyltin chloride (tbtc)- and lead-resistant estuarine bacterium from mandovi estuary, goa, india was isolated and identified as aeromonas caviae strain ks-1 based on biochemical characteristics and fame analysis. it tolerates tbtc and lead up to 1.0 and 1.4 mm, respectively, in the minimal salt medium (msm) supplemented with 0.4 % glucose. scanning electron microscopy clearly revealed a unique morphological pattern in the form of long inter-connected chains of bacterial cells on exposure to ...201323132753
aeromonas caviae is the most frequent pathogen amongst cases of aeromonas bacteremia in japan.aeromonas species can cause various infections including bacteremia, gastroenteritis, cholangitis, and wound infections. to date, most studies on aeromonas species have been reported from countries other than japan. the aim of this study, therefore, was to evaluate aeromonas bacteremia in japan.201323163893
in vitro evidence of chain transfer to tetraethylene glycols in enzymatic polymerization of polyhydroxyalkanoate.a polyhydroxyalkanoate (pha) was enzymatically synthesized in vitro, and the end structure of pha associated with a chain transfer (ct) reaction was investigated. in the ct reaction, pha chain transfers from pha synthase (phac) to a ct agent, resulting in covalent bonding of ct agent to the pha chain at its carboxyl end. in vitro ct reaction has never been demonstrated because of relatively low yields of in vitro synthesized poly[(r)-3-hydroxybutyrate)] (p(3hb)), which makes it difficult to char ...201323474615
molecular characterization of aeromonas species isolated from farmed eels (anguilla japonica).seventy aeromonas strains were identified by phylogenetic analysis using housekeeping genes (gyrb and rpod) in order to investigate etiological agents for aeromoniasis in farmed eels (anguilla japonica). the phylogenetic analysis showed that aeromonas aquariorum (n=22, 31.4%) was the predominant species among the investigated eel strains, followed by aeromonas caviae (n=16, 22.9%), a. veronii (n=13, 18.6%), a. hydrophila (n=12, 17.1%), a. jandaei (n=4, 5.7%), a. media (n=2, 2.9%), and a. trota ( ...201323499189
reclassification of aeromonas hydrophila subspecies anaerogenes.technological advances together with the continuous description of new taxa have led to frequent reclassifications in bacterial taxonomy. in this study, an extensive bibliographic revision, as well as a sequence analysis of nine housekeeping genes (cpn60, dnaj, dnax, gyra, gyrb, mdh, reca, rpob and rpod) and a phenotypic identification of aeromonas hydrophila subspecies anaerogenes were performed. all data obtained from previous physiological, phylogenetic, and dna-dna hybridization studies toge ...201323759598
re-identification of aeromonas isolates from chironomid egg masses as the potential pathogenic bacteria aeromonas aquariorum.egg masses of the non-biting midge chironomous sp. have recently been found to serve as a reservoir for vibrio cholerae and aeromonas species. these insects are widely distributed in freshwater and evidence suggests that they may disseminate pathogenic bacteria species into drinking water systems. in the current study the taxonomy of 26 aeromonas isolates, previously recovered from chironomid egg masses, was re-evaluated. it was found that 23 isolates, which had previously been identified as aer ...201123761256
necrotizing fasciitis caused by aeromonas caviae.aeromonads are rarely associated with human intestinal and extra-intestinal diseases and syndromes, ranging from relatively mild illnesses such as acute gastroenteritis to life-threatening conditions, including septicemia, necrotizing fasciitis, and myonecrosis. among the aeromonas species known to cause human infection, aeromonas caviae has been associated with septicemia and only one reported case of human soft tissue infection. most of the infections due to aeromonas occur in immunocompromise ...201223826556
aeromonas spp. simultaneously harbouring bla(ctx-m-15), bla(shv-12), bla(per-1) and bla(fox-2), in wild-growing mediterranean mussel (mytilus galloprovincialis) from adriatic sea, croatia.aeromonas species are becoming renowned as emerging pathogens by increasingly giving rise to a wide spectrum of food and waterborne infections in humans. another worrisome feature of aeromonads is the growing frequency of antibiotic resistance as a consequence of their prominent diversity in terms of resistance determinants. this study aimed at determining the antimicrobial resistance pattern, prevalence and characterization of acquired β-lactamases, including extended-spectrum-β-lactamases (esb ...201323973842
aeromonas punctata derived depolymerase that disrupts the integrity of klebsiella pneumoniae capsule: optimization of depolymerase production.formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health. extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells. using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system. the challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of klebsi ...201423996303
modification of β-oxidation pathway in ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil.ralstonia eutropha h16 is a useful platform for metabolic engineering aiming at efficient production of polyhydroxyalkanaotes being attracted as practical bioplastics. this study focused on bifunctional (s)-specific 2-enoyl-coa hydratase/(s)-3-hydroxyacyl-coa dehydrogenase encoded by fadb to obtain information regarding β-oxidation in this bacterium and to achieve compositional regulation of poly((r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate) [p(3hb-co-3hhx)] synthesized from soybean oil. in a ...201423999062
lectin-binding properties of aeromonas caviae strains.the cell surface carbohydrates of four strains of aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. with the exception of l-fucose and sialic acid, the sugar residues were detected in a. caviae strains. a marked difference, however, in the pattern of cell surface carbohydrates in different a. caviae isolates was observed. specific receptors for tritricum vulgaris (wga), lycopersicon esculentum ...200824031204
characterization of aeromonas species isolated from an estuarine environment.thirty water samples were collected, at two week intervals, from the estuary of the river cocó. the aim was to characterize the presence, distribution and types of aeromonas spp, in the estuary of the river cocó, ceara, brazil (03°46'28.83''s e 38°26'36.52''s). aeromonas were identified in 19 (63%) samples analyzed by plating and cfu counts. presence/absence tests were positive for 11 (37%) of the samples resulting in the detection of aeromonas in a total of 23 (77%) of samples. cfu counts varie ...201024031516
the occurrence of aeromonas in drinking water, tap water and the porsuk river.the occurrence of aeromonas spp. in the porsuk river, public drinking water and tap water in the city of eskisehir (turkey) was monitored. fresh water samples were collected from several sampling sites during a period of one year. total 102 typical colonies of aeromonas spp. were submitted to biochemical tests for species differentiation and of 60 isolates were confirmed by biochemical tests. further identifications of isolates were carried out first with the vitek system (biome˜rieux) and then ...201124031613
virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health.aeromonas spp. are ubiquitous aquatic organisms, associated with multitude of diseases in several species of animals, including fishes and humans. in the present study, water samples from two ornamental fish culture systems were analyzed for the presence of aeromonas. nutrient agar was used for aeromonas isolation, and colonies (60 no) were identified through biochemical characterization. seven clusters could be generated based on phenotypic characters, analyzed by the programme ntsyspc, version ...201224031887
aqu-1, a chromosomal class c β-lactamase, among clinical aeromonas dhakensis isolates: distribution and clinical significance.aeromonas dhakensis, a recently described aeromonas sp. formerly called aeromonas aquariorum, is associated with human infections. in this study, a chromosomal gene, blaaqu-1, was identified in a. dhakensis aak1 that constitutes a 1143-bp open reading frame and is 87% identical to the gene encoding ceph in aeromonas hydrophila. an escherichia coli top10 cell transformant harbouring blaaqu-1 was resistant to cefotaxime but not to cefepime. mrna expression of blaaqu-1 in the cefotaxime-resistant m ...201324055254
the in-vitro antibacterial effects of organic salts, chemical disinfectants and antibiotics against pathogens of black disease in fairy shrimp of thailand.the antibacterial effects of organic salts, chemical disinfectants and antibiotics were evaluated on cultures of aeromonas hydrophila c03, aeromonas sobria c26, a. sobria c29, aeromonas caviae c24 and acinetobacter sp. sh-94b, the pathogens that cause black disease found in fairy shrimps (streptocephalus sirindhornae sanoamuang et al. (2000) and branchinella thailandensis sanoamuang, saengphan & murugan) of thailand. the minimal inhibitory concentrations (mics) of organic salts (sodium chloride ...201424131294
seasonal incidence of and antibiotic resistance among aeromonas species isolated from domestic wastewater before and after treatment in stabilization ponds.the efficiency of stabilization pond treatment of domestic wastewater in removing culturable cells of motile aeromonas and its influence on the incidence of resistance to seven antibiotics were investigated in this study. removal efficiency was higher (p < 0.001) in the warm months (98.8%) than in the cold months (97%). among the 264 isolates, 163 were aeromonas caviae, 24 were a. hydrophila, and 54 were a. sobria. twenty-three isolates could not be identified to the species level. in the influe ...199224192933
cholera-like illness due to aeromonas caviae. 201324222288
emergence of vim-producing aeromonas caviae in israeli hospitals.resistance to carbapenems in aeromonas species is rare and mediated mostly by the chromosomal cpha gene. our aims were to describe the molecular characteristics of the first cases of vim-producing aeromonas caviae isolated from human samples.201424390932
molecular cloning and characterization of a toll receptor gene from macrobrachium rosenbergii.toll receptors are cell surface molecules acting as pattern recognition receptors (prrs) that have been implicated in the signaling pathway of innate immune responses. in this study, the full-length cdna of a toll receptor gene of macrobrachium rosenbergii, designated mrtoll, was successfully isolated using designed degenerate primers and the rapid amplification of cdna ends (race). the mrtoll gene sequence contained an open reading frame (orf) of 2799 nucleotides encoding a protein of 932 amino ...201424398262
biosynthesis of polyhydroxyalkanoate copolymers from methanol by methylobacterium extorquens am1 and the engineered strains under cobalt-deficient conditions.methylobacterium extorquens am1 has been shown to accumulate polyhydroxyalkanoate (pha) composed solely of (r)-3-hydroxybutyrate (3hb) during methylotrophic growth. the present study demonstrated that the wild-type strain am1 grown under co²⁺-deficient conditions accumulated copolyesters of 3hb and a c₅-monomer, (r)-3-hydroxyvalerate (3hv), using methanol as the sole carbon source. the 3hv unit was supposed to be derived from propionyl-coa, synthesized via the ethylmalonyl-coa pathway impaired b ...201424430207
biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) by recombinant escherichia coli expressing leucine metabolism-related enzymes derived from clostridium difficile.an obligate anaerobic bacterium clostridium difficile has a unique metabolic pathway to convert leucine to 4-methylvalerate, in which 4-methyl-2-pentenoyl-coa (4m2pe-coa) is an intermediate of this pathway. 4m2pe-coa is also able to be converted to 3-hydroxy-4-methylvalerate (3h4mv), a branched side chain monomer unit, for synthesis of polyhydroxyalkanoate (pha) copolymer. in this study, to synthesize 3h4mv-containing pha copolymer from leucine, the leucine metabolism-related enzymes (ldha and h ...201424484910
maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar t3ss export.bacterial swimming is mediated by rotation of a filament that is assembled via polymerization of flagellin monomers after secretion via a dedicated flagellar type iii secretion system. several bacteria decorate their flagellin with sialic acid related sugars that is essential for motility. aeromonas caviae is a model organism for this process as it contains a genetically simple glycosylation system and decorates its flagellin with pseudaminic acid (pse). the link between flagellin glycosylation ...201424527847
a neonate with a meningomyelocele complicated by aeromonas caviae ventriculoperitoneal shunt infection.we report on a newborn girl with a aeromonas caviae shunt infection and meningitis after insertion of a ventriculoperitoneal shunt and surgical repair of a meningomyelocele in one procedure. this pathogen has never been reported, related to ventriculoperitoneal shunt infections. beside the need for surgical revision of the shunt because of shunt obstruction and septa formation in the ventricles, the clinical outcome was good with intravenous cefotaxime therapy.201624579246
phasin proteins activate aeromonas caviae polyhydroxyalkanoate (pha) synthase but not ralstonia eutropha pha synthase.in this study, we performed in vitro and in vivo activity assays of polyhydroxyalkanoate (pha) synthases (phacs) in the presence of phasin proteins (phaps), which revealed that phaps are activators of phac derived from aeromonas caviae (phacac). in in vitro assays, among the three phacs tested, phacac was significantly activated when phaps were added at the beginning of polymerization (prepolymerization phacac), whereas the prepolymerization phacre (derived from ralstonia eutropha) and phacda (d ...201424584238
on the mechanism underlying tellurite reduction by aeromonas caviae st dihydrolipoamide dehydrogenase.the dihydrolipoamide dehydrogenase (lpda) from the tellurite-resistant bacterium aeromonas caviae st reduces tellurite to elemental tellurium. to characterize this nadh-dependent activity, the a. caviae lpda gene was subjected to site-directed mutagenesis and genes containing c45a, h322y and e354k substitutions were individually transformed into escherichia coli δlpd. cells expressing the modified genes exhibited decreased pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and tr activity re ...201424680738
quorum sensing activity of aeromonas caviae strain yl12, a bacterium isolated from compost.quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. in this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. isolate yl12 was identified using matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry and molecular typing using rpod gene which identified the iso ...201424759107
depolymerase improves gentamicin efficacy during klebsiella pneumoniae induced murine infection.presence of capsule enhances the virulence of bacteria that cause pneumonia, meningitis, cystic fibrosis, dental caries, periodontitis. capsule is an important virulence factor for klebsiella pneumoniae and infections due to this pathogen have been associated with high mortality rates. in the present study, use of an aeromonas punctata derived capsule depolymerase against k. pneumoniae, to reinstate the efficacy of gentamicin during pneumonia and septicemia was investigated.201425149315
expression of aeromonas punctata me-1 exo-xylanase x in e. coli for efficient hydrolysis of xylan to xylose.exo-xylanase x from aeromonas punctata me-1 was functionally expressed in escherichia coli with a carboxy terminal his tag (6×) and a molecular mass of 39.42 kda, which is in agreement with the prediction from its amino acid composition. the recombinant exo-xylanase reached 186 mg l(-1) after induction by isopropyl β-d-1-thiogalactopyranoside. its optimal temperature and ph were 50 °c and 6, respectively. the enzyme showed not only an exo-xylanase activity with k m of 3.90 mg ml(-1) and v max of ...201425213085
aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland.we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ...201525380369
distribution, virulence-associated genes and antimicrobial resistance of aeromonas isolates from diarrheal patients and water, china.to determine the prevalence of aeromonas infections in diarrheal patients, the distribution of virulence-associated genes and antibiotic resistance among different aeromonas species in china.201525447712
population dynamics and antimicrobial susceptibility of aeromonas spp. along a salinity gradient in an urban estuary in northeastern brazil.the main objective of this study was to quantify population and identify culturable species of aeromonas in sediment and surface water collected along a salinity gradient in an urban estuary in northeastern brazil. thirty sediment samples and 30 water samples were collected from 3 sampling locations (a, b and c) between october 2007 and april 2008. the aeromonas count was 10-7050cfu/ml (a), 25-38,500cfu/ml (b) and<10cfu/ml (c) for water samples, and ∼100-37,500cfu/g (a), 1200-43,500cfu/g (b) and ...201425455376
the aeromonas caviae aha0618 gene modulates cell length and influences swimming and swarming motility.aeromonas caviae is motile via a polar flagellum in liquid culture, with a lateral flagella system used for swarming on solid surfaces. the polar flagellum also has a role in cellular adherence and biofilm formation. the two subunits of the polar flagellum, flaa and flab, are posttranslationally modified by o-linked glycosylation with pseudaminic acid on 6-8 serine and threonine residues within the central region of these proteins. this modification is essential for the formation of the flagellu ...201425515520
occurrence, molecular characterization, and antimicrobial susceptibility of aeromonas spp. in marine species of shrimps cultured at inland low salinity ponds.we aimed to document the risk of aeromonas spp. in marine shrimp species cultured in inland low salinity ponds in thailand. in 14 of 18 shrimp samples retrieved from inland grow-up ponds, aeromonas spp. were detected at ranges from 4667 to 1,500,000 cfu/g body weight. the phylogenetic tree constructed with the gyrb and cpn60 concatenated sequences indicated that the 87 isolates consisted of aeromonas veronii (70%), aeromonas aquariorum (18%), aeromonas caviae (7%), aeromonas jandaei (2%), and ae ...201525583334
draft genome sequence of aeromonas caviae strain l12, a quorum-sensing strain isolated from a freshwater lake in malaysia.here, we present the draft genome sequence of aeromonas caviae strain l12, which shows quorum-sensing activity. the availability of this genome sequence is important to the research of the quorum-sensing regulatory system in this isolate.201525745006
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
regulation of 3-hydroxyhexanoate composition in phbh synthesized by recombinant cupriavidus necator h16 from plant oil by using butyrate as a co-substrate.a (r)-3-hydroxyhexanoate (3hh) composition-regulating technology for poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbh) production was developed using recombinant cupriavidus necator h16 with butyrate as a co-substrate. a new (r)-3-hydroxyhexanoyl-coa ((r)-3hh-coa) synthesis pathway was designed and enhanced by replacing the pha synthase gene (phac1) of c. necator by the phacacnsdg (encoding the n149s and d171g mutant of pha synthase from aeromonas caviae) and deactivation of the phaa gene (e ...201525805434
spontaneous bacterial peritonitis caused by aeromonas caviae in a patient with cirrhosis.spontaneous bacterial peritonitis (sbp) is a common complication of cirrhosis. based on our current understanding of sbp, the most common etiologies for sbp in cirrhosis are enterobacter and streptococcal species. th e aeromonas species are ubiquitous in fresh or sea water. aeromonas caviae is never identified as etiology in cases of sbp. a patient, who had a history of liver cirrhosis related to chronic hepatitis b virus infection for 1 year, presented with diarrhea. he had diarrhea 1 week late ...201525832540
construction of coa-dependent 1-butanol synthetic pathway functions under aerobic conditions in escherichia coli.1-butanol is an important industrial platform chemical and an advanced biofuel. while various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. here, we constructed a coa-dependent 1-butanol synthetic pathway that functions under aerobic conditions in escherichia coli, by expanding the previously reported (r)-1,3-butanediol synthetic pathway. the pathway consists of ...201525865277
draft genome sequence of aeromonas caviae 8lm, isolated from stool culture of a child with diarrhea.aeromonas spp. are gram-negative rods ubiquitous in aquatic environments; however, some species are able to cause a variety of infections in humans. here, we report the draft genome sequence of aeromonas caviae 8lm isolated from stool culture from a child with diarrhea in southern brazil.201525999559
a single-nucleotide substitution in phasin gene leads to enhanced accumulation of polyhydroxyalkanoate (pha) in escherichia coli harboring aeromonas caviae pha biosynthetic operon. 201526018503
[the relevance of correct identification and interpretation of susceptibility testing of aeromonas spp. bacteremia isolates].to assess the relevance of correct identification and interpretation of susceptibility testing of aeromonas spp. bacteremia isolates using newly developed molecular methods in comparison to previous conventional methods.201626027900
[pylephlebitis due to aeromonas caviae secondary to acute cholecystitis]. 201626031554
occurrence of bacteriophages infecting aeromonas, enterobacter, and klebsiella in water and association with contamination sources in thailand.the co-residence of bacteriophages and their bacterial hosts in humans, animals, and environmental sources directed the use of bacteriophages to track the origins of the pathogenic bacteria that can be found in contaminated water. the objective of this study was to enumerate bacteriophages of aeromonas caviae (aecaks148), enterobacter sp. (enspks513), and klebsiella pneumoniae (klpnks648) in water and evaluate their association with contamination sources (human vs. animals). bacterial host strai ...201526042992
aeromonas punctata derived depolymerase improves susceptibility of klebsiella pneumoniae biofilm to gentamicin.to overcome antibiotic resistance in biofilms, enzymes aimed at biofilm dispersal are under investigation. in the present study, applicability of an aeromonas punctata derived depolymerase capable of degrading the capsular polysaccharide (cps) of klebsiella pneumoniae, in disrupting its biofilm and increasing gentamicin efficacy against biofilm was investigated.201526063052
virulence and antimicrobial susceptibility of clinical and environmental strains of aeromonas spp. from northeastern brazil.the aims of the present study were to isolate and identify clinical and environmental strains of aeromonas spp. by means of biochemical tests and the automated method vitek 2 and to investigate the presence of the virulence genes cytotoxic enterotoxin (act), hemolysin (asa-1), and type iii secretion system (ascv), and also the in vitro antimicrobial susceptibility of the strains. from the clinical isolates, 19 aeromonas hydrophila, 3 aeromonas veronii bv. sobria, and 1 aeromonas caviae were iden ...201526103449
development of a pcr assay based on a single-base pair substitution for the detection of aeromonas caviae by targeting the gyrb gene.aeromonas caviae is a bacterial pathogen that causes various infectious diseases in both humans and animals. to facilitate its detection, we developed species-specific primer sets targeting polymorphisms in the gyrb gene for use in a pcr assay. the technique was able to detect 100% (29/29) of the a. caviae strains tested using either of two sets of primers (designated acf1-acr and acf3-acr), which produced 293-bp and 206-bp amplicons, respectively. another set of primers (designated acf2-acr) yi ...201526223267
comparative analysis of virulence genes, antibiotic resistance and gyrb-based phylogeny of motile aeromonas species isolates from nile tilapia and domestic fowl.the nucleotide sequence analysis of the gyrb gene indicated that the fish aeromonas spp. isolates could be identified as aeromonas hydrophila and aeromonas veronii biovar sobria, whereas chicken aeromonas spp. isolates identified as aeromonas caviae. pcr data revealed the presence of lip, ser, aer, act and cai genes in fish aer. hydrophila isolates, act, cai and aer genes in fish aer. veronii bv sobria isolates and ser and cai genes in chicken aer. caviae isolates. all chicken isolates showed va ...201526280543
newborn bacteraemia caused by an aeromonas caviae producing the vim-1 and shv-12 β-lactamases, encoded by a transferable plasmid. 201626410172
diarrhea outbreak in pernambuco, brazil, associated with a heat-stable cytotoxic enterotoxin produced by aeromonas caviae.in the present study enterotoxic and cytotoxic activities of twenty aeromonas caviae strains were examined. they originated from fecal specimens of patients with acute diarrhea during an outbreak in brazil in 2004. culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in vero and caco-2 cells. the enterotoxic and cytotoxic factors were heat-stable after culture supernatants treat ...201526422161
draft genome sequence of aeromonas caviae strain 429865 inp, isolated from a mexican patient.aeromonas caviae is an emerging human pathogen. here, we report the draft genome sequence of aeromonas caviae strain 429865 inp which shows the presence of various putative virulence-related genes.201526494682
analysis of the structure and function of fox-4 cephamycinase.class c β-lactamases poorly hydrolyze cephamycins (e.g., cefoxitin, cefotetan, and moxalactam). in the past 2 decades, a new family of plasmid-based ampc β-lactamases conferring resistance to cefoxitin, the fox family, has grown to include nine unique members descended from the aeromonas caviae chromosomal ampc. to understand the basis for the unique cephamycinase activity in the fox family, we determined the first x-ray crystal structures of fox-4, apo enzyme and the acyl-enzyme with its namesa ...201626525784
quinolone resistant aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewater.members of the genus aeromonas are recognized carriers of antibiotic resistance in aquatic environments. however, their importance on the spread of resistance from hospital effluents to the environment is poorly understood. quinolone resistant aeromonas spp. (n = 112) isolated from hospital effluent (he) and from raw (rww) and treated wastewater (tww) of the receiving urban wastewater treatment plant (uwtp) were characterized. species identification and genetic intraspecies diversity were assess ...201626546762
compositional regulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by replacement of granule-associated protein in ralstonia eutropha.phasin (phap), a kind of polyhydroxyalkanoate (pha) granule-associated proteins, has a role in controlling the properties of pha granules surface, and is thought to have influence on pha biosynthesis in pha-producing bacteria. this study focused on the phap1(re) locus in ralstonia eutropha as a site of chromosomal modification for production of flexible poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] from soybean oil.201526597300
availability of safe drinking-water: the answer to cholera outbreak? nabua, camarines sur, philippines, 2012.in may 2012, there were increasing diarrhoea cases and deaths reported from nabua, camarines sur to the philippines event-based surveillance system. an investigation was conducted to identify risk factors and determine transmission dynamics.201526668761
characterisation of a chromosomally-encoded extended-spectrum β-lactamase gene blaper-3 in aeromonas caviae of chicken origin. 201626674256
characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein pham from ralstonia eutropha.the binding preference of a polyhydroxyalkanoate (pha) biosynthesis-related multifunctional protein from ralstonia eutropha (phamre) was characterized. in vitro activity assay showed that pha synthase from r. eutropha (phacre) was activated by the presence of phamre but pha synthase from aeromonas caviae (phacac) was not. additionally, in vitro assays of protein-protein interactions demonstrated that phamre interacted with phacre directly, but did not interact with phacac. these results suggest ...201626728018
evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products.antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ...201026781242
effect of antibiotic supplementation on the quality of cryopreserved fish sperm of silver barb (barbodes gonionotus): sperm motility and viability, bacterial quality and fertilization.disease prevention is a key aspect in developing cryopreservation procedures for fish sperm and in improving the reproductive biotechnology for commercially important aquatic species. the purpose of this study was to evaluate the effects of an antibiotic supplementation (0.25% penicillin-streptomycin, ps and 0.25% penicillin-gentamicin, pg) on sperm motility and viability, bacterial profile and fertilization capacity of cryopreserved silver barb (barbodes gonionotus) semen. the experimental prot ...201626786020
antibiogram characterization and putative virulence genes in aeromonas species isolated from pig fecal samples.aeromonas species are broadly distributed in nature and agricultural environments and have been isolated from feces, bedding, and drinking water of healthy pigs. we assessed the incidence, virulence properties, and antimicrobial resistance profile of aeromonas spp., isolated from pig feces. antibiogram was done using the disc diffusion methods, and polymerase chain reaction was used for the detection of putative virulence genes. identification of isolates revealed three phenotypic species with p ...201626971520
genotypic and phenotypic identification of aeromonas species and cpha-mediated carbapenem resistance in queensland, australia.infection caused by aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. carbapenem resistance due to metallo-beta-lactamase, cpha encoded by the cpha gene, is a significant problem. this study defines aeromonas spp. causing clinical disease in queensland, australia. phenotypic tests for carbapenemase detection were assessed. one hundred aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by ...201626971634
unusual microorganisms and antimicrobial resistances in a group of syrian migrants: sentinel surveillance data from an asylum seekers centre in italy.three years of civil war in syria have caused death and increase of communicable diseases. the suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps.201726987764
[infection status and virulent genes of aeromonas in diarrhea patients in pudong new area, shanghai].to investigate the infection status and virulent genes of aeromonas in patients with acute diarrhea in pudong new area, shanghai.201627005546
impact of stress on aeromonas diversity in tambaqui (colossoma macropomum) and lectin level change towards a bacterial challenge.tambaqui (colossoma macropomum) is among the most cultivated fish species in tropical countries. stress is the main cause of disease in fish farms. the genus aeromonas is a common causative agent of fish diseases. this work reports the identification of aeromonas species colonizing gills of c. macropomum submitted or not to a confinement stress. we also evaluated changes in serum levels of lectins (carbohydrate-binding proteins that are components of fish immune system) in tambaqui submitted to ...201627046480
a study on the relation between poly(3-hydroxybutyrate) depolymerases or oligomer hydrolases and molecular weight of polyhydroxyalkanoates accumulating in cupriavidus necator h16.cupriavidus necator h16 has nine genes of poly(3-hydroxybutyrate) (phb) depolymerases or oligomer hydrolases (intracellular phb mobilization enzymes). in this study, we evaluated the relation between these genes and the accumulation, consumption, and molecular weight of polyhydroxyalkanoates (phas) accumulating in strain h16 and in a recombinant c. necator strain, knk-005, which harbors an nsdg mutant of the pha synthase gene (phacac) from aeromonas caviae. phaz6 had a significant influence on t ...201627059479
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