Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| glyceraldehyde-3-phosphate dehydrogenase of edwardsiella tarda has protective antigenicity against vibrio anguillarum in japanese flounder. | edwardsiella tarda glyceraldehyde-3-phosphate dehydrogenase (gapdh) may be an effective vaccine candidate against infection by e. tarda in japanese flounder paralichthys olivaceus. the gapdh of e. tarda is highly homologous to that of vibrio cholerae (91%), and therefore e. tarda gapdh may have protective antigenicity against vibrio species. in this study, we immunized japanese flounder with gapdh of e. tarda and infected the fish with v anguillarum. the result showed that gapdh prepared from e. ... | 2007 | 17629116 |
| edwardsiella tarda septicemia with underlying multiple liver abscesses. | edwardsiella tarda has recently been described as a member of the family enterobacteriaceae. the genus edwardsiella contains three species; e. hoshinae, e. ictaluri and e. tarda. edwardsiella tarda is the only species which has been recognised as pathogenic to humans, especially in those with an underlying disease. the most common presentation is watery diarrhoea. extra intestinal infections have been reported infrequently. humans seem to be infected or colonised with edwardsiella through ingest ... | 2006 | 17694959 |
| base changes in the flic gene of edwardsiella tarda: possible effects on flagellation and motility. | edwardsiella tarda is a broad host-range pathogen infecting both animals and humans. e. tarda isolates from red sea bream pagrus major are non-motile, whereas isolates from japanese eel anguilla japonica and japanese flounder paralichthys olivaceus are motile with peritrichous flagella. we compared the flic gene coding for flagellin (flic) in motile and non-motile e. tarda strains isolated from diseased fish. twenty-two amino acid residues differed in the predicted flic amino acid sequences betw ... | 2007 | 17760384 |
| dissection of a type vi secretion system in edwardsiella tarda. | bacterial pathogens use different protein secretion systems to deliver virulence factors. recently, a novel secretion system was discovered in several gram-negative bacterial pathogens, and was designated as the type vi secretion system (t6ss). in edwardsiella tarda, a partial e. tardavirulent protein (evp) gene cluster was implicated in protein secretion. here, we identified the entire evp cluster as a t6ss and two additional secreted proteins (evpi, a homologue of vgrg, and evpp) were found. w ... | 2007 | 17986187 |
| gastroenteritis caused by edwardsiella tarda in a pediatric renal transplant recipient. | edwardsiella tarda, a member of the family enterobacteriaceae, is a gram-negative bacillus that is most often pathogenic in aquatic environments. human infections with edwardsiella are rare, with most occurring in immunocompromised or immunosuppressed hosts. reported infections include meningitis, cholecystitis, endocarditis, osteomyelitis, soft tissue infections, bacteremia and septicemia, dysentery, and gastroenteritis. this report describes a case of e. tarda gastroenteritis in a renal transp ... | 2008 | 18086238 |
| comparison of the responses of peritoneal macrophages from japanese flounder (paralichthys olivaceus) against high virulent and low virulent strains of edwardsiella tarda. | in vivo infection studies in japanese flounder (paralichthys olivaceus) demonstrated that the number of viable cells of the virulent strain (nuf251) of edwardsiella tarda increased gradually in kidney and hepato-pancreas after intraperitoneal injection, but the low virulent strain (nuf194) did not. to gain insight into the virulence factors of e. tarda, in vitro responses of japanese flounder (p. olivaceus) peritoneal macrophages to these strains were compared in terms of phagocytosis, bacterici ... | 2008 | 18178102 |
| characterization of the rrn operons in the channel catfish pathogen edwardsiella ictaluri. | to advance diagnostics and phylogenetics of edwardsiella ictaluri by sequencing and characterizing its rrn operons. | 2008 | 18217940 |
| phenotypic and genetic characterization of edwardsiella tarda isolated from pond sediments. | the extent of genotypic and phenotypic diversity of edwardsiella tarda isolated from pond sediment was assessed by sds-page, plasmid profiling and eric-pcr. sds-page of whole cell protein extracts reveals 20-23 discrete bands with molecular wt of 14-110 kda. several bands with molecular weight range of 38-83 kda were present in all the isolates. numerical analysis of protein electrophoregram delineated the isolates into four clusters. two different types of plasmids having molecular mass of 23 k ... | 2009 | 18243313 |
| isolation of an unusual strain of edwardsiella tarda from turbot and establish a pcr detection technique with the gyrb gene. | the aim of this study was to report an unusual edwardsiella tarda and develop an effective method to identify this bacterium. | 2008 | 18341558 |
| the macrophage chemotactic activity of edwardsiella tarda extracellular products. | the chemoattractant capabilities of edwardsiella tarda extracellular products (ecp) were investigated from two isolates, the virulent fl6-60 parent and less virulent ret-04 mutant. chemotaxis and chemokinesis were assayed in vitro using blind well chambers with peritoneal macrophages obtained from nile tilapia, oreochromis niloticus, 5 days following squalene injection. non-purified ecp derived from both isolates stimulated predominantly chemokinetic migration of macrophages. additionally, the e ... | 2008 | 18355182 |
| the type iii secretion system-dependent repression of nf-kappab activation to the intracellular growth of edwardsiella tarda in human epithelial cells. | edwardsiella tarda is a pathogen with a broad host range infecting animals and humans. we have reported recently that the type iii secretion system (ttss) is essential for intracellular replication of the bacterium in murine macrophages. the present study shows that the ttss is also needed for intracellular growth of the bacterium in human epithelial cells (hep-2). however, different from the previous microarray analyses on murine macrophages, upregulation of the mrna expression level of nf-kapp ... | 2008 | 18397289 |
| secretory delivery of heterologous proteins in attenuated vibrio anguillarum for potential use in vaccine design. | to synthesize and secrete heterologous proteins in an attenuated vibrio anguillarum strain for potential multivalent live vaccine development, different antigen-delivery systems based on bacterial-originated secretion signal peptides (sps) were designed and identified in this work. four sps were derived from hemolysin of escherichia coli, rtx protein of v. cholerae, hemolysin of v. anguillarum, zinc-metalloprotease of v. anguillarum, respectively, and their abilities to support secretion of gree ... | 2008 | 18535826 |
| teleostean il11b exhibits complementing function to il11a and expansive involvement in antibacterial and antiviral responses. | interleukin 11 is a class-1 helical cytokine, having the four-helix bundle structure, possessing pleiotropic characteristics involved in physiological processes including blood production, bone formation and placentation. the interleukin 11 paralogues (il11a and il11b) have been identified in fish with only il11a from carp and trout have been characterized and analyzed for its expression thus far. here, we cloned and studied the structure and expression of il11b in japanese flounder (paralichthy ... | 2008 | 18538848 |
| chemical investigation of finlaysonia obovata: part i--a rare triterpene acid showing antibacterial activity against fish pathogens. | the antibacterial screening of extracts of the leaves of finlaysonia obovata with hexane, chloroform and alcohol was carried out against fresh water fish pathogenic bacteria viz., micrococcus sp. (multidrug resistant strain), aeromonas hydrophila, pseudomonas aeruginosa, vibrio alginolyticus, staphylococcus aureus, escherichia coli, edwardsiella tarda by disc-assay method. the hexane and chloroform extracts were found active against four and five pathogens, respectively. the highly active chloro ... | 2008 | 18569719 |
| regulation of autoinducer 2 production and luxs expression in a pathogenic edwardsiella tarda strain. | edwardsiella tarda is a bacterial pathogen that can infect both humans and animals. tx1, an ed. tarda strain isolated from diseased fish, was found to produce autoinducer 2 (ai-2)-like activity that was growth phase dependent and modulated by growth conditions. the gene coding for the ai-2 synthase was cloned from tx1 and designated luxs(et)(.) luxs(et) was able to complement the ai-2 mutant phenotype of escherichia coli strain dh5alpha. expression of luxs(et) correlated with ai-2 activity and w ... | 2008 | 18599834 |
| development of an effective edwardsiella tarda vaccine for cultured turbot (scophthalmus maximus). | since 2004 edwardsiella tarda has become one of the most important emerging pathogens in turbot aquaculture industry in europe causing serious economic losses. therefore, this study aimed to design an effective vaccination strategy to prevent edwardsiellosis in this fish species. two vaccine formulations, an adjuvanted vaccine and an aqueous bacterin, and different routes of administration, bath and intraperitoneal injection (i.p.), were tested. the effectiveness of the different immunization st ... | 2008 | 18602276 |
| molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic edwardsiella tarda strain. | the gene encoding the edwardsiella tarda ferric uptake regulator (fur(et)) was cloned from a pathogenic e. tarda strain isolated from diseased fish. fur(et) shares 90% overall sequence identity with the escherichia coli fur (fur(ec)) and was able to complement the mutant phenotype of a fur (ec)-defective e. coli strain. mutational analysis indicated that c92s and c95s mutations inactivated fur(et) whereas e112k mutation resulted in a superactive fur(et) variant. fur(et) negatively regulated its ... | 2008 | 18604507 |
| differential modulations of two glyceraldehyde 3-phosphate dehydrogenase mrnas in response to bacterial and viral challenges in a marine teleost oplegnathus fasciatus (perciformes). | glyceraldehyde 3-phosphate dehydrogenase (gapdh) has been recognized as an invariant internal control for various gene expression studies due to its classical housekeeping role in the glycolytic metabolism. however recently, this enzyme has been proven to be a multifunctional protein involved in diverse non-glycolytic activities. in the present study, two paralogue isoforms of gapdh mrnas were characterized from a marine teleost species, rockbream (oplegnathus fasciatus; perciformes) and their t ... | 2008 | 18692141 |
| production of monoclonal antibodies specific to major outer membrane protein of edwardsiella tarda. | edwardsiella tarda is an important cause for hemorrhagic septicemia in fish and gastro and extra-intestinal infections in humans. monoclonal antibodies (mabs) were produced against outer membrane proteins (omps) of e. tarda et-7, isolated from diseased snakehead (ophiocephalus punctatus). two stable hybridoma clones, designated as 3f10 and 2c3 mabs were found to be potentially specific for e. tarda by indirect enzyme linked immunosorbent assay (elisa). these mabs recognized major immunogenic omp ... | 2010 | 18804863 |
| development of qcm biosensor to detect a marine derived pathogenic bacteria edwardsiella tarda using a novel immobilisation method. | qcm technology offers a real time output, simplicity of use and cost effectiveness in addition to high sensitivity. sensitivity of qcm immunosensor can be enhanced by improving the immobilisation procedure on the quartz surface. the immobilisation strategy should be able to control both the amount and the orientation of the antibody (immunoglobulin; igg) on the transducer for high affinity to antigens. this study introduced a new methodology recruiting oxidised igg to expose aldehyde group in fc ... | 2009 | 18838265 |
| influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion. | the first step of the pathogenesis of many infectious diseases is the colonisation of the mucosal surface by the pathogen. bacterial colonisation of the mucosal surface is promoted by adherence to high molecular weight mucus glycoproteins. we examined the effect of carp intestinal mucus glycoproteins on the adhesion of different bacteria. the bacteria used were 3 strains of aeromonas hydrophila, and a. salmonicida, edwardsiella tarda and yersinia ruckeri. all bacteria adhered to mucus, but at va ... | 2008 | 18924378 |
| development of monoclonal antibodies to rohu [labeo rohita] immunoglobulins for use in immunoassays. | serum immunoglobulins [ig] of rohu [labeo rohita] were purified by affinity chromatography using bovine serum albumin as capture ligand. the purified rohu ig [r-ig] had a molecular weight [mw] of 880 kda as determined with gel filtration chromatography. the heavy chain of r-ig had an mw of 77.8 kda and that of light chain was 26.4 kda in sds-page. purified r-ig was used for the production of two anti-rohu ig monoclonal antibodies [d7 and h4] that belonged to subclass igg2b and igg1, respectively ... | 2008 | 18996722 |
| generation of safety enhanced edwardsiella tarda ghost vaccine. | a dual vector expressing the ghost-inducing phix174 lysis e gene and the bacterial dna degrading staphylococcal nuclease a (sna) gene was constructed to solve the problem of remnant antibiotic resistance genes and genomic dna with intact pathogenic islands in the final product of edwardsiella tarda ghosts (etg). the sna (devoid of secretion signal sequence and the nuclease b amino terminus sequence), fused with the 26 amino acid n-terminal sequence of the lambda phage cro gene, showed successful ... | 2008 | 18998589 |
| growth differentiation factor 15, a novel acute phase response gene in japanese flounder, paralichthys olivaceus. | the acute phase response, an important aspect of innate immunity, leads to the production of acute phase proteins (apps) in the liver which would consequently help restore homeostasis to the body. here, we identified a novel cytokine, growth differentiation factor 15 (gdf15) from japanese flounder. three out of the 384 est sequences derived from liver of japanese flounder treated with formalin-killed edwardsiella tarda showed significant homology with gdf of various species. after obtaining the ... | 2009 | 19059342 |
| identification and characterization of the prepro-vasoactive intestinal peptide gene from the teleost paralichthys olivaceus. | the gene encoding the prepro form of a vasoactive intestinal peptide (vip) was cloned, and its structural organization and expression profiles were determined in the teleost paralichthys olivaceus. the prepro-vip encodes both a vip and a peptide-histidine-isoleucine (phi). the vip gene comprises six exons with two distinct peptides encoded on separate exons, i.e., phi on exon iii and vip on exon iv. several elements involved in cytokine-mediated activation are highly conserved in an 806-bp segme ... | 2009 | 19084281 |
| a 12-year-old boy with acute gastroenteritis caused by edwardsiella tarda o4:h4. | a 12-year-old boy was brought to the hospital with a 3-week history of watery diarrhea mixed with mucus and colicky abdominal pain. stool culture identified edwardsiella tarda o4: h4, and no other pathogenic bacteria were detected. acute gastroenteritis caused by edwardsiella tarda o4: h4 was diagnosed. this bacterium was shown to be sensitive to ampicillin hydrate. when this antibiotic was administered, the condition of the patient improved within a week. the patient had a history of eating raw ... | 2008 | 19089557 |
| mice orally vaccinated with edwardsiella tarda ghosts are significantly protected against infection. | bacterial ghosts may be generated by the controlled expression of the phix174 lysis gene e in gram-negative bacteria and they are intriguing vaccine candidates since ghosts retain functional antigenic cellular determinants often lost during traditional inactivation procedures. the edwardsiella tarda ghost (etg) vaccine was prepared using this technology and tested in vaccination trials. control groups included mice immunized with formalin-killed e. tarda (fkc) or mice treated with phosphate-buff ... | 2009 | 19166892 |
| response of mucosal and systemic sigm-positive cells in turbot (scophthalmus maximus l.) immunization with edwardsiella tarda. | indirect fluorescence analyze technology (ifat) and fluorescence-activated cell sorter (facs) analysis were used to detect surface igm positive cells (sigm+ cells) in lymphoid tissues of unvaccinated turbots and vaccinated ones with inactivated edwardsiella tarda. the results were as follows: (1) the percentage of sigm+ cells respectively was 0+/-0.00% of skin, 1.58+/-0.40% of gills, 17.05+/-0.39% of peripheral blood (pbl), 21.06+/-1.79% of kidney in unvaccinated healthy turbots (average length: ... | 2009 | 19178953 |
| evaluation of a digoxigenin-labelled probe for detection of aeromonas spp. | an outer membrane protein-based digoxigenin (dig)-labelled dna probe was developed for the specific detection of aeromonas sp. from food/environmental/clinical samples. dot blot reaction answered for all the aeromonas isolates and was negative for escherichia coli, pseudomonas sp., klebsiella sp., vibrio parahaemolyticus, v. harveyi, v. alginolyticus, v. vulnificus. edwardsiella tarda and staphylococcus sp. as this protein is highly conserved in various aeromonas species, the probe has the poten ... | 2009 | 19187505 |
| multiple brain abscesses in neonate caused by edwardsiella tarda: case report. | a neonate presented with multiple brain abscesses caused by very unusual infection with the gram-negative bacterium, edwardsiella tarda. serial changes in magnetic resonance imaging findings including diffusion-weighted imaging demonstrated the development from the late cerebritis to late capsule stages. the patient was successfully treated by external drainage, and has since reached normal development milestones. early diagnosis with computed tomography, magnetic resonance imaging, and ultrasou ... | 2009 | 19246871 |
| in this issue: proteomics 5/2009. | in this issue of proteomics you will find the following highlighted articles:heart (pump) broken?hearts are pumps within pumps within channels and pumps. calcium is pumped, potassium, sodium, amino acids, and electrons are all pumped, channeled or driven until, finally, blood is pumped. failure of one or more pumps leads to a heart attack. this report from zlatkovic et al. looks at the sub-proteome associated with hypertensive failure of the k(+)atp channel and associated cardiomyopathy that dev ... | 2009 | 19253291 |
| emerging infections of the gastrointestinal tract. | infections account for significant gi morbidity and mortality worldwide. new organisms are being identified, associated with diarrhoeal illness and some with other gastrointestinal illness as well. among gi viruses, sapovirus is now recognised to cause diarrhoea, especially in children. a hypervirulent strain of clostridium difficile has caused epidemics in many countries. newly identified bacterial species that may cause diarrhoea include campylobacter concisus, arcobacteria, edwardsiella tarda ... | 2009 | 19258189 |
| immunoprotective analysis of two edwardsiella tarda antigens. | 2009 | 19282634 | |
| characterization of edwardsiella tarda rpos: effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. | in this study, rpos gene was identified from edwardsiella tarda eib202 and its functional role was analyzed by using an in-frame deletion mutant rpos and the complemental strain rpos (+). compared with the wild type and rpos (+), rpos was impaired in terms of the ability to survive under oxidative stress and nutrient starvation, as well as the resistance to 50% serum of scophthalmus maximus in 3 h, demonstrating essential roles of rpos in stress adaptation. the rpos mutant also displayed markedl ... | 2009 | 19283379 |
| identification of novel putative virulence factors, adhesin aida and type vi secretion system, in atypical strains of fish pathogenic edwardsiella tarda by genomic subtractive hybridization. | edwardsiella tarda, which is known to be the causative agent of edwardsiellosis in freshwater and marine fish, has two motility phenotypes. typical strains exhibiting motility are isolated mainly from freshwater fish and japanese flounder. atypical strains exhibiting non-motility are isolated mainly from marine fish, with the exception of japanese flounder. subtractive hybridization was performed to identify genomic differences between these two phenotypes. two fragments which showed homology to ... | 2009 | 19302523 |
| investigation of esca as a chaperone for the edwardsiella tarda type iii secretion system putative translocon component esec. | edwardsiella tarda is an important gram-negative enteric pathogen affecting both animals and humans. it possesses a type iii secretion system (t3ss) essential for pathogenesis. eseb, esec and esed have been shown to form a translocon complex after secretion, while escc functions as a t3ss chaperone for eseb and esed. in this paper we identify esca, a protein required for accumulation and proper secretion of another translocon component, esec. the esca gene is located upstream of esec and the esc ... | 2009 | 19332827 |
| attenuation of edwardsiella tarda virulence by small peptides that interfere with luxs/autoinducer type 2 quorum sensing. | edwardsiella tarda is a gram-negative pathogen with a broad host range that includes humans, animals, and fish. recent studies have shown that the luxs/autoinducer type 2 (ai-2) quorum sensing system is involved in the virulence of e. tarda. in the present study, it was found that the e. tarda luxs mutants bearing deletions of the catalytic site (c site) and the tyrosine kinase phosphorylation site, respectively, are functionally inactive and that these dysfunctional mutants can interfere with t ... | 2009 | 19411415 |
| immunoprotective analysis of vhhp2, a vibrio harveyi vaccine candidate. | vhhp2 is an outer membrane protein identified in a pathogenic vibrio harveyi strain, t4, isolated from diseased fish. when used as a subunit vaccine, purified recombinant vhhp2 affords high level of protection upon japanese flounder against v. harveyi challenge. vaccination with vhhp2 induced the expression of a number of immune-related genes, especially those encoding immunoglobulin m (igm) and major histocompatibility complex (mhc) iialpha. a vhhp2 surface display system, in the form of the fi ... | 2009 | 19428886 |
| identification, characterization, and molecular application of a virulence-associated autotransporter from a pathogenic pseudomonas fluorescens strain. | a gene, pfa1, encoding an autotransporter was cloned from a pathogenic pseudomonas fluorescens strain, tss, isolated from diseased fish. the expression of pfa1 is enhanced during infection and is regulated by growth phase and growth conditions. mutation of pfa1 significantly attenuates the overall bacterial virulence of tss and impairs the abilities of tss in biofilm production, interaction with host cells, modulation of host immune responses, and dissemination in host blood. the putative protei ... | 2009 | 19447960 |
| characterization of proteins secreted from a type iii secretion system of edwardsiella tarda and their roles in macrophage infection. | the type iii secretion system is essential for intracellular replication of edwardsiella tarda in phagocytes of fish and mammals. we identified the secreted proteins of the type iii secretion system by comparing the wild-type strain and the type iii mutant met1229. the wild-type strain secreted 55, 25, and 22 kda proteins into the culture supernatant, whereas the type iii mutant did not. these proteins were identified as eseb, esec, and esed and are similar in sequence to salmonella sseb, ssec, ... | 2009 | 19476281 |
| specific mhc class ii b alleles associated with resistance to edwardsiella tarda in turbot, psetta maxima (l.). | 2009 | 19486240 | |
| extraintestinal manifestations of edwardsiella tarda infection: a 10-year retrospective review. | edwardsiella tarda, a member of the family enterobacteriaceae found in aquatic environments, is an unusual cause of human disease, presenting most frequently as gastroenteritis. extraintestinal manifestations of e. tarda infection are rare but have included meningitis, cholecystitis, endocarditis, osteomyelitis, soft tissue infections, bacteremia, and septicemia. over a 10-year period at our institution, 10 cases of extraintestinal infection related to e. tarda were identified. the infections ra ... | 2009 | 19489391 |
| comparative analysis of the production of nitric oxide (no) and tumor necrosis factor-alpha (tnf-alpha) from macrophages exposed to high virulent and low virulent strains of edwardsiella tarda. | we previously reported that high virulent strain (nuf251) of edwardsiella tarda has an ability to prevent the production of reactive oxygen species by macrophages, and is even capable of surviving and multiplying within japanese flounder (paralichthys olivaceus) peritoneal macrophages, whereas the low virulent strain (nuf194) has no such ability. in this study, we found that nuf251 and nuf194 induced no and tnf-alpha production from japanese flounder peritoneal macrophages, and nuf251 caused fas ... | 2009 | 19539031 |
| detection of different quorum-sensing signal molecules in a virulent edwardsiella tarda strain ltb-4. | the aim of this study was to elucidate the potential quorum-sensing (qs) signal molecules of an emerging pathogen (edwardsiella tarda strain ltb-4) of cultured turbot (scophthalmus maximus). | 2010 | 19548884 |
| urosepsis caused by edwardsiella tarda. | edwardsiella tarda is a rare causative agent of human infection, predominantly associated with gastroenteritis. we describe a fatal case of urosepsis caused by e. tarda. the patient's underlying condition of advanced uterine cancer may have contributed to the development of the infection. | 2009 | 19554405 |
| edwardsiella tarda t6ss component evpp is regulated by esrb and iron, and plays essential roles in the invasion of fish. | edwardsiella tarda is a gram-negative pathogen for hemorrhagic septicemia in a broad range of hosts. the type vi secretion system (t6ss) has recently been dissected in e. tarda to secrete evpc, evpi and a novel effector protein evpp. in this study, sequencing and genetic alignments showed that evpp genes from different e. tarda isolates were highly similar and an evpp homolog was also found in aeromonas hydrophila 0865 isolated from a diseased eel, suggesting the possible lateral gene transfer o ... | 2009 | 19563898 |
| identification of major antigenic proteins of edwardsiella tarda recognized by japanese flounder antibody. | edwardsiella tarda is a fish pathogen that causes systemic infections in fresh water and marine fish. determining the antigenic proteins is important for the development of an immunodiagnostic tests and a vaccine for effective infection control in fish. in the current study, antigens were detected by immunoblotting and affinity column chromatography using a japanese flounder (paralichthys olivaceus) antibody produced by experimental infection with e. tarda. groel, glyceraldehyde-3-phosphate dehy ... | 2009 | 19564499 |
| identification and characterization of the acrr/acrab system of a pathogenic edwardsiella tarda strain. | edwardsiella tarda is one of the leading marine pathogens that can infect a wide range of cultured marine species. in this study, the acrr-acrab cluster was cloned from tx1, a pathogenic e. tarda strain isolated from diseased fish. acrr and acrab were found to be involved in resistance against acriflavine and methyl viologen, which positively regulate the expression of acrab. acrr negatively regulates its own expression and the expression of the acrab operon, most likely by interacting with a 24 ... | 2009 | 19590146 |
| construction and evaluation of dna vaccines encoding edwardsiella tarda antigens. | edwardsiella tarda is an opportunistic pathogen that can infect humans, animal, and fish. two e. tarda antigens, eta6 and flic, which are homologues to an ecotin precursor and the flic flagellin, respectively, were identified by in vivo-induced antigen technology from a pathogenic e. tarda strain isolated from diseased fish. when used as a subunit vaccine, purified recombinant eta6 was moderately protective against lethal challenge of e. tarda in a japanese flounder model, whereas purified recom ... | 2009 | 19596416 |
| genomic organization and mrna expression of manganese superoxide dismutase (mn-sod) from hemibarbus mylodon (teleostei, cypriniformes). | the genomic structure of manganese superoxide dismutase (mn-sod) was characterized in hemibarbus mylodon (teleostei, cypriniformes), an endangered freshwater fish species, and changes in mn-sod mrna expression in response to various stimuli, such as challenge with lipopolysaccharide (lps), bacteria, or heavy metals, were examined. h. mylodon mn-sod possesses well-conserved features of vertebrate mn-sods, including its homologous quinquepartite gene structure (five exons interrupted by four intro ... | 2009 | 19616630 |
| molecular cloning and characterization of lps-binding protein/bactericidal permeability-increasing protein (lbp/bpi) from olive flounder, paralichthys olivaceus. | a lipopolysaccharide (lps)-binding protein/bactericidal permeability-increasing protein (lbp/bpi) homolog was isolated from peripheral blood leukocytes cdna library of olive flounder paralichthys olivaceus. the isolated lbp/bpi cdna is 2806bp in length with a 1419bp open reading frame (orf) that encodes a protein of 472 amino acid residues. the lps-binding domain is well conserved in the n-terminal barrel, showing high sequence identities with other teleost lbp/bpi as well as those of mammals. r ... | 2010 | 19698997 |
| identification and immunoprotective analysis of an in vivo-induced edwardsiella tarda antigen. | edwardsiella tarda is a severe aquaculture pathogen that can infect many important fish species cultured worldwide. the aim of this study was to evaluate the vaccine potential of an e. tarda antigen, eta21, which was identified from a pathogenic e. tarda strain via the method of in vivo-induced antigen technology (iviat). eta21 is 510-amino acid in length and shares approximately 58% sequence identity with a putative peptidase of several bacterial species. eta21 was subcloned into escherichia co ... | 2009 | 19706328 |
| motility and the expression of the flagellin protein flic are negatively regulated by quorum sensing in edwardsiella tarda. | edwardsiella tarda is a gram-negative bacterial pathogen of fish and animals. a number of gram-negative bacteria have quorum-sensing systems and produce n-acylhomoserine lactone (ahl) that they use as a quorum-sensing signaling molecule. we have already reported that e. tarda nuf251 produces ahls and has the ahl-synthase gene, edwi. inactivation of nuf251 edwi induces expression of an approximately 45 kda extracellular protein, identified as a flagellin encoded by flic. mutation of edwi also cha ... | 2009 | 19716521 |
| regulation of the edwardsiella tarda hemolysin gene and luxs by ethr. | edwardsiella tarda is a pathogen with a broad host range that includes human and animals. the e. tarda hemolysin (eth) system, which comprises etha and ethb, is a noted virulence element that is widely distributed in pathogenic isolates of e. tarda. previous study has shown that the expression of ethb is regulated by iron, which suggests the possibility that the ferric uptake regulator (fur) is involved in the regulation of ethb. the work presented in this report supports the previous findings a ... | 2009 | 19734713 |
| isolation of faecal coliform bacteria from the american alligator (alligator mississippiensis). | to determine whether american alligators (alligator mississippiensis) are an unrecognized poikilothermic source of faecal coliform and/or potential pathogenic bacteria in south carolina's coastal waters. | 2010 | 19735329 |
| a novel c1q family member with fucose-binding activity from surfperch, neoditrema ransonnetii (perciformes, embiotocidae). | the c1q family is a growing group of proteins with a globular c1q domain in the c-terminal region. we purified a new member of this family with l-fucose-binding activity from the plasma of surfperch, neoditrema ransonnetii through l-fucose-affinity chromatography and anion-exchange chromatography. n-terminal amino acid sequencing followed by cdna sequencing revealed that the protein was composed of 212 amino acids including a signal peptide of 20 amino acids. the gene expression analysis by rt-p ... | 2009 | 19772923 |
| dna adenine methylase is involved in the pathogenesis of edwardsiella tarda. | edwardsiella tarda is a serious aquaculture pathogen that can infect many cultured fish species. the aim of this study was to investigate the potential importance of dna adenine methylase (dam) in e. tarda pathogenesis. the e. tarda dam gene (dam(et)) was cloned from a pathogenic strain, txd1, isolated from diseased fish. dam(et) shares high (70.2%) sequence identity with the dam proteins of yersinia enterocolitica and several other bacterial species. recombinant dam(et) is able to complement a ... | 2010 | 19781866 |
| genome sequence of the versatile fish pathogen edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. | edwardsiella tarda is the etiologic agent of edwardsiellosis, a devastating fish disease prevailing in worldwide aquaculture industries. here we describe the complete genome of e. tarda, eib202, a highly virulent and multi-drug resistant isolate in china. | 2009 | 19865481 |
| identification of edwardsiella ictaluri and e. tarda by species-specific polymerase chain reaction targeted to the upstream region of the fimbrial gene. | phylogenetic analysis of nine strains of edwardsiella ictaluri and eight strains of e. tarda (six typical motile strains and two atypical nonmotile strains) isolated from diseased fish was performed using the upstream region of the fimbrial gene cluster. strains of e. ictaluri and e. tarda were significantly clustered into separate groups. moreover, atypical e. tarda strains were clustered into a different group from the other strains. three polymerase chain reaction (pcr) primer sets for differ ... | 2009 | 19873834 |
| analysis of the effect of copper on the virulence of a pathogenic edwardsiella tarda strain. | to investigate the effect of copper on the virulence of edwardsiella tarda. | 2010 | 19912523 |
| domain analysis of the edwardsiella tarda ferric uptake regulator. | recent studies have shown that the ferric uptake regulator (fur) of edwardsiella tarda (fur(et)) shares high sequence identity with the escherichia coli fur (fur(ec)) at the n-terminal dna-binding region. in the present study, the functional importance of the c-terminal region of fur(et) was investigated. it was found that fur(et) bearing deletion of the c-terminal 12 residues still possesses most of the repressor activity, whereas fur(et) bearing deletions of the c-terminal 16 and more than 16 ... | 2009 | 19940381 |
| immune response of japanese flounder paralichthys olivaceus to outer membrane protein of edwardsiella tarda. | the humoral and cellular immune responses of japanese flounder, paralichthys olivaceus, were investigated following intraperitioneal injection with outer membrane protein (omp) of edwardsiella tarda in freund's incomplete adjuvant (fia). the specific serum antibody titre against omp of e. tarda were measured using elisa for 14 weeks, and the total serum antibody concentrations were also determined according to the sandwich elisa standard model constructed using purified igm. both of the specific ... | 2010 | 19944169 |
| transgenic zebrafish eggs containing bactericidal peptide is a novel food supplement enhancing resistance to pathogenic infection of fish. | zebrafish (danio rerio) was used as a bioreactor to produce bovine lactoferricin (lfb), which has wide-ranging antimicrobial activity. we constructed an expression plasmid in which lfb was fused with green fluorescent protein (gfp) and driven by zebrafish beta-actin promoter. after microinjection, six transgenic founders were screened on the basis of gfp appearance. among them, a stable zbl-5 line was selected by the ubiquitous and strong expression of gfp. using pcr and western blot analysis, w ... | 2010 | 19944764 |
| verification of an edwardsiella ictaluri-specific diagnostic pcr. | to verify the specificity of a pcr assay for the identification and diagnosis of edwardsiella ictaluri. | 2010 | 20002568 |
| proteomic analysis of outer membrane proteins of edwardsiella tarda. | the purpose of this study was to identify outer membrane proteins (omps) of edwardsiella tarda. | 2010 | 20002913 |
| comparative study of the effects of aluminum adjuvants and freund's incomplete adjuvant on the immune response to an edwardsiella tarda major antigen. | edwardsiella tarda is a severe aquaculture pathogen that can infect many different fish species cultured worldwide. et49 is a major e. tarda antigen with weak immunoprotective potential. in this study, using et49 as an example vaccine, the adjuvanticity of freund's incomplete adjuvant (fia), aluminum hydroxide, and aluminum phosphate adjuvant were evaluated in a japanese flounder model. the results showed that the presence of fia, aluminum hydroxide, and aluminum phosphate adjuvant increased the ... | 2010 | 20006568 |
| immune responses and expression profiles of some immune-related genes in indian major carp, labeo rohita to edwardsiella tarda infection. | edwardsiella tarda is an important gram-negative bacterium that causes systemic infections in a wide range of hosts including fish. the pathogenic mechanisms in this disease are still poorly understood in fish. indian major carp, labeo rohita were intraperitoneally challenged with a pathogenic isolate of e. tarda to measure sequential changes in immunity level. a significant decrease in the superoxide production, myeloperoxidase, alternative complement activity, total protein levels and antiprot ... | 2010 | 20045061 |
| identification and characterization of matrix metalloproteinase-13 sequence structure and expression during embryogenesis and infection in channel catfish (ictalurus punctatus). | matrix metalloproteinase-13 (mmp-13), referred to as collagenase-3, is a proteolytic enzyme that plays a key role in degradation and remodelling of host extracellular matrix proteins. the objective of this study was to characterize the mmp-13 gene in channel catfish, and to determine its pattern of expression in various healthy tissues and during embryogenesis. since mmp-13 has been shown to have importance in tissue remodelling and some pathological processes, we further studied its involvement ... | 2010 | 20060415 |
| analysis of edwardsiella tarda degp, a serine protease and a protective immunogen. | edwardsiella tarda is a severe aquaculture pathogen with a broad host range that includes humans, animal, and fish. a gene (degp(et)) encoding a degp homologue was cloned from tx01, a pathogenic e. tarda strain isolated from diseased fish. degp(et) shares high sequence identities with the degp proteins of several bacterial species. functional analyses showed that degp(et) could complement the temperature-sensitive phenotype of an escherichia coli degp null mutant. expression of degp(et) in tx01 ... | 2010 | 20060910 |
| esed, a putative t3ss translocon component of edwardsiella tarda, contributes to virulence in fish and is a candidate for vaccine development. | edwardsiella tarda has a type iii secretion system (t3ss) essential for pathogenesis. esed, together with eseb and esec, has been suggested to form a putative t3ss translocon complex, although its further function is unclear. to investigate the physiological role of esed, a mutant strain of e. tarda was constructed with an in-frame deletion of the entire esed gene. one finding was that the ∆esed mutant decreased the secretion levels of esec and eseb proteins. additionally, the ∆esed mutant showe ... | 2010 | 20072793 |
| analysis of the vaccine potential of a natural avirulent edwardsiella tarda isolate. | edwardsiella tarda is a severe aquaculture pathogen that can infect many fish species and cause a systematic disease called edwardsiellosis, which can lead to high mortality under certain conditions. currently, most vaccine candidates against edwardsiellosis are based on pathogenic e. tarda strains, which can be a concern in some cases. in this study, the vaccine potential of a natural e. tarda isolate, atcc 15947, was examined in a japanese flounder model. atcc 15947 was found to be relatively ... | 2010 | 20109592 |
| production of an anti-idiotypic antibody single chain variable fragment vaccine against edwardsiella tarda. | edwardsiella tarda is the pathogen responsible for edwardsiellosis, a serious infectious disease of freshwater and marine fish species, and currently recognized to be the species pathogenic for human. an anti-idiotypic monoclonal antibody (mab), 1e11, has been developed. it mimics the protective epitope of e. tarda and can prevent fish from infection of e. tarda. in this study, the correct variable heavy (vh) and variable light (vl) genes were obtained from 1e11 by using bioinformatics methods, ... | 2010 | 20119624 |
| structural characteristics and antioxidant activities of the extracellular polysaccharides produced by marine bacterium edwardsiella tarda. | two water-soluble extracellular polysaccharides, etw1 and etw2, were isolated from the marine bacterium edwardsiella tarda by ion-exchange and size-exclusion chromatography, and their structures were investigated. etw1 and etw2 are mannans, with molecular weights of about 29 and 70kda, respectively. the main chain of the polysaccharides consists of (1-->3)-linked mannose residues, and branched mannose linkages were also detected. the branch points are located at the c-2 and c-6 positions of the ... | 2010 | 20156684 |
| genetic tools for tagging gram-negative bacteria with mcherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies. | live-cell imaging techniques are essential to gain a better understanding of microbial functioning in natural systems, for example in biofilms. autofluorescent proteins, such as the green fluorescent protein (gfp) and the red fluorescent protein (dsred), are valuable tools for studying microbial communities in their natural environment. because of the functional limitations of dsred such as slow maturation and low photostability, new and improved variants were created such as mcherry. in this st ... | 2010 | 20180857 |
| identification and molecular analysis of a novel c-type lectin from scophthalmus maximus. | c-type lectins are calcium-dependent carbohydrate-binding proteins that play important roles in innate immunity. in this study, a c-type lectin homologue (smlec1) was identified from turbot (scophthalmus maximus) and analyzed at expression and functional levels. the open reading frame of smlec1 is 504 bp, with a 5'-untranslated region (utr) of 101 bp and a 3'-utr of 164 bp. the deduced amino acid sequence of smlec1 shares 34%-38% overall identities with the c-type lectins of several fish species ... | 2010 | 20226863 |
| protection of red sea bream pagrus major against red sea bream iridovirus infection by vaccination with a recombinant viral protein. | megalocytivirus infections cause serious mass mortality in marine fish in east and southeast asian countries. in this study the immunogenicity of crude subunit vaccines against infection by the megalocytivirus rsiv was investigated. three capsid proteins, 18r, 351r and a major capsid protein, were selected for use as crude subunit vaccines. high homology among megalocytivirus types was found in the initial sequence examined, the 351r region. red sea bream (pagrus major) juveniles were vaccinated ... | 2010 | 20236423 |
| identification and characterization of japanese flounder, paralichthys olivaceus interferon-stimulated gene 15 (jf-isg15). | previously, using cdna microarray analysis, we demonstrated that an est clone of japanese flounder (paralichthys olivaceus) with homology to mammalian interferon-stimulated gene 15 (isg15) was strongly induced by treatment with dna vaccine encoding the glycoprotein gene of hirame rhabdovirus (hirrv). in this study, we conducted molecular cloning and expression analysis of the japanese flounder isg15 (jf-isg15). jf-isg15 encoded two exons. the first exon was non-coding, while the second exon enco ... | 2011 | 20299096 |
| broad-host-range plasmids for red fluorescent protein labeling of gram-negative bacteria for use in the zebrafish model system. | to observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (danio rerio), a series of plasmids was constructed for the red fluorescent protein (rfp) labeling of a variety of fish and human pathogens. the aim of this study was to create a collection of plasmids that would express rfp pigments both constitutively and under tac promoter regulation and that would be nontoxic and broadly transmissible to a variety of gram-negative b ... | 2010 | 20363780 |
| novel expression system for combined vaccine production in edwardsiella tarda ghost and cadaver cells. | to develop combined vaccine systems, we have generated edwardsiella tarda ghosts (etg) displaying a foreign protein on the outer membrane and also ed. tarda cadaver (etc) expressing a heterologous protein in the cytoplasm. green fluorescent protein (gfp) was used as a model foreign protein. a constitutive promoter (etpr c28-1) cloned newly from ed. tarda was used as a promoter for the expression of foreign protein. comparison of the strength of the new promoter with a commercially available cons ... | 2010 | 20369310 |
| identification and analysis of a sciaenops ocellatus isg15 homologue that is involved in host immune defense against bacterial infection. | isg15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. isg15 homologues have been identified in a number of fish species, some of which are known to be regulated at expression level by virus infection and lipopolysaccharide (lps) treatment. however, the relationship between isg15 and live bacterial infection has not been investigated in piscine models. in this study, an isg15 homologue, soisg15, was identified from red drum sciaenops ocellatus and analyzed at expression an ... | 2010 | 20385242 |
| differential regulation of sciaenops ocellatus viperin expression by intracellular and extracellular bacterial pathogens. | viperin is an antiviral protein that has been found to exist in diverse vertebrate organisms and is involved in innate immunity against the infection of a wide range of viruses. however, it is largely unclear as to the potential role played by viperin in bacterial infection. in this study, we identified the red drum sciaenops ocellatus viperin gene (sovip) and analyzed its expression in relation to bacterial challenge. the complete gene of sovip is 2570 bp in length and contains six exons and fi ... | 2010 | 20420911 |
| defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions. | a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ... | 2010 | 20537393 |
| molecular cloning and expression study on toll-like receptor 5 paralogs in japanese flounder, paralichthys olivaceus. | toll-like receptor (tlr) 5 is responsible for the bacterial flagellin recognition in vertebrates. synergistic role of tlr 5 membrane form (tlr 5m) and tlr 5 soluble form (tlr 5s) have been reported from the study on rainbow trout. this system is regarded as the unique system in teleost fish. however, systemic response of tlr 5 genes in teleost fish has not been fully understood. hence, we cloned japanese flounder (paralichthys olivaseus) tlr 5m and tlr 5s genes and their expressions were analyze ... | 2010 | 20561590 |
| dissection and localization of the immunostimulating domain of edwardsiella tarda flic. | bacterial flagellin is known to induce potent immune response in vertebrate systems via the toll-like receptor (tlr) 5. as a result, flagellin has been studied extensively as a vaccine adjuvant. in a previous study, we examined the vaccine and adjuvant potentials of the flagellin (flic) of the fish pathogen edwardsiella tarda. we found that e. tarda flic induced low protective immunity by itself but could function as a molecular adjuvant and potentiate the specific immune response induced by the ... | 2010 | 20580470 |
| evaluation of four polymerase chain reaction primer pairs for the detection of edwardsiella tarda in turbot. | edwardsiella tarda is an important emergent pathogen in european aquaculture, causing several mortality events in turbot scophthalmus maximus cultures in recent years. here, we evaluated in parallel the specificity of 4 previously published pairs of primers, gyrbf1/gyrbr1, tardaf/ tardar, etfa and etfd, for the detection of 53 e. tarda strains isolated from different sources, as well as 18 representatives of related and unrelated bacterial species. on the basis of the obtained results, we select ... | 2010 | 20597430 |
| smc2p1, a c2 domain protein from scophthalmus maximus that binds bacterial pathogen-infected lymphocytes and reduces bacterial survival. | c2 domains are protein structural modules found in many eukaryotic proteins involved in signal transduction, membrane trafficking, and immune defense. most of the studied c2 domain-containing proteins are multi-domained in structure, in which the c2 domain is an independently folded motif and plays an essential role in calcium-dependent membrane-targeting. although c2 domains isolated from intact proteins have been studied for biological functions, no study on natural proteins containing c2 doma ... | 2010 | 20633652 |
| extracellular products from virulent strain of edwardsiella tarda stimulate mouse macrophages (raw264.7) to produce nitric oxide (no) and tumor necrosis factor (tnf)-alpha. | we have previously found that high virulent strain (nuf251) of edwardsiella tarda, but not low virulent strain (nuf194), was able to survive and multiply within japanese flounder (paralichthys olivaceus) peritoneal macrophages. further studies demonstrated that nuf251 induced much higher levels of no and tnf-alpha productions than nuf194 in both japanese flounder peritoneal macrophages and mouse macrophage cell line raw264.7. in this study, we examined the effects of extracellular products (ecp) ... | 2010 | 20633653 |
| the efficacy of five avirulent edwardsiella tarda strains in a live vaccine against edwardsiellosis in japanese flounder, paralichthys olivaceus. | we evaluated the tissue persistence and live vaccine efficacy of five avirulent edwardsiella tarda strains (e22, su100, su117, su138, and su244) isolated from the japanese eel (anguilla japonica) and from the environment. the live vaccines, containing a single strain, were injected intraperitoneally into japanese flounder (paralichthys olivaceus). viable bacteria from all the strains (excluding su100) were recovered from trunk-kidney tissue 28 d post-injection. japanese flounder inoculated with ... | 2010 | 20633656 |
| differential gene expression profiles in japanese flounder (paralichthys olivaceus) with different susceptibilities to edwardsiellosis. | edwardsiellosis, which is caused by edwardsiella tarda, a gram-negative bacterium, is one of the most serious problems in japanese flounder (paralichthys olivaceus) culture. in this study, we examined the immune responses at the molecular level of genetic groups of japanese flounder that are either susceptible or moderately susceptible to e. tarda infection using a cdna microarray which was spotted with approximately 2000 different genes. four different genetic groups of japanese flounder (group ... | 2010 | 20637288 |
| isolation and analysis of the vaccine potential of an attenuated edwardsiella tarda strain. | edwardsiella tarda is an important aquaculture pathogen that can infect a wide range of marine and freshwater fish worldwide. in this study, a modified e. tarda strain, tx5rm, was selected by multiple passages of the pathogenic e. tarda strain tx5 on growth medium containing the antibiotic rifampicin. compared to the wild type strain, the rifampicin-resistant mutant tx5rm (i) shows drastically increased median lethal dose and reduced capacity to disseminate in and colonize fish tissues and blood ... | 2010 | 20637307 |
| identification of an edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain. | edwardsiella tarda is the etiological agent of edwardsiellosis, a systematic disease that affects a wide range of marine and freshwater fish cultured worldwide. in order to identify e. tarda antigens with vaccine potential, we in this study conducted a systematic search for e. tarda proteins with secretion capacity. one of the proteins thus identified was esa1, which contains 795 amino acid residues and shares extensive overall sequence identities with the d15-like surface antigens of several ba ... | 2010 | 20673823 |
| microarray technology is an effective tool for identifying genes related to the aquacultural improvement of japanese flounder, paralichthys olivaceus. | molecular techniques are now essential for discovering new functional genes for the improvement of cultured marine organisms. such techniques can be used to obtain expressed sequence tags (est) and, through the use of microarrays, the entire transcriptome. for example, microarrays can be used to reveal biomarkers of health conditions, nutrient changes and immune response in fish and shellfish. est-based microarray chips were constructed for cultured fish and shellfish species including japanese ... | 2011 | 20685186 |
| scophthalmus maximus cystatin b enhances head kidney macrophage-mediated bacterial killing. | cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. however, much less is known about fish cystatins. in this report, we described the identification and analysis of a cystatin b homologue, smcytb, from turbot scophthalmus maximus. the open reading frame of smcytb is 300bp, which encodes a 99-residue protein that shares high le ... | 2010 | 20692286 |
| characterization of edwardsiella tarda isolated from farm-cultured eels, anguilla japonica, in the republic of korea. | we surveyed the occurrence of edwardsiellosis on eel farms and investigated the characteristics of edwardsiella tarda isolated from farm-cultured eels in the republic of korea. the occurrence rate of edwardsiellosis was 72% in the investigated samples. among the edwardsiellosis cases, 46% were found to be mixed infections, with parasites and other kinds of bacteria. some of the biochemical characteristics of the e. tarda isolates were different from those of the previously reported e. tarda isol ... | 2011 | 20716861 |
| molecular cloning and characterization of toll-like receptor 14 in japanese flounder, paralichthys olivaceus. | toll-like receptors (tlrs) are essential for activation of the innate immune system in response to invading pathogens. tlr14, which is unique to fish, has been identified in several fish species, but its function is unclear. in this study, japanese flounder (paralichthys olivaceus) tlr14 gene (jftlr14) was cloned and its expression profiles were analyzed after infection with viral hemorrhagic septicemia virus, gram-positive streptococcus iniae and gram-negative edwardsiella tarda. the coding reg ... | 2011 | 20728538 |
| the iron-cofactored superoxide dismutase of edwardsiella tarda inhibits macrophage-mediated innate immune response. | edwardsiella tarda is a gram-negative bacterium that can infect a wide variety of marine and freshwater fish and cause severe economic losses worldwide. with an aim to elucidate the virulence mechanism of e. tarda, we in this study cloned and analyzed the function of an iron-cofactored superoxide dismutase (fesod) from a pathogenic e. tarda strain tx01 isolated from diseased fish. fesod is 192-residue in length and contains domain structures that are conserved among iron/manganese superoxide dis ... | 2010 | 20732430 |
| neonatal sepsis following maternal amnionitis by edwardsiella tarda: a case report and a review of the literature. | edwardsiella tarda, a gram-negative bacterium, is a rare pathogen in the neonatal period. we present a term newborn that developed e. tarda septicemia following maternal amnionitis. the severe neurological outcome in this case, as well as in all other reported cases, highlights the need for meticulous neurological evaluation in neonates presenting with e. tarda septicemia even in the absence of bona fide meningitis. | 2011 | 20827557 |
| hemolysin etha in edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system esra-esrb and nucleoid protein hhaet. | edwardsiella tarda is a gram-negative pathogen for hemorrhagic septicemia in fish. recently, two-component system (tcs) esra-esrb in e. tarda has been found to play key roles in regulating type iii secretion system (ttss) and type vi secretion system (t6ss). in this study, a markedly attenuated δesrb mutant was investigated to exhibit enhanced cell-invasion capability, as well as the increased cytotoxicity of its extracellular products (ecps). compared with the parental strain, the δesrb mutant ... | 2010 | 20832475 |
| eseg, an effector of the type iii secretion system of edwardsiella tarda, triggers microtubule destabilization. | edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and both gastrointestinal and extraintestinal infections in humans. a type iii secretion system (t3ss) was recently shown to contribute to pathogenesis, since deletions of various t3ss genes increased the 50% lethal dose (ld(50)) by about 1 log unit in the blue gourami infection model. in this study, we report eseg as the first identified effector protein of t3ss. eseg shares partial homology with t ... | 2010 | 20855515 |
| molecular cloning and expression analysis of interferon regulatory factor 10 (irf10) in japanese flounder, paralichthys olivaceus. | interferons (ifns) are important for the defense against intracellular pathogens. interferon regulatory factor 10 (irf10) is a transcript factor involved in regulating ifn signaling induced by virus infections in birds, but little is know of its role in the immune response in non-avian vertebrates. here, we identified and characterized the irf10 gene and examined its expression pattern in a teleost fish, japanese flounder, paralichthys olivaceus. japanese flounder irf10 (poirf10) gene is a singl ... | 2010 | 20883793 |