Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| identification and characterization of aarf, a locus required for production of ubiquinone in providencia stuartii and escherichia coli and for expression of 2'-n-acetyltransferase in p. stuartii. | providencia stuartii contains a chromosomal 2'-n-acetyltransferase [aac(2')-ia] involved in the o acetylation of peptidoglycan. the aac(2')-ia enzyme is also capable of acetylating and inactivating certain aminoglycosides and confers high-level resistance to these antibiotics when overexpressed. we report the identification of a locus in p. stuartii, designated aarf, that is required for the expression of aac(2')-ia. northern (rna) analysis demonstrated that aac(2')-ia mrna levels were dramatica ... | 1998 | 9422602 |
| nitrate-reducing bacteria in diversion colitis: a clue to inflammation? | a pathogenic role of nitric oxide has been suggested in acute and chronic intestinal inflammation. we took the opportunity offered by studies in patients with excluded colon, which represents a model of chronic intestinal inflammation with no exogenous nitrite or nitrate supply, to evaluate the quantity and the quality of nitrate reducers in diversion colitis. thirty patients (17 men, 13 women, mean age 45 years) having an excluded colon for various reasons were sampled by rectal swabs and compa ... | 1997 | 9440640 |
| [urinary bacteria in elderly patients with urinary incontinence and low levels of daily activity]. | the results of bacteria isolated from the urine of inactive elderly patients with urinary incontinence in 1982, 87, 93 and 95 were compared and the chronological changes of urinary bacteria were analyzed. the following results were obtained: (1) the most common bacteria isolated from the patients in 1995 were d-group enterococcus, followed by providencia rettgeri, e. coli, pseudomonas aeruginosa and proteus mirabilis. it is thought that the main cause of the more frequent isolation of enterococc ... | 1997 | 9493467 |
| flies and their bacterial loads in greyhound dog kennels in kansas. | breeders of greyhound dogs traditionally feed racing animals and nursing bitches raw meat, and that meat generally is obtained frozen from commercial renderers. previous studies have shown that the rendered meat is frequently contaminated with enteric bacteria, including salmonella spp., and that during thawing the rendered meat is exposed to filth flies common in dog kennels. nursing greyhound pups tend to experience a high morbidity and mortality from intestinal infections, and we attempted to ... | 1998 | 9516545 |
| mutations in aare, the ubia homolog of providencia stuartii, result in high-level aminoglycoside resistance and reduced expression of the chromosomal aminoglycoside 2'-n-acetyltransferase. | the aare1 allele was identified on the basis of the resulting phenotype of increased aminoglycoside resistance. the aare1 mutation also resulted in a small-colony phenotype and decreased levels of aac(2')-ia mrna. the deduced aare gene product displayed 61% amino acid identity to the escherichia coli ubia protein, an octaprenyltransferase required for the second step of ubiquinone biosynthesis. complementation experiments in both providencia stuartii and e. coli demonstrated that aare and ubia a ... | 1998 | 9559821 |
| association of providencia alcalifaciens with diarrhea in children. | it has been demonstrated in previous studies that providencia alcalifaciens can produce diarrhea by an invasive mechanism. in the present study, p. alcalifaciens was isolated from the stool specimens of 17 of 814 diarrheal children younger than 5 years of age (2.1%) and from those of 4 of 814 matched controls (0.49%) (p = 0.004), indicating that the organism is significantly associated with diarrhea. however, 71% of p. alcalifaciens-positive diarrheal children had simultaneous infections with ot ... | 1998 | 9574723 |
| antimicrobial spectrum and target site of d-3-phenyllactic acid. | geotrichum candidum excretes d-3-phenyllactic acid, which inhibits the growth of listeria monocytogenes. it was found to inhibit a range of gram-positive bacteria found in humans and foodstuffs, such as staphylococcus aureus and enterococcus faecalis, and gram-negative bacteria from humans, such as providencia stuartii and klebsiella oxytoca. scanning electron microscope studies on the effect of d-3-phenyllactic acid on l. monocytogenes showed that it caused changes in bacterial behavior and str ... | 1998 | 9620125 |
| ceftazidime and aztreonam resistance in providencia stuartii: characterization of a natural tem-derived extended-spectrum beta-lactamase, tem-60. | a plasmid-encoded beta-lactamase produced from a clinical strain of providencia stuartii has been purified and characterized. the gene coding for the beta-lactamase was cloned and sequenced. it appears to be a new natural tem-derived enzyme, named tem-60. point mutations (q39k, l51p, e104k, and r164s) are present with respect to the tem-1 enzyme; the mutation l51p has never been previously reported, with the exception of the chromosomally encoded extended-spectrum beta-lactamase per-1. kinetic p ... | 1998 | 9624494 |
| men 10700, a new penem antibiotic: in-vitro activity and its correlation with beta-lactamase stability, pbp affinity and diffusion through the bacterial cell wall. | the in-vitro activity of men 10700, a novel penem, was compared with that of imipenem, ritipenem, ampicillin/sulbactam, cefotaxime, ciprofloxacin and amikacin against 1088 strains taken from 21 genera, including gram-negative, gram-positive and anaerobic bacteria. mic data showed that men 10700 was very active against staphylococci and streptococci (mic90 < or = 0.5 mg/l) and against most members of the enterobacteriaceae (mic90 < or = 2 mg/l), with reduced activity only against providencia stua ... | 1998 | 9630405 |
| important and emerging beta-lactamase-mediated resistances in hospital-based pathogens: the amp c enzymes. | resistance to third-generation cephalosporins mediated by beta-lactamases is an increasing problem for clinical therapeutics. a wide range of enterobacteriaceae produce these ampc enzymes (bush-jacoby-medeiros group 1), including enterobacter spp., citrobacter freundii, morganella morganii, providencia spp., and serratia marcescens. resistance via this mechanism has been shown to be statistically correlated with the use of some third-generation cephalosporins, and the infections caused by these ... | 1998 | 9635237 |
| recurrent bilateral renal calculi in a tetraplegic patient. | an 18-year-old male developed c-5 complete tetraplegia following a motor-cycle accident in may 1975. the neuropathic bladder was managed by an indwelling urethral catheter. he developed recurrent episodes of urinary infection with proteus species. in september 1975, an x-ray of the abdomen revealed small calculi in both the kidneys. in july 1976, he underwent transurethral resection of the bladder neck and division of the external urethral sphincter; subsequently, he was put on a penile sheath d ... | 1998 | 9670380 |
| a regulatory cascade involving aarg, a putative sensor kinase, controls the expression of the 2'-n-acetyltransferase and an intrinsic multiple antibiotic resistance (mar) response in providencia stuartii. | a recessive mutation, aarg1, has been identified that resulted in an 18-fold increase in the expression of beta-galactosidase from an aac(2')-lacz fusion. transcriptional fusions and northern blot analysis demonstrated that the aarg1 allele also resulted in a large increase in the expression of aarp, a gene encoding a transcriptional activator of aac(2')-ia. the effects of aarg1 on aac(2')-ia expression were mediated by aarp-dependent and -independent mechanisms. the aarg1 allele also resulted i ... | 1998 | 9680222 |
| invasion of hep-2 and other eukaryotic cell lines by providenciae: further evidence supporting the role of providencia alcalifaciens in bacterial gastroenteritis. | wild-type strains of providencia species were evaluated for their ability to invade hep-2 monolayers based upon microscopic and semi-quantitative assays. of 14 p. alcalifaciens strains tested, 3 (17%) were found to be highly invasive, 4 (22%) moderately invasive, and the remaining 61% weakly or noninvasive. hep-2 invasion results were confirmed by thin-section electron microscopy. invasive capabilities of p. alcalifaciens were greater at higher mois (100 to 1000) than at lower inocula (<10 moi). ... | 1998 | 9688814 |
| identification of n epsilon-[(r)-1-carboxyethyl]-l-lysine in, and the complete structure of, the repeating unit of the o-specific polysaccharide of providencia alcalifaciens o23. | n epsilon-[(r)-1-carboxyethyl]-l-lysine was released by acid hydrolysis from the o-specific polysaccharide of providencia alcalifaciens o23 and identified by 1h and 13c nmr spectroscopy, glc-ms after conversion to a di-n-acetylated dimethyl ester, and by comparison with the authentic sample. solvolysis of the polysaccharide with anhydrous hf resulted in an amide of d-glucuronic acid with n epsilon-[(r)-1-carboxyethyl]-l-lysine. these and published data allowed the determination of the full struc ... | 1998 | 9720244 |
| gyra mutations associated with fluoroquinolone resistance in eight species of enterobacteriaceae. | fluoroquinolone resistance (fq-r) in clinical isolates of enterobacteriaceae species has been reported with increasing frequency in recent years. two mechanisms of fq-r have been identified in gram-negative organisms: mutations in dna gyrase and reduced intracellular drug accumulation. a single point mutation in gyra has been shown to reduce susceptibility to fluoroquinolones. to determine the extent of gyra mutations associated with fq-r in enteric bacteria, one set of oligonucleotide primers w ... | 1998 | 9756773 |
| [bactericidal activity and capacity for selection of mutants resistant to imipenmen and meropenem in strains of proteus, morganella and providencia]. | to compare the bactericidal activity and frequency of mutation for meropenem and imipenem against proteus mirabilis, morganella morganii and providencia rettgeri. | 1998 | 9763741 |
| gentamicin resistance in clinical strains of enterobacteriaceae associated with reduced gentamicin uptake. | seven strains of enterobacteriaceae resistant to gentamicin obtained as representatives of the predominant resistance profiles in the clinical laboratories of rafeidia and al-watani hospitals in nablus (palestine) were included. five strains showed a broad aminoglycoside resistance profile but contained no evidence of gentamicin acetylation, adenylation, or phosphorylation. gentamicin uptake in two tested strains was significantly reduced, compared to that of gentamicin-sensitive e. coli (mic, 0 ... | 1998 | 9821296 |
| [comparative study of morganella and providencia bacteriophages]. | 7 strains of m.morganii phages and 7 strains of p.rettgeri phages were isolated from lysogenic cultures and the environment. the main biological properties of these phages were studied. the phages under study formed independent taxonomic groups. these phages were found to be highly specific and capable of being used for the identification of bacteria. | 1998 | 9825491 |
| studies on the formation of crystalline bacterial biofilms on urethral catheters. | a model of the catheterised bladder was used to test the ability of urease-producing urinary tract pathogens to encrust urethral catheters. encrustation was assessed by determining the amounts of calcium and magnesium deposited on the catheters and visualised by scanning electron microscopy. urease-positive morganella morganii, klebsiella pneumoniae, and pseudomonas aeruginosa failed to raise the urinary ph and form crystalline biofilms. in contrast, strains of proteus mirabilis, proteus vulgari ... | 1998 | 9832268 |
| low-level colonization and infection with ciprofloxacin-resistant gram-negative bacilli in a skilled nursing facility. | we report a 1-year surveillance study that evaluates colonization and infection with ciprofloxacin-resistant gram-negative bacilli (cr gnb) and the relation to quinolone use and other possible risk factors in a proprietary skilled nursing facility (snf) with no history of outbreaks. | 1998 | 9836837 |
| natural antibiotic susceptibility of providencia stuartii, p. rettgeri, p. alcalifaciens and p. rustigianii strains. | the natural antibiotic susceptibility of 38 providencia rettgeri, 35 p. stuartii, 23 p. alcalifaciens and 20 p. rustigianii strains was examined. mic values were determined by a microdilution procedure and evaluated by a table calculation programme. p. stuartii was the least susceptible providencia sp. and was naturally resistant to tetracyclines, some penicillins, older cephalosporins, sulphamethoxazole and fosfomycin and to antibiotics to which other species of enterobacteriaceae are also resi ... | 1998 | 9839568 |
| interference in initial adhesion of uropathogenic bacteria and yeasts to silicone rubber by a lactobacillus acidophilus biosurfactant. | the ability of the lactobacillus acidophilus rc14 biosurfactant 'surlactin' to inhibit the initial adhesion of various uropathogenic bacteria and two yeast strains to silicone rubber was investigated in a parallel-plate flow chamber in filter-sterilised pooled human urine. a parallel-plate flow chamber with a silicone rubber bottom plate was filled with a 1.0 mg/ml biosurfactant solution for adsorption overnight (18 h). subsequently, the adhesion of the bacterial or yeast cells from a urine susp ... | 1998 | 9856644 |
| the chromosomal 2'-n-acetyltransferase of providencia stuartii: physiological functions and genetic regulation. | intrinsic chromosomal acetyltransferases involved in aminoglycoside resistance have been identified in a number of bacteria. in providencia stuartii, a chromosomal acetyltransferase (aac(2')-ia) has been characterized in detail. in addition to the ability to acetylate aminoglycosides, the aac(2')-ia enzyme has at least one physiological function, which is the acetylation of peptidoglycan. this modification is likely to influence the autolytic system in p. stuartii. the regulation of aac(2')-ia e ... | 1999 | 9924143 |
| clonal structure of providencia alcalifaciens strains isolated from diarrhoeal stools in são paulo, brazil. | clonal analysis based on ribotyping demonstrated that providencia alcalifaciens strains isolated mainly from diarrhoeal stools in são paulo, brazil, were clustered into two main groups. eleven distinct ribotype patterns were identified with clai, ecorv and mlui restriction endonucleases. p. alcalifaciens strains with invasive properties were of two ribotype patterns that differed from those identified among non-invasive strains. the ribotyping results confirmed that p. alcalifaciens strains asso ... | 1999 | 9989649 |
| synthesis and secretion of providencia rettgeri and escherichia coli heterodimeric penicillin amidases in saccharomyces cerevisiae. | the providencia rettgeri and escherichia coli pac genes encoding heterodimeric penicillin g amidases (pac) were successfully expressed in saccharomyces cerevisiae. furthermore, these recombinant enzymes are secreted from the yeast cell into the medium which is in contrast to bacterial hosts, where the enzymes are retained in the periplasm. contrary to the p. rettgeri pac-encoding gene, the e. coli pac is poorly expressed in yeast. the highest yield of p. rettgeri pac was obtained with a multi-co ... | 1999 | 10072775 |
| microbiology of the oil fly, helaeomyia petrolei. | helaeomyia petrolei larvae isolated from the asphalt seeps of rancho la brea in los angeles, calif., were examined for microbial gut contents. standard counts on luria-bertani, macconkey, and blood agar plates indicated ca. 2 x 10(5) heterotrophic bacteria per larva. the culturable bacteria represented 15 to 20% of the total population as determined by acridine orange staining. the gut itself contained large amounts of the oil, had no observable ceca, and maintained a slightly acidic ph of 6.3 t ... | 1999 | 10103240 |
| structure of a neutral o-specific polysaccharide of the bacterium providencia alcalifaciens o5. | a neutral polysaccharide containing d-galactose, 2-acetamido-2-deoxy-d-glucose, and 3-acetamido-3,6-dideoxy-d-glucose (qui3nac) in the ratios 2:1:1 was obtained by mild acid degradation of lipopolysaccharide of the bacterium providencia alcalifaciens o5 followed by gel chromatography and ion-exchange chromatography or treatment with anhydrous hydrogen fluoride. on the basis of full acid hydrolysis, methylation, and 1h- and 13c-nmr spectroscopy, including two-dimensional correlation spectroscopy ... | 1999 | 10381612 |
| activation of the 2'-n-acetyltransferase gene [aac(2')-ia] in providencia stuartii by an interaction of aarp with the promoter region. | the aac(2')-ia gene in providencia stuartii encodes a 2'-n-acetyltransferase capable of acetylating both peptidoglycan and certain aminoglycoside antibiotics. regulation of the aac(2')-ia gene is influenced in a positive manner by the product of the aarp gene, which encodes a small transcriptional activator of the arac (xyls) family. in this study, we demonstrate the sequence requirements at the aac(2')-ia promoter for aarp binding and activation. | 1999 | 10390241 |
| prevalence of proteus species in urinary tract infections in a regional hospital in trinidad. | proteus bacteria are a well-known cause of urinary tract infections (utis). the prevalence of utis is high among catheterized patients and those undergoing manipulation of the urinary tract. this study assessed the prevalence of utis due to proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital. | 1999 | 10418178 |
| tem-24 produced by four different species of enterobacteriaceae, including providencia rettgeri, in a single patient. | four species of members of the family enterobacteriaceae harboring extended-spectrum beta-lactamase (esbl) were recovered in a single patient hospitalized in an intensive care unit. among these isolates, we describe for the first time an esbl-producing providencia rettgeri strain. bacteria from the same species were shown to be genetically related by pulsed-field gel electrophoresis analysis. these strains produced the same tem derivative esbl, characterized as tem-24. this enzyme had the peculi ... | 1999 | 10428940 |
| molecular characterization of the genera proteus, morganella, and providencia by ribotyping. | the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ... | 1999 | 10449462 |
| isolation of providencia heimbachae from human feces. | providencia heimbachae was first described in 1986. it has been isolated from penguin feces and an aborted bovine fetus. to date, there has been no reported isolation of this organism from human specimens. we now report the isolation of p. heimbachae from the stool of a 23-year-old woman with idiopathic diarrhea. the identity of the human strain was determined biochemically and by dna relatedness to the type strain of p. heimbachae. | 1999 | 10449504 |
| development of bacterial resistance to the third generation cephalosporins and their clinical use. | development of gram-negative rods resistance to the third generation cephalosporins (cefotaxime, ceftazidime, cefoperazone) in connection with their application at the university hospital in olomouc was evaluated in this study. the highest increase in resistance to cefotaxime was detected in enterobacter cloacae (from 22.9% in 1995 to 49.0% in 1997) and enterobacter agglomerans strains (28.0% - 40.5%). in addition, increased resistance to ceftazidime in acinetobacter baumannii (12.5% - 35.1%), e ... | 1999 | 10465127 |
| identification of enterobacteriaceae bacteria by direct matrix-assisted laser desorptiom/ionization mass spectrometric analysis of whole cells. | several members of enterobacteriaceas were analyzed by matrix-assisted laser desorption ionization (maldi) time-of-flight mass spectrometry (tofms). characteristic mass spectral peaks and patterns were observed in the mass range of 2 to 20 kda. the mass peaks reported to be reproducibly observed by previous researchers, which were claimed to serve as species/strain-specific biomarkers, are consistently observed in our current study. despite the high degree of similarity found in the maldi mass s ... | 1999 | 10510415 |
| crystal structure of penicillin g acylase from the bro1 mutant strain of providencia rettgeri. | penicillin g acylase is an important enzyme in the commercial production of semisynthetic penicillins used to combat bacterial infections. mutant strains of providencia rettgeri were generated from wild-type cultures subjected to nutritional selective pressure. one such mutant, bro1, was able to use 6-bromohexanamide as its sole nitrogen source. penicillin acylase from the bro1 strain exhibited an altered substrate specificity consistent with the ability of the mutant to process 6-bromohexanamid ... | 1999 | 10548042 |
| two cases of fatal transfusion-associated bacterial sepsis provoked by providencia rettgeri. | 1999 | 10553171 | |
| providencia stuartii genes activated by cell-to-cell signaling and identification of a gene required for production or activity of an extracellular factor. | by utilizing reporter transposons, five providencia stuartii genes that are activated by the accumulation of self-produced extracellular signals have been identified. these genes have been designated cma for conditioned medium activated. the presence of conditioned medium from stationary-phase cultures grown in rich media resulted in the premature activation of each gene in cells at early log phase, with activation values ranging from 6- to 26-fold. preparation of conditioned medium from an m9 s ... | 1999 | 10572119 |
| comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens. | five chromogenic agar plates--cps id2 medium (biomérieux, france), chromagar orientation medium (becton dickinson, france), uriselect3 medium (sanofi diagnostics pasteur, france), rainbow agar uti medium (biolog, usa) and chromogenic uti medium (oxoid, germany)--for the detection, enumeration and direct identification of urinary tract pathogens were compared using 443 urine specimens at two hospital laboratories. the enumeration of microorganisms was consistent on the five media for 403 of the 4 ... | 1999 | 10614954 |
| [the results of a multicenter study of the comparative activity of cefepime and other antibiotics against the causative agents of severe hospital infections (the micromax program)]. | a multicentre trial was performed on the activity of cefepime in comparison with ceftazidime, ceftriaxone, piperacillin/tazobactam, imipenem and ciprofloxacin against severe hospital infection pathogens in intensive care units. the isolates of escherichia coli and proteus spp. from the majority of the centres were highly susceptible to the antibiotics (90 to 100 per cent of the isolates). in some centres up to 40 per cent of the isolates produced esbl. the isolates of klebsiella spp. were charac ... | 1999 | 10629736 |
| managing urinary tract infections in men. | despite the obvious genitourinary differences between the sexes, management of lower urinary tract infections in men is based largely on standards developed from studies in women. this has helped foster misconceptions that, among other problems, add needless complexity and expense to diagnosis and treatment of male patients. | 2000 | 10645989 |
| fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes. | intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ... | 2000 | 10648538 |
| bacterial resistance to disinfectants: present knowledge and future problems. | bacterial resistance to antibiotics is a long-established, widely-studied problem. increasingly, attention is being directed to the responses of various types of microbes to biocides (antiseptics, disinfectants and preservatives). different groups of bacteria vary in their susceptibility to biocides, with bacterial spores being the most resistant, followed by mycobacteria, then gram-negative organisms, with cocci generally being the most sensitive. there are wide divergencies within this general ... | 1999 | 10658759 |
| in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin. | the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ... | 2000 | 10747819 |
| in vitro susceptibility of gram-negative bacterial isolates to chlorhexidine gluconate. | to investigate the susceptibility of clinical isolates of gram-negative bacteria to chlorhexidine gluconate. | 1999 | 10750501 |
| assessment of the potential for microbial resistance to topical use of multiple antimicrobial agents. | the goal of this study was to reduce the likelihood of the generation and/or persistence of bacterial resistance to some antimicrobial components contained in a topical antimicrobial mixture (neomycin, polymyxin b, mupirocin and ciprofloxacin) for use with cultured skin grafts, by substitution of alternative antimicrobials, specifically fusidic acid for mupirocin and ofloxacin for ciprofloxacin. the alternative agents failed to serve that purpose. however, with the exception of specific genera o ... | 1999 | 10781215 |
| an approach for enhancing heterologous production of providencia rettgeri penicillin acylase in escherichia coli. | heterologous production of providencia rettgeri penicillin acylase (pac) was optimized in escherichia coli. several factors, including carbon, temperature, and host effects, were identified to be critical for the enzyme overproduction. the optimum culture conditions for the enzyme production vary for different host/vector systems. with the optimization, both volumetric and specific pac activities could be significantly improved by more than 50-fold compared to the native expression in p. rettger ... | 2000 | 10835229 |
| a kinetic study on the interaction between tazobactam (a penicillanic acid sulphone derivative) and active-site serine beta-lactamases. | the interaction between tazobactam and several chromosome- and plasmid-encoded (tem, shv, pse types) class a and c beta-lactamases was studied by spectrophotometry. tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner beta-lactam. a detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum beta-lactamases; the presence of a turn-over of acyl-enzyme ... | 2000 | 10850951 |
| in vitro activity of rifaximin, metronidazole and vancomycin against clostridium difficile and the rate of selection of spontaneously resistant mutants against representative anaerobic and aerobic bacteria, including ammonia-producing species. | rifaximin is a rifamycin derivative characterized by a wide antibacterial activity. this drug is neither absorbed by the gastrointestinal tract nor inactivated by gastric juices, and exerts its action entirely within the intestinal lumen. | 2000 | 10859431 |
| structure of an acidic o-specific polysaccharide of the bacterium providencia alcalifaciens o7. | an acidic o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium providencia alcalifaciens o7 and purified by gel chromatography followed by anion-exchange chromatography. on the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1h- and 13c-nmr spectroscopy, including two-dimensional 1h,1h homonuclear and h-detected 1h,13c heteronuclear correlation spectroscopy and nuclear o ... | 2000 | 10887286 |
| assessment of the microbiological quality and wash treatments of lettuce served in university restaurants. | one hundred and forty-four samples of lettuce from 16 university restaurants were analyzed. the mesophilic aerobic counts of all samples ranged from 3.01 to 7.81 log10 cfu g(-1). results of total coliforms ranged from < 0.47 to > 3.38 log10 most probable number (mpn) g(-1). of the lettuce samples, 25.7% harbored escherichia coli, 22.9% staphylococcus aureus and 84% group d streptococci. similarly, 10.4% of the samples harbored aeromonas hydrophila, 2.8% pseudomonas aeruginosa, and coliforms such ... | 2000 | 10898470 |
| sequence diversity of intervening sequences (ivss) in the 23s ribosomal rna in salmonella spp. | intervening sequences (ivss) occur sporadically in the rrl (ribosomal rna large) genes for 23s ribosomal rna (rrna) at helix-25 (base pair 550) and helix 45 (base pair 1170) in several bacterial genera, including salmonella, yersinia, proteus, and providencia, representing the enterobacteriaceae, but are missing from other genera such as escherichia. these sequences are transcribed, but later excised without re-ligation during rnaseiii processing of the rrna, resulting in fragmented 23s rrna. th ... | 2000 | 10925202 |
| hhv-8 is not a cofactor in the pathogenesis of environmentally induced malignant pleural mesothelioma. | after the recognition of human herpes virus 8 (hhv-8) in kaposi's sarcoma lesions, this new virus has been shown to be associated with various types of malignancy. one of them, body cavity-based lymphoma, is a high grade b-cell lymphoma arising from the body cavities. similarly, mesothelioma is a tumour that originates from the serosal linings of the pleural, pericardial and peritoneal cavities. one of the striking characteristics of mesothelioma cells is the secretion of interleukin-6 (il-6). a ... | 2000 | 10949869 |
| identification of escherichia coli ubib, a gene required for the first monooxygenase step in ubiquinone biosynthesis. | it was recently discovered that the aarf gene in providencia stuartii is required for coenzyme q (coq) biosynthesis. here we report that yigr, the escherichia coli homologue of aarf, is ubib, a gene required for the first monooxygenase step in coq biosynthesis. both the p. stuartii aarf and e. coli ubib (yigr) disruption mutant strains lack coq and accumulate octaprenylphenol. octaprenylphenol is the coq biosynthetic intermediate found to accumulate in the e. coli strain an59, which contains the ... | 2000 | 10960098 |
| classification, identification, and clinical significance of proteus, providencia, and morganella. | this review presents the current taxonomy of the genera proteus, providencia, and morganella, along with the current methods for the identification of each species within the three genera, incorporating both conventional biochemical and commercial methods. while all of these organisms are ubiquitous in the environment, individual case reports and nosocomial outbreak reports that demonstrate their ability to cause major infectious disease problems are presented. lastly, anticipated antimicrobial ... | 2000 | 11023955 |
| gut bacteria may be involved in interactions between plants, herbivores and their predators: microbial biosynthesis of n-acylglutamine surfactants as elicitors of plant volatiles. | n-acylamino acids are dominant and widespread constituents of insect oral secretions (regurgitants), serving the insect as biosurfactants in the digestive process. during feeding the conjugates may be introduced into damaged leaves and contribute there to the elicitation of plant defenses such as the induction of volatile biosynthesis. from gut segments of spodoptera exigua, mamestra brassicae and agrotis segetum 23 bacterial strains were isolated, ten of which were able to synthesise typical le ... | 2000 | 11030433 |
| classification of proteus vulgaris biogroup 3 with recognition of proteus hauseri sp. nov., nom. rev. and unnamed proteus genomospecies 4, 5 and 6. | strains traditionally identified as proteus vulgaris formed three biogroups. biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as proteus penneri. biogroup 2, characterized by positive reactions for indole, salicin and aesculin, was shown by dna hybridization (hydroxyapatite method) to be a genetic species separate from biogroup 1 and from biogroup 3 which is positive for indole production and negative for salicin an ... | 2000 | 11034498 |
| providencia may help find a function for a novel, widespread protein family. | 2000 | 11050401 | |
| natural antibiotic resistance of bacteria isolated from larvae of the oil fly, helaeomyia petrolei. | helaeomyia petrolei (oil fly) larvae inhabit the asphalt seeps of rancho la brea in los angeles, calif. the culturable microbial gut contents of larvae collected from the viscous oil were recently examined, and the majority (9 of 14) of the strains were identified as providencia spp. subsequently, 12 of the bacterial strains isolated were tested for their resistance or sensitivity to 23 commonly used antibiotics. all nine strains classified as providencia rettgeri exhibited dramatic resistance t ... | 2000 | 11055901 |
| gatifloxacin: a new fluoroquinolone. | gatifloxacin is a new 8-methoxy-fluoroquinoline antimicrobial agent. it has enhanced activity against gram-positive and atypical agents, while retaining broad-spectrum antigram-negative activity. for example, the mic(90) values for respiratory tract pathogens are < or = 0.5 microg/ml for organisms such as streptococcus pneumoniae (regardless of penicillin susceptibility), haemophilus influenzae (beta-lactamase positive or negative), moraxella catarrhalis (beta-lactamase positive or negative), le ... | 2000 | 11060784 |
| selective medium for primary isolation of members of the tribe proteeae. | a selective proteeae medium (spm) for isolation and preliminary detection of species of genera proteus, morganella, and providencia was evaluated. the spm contains tryptose phosphate agar with phenolphthalein monophosphate (as substrate for phosphatase activity), bile salts and polymyxin b (as inhibitors). the selectivity of the spm was tested by the ecometric method of quality assurance of culture media. fourteen reference cultures of enterobacteria and fifty-four strains of proteeae were teste ... | 1999 | 11097023 |
| beta-lactam susceptibility patterns and investigation of cephalosporin hydrolysing beta-lactamases of gram-negative extraintestinal clinical isolates. | of more than 3500 isolates of enterobacteriaceae, 48-69% were resistant to aminopenicillins and 11-45% to amoxycillin+clavulanic acid. resistance to second and third generation cephalosporins was present in 11-17 and 3-8% of escherichia coli, 47-56 and 15-52% of klebsiella-enterobacter, 36-57 and 16-27% of proteus, providencia and morganella isolates. pseudomonas aeruginosa strains varied in their resistance to antipseudomonal beta-lactams. isoelectric points, inhibitor profiles and substrate pr ... | 2000 | 11118847 |
| evaluation of current activities of fluoroquinolones against gram-negative bacilli using centralized in vitro testing and electronic surveillance. | given the propensity for enterobacteriaceae and clinically significant nonfermentative gram-negative bacilli to acquire antimicrobial resistance, consistent surveillance of the activities of agents commonly prescribed to treat infections arising from these organisms is imperative. this study determined the activities of two fluoroquinolones, levofloxacin and ciprofloxacin, and seven comparative agents against recent clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter b ... | 2001 | 11120976 |
| diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae. | the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ... | 2000 | 11126551 |
| formation of chromosomal tandem arrays of the sxt element and r391, two conjugative chromosomally integrating elements that share an attachment site. | the sxt element, a conjugative, self-transmissible, integrating element (a constin) originally derived from a vibrio cholerae o139 isolate from india, and incj element r391, originally derived from a south african providencia rettgeri isolate, were found to be genetically and functionally related. both of these constins integrate site specifically into the escherichia coli chromosome at an identical attachment site within the 5' end of prfc. they encode nearly identical integrases, which are req ... | 2001 | 11157923 |
| isolation and identification of bacteria associated with adult laboratory mexican fruit flies, anastrepha ludens (diptera: tephritidae). | from the guts of new and old colonies (female and male) of mexican fruit flies, anastrepha ludens (diptera: tephritidae), we identified a total of 18 different bacterial species belonging to the family enterobacteriaceae, pseudomonadaceae, vibrionaceae, micrococcaceae, deinococcacea, bacillaceae, and the genus listeria. enterobacter, providencia, serratia, and staphylococcus spp. were the most frequently isolated genera, with citrobacter, streptococcus, aerococcus, and listeria found less freque ... | 2001 | 11178731 |
| molecular analysis of clinical isolates of providencia alcalifaciens. | in an attempt to elucidate the virulence factors and the pathogenic mechanisms of providencia alcalifaciens, 36 isolates identified in 1994-1995 in recife city, brazil were analysed by pcr to investigate the presence of dna sequences homologous to virulence genes described in other invasive enterobacteria, as well as their ability to invade hela cells, their plasmid profiles and antibiotic resistance patterns. the genetic diversity of the isolates was also analysed by rapd-pcr. no homologous seq ... | 2001 | 11192501 |
| new tem variant (tem-92) produced by proteus mirabilis and providencia stuartii isolates. | the sequences of the bla(tem) genes encoding tem-92 in proteus mirabilis and providencia stuartii isolates were determined and were found to be identical. except for positions 218 (lys-6) and 512 (lys-104), the nucleotide sequence of bla(tem-92) was identical to that of bla(tem-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a g-162-->t substitution. the deduced amino acid sequence of tem-92 differed from that of tem-52 by the presence of a substituti ... | 2001 | 11257046 |
| role of sspa in the density-dependent expression of the transcriptional activator aarp in providencia stuartii. | the aarp protein in providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2')-ia gene. in addition, aarp activates genes involved in a multiple antibiotic resistance (mar) phenotype. expression of an aarp-lacz fusion increased in a density-dependent manner and reached peak levels at stationary phase. the expression of an aarp-lacz fusion could be prematurely activated in cells at early to mid-exponential phase by the a ... | 2001 | 11257543 |
| prevalence and growth of pathogens on salad vegetables, fruits and sprouts. | a total of 120 samples, comprising different types of raw vegetables (seven), fruits (three) and sprouts (three) obtained from street vendors, were tested for aerobic plate count, coliform count and various food-borne pathogens. average aerobic plate counts for salad vegetables, fruits and sprouts were greater than 10(10) cfu/g and 10(9) cfu/g respectively. pathogens isolated were s. aureus, e. coli, enterobacter sp., klebsiella sp., s. typhi, serratia sp., providencia sp. and p. aeruginosa. the ... | 2001 | 11279816 |
| a case of purple urine bag syndrome associated with providencia rettgeri. | 2001 | 11328846 | |
| antibiotic selective pressure and development of bacterial resistance. | this study evaluates the development of resistance in gram-negative rods to cefotaxime and ceftazidime, ofloxacin and ciprofloxacin, gentamicin and amikacin, meropenem and ampicillin/sulbactam over a five year period of use (1994-1998) at the university hospital in olomouc, czech republic. the development of bacterial resistance was linked with antibiotic use and hence selective pressure which was specific for the type of antibiotic and the bacterial species. statistically significant correlatio ... | 2001 | 11337221 |
| structure and serological characterization of an nepsilon-[(r)-1-carboxyethyl]-l-lysine-containing o-chain of the lipopolysaccharide of proteus mirabilis o13. | in this paper we present the structure and describe serological properties of the o-specific polysaccharide of proteus mirabilis o13 lipopolysaccharide, which contains a unique component: an amide of d-galacturonic acid (d-gala) with an unusual amino acid, nepsilon-[(r)-1-carboxyethyl]-l-lysine (alaninolysine, alalys). selective chemical degradations of either gala or alalys resulted in the loss of the serological reactivity of the polysaccharide with anti-o serum against p. mirabilis o13. neith ... | 2001 | 11348021 |
| mechanical carrier of bacterial enteric pathogens by chrysomya megacephala (diptera: calliphoridae) in chiang mai, thailand. | chrysomya megacephala was studied regarding its mechanically bacterial carrier in urban areas of chiang mai, northern thailand. fifty-six adult flies were randomly collected using sweep insect net during april-may, 1999 from 3 fresh food markets and examined for bacteriological isolation. among them, 49 flies (87.5%) were bacterial carriers. the total 22 bacterial species and 8 groups were isolated. three species previously reported as the bacterial enteric pathogens causing diarrheal disease we ... | 2000 | 11414447 |
| bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards. | a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ... | 2001 | 11417826 |
| activity of levofloxacin and ciprofloxacin against urinary pathogens. | this study compares the antibacterial activities of levofloxacin and ciprofloxacin against recently isolated urinary tract pathogens, by evaluating their mics and mbcs in accordance with nccls susceptibility tests, time-kill curves and interference with bacterial adhesion to uroepithelial cells. a total of 200 clinical isolates was tested, including the species escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, providencia rettgeri, pseudomonas aeruginosa, enterococcus ... | 2001 | 11418511 |
| [bacteremia due to providencia rettgeri]. | 2001 | 11440672 | |
| comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study. | the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ... | 2001 | 11448564 |
| overexpression and characterization of the chromosomal aminoglycoside 2'-n-acetyltransferase of providencia stuartii. | the gene coding for aminoglycoside 2'-n-acetyltransferase ia [aac(2')-ia] from providencia stuartii was amplified by pcr and cloned. the resulting construct, packf2, was transferred into escherichia coli for overexpression of aac(2')-ia as a fusion protein with an n-terminal hexa-his tag. the fusion protein was isolated and purified by affinity chromatography on ni(2+)-nitrilotriacetic acid agarose and gel permeation chromatography on superdex 75. comparison of the specific activity of this enzy ... | 2001 | 11451680 |
| chemical composition and cytotoxic and antimicrobial activity of calycotome villosa (poiret) link leaves. | the chemical composition of the essential oil and methanol extract of calycotome villosa (poiret) link leaves collected in sardinia (italy) has been studied by analytical and spectroscopic methods. falcarinol and some alcohols, terpenes, furan derivatives, and paraffins have been isolated from the essential oil. thirteen alkaloids and falcarinol have been identified in the chloroform fraction of the basic methanol extract. six flavonoids and four anthraquinones have been isolated in the chlorofo ... | 2001 | 11482772 |
| [reef fishes community structure in 4 atolls of the san andrés - providencia archipelago (southwestern caribbean)]. | in 1994 and 1995, 131 visual censuses of reef fishes were made using the stationary sampling method in courtown, albuquerque, serrana and roncador, four atolls of the archipelago of san andrés and old providence in the southwestern caribbean. fish species and their abundances were recorded in four geomorphologic zones: lagoon, windward barrier reef, windward terrace and forereef terrace. a total of 98 species were censused; the most abundant were chromis cyanea (14%), clepticus parra (14%) and s ... | 2000 | 11487934 |
| further studies on rpos in enterobacteria: identification of rpos in enterobacter cloacae and kluyvera cryocrescens. | rpos, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. to determine the presence of this gene in enteric bacteria, an escherichia coli rpos probe was constructed and used to detect the presence of this gene in different species. a gene homologous to rpos was found in citrobacter amalonaticu ... | 2001 | 11491080 |
| urinary tract infections in patients with spinal cord lesions: treatment and prevention. | even though the mortality due to urinary tract complications has decreased dramatically during the last decades in individuals with spinal cord lesions (scl), urinary tract infections (uti) still cause significant morbidity in this population. complicated uti are caused by a much wider variety of organisms in individuals with scl than in the general population and are often polymicrobial. escherichia coli, pseudomonas spp., klebsiella spp., proteus spp., serratia spp., providencia spp., enteroco ... | 2001 | 11511022 |
| comparative antimicrobial spectrum and activity of bms284756 (t-3811, a desfluoroquinolone) tested against 656 enterobacteriaceae, including preliminary in vitro susceptibility test comparisons and development. | bms284756 (t-3811), a novel des-f(6)-quinolone, was evaluated using isolates of enterobacteriaceae from the sentry antimicrobial surveillance program tested by etest (ab biodisk, solna, sweden), reference broth microdilution and disk diffusion (5-microg) methods. ciprofloxacin, levofloxacin, gemifloxacin and gatifloxacin were also tested by broth microdilution as comparator antimicrobial agents within the same drug class. the 656 isolate collection included species from the genera citrobacter, e ... | 2001 | 11516937 |
| intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae. | intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ... | 2001 | 11544246 |
| a large outbreak of foodborne infection attributed to providencia alcalifaciens. | the enteropathogenicity of providencia alcalifaciens, a member of the family enterobacteriaceae, has not yet been well established. in november 1996, a large outbreak of foodborne infection occurred in fukui, japan. in this study, the etiology of the outbreak was investigated. no other recognized enteropathogens were detected in patient fecal samples, but p. alcalifaciens was detected in 7 of 18 samples. the isolates were found to be clonal by pulsed-field gel electrophoresis. the patients who p ... | 2001 | 11574921 |
| antibacterial activities and pharmacokinetics of e-4767 and e-5065, two new 8-chlorofluoroquinolones with a 7-azetidin ring substituent. | e-4767 [(-)-7-[3-(r)-amino-2-(s)-methyl-1-azetidinyl]-8-chloro-1-cyclopropyl-1,4-dihydro-6-fluoro-4-oxo-3-quinolinecarboxylic acid] and e-5065 [(-)-7-(3-amino-1-azetidinyl)-8-chloro-1-cyclopropyl-1,4-dihydro-6-fluoro-4-oxo-3-quinolinecarboxylic acid] are two new chlorofluoroquinolones with an azetidine moiety at position 7. their in vitro activities were evaluated in comparison with those of ciprofloxacin, ofloxacin, fleroxacin, and tosufloxacin, while ciprofloxacin was used as a reference for i ... | 2001 | 11600365 |
| non-invasive providencia alcalifaciens strains fail to attach to hep-2 cells. | we investigated the adherence properties of six p. alcalifaciens strains with previously characterized differential invasive capabilities in hep-2 cells. highly invasive strains were found to attach to hep-2 cell monolayers within 2 h post-infection and in large numbers on the eukaryotic cell surfaces within 3 h post-infection. in contrast, weakly or non-invasive p. alcalifaciens strains were non-adherent to hep-2 cells even at 3 h post-infection. highly invasive isolates were found to weakly bi ... | 2001 | 11685508 |
| susceptibility to fluoroquinolones among commonly isolated gram-negative bacilli in 2000: trust and tsn data for the united states. tracking resistance in the united states today. the surveillance network. | from january to may 2000, as part of the tracking resistance in the united states today (trust) surveillance initiative, clinical isolates of enterobacteriaceae (n=2519) and non-fermentative gram-negatives (n=580) were prospectively collected from 26 hospital laboratories across the united states. isolates were tested for susceptibility to three fluoroquinolones (ciprofloxacin, levofloxacin, gatifloxacin) and seven other agents. in addition, data for the same period were collected from the surve ... | 2002 | 11814764 |
| marine bacteria cause false-positive results in the colilert-18 rapid identification test for escherichia coli in florida waters. | the colilert-18 system for enumeration of total coliforms and escherichia coli is approved by the u.s. environmental protection agency for use in drinking water analysis and is also used by various agencies and research studies for enumeration of indicator organisms in fresh and saline waters. during monitoring of pinellas county, fla., marine waters, estimates of e. coli numbers (by colilert-18) frequently exceeded fecal coliform counts (by membrane filtration) by 1 to 3 orders of magnitude. sa ... | 2002 | 11823188 |
| primary bacterial enteritis caused by providencia alcalifaciens in three dogs. | 2002 | 11833525 | |
| cq-397 and cq-414: antimicrobial activity and spectrum of two fluoroquinolone---cephalosporin, dual-action compounds with carboxamido bonds. | objective: to evaluate the potential spectrum of activity of two novel dual-action compounds with carboxamido bonds (cq-397 and cq-414; laboratorios aranda, san rafael, mexico) against human pathogens. methods: approximately 800 gram-positive and gram-negative aerobic clinical bacteria were tested in vitro using the mueller-hinton broth microdilution method of the national committee of clinical laboratory standards. results: cq-397 (cefamandole+enrofloxacin) and cq-414 (cefamandole+norfloxacin) ... | 1997 | 11864130 |
| evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies. | phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ... | 2002 | 11931166 |
| prevalence of extended spectrum beta-lactamases among enterobacteriaceae: an italian survey. | the prevalence of extended-spectrum beta-lactamase (esbl) production by consecutive non-repeated isolates of enterobacteriaceae was determined over a 6-month period. a total of 8015 strains were isolated from ten italian laboratories and 509 (6.3%) of these were designated esbl producers from the results of a double-disk synergy test. escherichia coli was the most isolated microrganism, followed by klebsiella pneumoniae and proteus mirabilis. providencia stuartii (28.1%) was the most frequently ... | 2002 | 11932144 |
| high-level secretory expression of penicillin amidase from providencia rettgeri in saccharomyces cerevisiae: purification and characterization. | heterologous production of the heterodimeric penicillin g amidase (pac) from providencia rettgeri was optimized in saccharomyces cerevisiae. several factors, including the effect of different growth and induction conditions, were identified to be critical for the enzyme overproduction and secretion. the pac yield was significantly increased by more than 500-fold compared to that obtained in the native bacterium, and the recombinant enzyme was almost entirely secreted. electrophoretic characteriz ... | 2002 | 11934304 |
| providencia alcalifaciens in diarrhoeic dogs and cats. | 2002 | 11936417 | |
| hyperimmunoglobulin-e syndrome. | two saudi girls aged 8 years and 5 years were seen over a period of 6 years and 5 years. their clinical presentations consisted of recurrent bilateral otitis media, repeated episodes of tonsillitis and chest infection. cultures from the ears grew on numerous occasions staphylococcus aureus, streptococcus pneumoniae, pseudomonas aeroginosa, proteus species and providencia species. the 8-year-old had a serum ige level of 1431 iu/l, with normal levels of other immunoglobulin classes. the 5-year-old ... | 2002 | 11953776 |
| [nosocomial urinary stent infection at the urology service of the cocody university hospital center]. | one of the most common complication of urinary stent is urinary infection. at the cocody university, 28 men and 2 females who had a urinary stent were evaluated for urinary infection, bacterial growth, and their sensitivity to antibiotics. in 85.71% of the cases, the bacteriologic analysis was positive for a mean duration of urinary drainage of 32, 62 days (range 03 to 90 days). bacterial isolates were: 11 cases of klebsiella spp, 5 cases of providencia spp; 1 case of escherichia coli, 1 case of ... | 1999 | 11957281 |
| [post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria]. | as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ... | 2002 | 11977920 |
| susceptibility of antibiotic-resistant gram-negative bacteria to biocides: a perspective from the study of catheter biofilms. | bacteria resistant to both the agents deployed to prevent infections and those used to treat infections would be formidable nosocomial pathogens. the aim of this paper is to review the evidence that gram-negative bacteria resistant to antibiotics and biocides have emerged and been responsible for catheter-associated urinary tract infection. a study of patients undergoing intermittent bladder catheterization revealed that the frequent application of the antiseptic chlorhexidine to the perineal sk ... | 2002 | 12000625 |
| effect of ph on high-temperature production of bacterial penicillin acylase in escherichia coli. | high-temperature-oriented production of bacterial penicillin acylase (pac), which is usually expressed at low temperatures (less than 30 degrees c), was demonstrated in this study via heterologous expression of the providencia rettgeri (p. rettgeri) pac gene in escherichia coli (e. coli). while it is possible to produce pac at a temperature as high as 37 degrees c, the environmental condition (specifically, culture ph) critically affected culture performance. production of pac at 37 degrees c wa ... | 2002 | 12052092 |