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synthesis and excretion of polygalacturonic acid trans-eliminase in erwinia, yersinia, and klebsiella species. 1979371775
[cefazedone in infectious diseases in gynecology. clinical tests on efficacy and tolerance].a clinical trial on efficacy and tolerance of cefazedone, a new semisynthetic cephalosporine-derivate, was carried out in a gynaecological clinic on 75 patients. in 38 of these cases, besides the gynaecological affection, urinary tract infections were determined microbiologically (e. coli, p. mirabilis, klebsiella or enterococci). inflammation of the lower abdominal area was established in the remaining 37 patients (adnexitis, pelvic peritonitis, endometritis, salpingitis). the average duration ...1979372073
klebocin typing of klebsiella pneumoniae. 1979372098
trimethoprim in combination with a sulfonamide for oral treatment of canine urinary tract infections.trimethoprim, in combination with sulfadiazine or sulfamethoxazole was administered orally for 7 to 14 days to 84 dogs with urinary tract infections (uti). the daily dosage of 26.4 mg/kg (12 mg/lb) was divided into 2 equal parts and administered at about 12-hour intervals. response to treatment, based on negative urine culture during or after therapy, was 37 of 45 (82%) for uti caused by escherichia coli, 11 of 15 (73%) uti caused by proteus mirabilis, 8 of 12 (67%) uti caused by klebsiella pneu ...1979372165
cefuroxime therapy for urinary tract infections caused by a multi-resistant, epidemic klebsiella aerogenes. 1979372170
sensitivity, precision, and accuracy of the y1 adrenal cell enterotoxin assay.the y1 adrenal cell assay for heat-labile enterotoxin (lt) was found to be relatively insensitive for filtrates of toxigenic escherichia coli h10407. when observations were made blindly and subjected to rigorous controls, reliable detection of lt occured only at filtrate dilutions from 1:4 to 1:10. detection of lt was unreliable when e. coli h10407 was mixed with another enteric organism. overall, 7.1% of observers' reports from a single assay were imprecise, and 2.6% would have resulted in erro ...1979372224
reproducibility of an indirect immunofluorescent-antibody technique for capsular serotyping of klebsiella pneumoniae.reproducibility of capsular serotypes of 55 consecutive clinical isolates of klebsiella pneumoniae was evaluated by an indirect immunofluorescent-antibody technique previously described by riser et al. (j. clin. pathol. 29: 296-304, 1976) five colonies per specimen were examined for colony-to-colony variation, day-to-day variation, and reader-to-reader variation. seventy-two reference strains were tested with each of 18 pools and 72 specific antisera prior to the clinical specimens to determine ...1979372225
the development and assessment of a bacteriocin typing method for klebsiella.klebsiellas are generally typed by the method of capsular serotyping but, although this is a reliable method, it is time consuming, requires the production of a large number of antisera and is not generally available. for this reason another method for typing klebsiellas was sought. a bacteriocin typing method involving mitomycin c induction was developed and the cultural conditions giving optimum klebecin production and the best methods of testing the sensitivity of the organisms to klebecins w ...1979372440
the variable response of bacteria to excess ferric iron in host tissues.the enhancement by exogenous ferric iron, both systemic and local, of the infectivity of 120 strains of bacteria, representing 17 genera, was measured in the skin of guinea-pigs. systemic iron enhanced only 23% of 115 strains, and local iron 49% of 71 strains. systemic iron, by an apparently anti-inflammatory action, depressed the size of lesions produced by 27 of the non-enhanced strains from nine of the genera tested. for most strains, the degree of enhancement was small, ranging from 2- to 8- ...1979372534
antibody-coated bacteria in persistent and recurrent urinary tract infections. 1978372670
integration of a transposable dna sequence which mediates ampicillin resistance into clo df13 plasmid dna: determination of the site and orientation of tna insertions. 1978372963
[a case of skeletal dissemination of septic foci with disastrous outcome (proceedings)]. 1978373023
[experimental studies to culture bacteria from blood specimen with an addition of ampicillin in various nutrient media (author's transl)].ampicillin-sensitive or -resistant staphylococcus aureus and klebsiella strains were cultured in various nutrient media as prototypes of the agents of sepsis isolated in bacteriological routine analysis. in each case, 2 ml of defibrinated human blood containing 100 and 1000 cells, 8 gamma and 80 gamma ampicillin/1 ml blood respectively were added to 50 ml of nutrient medium. the following media were used. 1. casein soya-bean meal peptone broth (oxoid), 2. tryptose-phosphate medium (oxoid), 3. de ...1978373322
experience with a quantitative carrier test for the evaluation of disinfectants. 1978373325
[investigations on the antimicrobial activity of amine-aldehyde-condensates. 6. communication: azomethines of substituted benzaldehydes (author's transl)].in the scope of our research about the antimicrobial activity of amin-aldehydecondensates a number of substituted benzaldehyde azomethines was synthesized by condensation of suitable benzaldehydes with primary amines (aminoethanol and n-butylamine). structures and physical constants are shown in the table 1. the antimicrobial activity is demonstrated by the results of the disk-test (table 2 and 3), of the minimal inhibition concentration test (mic, table 4) and of the suspension-disinfecting tes ...1978373326
lymph follicular cholecystitis.ten cases of cholecystitis with severe lymphocytic reactions were selected among serially examined 131 cases of cholecystitis or/and cholelithiasis. gram-negative bacterial infection, especially e. coli and k. pneumonia seemed to be related as the cause of these severe lymphocytic reactions, but the gallstone revealed no definite influence. we would like to separate the cases with severe lymphocytic reactions as a lymph follicular cholecystitis and consider the possibility of a gram-negative bac ...1979373378
limitations of the moeller lysine and ornithine decarboxylase tests.a total of 40 fecal and environmental isolates, including 26 escherichia coli strains, 9 members of the genus klebsiella, and 5 members of the genus enterobacter, were tested by enzyme assay for their endogenous and induced levels of lysine decarboxylase and ornithine decarboxylase when grown in moeller decarboxylase medium. all of the coliforms examined had measurable lysine decarboxylase and ornithine decarboxylase activities whether or not they were positive in the moeller test. in general, t ...1979373624
histamine production by klebsiella pneumoniae and an incident of scombroid fish poisoning.a histamine-producing strain of klebsiella pneumoniae was isolated from a sample of tuna sashimi implicated in an outbreak of scombroid fish poisoning. none of the other nine gram-negative bacterial strains isolated from the tuna sashimi was capable of equivalent histamine production. bacterial histamine production was monitored in a tuna fish infusion broth (tfib), and the implicated k. pneumoniae was capable of producing 442 mg of histamine per 100 g of tuna in tfib in 7 h under controlled inc ...1979373626
klebsiella pneumoniae. 1978373650
endotracheally administered antibiotics for gram-negative bronchopneumonia.sisomicin or a placebo was administered endotracheally to two groups of 18 and 20 unconscious patients, respectively, who had tracheostomies or endotracheal tubes in place and developed a severe gram-negative broncho-pneumonia. in addition, the patients received systemically a combination of sisomicin and carbenicillin. a favorable clinical response was obtained in 14 (77 percent) of the 18 patients who were treated with sisomicin and in nine (45 percent) of the 20 patients who received the plac ...1979374017
overflow metabolism in aerobic micro-organisms. 1979374156
association of tellurium resistance and bacteriophage inhibition conferred by r plasmids.concomitant resistance to tellurium compounds (ter) and inhibition of coli-phage development (phi) are properties mediated by many h2 incompatibility group r plasmids which have been isolated from diverse bacterial and geographic sources. ter plasmids from tellurium-resistant bacteria that were isolated from sewage and industrial wastes also mediated phage inhibition. of these ter plasmids, three from citrobacter freundii belonged to the h incompatibility group, whereas three from klebsiella pne ...1979374351
antimicrobial therapy of septicemia due to klebsiella pneumoniae in neutropenic rats.three different isolates of klebsiella pneumoniae, highly sensitive to amikacin but varying in susceptibility to cefazolin, were injected intraperitoneally into neutropenic rats. animals were treated every 8 hr for 72 hr with saline (controls), cefzolin (full dose, 40 mg/kg; one-fourth dose, 10 mg/kg), amikacin (full dose, 8 mg/kg; one-fourth dose, 2 mg/kg), or a combination of both drugs at either full dose or one-fourth dose. all drugs were given intramuscularly. combination therapy with full ...1979374646
spontaneous degradation of prd1 dna into unique size classes is reca dependent.the his and nif genes of the p1 type plasmid prd1 were lost at a high frequency in a reca+ but not in a reca- escherichia coli host during growth in a non-selective medium. 92% of the his- nif- segregants after 6 subcultures retained the genetic markers of the precursor plasmid rp4, while the remainder lost all of the prd1 markers with the concomitant loss of ccc-dna. plasmids purified from the his- nif- segregants resembled rp4 in the physical and genetic properties examined. the contour length ...1979375020
the mutagenic action of nitroimidazoles. iv. a comparison of the mutagenic action of several nitroimidazoles and some imidazoles.the mutagenic action of 51 imidazoles was investigated. the fluctuation test of luria and delbrück was used, with klebsiella pneumoniae as test organism. 8 compounds, including 5 with a weak mutagenic action in the fluctuation test, were also investigated by the ames test in which salmonella typhimurium ta100 was used. of the 51 imidazoles examined, 33 were nitroimidazoles. 31 of the latter appeared to be mutagenic, whereas out of the 18 other imidazoles without a nitro group only 2 were mutagen ...1979375080
bacteriophage specificity in the identification of yersinia pestis as compared with other enterobacteria.bacteriophage typing of yersinia pestis and the specificity of the phage among enterobacteriaceae were investigated. the bacteriophage used for rapid identification of y. pestis reacted with representative strains of all recognized species of shigella as well as with salmonella cholerae-suis. reactive shigella serotypes were sh. dysenteriae 1 and 9, sh. flexneri 2a, sh. boydii 1 and 6, and sh. sonnei. patterns consisting of isolated plaques (two cases) or absence of plaques were observed when th ...1978375327
[probability of resistance to 10 standard- and 7 recently introduced antimicrobics in multiresistant strains of escherichia coli, proteus mirabilis and klebsiella spec (author's transl)]. 1979375624
[multiple drug resistance in different medical institutions: frequency and resistance patterns in escherichia coli, proteus mirabilis and klebsiella spec (author's transl)]. 1979375625
gram-negative bacillary sepsis associated with use of lipid emulsion in parenteral nutrition. 1979375720
enteric gram-negative rods, 1976. 1979375729
[structure of the microflora of suppurative wounds and its sensitivity to antibiotics].clinico-bacteriological examination of patients with purulent infections showed that staphylococcus was the predominating microflora in the wounds. simultaneously an increasing role of gram-negative conditionally pathogenic bacteria was shown. multiple drug resistance was found in the organisms tested. the highest sensitivity levels were observed to gentamicin, kanamycin, tetracycline, levomycetin. it was shown by means of special typing methods that staphylococci of phage group iii and ps. aeru ...1979375824
coenzyme b12-dependent diol dehydrase: purification, subunit heterogeneity, and reversible association. 1979375836
nosocomial klebsiella pneumoniae epidemic in a neonatal special care unit. 1978376441
an investigation into the incidence and sources of klebsiella infections in hospital patients.coliforms isolated from infections over a period of 22 months were examined and 16% were shown to be klebsiellas. the biochemical reactions and serotypes of these klebsiellas were determined and the relationship between species, serotype and type of infection investigated. although no obvious outbreaks of infection occurred during this period a number of clusters of isolations of the same serotype of klebsiella were found. using an enrichment method for the isolation of klebsiellas from faeces, ...1979376698
septicemia in association with acute lymphoblastic leukemia.fifty consecutive episodes of septicemia were studied in 41 children who had acute lymphoblastic leukemia. seventy-six percent of these episodes occurred when the absolute granulocyte count was 200/mm3 or less and were caused by gram-negative enteric and gram-positive mucocutaneous bacteria. in eight patients, streptococcus pyogenes was isolated at the time when all was diagnosed. multiple anaerobic and aerobic isolates from a single blood culture were associated with abdominal distress, whereas ...1979376800
detection of neonatal bacteremia. 1979376806
nursing care study. patient on a ventilator: beyond the speech barrier. 1979377248
plasmids and transposons and their stability and mutability in bacteria isolated during an outbreak of hospital infection. 1979377321
a comparison of the antibacterial activity of sulphonamide-trimethoprim combinations.sensitivity patterns of 326 clinical isolates toward sulphonamide/trimethoprim combinations were tested in vitro. the relative merits of the different combinations are discussed in their pharmacological and bacteriological contexts. it is concluded that organisms sensitive to cotrimoxazole as judged by in vitro sensitivity tests performed at 37 degrees c are also sensitive to sulphamoxole/trimethoprim.1979377523
[antibacterial effect of ankerplast spray].the diffusion test yielded no satisfactory results. in the tube test, both the spray and the solvent (in a dilution of 1:4 and 1:8, respectively) proved to be bacteriostatically active against enterococci, staphylococcus aureus, escheria coli, proteus, and pseudomonas aeruginosa. these germs were killed within 30 minutes in the test for bactericidal activity, whereas the reference substance (1% phenol) required 1--2 hours, except against the last-named germ. even the copolymer exerted a bacteric ...1979377580
the impact of hospitalization on the aerobic fecal microflora.quantitative bacteriological analysis of the aerobic fecal microflora of 75 patients indicated that, at the time of admission to hospital, escherichia coli were the predominant fecal aerotolerant bacteria. subsequent fecal samples showed a progressive supplantation of e coli by klebsiella, enterobacter and proteus. at the end of 21 days of hospitalization, e coli remained predominant in only 30 patients. none of the patients had received antibiotics, undergone surgery or been subjected to x-ray ...1979377964
characterization of motile and acetoin-negative klebsiella pneumoniae strains by dna: dna by hybridization.hybridization studies were carried out to determine the extent of divergence between different members of the enterobacteriaceae and group d, previously described by numerical analysis of phenotypic characters, and comprising strains isolated from surface waters. this group, apparently belonging in or related to the genus citrobacter on the basis of the imvic tests differs from this taxon by numerical analysis and dna base composition. our work reveals a dna relatedness of 81-89% between the cen ...1978378119
respiratory nitrate reductase: its localization in the cytoplasmic membrane of klebsiella aerogenes and bacillus licheniformis.the sidedness of the respiratory nitrate reductase in the cytoplasmic membrane of bacillus licheniformis and klebsiella aerogenes was studied by indirect immunofluorescence and by lactoperoxidase-catalyzed iodination. it was shown that the two subunits (mr 150000 and 57000, respectively) of nitrate reductase of b. licheniformis are localized on the cytoplasmic side of the membrane, whereas the k. aerogenes enzyme is a transmembrane protein. the different localization of nitrate reductase in the ...1979378657
the possible role of catabolic plasmids in bacterial steroid degradation. 1979378710
capsular serotypic specificity of the protection conferred on mice by klebsiella pneumoniae ribosomal preparations.klebsiella pneumoniae ribosomal preparations protect mice immunized by the subcutaneous route against an intraperitoneal challenge of 100 50% lethal doses. the minimal protective doses are 5 and 0.4 micrograms of proteins for preparations extracted from strains of capsular serotypes 1 and 2, respectively. this difference in protective activity is also found in bacteria killed by formalin. the protective activity of these preparations is not diminished by their purification on sucrose gradient, w ...1979378855
order of genes near nif in klebsiella pneumoniae.analysis of strains with deletions of all or part of nif have ordered the klebsiella pneumoniae genetic loci as thi rbt dal udk gnd rfb has nif shia. the his-nif plasmids prd1 and ptm4010 contain the genes gnd rfb his nif shia.1979378930
fermentation of 1,2-propanediol with 1,2-ethanediol by some genera of enterobacteriaceae, involving coenzyme b12-dependent diol dehydratase.klebsiella pneumoniae (aerobacter aerogenes) atcc 8724 was able to grow anaerobically on 1,2-propanediol and 1,2-ethanediol as carbon and energy sources. whole cells of the bacterium grown anaerobically on 1,2-propanediol or on glycerol catalyzed conversion of 1,2-diols and aldehydes to the corresponding acids and alcohols. glucose-grown cells also converted aldehydes, but not 1,2-diols, to acids and alcohols. the presence of activities of coenzyme b(12)-dependent diol dehydratase, alcohol dehyd ...1979378959
introduction of bacteriophage lambda into cells of klebsiella aerogenes.we have shown that a mutation in the cro gene of phage lambda greatly reduces zygotic induction. this observation has allowed us to move this phage on an episome into cells of klebsiella aerogenes where it grows as well as in cells of escherichia coli. this technique should allow the introduction of various derivatives of lambda into any organism which is able to receive deoxyribonucleic acid from e. coli.1979378984
coliform mastitis--a review. 1979379056
mastitis: i. in vitro antimicrobial activity of alkyl amines against mastitic bacteria.the activities of branched and straight chain amines (10 to 18 carbons chain length) were compared in inhibiting the growth of five microorganisms that cause about 95% of bovine mastitis. three gram-positive (streptococcus agalactiae, streptococcus uberis, staphylococcus aureus) and two gram-negative (escherichia coli, klebsiella pneumoniae) bacteria were used in a trypticase soy broth tube culture growth assay. sixty-two compounds were screened at concentrations of 200, 100, 50, 25, 10, 5, and ...1979379061
enterotoxigenic enteric bacteria in foods and outbreaks of food-borne diseases in sweden.all of 86 food routinely examined for potentially pathogenic enteric bacteria were found to harbour one or more coliform species. none of the strains isolated produced heat-labile enterotoxin (lt) or showed invasive properties. the suckling mouse test indicated that one strain of escherichia coli produced heat-stable enterotoxin (st). twelve incidents of suspected food poisoning were also investigated. in two of them the foods examined contained lt-producing strains of e. coli and in two there w ...1979379211
acquisition of klebsiella aerogenes by neonates.the acquisition of klebsiella aerogenes by neonates born in hospital has been studied by sero- and klebecin typing. this organism was more commonly carried in the bowel of breast-fed babies than of bottle-fed babies. only very rarely did babies acquire strains of k. aerogenes from their mothers. k. aerogenes was widely distributed in the ward environment and on the hands of nurses and mothers. some strains were able to spread on the ward. these results are relevant to the control of k. aerogenes ...1979379340
[morphological studies on antibacterial activities of cefotiam (author's transl)].1. cefotiam was demonstrated to be more potent than cefazolin in its antibacterial activities against clinical isolates of e. coli, klebsiella, serratia, proteus mirabilis, proteus morganii and proteus inconstans. mics of cefotiam with 10(6) cells/ml inoculum size were considerably lower than those with 10(8) cells/ml. 2. organisms lysed when exposed to cefotiam at concentrations higher than the mics with 10(8) cells/ml. morphological changes of organisms into filament occurred even at concentra ...1979379387
the infected pacemaker pocket.between january, 1963, and september, 1978, a total of 1,789 pacemakers were implanted at henry ford hospital. infection at the site of implantation developed in 19 instances for an incidence of 1.06 percent. the most common organism cultured was staphylococcus epidermidis, and conservative treatment was successful with these patients. in all patients with organisms other than staphylococcus epidermidis, reimplantation of a new unit in a new, clean site was required.1979379440
biosynthesis of pullulanase, an outer membrane enzyme in klebsiella. differential effect of translation and transcription inhibitors on synthesis of enzymes in cytoplasm, periplasm, cytoplasmic and outer membrane. 1979379591
[a case of extremely severe chest injury with fracture of several ribs (author's transl)].a 72-years old man was severely injured when a lorry rolled back and pinned him down, causing contusion of the chest, fractures of ribs 3-10 on the right and haemothorax. treatment of the chest injuries was by drainage and by positive end-expiratory pressure ventilation because of the development of severe pneumonia with wet lung. persistent renal insufficiency, a gastro-intestinal haemorrhage and diabetes also required treatment. the patient developed septic endomyocarditis as a late complicati ...1979379848
circular dichroism and magnetic circular dichroism of nitrogenase proteins.circular dichroism (cd) and magnetic circular dichroism (mcd) spectra of nitrogenase components (mofe protein and fe protein) from azotobacter vinelandii (av) and klebsiella pneumoniae (kp) have been obtained in the near infrared-visible-near ultraviolet spectral region. previously, visible cd was reported to be absent or barely detectable in nitrogenase proteins; mcd spectra have not been reported. the chiroptical spectra can be measured in solution at room temperature, an advantage relative to ...1979379860
physical map of chromosomal nitrogen fixation (nif) genes of klebsiella pneumoniae.we describe a method for the rapid determination of the physical location of mutations caused by insertion of transposable elements. we used this method to construct a detailed physical map of the nitrogen fixation (nif) gene cluster of klebsiella pneumoniae and to correlate it with the genetic map. total cellular dna was isolated from individual strains, each carrying an insertion in 1 of 15 different nif genes. the dna was digested with a restriction endonuclease, fractionated by agarose gel e ...1979379866
antibiotic-resistant serratia marcescens infection in a hospital.over a 12-month period, 74 isolates of serratia marcescens were obtained from various sources at tygerberg hospital. the majority of these isolates were from catheterized patients with urinary tract infections, and were non-pigmented and resistant to all antibiotics tested, excepting amikacin and neomycin. all isolates transferred resistance to tobramycin, gentamicin and tetracycline by conjugation to escherichia coli recipients as separate markers at low frequency. a non-self-transmissible plas ...1979380020
problems in the diagnosis of urinary tract infections. 1979380219
acute suppurative thyroiditis. 1979380321
clindamycin in treatment of aspiration pneumonia in children.twenty-eight patients with anaerobic pleuropulmonary infections were treated with clindamycin alone or clindamycin with gentamicin. sixteen of the patients presented with pneumonitis, nine with necrotizing pneumonia, and three with lung abscesses. the average length of treatment was 13.8 days, and the duration of temperature after initiation of therapy was 3.1 days. the predominant isolates were anaerobic gram-positive cocci (23 isolates), bacteroides melaninogenicus (14), bacteroides fragilis ( ...1979380459
nosocomial multiply resistant klebsiella pneumoniae: epidemiology of an outbreak of apparent index case origin.a nosocomial epidemic of multiply resistant (mr) klebsiella pneumoniae characterized by resistance to gentamicin, tobramycin, kanamycin, cephalothin, chloramphenicol, and ampicillin occurred in a veterans administration hospital from 1975 to 1977. a total of 66 infected or colonized patients were observed in a 2-year period; there were 43 urinary tract infections, 13 wound or soft tissue infections, 8 pneumonias, and 6 patients with only asymptomatic stool colonization. four patients had both pn ...1979380463
physical characterization of ten r plasmids obtained from an outbreak of nosocomial klebsiella pneumoniae infections.gentamicin resistance in klebsiella pneumoniae involved in an outbreak at the minneapolis veterans administration hospital was due to a transmissible r plasmid. in addition to gentamicin, this plasmid conferred resistance to tobramycin, kanamycin, ampicillin, carbenicillin, cephalothin, chloramphenicol, and sulfathiazole. r plasmids which transferred this complex antibiogram were identified in several clinical isolates, including four different serotypes of k. pneumoniae, escherichia coli, enter ...1979380464
stereospecificity and mechanism of adenosylcobalamin-dependent diol dehydratase. catalysis and inactivation with meso- and dl-2,3-butanediols as substrates. 1979380560
bacteria causing symptomatic urinary tract infection or asymptomatic bacteriuria.one thousand bacterial isolates from consecutive suprapublic aspirations of urine in two groups of patients with symptomatic urinary tract infection and two groups with asymptomatic bacteriuria were studied. staphylococci accounted for 13 and 27% of the isolates from symptomatic patients but for only 3 and 4% of strains from asymptomatic bacteriuria. conversely, 18 and 30% of escherichia coli isolates from asymptomatic bacteriuria were auto-agglutinable when tested with antisera to the common ur ...1979381327
[sensitivity of frequent-occurring isolates in outpatients to routinely-used antibiotics (author's transl)].the susceptibility of microorganisms (staphylococcus aureus, streptococcus pyogenes, streptococcus pneumoniae, e. coli, klebsiella pneumoniae, salmonella spp. and haemophilus influenzae) frequently occurring in outpatients (treated by practitioners) to six kinds of antibiotics [ampicillin (abpc), cefazolin (cez), erythromycin (em), minocycline (mnc), gentamicin (gm) and thiamphenicol (tp)] was determined by the standard method established by the japan society of chemotherapy. 1) there were few m ...1979381708
the peculiar behaviour of coliphage p1vir mutants on restricting hosts.the sensitivity of two vir coliphage p1 mutants, to the host-specificity systems a, k, rii, and b of escherichia coli, and to the newly identified host-specificity systems mir and 59 of klebsiella pneumoniae has been studied. both 1 vir phages were completely resistant to the e. coli k, but were restricted 10(-5) by the b, mir and 59 systems. the p1 parent, on the contrary, was restricted 10(-2) by all of the above mentioned hosts. the possible role of phage coded proteins in the altered sensiti ...1978381845
different effects of the capsular polysaccharide of klebsiella pneumoniae on the functions of various subpopulations of b cells in the immune response of mice to t-dependent antigen. 1979381848
gentamicin resistance in christchurch hospitals.prior to june 1976, the isolation of gentamicin resistant organisms was an infrequent occurrence in north canterbury hospital board institutions. during july 1976, 20 different gentamicin resistant organisms were isolated from patients in christchurch hospital. gentamicin resistant organisms hav e been continually isolated from an increasingly wide area since then. the organisms involved are: providence species; pseudomonas aeruginosa; klebsiella species; e coli; staphyloccus aureus; proteus mir ...1979381994
aspects of genital infection and swabbing techniques in the mare.the three groups of bacteria that cause endometritis in the mare are reviewed. the different methods of swabbing are assessed and the criteria which should be observed when developing a swabbing technique listed. the choice of speculum and swabs available are described.1979382604
a simple method for the separation of tissue-cultured plant cells from unbound bacteria: a demonstration of acquired affinity for bacteria by cells of soy bean.tissue-cultured cells of soy bean, grown in liquid medium and then transferred to an agar substratum in so-called tissue chambers, showed an affinity for the binding of applied bacteria (klebsiella pneumoniae). this binding, which became greater as the plant cells remained on the agar for up to 10 days, was manifested as an increasing resistance of the bacteria to be washed from the plant cells in a standard, gentle and reproducible procedure devised for this purpose. the number of bacteria firm ...1979382698
neonatal lung abscess. a report of six cases.six infants 8 weeks of age or younger had lung abscesses treated in our medical center during the 20-year period of from 1957 to 1977. the duration of symptoms prior to diagnosis, the etiologic organisms, and the therapeutic approach differ from those in older children and adults. the pathogenic organisms were escherichia coli, group b streptococcus, and klebsiella pneumoniae, initial therapy with a penicillin and an aminoglycoside are indicated until identification and susceptibility results ar ...1979382838
[bactericidal kinetics of cefazolin, cefoxitin and amikacin alone and in combination (author's transl)].the in vitro activity of cefazolin, cefoxitin und amikacin alone and in combination was determined via membrane filtration against e. coli (n = 2), k. pneumoniae (n = 2) and of cefoxitin and amikacin against proteus vulgaris (n = 1). therapeutically achievable concentrations of cefazolin (10 micrograms/ml), cefoxitin (10 micrograms/ml) and amikacin (10 micrograms/ml) lead to a rapid bactericidal effect against all of our strains. the combination of cefazolin (10 micrograms/ml) and amikactin (5 m ...1978383106
hydrogen transfer in catalysis by adenosylcobalamin-dependent diol dehydratase.studies [bachovchin, w. w., et al. (1978) biochemistry 17, 2218] of the mechanism of inactivation of adenosylcobalamin-dependent diol dehydratase have led to the development of a general method to describe the kinetics of a reaction pathway containing a reservoir of mobile hydrogen. analysis by this method of catalytic rate measurements for mixtures of 1,2-propanediol and 1,1-dideuterio-1,2-propanediol supports a mechanism involving an intermediate with three equivalent hydrogens, in which hydro ...1979383139
resolution of the coenzyme b-12-dependent dehydratases of klebsiella sp. and citrobacter freundii.diol dehydratase (1,2-propanediol hydro-lyase, ec 4.2.1.28) and glycerol dehydratase (glycerol hydro-lyase, ec 4.2.1.30) are shown to be distinct, separable enzymes that occur individually or together in different strains of klebsiella sp. anaerobic growth with propan-1,2-diol induces diol dehydratase alone, whereas glycerol fermentation induces both enzymes in k. pneumoniae atcc 25955 and in citrobacter freundii ncib 3735. the dehydratases can be resolved by polyacrylamide-gel electrophoresis o ...1979383154
bacterial infection in relation to pain and irregular bleeding in women using iud.pain and metrorrhagia in women using iuds are often the reason for removing the iud. in 54 women where the iud was removed, uterine fluid for bacteriologic cultures was taken from the removed iud. there was a correlation between positive cultures and symptoms of pain and metrorrhagia. spotty bleeding caused by microtraumata and increased fibrinolytic activity of the iud might thus consitute a substrate for bacterial invasion resulting in endometritis and in turn increased bleeding and pain. in 1 ...1979383399
combined action of sulphamethoxazole and trimethoprim against clinically-isolated sulphonamide-resistant bacteria.42 strains of gram-negative bacteria, isolated from clinical material, resistant to trimethoprim (tm), sulphamethoxazole (smz) or both, have been tested for synergistic sensitivity to tm + smz. the combination showed no synergy against strains 'highly resistant' to smz (mic greater than 1 mg/ml). indifference was invariably observed. however, synergy was seen with strains 'moderately resistant' to smz (mic 100--1,000 microgram/ml). our results indicate that present techniques for the testing of ...1979383418
the coupling of electron transfer in nitrogenase to the hydrolysis of magnesium adenosine triphosphate. 1979383544
the lactose system in klebsiella aerogenes v9a. 6. lactose transport. 1979383575
comparison of assay of coliform enterotoxins by conventional techniques versus in vivo intestinal perfusion.thirty-six strains of coliform bacteria were tested for enterotoxigenicity both by conventional assays, including the y-1 adrenal and chinese hamster ovary cell assays for heat-labile toxin and the suckling mouse assay for heat-stable toxin, and by determining the ability of graded concentrations of ultrafiltrate high- or low-molecular-weight toxin preparations to induce water secretion during in vivo perfusion in the rat jejunum. the ultrafiltrates of all 18 strains isolated from persons with i ...1979383611
[experience with sisomicin in pediatrics (author's transl)].sisomicin, an aminoglycoside antibiotic, is especially effective against escherichia coli, klebsiella, enterobacter, citrobacter, serratia, indole-positive and indole-negative proteus species, pseudomonas aeruginosa, salmonella and staphylococcus aureus. it has a bactericidal action. although sisomicin is similar to the other aminoglycoside antibiotics, there is not complete cross-resistance to them. our own pharmacokinetic investigations showed that a dose of 2--3 mg/kg body weight of sisomicin ...1979383623
comparison of disc zone sizes with minimum inhibitory concentrations using semisynthetic penicillin. 1979383681
salmonella phage glycanases: substrate specificity of the phage p22 endo-rhamnosidase.interaction between phage p22 and phenol-water extracted lipopolysaccharides from sensitive salmonella bacteria belonging to serogroups a, b and di results in hydrolysis of the alpha-l-rhamnosyl linkages within the tetrasaccharide repeating unit of the o-antigenic polysaccharide chain. these o-antigens have identical structures except for the nature of the 3,6-dideoxy-hexosyl group linked to o-3 of the d-mannosyl residue. removal of the dideoxysugar, or periodate oxidation followed by borohydrid ...1979383902
the bacteriological culture of equine uterine contents, in-vitro sensitivity of organisms isolated and interpretation.a total of 19 pathogenic bacterial species was isolated from uterine swabs of 498 out of 1539 mares over 4 years. the swabs were taken by 5 veterinary clinicians using 2 different techniques. bacterial contamination during swabbing was minimized by scrupulous attention to cleansing of the external genitalia and the perineal area, and in the handling of the culture specimen. the most prevalent organisms isolated were beta-haemolytic streptococcus (39%), escherichia coli (27%) and klebsiella pneum ...1979383986
isolation of klebsiella pneumoniae from the urogenital tract of experimentally infected mares.k. pneumoniae capsule type 68 infused into the uterus of 4 mares was recovered up to 15 weeks after inoculation. the insertion of a tampon for 10 min was more effective than a swab technique in detecting the organism in the uterus. the clitoral fossa and the urethral orifice when sampled by the swab technique were also found to be infected for a comparable period. k. pneumoniae was isolated from the clitoral specimens more often and more consistently than from either urethral or uterine specimen ...1979383987
overlapping sequences of klebsiella pneumoniae nifdna cloned and characterized.a hindiii (17.0 kb) and an ecor1 restriction fragment (6.9 kb) of klebsiella pneumoniae nif dna were cloned on two small amplifiable plasmids, pcm1 and psa30 respectively. these plasmids between them carry 14 of the 15 known klebsiella nif genes. the operon for the three structural genes for nitrogenase, nifphdk, is carried on psa30: four and five of the remaining six operons are on pcra37 and pcm1 respectively. all of the nif genes were assigned to endonuclease restriction fragments of dna usin ...1979384162
[occurrence of various strains of gram-negative bacteria: yersinia, klebsiella and aeromonas and their role in the etiology of food poisoning]. 1979384496
the detection of nasal carriers of salmonella and other enterobacteria amongst young farm animals.an investigation was conducted to detect the presence of salmonella nasal carriers amongst young farm animals (cow-calves, buffalo-calves, lambs, kids, piglets and foals) on farms having a previous history of salmonellosis. salmonella could be isolated from nasal swabs of 1.2% of the cow-calves and 2.9% of the piglets studied but not from other young animals. isolated strains included s. typhimurium and s. weltevreden from cow-calves and piglets, respectively. other members of family enterobacri ...1979384721
[effects of tobacco smoke and gaseous air pollutants on the antibacterial activity of pulmonary alveolar macrophages; review of the literature]. 1979384736
comparison of ec broth and medium a-1 for the recovery of escherichia coli from frozen shucked snow crab.two variations of the multiple-tube fermentation technique were used to enumerate fecal coliforms in commercially processed, frozen crab meat. these were the ec confirmation test and a more rapid method that requires medium a-1. the method with medium a-1 was more specific than the ec confirmation test for detecting escherichia coli type 1. e. coli was isolated from 84% of the positive medium a-1 tubes, whereas it was isolated from only 64% of the positive tubes of ec broth. when samples of crab ...1979384899
[comparative study of the different media of enrichment and isolation for the identification of enteric pathogens (author's transl)].in the present study, were compared several media in use in diagnostic laboratory for the isolation of enteric pathogens. the aa. have remarked the efficiency of bacto-selenite f and of bacto-tt broth base hajna, for the enrichment; it is suggested the use of bacto agar and of bacto-hektoen enteric agar for the isolation.1979384940
cooperative binding of manganese to citrate lyase from klebsiella aerogenes. 1979385345
[in vitro antibacterial activity of fosfomycin].we have studied the "in vitro" antibacterial activity of phosphonomycin against 93 freshly isolated bacterial strains. phosphonomycin posses a good activity mainly against gram positive bacteria. a marked increase in the antibacterial activity "in vitro" is obtained when phosphonomycin is combined with other antibitoics having a similar mechanism of action (i.e. inhibition of cell-wall synthesis). we have also determined the frequency of phosphonomycin-resistant mutants in some of the strains te ...1979385409
transmissible drug resistance among klebsiella species in urinary tract infection. 1979385493
experimental gram-negative bacterial sepsis: prevention of mortality not preventable by antibiotics alone.outbred swiss mice were inoculated intraperitoneally or intravenously with one 90 to 100% lethal dose of escherichia coli o:18, proteus mirabilis, or klebsiella pneumoniae. after carefully timed intervals, aminoglycoside antibiotics were begun at dosages nnd intervals predetermined to constitute optimal therapy. with progressive increases in delay of antibiotic therapy, mortality rates increased progressively from 0% to 90 to 100%. standardized models of infection were developed by selecting del ...1979385500
klebsiella pneumonia strains moderately resistant to ampicillin and carbenicillin: characterization of a new beta-lactamase. 1979385576
intravenous trimethoprim-sulfamethoxazole in the treatment of serious infections in children.intravenous tmp-smz was used to treat 19 infectious episodes in 18 patients ranging in age from 3 weeks to 13 years. thirteen patients with various soft tissue or skeletal infections caused by haemophilus influenzae, streptococcus pneumoniae, staphylococcus aureus. streptococcus pyogenes, or acinetobacter anitratus were successfully treated. three children with four episodes of csf shunt infections due to coagulase-negative staphylococci were treated successfully also. the only treatment failure ...1979385814
predicting fatal sepsis in burn patients.the high morbidity after severe thermal insult is believed to be related partially to a resultant decrease in immunocompetence. we tested the ability of phytohemagglutinin (pha) and concanavalin (con a) to stimulate lymphocyte transformation in 17 patients with moderate to severe thermal injury (greater than 25% bsa). the patients acted as their own controls and the per cent change in their mitogen response was measured over time. eight acutely burned patients who subsequently developed severe s ...1979385911
further studies on generation of macrophages in in vitro cultures of mouse spleen cells and its inhibition by the capsular polysaccharide of klebsiella pneumoniae. 1979386053
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