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hafnia alvei strains possessing the alpha antigen of stamp and stone. 196113696727
structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1546.an acidic o-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of hafnia alvei pcm 1546 is composed of d-gal, d-glc, d-glca, d-galnac and o-acetyl groups in the ratios 1:1:1:2:1.6. on the basis of sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: [see equation in text].200314505884
[nosocomial pneumonia caused by hafnia alvei]. 200314624659
a molecular phylogeny of enteric bacteria and implications for a bacterial species concept.a molecular phylogeny for seven taxa of enteric bacteria (citrobacter freundii, enterobacter cloacae, escherichia coli, hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, and serratia plymuthica) was made from multiple isolates per taxa taken from a collection of environmental enteric bacteria. sequences from five housekeeping genes (gapa, groel, gyra, ompa, and pgi) and the 16s rrna gene were used to infer individual gene trees and were concatenated to infer a composite molecular phylogen ...200314640415
genetic and ecological structure of hafnia alvei in australia.multilocus enzyme electrophoresis of 161 hafnia alvei isolates from 158 hosts and 3 water column samples collected in australia revealed that this species consists of two genetically distinct groups. the two groups of h. alvei differed significantly in their genetic structure and host distribution. the taxonomic class of the host but not geographic locality explained a significant proportion of the observed genetic and biochemical variation among strains within each genetic group.200314666987
antimicrobial susceptibility patterns, beta-lactamases, and biochemical identification of yokenella regensburgei strains.yokenella regensburgei is an opportunistic human pathogen that phenotypically resembles hafnia alvei. the susceptibility of 10 y. regensburgei strains to 75 antimicrobial agents was examined, applying a microdilution procedure in cation-adjusted mueller-hinton broth (camhb) and isosensitest broth (isb). beta-lactamases were characterized phenotypically with beta-lactamase activity and induction assays. genotypically, pcr experiments applying degenerated primer pairs for the detection of ampc bet ...200414761716
[nosocomial hafnia alvei sepsis in a neonatal intensive care unit].hafnia alvei is a member of the enterobacteriaceae family, which is rarely associated with infection in pediatric patients and exceptionally in the neonatal period. infections caused by this organism are usually opportunistic. h. alvei bacteremias are mostly associated with abdominal disease or immunosuppression. we report four cases of nosocomial sepsis in preterm infants with positive blood cultures to h. alvei that were treated in our institution within a short period of time.200414987520
chimeric nature of two plasmids of hafnia alvei encoding the bacteriocins alveicins a and b.the complete nucleotide sequences of two bacteriocin-encoding plasmids isolated from hafnia alvei (palva and palvb) were determined. both plasmids resemble cole1-type replicons and carry mobilization genes, as well as colicin-like bacteriocin operons. these bacteriocins appear to be chimeras consisting of translocation domains from tol-dependent colicins, unique binding domains, and killing and immunity domains similar to those of the pore-forming colicin ia. just as is found for colicin ia, the ...200414996789
late-onset neonatal sepsis due to hafnia alvei.hafnia alvei infections are uncommon, and usually occur in patients with underlying illnesses, mainly adults. the authors describe a rare case of late-onset community-acquired neonatal sepsis in an infant without any underlying disease. the infant was successfully treated with cefotaxime.200415000567
structure of the o-polysaccharide from the lipopolysaccharide of hafnia alvei strain pcm 1529.the following structure of the pentasaccharide repeating unit of an acidic o-polysaccharide of hafnia alvei pcm 1529 was established by sugar and methylation analyses along with 1d and 2d 1h and 13c nmr spectroscopy: [carbohydrate structure: see text].200415013412
analysis of ampc beta-lactamase expression and sequence in biochemically atypical ceftazidime-resistant enterobacteriaceae from paediatric patients.to analyse the variation of ampc beta-lactamase gene sequence and expression in biochemically atypical enterobacteriaceae isolates, and to identify them definitively.200415014062
photobacterium phosphoreum caused a histamine fish poisoning incident.an incident of histamine fish poisoning (hfp) occurred due to the consumption of iwashi maruboshi (dried sardine) in osaka, japan in march 2002. a histamine-producing bacterial strain, ys4-7, was isolated from iwashi maruboshi that contained 1700 mg of histamine per kilogram. this strain was identified as photobacterium phosphoreum by biochemical examinations and partial sequencing of 16s rdna. p. phosphoreum ys4-7 showed greater capability as a histamine producer at 4 and 12 degrees c than morg ...200415033270
volatile compounds produced in cheese by enterobacteriaceae strains of dairy origin.the formation of volatile compounds in fresh cheese by 10 enterobacteriaceae strains of dairy origin (4 hafnia alvei, 2 serratia liquefaciens, 1 enterobacter cloacae, 1 enterobacter sakazakii, and 2 escherichia coli strains) was investigated. small cheeses were made from pasteurized cow's milk separately inoculated with 1-3 x 10(3) cfu/ml of each of the enterobacteriaceae strains, with glucono-8-lactone added to achieve a ph value of 5.2 in the curds. all strains reached counts close to 10(8) cf ...200415035375
dna based classification of food associated enterobacteriaceae previously identified by biolog gn microplates.enterobacteriaceae are frequently isolated from food products and it is essential to have methods for correct identification for both food hygiene and epidemiology reasons. phenotypic methods are not always sufficient and have to be supplemented by dna based methods. in the present study, 70 strains of enterobacteriaceae derived from milk, fish and meat that had previously been identified by biolog gn microplates were genomically classified together with 15 representative type strains of species ...200415046311
sanitary and bacteriological aspects of sewage treatment.a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ...200315058814
nucleotide sequence and expression of the ncr nickel and cobalt resistance in hafnia alvei 5-5.the structural genes for the nickel and cobalt resistance of the conjugative plasmid pejh 501 of hafnia alvei 5-5, contained on a sali-ecori fragment of 4.8 kb, were cloned and sequenced. the dna sequence included five genes in the following order: ncra, ncrb, ncrc, ncry, and ncrx. the predicted amino acid sequences of ncra were homologous to the amino acid sequences of nreb of achromobacter xylosoxidans 31a. expression of ncr with the t7 rna polymerase-promoter system allowed escherichia coli b ...200415179604
characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis.the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ...200415183874
presence of acylated homoserine lactones (ahls) and ahl-producing bacteria in meat and potential role of ahl in spoilage of meat.quorum-sensing (qs) signals (n-acyl homoserine lactones [ahls]) were extracted and detected from five commercially produced vacuum-packed meat samples. ninety-six ahl-producing bacteria were isolated, and 92 were identified as enterobacteriaceae. hafnia alvei was the most commonly identified ahl-producing bacterium. thin-layer chromatographic profiles of supernatants from six h. alvei isolates and of extracts from spoiling meat revealed that the major ahl species had an r(f) value and shape simi ...200415240313
hafnia alvei infection in pullets in italy.this paper describes an outbreak of disease caused by hafnia alvei in pullets. cloudy swelling and the fatty degeneration of liver associated with splenic lymphocytic depletion were the most preminent lesions. the organism was identified by biochemical tests and by a hafnia-specific bacteriophage test. laying hens and pullets were infected experimentally with the organism by the oral and intraperitoneal route, and the clinical and pathological effects were similar to those observed in naturally ...200415276988
detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor.biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ...200415466560
evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria.we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species.200415471997
the o-acetylation patterns in the o-antigens of hafnia alvei strains pcm 1200 and 1203, serologically closely related to pcm 1205.serological tests revealed immunochemical similarities between the lipopolysaccharides of hafnia alvei strains pcm 1200, 1203 and 1205. immunoblotting and elisa showed cross-reactions between the strains. nmr spectroscopy showed that the o-deacetylated o-specific polysaccharides isolated from lipopolysaccharides of h. alvei strains pcm 1200 and 1203 possessed the same composition and sequence as the o-deacetylated o-specific polysaccharide of h. alvei strain pcm 1205, that is a glycerol teichoic ...200415476713
antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens.the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ...200415527919
evolutionary genetics of a new pathogenic escherichia species: escherichia albertii and related shigella boydii strains.a bacterium originally described as hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic escherichia coli. subsequent studies identified similar h. alvei-like strains that are positive for an intimin gene (eae) probe and, based on dna relatedness, are classified as a distinct escherichia species, escherichia albertii. we determined sequences for multiple housekeeping genes in five e. albertii strains and c ...200515629933
[histopathologic studies in experimental uveitis].experimental uveitis is one of the main models in the diseases of autoimmunological background. the purpose of this paper was to analyze the quantitative histological changes in the experimental uveitis, induced by different types of homogenous endotoxin salts of havnia alvei. we studied 74 eyes of lewis rats (males) divided into 4 groups. each group received a homogenous salt of havnia alvei in a single subcutaneous injection. in the 1 group-lps ca++, in 2 group-lps na++, in 3 group-lps 981, in ...200415636226
structure of the o-polysaccharide of hafnia alvei strain pcm 1189 that has hexa- to octasaccharide repeating units owing to incomplete glucosylation.the o-polysaccharide of hafnia alvei pcm 1189 consists of d-glucose, d-galactose, d-galnac and d-glca and lacks the strict regularity. the intact and carboxyl-reduced polysaccharides as well as oligosaccharides obtained by partial acid hydrolysis were studied by chemical and enzymatic analyses, methylation and nmr spectroscopy. the following structure was established for the o-polysaccharide, which is built up of branched hexa- to octasaccharide repeating units differing in the number of lateral ...200515639246
spontaneous bacterial peritonitis due to hafnia alvei in a patient with peritoneal mesothelioma. 200515756379
natural antimicrobial susceptibility patterns and biochemical identification of escherichia albertii and hafnia alvei strains.bangladeshi diarrheagenic hafnia alvei-like strains have been described recently as the new species escherichia albertii (int j syst evolut microbiol. 2003;53:807-810). the natural susceptibility of 21 e. albertii and 76 h. alvei strains to 69 antimicrobial agents was examined, applying a microdilution procedure in isosensitest broth (for all the strains) and cation-adjusted mueller-hinton broth (for some strains). examining the phenotypic features of both taxa with commercial identification sys ...200515766600
structures of the biological repeating units in the o-chain polysaccharides of hafnia alvei strains having a typical lipopolysaccharide outer core region.earlier, the structures of the o-chain polysaccharides of the lipopolysaccharides (lps) of a number of hafnia alvei strains have been established. however, it remained unknown, which is the first and the last monosaccharide of the o-chain. this is defined by the structure of the so-called biological repeating unit (o-unit), which is pre-assembled and then polymerised in the course of biosynthesis of bacterial polysaccharides by the wzy-dependent pathway. now we report on the structures of the o- ...200515961298
the effect of electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on raw chicken breasts stored at four degrees celsius for fourteen days.the effect of high-energy electron beam irradiation on the survival of salmonella enterica serovar typhimurium and psychrotrophic bacteria on commercial chicken breast meat was evaluated. fresh chicken breast meat was purchased from a local poultry processor, inoculated with 8 log10 cfu/ml salmonella, packaged in styrofoam trays and over wrapped with a polyvinyl chloride film, and subjected to 0, 1, 2, or 3 kgy of irradiation. the packaged samples were stored at 4 degrees c and analyzed for salm ...200515971537
identification of two distinct hybridization groups in the genus hafnia by 16s rrna gene sequencing and phenotypic methods.a collection of 52 strains belonging to the hafnia alvei complex were subjected to molecular (16s rrna gene sequencing) and biochemical analysis. based upon 16s rrna gene sequencing results, two genetic groups were identified which correspond with previously recognized dna hybridization group 1 (atcc 13337(t) and atcc 29926; n = 23) and dna hybridization group 2 (atcc 29927; n = 29). of 46 biochemical tests used to characterize hafniae, 19 reactions (41%) yielded variable results. of these 19 te ...200516000455
[hafnia alvei in a rare case of severe pneumonia in a postanesthesia recovery unit].an 85-year-old man with a history of moderate chronic obstructive pulmonary disease in treatment with anticholinergic drugs was admitted to the recovery unit for postoperative monitoring after right inguinal herniorrhaphy surgery and removal of a strangulated intestinal section. on the third day after surgery the patient developed radiographic signs consistent with pneumonia and required mechanical ventilation. two blood cultures and a respiratory secretion sample grew a microorganism identified ...200516038176
virulence factors and pathogenicity of hafnia alvei for gilthead seabream, sparus aurata l.virulence factors (eae gene, haemolytic capacity, fimbriae, resistance to the bactericidal effect of serum, siderophore production) and pathogenicity for gilthead seabream, sparus aurata l., were analysed for 23 hafnia alvei strains. none of the strains used in ld50 studies were lethal for seabream at doses as high as >10(8) cfu ml(-1). in chronic challenge studies differences in severity of the inflammatory response were observed between strains. on the basis of correlation of the inflammatory ...200516083446
isolation of vibrio cholerae from neonates admitted to an urban diarrhoeal diseases hospital in bangladesh.very little is known about vibrio cholerae infection in neonates with diarrhoea.200516156982
abdominal abscess and hafnia alvei septicemia occurring during the aplastic phase after autologous stem-cell transplantation in a patient with diffuse large b-cell lymphoma.hafnia alvei is a motile gram-negative bacterium that is rarely isolated from human specimens, but that sometimes can be found as part of the gastrointestinal flora. here we report a rare case of hafnia alvei septicemia with an abdominal abscess in a 60-year-old woman with diffuse large b-cell lymphoma involving the spleen, liver, and then lymph nodes. she initially received a splenectomy, and, over a 2-year period, four courses of chemotherapy. after achieving complete remission status, she und ...200416163467
clinical characteristics of nosocomial and community-acquired extraintestinal infections caused by hafnia alvei.36 episodes (25 nosocomial and 11 community-acquired) of infections caused by hafnia alvei showed that this bacterium is responsible for serious infections in adults, specially in hospitalized patients with underlying chronic diseases, subjected to invasive procedures or even under antibiotic treatment.200516308222
biochemical and molecular characterization of obesumbacterium proteus, a common contaminant of brewing yeasts.we have evaluated the effectiveness of api 20e, biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (eric)-pcr to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant obesumbacterium proteus. of the five typing techniques, biolog testing, plasmid profiling, and eric-pcr provided the most differentiation, and api 20e testing and ribotyping were relatively indiscriminate. the molecular biology approach of eric-pc ...199416349260
the genus hafnia: from soup to nuts.the genus hafnia, a member of the family enterobacteriaceae, consists of gram-negative bacteria that are occasionally implicated in both intestinal and extraintestinal infections in humans. despite the fact that the genus currently contains only a single species (h. alvei), more extensive phylogenetic depth (two or more species) is apparent based upon dna relatedness and 16s rrna gene sequencing studies. hafnia causes a variety of systemic infections, including septicemia and pneumonia; however, ...200616418520
[beta-lactamases of gram negative bacteria: never-ending clockwork!].the acquired resistance against the wide-spectrum and highly stable beta-lactams including third-generation cephalosporins (3gc) and carbapenems is constinuously increasing and widespead with the discovery of various plasmid-encoded, or genes cassette or integrons coding for a novel beta-lactamase, always a major mechanism of resistance. to explain resistance against 3gc, with the continuing story with tem and shv mutated enzymes, several types of esbl (class a) emerge the ctx-m type, at least c ...200616420989
characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1.to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples.200616430517
nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems.dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ...199516535048
[bacteremia caused by hafnia alvei in an intensive care neonatal unit]. 200616650371
population-based laboratory surveillance of hafnia alvei isolates in a large canadian health region.hospital-based series have characterized hafnia alvei primarily as an infrequent agent of polymicrobial nosocomial infections in males with underlying illness.200616707023
[obstructive pyocholecystitis and septicaemia related from hafnia alvei: influence of diabetes mellitus]. 200616716560
a complex of lactoferrin with monophosphoryl lipid a is an efficient adjuvant of the humoral and cellular immune response in mice.our recent investigations demonstrated adjuvant properties of lactoferrin (lf). other studies proved efficacy and safety of monophosphoryl lipid a (mpl) as an adjuvant in humans. in an attempt to construct more efficient and safer adjuvants, we evaluated the activity of lf-mpl complex, formed by incubation of lf and mpl from hafnia alvei at 20:1 w/w ratio, and verified its characteristics by sds-page analysis. binding kinetics was determined by surface plasmon resonance analysis using a biacore ...200616838169
isolation of toxigenic hafnia alvei from a probable case of hemolytic uremic syndrome.an 11-year-old girl presented to a central california children's hospital with a 3-day history of erythematous lesions on her forehead, neck, and trunk, abdominal pain, persistent emesis, and decreased urinary output. one day prior to admission she had a mild bout of diarrhea with a small amount of blood in her stool. upon admission her condition rapidly worsened with acute renal failure, anemia, and thrombocytopenia. one of the possible causes of this condition included hemolytic uremic syndrom ...200616896583
microbiological characteristics of "androlla", a spanish traditional pork sausage.counts of total aerobic mesophilic microflora, lactic acid bacteria, salt-tolerant microflora, enterobacteriaceae, enterococci, moulds and yeasts, and staphylococci, and some physico-chemical parameters (total solids, nacl and nitrate contents and ph and aw values) were determined in 20 units of "androlla", a traditional dry-fermented sausage made in the nw of spain. in general, high counts of all the investigated microbial groups were observed, with average values of 8.99 +/- 0.46 log cfu/g for ...200716943094
genetic environment of acquired bla(acc-1) beta-lactamase gene in enterobacteriaceae isolates.we studied the genetic organization of bla(acc-1) in 14 isolates of enterobacteriaceae from france, tunisia, and germany. in a common ancestor, isecp1 was likely involved in the mobilization of this gene from the hafnia alvei chromosome to a plasmid. other genetic events involving insertion sequences (particularly is26), transposons (particularly tn1696), or suli-type integrons have occurred, leading to complex genetic environments.200616982793
increased serum iga and igm against lps of enterobacteria in chronic fatigue syndrome (cfs): indication for the involvement of gram-negative enterobacteria in the etiology of cfs and for the presence of an increased gut-intestinal permeability.there is now evidence that chronic fatigue syndrome (cfs) is accompanied by immune disorders and by increased oxidative stress. the present study has been designed in order to examine the serum concentrations of iga and igm to lps of gram-negative enterobacteria, i.e. hafnia alvei; pseudomonas aeruginosa, morganella morganii, proteus mirabilis, pseudomonas putida, citrobacter koseri, and klebsiella pneumoniae in cfs patients, patients with partial cfs and normal controls. we found that the preva ...200717007934
etiologic agents of cervicovaginitis in turkish women.to investigate the distribution of microbiologic agents causing cervicovaginitis.200617013472
misidentification of a mucoid strain of salmonella enterica serotype choleraesuis as hafnia alvei by the vitek gni+ card system.nontyphoidal salmonellae are among the most common causes of bacterial gastroenteritis worldwide. they are also notable causes of extraintestinal infections, including bacteremia and vascular infections. salmonella enterica serotype choleraesuis is typically associated with invasive infections. we report a patient who had an infected intra-abdominal aortic aneurysm due to an unusually mucoid strain of salmonella enterica serotype choleraesuis. the isolate was erroneously identified as hafnia alv ...200617050821
structure and serological analysis of the hafnia alvei 481-l o-specific polysaccharide containing phosphate in the backbone chain.the lipopolysaccharide was extracted from cells of hafnia alvei 481-l bacterial strain and, after mild acid hydrolysis, the o-specific polysaccharide was isolated and characterised. on the basis of chemical analyses and nmr spectroscopic studies of the polysaccharide and oligosaccharides obtained after smith degradation, or hydrogen fluoride treatment, it was found that the repeating unit of the o-specific polysaccharide is a phosphorylated hexasaccharide: [see text]. the biological repeating un ...200617081508
occurrence of proteolytic activity and n-acyl-homoserine lactone signals in the spoilage of aerobically chill-stored proteinaceous raw foods.proteolytic pseudomonads dominate the spoilage flora of aerobically chill-stored proteinaceous raw foods. proteolysis during spoilage of these food systems affects both food quality and the dynamics of the bacterial community because it increases the availability of nutrients to the community as a whole. quorum sensing, or cell-cell signaling, is associated closely with ecological interactions among bacteria in mixed communities. the potential role of quorum sensing in proteolytic food spoilage ...200617133818
examination of the new alpha-(2'z-fluoro)vinyl trigger with lysine decarboxylase: the absolute stereochemistry dictates the reaction course. 200717212389
hafnia alvei endocarditis following pyelonephritis in a permanent pacemaker carrier. 200717256223
[pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy].pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ...200617297889
young-infant sepsis combined with urinary tract infection due to hafnia alvei.hafnia alvei infections are uncommon and occur mainly in adult patients featuring underlying illnesses. its isolation in pediatric cases is even more unusual. we report a rare case of sepsis combined with urinary tract infection caused by h. alvei in a 39-day-old infant who did not appear to feature any underlying disease. the infant was successfully treated with ceftriaxone over a 14-day period. in this case, we want to remind clinicians that the possibility of an extraintestinal invasive infec ...200717493908
[genetic markers of pathogenicity of conditionally pathogenic enterobacteria isolated from children with diarrheal diseases].in the process of examination of 89 children from different age groups with diarrheal disease caused by bacteria from enterobacteriaceae family 89 microorganisms were isolated including klebsiella spp. (37 isolates), citrobacter spp. (9 isolates), enterobacter spp. (17 isolates), hafnia alvei (1 isolate), morganella morganii (11 isolates), proteus spp. (14 isolates). presence of genes associated with pathogenicity islands (pais): hlya, hlyb (hemolysin), sfag (fimbria antigen type s), cnf1 (cytot ...200717523471
absidia corymbifera in an immune competent accident victim with multiple abdominal injuries: case report.we report a case of mucormycosis in a healthy 17-year-old accident victim with multiple abdominal injuries which was caused by infection with absidia corymbifera, a ubiquitous saphrophyte in the ground.200717531089
failure to detect salmonella species in a population of wild tuatara (sphenodon punctatus).to assess the prevalence of faecal excretion of salmonella serovars by wild tuatara (sphenodon punctatus) on stephens island, new zealand.200717534416
evidence of temporal postdischarge decontamination of bacteria by gliding electric discharges: application to hafnia alvei.this study aimed to characterize the bacterium-destroying properties of a gliding arc plasma device during electric discharges and also under temporal postdischarge conditions (i.e., when the discharge was switched off). this phenomenon was reported for the first time in the literature in the case of the plasma destruction of microorganisms. when cells of a model bacterium, hafnia alvei, were exposed to electric discharges, followed or not followed by temporal postdischarges, the survival curves ...200717557841
first total synthesis of a pentasaccharide repeating unit of the o-antigen of hafnia alvei pcm 1529.a pentasaccharide repeating unit of the o-antigen of hafnia alvei has been synthesized in a concise manner. high yielding glycosylation steps and minimum number of protecting group manipulation steps are the key features of this synthesis. a two-step, one-pot phase transfer oxidation protocol has been applied for the preparation of d-galacturonic acid.200817659441
plasmid-encoded acc-4, an extended-spectrum cephalosporinase variant from escherichia coli.acc-4, an omega loop mutant (val(211)-->gly) of the hafnia alvei-derived cephalosporinase acc-1, was encoded by an escherichia coli plasmid. the genetic environment of bla(acc-4) shared similarities with plasmidic regions carrying bla(acc-1). kinetics of beta-lactam hydrolysis and levels of resistance to beta-lactams showed that acc-4 was more effective than acc-1 against expanded-spectrum cephalosporins.200717664321
contamination of milk by enterococci and coliforms from bovine faeces.to determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. methods: putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm. the clonal relationships of each bacterial group were investigated using pulsed-field gel electrophoresis of genomic macrorestriction fragments. representatives of the different clust ...200717953550
microbial interactions within a cheese microbial community.the interactions that occur during the ripening of smear cheeses are not well understood. yeast-yeast interactions and yeast-bacterium interactions were investigated within a microbial community composed of three yeasts and six bacteria found in cheese. the growth dynamics of this community was precisely described during the ripening of a model cheese, and the lotka-volterra model was used to evaluate species interactions. subsequently, the effects on ecosystem functioning of yeast omissions in ...200817981942
concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457.concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457 is reported. construction of the tetrasaccharide as its 4-methoxyphenyl glycoside was achieved by condensation of less abundant monosaccharide units such as, d-galactofuranose, n-acetyl-d-galactosamine and n-acetylneuraminic acid. the synthetic strategy consists of the preparation of suitably protected required monosaccharide intermediates from the commercially available reducing sugars and high ...200818074224
influence of environmental conditions on biofilm formation by hafnia alvei strains.hafnia alvei is considered an opportunistic pathogen of animal and humans, affecting a wide range of homeothermic and poikilothermic hosts with different body temperatures. in this work, h. alvei strains isolated from different sources were studied with regard to their capacity to form biofilms under different environmental conditions. strain, growth phase, temperature and culture media dependent changes of biofilm formation were semiquantitatively monitored using a microtiter plate method. our ...200818093756
method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat products.in tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ...200818155310
antibiotic resistance and adherence properties of hafnia alvei clinical isolates: a 19-month study in the hospital of orléans, france.we studied the epidemiology of antibiotic resistance and adherence properties among all hafnia alvei clinical isolates collected from august 2003 to february 2005 from patients hospitalized in the hospital of orléans, france. the isolates were typed by random amplified polymorphic dna (rapd), screened for antibiotic resistance and bacterial adherence to a549 respiratory and t24 bladder cells. six intestinal, 3 respiratory, and 8 isolates from different body sites were collected. a total of 12 ra ...200718230550
lactoferrin-monophosphoryl lipid a complex enhances immunity of mice to plesiomonas shigelloides cnctc 138/92.our previous study showed the efficacy of lactoferrin-monophosphoryl lipid a isolated from hafnia alvei lps complex (lf-mpl(h.a.)) as an adjuvant in stimulation of humoral and cellular immune response in mice to conventional antigens and a lower pyrogenicity of the complex as compared with mpl(h.a.) alone. in the present investigation we demonstrated that lf-mpl(h.a.) complex enhanced the immunity of balb/c mice immunized with plesiomonas shigelloides cnctc 138/92 bacterial vaccine, against p. s ...200818231655
[gene cloning, expression and characterization of a novel phytase from hafnia alvei].a gene appa encoding a novel phytase was firstly cloned from hafnia alvei by pcr and sequenced. the gene was consisted of 1335 bp, encoding 444 amino acids. the calculated molecular weight of the mature appa was about 45.2 kd. the gene appa was expressed in e. coli bl21 (de3). recombinant appa was purified and its enzymatic properties were determined. the optimum ph for the enzyme was 4.5 and the optimum temperature was 60 degrees c. the ph stability of r-appa is good, the relative phytase activ ...200718257229
the gut-brain barrier in major depression: intestinal mucosal dysfunction with an increased translocation of lps from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression.there is now evidence that major depression (mdd) is accompanied by an activation of the inflammatory response system (irs) and that pro-inflammatory cytokines and lipopolysacharide (lps) may induce depressive symptoms. the aim of the present study was to examine whether an increased gastrointestinal permeability with an increased translocation of lps from gram negative bacteria may play a role in the pathophysiology of mdd. toward this end, the present study examines the serum concentrations of ...200818283240
identification of a non-pathogenic surrogate organism for chlorine dioxide (clo2) gas treatment.the identification of non-pathogenic surrogate microorganisms is beneficial for determining and validating the efficacy of antimicrobial treatments in food manufacturing environments. a surrogate organism was identified to aid in the decontamination process of fresh produce when treated with chlorine dioxide (clo(2)) gas. thirty-two known strains of pathogenic and non-pathogenic microorganisms and seven unknown microbial isolates from mushroom, tomatoes, and strawberries were evaluated. the prim ...200818456115
vitek 2 failure in screening hafnia alvei inducible beta-lactam resistance. 200818511150
molecular characterization of a cryptic plasmid from the psychrotrophic antarctic bacterium pseudoalteromonas sp. 643a.we report the identification and nucleotide sequence analysis of pkw1, a plasmid of the psychrotrophic bacterium pseudoalteromonas sp. 643a isolated from the stomach of antarctic krill euphasia superba. pkw1 consists of 4583 bp, has a g+c content of 43% and seven putative open reading frames (orfs). the deduced amino acid sequence from orf-1 shared significant similarity with the plasmid replicase protein of psychrobacter cryohalolentis, strain k5. the dna region immediately downstream of the or ...200818611409
an unexpected isolate of hafnia alvei with reduced susceptibility to cefoxitin. 200818653239
[sepsis induced by hafnia alvei in a kidney transplant patient]. 200818662166
[intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees].dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ...200818683661
re: hafnia alvei infection of the deep palmar space. 200818687848
biodiversity of the microbial community in a spanish farmhouse cheese as revealed by culture-dependent and culture-independent methods.the microbial diversity within alberquilla cheese, made from a spontaneously fermented mixture of raw goats' and sheep's milk in the alpujarra mountains (granada, south-east spain), has been studied by the classical culturing method and also by molecular analysis of community dna. a collection of 206 isolates was obtained from the cheese on different selective/differential media, which were then re-grouped to 52 after randomly amplified polymorphic dna (rapd)-pcr analyses. isolates on man-rogosa ...200818692931
graft versus host disease-related hafnia alvei colonization and probable infection.we describe the case of a graft versus host disease (gvhd) patient, in whom hafnia alvei was cultured as a single organism, and at high bacterial counts from stool samples, from the onset of the disease until its resolution. this case is a further example of the contentious role of this species in causing human intestinal disease. furthermore, it focuses on enteric damage by gvhd as a risk factor for acquiring h. alvei colonization, and probably infection.200818719190
diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots.forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ...200818759227
impact on disinfection efficiency of cell load and of planktonic/adherent/detached state: case of hafnia alvei inactivation by plasma activated water.this paper describes the effects of initial microbial concentration and planktonic/adherent/detached states on the efficiency of plasma-activated water. this disinfecting solution was obtained by treating distilled water with an atmospheric pressure plasma produced by gliding electric discharges in humid air. the inactivation kinetics of planktonic cells of hafnia alvei (selected as a bacterial model) were found to be of the first order. they were influenced by the initial microbial concentratio ...200818769918
invasion and intracellular survival of hafnia alvei strains in human epithelial cells.the aim of this study was to investigate the invasion and intracellular survival of different hafnia alvei strains in hela cells.200818795976
structure of the glycerol phosphate-containing o-specific polysaccharide and serological studies on the lipopolysaccharides of citrobacter werkmanii pcm 1548 and pcm 1549 (serogroup o14).the o-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of citrobacter werkmanii pcm 1548 and pcm 1549 (serogroup o14) and found to contain d-glucose, d-glucosamine and glycerol-1-phosphate in molar ratios 2 : 2 : 1. based on methylation analysis and 1h and 13c nuclear magnetic resonance spectroscopy data, it was established that the o-specific polysaccharides from both strains have the identical branched tetrasaccharide repeating unit with 3,6-disubstituted ...200818811720
immunochemical studies of the lipopolysaccharides of hafnia alvei pcm 1219 and other strains with the o-antigens containing d-glucose 1-phosphate and 2-deoxy-2-[(r)-3-hydroxybutyramido]-d-glucose.hafnia alveiis the only species of the genus hafnia, which belongs to the family of enterobacteriaceae. these gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. their lipopolysaccharides (lpss) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. so far, 29 different o-polysaccharide (ops, o-antigen) structures in hafni ...200818836891
[the analysis of the bacterial and fungal flora in maxillary sinuses in patients operated due to fess method].the aim of this work was to analyse the bacterial and fungal flora in maxillary sinuses in patients operated due to the fess method.200818837224
escherichia albertii and hafnia alvei are candidate enteric pathogens with divergent effects on intercellular tight junctions.attaching-effacing lesion-inducing escherichia albertii and the related, but non-attaching-effacing organism, hafnia alvei, are both implicated as enteric pathogens in humans. however, effects of these bacteria on epithelial cells are not well-characterized. related enteropathogens, including enterohemorrhagic escherichia coli o157:h7, decrease epithelial barrier function by disrupting intercellular tight junctions in polarized epithelia. therefore, this study assessed epithelial barrier functio ...200818930803
two kdo-heptose regions identified in hafnia alvei 32 lipopolysaccharide: the complete core structure and serological screening of different hafnia o serotypes.hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. various 3-deoxy-d-manno-oct-2-ulosonic acid (kdo)-containing fragments different from known structures of core oligosaccharides were previously found among fractions obtained by mild acid hydrolysis of some h. alvei lipopolysaccharides (lpss). however, the positions of these segments in the lps structure were not known. analysis of de- ...200919011031
temperature influences the expression of fimbriae and flagella in hafnia alvei strains: an immunofluorescence study.hafnia alvei, a gram negative bacillus related to the enterobacteriaceae family, is considered an opportunistic pathogen of several animal species and humans. in this communication, we describe fimbrial-like structures from different strains of h. alvei that cannot be easily ascribed to any of the previously reported fimbrial types in this species (type i or type iii). polymerase chain reaction (pcr) and immunofluorescence assays were carried out to study fimbriae and flagella in h. alvei strain ...200919011836
normalization of leaky gut in chronic fatigue syndrome (cfs) is accompanied by a clinical improvement: effects of age, duration of illness and the translocation of lps from gram-negative bacteria.there is now evidence that an increased translocation of lps from gram negative bacteria with subsequent gut-derived inflammation, i.e. induction of systemic inflammation and oxidative & nitrosative stress (io&ns), is a new pathway in chronic fatigue syndrome (cfs).200819112401
isolation of colistin-resistant hafnia alvei. 200919141752
microbial inactivation using plasma-activated water obtained by gliding electric discharges.to evaluate the microbial disinfection efficacy of a plasmachemical solution obtained by the activation of water with gliding electric discharges.200919170858
molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk.the aim of this study was to use molecular techniques to assess the microbiota of eight raw cow's milk samples at biotype and species level. sixty-six isolates from raw milk samples were screened by randomly amplified polymorphic dna-pcr (rapd-pcr) biotyping and representative strains of rapd-pcr profiles were identified by 16s rrna gene sequencing. pseudomonas spp. were the most commonly occurring contaminants along with enterobacteriaceae such as hafnia alvei, serratia marcescens and citrobact ...200919171267
isolation of hafnia species from kimchi.eight commercially packaged kimchi products were examined over 15 days of storage at 4 degrees to evaluate the occurrence of hafnia alvei (h. alvei). additionally, 7 saeujeot products, as a possible ingredient source, were analyzed to examine the bacteria's origin. over the storage period, kimchi samples had decreasing ph levels, which stabilized at ph 4.2; acidity increased to 0.9+/-0.1%. lactose-nonfermenting bacteria, which h. alvei belongs to, gradually reduced in numbers over the kimchi sto ...200919190412
[taxonomic characteristics and physiological properties of microorganisms from the gut of pike (esox lucius)].the taxonomic composition and distribution of microorganisms differing in the degree of association with the intestinal mucosa of the pike (lucius lucius) has been studied. microorgansism of the families enterobacteriaceae, aeromonadaceae, and vibrionaceae dominate in the gut microflora. numerically prevailing bacterial species are characterized by high proteolytic and amylolytic enzyme activities as well as by high persistence accounted for by antilysozyme and antihistone activities. the result ...200819198074
comparison of pcr-based molecular markers for the characterization of proteus mirabilis clinical isolates.proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. for epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (pfge) or ribotyping, have been developed for this pathogen. however, these methods are labor intensive a ...200819219283
polyphasic study of microbial communities of two spanish farmhouse goats' milk cheeses from sierra de aracena.the microbial communities present in 2 different types of farmhouse goats' milk cheese from the aracena mountains (southwest spain), quesailla arochena (hard cheese) and torta arochena (soft cheese), have been studied using both culture-dependent and culture-independent techniques. all bacterial isolates were clustered by using randomly amplified polymorphic dna (rapd) and identified by 16s rrna gene sequencing, species-specific pcr and multiplex pcr. thus a total of 26 different species were id ...200919269572
[aberrant gallstones--extraordinary complications of laparoscopic cholecystectomy].about 0.2 % of patients undergoing laparoscopic cholecystetomy will suffer from complications caused by lost gallstones. diagnostic and therapeutic measures are correlated to the symptoms. at different locations, abscesses can arise, which can be treated by direct access through the -abdominal wall, laparotomy or laparoscopy. two cases are presented with the especially grave sequels of subphrenic abscess, infiltration of the thoracic wall, and pleural empyema, which -respectively needed several ...200919382051
clostridium perfringens liver abscess after pancreatic resection.liver abscess is a rare complication after pancreatectomy. clostridium perfingens is a rare cause of intrahepatic infections.200919388837
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