Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a case report of citrobacter koseri bacteraemia after transfusion of contaminated red cells. | citrobacter koseri has not been associated to infection caused by blood component transfusions. | 2012 | 23035958 |
| cellulolytic bacteria in the foregut of the dromedary camel (camelus dromedarius). | foregut digesta from five feral dromedary camels were inoculated into three different enrichment media: cotton thread, filter paper, and neutral detergent fiber. a total of 283 16s rrna gene sequences were assigned to 33 operational taxonomic units by using 99% species-level identity. libshuff revealed significant differences in the community composition across all three libraries. | 2012 | 23042173 |
| comparison of pcr/electron spray ionization-time-of-flight-mass spectrometry versus traditional clinical microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers. | understanding nosocomial pathogen transmission is restricted by culture limitations. novel platforms, such as pcr-based electron spray ionization-time-of-flight-mass spectrometry (esi-tof-ms), may be useful as investigational tools. | 2012 | 23050585 |
| resistance to antibiotics in urinopathogenic bacteria isolated in patients attending kenyatta university health clinic, nairobi. | to determine the aetiological bacterial agents of urinary tract infections, within communities in kenyatta university, and current resistance levels to commonly available therapeutic agents. | 2010 | 23057307 |
| molecular identification and detection of virulence genes among pseudomonas aeruginosa isolated from different infectious origins. | pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity. the aim of this study was to evaluate opri, oprl and toxa genes for pcr identification of clinical p. aeruginosa. in order to find out any relation between special virulence factors and special manifestation of p. aeruginosa infections, we detected virulence factors among these isolates by pcr. ribotyping was used to evaluate the clonal relationship between strains with the same genetic patt ... | 2012 | 23066485 |
| human monocytes differentiate into dendritic cells subsets that induce anergic and regulatory t cells in sepsis. | sepsis is a multifactorial pathology with high susceptibility to secondary infections. innate and adaptive immunity are affected in sepsis, including monocyte deactivation. | 2012 | 23071758 |
| citrobacter koseri bacteraemia complicated by paraspinal abscess and spondylodiscitis--a case report. | paraspinal abscess and spondylodiscitis due to citrobacter koseri is a very rare condition. we report a remarkable case of citrobacter koseri bacteraemia complicated by paraspinal abscess and spondylodiscitis in a patient who has successfully been treated in our hospital. our patient demonstrates one of the common challenges in the practice of infectious disease medicine, wherein an innocuous presentation may and often underlie a serious infection. this case report elucidates to us that the diag ... | 2012 | 23082432 |
| multiple antibiotic resistance indexing of coliforms to identify high risk contamination sites in aquatic environment. | bacteriological analysis of the water samples collected from upstream, midstream and downstream points along the bank of the river revealed high populations of escherichia coli, citrobacter freundii, citrobacter diversus, enterobacter aerogens and klebsiella species. all these isolates were screened against eight antibiotics to determine the prevalence of multiple antibiotic resistance among isolates at different sites of the river. the study revealed that multiple antibiotic resistance was prom ... | 2010 | 23100831 |
| microbial manipulation of the amyloid fold. | microbial biofilms are encased in a protein, dna, and polysaccharide matrix that protects the community, promotes interactions with the environment, and helps cells adhere together. the protein component of these matrices is often a remarkably stable, β-sheet-rich polymer called amyloid. amyloids form ordered, self-templating fibers that are highly aggregative, making them a valuable biofilm component. some eukaryotic proteins inappropriately adopt the amyloid fold, and these misfolded protein a ... | 2012 | 23108148 |
| multiplex pcr assay targeting a diguanylate cyclase-encoding gene, cgca, to differentiate species within the genus cronobacter. | in a comparison to the widely used cronobacter rpob pcr assay, a highly specific multiplexed pcr assay based on cgca, a diguanylate cyclase gene, that identified all of the targeted six species among 305 cronobacter isolates was designed. this assay will be a valuable tool for identifying suspected cronobacter isolates from food-borne investigations. | 2013 | 23144142 |
| a randomized trial of 7-day doripenem versus 10-day imipenem-cilastatin for ventilator-associated pneumonia. | the aim of this study was to compare a 7-day course of doripenem to a 10-day course of imipenem-cilastatin for ventilator-associated pneumonia (vap) due to gram-negative bacteria. | 2012 | 23148736 |
| detection of campylobacter species and arcobacter butzleri in stool samples by use of real-time multiplex pcr. | the presence of campylobacter (or campylobacter-like) species in stools from patients suspected of infectious gastroenteritis (n = 493) was investigated using real-time pcr for detection of arcobacter butzleri (hsp60 gene), campylobacter coli (ceue gene), campylobacter jejuni (mapa), five acknowledged pathogenic campylobacter spp. (c16s_lund assay), and the campylobacter genus (c16s_lvi assay). in total, 71.4% of the samples were positive for campylobacter dna (n = 352) by a campylobacter genus- ... | 2013 | 23152553 |
| insights into the emergent bacterial pathogen cronobacter spp., generated by multilocus sequence typing and analysis. | cronobacter spp. (previously known as enterobacter sakazakii) is a bacterial pathogen affecting all age groups, with particularly severe clinical complications in neonates and infants. one recognized route of infection being the consumption of contaminated infant formula. as a recently recognized bacterial pathogen of considerable importance and regulatory control, appropriate detection, and identification schemes are required. the application of multilocus sequence typing (mlst) and analysis (m ... | 2012 | 23189075 |
| surprises from an unusual clc homolog. | the chloride channel (clc) family is distinctive in that some members are cl(-) ion channels and others are cl(-)/h(+) antiporters. the molecular mechanism that couples h(+) and cl(-) transport in the antiporters remains unknown. our characterization of a novel bacterial homolog from citrobacter koseri, clc-ck2, has yielded surprising discoveries about the requirements for both cl(-) and h(+) transport in clc proteins. first, even though clc-ck2 lacks conserved amino acids near the cl(-)-binding ... | 2012 | 23199933 |
| ethanolamine utilization in vibrio alginolyticus. | ethanolamine is used as an energy source by phylogenetically diverse bacteria including pathogens, by the concerted action of proteins from the eut-operon. previous studies have revealed the presence of eutbc genes encoding ethanolamine-ammonia lyase, a key enzyme that breaks ethanolamine into acetaldehyde and ammonia, in about 100 bacterial genomes including members of gamma-proteobacteria. however, ethanolamine utilization has not been reported for any member of the vibrio genus. our comparati ... | 2012 | 23234435 |
| experimental and natural infections in myd88- and irak-4-deficient mice and humans. | most toll-like-receptors (tlrs) and interleukin-1 receptors (il-1rs) signal via myeloid differentiation primary response 88 (myd88) and interleukin-1 receptor-associated kinase 4 (irak-4). the combined roles of these two receptor families in the course of experimental infections have been assessed in myd88- and irak-4-deficient mice for almost fifteen years. these animals have been shown to be susceptible to 46 pathogens: 27 bacteria, eight viruses, seven parasites, and four fungi. humans with i ... | 2012 | 23255009 |
| intestinal enterobacteriaceae and escherichia coli populations in japanese adults demonstrated by the reverse transcription-quantitative pcr and the clone library analyses. | a primer set specific for escherichia coli/shigella 16s rrna was developed and used for rt-qpcr analysis of fecal samples from 35 healthy adult volunteers in combination with the previously reported primer set specific for enterobacteriaceae. enterobacteriaceae and e. coli were present in the 29 out of 35 volunteers tested as intestinal commensals at the average population levels of 10(7.1±0.9) and 10(6.8±0.7)cellsg(-1) of stools (mean±standard deviation), respectively. among the 7 volunteers, t ... | 2013 | 23262033 |
| admission surveillance cultures among patients admitted to intensive care unit. | 2012 | 23272308 | |
| whole-genome sequencing and identification of morganella morganii kt pathogenicity-related genes. | the opportunistic enterobacterium, morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at changhua christian hospital, taiwan. the kt strain of m. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. m. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of th ... | 2012 | 23282187 |
| flagella from five cronobacter species induce pro-inflammatory cytokines in macrophage derivatives from human monocytes. | cronobacter spp. are opportunistic pathogens linked to lie-threatening infections in neonates and contaminated powdered infant formula that has been epidemiologically associated with these cases. clinical symptoms of cronobacter include necrotizing enterocolitis, bacteremia, and meningitis. flagella from c. sakazakii are involved in biofilm formation and its adhesion to epithelial cells. we investigated the role of flagella from c. sakazakii st1 and st4, c. malonaticus, c. muytjensii, c. turicen ... | 2012 | 23284883 |
| mass spectrometry sequencing of transfer ribonucleic acids by the comparative analysis of rna digests (card) approach. | the comparative analysis of ribonucleic acid digests (card) approach for sequencing of transfer ribonucleic acids (trnas) is described. this method is enabled by the differential labeling of two trna populations. a set of reference trnas, whose complete sequences including modifications are known, are labeled with (16)o during enzymatic digestion. the second (candidate) set of trnas, whose sequence information is desired, is labeled with (18)o. by combining the two digests, digestion products th ... | 2013 | 23295341 |
| rectal swabs are suitable for quantifying the carriage load of kpc-producing carbapenem-resistant enterobacteriaceae. | it is more convenient and practical to collect rectal swabs than stool specimens to study carriage of colon pathogens. in this study, we examined the ability to use rectal swabs rather than stool specimens to quantify klebsiella pneumoniae carbapenemase (kpc)-producing carbapenem-resistant enterobacteriaceae (cre). we used a quantitative real-time pcr (qpcr) assay to determine the concentration of the bla(kpc) gene relative to the concentration of 16s rrna genes and a quantitative culture-based ... | 2013 | 23295937 |
| a genomic day in the life of a clinical microbiology laboratory. | next-generation sequencing technology is available to many clinical laboratories; however, it is not yet widely used in routine microbiology practice. to demonstrate the feasibility of using whole-genome sequencing in a routine clinical microbiology workflow, we sequenced the genome of every organism isolated in our laboratory for 1 day. | 2013 | 23345298 |
| iron induces bimodal population development by escherichia coli. | bacterial biofilm formation is a complex developmental process involving cellular differentiation and the formation of intricate 3d structures. here we demonstrate that exposure to ferric chloride triggers rugose biofilm formation by the uropathogenic escherichia coli strain uti89 and by enteric bacteria citrobacter koseri and salmonella enterica serovar typhimurium. two unique and separable cellular populations emerge in iron-triggered, rugose biofilms. bacteria at the air-biofilm interface exp ... | 2013 | 23359678 |
| antibiotic rotation for febrile neutropenic patients with hematological malignancies: clinical significance of antibiotic heterogeneity. | our unit adopted the single administration of cefepime as the initial treatment for febrile episodes in neutropenic patients with hematological malignancies. however, recently, cefepime-resistant gram-negative bacteremia, including those with extended-spectrum β-lactamase (esbl)-producers, was frequently observed in these patients. therefore, we instituted a rotation of primary antibiotics for febrile neutropenic patients in an attempt to control antibiotic resistance. | 2013 | 23372683 |
| bacteriology of normal frontal sinuses. | the aim of this cross-sectional trial was to identify the bacterial flora and to quantify the level of bacterial presence in healthy adult frontal sinus cavities. | 2016 | 23375589 |
| antimicrobial activity of ceftaroline-avibactam tested against clinical isolates collected from u.s. medical centers in 2010-2011. | ceftaroline-avibactam and comparator agents were tested by the broth microdilution method against 20,089 isolates consecutively collected in 2010 and 2011 from 75 u.s. medical centers. ceftaroline-avibactam was active against enterobacteriaceae (4,908 strains; mic90, 0.25 μg/ml; highest mic, 4 μg/ml), including meropenem-nonsusceptible klebsiella spp. and ceftazidime-nonsusceptible enterobacter cloacae strains (mic90, 1 μg/ml for both). ceftaroline-avibactam was also active against ceftriaxone-n ... | 2013 | 23380730 |
| bacterial colonization and resistance patterns in 133 patients undergoing a primary hip- or knee replacement in southern sweden. | prosthetic joint infections can be caused by bacteria derived from the patient's skin. the aim of the study was: (1) to determine which bacteria colonize the nose and groin in patients planned for primary hip or knee arthroplasty, (2) to determine the antimicrobial resistance patterns, and (3) to monitor changes in bacterial colonization and resistance patterns connected to surgery. | 2013 | 23409844 |
| efficacy of a marine bacterial nuclease against biofilm forming microorganisms isolated from chronic rhinosinusitis. | the persistent colonization of paranasal sinus mucosa by microbial biofilms is a major factor in the pathogenesis of chronic rhinosinusitis (crs). control of microorganisms within biofilms is hampered by the presence of viscous extracellular polymers of host or microbial origin, including nucleic acids. the aim of this study was to investigate the role of extracellular dna in biofilm formation by bacteria associated with crs. | 2013 | 23441151 |
| pcr-reverse blot hybridization assay for screening and identification of pathogens in sepsis. | rapid and accurate identification of the pathogens involved in bloodstream infections is crucial for the prompt initiation of appropriate therapy, as this can decrease morbidity and mortality rates. a pcr-reverse blot hybridization assay for sepsis, the reverse blot hybridization assay (reba) sepsis-id test, was developed; it uses pan-probes to distinguish gram-positive and -negative bacteria and fungi. in addition, the assay was designed to identify bacteria and fungi using six genus-specific a ... | 2013 | 23447637 |
| staphylococcus aureus- the predominant pathogen in the neonatal icu of a tertiary care hospital in amritsar, india. | background: an early treatment and the appropriate and the rational use of antibiotics would minimize the risk of severe morbidity and mortality in neonatal sepsis, and reduce the emergence of multi-drug resistant organisms in intensive care units. for the success of an early empiric treatment, a periodic review of the cases to assess any changing trends in the infecting organisms and their antimicrobial susceptibility is important. | 2012 | 23450439 |
| staphylococcus aureus- the predominant pathogen in the neonatal icu of a tertiary care hospital in amritsar, india. | background: an early treatment and the appropriate and the rational use of antibiotics would minimize the risk of severe morbidity and mortality in neonatal sepsis, and reduce the emergence of multi-drug resistant organisms in intensive care units. for the success of an early empiric treatment, a periodic review of the cases to assess any changing trends in the infecting organisms and their antimicrobial susceptibility is important. | 2012 | 23450439 |
| downregulation of yidc in escherichia coli by antisense rna expression results in sensitization to antibacterial essential oils eugenol and carvacrol. | the rising drug resistance in pathogenic bacteria and inefficiency of current antibiotics to meet clinical requirements has augmented the need to establish new and innovative approaches for antibacterial drug discovery involving identification of novel antibacterial targets and inhibitors. being obligatory for bacterial growth, essential gene products are considered vital as drug targets. the bacterial protein yidc is highly conserved among pathogens and is essential for membrane protein inserti ... | 2013 | 23469191 |
| multilaboratory study of the biomic automated well-reading instrument versus microscan walkaway for reading microscan antimicrobial susceptibility and identification panels. | this study compared the biomic automated well reader results to the microscan walkaway results for reading microscan antimicrobial susceptibility and identification panels at four different sites. routine fresh clinical isolates and quality control (qc) organisms were tested at each study site. a total of 46,176 microscan panel drug-organism combinations were read. the biomic category agreement for 3,117 gram-negative bacteria was 98.4%, with 1.4% minor and 0.2% major discrepancies. the biomic c ... | 2013 | 23486713 |
| prevalence of urinary colonization by extended spectrum-beta-lactamase enterobacteriaceae among catheterised inpatients in italian long term care facilities. | long term care facilities (ltcfs) play a key role in guaranteeing care to patients in developed countries. many patients, mostly elderly, access ltcfs at some time in their lives, and their healthcare pathways often require them to move back and forth between hospital and outpatient settings. these patterns bring about new challenges regarding infection control, especially healthcare associated infections. | 2013 | 23497323 |
| citrobacter koseri: an unusual cause of pyogenic liver abscess. | liver abscess is a common pathology in the indian subcontinent and usually results from amoebic or bacterial infection. pyogenic abscesses usually occur in those with underlying predisposing factors like intra-abdominal infections, biliary infections or comorbidities like malignancy, immunosuppression, diabetes mellitus and previous biliary surgery or interventional endoscopy. citrobacter is an unusual cause of pyogenic liver abscess and may occur in the setting of underlying comorbidities. we r ... | 2013 | 23505286 |
| nosocomial infections after severe trauma are associated with lower apolipoproteins b and aii. | infection after severe trauma is a significant cause of morbidity and mortality days to weeks after the initial injury. apolipoproteins play important roles in host defense and circulating concentrations are altered by the acute inflammatory response. the purpose of this study was to determine if patients who acquire infection after severe trauma have significantly lower apolipoprotein levels than trauma patients who do not become infected. | 2013 | 23511146 |
| outcomes in uk patients with hospital-acquired bacteraemia and the risk of catheter-associated urinary tract infections. | there is lack of contemporary outcome data on patients with hospital-acquired infections that cause bacteraemia. we determined the risk factors for 7-day mortality and investigated the hypothesis that, compared with central venous catheter (cvc)-associated bacteraemic infections, catheter-associated bacteraemic urinary tract infections (utis) were significantly associated with 7-day mortality. | 2013 | 23520064 |
| population pharmacokinetics and pharmacodynamics of sitafloxacin in patients with community-acquired respiratory tract infections. | an optimal dosage regimen of sitafloxacin was considered based on a pharmacokinetics and pharmacodynamics (pk-pd) analysis in patients with community-acquired respiratory tract infections (rti). a population pharmacokinetic analysis of sitafloxacin was conducted using clinical data of five clinical pharmacology studies and one clinical pk-pd study in patients with rtis. the pharmacokinetic parameters in individual patients were estimated by the bayesian method to examine any correlation between ... | 2013 | 23529500 |
| characterization of qnrb-like genes in citrobacter species of the american type culture collection. | among five american type culture collection (atcc) citrobacter strains, qnrb60 in citrobacter freundii atcc 6879, an isolate from the preantibiotic era, and qnrb61 in citrobacter braakii atcc 51113(t), a type strain belonging to the c. freundii complex, were identified. meanwhile, a truncated qnrb-like pseudogene was identified in c. freundii atcc 8090(t) and atcc 43864. no qnrb-like sequence was found in citrobacter koseri atcc 27028(t). these findings underscore the close relationship between ... | 2013 | 23529729 |
| lower respiratory tract infections (ltris): an insight into the prevalence and the antibiogram of the gram negative, respiratory, bacterial agents. | community acquired respiratory tract infections are one of the commonest health issues globally, which demand frequent visits to the family practitioners. the emergence of antibiotic resistance in the frequently isolated pathogens has complicated the use of the empiric therapy with traditional agents. | 2013 | 23543819 |
| development of a broad-range 23s rdna real-time pcr assay for the detection and quantification of pathogenic bacteria in human whole blood and plasma specimens. | molecular methods are important tools in the diagnosis of bloodstream bacterial infections, in particular in patients treated with antimicrobial therapy, due to their quick turn-around time. here we describe a new broad-range real-time pcr targeting the 23s rdna gene and capable to detect as low as 10 plasmid copies per reaction of targeted bacterial 23s rdna gene. two commercially available dna extraction kits were evaluated to assess their efficiency for the extraction of plasma and whole bloo ... | 2013 | 23586027 |
| prevalence of porphyromonas gingivalis four rag locus genotypes in patients of orthodontic gingivitis and periodontitis. | porphyromonas gingivalis is considered as a major etiological agent in periodontal diseases and implied to result in gingival inflammation under orthodontic appliance. rag locus is a pathogenicity island found in porphyromonas gingivalis. four rag locus variants are different in pathogenicity of porphyromonas gingivalis. moreover, there are different racial and geographic differences in distribution of rag locus genotypes. in this study, we assessed the prevalence of porphyromonas gingivalis and ... | 2013 | 23593379 |
| multidrug resistant citrobacter: an unusual cause of liver abscess. | liver abscesses are infectious, space occupying lesions in the liver, the two most common abscesses being pyogenic and amoebic. a pyogenic liver abscess (pla) is a rare condition with a reported incidence of 20 per 100 000 hospital admissions in the western population. the right lobe of the liver is the most common site in both types of liver abscess. clinical presentation is elusive with complaints of fever, right upper quadrant pain in the abdomen and hepatomegaly with or without jaundice. the ... | 2013 | 23608848 |
| chicken cathelicidins display antimicrobial activity against multiresistant bacteria without inducing strong resistance. | the increased prevalence of multidrug-resistant (mdr) bacteria in combination with the relatively limited development of new antibiotics presents a serious threat to public health. in chicken, especially extended-spectrum ß-lactamase (esbl) carrying enterobacteriaceae are often asymptomatically present but can infect humans. due to their broad range antimicrobial activity cathelicidins and other host defence peptides, are considered to be an attractive alternative to conventional antibiotics. in ... | 2013 | 23613986 |
| rapid diagnosis of bloodstream infections with pcr followed by mass spectrometry. | achieving a rapid microbiological diagnosis is crucial for decreasing morbidity and mortality of patients with a bloodstream infection, as it leads to the administration of an appropriate empiric antimicrobial therapy. molecular methods may offer a rapid alternative to conventional microbiological diagnosis involving blood culture. in this study, the performance of a new technology that uses broad-spectrum pcr coupled with mass spectrometry (pcr/esi-ms) was evaluated for the detection of microor ... | 2013 | 23626775 |
| dissecting the metal selectivity of merr monovalent metal ion sensors in salmonella. | two homologous transcription factors, cuer and gols, that belong to the merr metalloregulatory family are responsible for salmonella cu and au sensing and resistance, respectively. they share similarities not only in their sequences, but also in their target transcription binding sites. while cuer responds similarly to au, ag, or cu to induce the expression of its target genes, gols shows higher activation by au than by ag or cu. we showed that the ability of gols to distinguish au from cu resid ... | 2013 | 23645605 |
| sialic acid utilization by cronobacter sakazakii. | the cronobacter genus is composed of seven species, and can cause infections in all age groups. of particular concern is c. sakazakii, as this species is strongly associated with severe and often fatal cases of necrotizing enterocolitis and meningitis in neonates and infants. whole genome sequencing has revealed that the nanakt gene cluster required for the utilisation of exogenous sialic acid is unique to the c. sakazakii species (esa_03609-13).sialic acid is found in breast milk, infant formul ... | 2013 | 23706082 |
| inferring ancient metabolism using ancestral core metabolic models of enterobacteria. | enterobacteriaceae diversified from an ancestral lineage ~300-500 million years ago (mya) into a wide variety of free-living and host-associated lifestyles. nutrient availability varies across niches, and evolution of metabolic networks likely played a key role in adaptation. | 2013 | 23758866 |
| epidemiological study of hospital-acquired bacterial conjunctivitis in a level iii neonatal unit. | conjunctivitis is one of the most frequently occurring hospital-acquired infections among neonates, although it is less studied than potentially life-threatening infections, such as sepsis and pneumonia. | 2013 | 23766676 |
| potential usefulness of fdg pet/ct in patients with sepsis of unknown origin. | the role of fdg pet in the evaluation of patients with sepsis of unknown origin remains unclear. we sought to assess the value of fdg pet/ct in patients with sepsis of unknown cause and to define its priority in this group of subjects. | 2013 | 23776621 |
| [bacterial ecology of nosocomial infection in intensive care unit of laquintinie hospital douala, cameroon]. | 2013 | 23785545 | |
| identification of enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the vitek ms system. | this multicenter study evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry identifications from the vitek ms system (biomérieux, marcy l'etoile, france) for enterobacteriaceae typically encountered in the clinical laboratory. enterobacteriaceae isolates (n = 965) representing 17 genera and 40 species were analyzed on the vitek ms system (database v2.0), in accordance with the manufacturer's instructions. colony growth (≤72 h) was app ... | 2013 | 23818163 |
| genetic characteristics of ctx-m-type extended-spectrum-β-lactamase (esbl)-producing enterobacteriaceae involved in mastitis cases on japanese dairy farms, 2007 to 2011. | sixty-five ctx-m-2/15/14 extended-spectrum-β-lactamase-producing enterobacteriaceae were isolated from 258,888 mastitic milk samples from japanese dairy farms between 2007 and 2011. ctx-m-2-producing klebsiella pneumoniae and ctx-m-15-producing escherichia coli were the predominant strains isolated. there was no predominant clonal type, and clonal diversity was found even in strains isolated from a single farm. | 2013 | 23843488 |
| storage of bronchoalveolar lavage fluid and accuracy of microbiologic diagnostics in the icu: a prospective observational study. | early initiation of appropriate antimicrobial treatment is a cornerstone in managing pneumonia. because microbiologic processing may not be available around the clock, optimal storage of specimens is essential for accurate microbiologic identification of pathogenetic bacteria. the aim of our study was to determine the accuracy of two commonly used storage approaches for delayed processing of bronchoalveolar lavage in critically ill patients with suspected pneumonia. | 2013 | 23844796 |
| interplay between siderophores and colibactin genotoxin biosynthetic pathways in escherichia coli. | in escherichia coli, the biosynthetic pathways of several small iron-scavenging molecules known as siderophores (enterobactin, salmochelins and yersiniabactin) and of a genotoxin (colibactin) are known to require a 4'-phosphopantetheinyl transferase (pptase). only two pptases have been clearly identified: entd and clba. the gene coding for entd is part of the core genome of e. coli, whereas clba is encoded on the pks pathogenicity island which codes for colibactin. interestingly, the pks island ... | 2013 | 23853582 |
| risk factors for infection with multidrug-resistant bacteria in non-ventilated patients with hospital-acquired pneumonia. | to identify risk factors for the development of hospital-acquired pneumonia (hap) caused by multidrug-resistant (mdr) bacteria in non-ventilated patients. | 2013 | 23857697 |
| a riboswitch-regulated antisense rna in listeria monocytogenes. | riboswitches are ligand-binding elements located in 5' untranslated regions of messenger rnas, which regulate expression of downstream genes. in listeria monocytogenes, a vitamin b12-binding (b12) riboswitch was identified, not upstream of a gene but downstream, and antisense to the adjacent gene, pocr, suggesting it might regulate pocr in a nonclassical manner. in salmonella enterica, pocr is a transcription factor that is activated by 1,2-propanediol, and subsequently activates expression of t ... | 2013 | 23878253 |
| impact of tongue biofilm removal on mechanically ventilated patients. | to evaluate the effectiveness of a tongue cleaner in the removal of tongue biofilm in mechanically ventilated patients. | 2013 | 23887759 |
| the microbiological profile of lacrimal abscess: two decades of experience from a tertiary eye care center. | the aim of this study is to exclusively report the microbiological spectrum of lacrimal abscess and the antibiotic sensitivity patterns of the organisms. retrospective interventional study on 112 eyes of 112 patients who presented to the ophthalmic plastic clinic of a tertiary eye care center over a period of 23 years from january 1990 to february 2013 with lacrimal abscess were reviewed for demographic and microbiological profile. the culture results, organisms isolated, and their antibiotic se ... | 2013 | 23889760 |
| an rpos-dependent srna regulates the expression of a chaperone involved in protein folding. | small noncoding rnas (srnas) are usually expressed in the cell to face a variety of stresses. in this report we disclose the first target for sral (also known as ryja), a srna present in many bacteria, which is highly induced in stationary phase. we also demonstrate that this srna is directly transcribed by the major stress σ factor σ(s) (rpos) in salmonella enterica serovar typhimurium. we show that sral srna down-regulates the expression of the chaperone trigger factor (tf), encoded by the tig ... | 2013 | 23893734 |
| reverse transcription-pcr-electrospray ionization mass spectrometry for rapid detection of biothreat and common respiratory pathogens. | electrospray ionization mass spectrometry (esi-ms) analysis of reverse transcription (rt)-pcr amplicons from human respiratory samples allows for broad pathogen identification approximately 8 h after collection. we investigated the performance characteristics of a high-throughput rt-pcr-coupled esi-ms assay for distinguishing biothreat (bt) agents from common bacterial, fungal, and viral respiratory pathogens in bronchoalveolar lavage (bal) fluid specimens from subjects with suspected respirator ... | 2013 | 23903543 |
| control of the escherichia coli sialoregulon by transcriptional repressor nanr. | nanr, one of >8,500 gntr superfamily helix-turn-helix transcriptional regulators, controls expression of the genes required for catabolism of sialic acids in escherichia coli. it is predicted to do the same in related bacteria harboring orthologs of nanr. the sialic acids are a family of over 40 naturally occurring nine-carbon keto-sugar acids found mainly in the animal lineage, which includes starfish to humans in the deuterostome lineage. sialic acids function in development, immunity, protein ... | 2013 | 23935044 |
| enhanced diagnostic yields of bacteremia and candidemia in blood specimens by pcr-electrospray ionization mass spectrometry. | a prospective study was performed to determine the value of direct molecular testing of whole blood for detecting the presence of culturable and unculturable bacteria and yeasts in patients with suspected bloodstream infections. a total of 464 adult and pediatric patients with positive blood cultures matched with 442 patients with negative blood cultures collected during the same period were recruited during a 10-month study. pcr amplification coupled with electrospray ionization mass spectromet ... | 2013 | 23966503 |
| identification of concurrent bacterial infection in adult patients with dengue. | we aim to construct a diagnostic model for bacterial coinfection in dengue patients (dengue dual infection score [ddis]); 2,065 adult dengue patients (mean age = 41.9 ± 17.2 years, 58.4% male, 83 patients with bacterial coinfection) seen at a university hospital from january of 2005 to february of 2010 were studied. the ddis was created by assigning one point to each of five risk factors for bacterial coinfection: pulse rate ≥ 90 beats/minute, total white cell count ≥ 6 × 10(9)/l, hematocrit < 4 ... | 2013 | 23980129 |
| heterogeneity among septic shock patients in a set of immunoregulatory markers. | immune activation is a regular feature of sepsis, but the incidence and nature of the ensuing inflammation-resolving and immunosuppressive component is less well understood. in this study, we compared immunoregulatory markers on blood leukocytes from patients with gram-negative or gram-positive sepsis or septic shock, and compared this to blood from patients with severe virosis or healthy controls. to this end, blood from 32 patients with sepsis, including ten cases with shock, and 12 patients w ... | 2013 | 23990136 |
| heterogeneity among septic shock patients in a set of immunoregulatory markers. | immune activation is a regular feature of sepsis, but the incidence and nature of the ensuing inflammation-resolving and immunosuppressive component is less well understood. in this study, we compared immunoregulatory markers on blood leukocytes from patients with gram-negative or gram-positive sepsis or septic shock, and compared this to blood from patients with severe virosis or healthy controls. to this end, blood from 32 patients with sepsis, including ten cases with shock, and 12 patients w ... | 2013 | 23990136 |
| the nad-dependent deacetylase sirtuin 2 is a suppressor of microglial activation and brain inflammation. | deleterious sustained inflammation mediated by activated microglia is common to most of neurologic disorders. here, we identified sirtuin 2 (sirt2), an abundant deacetylase in the brain, as a major inhibitor of microglia-mediated inflammation and neurotoxicity. sirt2-deficient mice (sirt2(-/-)) showed morphological changes in microglia and an increase in pro-inflammatory cytokines upon intracortical injection of lipopolysaccharide (lps). this response was associated with increased nitrotyrosinat ... | 2013 | 24013120 |
| solubilization of insoluble inorganic phosphate by burkholderia cepacia da23 isolated from cultivated soil. | a mineral phosphate solubilizing bacterium, burkholderia cepacia da23 has been isolated from cultivated soils. phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. when 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. mineral phosphate solubilization was directly related to the ph drop by the strain. analysis of the culture medium by high pressure liquid c ... | 2008 | 24031195 |
| microbiological and histopathological aspects of canine pyometra. | as pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the e. coli isolates identifying possible risks to human health. the microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the e. coli strains were identified using pcr method. this study al ... | 2008 | 24031249 |
| aerobic bacterial microflora of broad-snouted caiman (caiman latirostris) oral cavity and cloaca, originating from parque zoológico arruda câmara, paraíba, brazil. | the objective of this study was to isolate and identify the aerobic bacterial microflora from the oral cavity mucosa and cloaca's samples, collected from broad-snouted caiman (caiman latirostris), born and bred in captivity at parque zoológico arruda câmara, joão pessoa, paraíba, brazil. the most common bacteria were staphylococcus sp. (14.74%), corynebacterium sp. (13.68%), escherichia coli (13.68%) and shigella sp.(11.58%), and the less common were citrobacter sp. (1.05%), klebsiella pneumonia ... | 2009 | 24031343 |
| opportunistic microorganisms in patients undergoing antibiotic therapy for pulmonary tuberculosis. | antimicrobial therapy may cause changes in the resident oral microbiota, with the increase of opportunistic pathogens. the aim of this study was to compare the prevalence of candida, staphylococcus, pseudomonas and enterobacteriaceae in the oral cavity of fifty patients undergoing antibiotic therapy for pulmonary tuberculosis and systemically healthy controls. oral rinsing and subgingival samples were obtained, plated in sabouraud dextrose agar with chloramphenicol, mannitol agar and macconkey a ... | 2011 | 24031759 |
| high levels of extended-spectrum beta-lactamases in a major teaching hospital in ghana: the need for regular monitoring and evaluation of antibiotic resistance. | infections with bacteria producing extended-spectrum beta-lactamases (esbls) are increasing across africa. this study reports on esbl-producing enterobacteriaceae as significant causes of infections and antibiotic resistance at korle-bu teaching hospital in accra, ghana. of 300 isolates examined, 49.3% produced esbls. the prevalence of esbls was significantly high among isolates from neonates (28 of 43, 65.1%; relative risk = 1.62, 95% confidence interval = 1.33-2.13, p = 0.002) and adult patien ... | 2013 | 24043693 |
| evaluation of the βlacta test, a rapid test detecting resistance to third-generation cephalosporins in clinical strains of enterobacteriaceae. | for decades, third-generation cephalosporins (3gc) have been major drugs used to treat infections due to enterobacteriaceae; growing resistance to these antibiotics makes the rapid detection of such resistance important. the βlacta test is a chromogenic test developed for detecting 3gc-resistant isolates from cultures on solid media within 15 min. a multicenter prospective study conducted in 5 french and belgian hospitals evaluated the performance of this test on clinical isolates. based on anti ... | 2013 | 24068012 |
| genetic characterization of atypical citrobacter freundii. | the ability of a bacterial population to survive in different niches, as well as in stressful and rapidly changing environmental conditions, depends greatly on its genetic content. to survive such fluctuating conditions, bacteria have evolved different mechanisms to modulate phenotypic variations and related strategies to produce high levels of genetic diversity. laboratories working in microbiological diagnosis have shown that citrobacter freundii is very versatile in its colony morphology, as ... | 2013 | 24069274 |
| molecular basis of active copper resistance mechanisms in gram-negative bacteria. | copper is a metallic element that is crucial for cell metabolism; however, in extended concentrations, it is toxic for all living organisms. the dual nature of copper has forced organisms, including bacteria, to keep a tight hold on cellular copper content. this challenge has led to the evolution of complex mechanisms that on one hand enable them to deliver the essential element and on the other to protect cells against its toxicity. such mechanisms have been found in both eukaryotic and prokary ... | 2013 | 24072389 |
| pneumorachis associated with multiorgan infection due to citrobacter koseri. | pneumorachis rarely occurs after spreading from a contiguous site of infection or after a traumatic event. we describe an adult patient who developed sepsis and a renal abscess due to citrobacter koseri, and computed tomographic imaging identified gas within the entire spinal canal as well as an iliopsoas abscess. this patient recovered from pneumorachis caused by disseminated infection. | 2013 | 24075632 |
| curli biogenesis: order out of disorder. | many bacteria assemble extracellular amyloid fibers on their cell surface. secretion of proteins across membranes and the assembly of complex macromolecular structures must be highly coordinated to avoid the accumulation of potentially toxic intracellular protein aggregates. extracellular amyloid fiber assembly poses an even greater threat to cellular health due to the highly aggregative nature of amyloids and the inherent toxicity of amyloid assembly intermediates. therefore, temporal and spati ... | 2013 | 24080089 |
| curli biogenesis: order out of disorder. | many bacteria assemble extracellular amyloid fibers on their cell surface. secretion of proteins across membranes and the assembly of complex macromolecular structures must be highly coordinated to avoid the accumulation of potentially toxic intracellular protein aggregates. extracellular amyloid fiber assembly poses an even greater threat to cellular health due to the highly aggregative nature of amyloids and the inherent toxicity of amyloid assembly intermediates. therefore, temporal and spati ... | 2013 | 24080089 |
| a study on the ampc production amongst the urinary enterobacteriaceae isolates. | 2013 | 24086931 | |
| trends in human fecal carriage of extended-spectrum β-lactamases in the community: toward the globalization of ctx-m. | in the last 10 years, extended-spectrum β-lactamase-producing enterobacteria (esbl-e) have become one of the main challenges for antibiotic treatment of enterobacterial infections, largely because of the current ctx-m enzyme pandemic. however, most studies have focused on hospitalized patients, though today it appears that the community is strongly affected as well. we therefore decided to devote our investigation to trends in esbl-e fecal carriage rates and comprehensively reviewed data from st ... | 2013 | 24092853 |
| antimicrobial activity of ceftolozane-tazobactam tested against enterobacteriaceae and pseudomonas aeruginosa with various resistance patterns isolated in u.s. hospitals (2011-2012). | ceftolozane/tazobactam, a novel antimicrobial agent with activity against pseudomonas aeruginosa (including drug-resistant strains) and other common gram-negative pathogens (including most extended-spectrum-β-lactamase [esbl]-producing enterobacteriaceae strains), and comparator agents were susceptibility tested by a reference broth microdilution method against 7,071 enterobacteriaceae and 1,971 p. aeruginosa isolates. isolates were collected consecutively from patients in 32 medical centers acr ... | 2013 | 24100499 |
| seasonally variable intestinal metagenomes of the red palm weevil (rhynchophorus ferrugineus). | the intestinal microbes residing in the red palm weevil (rpw, rhynchophorus ferrugineus) larva consume tender interior fibrous tissues of date palm trunks. the understanding of such microbiota at molecular level provides vital clues for the biological control of this devastating pest. using pyrosequencing and shotgun strategy, we first study taxonomic profiles of the microbiota sampled at different months (march, july and november), and then confirm the impact of high-temperature stress on the m ... | 2013 | 24102776 |
| aetiological agents of ventilator-associated pneumonia and its resistance pattern - a threat for treatment. | ventilator-associated pneumonia (vap) is a common type of nosocomial pneumonia encountered in intensive care units. there are several aetiological agents which make treatment challenging. improper antibiotic treatment of ventilated patients may lead to the emergence of multidrug resistant (mdr) pathogens. | 2013 | 24133535 |
| bacterial aetiological agents of intra-amniotic infections and preterm birth in pregnant women. | infection-related preterm birth is a leading cause of infant mortality and morbidity; knowledge of bacterial populations invading the amniotic cavity and the routes of invasion is required to make progress in the prevention of preterm birth. significant advances have been made in understanding bacterial communities in the vagina, but much less studied are intra-uterine bacterial populations during pregnancy. a systematic review of data published on the intra-uterine microbiome was performed; mol ... | 2013 | 24137568 |
| nitrate and periplasmic nitrate reductases. | the nitrate anion is a simple, abundant and relatively stable species, yet plays a significant role in global cycling of nitrogen, global climate change, and human health. although it has been known for quite some time that nitrate is an important species environmentally, recent studies have identified potential medical applications. in this respect the nitrate anion remains an enigmatic species that promises to offer exciting science in years to come. many bacteria readily reduce nitrate to nit ... | 2014 | 24141308 |
| a small rna activates cfa synthase by isoform-specific mrna stabilization. | small rnas use a diversity of well-characterized mechanisms to repress mrnas, but how they activate gene expression at the mrna level remains not well understood. the predominant activation mechanism of hfq-associated small rnas has been translational control whereby base pairing with the target prevents the formation of an intrinsic inhibitory structure in the mrna and promotes translation initiation. here, we report a translation-independent mechanism whereby the small rna rydc selectively act ... | 2013 | 24141880 |
| the flavin reductase msue is a novel nitroreductase that can efficiently activate two promising next-generation prodrugs for gene-directed enzyme prodrug therapy. | bacterial nitroreductase enzymes that can efficiently catalyse the oxygen-independent reduction of prodrugs originally developed to target tumour hypoxia offer great potential for expanding the therapeutic range of these molecules to aerobic tumour regions, via the emerging cancer strategy of gene-directed enzyme prodrug therapy (gdept). two promising hypoxia prodrugs for gdept are the dinitrobenzamide mustard pr-104a, and the nitrochloromethylbenzindoline prodrug nitro-cbi-dei. we describe here ... | 2013 | 24202330 |
| orbital cellulitis following silicone-sponge scleral buckles. | acute or chronic infection of the scleral explant is rare. we report seven cases of scleral explant infections that caused orbital cellulitis. | 2013 | 24204118 |
| phylogenomic analysis identifies gene gains that define salmonella enterica subspecies i. | comparative methods for analyzing whole genome sequence (wgs) data enable us to assess the genetic information available for reconstructing the evolutionary history of pathogens. we used the comparative approach to determine diagnostic genes for salmonella enterica subspecies i. s. enterica subsp. i strains are known to infect warm-blooded organisms regularly while its close relatives tend to infect only cold-blooded organisms. we found 71 genes gained by the common ancestor of salmonella enteri ... | 2013 | 24204679 |
| extended spectrum ß-lactamase- and constitutively ampc-producing enterobacteriaceae on fresh produce and in the agricultural environment. | the attribution of fresh produce to the overall community-associated exposure of humans to esbl- or ampc-producing bacteria is currently unknown. to address this issue, the prevalence of esbl- and ampc-producing enterobacteriaceae on fresh produce produced in the netherlands was determined. seven vegetable types that are consumed raw were selected: blanched celery, bunched carrots, chicory, endive, iceberg lettuce, mushrooms, and radish. the vegetables were mostly obtained from supermarkets. to ... | 2014 | 24211774 |
| hepatic portal venous gas: comparison of two cases. | context. hepatic portal venous gas (hpvg) is a rare and sinister finding. its mortality is associated with the underlying causative condition. when secondary to bowel ischaemia, mortality rates exceed 50%. case report. two cases of hpvg are described. one case describes hpvg in association with gastric ischaemia, with complete resolution following conservative management. the second case describes hpvg in association with widespread intra-abdominal ischaemia, with resultant mortality. conclusion ... | 2013 | 24223320 |
| antimicrobial and herbal drug resistance in enteric bacteria isolated from faecal droppings of common house lizard/gecko (hemidactylus frenatus). | from 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (ifs,18) and hostels, guest houses, and dining rooms of different canteen/mess (hgm, 28), 326 bacterial isolates of enteric bacteria belonging to 17 genera and 34 species were detected. escherichia coli were the most frequently (39) isolated followed by citrobacter freundii (33), klebsiella pneumonia (27), salmonella indica (12), enterobacter gergoviae (12), and ent. agglomeran ... | 2013 | 24223595 |
| voided midstream urine culture and acute cystitis in premenopausal women. | the cause of acute uncomplicated cystitis is determined on the basis of cultures of voided midstream urine, but few data guide the interpretation of such results, especially when gram-positive bacteria grow. | 2013 | 24224622 |
| so depression is an inflammatory disease, but where does the inflammation come from? | we now know that depression is associated with a chronic, low-grade inflammatory response and activation of cell-mediated immunity, as well as activation of the compensatory anti-inflammatory reflex system. it is similarly accompanied by increased oxidative and nitrosative stress (o&ns), which contribute to neuroprogression in the disorder. the obvious question this poses is 'what is the source of this chronic low-grade inflammation?' | 2013 | 24228900 |
| the outcome of non-carbapenem-based empirical antibacterial therapy and vre colonisation in patients with hematological malignancies. | febrile neutropenia (fn) is generally a complication of cancer chemotherapy. | 2013 | 24235937 |
| medical and neurosurgical management of citrobacter koseri, a rare cause of neonatal meningitis. | 2014 | 24243285 | |
| comparative sequence, structure and redox analyses of klebsiella pneumoniae dsba show that anti-virulence target dsba enzymes fall into distinct classes. | bacterial dsba enzymes catalyze oxidative folding of virulence factors, and have been identified as targets for antivirulence drugs. however, dsba enzymes characterized to date exhibit a wide spectrum of redox properties and divergent structural features compared to the prototypical dsba enzyme of escherichia coli dsba (ecdsba). nonetheless, sequence analysis shows that dsbas are more highly conserved than their known substrate virulence factors, highlighting the potential to inhibit virulence a ... | 2013 | 24244651 |
| application of potential phosphate-solubilizing bacteria and organic acids on phosphate solubilization from phosphate rock in aerobic rice. | a study was conducted at universiti putra malaysia to determine the effect of phosphate-solubilizing bacteria (psb) and organic acids (oxalic & malic) on phosphate (p) solubilization from phosphate rock (pr) and growth of aerobic rice. four rates of each organic acid (0, 10, 20, and 30 mm), and psb strain (bacillus sp.) were applied to aerobic rice. total bacterial populations, amount of p solubilization, p uptake, soil ph, and root morphology were determined. the results of the study showed sig ... | 2013 | 24288473 |
| the phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life. | covering: up to 2013. although holo-acyl carrier protein synthase, acps, a phosphopantetheinyl transferase (pptase), was characterized in the 1960s, it was not until the publication of the landmark paper by lambalot et al. in 1996 that pptases garnered wide-spread attention being classified as a distinct enzyme superfamily. in the past two decades an increasing number of papers have been published on pptases ranging from identification, characterization, structure determination, mutagenesis, inh ... | 2014 | 24292120 |