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[etiology of keratoconjunctivitis in cattle. research of moraxella in n'dama bulls in senegal]. 19883194560
ovine keratoconjunctivitis experimentally induced by instillation of mycoplasma conjunctivae.five sheep, free from mycoplasma conjunctivae and ocular chlamydia infection, were experimentally inoculated with m. conjunctivae and five more sheep were exposed to the infection by contact. keratoconjunctivitis developed in all ten sheep. as in natural outbreaks of infectious keratoconjunctivitis (ikc), clinical signs were generally moderate and transient, and recurred in some sheep. m. conjunctivae was detected throughout the 53-day observation period. clinical diagnosis was confirmed by hist ...19883218062
[a discussion apropos g. p. kalina's article "species-forming involution of pathogenic bacteria as a biological law (exemplified by bacteria of the genus moraxella)"]. 19883218419
growth dependent enzymatic profiles of some gram-negative nonfermentative bacteria of clinical significance.a total of 734 strains of gram-negative nonfermentative bacteria (46 species and biochemically defined groups) of the genera pseudomonas, alcaligenes, bordetella, moraxella, acinetobacter, agrobacterium, and flavobacterium were investigated for their ability to hydrolyze 25 different chromogenic substrates. all tests were carried out in growth-stimulating media. results were read photometrically and evaluated automatically following a 24-h incubation. many of the 46 different species and biochem ...19883223128
[methods of detection and primary identification of bacteria of the genus moraxella in the environment]. 19883224839
beta-lactamase production in the upper respiratory tract flora in relation to antibiotic consumption: a study in children attending day nurseries.the occurrence of beta-lactamase production in haemophilus influenzae, branhamella catarrhalis and moraxella nonliquefaciens was compared in 191 healthy children attending day nurseries in 2 municipalities differing with regard to the prescription rate of beta-lactam antibiotics. a significantly higher frequency of beta-lactamase production was recorded in m. nonliquefaciens isolated in the municipality with the higher prescription rate. a corresponding difference was not recorded for h. influen ...19883261446
changes in aerobic microflora of skin and gills of mediterranean sardines (sardina pilchardus) during storage in ice.sardines from the adriatic sea were examined fresh and after 4 and 8 days of storage in ice. a total of 1500 strains isolated were identified from the gills and the surface of the fish. pseudomonadaceae, neisseriaceae, flavobacterium/cytophaga, enterobacteriaceae, coryneform bacteria and micrococcaceae were the most common bacteria in fresh fish. during storage the pseudomonads (mainly the non-fluorescent strains) increased and became the dominating microflora; the neisseriaceae (moraxella, psyc ...19883275304
[comparative study of various api galeries for the identification of gram negative bacteria].116 strains of gram negative bacteria were identified with the use of api atb 32 gn and rapid 20 e galeries, in order to evaluate their performance as compared with api 20 e or api ne galeries used as reference. the identification concur (bacterial genus and species) in approximately 80 per cent of the cases. there are only 2 major discrepancies with abt 32 gn galleries and only one with rapid 20 e. in other cases, the profile that is obtained only permits identification of the genus but without ...19883291654
infectious bovine keratoconjunctivitis: seasonal variation in cultural, biochemical and immunoreactive properties of moraxella bovis isolated from the eyes of cattle.the eyes of 20 young cattle were examined over an 18 month period in which 12 members of the group contracted infectious bovine keratoconjunctivitis (ibk). on each of 23 occasions cultural, biochemical and immunoreactive properties of up to 6 isolates of moraxella bovis derived from each eye were determined. relationships between the clinical response of eyes, phenotypic properties of m. bovis and annual variations in the level of solar ultraviolet radiation of 280 to 320 nm wavelength were exam ...19873300626
comparative efficacies of erythromycin-sulfisoxazole and cefaclor in acute otitis media: a double blind randomized trial.a prospective double blind trial compared the fixed combination of erythromycin-sulfisoxazole (e/s) with cefaclor in the treatment of acute otitis media. one hundred nineteen children in six centers across canada were studied. diagnostic tympanocentesis of 134 ears yielded 135 bacterial isolates: streptococcus pneumoniae (42%); haemophilus influenzae (21%); branhamella catarrhalis (10%); streptococcus pyogenes (5%); and other bacteria (22%). seventy-seven percent of strains of b. catarrhalis and ...19873302918
a comparison of two incubation temperatures for the isolation of gram-negative contaminants from raw materials and non-sterile pharmaceuticals.selective and non-selective broth enrichment techniques may be used in the isolation of microbial contaminants from pharmaceutical products. a non-selective method may give better recovery rates for damaged organisms. a trial was carried out to determine whether the recovery of gram-negative contaminants could be improved by using an incubation temperature of 30 degrees c for 48 h, rather than the more widely used 37 degrees c for 24 h. contaminants were isolated from 3.2% of samples incubated a ...19873305531
kingella kingae endocarditis: report of a case and review of the literature. 19873308781
epidemiology of microbial keratitis in southern california. a multivariate analysis.two hundred twenty-seven cases of microbial keratitis reported in nonreferral county practice were studied. the staphylococci, pseudomonas aeruginosa and streptococcus pneumoniae, were the major isolates. a multivariate statistical model was developed to evaluate possible predisposing and outcome determinants. several racial and age-related relationships were shown. the interaction of numerous local ocular and systemic factors played a fundamental role in causing disease. the authors found signi ...19873317183
endocarditis caused by kingella indologenes.the first known case of endocarditis caused by kingella indologenes is reported. a review of the literature reveals only seven cases of endocarditis caused by the other two species of the genus kingella (kingella kingae, six cases; kingella denitrificens, one case). kingella organisms appear to be sensitive to a wide variety of antimicrobial agents. the available data suggest that endocarditis caused by kingella species occurs rarely and is associated with a benign clinical course.19873326125
kingella kingae osteoarthritis and osteomyelitis in children.kingella kingae is still a poorly understood germ which is responsible for osteoarticular infections. we observed nine cases of osteoarthritis involving k. kingae in children. they included 4 cases of arthritis, 2 of osteomyelitis, and 3 of spondylodiscitis. the germ was cultured in all cases by direct surgical approach or by needle aspiration of the septic locus. evolution was always good after immobilization and nonspecific antibiotherapy. our nine clinical cases, along with the few cases alre ...19883335623
osteomyelitis due to kingella kingae infection.a case of osteomyelitis due to kingella kingae is presented to illustrate the insidious onset and indolent course typical of bone infections caused by this organism. the frequently negative result obtained with gram's stain and the fastidious nature of the organism makes diagnosis difficult. initial therapy with an aminopenicillin such as ampicillin in addition to antimicrobial drugs for staphylococcus aureus should be effective in resolving bone and joint infections in children.19883337967
cultural and chemical characterization of cdc groups eo-2, m-5, and m-6, moraxella (moraxella) species, oligella urethralis, acinetobacter species, and psychrobacter immobilis.we determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of cdc groups eo-2, m-5, and m-6, moraxella (moraxella) species, oligella urethralis, acinetobacter species, and psychrobacter immobilis. all organisms contained ubiquinone with eight isoprene units as the major isoprenolog, but distinct differences were observed in fatty acid composition. twenty-eight of the original collection of cdc group eo-2 strains were furthe ...19883356788
moraxella corneal ulcer. 19883373543
immunogenicity of a moraxella bovis bacterin containing attachment and cornea-degrading enzyme antigens.an adjuvanted moraxella bovis bacterin containing attachment antigens and cornea-degrading enzyme antigens protected cattle from infectious bovine keratoconjunctivitis (ibk) when experimentally challenged with homologous and heterologous challenge cultures of m. bovis. this bacterin also protected cattle against field exposure to m. bovis. transmission electron microscopy and fluorescein labeled anti-m. bovis pili antiserum showed pili on the m. bovis bacterin strain. scanning electron microscop ...19883376421
an attempt to induce clinical infectious keratoconjunctivitis in calves with naturally occurring moraxella bovis infection. 19883381465
purification and properties of formate dehydrogenase from moraxella sp. strain c-1.nad+-dependent formate dehydrogenase was screened in various bacterial strains. facultative methanol-utilizing bacteria isolated from soil samples, acclimated to a medium containing methanol and formate at ph 9.5, were classified as members of the genus moraxella. from a crude extract of moraxella sp. strain c-1, formate dehydrogenase was purified to homogeneity, as judged by disc gel electrophoresis. the enzyme has an isoelectric point of 3.9 and a molecular weight of approximately 98,000. the ...19883384805
identification of moraxella bovis by qualitative genetic transformation and nutritional assays.strains of moraxella bovis were identified definitively through the combined use of a qualitative genetic transformation assay and determination of the ability of the organism under examination to grow in a defined medium (medium mb). except for weak transformation by dna from strains of m. lacunata, m. nonliquefaciens, and m. (branhamella) ovis, dna samples from all other members of the genus moraxella failed to transform either of the two m. bovis auxotrophs used in this study.19883389822
prevalence of ocular microorganisms in hospitalized and stabled horses.microorganisms from normal eyes of hospitalized and stabled horses were identified, and the frequency of isolation was compared between the 2 groups. using standard techniques, swab specimens from both eyes of 22 hospitalized horses and both eyes of 18 stabled horses were cultured for aerobic bacteria and fungi. ninety-six aerobic bacteria and 57 fungi were isolated. the predominant bacterial isolates were gram-positive organisms, most of which belonged to the genera corynebacterium, bacillus, s ...19883400913
isolation of branhamella ovis from conjunctivae of angora goats. 19883401153
the occurrence of mycoplasma conjunctivae in the netherlands and its association with infectious keratoconjunctivitis in sheep and goats.the occurrence of mycoplasma conjunctivae in the netherlands is reported for the first time. m. conjunctivae was detected in all five sheep flocks examined with clinical signs of infectious keratoconjunctivitis (ikc) and in none of five sheep flocks without these clinical signs. these observations suggest a primary aetiological role of this organism in ikc in the netherlands. m. conjunctivae was also detected on a goat farm with clinical signs of ikc. experiences in culturing this organism are r ...19883413973
degradation of naphthalene-2,6- and naphthalene-1,6-disulfonic acid by a moraxella sp.a naphthalene-2,6-disulfonic acid (2,6nds)-degrading moraxella strain was isolated from an industrial sewage plant. this culture could also be adapted to naphthalene-1,6-disulfonic acid as growth substrate. regioselective 1,2-dioxygenation effected desulfonation and catabolism to 5-sulfosalicylic acid (5ss), which also could be used as the sole carbon source. 5ss-grown cells exhibited high gentisate 1,2-dioxygenase activity. neither 5ss- nor gentisate-grown cells oxidized 2,6nds; therefore, 2,6n ...19883415238
characterisation of the outer membrane protein antigens of british field isolates of moraxella bovis.outer membranes of a single isolate of moraxella bovis were prepared and their purity evaluated by a comparison of the iodinated proteins from whole cells and on outer membranes. the protein patterns of the outer membranes of 10 isolates were examined by sds-polyacrylamide gel electrophoresis, and the antigenic relationship between proteins of different isolates determined by immunoblotting, using antiserum produced against the outer membrane of the single isolate of m. bovis. the overall protei ...19873433652
recovery of moraxella urethralis from clinical material. 19873440461
[application of hierarchical clustering analysis to the recognition of bacterial whole-cell fatty acid patterns]. 19873448813
conjunctival lesions caused by moraxella bovis in gnotobiotic calves.hemolytic moraxella bovis was instilled into the conjunctival sac of gnotobiotic calves, and conjunctivae were sampled serially after infection. bilateral lesions developed in seven of eight infected calves. histologically, m. bovis was first seen within swollen epithelial cells near the lid margins and occasionally within superficial epithelium in other areas. conjunctival erosions and ulcers were seen in later stages. scanning electron microscopy showed m. bovis in pits on surfaces of epitheli ...19873455084
upper respiratory tract infections. ecological and therapeutic aspects of beta-lactamase production with special reference to branhamella catarrhalis.available data indicate that the most common beta-lactamase produced by branhamella catarrhalis is plasmid mediated. the same enzyme occurs in moraxella nonliquefaciens, a commensal in the upper respiratory tract. the ability to produce the enzyme, which is known as bro-1, can be transferred by conjugation from m. nonliquefaciens to b. catarrhalis. since the first beta-lactamase-producing strains of b. catarrhalis appeared in 1977, the frequency of beta-lactamase production has increased rapidly ...19863488190
plasmid-mediated beta-lactamase in branhamella catarrhalis.the plasmid-mediated beta-lactamase in branhamella catarrhalis (bro-1), also occurring in moraxella nonliquefaciens, differs from other known plasmid-mediated beta-lactamases in gram-negative bacteria regarding substrate profile and isoelectric point. b. catarrhalis strains previously reported to produce beta-lactamases deviating from bro-1 were tested, and the beta-lactamases did not differ significantly from bro-1 in substrate profile, isoelectric point or relative substrate affinity index (rs ...19863488198
beta-lactamase production in the upper respiratory tract flora.in order to determine the recovery rate of species of the genera haemophilus and moraxella (including subgenus branhamella) from the upper respiratory tract and the incidence of beta-lactamase production within these genera, cultures were made of nose and throat swab specimens and adenoid tissue in 50 children undergoing adenoidectomy. haemophilus influenzae was isolated from 92% of the children. all children harboured strains of haemophilus spp. and in 46%, at least one strain produced the tem- ...19863490974
disk diffusion susceptibility of branhamella catarrhalis and relationship of beta-lactam zone size to beta-lactamase production.we tested 231 isolates of branhamella catarrhalis for beta-lactamase production and drug susceptibility by the national committee for clinical laboratory standards disk diffusion method. the nitrocephin disk (cefinase) identified beta-lactamase in 98% of the enzyme-producing strains, and a zone diameter of inhibition of less than or equal to 29 mm for penicillin correctly predicted the presence of beta-lactamase in 99% of the isolates. no resistance to erythromycin, tetracycline, trimethoprim-su ...19863492178
activation of b chronic lymphocytic leukaemia cells by branhamella catarrhalis.cells from the blood of patients with chronic lymphocytic leukaemia were cultured in the presence of two polyclonal activators of human b cells, the bacteria branhamella catarrhalis (bc) and staphylococcus aureus cowan 1 (sac). although the magnitude of the responses varied, cells from seven of the eight patients studied were induced to proliferate in response to bc. in contrast, the response to sac was low or negligible in seven of the eight patients, and only one patient responded well to this ...19873493206
in vitro activity of bmy 28100, a new oral cephalosporin.bmy 28100, a new oral cephalosporin, demonstrated good in vitro activity against common gram-positive and gram-negative respiratory and urinary tract pathogens. its activity was shown by microdilution techniques to be generally higher than those of ampicillin, cephalothin and cephalexin, but comparable to those of cefaclor and, except for haemophilus spp. and branhamella spp., to amoxicillin/clavulanate.19873494601
amoxycillin/clavulanate in acute purulent exacerbations of chronic bronchitis.twenty patients, all admitted to hospital with acute purulent exacerbations of chronic bronchitis associated with haemophilus influenzae, streptococcus pneumoniae or beta-lactamase producing branhamella catarrhalis were treated twice daily for ten days with amoxycillin/clavulanate. ten patients were first given 1000 mg amoxycillin with 200 mg potassium clavulanate intravenously bd for three days, before crossing to the standard oral regimen of 1000 mg amoxycillin + 250 mg potassium clavulanate b ...19873494724
comparative antibacterial activity of the new oral cephalosporin bmy-28100.the in vitro activity of bmy-28100 was compared with that of four other oral cephalosporins against gram-positive cocci, branhamella catarrhalis and haemophilus influenzae. bmy-28100 showed 5-20 times better activity against staphylococci and streptococci. methicillin-resistant staphylococci and enterococci were resistant to the drug. branhamella catarrhalis and haemophilus influenzae strains were moderately susceptible. time-kill curve studies showed bmy-28100 to be equally as active as benzylp ...19873497804
detection of beta-lactamase activity among clinical isolates of branhamella catarrhalis with six different beta-lactamase assays.a total of 74 different clinical isolates of branhamella catarrhalis were examined for their ability to produce beta-lactamase by six different beta-lactamase assays. these included a conventional tube and disk test, in which the chromogenic cephalosporin nitrocefin was used as a substrate; a disk procedure, in which pyridinium-2-azo-p-dimethylanaline cephalosporin was used as a substrate; broth and disk acidometric methods; and a conventional tube iodometric assay. a total of 58 of the study is ...19873497942
infectious bovine keratoconjunctivitis: evidence for genetic modulation of resistance in purebred hereford cattle.a vaccination study was conducted in a herd of purebred hereford cattle representing 4 selection (genetic) lines. for each of 2 years, half of the cattle were vaccinated with a pilus-enriched moraxella bovis bacterin. cows were vaccinated before parturition, and calves were vaccinated at 2 to 3 months of age. none of the cattle was vaccinated for 1 year preceding and 1 year after the 2 years in which cattle were vaccinated. there was a significantly (p less than 0.05) lower percentage of infecti ...19863516033
critical evaluation of the automicrobic system gram-negative identification card for identification of glucose-nonfermenting gram-negative rods.during a 6-month study we critically evaluated the accuracy of the automicrobic system gram-negative identification card (vitek systems, inc., hazelwood, mo.) in identifying glucose-nonfermenting gram-negative bacilli by testing 419 selected isolates in parallel with a conventional reference method. of 356 isolates included in the automicrobic system profile, a total of 307 (86.2%) were correctly identified, 36 (10.1%) were not identified, and 13 (3.7%) were misidentified. fifty-eight of 63 (92% ...19863517050
etiologic significance of bacterial isolates from rams with palpable epididymitis.identified and partly identified bacterial isolates were obtained from 48 rams of various breeds that had unilateral or bilateral epididymitis. most of the animals were approximately 1 year of age; a few were older. brucella ovis, actinobacillus spp, corynebacterium spp, haemophilus spp, acinetobacter spp, escherichia coli, moraxella spp, staphylococcus spp, pasteurella spp, streptococcus spp, and chlamydia psittaci were isolated. a vaccine strain of b ovis, isolated species of bacteria, and mix ...19863521407
[sensitivity of kingella kingae to antibiotics].kingella kingae is a small gram negative rod of the neisseriaceae family, formerly called moraxella kingae. this microorganism is found occasionally in the oral cavity and is capable of causing infections. we report three cases of septic arthritis in children due to k. kingae. in vitro susceptibility of the recovered strains was tested using determination of mics in agar. the strains were susceptible to penicillin, ampicillin, ticarcillin, cephalothin, cefotaxime, gentamicin, chloramphenicol, te ...19863534752
comparison of rapid nft system and conventional methods for identification of nonsaccharolytic gram-negative bacteria.this study examined the rapid nft system (analytab products, plainview, n.y.) to determine its ability to accurately identify 229 clinical isolates of mostly nonsaccharolytic gram-negative rods. identifications were classified by the following scheme: correct (corresponding to excellent, very good, good, or acceptable identification as listed in the code book); low discrimination (correct identification among a range of listed possibilities, with additional tests necessary for accurate identific ...19863536999
[bacteria of the genus moraxella. their systematics, differential diagnosis and the present status of the problem]. 19863541469
comparison of the heat resistance of bacterial lipases and proteases and the effect on ultra-high temperature milk quality.thirty-four of 49 isolates of psychrotrophic bacteria produced extracellular lipase or protease when grown in rehydrated nonfat dry milk. the cell-free crude preparation from 50% of these had either heat-resistant lipase or protease; in 30% both enzymes were heat resistant. eight isolates were selected for further evaluation of effect on ultra-high temperature processed milk. free fatty acids and free amino groups of milk precultured with the bacteria increased at different rates depending on th ...19863543080
[bacteria of the genus moraxella. their ecology]. 19873554854
ormetoprim-sulfadimethoxine in cattle: pharmacokinetics, bioavailability, distribution to the tears, and in vitro activity against moraxella bovis.the pharmacokinetics, bioavailability, and distribution to the tears of ormetoprim (omp; 5.5 mg/kg of body weight) and sulfadimethoxine (sdm; 27.5 mg/kg of body weight) were determined following iv or oral administration to 6 holstein steers. after iv administration, the disposition kinetics of both drugs were best described by a 2-compartment open model. sulfadimethoxine had a moderately rapid distribution phase, followed by a slower elimination phase, with a mean half-life (t 1/2) of 7.91 hour ...19873565896
role of dissolution rate and solubility in biodegradation of aromatic compounds.strains of moraxella sp., pseudomonas sp., and flavobacterium sp. able to grow on biphenyl were isolated from sewage. the bacteria produced 2.3 to 4.5 g of protein per mol of biphenyl carbon, and similar protein yields were obtained when the isolates were grown on succinate. mineralization of biphenyl was exponential during the phase of exponential growth of moraxella sp. and pseudomonas sp. in biphenyl-supplemented media, flavobacterium sp. had one exponential phase of growth apparently at the ...19873566268
taxonomy of pasteurella anatipestifer. 1. dna base composition and dna-dna hybridization analysis.dna was isolated from 15 strains of pasteurella anatipestifer and from one strain each of moraxella nonliquefaciens, m. bovis, pasteurella multocida, p. haemolytica, p. gallinarum, p. pneumotropica, and p. ureae. the guanine-plus-cytosine contents of p. anatipestifer ranged from 32 to 35 mole %, whereas those of moraxella and pasteurella spp. were much higher, ranging from 40 to 45 mole %. dna-dna hybridization analysis revealed that homology of nine p. anatipestifer strains to strains atcc 1184 ...19873579792
taxonomy of pasteurella anatipestifer. 2. cellular fatty-acid profile by gas chromatography.methylated cellular fatty acids of representative strains of pasteurella spp., moraxella spp., and p. anatipestifer were subjected to gas chromatography in an attempt to further support the independence of p. anatipestifer from both pasteurella and moraxella. all pasteurella spp. and moraxella spp. revealed group characteristics specific for each genus that could be easily differentiated from the unique profile of p. anatipestifer. all p. anatipestifer strains tested showed similar fatty-acid pr ...19873579793
kingella kingae septicaemia. 19873591214
pneumonia associated with branhamella catarrhalis in infants. 19873615071
bacterial tracheitis caused by branhamella catarrhalis. 19873615072
pathogenesis of corneal lesions caused by moraxella bovis in gnotobiotic calves.moraxella bovis was instilled into the conjunctival sac of gnotobiotic calves and corneas were sampled serially after infection. lesions developed in seven of eight infected calves, but were absent in a noninfected control calf. histologically, m. bovis was first seen in foci of swollen epithelium and within basal epithelial cells adjacent to ulcers. corneal ulcers were severe in later stages of infection; fibrin deposits, neutrophils, and bacteria were present in the stromas. examination of ear ...19873617395
isolation of a moraxella sp from horses with conjunctivitis. 19873619796
a note on hydrolysis of tributyrin by branhamella and neisseria.sixty-three strains of branhamella and neisseria were tested by two methods for their ability to hydrolyse glycerol tributyrate. after the conventional plate test, gas liquid chromatographical (glc) analysis of the agar medium was carried out to detect the hydrolysis product, butyric acid, and other volatile fatty acids. all strains of branhamella catarrhalis, neisseria caviae, n. cuniculi and n. ovis but no other neisseria spp. gave positive results with the conventional test. with glc, however ...19873624107
effect of inoculum size on results of macrotube broth dilution susceptibility tests with branhamella catarrhalis.the effect of inoculum size on the results of macrotube broth dilution mics was assessed with 15 strains of branhamella catarrhalis versus 22 antimicrobial agents. the study isolates included five beta-lactamase-negative strains, five strains that produced beta-lactamase and for which ampicillin mics were high, and five beta-lactamase-producing strains for which ampicillin mics were low. an inoculum effect was observed only with strains in the last group when tested versus the following antimicr ...19873624453
combined genetic transformation and nutritional assay for identification of moraxella nonliquefaciens.a combined genetic transformation and nutritional assay is described that permits definitive identification of clinically isolated strains of moraxella nonliquefaciens. crude dna preparations of strains of various moraxella species were used to transform nutritional mutants of a stably competent strain of m. nonliquefaciens for ability to grow on a defined medium (mn-b). dna samples from 24 independently isolated strains of m. nonliquefaciens all resulted in massive (4+) transformation of each o ...19873654942
an outbreak of moraxella (pasteurella) anatipestifer infection in ducklings in japan. 19873682537
cloxacillin and ibk. 19873686797
microbial flora of the eyes of cattle.the bacteria and mycoplasma occurring in the eyes of normal healthy calves were monitored in three groups of animals from purchase at about one week old to slaughter at about 15 months old. non-haemolytic moraxella bovis, branhamella catarrhalis and mycoplasma bovoculi were all isolated regularly, though their seasonal occurrence varied. the significance of these findings with respect to infectious bovine keratoconjunctivitis is discussed.19863716161
distribution of kanamycin in ocular tissues of calves.the ocular distribution of kanamycin following intramuscular, bulbar subconjunctival injection, or after constant rate intravenous infusion to calves was studied. steady-state plasma concentrations of kanamycin were achieved in either normal calves, or in those experimentally infected with moraxella bovis, and the concentrations of kanamycin in the serum, aqueous humor, vitreous body, tears, and the ocular tissues were measured. kanamycin was not detected in the retina, lens, vitreous body, or t ...19863723661
mycoplasma bovoculi infection increases ocular colonization by moraxella ovis in calves.to determine whether infection with mycoplasma bovoculi increases ocular colonization of cattle eyes with moraxella bovis and other bacteria, colonization of ocular gram-negative bacteria were measured in eyes of cattle infected with mycoplasma bovoculi. strains of moraxella ovis were chosen because these are among the most commonly isolated species of gram-negative bacteria from cattle eyes. five strains of m ovis were characterized biochemically and by pilus structure, permitting the recogniti ...19863729120
moraxella septic arthritis of the knee joint: a case report.moraxella osloensis pyarthrosis of the knee joint is an unusual infection. the diagnosis can be quite difficult since the organism is fastidious in its growth characteristics. this case report presents an adult with m. osloensis sepsis of the knee, which could only be diagnosed by arthroscopic synovial biopsy and culture. moreover, arthroscopy played a critical role in the definitive therapy.19863730070
phenotypic and genetic relationships of so-called moraxella (pasteurella) anatipestifer to the flavobacterium/cytophaga group.so-called moraxella (or pasteurella) anatipestifer and members of the flavobacterium/cytophaga group exhibit remarkable common features: lack of flagellation, low guanine + cytosine content of the chromosomal dna, production of menaquinones and branched-chain fatty acids, absence of carbohydrate fermentation, and similar patterns of hydrolytic enzymes. using the renaturation method of dna:dna hybridization two urease-negative european isolates and the urease-positive type strain (which was isola ...19863739211
outer membrane protein antigens of moraxella bovis.outer membranes were isolated from bovine isolates and type strains of moraxella bovis, m phenylpyruvica, m lacunata, and m ovis by sodium n lauroyl sarcosinate extraction and differential centrifugation. analysis of outer membranes from these organisms by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis revealed that all m bovis isolates shared a common polypeptide pattern that was readily distinguishable from other moraxella spp. nine major outer membrane protein bands were identified ...19863740610
infectious bovine keratoconjunctivitis: comparison of infection, signs of disease and weight gain in vaccinated versus nonvaccinated purebred hereford heifer calves.to determine whether the carrier state of moraxella bovis could be prevented under natural conditions of exposure by vaccination, purebred hereford calves were vaccinated twice; once in june 1982, and again in september 1982. eyes of calves were examined for signs of infectious bovine keratoconjunctivitis (ibk) and cultured for m. bovis in june 1982, september 1982, january 1983 and june 1983. when calves were compared on the basis of selection lines (four genetic groups), there were significant ...19863756679
endocarditis with moraxella-like m-6 after cardiac catheterization.a patient developed bacteremia with cdc group m-6, a moraxella-like bacterium, after a complicated heart catheterization. he was treated with tobramycin and ampicillin. the aortic valve was later replaced and did not show any signs of infection. the slow growth of m-6 can delay diagnosis and give misleading antibiotic susceptibility results. penicillin is not always active against this organism.19863760147
infections caused by kingella kingae: report of four cases and review.kingella kingae, formerly known as moraxella kingae, is a fastidious, non-motile, coccobacillary, fermentative gram-negative rod that has been chiefly associated with two types of infections in man: bone and joint infections, and endocarditis. we describe four patients with k. kingae infections, one with septicaemia, two with endocarditis, and one with osteoarthritis. the current literature on infections with k. kingae is reviewed.19863760596
defined medium for moraxella (branhamella) catarrhalis.a defined medium for growth of 24 strains of moraxella (branhamella) catarrhalis was devised. this medium (medium b4) contains sodium lactate as a partial carbon source, proline as both a partial carbon source and a partial nitrogen source, aspartate as a partial nitrogen source, and the growth factors arginine, glycine, and methionine. either aspartate, glutamate, or proline could serve as sole nitrogen source, but growth occurred at a significantly better rate if proline was present together w ...19863767360
moraxella catarrhalis: antibiotic susceptibility with special reference to trimethoprim. 19863771428
use of subcutaneously implanted chambers in calves for in vivo study of isolates of moraxella bovis.selected cultures of moraxella bovis were studied in calves, using chambers fabricated from a semipermeable membrane supported and protected by perforated plastic golf balls. plain balls, semipermeable membrane-covered balls, and balls that contained bags fabricated from semipermeable membranes were surgically implanted subcutaneously in calves at a site approximately 25 cm ventral to the paralumbar fossa and cranial to the prefemoral lymph node. after calves recovered from surgery, chambers wer ...19863777650
defined medium for moraxella bovis.a defined medium (medium mb) for moraxella bovis was formulated. nineteen strains grew well on medium mb. one strain was auxotrophic for asparagine, and another was auxotrophic for methionine. strains of m. equi and m. lacunata also grew on medium mb. all strains had an absolute requirement for thiamine and were stimulated by or actually required the other growth factors in the medium.19863777935
use of conductance methods to predict bacterial counts in fish.conductance methods to measure bacterial growth are more rapid than conventional methods for assessing the load of spoilage bacteria in fish. with the correct choice of medium, an estimate of the count can be obtained within 24 h which shows a very good correlation with the conventional methods. moreover, the conductance changes correlate better with counts of those organisms thought to be responsible for spoilage. the malthus conductance instrument provides an automated system capable of the si ...19863781938
[characteristics of various species of bacteria of the genus moraxella isolated from the urogenital system]. 19863786808
identification of bovine carriers of moraxella bovis by comparative cultural examinations of ocular and nasal secretions.the carrier state of moraxella bovis was investigated, using bacteriologic examinations of ocular and nasal secretions from cattle under experimental and natural conditions of exposure and management. moraxella bovis was isolated throughout the year from the ocular and nasal secretions of cattle naturally affected with infectious bovine keratoconjunctivitis. there was also 1 case of nasal transmission of m bovis without isolation of m bovis from ocular secretions and 1 case of m bovis isolation ...19863789493
[osteoarthritis and osteomyelitis due to kingella kingae in children. apropos of 5 cases and review of the literature].bone infection caused by kingella kingae (kk) is rare since there are only 15 published cases. we report 5 additional cases with isolation of kk from fluid or bone aspiration. kk was sensitive to the most common antibiotics. the outcome was favorable in every case.19863808839
investigation of the effect of moraxella phenylpyruvica on the genital tract of the sow. 19863811184
a numerical taxonomic study of actinobacillus, pasteurella and yersinia.a numerical taxonomic study of strains of actinobacillus, pasteurella and yersinia, with some allied bacteria, showed 23 reasonably distinct groups. these fell into three major areas. area a contained species of actinobacillus and pasteurella: a. suis, a. equuli, a. lignieresii, p. haemolytica biovar a, p. haemolytica biovar t, p. multocida, a. actinomycetemcomitans, 'p. bettii', 'a. seminis', p. ureae and p. aerogenes. also included in a was a composite group of pasteurella pneumotropica and p. ...19853840837
adherence of moraxella bovis to tissue culture cells. 19853842146
characterization of the plasmid-mediated beta-lactamase in branhamella catarrhalis, with special reference to substrate affinity.the plasmid-mediated branhamella catarrhalis beta-lactamase bro-1, also found in moraxella nonliquefaciens, was characterized as regards substrate profile, isoelectric point and relative substrate affinity index (rsai) to various substrates and compared in these aspects with the tem-1 enzyme of haemophilus influenzae. as measured by a biological assay and with high performance liquid chromatography (hplc), bro-1 was found to hydrolyse carbenicillin, mecillinam, methicillin and cefaclor with a hi ...19853872294
identification of gram-negative bacilli using the autobac idx.the autobac idx is a new system for the rapid identification of clinically significant members of the enterobacteriaceae and aeromonas, acinetobacter, alcaligenes, flavobacterium, moraxella, and pseudomonas species. the use of 18 differentially inhibitory compounds such as dyes and antibiotics along with a computerized algorithm based on a multivariate analysis provides the basis for the identification of 30 different groups of gram-negative bacilli. required preliminary tests include observatio ...19853881212
the use of immuno-peroxidase techniques for the identification of moraxella bovis in tissue sections. 19853888344
evaluation of api 20 ne in routine diagnostics of nonfermenting gram-negative rod-shaped bacteria.292 strains of non-fermenting gram-negative rod-shaped bacteria were determined by standard methods and tested in a new commercial microidentification-system, the api 20 ne (api biomérieux). a total of 282 (= 96,6%) strains were identified conformably; 130 (= 46,1%) after 24 h and 77 (= 27,3%) within 48 h. in 75 (= 26,6%) cases different conventional additional tests for determination to species level were necessary. the reasons for divergent results of 10 strains are discussed. the new microide ...19853890425
b-scan ultrasonography for the detection of space-occupying ocular masses.a noninvasive technique, b-scan ultrasonography, was used to obtain detailed cross-sectional images of ocular and orbital structure and form. when a low-frequency probe (5 mhz) was used, tissue penetration was excellent; however, axial resolution was limited. the technique was used in a study of 2 cats and 1 dog. the use of low-frequency b-scan ultrasound should aid in the diagnostic examinations of space-occupying masses in the posterior portion of the ocular bulb and the orbit.19853894305
moraxella lacunata isolated from epidemic conjunctivitis among teen-aged females.an epidemic follicular conjunctivitis affecting teen-aged females has been studied. moraxella lacunata was isolated from 11% of the patients, a frequency far above the rate found in other types of chronic conjunctivitis. the condition is suggested to be the chronic follicular conjunctivitis (axenfeld) due to moraxella lacunata.19853901656
quantitative and qualitative aspects of bacterial flora of karachi coastal water shrimp (penaeus merguiensis and metapenaeus monoceros).bacterial counts were made over a period of two years of two important commercial shrimp varieties of karachi coastal waters. bacteria were also isolated and identified. total number of bacteria were found to be remarkably equal at 37 degrees, 30 degrees and 25 degrees c. aerobic plate count of penaeus merguiensis at 37 degrees c ranged from 1.2 x 10(5) to 6.0 x 10(7) cfu/g (median 1.8 x 10(6) cfu/g), and were predominantly vibrio, micrococcus, pseudomonas, staphylococcus, bacillus, and flavobac ...19853913252
evaluation of the rim system and gono gen test for identification of neisseria gonorrhoeae from clinical specimens.rim-n (austin biological laboratories) and gono gen (micro media systems) were evaluated for accuracy and compared with conventional carbohydrate degradation tests for identification of neisseria gonorrhoeae. a total of 127 fresh clinical isolates of n. gonorrhoeae were tested; 118 (93%) were identified by rim-n, and 100 (79%) yielded positive reactions with gono gen. seventy nongonococcal isolates including other neisseria species, branhamella catarrhalis, kingella kingae, and moraxella species ...19853919991
beta-lactamase susceptibility and comparative activity of sch 34343 and other beta-lactams for non-fermenters, neisseria spp. and beta-lactamase-positive enterobacteriaceae.by means of a micro-dilution technique (10(5) cfu/cup) 30 strains of pseudomonas spp. were found resistant to sch 34343. the susceptibility of acinetobacter spp. (20 strains) was greatest for imipenem, followed by sch 34343 (mic approximately 0.7 mg/l), ceftazidime and ceftriaxone. ten moraxella strains were very susceptible to sch 34343, imipenem and ceftazidime. for 35 strains of neisseria gonorrhoeae (ten beta-lactamase-positive) and ten strains of n. meningitidis we noted no resistance to sc ...19853928576
changes in titres of moraxella bovis-specific igg in growing cattle. 19863947934
scanning electron microscopy of bovine corneas irradiated with sun lamps and challenge exposed with moraxella bovis.effects of uv radiation and irradiation followed by challenge exposure with moraxella bovis on the corneal epithelium were studied in 6 calves by scanning electron microscopy. after uv irradiation, the number of dark cells comprising the surface epithelium increased. many epithelial cells were in various states of degeneration and were characterized initially by large round nuclei, whereas sloughing and peeling were characteristic of the last degenerative stage. all m bovis-infected irradiated e ...19863954223
incidence of moraxella conjunctival infection.thirty-three cases of moraxella conjunctival infection, many incorrectly diagnosed as chlamydia, epidemic keratoconjunctivitis, and herpes simplex infections, were confirmed by bacterial cultures and giemsa staining during a one-year period. although moraxella conjunctivitis is associated with adolescent girls, 13 of our 27 female patients with moraxella infection (48%) were 20 years or older. although corneal involvement, preauricular adenopathy, and ocular nodules are not classically associate ...19863963104
infectious bovine keratoconjunctivitis: contact transmission.the transmission of moraxella bovis was studied in calves in the absence of the face fly (musca autumnalis) or environmental conditions that might insult the eye. thirty calves were placed in 10 groups of 1 experimentally infected calf and 2 contact calves each. over 40 days, only 1 eye in 1 contact calf developed clinical infectious bovine keratoconjunctivitis. the organism was recovered in only 8 of 20 contact calves, whereas infection and disease occurred in all experimentally infected calves ...19863963562
plasmid profiles of moraxella bovis isolates.two-hundred isolates of moraxella bovis were selected at random and examined for the presence of plasmid dna by a rapid alkaline-detergent lysis method. all isolates contained from 1 to 6 plasmids, with varying agarose-gel electrophoretic migration patterns. most (80%) isolates carried 2 to 4 plasmids, which ranged in molecular weight from 2.6 to 80 megadaltons. seemingly, plasmid profiles can be used as a simple, reliable epizootiologic tool to establish a strain identification scheme for m bov ...19863963603
branhamella catarrhalis bacteraemia and immunosuppression--part of a larger problem?attention is drawn to the striking association between bacteraemia due to branhamella catarrhalis and immunosuppression and evidence is cited suggesting a special relationship between this and other neisseria of intermediate virulence and systemic disease in the immunologically compromised.19853967473
the demethylation of guaiacol by a new bacterial cytochrome p-450.spectroscopic studies were carried with a cytochrome p-450 in moraxella sp., strain gu2, that could grow on guaiacol or 2-ethoxyphenol as the sole source of carbon and energy. the dissociation constant of the guaiacol-cytochrome complex was estimated to 0.15 microm, as determined in vivo or using the cell soluble extract. cytochrome p-450 could also bind 2-ethoxyphenol, 2-propoxyphenol, and 2-butoxyphenol, and the dissociation constants have been determined in each case. metyrapone depressed the ...19853970527
moraxella nonliquefaciens endophthalmitis after trabeculectomy.endophthalmitis caused by moraxella nonliquefaciens developed five years after trabeculectomy in a 67-year-old man and 15 months after trabeculectomy in a 62-year-old women. symptoms in both cases included pain, inflammation, and decreased visual acuity. aggressive treatment with antibiotics and corticosteroids led to resolution within a few days.19853976811
[sepsis caused by moraxella nonliquefaciens]. 19853981909
moraxella bovis hemagglutinins: effect of carbohydrates, heating and erythrocytes.several properties of the adhesins of eight isolates of moraxella bovis recovered from cattle suffering from infectious keratoconjunctivitis, were studied. adhesions were detected through autoagglutination in saline and hemagglutination. autoagglutinating strains agglutinated red blood cells of the chicken, rabbit, sheep and swine, but not those of the guinea pig. the adhesins were not inhibited by d-mannose or d-galactose and resisted heating at 100 degrees c for 15 minutes. magnesium chloride ...19853986674
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