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first report of severe acute otitis media caused by campylobacter rectus and review of the literature.campylobacter rectus is a member of the human oral flora and is associated with periodontal disease. we report the first case of severe acute otitis media (aom) due to c. rectus in a previous healthy 15-year-old boy, which was confirmed by 16s ribosomal rna gene sequencing. c. rectus is a possible causative pathogen of aom.201627363873
clinical, radiographic and microbiological evaluation of high level laser therapy, a new photodynamic therapy protocol, in peri-implantitis treatment; a pilot experience.aim. endosseous implants are widely used to replace missing teeth but mucositis and peri-implantitis are the most frequent long-term complications related with dental implants. removing all bacterial deposits on contaminated implant surface is very difficult due to implant surface morphology. the aim of this study was to evaluate the bactericidal potential of photodynamic therapy by using a new high level laser irradiation protocol associated with hydrogen peroxide in peri-implantitis. materials ...201627379251
frequency of periodontal pathogens and helicobacter pylori in the mouths and stomachs of obese individuals submitted to bariatric surgery: a cross-sectional study.this cross-sectional study compared the frequency of oral periodontopathogens and h. pylori in the mouths and stomachs of obese individuals with or without periodontitis submitted to bariatric surgery.201627383704
the current weight of evidence of the microbiologic profile associated with peri-implantitis: a systematic review.there is currently no consensus regarding microorganisms that may be considered true peri-implant pathogens. therefore, the aim of this systematic review is to determine the weight of evidence for microorganisms related to peri-implantitis based on results of association studies.201627420109
the antimicrobial photodynamic therapy in the treatment of peri-implantitis.introduction. the aim of this study is to demonstrate the effectiveness of addition of the antimicrobial photodynamic therapy to the conventional approach in the treatment of peri-implantitis. materials and methods. forty patients were randomly assigned to test or control groups. patients were assessed at baseline and at six (t1), twelve (t2), and twenty-four (t3) weeks recording plaque index (pli), probing pocket depth (ppd), and bleeding on probing (bop); control group received conventional pe ...201627429618
smoking, pregnancy and the subgingival microbiome.the periodontal microbiome is known to be altered during pregnancy as well as by smoking. however, despite the fact that 2.1 million women in the united states smoke during their pregnancy, the potentially synergistic effects of smoking and pregnancy on the subgingival microbiome have never been studied. subgingival plaque was collected from 44 systemically and periodontally healthy non-pregnant nonsmokers (control), non-pregnant smokers, pregnant nonsmokers and pregnant smokers and sequenced us ...201627461975
evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study.the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ...201627469559
bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas.the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ...201627469562
oral behavior, dental, periodontal and microbiological findings in patients undergoing hemodialysis and after kidney transplantation.aim of this single center cross-sectional study was to investigate oral behavior, dental, periodontal and microbiological findings in patients undergoing hemodialysis (hd) and after kidney transplantation (kt).201627531498
longitudinal study on clinical and microbial analysis of periodontal status in pregnancy.this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ...201627556678
the role of nutrition in periodontal health: an update.periodontal health is influenced by a number of factors such as oral hygiene, genetic and epigenetic factors, systemic health, and nutrition. many studies have observed that a balanced diet has an essential role in maintaining periodontal health. additionally, the influences of nutritional supplements and dietary components have been known to affect healing after periodontal surgery. studies have attempted to find a correlation between tooth loss, periodontal health, and nutrition. moreover, bon ...201627589794
evaluation of moxifloxacin-hydroxyapatite composite graft in the regeneration of intrabony defects: a clinical, radiographic, and microbiological study.the formation of new connective periodontal attachment is contingent upon the elimination or marked reduction of pathogens at the treated periodontal site. an anti-microbial agent, i.e. moxifloxacin has been incorporated into the bone graft to control infection and facilitate healing during and after periodontal therapy.201627630501
effect of non-surgical periodontal treatment on transferrin serum levels in patients with chronic periodontitis.background. transferrin is a negative acute phase protein, which decreases during inflammation and infection. the aim of the present investigation was to evaluate changes in the transferrin serum levels subsequent to non-surgical treatment of chronic periodontal disease. methods. twenty patients with chronic periodontitis and 20 systemically healthy subjects without periodontal disease, who had referred to tabriz faculty of dentistry, were selected. transferrin serum levels and clinical periodon ...201627651883
thoracic empyema caused by campylobacter rectus.we report a case of thoracic empyema caused by campylobacter rectus, an organism considered as a periodontal pathogen but rarely recovered from extraoral specimens. the patient fully recovered through drainage of purulent pleural fluid and administration of antibiotics. the present case illustrates that c. rectus can be a cause of not only periodontal disease but also pulmonary infection.201727681234
does obesity influence the subgingival microbiota composition in periodontal health and disease?to evaluate whether obesity affects the subgingival microbial composition of patients with periodontal health or chronic periodontitis (cp).201627717180
salivary periodontopathic bacteria in children and adolescents with down syndrome.to assess and compare salivary periodontopathic bacteria between groups of down syndrome and non-down syndrome children and adolescents.201627727287
associations between periodontal microbiota and death rates.it is conceived that specific combinations of periodontal bacteria are associated with risk for the various forms of periodontitis. we hypothesized that such specificity is also related to human cause-specific death rates. we tested this hypothesis in a representative sample of the us population followed for a mean duration of 11 years and found that two specific patterns of 21 serum antibodies against periodontal bacteria were significantly associated with increased all-cause and/or diabetes-re ...201627748442
comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample?the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control).201727786617
the relationship between the immune system and oral manifestations of inflammatory bowel disease: a review.inflammatory bowel diseases (ibds) are chronic, relapsing inflammatory diseases characterized by exacerbations and remissions of the gastrointestinal tract, clinically manifested as crohn's disease and ulcerative colitis. the etiology of ibds is considered to be multi factorial, comprising environmental, immune, microbial and genetic factors. clinical signs may include abdominal pain, frequent bloody diarrheas, mucorrhea, vomiting, fever, fatigue or weight loss. changes in the oral cavity often ...201627833449
sensitivity and specificity of subgingival bacteria in predicting preterm birth- a pilot cohort study.preterm birth (ptb) increases the risk of adverse outcomes for new born infants. subgingival bacteria are implicated in causing ptb. the aim of the present study was to assess the accuracy of some subgingival gram positive and gram negative bacteria detected by routine lab procedures in predicting ptb.201627833518
microbial profiles and detection techniques in peri-implant diseases: a systematic review.to describe the microbial profiles of peri-implant diseases and the main detection methods.201627833735
peripheral blood leukocytes interleukin-1 beta (il-1β) cytokine hyper-reactivity in chronic periodontitis.background levels of pro-inflammatory cytokine (il-1β) released by peripheral blood leukocyte medium (pblm), isolated from chronic periodontitis patients (p) before therapy and matched to controls, were determined in the presence or absence of non-opsonized escherichia coli and staphylococcus aureus. material and methods in this investigation, 26 patients with untreated, severe, generalized, chronic periodontitis and 26 healthy subjects (h) were enrolled. periodontal status was assessed by measu ...201627847385
mefit: merging and filtering tool for illumina paired-end reads for 16s rrna amplicon sequencing.recent advances in next-generation sequencing have revolutionized genomic research. 16s rrna amplicon sequencing using paired-end sequencing on the miseq platform from illumina, inc., is being used to characterize the composition and dynamics of extremely complex/diverse microbial communities. for this analysis on the illumina platform, merging and quality filtering of paired-end reads are essential first steps in data analysis to ensure the accuracy and reliability of downstream analysis.201627905885
quantitative analysis of periodontal pathogens using real-time polymerase chain reaction (pcr).the quantitative polymerase chain reaction (qpcr) is a variant of pcr aimed to detect and quantify a targeted dna molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated pcr product. this chapter presents the detailed procedures for quantification of different periodontal pathogens (porphyromonas gingivalis, aggregatibacter actinomycetemcomitans ...201727924595
comparative metagenomics reveals taxonomically idiosyncratic yet functionally congruent communities in periodontitis.the phylogenetic characteristics of microbial communities associated with periodontitis have been well studied, however, little is known about the functional endowments of this ecosystem. the present study examined 73 microbial assemblages from 25 individuals with generalized chronic periodontitis and 25 periodontally healthy individuals using whole genome shotgun sequencing. core metabolic networks were computed from taxa and genes identified in at least 80% of individuals in each group. 50% of ...201627991530
dental plaque microbial profiles of children from khartoum, sudan, with congenital heart defects.few studies have focused on the bacterial species associated with the deterioration of the dental and gingival health of children with congenital heart defects (chd). the aims of this study were (1) to examine the dental plaque of children with chd in order to quantify bacterial load and altered bacterial composition compared with children without chd; and (2) to investigate the correlation between the level of caries and gingivitis and dental biofilm bacteria among those children. in this cross ...201728326155
effect of cigarette smoking on subgingival bacteria in healthy subjects and patients with chronic periodontitis.cigarette smoking is known to increase the risk of periodontal destruction and developing chronic periodontitis (cp). it is also reported to affect the subgingival bacterial profile among cp patients. however, studies on the effect of smoking on the bacterial profile among healthy subjects are still limited. therefore, the aim of this study was to investigate the impact of smoking on the subgingival bacterial profile in both healthy adults and cp patients.201728327165
behavior of two tannerella forsythia strains and cell surface mutants in multispecies oral biofilms.as a member of subgingival multispecies biofilms, tannerella forsythia is commonly associated with periodontitis. the bacterium has a characteristic cell surface (s-) layer modified with a unique o-glycan. both the s-layer and the o-glycan were analyzed in this study for their role in biofilm formation by employing an in vitro multispecies biofilm model mimicking the situation in the oral cavity. different t. forsythia strains and mutants with characterized defects in cell surface composition we ...201728382776
common polymorphisms in the ifi16 and aim2 genes are associated with periodontal disease.we investigated the single nucleotide polymorphism (snp) context of a previously identified periodontitis-associated locus and examined its association with microbial, biological and periodontal disease clinical parameters.201728387608
microbiological, lipid and immunological profiles in children with gingivitis and type 1 diabetes mellitus.the aim of this study was to compare the prevalence of periodontal pathogens, systemic inflammatory mediators and lipid profiles in type 1 diabetes children (dm) with those observed in children without diabetes (ndm), both with gingivitis.201728403363
herpes simplex 1 and periopathogen role in peri-implantitis.the objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type i (hsv-1) in implant surfaces between participants with peri-implantitis (pi) and healthy peri-implant tissues and to quantitatively assess the relation between hsv-1 and periopathogens inside the microbiological profile associated with pi.201728512280
linkages between oral commensal bacteria and atherosclerotic plaques in coronary artery disease patients.coronary artery disease is an inflammatory disorder characterized by narrowing of coronary arteries due to atherosclerotic plaque formation. to date, the accumulated epidemiological evidence supports an association between oral bacterial diseases and coronary artery disease, but has failed to prove a causal link between the two. due to the recent surge in microbial identification and analyses techniques, a number of bacteria have been independently found in atherosclerotic plaque samples from co ...201628649401
effects of photodynamic therapy in periodontal treatment: a randomized, controlled clinical trial.to evaluate the effects of photodynamic therapy (pdt) in the nonsurgical treatment of chronic periodontitis.201728667678
periodontal microbioma and rheumatoid arthritis: the role of porhyromonas gingivalis.rheumatoid arthritis is a disease, which can be described as an autoimmune response after molecular mimicry caused by infective agents. the current study aims at evaluating the correlation between rhematoid arthritis (ra) and periodontal disease (pd), with special attention to the microbioma detected in the gums. thirty-four patients with rd were recruited into the current study. among rheumatic parameters, rheumatoid factor (rf), anti-citrullinated protein antibody (ccp), hla-bdr1 and das28 wer ...201728691459
maldi-tof ms contribution to the diagnosis of campylobacter rectus multiple skull base and brain abscesses.campylobacter rectus is rarely associated with invasive infection. both the isolation and the identification requirements of c. rectus are fastidious, probably contributing to an underestimation of its burden. we report the case of a 66-year-old man who developed several skull base and intracerebral abscesses after dental intervention. campylobacter rectus was isolated from the brain biopsy. within 45 minutes of reading the bacterial plate, the strain was accurately identified by maldi-tof ms. t ...201728736616
prevalence of periodontal pathogens among italian patients with chronic periodontitis: a retrospective study on 2992 patients.the aim of the present study was to evaluate the prevalence of some periodontal pathogens in italian adults with chronic periodontitis.201728757933
the oral microbiome and adverse pregnancy outcomes.significant evidence supports an association between periodontal pathogenic bacteria and preterm birth and preeclampsia. the virulence properties assigned to specific oral pathogenic bacteria, for example, fusobacterium nucleatum, porphyromonas gingivalis, filifactor alocis, campylobacter rectus, and others, render them as potential collaborators in adverse outcomes of pregnancy. several pathways have been suggested for this association: 1) hematogenous spread (bacteremia) of periodontal pathoge ...201728848365
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