Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [prevalence of putative periodontal microorganisms in chinese patients with aggressive periodontitis]. | to evaluate the prevalence of putative periodontal microorganism in chinese patients with aggressive periodontitis (agp). | 2006 | 16836903 |
| effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes. | patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ... | 2006 | 16841550 |
| identification of biofilm proteins in non-typeable haemophilus influenzae. | non-typeable haemophilus influenzae biofilm formation is implicated in a number of chronic infections including otitis media, sinusitis and bronchitis. biofilm structure includes cells and secreted extracellular matrix that is "slimy" and believed to contribute to the antibiotic resistant properties of biofilm bacteria. components of biofilm extracellular matrix are largely unknown. in order to identify such biofilm proteins an ex-vivo biofilm of a non-typeable haemophilus influenzae isolate, or ... | 2006 | 16854240 |
| enhanced biofilm formation and loss of capsule synthesis: deletion of a putative glycosyltransferase in porphyromonas gingivalis. | periodontitis is a biofilm-mediated disease. porphyromonas gingivalis is an obligate anaerobe consistently associated with severe manifestations of this disease. as an opportunistic pathogen, the ability to proliferate within and disseminate from subgingival biofilm (plaque) is central to its virulence. here, we report the isolation of a p. gingivalis transposon insertion mutant altered in biofilm development and the reconstruction and characterization of this mutation in three different wild-ty ... | 2006 | 16855241 |
| antibody response after single-visit full-mouth ultrasonic debridement versus quadrant-wise therapy. | the aim of this study was to compare serum antibody responses to periodontal pathogens after single-visit full-mouth ultrasonic debridement and quadrant-wise therapy. | 2006 | 16856899 |
| treponema denticola in disseminating endodontic infections. | treponema denticola is a consensus periodontal pathogen that has recently been associated with endodontic pathology. in this study, the effect of mono-infection of the dental pulp with t. denticola and with polymicrobial "red-complex" organisms (rc) (porphyromonas gingivalis, tannerella forsythia, and t. denticola) in inducing disseminating infections in wild-type (wt) and severe-combined-immunodeficiency (scid) mice was analyzed. after 21 days, a high incidence (5/10) of orofacial abscesses was ... | 2006 | 16861296 |
| identification of smtb/arsr cis elements and proteins in archaea using the prokaryotic intergenic exploration database (piged). | microbial genome sequencing projects have revealed an apparently wide distribution of smtb/arsr metal-responsive transcriptional regulators among prokaryotes. using a position-dependent weight matrix approach, prokaryotic genome sequences were screened for smtb/arsr dna binding sites using data derived from intergenic sequences upstream of orthologous genes encoding these regulators. sixty smtb/arsr operators linked to metal detoxification genes, including nine among various archaeal species, ar ... | 2006 | 16877320 |
| sampling strategy for intraoral detection of periodontal pathogens before and following periodontal therapy. | the aim of this study was to identify a sampling strategy with high probability for detecting oral colonization by actinobacillus actinomycetemcomitans, porphyromonas gingivalis, eikenella corrodens, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, and treponema denticola before and following mechanical periodontal therapy. | 2006 | 16881801 |
| roles of the host oxidative immune response and bacterial antioxidant rubrerythrin during porphyromonas gingivalis infection. | the efficient clearance of microbes by neutrophils requires the concerted action of reactive oxygen species and microbicidal components within leukocyte secretory granules. rubrerythrin (rbr) is a nonheme iron protein that protects many air-sensitive bacteria against oxidative stress. using oxidative burst-knockout (nadph oxidase-null) mice and an rbr gene knockout bacterial strain, we investigated the interplay between the phagocytic oxidative burst of the host and the oxidative stress response ... | 2006 | 16895445 |
| antimicrobial therapy using a local drug delivery system (arestin) in the treatment of peri-implantitis. i: microbiological outcomes. | to assess the microbiological outcome of local administration of minocycline hydrochloride microspheres 1 mg (arestin) in cases with peri-implantitis and with a follow-up period of 12 months. | 2006 | 16907769 |
| arginine-specific gingipains from porphyromonas gingivalis deprive protective functions of secretory leucocyte protease inhibitor in periodontal tissue. | chronic periodontitis is correlated with porphyromonas gingivalis infection. in this study, we found that the expression of secretory leucocyte protease inhibitor (slpi), an endogenous inhibitor for neutrophil-derived proteases, was reduced in gingival tissues with chronic periodontitis associated with p. gingivalis infection. the addition of vesicles of p. gingivalis decreased the amount of slpi in the media of primary human gingival keratinocytes compared to untreated cultures. we therefore in ... | 2006 | 16907925 |
| comparative genome analysis: selection pressure on the borrelia vls cassettes is essential for infectivity. | at least three species of borrelia burgdorferi sensu lato (bbsl) cause tick-borne lyme disease. previous work including the genome analysis of b. burgdorferi b31 and b. garinii pbi suggested a highly variable plasmid part. the frequent occurrence of duplicated sequence stretches, the observed plasmid redundancy, as well as the mainly unknown function and variability of plasmid encoded genes rendered the relationships between plasmids within and between species largely unresolvable. | 2006 | 16914037 |
| association of eubacterium nodatum and treponema denticola with human periodontitis lesions. | the purpose of the present investigation was to compare the levels, proportions and percentage of sites colonized by 40 bacterial species in subgingival plaque samples from periodontally healthy subjects and patients with chronic periodontitis to seek possible pathogens other than the consensus pathogens porphyromonas gingivalis and tannerella forsythia. | 2006 | 16922925 |
| porphyromonas gingivalis vesicles enhance attachment, and the leucine-rich repeat bspa protein is required for invasion of epithelial cells by "tannerella forsythia". | the human oral cavity harbors more than 500 species of bacteria. periodontitis, a bacterially induced inflammatory disease that leads to tooth loss, is believed to result from infection by a select group of gram-negative periodontopathogens that includes porphyromonas gingivalis, treponema denticola, and "tannerella forsythia" (opinion on name change from tannerella forsythensis pending; formerly bacteroides forsythus). epithelial cell invasion by periodontopathogens is considered to be an impor ... | 2006 | 16926393 |
| novel conserved assembly factor of the bacterial flagellum. | tp0658 (fliw) and its orthologs, conserved proteins of unknown function in treponema pallidum and other species, interact with a c-terminal region of flagellin (flab1-3 in t. pallidum; flic in most other species). mutants of orthologs in bacillus subtilis and campylobacter jejuni (yvif, cj1075) showed strongly reduced motility. tp0658 stabilizes flagellin in a way similar to flis, suggesting that tp0658 is a conserved assembly factor for the bacterial flagellum. | 2006 | 16936039 |
| data mining for proteins characteristic of clades. | a synapomorphy is a phylogenetic character that provides evidence of shared descent. ideally a synapomorphy is ubiquitous within the clade of related organisms and nonexistent outside the clade, implying that it arose after divergence from other extant species and before the last common ancestor of the clade. with the recent proliferation of genetic sequence data, molecular synapomorphies have assumed great importance, yet there is no convenient means to search for them over entire genomes. we h ... | 2006 | 16936320 |
| inhibition of human immunodeficiency virus type 1 entry by a binding domain of porphyromonas gingivalis gingipain. | human immunodeficiency virus (hiv) transmission through saliva is extremely low. several oral components, including secretory immunoglobulin a and secretory leukocyte protease inhibitor, are known as potential inhibitory agents of hiv oral transmission. here we examined anti-hiv activity of oral bacterial components. we showed that recombinant protein hgp44 derived from porphyromonas gingivalis, one of the primary infectious agents of periodontitis, was capable of inhibiting hiv type 1 (hiv-1) r ... | 2006 | 16940103 |
| microbiota of the dorsum of the tongue after plaque accumulation: an experimental study in humans. | the purpose of the present investigation was to determine the effect of the absence of tongue hygiene on the microbiota of the dorsum of the tongue. | 2006 | 16945032 |
| levels of matrix metalloproteinases-8 and -9 with simultaneous presence of periodontal pathogens in gingival crevicular fluid as well as matrix metalloproteinase-9 and cholesterol in blood. | to investigate the levels of matrix metalloproteinase (mmp) -8 and -9 with the simultaneous presence of periodontal pathogens in gingival crevicular fluid (gcf) as well as mmp-9 and cholesterol in blood. although bacterial pathogens are required to initiate the periodontal disease process, in some individuals the reaction to bacteria may lead to an excessive host response, resulting in a general inflammatory response. | 2006 | 16953818 |
| molecular epidemiology of oral treponemes in patients with periodontitis and in periodontitis-resistant subjects. | the etiologic role of oral treponemes in human periodontitis is still under debate. although seen by dark-field microscopy in large numbers, their possible role is still unclear since they comprise some 60 different phylotypes, most of which are still uncultured. to determine their status as mere commensals or opportunistic pathogens, molecular epidemiological studies are required that include both cultured and as-yet-uncultured organisms. here we present such data, comparing treponemal populati ... | 2006 | 16954230 |
| detection of cariogenic streptococcus mutans in extirpated heart valve and atheromatous plaque specimens. | the involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. however, no information is available regarding the involvement of cariogenic pathogens such as streptococcus mutans. the presence of oral streptococcal species and periodontitis-related bacteria in 35 ... | 2006 | 16954266 |
| diagnosis of gastric syphilis by direct immunofluorescence staining and real-time pcr testing. | we report on a case of gastric syphilis in a patient with chronic dyspepsia. the diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal dna sequences by a real-time pcr. | 2006 | 16954299 |
| refuting phylogenetic relationships. | phylogenetic methods are philosophically grounded, and so can be philosophically biased in ways that limit explanatory power. this constitutes an important methodologic dimension not often taken into account. here we address this dimension in the context of concatenation approaches to phylogeny. | 2006 | 16956399 |
| periodontal therapy in siblings with papillon-lefèvre syndrome and tinea capitis: a report of two cases. | report of clinical and microbiological periodontal findings before and 6 months after treatment of two siblings with papillon-lefèvre syndrome (pls) and tinea capitis. | 2006 | 16970621 |
| biochemical and molecular characterization of the biosynthesis of glutamine and glutamate, two major compatible solutes in the moderately halophilic bacterium halobacillus halophilus. | the moderately halophilic, chloride-dependent bacterium halobacillus halophilus produces glutamate and glutamine as main compatible solutes at external salinities of 1.0 to 1.5 m nacl. the routes for the biosynthesis of these solutes and their regulation were examined. the genome contains two genes potentially encoding glutamate dehydrogenases and two genes for the small subunit of a glutamate synthase, but only one gene for the large subunit. however, the expression of these genes was not salt ... | 2006 | 16980483 |
| molecular analysis of filifactor alocis, tannerella forsythia, and treponema denticola associated with primary endodontic infections and failed endodontic treatment. | the aim of this study was to investigate the presence of strict anaerobes such as filifactor alocis, tannerella forsythia, and treponema denticola in primary and secondary root-infected canals with periapical lesions by molecular analysis and the association of these species with specific endodontic signs and symptoms. microbial samples were taken from 100 root canals, 50 with necrotic pulp tissues (npt, primary infection), and 50 with failed endodontic treatment (fet, secondary infection). dna ... | 2006 | 16982268 |
| the flagellar cytoskeleton of the spirochetes. | the recent discoveries of prokaryotic homologs of all three major eukaryotic cytoskeletal proteins (actin, tubulin, intermediate filaments) have spurred a resurgence of activity in the field of bacterial morphology. in spirochetes, however, it has long been known that the flagellar filaments act as a cytoskeletal protein structure, contributing to their shape and conferring motility on this unique phylum of bacteria. therefore, revisiting the spirochete cytoskeleton may lead to new paradigms for ... | 2006 | 16983197 |
| structural characterization of peptide-mediated inhibition of porphyromonas gingivalis biofilm formation. | porphyromonas gingivalis is a periodontal pathogen whose primary niche is the anaerobic environment of subgingival dental plaque, but initial colonization of the oral cavity is likely to occur on supragingival surfaces that already support robust biofilm communities. our studies have shown that p. gingivalis adheres to streptococcus gordonii through interaction of the minor fimbrial antigen mfa1 with a specific region of the streptococcal sspb polypeptide (residues 1167 to 1193) designated bar. ... | 2006 | 16988253 |
| oral malodour (halitosis). | 2006 | 16990322 | |
| identification and comparative analysis of sixteen fungal peptidyl-prolyl cis/trans isomerase repertoires. | the peptidyl-prolyl cis/trans isomerase (ppiase) class of proteins is present in all known eukaryotes, prokaryotes, and archaea, and it is comprised of three member families that share the ability to catalyze the cis/trans isomerisation of a prolyl bond. some fungi have been used as model systems to investigate the role of ppiases within the cell, however how representative these repertoires are of other fungi or humans has not been fully investigated. | 2006 | 16995943 |
| periodontal disease and systemic conditions: a bidirectional relationship. | for decades, physicians and dentists have paid close attention to their own respective fields, specializing in medicine pertaining to the body and the oral cavity, respectively. however, recent findings have strongly suggested that oral health may be indicative of systemic health. currently, this gap between allopathic medicine and dental medicine is quickly closing, due to significant findings supporting the association between periodontal disease and systemic conditions such as cardiovascular ... | 2006 | 16998613 |
| identification of mgla-regulated genes reveals novel virulence factors in francisella tularensis. | the facultative intracellular bacterium francisella tularensis causes the zoonotic disease tularemia. f. tularensis resides within host macrophages in vivo, and this ability is essential for pathogenesis. the transcription factor mgla is required for the expression of several francisella genes that are necessary for replication in macrophages and for virulence in mice. we hypothesized that the identification of mgla-regulated genes in the francisella genome by transcriptional profiling of wild-t ... | 2006 | 17000729 |
| the influence of cholesterol, progesterone, 4-androstenedione and testosterone on the growth of treponema denticola atcc 33520 in batch cultures. | previously, we have shown that reference and freshly isolated treponema denticola cultures are capable of metabolising cholesterol, progesterone, 4-androstenedione and testosterone by means of 5alpha-reductase, 3beta-and 17beta-hydroxysteroid dehydrogenase activity [clark dt, soory m. the metabolism of cholesterol and certain hormonal steroids by treponema denticola. steroids. 2006;71:352-63. ]. the aim of the work presented in this paper was to investigate the modulation of t. denticola growth ... | 2006 | 17011804 |
| holo- and apo-cystalysin from treponema denticola: two different conformations. | cystalysin, the key virulence factor in the bacterium treponema denticola responsible for periodontis, is a pyridoxal 5'-phosphate (plp) enzyme which catalyzes, in addition to alpha,beta-elimination of l-cysteine, racemization and transamination of both enantiomers of alanine. in this paper several indicators have been used as probes of the different conformational status of t. denticola cystalysin in the holo and apo form. compared to holoenzyme, the apoenzyme displays an altered reactivity of ... | 2006 | 17014820 |
| denaturing gradient gel electrophoresis as a diagnostic tool in periodontal microbiology. | bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. the aim of the present study was to show the potential of denaturing gradient gel electrophoresis (dgge) as a tool for the detection of clinically relevant species and to compare the results of detection by dgge with those by pcr and culturing. hybridization of the bands from the dgge profiles with species-specific probes was developed ... | 2006 | 17021091 |
| changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16s cloning and sequencing. | the gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. understanding the dynamics of this ecosystem in transitions between health and disease is important in advancing our understanding of the bacterial etiology of periodontitis. the objective of this longitudinal study was to examine the stability of bacterial colonization in the gingival crevice and to explore the relationship between shifts in microbial composition and changes in periodontal health status u ... | 2006 | 17021095 |
| rmlc, a c3' and c5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. | the striking feature of carbohydrates is their constitutional, conformational and configurational diversity. biology has harnessed this diversity and manipulates carbohydrate residues in a variety of ways, one of which is epimerization. rmlc catalyzes the epimerization of the c3' and c5' positions of dtdp-6-deoxy-d-xylo-4-hexulose, forming dtdp-6-deoxy-l-lyxo-4-hexulose. rmlc is the third enzyme of the rhamnose pathway, and represents a validated anti-bacterial drug target. although several stru ... | 2007 | 17046787 |
| rmlc, a c3' and c5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. | the striking feature of carbohydrates is their constitutional, conformational and configurational diversity. biology has harnessed this diversity and manipulates carbohydrate residues in a variety of ways, one of which is epimerization. rmlc catalyzes the epimerization of the c3' and c5' positions of dtdp-6-deoxy-d-xylo-4-hexulose, forming dtdp-6-deoxy-l-lyxo-4-hexulose. rmlc is the third enzyme of the rhamnose pathway, and represents a validated anti-bacterial drug target. although several stru ... | 2007 | 17046787 |
| infection frequency of epstein-barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters. | to detect the infection frequencies of different genotypes of epstein-barr virus (ebv) in subgingival samples from chronic periodontitis (cp) patients, and to discuss the correlation between infection with ebv and clinical parameters. | 2006 | 17048301 |
| [pathogenic potential of porphyromonas gingivalis, treponema denticola and tannerella forsythia, the red bacterial complex associated with periodontitis]. | periodontitis are mixed bacterial infections leading to destruction of tooth-supporting tissues, including periodontal ligament and alveolar bone. among over 500 bacterial species living in the oral cavity, a bacterial complex named "red complex" and made of porphyromonas gingivalis, treponema denticola and tannerella forsythia has been strongly related to advanced periodontal lesions. while periodontopathogenic bacteria are the primary etiologic factor of periodontitis, tissue destruction essen ... | 2007 | 17049750 |
| dynamic evolution of selenocysteine utilization in bacteria: a balance between selenoprotein loss and evolution of selenocysteine from redox active cysteine residues. | selenocysteine (sec) is co-translationally inserted into protein in response to uga codons. it occurs in oxidoreductase active sites and often is catalytically superior to cysteine (cys). however, sec is used very selectively in proteins and organisms. the wide distribution of sec and its restricted use have not been explained. | 2006 | 17054778 |
| carpegen real-time polymerase chain reaction vs. anaerobic culture for periodontal pathogen identification. | the aim of this study was to compare two methods of microbiological diagnosis, anaerobic bacterial culture and real-time polymerase chain reaction (pcr), for the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, fusobacterium nucleatum, and treponema denticola. | 2006 | 17064390 |
| molecular analysis of the subgingival microbiota in health and disease. | this investigation provides molecular analyses of the periodontal microbiota in health and disease. subgingival samples from 47 volunteers with healthy gingivae or clinically diagnosed chronic periodontitis were characterized by pcr-denaturing gradient gel electrophoresis (dgge) with primers specific for the v2-v3 region of the eubacterial 16s rrna gene. a hierarchical dendrogram was constructed from band patterns. all unique pcr amplicons (dgge bands) were sequenced for identity. samples were a ... | 2007 | 17085691 |
| molecular analysis of the subgingival microbiota in health and disease. | this investigation provides molecular analyses of the periodontal microbiota in health and disease. subgingival samples from 47 volunteers with healthy gingivae or clinically diagnosed chronic periodontitis were characterized by pcr-denaturing gradient gel electrophoresis (dgge) with primers specific for the v2-v3 region of the eubacterial 16s rrna gene. a hierarchical dendrogram was constructed from band patterns. all unique pcr amplicons (dgge bands) were sequenced for identity. samples were a ... | 2007 | 17085691 |
| comparison of a taqman-based real-time polymerase chain reaction with conventional tests for the detection of trichomonas vaginalis. | to compare a taqman-based real-time polymerase chain reaction (pcr) with conventional pcr, culture, and wet-mount microscopy for the diagnosis of trichomoniasis in women. | 2007 | 17090567 |
| comparison of a taqman-based real-time polymerase chain reaction with conventional tests for the detection of trichomonas vaginalis. | to compare a taqman-based real-time polymerase chain reaction (pcr) with conventional pcr, culture, and wet-mount microscopy for the diagnosis of trichomoniasis in women. | 2007 | 17090567 |
| identification of the gene encoding the fhbb protein of treponema denticola, a highly unique factor h-like protein 1 binding protein. | the gene encoding the treponema denticola factor h-like protein 1 (fhl-1) binding protein, fhbb, was recovered and characterized. sequence conservation, expression, and properties of fhbb were analyzed. the identification of fhbb represents an important step in understanding the contribution of fhl-1 binding in t. denticola pathogenesis and in development of periodontal disease. | 2007 | 17101650 |
| dimerization and folding processes of treponema denticola cystalysin: the role of pyridoxal 5'-phosphate. | cystalysin, the key virulence factor in the bacterium treponema denticola responsible for periodontitis, is a homodimeric pyridoxal 5'-phosphate (plp)-c-s lyase. the dimerization process and the urea-induced unfolding equilibrium of holocystalysin were compared with those of the apo form. the presence of plp decreases approximately 4 times the monomer-dimer equilibrium dissociation constant. by using a variety of spectroscopic and analytical procedures, we demonstrated a difference in their unfo ... | 2006 | 17115709 |
| the glycolytic pathway of trimastix pyriformis is an evolutionary mosaic. | glycolysis and subsequent fermentation is the main energy source for many anaerobic organisms. the glycolytic pathway consists of ten enzymatic steps which appear to be universal amongst eukaryotes. however, it has been shown that the origins of these enzymes in specific eukaryote lineages can differ, and sometimes involve lateral gene transfer events. we have conducted an expressed sequence tag (est) survey of the anaerobic flagellate trimastix pyriformis to investigate the nature of the evolut ... | 2006 | 17123440 |
| how phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria. | the phosphoenolpyruvate(pep):carbohydrate phosphotransferase system (pts) is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, amino sugars, polyols, and other sugar derivatives. to carry out its catalytic function in sugar transport and phosphorylation, the pts uses pep as an energy source and phosphoryl donor. the phosphoryl group of pep is usually transferred via four distinct proteins (domains) to the transported sugar bo ... | 2006 | 17158705 |
| characterization of the thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase cher:mcp tethering. | sensory adaptation in bacterial chemotaxis is mediated by covalent modifications of specific glutamate and glutamine residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins (mcps). in escherichia coli and salmonella enterica, efficient methylation of mcps depends on the localization of methyltransferase cher to mcp clusters through an interaction between the cher beta-subdomain and a pentapeptide sequence (nwetf or nwesf) at the c-terminus of the mcp. in vitro methylation ... | 2007 | 17163981 |
| characterization of the thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase cher:mcp tethering. | sensory adaptation in bacterial chemotaxis is mediated by covalent modifications of specific glutamate and glutamine residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins (mcps). in escherichia coli and salmonella enterica, efficient methylation of mcps depends on the localization of methyltransferase cher to mcp clusters through an interaction between the cher beta-subdomain and a pentapeptide sequence (nwetf or nwesf) at the c-terminus of the mcp. in vitro methylation ... | 2007 | 17163981 |
| antibodies to periodontal organisms are associated with decreased kidney function. the dental atherosclerosis risk in communities study. | increasing evidence suggests that clinical signs of periodontal disease are independently associated with renal impairment. however, no studies have examined the possible linkage of kidney disease with serum antibody to oral pathogens. | 2007 | 17170550 |
| identification of specific chemoattractants and genetic complementation of a borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay. | measuring the chemotactic response of borrelia burgdorferi, the bacterial species that causes lyme disease, is relatively more difficult than measuring that of other bacteria. because these spirochetes have long generation times, enumerating cells that swim up a capillary tube containing an attractant by using colony counts is impractical. furthermore, direct counts with a petroff-hausser chamber is problematic, as this method has a low throughput and necessitates a high cell density; the latter ... | 2007 | 17172459 |
| identification of specific chemoattractants and genetic complementation of a borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay. | measuring the chemotactic response of borrelia burgdorferi, the bacterial species that causes lyme disease, is relatively more difficult than measuring that of other bacteria. because these spirochetes have long generation times, enumerating cells that swim up a capillary tube containing an attractant by using colony counts is impractical. furthermore, direct counts with a petroff-hausser chamber is problematic, as this method has a low throughput and necessitates a high cell density; the latter ... | 2007 | 17172459 |
| phylogenetic analysis of bacterial and archaeal species in symptomatic and asymptomatic endodontic infections. | phylogenetic analysis of bacterial and archaeal 16s rrna was used to examine polymicrobial communities within infected root canals of 20 symptomatic and 14 asymptomatic patients. nucleotide sequences from approximately 750 clones amplified from each patient group with universal bacterial primers were matched to the ribosomal database project ii database. phylotypes from 37 genera representing actinobacteria, bacteroidetes, firmicutes, fusobacteria and proteobacteria were identified. results were ... | 2007 | 17172525 |
| prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations. | evidence is mounting that the prevalence of some putative endodontic pathogens can significantly vary according to the geographic location in which samples were taken. this study aimed to provide additional knowledge on this subject by comparing the frequencies of 10 selected candidate endodontic pathogens in samples of acute apical abscesses obtained from two distinct geographic locations; portland, or, and rio de janeiro, brazil. dna was extracted from aspirates and used as template in nested ... | 2006 | 17174667 |
| characterization of treponema denticola pyrf encoding orotidine-5'-monophosphate decarboxylase. | the treponema denticola atcc 35405 genome annotation contains most of the genes for de novo pyrimidine biosynthesis. to initiate characterization of pyrimidine synthesis in treponema, we focused on tde2110 (the putative pyrf, encoding orotidine-5'-monophosphate decarboxlyase). unlike the parent strain, an isogenic pyrf mutant was resistant to 5-fluoroorotic acid. in complex medium, growth of the pyrf mutant was independent of added uracil, indicating activity of a uracil uptake/salvage pathway. ... | 2007 | 17187656 |
| the essential nature of sphingolipids in plants as revealed by the functional identification and characterization of the arabidopsis lcb1 subunit of serine palmitoyltransferase. | serine palmitoyltransferase (spt) catalyzes the first step of sphingolipid biosynthesis. in yeast and mammalian cells, spt is a heterodimer that consists of lcb1 and lcb2 subunits, which together form the active site of this enzyme. we show that the predicted gene for arabidopsis thaliana lcb1 encodes a genuine subunit of spt that rescues the sphingolipid long-chain base auxotrophy of saccharomyces cerevisiae spt mutants when coexpressed with arabidopsis lcb2. in addition, homozygous t-dna inser ... | 2006 | 17194770 |
| two subgingival plaque-sampling strategies used with rna probes. | the purpose of this study was to compare the results of microbiological rna-probe analysis after subgingival plaque sampling applying two different strategies. | 2007 | 17199542 |
| rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease. | one of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. porphyromonas gingivalis, treponema denticola, and tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. in this study we hypothesized that p. gingivalis, t. denticola, and t. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in ... | 2007 | 17210663 |
| phylogenetic distribution of translational gtpases in bacteria. | translational gtpases are a family of proteins in which gtpase activity is stimulated by the large ribosomal subunit. conserved sequence features allow members of this family to be identified. | 2007 | 17214893 |
| fatty acids synthesized by oral treponemes in chemically defined media. | omiz-w68, a chemically defined medium that contains no long-chain fatty acids and yet supports in vitro proliferation of a wide range of fastidious oral anaerobes, is described. the type strains of treponema denticola, treponema lecithinolyticum, treponema maltophilum, treponema pectinovorum, treponema socranskii, and an as yet unpublished canine treponema species could be propagated indefinitely in this medium with sugar supplements for the saccharolytic species. analysis of the cellular fatty ... | 2007 | 17229060 |
| identification of the microorganisms responsible for periodontopathy by multiplex rt-pcr. | the aim of our research was to identify by bacterial genomic dna analysis the prevalence of five different species of periodontopathogenic bacteria present in the subgingival biofilm, specifically: actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), bacterioides forsytus (bf), treponema denticola (td). for the analysis we used the systematic multiplex-pcr-microdent kit with species-specific primers. we studied a group of 48 subjects, 18 males and ... | 2006 | 17263160 |
| association between infection of different strains of porphyromonas gingivalis and actinobacillus actinomycetemcomitans in subgingival plaque and clinical parameters in chronic periodontitis. | the aim of this study was to investigate subgingival infection frequencies of porphyromonas gingivalis and actinobacillus actinomycetemcomitans strains with genetic variation in chinese chronic periodontitis (cp) patients and to evaluate its correlation with clinical parameters. | 2007 | 17266188 |
| evaluation of the incidence of periodontitis-associated bacteria in the atherosclerotic plaque of coronary blood vessels. | unstable atherosclerotic plaque is a dangerous clinical condition, possibly leading to acute coronary deficiency resulting in cardiac infarction. questions about the role of inflammatory factors in the formation of pathological lesions in the endothelium of coronary vessels have often been raised. this condition may be caused by bacteria that are able to initiate clot formation in a blood vessel, destabilizing an atherosclerotic plaque that is already present. the sources of these pathogens are ... | 2007 | 17274722 |
| genome of the opportunistic pathogen streptococcus sanguinis. | the genome of streptococcus sanguinis is a circular dna molecule consisting of 2,388,435 bp and is 177 to 590 kb larger than the other 21 streptococcal genomes that have been sequenced. the g+c content of the s. sanguinis genome is 43.4%, which is considerably higher than the g+c contents of other streptococci. the genome encodes 2,274 predicted proteins, 61 trnas, and four rrna operons. a 70-kb region encoding pathways for vitamin b(12) biosynthesis and degradation of ethanolamine and propanedi ... | 2007 | 17277061 |
| intermolecular forces and enthalpies in the adhesion of streptococcus mutans and an antigen i/ii-deficient mutant to laminin films. | the antigen i/ii family of surface proteins is expressed by most oral streptococci, including streptococcus mutans, and mediates specific adhesion to, among other things, salivary films and extracellular matrix proteins. in this study we showed that antigen i/ii-deficient s. mutans isogenic mutant ib03987 was nearly unable to adhere to laminin films under flow conditions due to a lack of specific interactions (0.8 x 10(6) and 1.1 x 10(6) cells cm(-2) at ph 5.8 and 6.8, respectively) compared wit ... | 2007 | 17277062 |
| physiological osmotic induction of leptospira interrogans adhesion: liga and ligb bind extracellular matrix proteins and fibrinogen. | transmission of leptospirosis occurs through contact of mucous membranes and abraded skin with freshwater contaminated by pathogenic leptospira spp. exposure to physiological osmolarity induces leptospires to express high levels of the lig surface proteins containing imperfect immunoglobulin-like repeats that are shared or differ between liga and ligb. we report that osmotic induction of lig is accompanied by 1.6- to 2.5-fold increases in leptospiral adhesion to immobilized extracellular matrix ... | 2007 | 17296754 |
| novel tetracycline resistance determinant isolated from an environmental strain of serratia marcescens. | resistances to tetracycline and mercury were identified in an environmental strain of serratia marcescens isolated from a stream highly contaminated with heavy metals. as a step toward addressing the mechanisms of coselection of heavy metal and antibiotic resistances, the tetracycline resistance determinant was cloned in escherichia coli. within the cloned 13-kb segment, the tetracycline resistance locus was localized by deletion analysis and transposon mutagenesis. dna sequence analysis of an 8 ... | 2007 | 17308196 |
| microbiological outcomes of quadrant versus full-mouth root planing as monitored by real-time pcr. | to study the short-term microbiological changes following full-mouth compared with quadrant wise scaling and root planing (fmrp and qrp) as well as long-term effects. | 2007 | 17309590 |
| subgingival plaque microbiota in hiv positive patients. | to describe and compare the predominant bacterial and fungal species associated with gingivitis, periodontitis, and linear gingival erythema (lge), in hiv positive subjects with different immune status. | 2007 | 17309593 |
| a comparison of implant-supported, bar- or ball-retained mandibular overdentures: a retrospective clinical, microbiologic, and immunologic study of 10 edentulous patients attending a recall visit. | clinical, microbiologic, and immunologic comparisons of the peri-implant health in edentulous volunteers wearing long-standing implant-supported ball- or dolder bar-retained mandibular overdentures were performed. | 2007 | 17319360 |
| mechanisms of decreased susceptibility to beta-defensins by treponema denticola. | treponema denticola, a periodontal pathogen, is relatively resistant to human beta-defensins, which are small cationic antimicrobial peptides produced by a number of cells, including the gingival epithelium. using two independent methods, we previously demonstrated that t. denticola proteases are not responsible for decreased vulnerability to defensins. in this study, we confirmed that the major outer membrane protease, dentilisin, is not responsible for t. denticola insensitivity to defensins a ... | 2007 | 17325047 |
| subgingival chlorhexidine varnish administration as an adjunct to same-day full-mouth root planing. ii. microbiological observations. | recently, the clinical additive value of a highly concentrated chlorhexidine (chx) varnish, when applied subgingivally as an adjunct to scaling and root planing, was described. the objective of the present study was to investigate the microbiological impact of a treatment strategy for chronic periodontitis based on a combination of same-day full-mouth root planing and subgingival chx varnish administration. | 2007 | 17335366 |
| flow cytometric analysis of adherence of porphyromonas gingivalis to oral epithelial cells. | by using fluorescence microscopy, fluorescently labeled porphyromonas gingivalis w50 was shown to adhere to oral epithelial (kb) cells as discrete cells or small cell aggregates, whereas p. gingivalis atcc 33277 bound as large cell aggregates. flow cytometric analysis showed that for p. gingivalis w50 there was a logarithmic relationship between the bacterial cell ratio (bcr), that is the number of bacterial cells to kb cells, and the percentage of kb cells with w50 cells attached. this percenta ... | 2007 | 17339349 |
| activation of mapk in fibroblasts by treponema denticola major outer sheath protein. | the major outer sheath protein (msp) of treponema denticola induces ca(2+) entry and actin reorganization in cultured fibroblasts, but the pathways by which msp mediates these responses are not yet defined. we considered that msp may activate protein kinases as a stress response that precedes actin remodelling. phospho-kinase screens showed that msp induced phosphorylation of multiple kinases in pathways that respond to extracellular agonists and regulate actin assembly. 34 kinases were signific ... | 2007 | 17346673 |
| the detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study. | periodontal disease is a commonly prevalent problem faced alike by both the developed and third world countries but showing wide variations in prevalence and severity across different geographical areas. the purpose was to identify actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), ekinella corrodens (ec), campylobacter rectus (cr), bacteroides forsythus (bf), treponema denticola (td) and fusobacterium nucleatum (fn) in indian adult periodontiti ... | 2007 | 17347537 |
| a novel prokaryotic trans-2-enoyl-coa reductase from the spirochete treponema denticola. | an nadh-dependent trans-2-enoyl-coa reductase (ec1.1.1.36) from the gram negative spirochete treponema denticola was identified, expressed and biochemically characterized. the recombinant protein is a monomeric enzyme with a molecular mass of 44 kda with a specific activity of 43+/-4.8 u/mg (micromol mg(-1)min(-1)) and k(m) value of 2.7 microm for crotonoyl-coa. this nadh-dependent trans-2-enoyl-coa reductase represents the first enzymatically characterized member of a prokaryotic protein family ... | 2007 | 17382934 |
| evidence of periopathogenic microorganisms in placentas of women with preeclampsia. | periodontal disease is a chronic inflammatory infectious disease that may act as a focus of infection. preeclampsia is a pregnancy-specific hypertensive disorder that often leads to maternal morbidity and mortality. acute atherosis, the placental lesion of preeclampsia, shares many histopathological features with atherosclerosis. recently, chronic infection was linked to the initiation of atherosclerosis. oral pathogens have been detected in atherosclerotic plaques, where they may play a role in ... | 2007 | 17397314 |
| prevalence of four putative periodontopathic bacteria in saliva of a group of brazilian children with mixed dentition: 1-year longitudinal study. | this study aimed at evaluating the prevalence of putative periodontal pathogens (actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella nigrescens, treponema denticola) in saliva of children with mixed dentition at two different time points, correlating these findings with a clinical parameter of gingival health. | 2007 | 17397463 |
| transcriptional regulatory network discovery via multiple method integration: application to e. coli k12. | transcriptional regulatory network (trn) discovery from one method (e.g. microarray analysis, gene ontology, phylogenic similarity) does not seem feasible due to lack of sufficient information, resulting in the construction of spurious or incomplete trns. we develop a methodology, trnd, that integrates a preliminary trn, microarray data, gene ontology and phylogenic similarity to accurately discover trns and apply the method to e. coli k12. the approach can easily be extended to include other me ... | 2007 | 17397539 |
| intrinsic apoptotic pathways of gingival epithelial cells modulated by porphyromonas gingivalis. | porphyromonas gingivalis can inhibit chemically induced apoptosis in primary cultures of gingival epithelial cells through blocking activation of the effector caspase-3. the anti-apoptotic phenotype of p. gingivalis is conserved across strains and does not depend on the presence of fimbriae, as fimbriae-deficient mutants and a naturally occurring non-fimbriated strain were able to impede apoptosis. to dissect the survival pathways modulated by p. gingivalis, protein and gene expression of a numb ... | 2007 | 17419719 |
| aggregatibacter actinomycetemcomitans as indicator for aggressive periodontitis by two analysing strategies. | to compare the subgingival microbiota of aggressive and chronic periodontitis (chp) using single-site and pooled plaque samples. | 2007 | 17433043 |
| oral fluid-based biomarkers of alveolar bone loss in periodontitis. | periodontal disease is a bacteria-induced chronic inflammatory disease affecting the soft and hard supporting structures encompassing the teeth. when left untreated, the ultimate outcome is alveolar bone loss and exfoliation of the involved teeth. traditional periodontal diagnostic methods include assessment of clinical parameters and radiographs. though efficient, these conventional techniques are inherently limited in that only a historical perspective, not current appraisal, of disease status ... | 2007 | 17435132 |
| performance of captia selectsyph-g enzyme-linked immunosorbent assay in syphilis testing of a high-risk population: analysis of discordant results. | using the captia select syph-g enzyme-linked immunosorbent assay (elisa), we analyzed the sera of 1,771 patients from a high-risk population attending sexually transmitted disease (std) clinics. we focused on discrepancies between the results of the immunoglobulin g (igg) elisa and the treponema pallidum hemagglutination (tpha) test. we identified 57 patients (3.22%) with conflicting results in the igg elisa and tpha test. in order to resolve these discrepancies, these patients' health records w ... | 2007 | 17442795 |
| identification of intracellular oral species within human crevicular epithelial cells from subjects with chronic periodontitis by fluorescence in situ hybridization. | interactions between oral bacteria and gingival epithelial cells play an important role in the pathogenesis of periodontal diseases. this study used in situ hybridization with 16 rrna probes and confocal microscopy to detect the periodontal pathogens porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythia, and treponema denticola within epithelial cells from periodontal pockets, gingival crevice, and buccal mucosa collected from subjects with chronic periodontitis (n ... | 2007 | 17451543 |
| periodontal pathogens in periodontal pockets and in carotid atheromatous plaques. | in the last years the relationship between periodontitis and cardiovascular diseases has been a subject of increasing research. the identification of periodontopathic bacteria in atheromas can contribute to our knowledge of such an association. the aim of our study was to assess the concomitant presence of 5 periodontal pathogens (actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, treponema denticola, and tannerella forsythia) in periodontal pockets and in car ... | 2007 | 17452955 |
| periodontitis as a risk factor of coronary heart diseases? | unstable atherosclerotic plaque is a dangerous clinical state, possibly leading to acute coronary deficiency resulting in cardiac infarction. inflammatory factor's role in creating pathological lesions in the endothelium of coronary vessels is frequently raised. this state may be caused by bacteria able to initiate clot formation in blood vessel and destabilizing atherosclerotic plaque already present. source of these pathogens are chronic inflammatory processes occurring in organism, among them ... | 2006 | 17458056 |
| periopathogenic bacteria in persistent periapical lesions: an in vivo prospective study. | the aim of this in vivo prospective study was to detect the presence of periopathogenic bacteria in persistent periapical lesions and to compare the reliability of two different methods: anaerobic culture and the dna hybridization technique. | 2007 | 17470025 |
| comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. | the outcome of microbiological diagnostics may depend on the sampling technique. it was the aim of the present study to compare two widely used sampling techniques for subgingival bacteria using quantitative real-time polymerase chain reaction. | 2007 | 17470026 |
| herpesviral-bacterial synergy in the pathogenesis of human periodontitis. | periodontitis is an infectious disease, but the specific mechanisms by which tooth-supportive tissues are lost remain obscure. this article proposes an infectious disease model for periodontitis in which herpesviral-bacterial interactions assume a major etiopathogenic role. | 2007 | 17471038 |
| nursing home-associated pneumonia, hospital-acquired pneumonia and ventilator-associated pneumonia: the contribution of dental biofilms and periodontal inflammation. | 2007 | 17474932 | |
| an in vitro biofilm model of subgingival plaque. | numerous biofilm models have been described for the study of bacteria associated with the supragingival plaque. however, there are fewer models available for the study of subgingival plaque. the purpose of this study was to develop and validate a model that closely mimicked the composition of the subgingival flora. | 2007 | 17488440 |
| the microbiological profiles of saliva, supragingival and subgingival plaque and dental caries in adults with and without type 2 diabetes mellitus. | the relationships between suspected bacteria in saliva, yeasts in oral rinse, and supragingival and subgingival plaque versus root surface and coronal caries in adults with type 2 diabetes mellitus and a non-diabetic group were explored. | 2007 | 17488443 |
| bacterial colonization immediately after installation on oral titanium implants. | information on bacterial colonization immediately after dental implant insertion is limited. | 2007 | 17501978 |
| the crisprdb database and tools to display crisprs and to generate dictionaries of spacers and repeats. | in archeae and bacteria, the repeated elements called crisprs for "clustered regularly interspaced short palindromic repeats" are believed to participate in the defence against viruses. short sequences called spacers are stored in-between repeated elements. in the current model, motifs comprising spacers and repeats may target an invading dna and lead to its degradation through a proposed mechanism similar to rna interference. analysis of intra-species polymorphism shows that new motifs (one spa ... | 2007 | 17521438 |
| treponema denticola induces interleukin-8 and macrophage chemoattractant protein 1 production in human umbilical vein epithelial cells. | treponema denticola, a major pathogen of periodontitis, has also been detected in the lesions of atherosclerosis. the aim of this study was to investigate induction of chemokine production in human umbilical vein endothelial cells (huvecs) by t. denticola and determine whether those chemokines were degraded by a protease, dentilisin. t. denticola atcc35405 or dentilisin-deficient mutant k1 were added to huvecs and levels of interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) in t ... | 2007 | 17533151 |
| microbiological findings after periodontal therapy using curettes, er:yag laser, sonic, and ultrasonic scalers. | to evaluate and compare the microbiological effects of hand instruments, er:yag-laser, sonic, and ultrasonic scalers in patients with chronic periodontitis. patient perception of each treatment was documented. | 2007 | 17555412 |
| an actin-stabilizing peptide conjugate deduced from the major outer sheath protein of the bacterium treponema denticola. | a synthetic peptide conjugated to bovine serum albumin, p34(bsa), based on a 10-mer in the deduced amino acid sequence of the major outer sheath protein of treponema denticola, was found to stabilize actin filaments of fibroblasts. pretreatment of cells with p34(bsa) inhibited the actin disruption induced by cytochalasin d and latrunculin b. p34(bsa) was taken up by the cells and localized among actin filaments. p34(bsa) bound actin from fibroblast lysates, and cell exposure to p34(bsa) led to t ... | 2007 | 17565753 |