Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| in vitro immunization of human b lymphocytes with cultured melanoma cells (sk-mel 28). | human peripheral blood lymphocytes (pbl) from 10 healthy donors were in vitro immunized using the human melanoma cell line sk-mel 28 as an immunogen. the parameters necessary to obtain an optimal immune response against the tumor cells in vitro were determined. different ratios of pbl and sk-mel 28 were tested during the in vitro immunization period, and their effect on cell viability, specific antibody secretion, and yield of antigen-specific ebv clones was determined. a statistically significa ... | 1990 | 1966473 |
| detection of the complement (cd21)/epstein-barr virus receptor in human lacrimal gland and ocular surface epithelia. | cd21, the receptor for the c3d complement fragment, has been reported to be the epstein-barr virus (ebv) receptor in b lymphocytes and cultured squamous epithelial cells. this receptor has previously been found to be expressed in the epithelia of nonocular mucosal-associated lymphoid tissues (malt) which are also sites of persistent ebv infection. we recently found evidence of persistent ebv infection in human ocular malt and hypothesized that the epithelia in these tissues may also express the ... | 1990 | 1966579 |
| normal suppressive t cell function of epstein-barr virus induced b cell activation in type 1 (insulin dependent) diabetes mellitus. | several studies have demonstrated abnormalities of t cell regulation of epstein-barr virus-induced b cell activation in systemic autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematous, and systemic sclerosis. however, a normal suppressive peripheral t cell function was observed in graves' disease. to investigate whether this abnormality is a common feature to other autoimmune diseases, we studied t cell regulation of epstein-barr virus induced b cell activation in 15 newly ... | 1990 | 1966582 |
| expression of hbv envelope and nucleocapsid proteins in human cells using vaccinia virus vectors. | we have analysed the expression of hbv envelope (large, middle, major) and nucleocapsid (core and precore) proteins in human skin fibroblasts and epstein-barr virus-transformed human b-cell lines infected with recombinant vaccinia virus vectors. the large envelope protein was not secreted and barely detectable in the cytosol, whereas middle s and major s where both water soluble and efficiently secreted in abundant quantities. the precore polypeptide was also secreted whereas the core protein wa ... | 1990 | 1966705 |
| [importance of hairy leukoplakia and aids to the dentist. (review of the literature)]. | aids is currently one of the most important and concerning diseases for dentists, both general and specialized. this review of the literature makes emphasis on the main biological, epidemiological, diagnostic and prognostic aspects of oral hairy leucoplakia which every dental care professionist should know, as well as the importance of those conditions in the evolutionary course of this disease. | 1990 | 1966738 |
| combination of mutant el-4 thymoma cells and ebv-infection in b lymphocyte activation a) ebv-infection of el-4-activated lymphocytes. b) use of el-4 cells as feeders for lymphoblastoid cell lines. | the combination of mutant el-4 thymoma cells and epstein-barr virus (ebv)-infection in the activation of human b lymphocytes was studied with two approaches: a) limiting numbers of b cells were first activated by el-4 contact in order to expand the b cell population and then infected with ebv. results show that ebv could induce further ig synthesis, although was unable to determine proliferation or to generate immortalized lines from el-4 activated cells. b) el-4 cells were compared to conventio ... | 1990 | 1966941 |
| hairy leukoplakia: diagnosis and significance. | 1990 | 1967097 | |
| differential expression of intercellular adhesion molecule 1 in primary and metastatic melanoma lesions. | immunochemical studies have shown that the monoclonal antibody (moab) cl203.4, elicited with immune interferon treated cultured human melanoma cells colo 38, recognizes intercellular adhesion molecule 1 (icam-1). the determinant defined by moab cl203.4 is distinct and spatially distant from that defined by anti-icam-1 moab rr1/1, which had been elicited with epstein-barr virus-transformed b-lymphocytes from a lymphocyte function associated antigen 1 deficient patient. immunohistochemical testing ... | 1990 | 1967552 |
| epstein-barr virus infection as a cause of infantile spasms. | 1990 | 1968164 | |
| identification of ebv-dna in lymph nodes from patients with lymphadenopathy and lymphomas associated with aids. | fourteen examples of non-hodgkin's lymphoma (nhl) and four of hodgkin's disease in patients with aids as well as lymph nodes exhibiting changes related to the lymphadenopathy syndrome (las) from 11 hiv-positive individuals were studied for the presence of epstein-barr virus (ebv) genome both by in situ dna hybridization and blotting techniques. both methods were performed using formalin-fixed paraffin-embedded material. all the nhls were of high malignancy and all but one were of the b-cell type ... | 1990 | 1970681 |
| expression of lfa-1 by a lymphoblastoid cell line from a patient with monosomy 21: effects on intercellular adhesion. | monosomy 21 (m21) is a rare aneuploid condition which in certain cases leads to reduced levels of chromosome 21 gene products. we have prepared an epstein-barr virus lymphoblastoid cell-line (lcl) from patient with m21 who has immunological abnormalities, and analysed the expression of lymphocyte function-associated antigen-1 (lfa-1). this heterodimeric leucocyte integrin consists of cd11a (alpha) subunits non-covalently associated with cd18 (beta) subunits coded, respectively, by genes on chrom ... | 1990 | 1975779 |
| characterization of epstein-barr virus-induced lymphoproliferation derived from human peripheral blood mononuclear cells transferred to severe combined immunodeficient mice. | mice with severe combined immunodeficiency (scid) received 6 x 10(7) fresh human peripheral blood mononuclear cells (pbmc) intraperitoneally from epstein-barr virus (ebv)-seropositive and -seronegative donors. b95-8 ebv was inoculated intraperitoneally and intravenously to the mice 6 weeks after transfer of seronegative pbmc. three of four mice transferred with pbmc from two ebv-seropositive donors and two of four mice from two ebv-seronegative donors inoculated with ebv developed fatal ebv-indu ... | 1990 | 1975985 |
| retroviral-mediated gene transfer of the leukocyte integrin cd18 subunit. | children with leukocyte adherence deficiency (lad) exhibit heterogeneous defects in the leukocyte integrin cd18 subunit that prevent surface expression of functional cd11/cd18 leukocyte integrin adherence complexes. we used a retroviral vector, designated lcd18sn, to transfer the cd18 cdna into k562 human myeloid leukemia cells and into ebv b-cells from a child with lad. transfer of the lcd18sn retroviral construct, which expresses the cd18 cdna from the moloney murine leukemia virus (momlv) lon ... | 1990 | 1976304 |
| presence of epstein-barr virus dna in nasal lymphomas of b and 't' cell type. | we studied 12 tumours from 11 chinese patients with primary nasal lymphoma for presence of epstein-barr virus (ebv) dna, using southern-blot analysis. these results were correlated with immunophenotype and t-cell receptor (tcr) or immunoglobulin gene rearrangement patterns. ebv dna was detected in all nine tumours with a 't' phenotype, in both primary and secondary sites. when the structure of the viral genomic termini was studied using the ecori-dhet probe, a single clonal episomal band was dem ... | 1990 | 1979042 |
| epidemiology, clinical features and prognostic value of hiv-1 related oral lesions. | between february 1987 and february 1990, we studied 737 antibody anti hiv-1 positive (abhiv+) subjects referred to the infectious diseases institute of the university of turin (italy) in order to evaluate types, prevalences, relations with clinical stages, distributions in risk-groups and prognostic significances of hiv-1 related oral lesions. the study evidenced the high prevalence of oral lesions, especially mycoses, in the investigated population: 40.3% of the patients showed, in fact, hiv-1 ... | 1990 | 1981079 |
| [mononucleosis syndromes]. | 1990 | 1983304 | |
| a monoclonal antibody with broad anti-hla-dr activity fails to bind to drw11/dw5: possible effect of a unique polymorphism on the beta 1 domain alpha-helix. | we describe the generation and characterization of a murine monoclonal antibody with broad anti-hla-dr beta activity, but which does not recognize drw11/dw5. a balb/c mouse was immunized with a human alloreactive t-cell clone, with the intention of generating monoclonal anti-t-cell-receptor antibodies. in the course of screening the resulting hybridomas, a clone was detected which secreted an antibody (mp2) with anti-hla-dr activity. this was shown by flow cytometry as well as immunoprecipitatio ... | 1991 | 2055782 |
| molecular cloning of cd44r1 and cd44r2, two novel isoforms of the human cd44 lymphocyte "homing" receptor expressed by hemopoietic cells. | in addition to the 85-95 kd cd44 species found on most hemopoietic cell types, the human myelomonocytic cell line kg1a expresses proteins of approximately 115 kd and 130 kd that react with monoclonal antibodies belonging to cd44. the possibility that these higher molecular weight species may represent novel cd44 isoforms containing additional protein sequence was investigated. cd44 cdna clones were isolated from a plasmid-based expression library prepared from kg1a mrna. one of the three clones ... | 1991 | 2056274 |
| potential role of pha in producing human monoclonal thyroid autoantibodies of different subclasses. | a human monoclonal autoantibody to thyroglobulin (tg) of subclass igg2 was developed by fusing a mouse myeloma with tg antibody secreting epstein-barr virus (ebv)-infected b lymphocytes from a hashimoto patient. subsequent studies showed that ebv-infected b lymphocytes from this patient synthesized igg2 tg antibody while unfractionated blood lymphocytes cultured with pokeweed mitogen secreted igg1, igg2, and igg4 tg antibodies in amounts proportional to those present in the patient's serum. to i ... | 1990 | 2103361 |
| the role of human interleukin-6 in b-cell isotype regulation and differentiation. | human interleukin-6 (il-6) is a cytokine secreted by t cells, as well as a variety of other cell types, which exhibits b-cell differentiating activity. the recent cloning of the gene that codes for this molecule has allowed us the opportunity to study the function of this molecule alone and in conjunction with other lymphokines in human b-cell isotype-regulation and differentiation. recombinant human il-6 enhances immunoglobulin (ig) m and g secretion by b-cells activated by staphylococcal a cow ... | 1990 | 2104575 |
| immunoregulation in the common marmoset, calithrix jaccus: functional properties of t and b lymphocytes and their response to human interleukins 2 and 4. | non-human primates have been used to study immune function to a much lesser extent than readily available strains of inbred rodents. nevertheless, in situations where it might be desirable, but impossible, to study human immune responses in vivo, lower primates could provide an acceptable alternative. in order to extent the knowledge of t- and b-lymphocyte function in lower primates, the common marmoset callithrix jaccus was used as an experimental model. the functional similarities between this ... | 1990 | 2110549 |
| viral hepatitis. the new abc's. | hepatitis may be caused by hepatitis a virus, hepatitis b virus, hepatitis c virus (classic non-a non-b viral hepatitis), hepatitis d virus (delta agent), and hepatitis e virus (epidemic non-a non-b viral hepatitis). cytomegalovirus, epstein-barr virus, and herpes simplex virus may also occasionally cause hepatitis. some forms of hepatitis carry the risks of chronic infection, cirrhosis, or hepatocellular carcinoma. treatment options for viral hepatitis are limited and, in many cases, still unde ... | 1990 | 2120686 |
| cloning and sequencing of human lambda immunoglobulin genes by the polymerase chain reaction. | universal oligonucleotide primers, designed for amplifying and sequencing genes encoding the rearranged human lambda immunoglobulin variable region, were validated by amplification of the lambda light chain genes from four human heterohybridoma cell lines and in the generation of a cdna library of human v lambda sequences from epstein-barr virus-transformed human peripheral blood lymphocytes. this technique allows rapid cloning and sequencing of human immunoglobulin genes, and has potential appl ... | 1990 | 2125272 |
| the antibody repertoire of early human b cells. iii. expression of cross-reactive idiotopes characteristic of certain rheumatoid factors and identifying v kappa iii, vhi, and vhiii gene family products. | our previous studies have shown that a high frequency of epstein-barr virus (ebv)-immortalized cord blood (cb) and fetal liver (fl) clones produce igm antibodies which display extensive autoreactivity for igg fc (rheumatoid factor, rf). to investigate further the repertoire of these early b cells, we have examined the expression of cri associated with rf paraproteins in relation to antibody specificity and polyreactivity. cri were detected by elisa and/or flow cytometry using a panel of well-cha ... | 1990 | 2125365 |
| human herpesvirus 6 is closely related to human cytomegalovirus. | a sequence of 21,858 base pairs from the genome of human herpesvirus 6 (hhv-6) strain u1102 is presented. the sequence has a mean composition of 41% g + c, and the observed frequency of cpg dinucleotides is close to that predicted from this mononucleotide composition. the sequence contains 17 complete open reading frames (orfs) and part of another at the 5' end of the sequence. the predicted protein products of two of these orfs have no recognizable homologs in the genomes of other sequenced hum ... | 1990 | 2152817 |
| levels of epstein-barr virus dna in lymphoblastoid cell lines are correlated with frequencies of spontaneous lytic growth but not with levels of expression of ebna-1, ebna-2, or latent membrane protein. | the process of epstein-barr virus (ebv)-induced transformation of human b lymphocytes results in a cell line that is a mixture of latently and lytically infected cells, with the lytic cells composing roughly 5% to less than 0.0001% of the overall population. a set of nine normal lymphoblastoid cell lines that span a 100- to 200-fold range in average ebv dna content were studied, and the frequency with which these cells entered a lytic phase of viral growth correlated with their ebv dna copy numb ... | 1990 | 2152830 |
| functional human t cell-b cell hybridomas established from fusion of normal t cells and an ebv-transformed b cell line. | human t cell-b cell hybridomas containing 92 chromosomes were derived by fusing normal stimulated cd-depleted t cells with the ebv-transformed human b cell line 729-hf. these hybridomas coexpressed the t cell surface markers cd3, cd8, and cd2 and the b cell surface antigens cd19, cd20, cd23, dr, and dq. they weakly and variably expressed surface igm and tcr. genomic analysis revealed the presence of rearranged ig genes as well as beta-t cell receptor genes that could be ascribed to the b and t c ... | 1990 | 2152857 |
| clonal analysis of a human antibody response. quantitation of precursors of antibody-producing cells and generation and characterization of monoclonal igm, igg, and iga to rabies virus. | we quantitated and characterized the changes in the human b cell repertoire, at the clonal level, before and after immunization with rabies virus. moreover, we generated 10 monoclonal cell lines producing igm, igg, and iga antibodies to the virus. we found that in healthy subjects, not previously exposed to the virus, nearly 2% of the circulating b lymphocytes were committed to the production of antibodies that bound the virus. these b cells expressed the surface cd5 molecule. the antibodies the ... | 1990 | 2153188 |
| complement receptor type 1 (c3b/c4b receptor; cd35) and complement receptor type 2 (c3d/epstein-barr virus receptor; cd21). | 1990 | 2153507 | |
| establishment and characterization of two epithelial tumor cell lines (hne-1 and hone-1) latently infected with epstein-barr virus and derived from nasopharyngeal carcinomas. | two epithelial tumor cell lines were established from biopsy specimens of 2 nasopharyngeal carcinomas (npc) and designated hne-1 and hone-1. uncloned hne-1 cells were found to be epstein-barr virus (ebv) dna-positive when examined by southern blot analysis up to passage 35, after which the ebv genome could no longer be detected. a similar loss of ebv dna took place in uncloned hone-1 cells. however, hone-1 clone 40 cells are still ebv dna-positive up to passage 42 thus far and cell cultures cont ... | 1990 | 2153642 |
| oral hairy leukoplakia. | oral hairy leukoplakia was first described in homosexual men infected with the human immunodeficiency virus. it is thought to be caused by infection with both the epstein-barr virus and human papillomavirus. we report 59 cases of oral hairy leukoplakia. the disease was diagnosed in patients in all risk groups and was categorized in all classes of the walter reed classification without significant differences in prevalence. epstein-barr virus could be demonstrated in all tissue samples examined; ... | 1990 | 2153716 |
| encephalitis due to epstein-barr virus. | acute encephalitis due to epstein-barr virus is described. the initial diagnosis was based on clinical features and a positive monospot test. early treatment with intravenous acyclovir was given; steroids were not used. the patient made a rapid and complete recovery from a comatose state. the effectiveness of acyclovir in this condition remains unproven but early treatment is recommended in severely ill patients whose prognosis is uncertain. | 1990 | 2153730 |
| ebv ig-like domains. | 1990 | 2153934 | |
| isolation of a new herpesvirus from human cd4+ t cells. | a new human herpesvirus has been isolated from cd4+ t cells purified from peripheral blood mononuclear cells of a healthy individual (rk), following incubation of the cells under conditions promoting t-cell activation. the virus could not be recovered from nonactivated cells. cultures of lymphocytes infected with the rk virus exhibited a cytopathic effect, and electron microscopic analyses revealed a characteristic herpesvirus structure. rk virus dna did not hybridize with large probes derived f ... | 1990 | 2153965 |
| oligoclonal immunoglobulin heavy chain gene rearrangement in a childhood immunoblastic lymphoma. presentation as a polyphenotypic atypical lymphoproliferative reaction. | the authors describe a diagnostically difficult case of childhood lymphoma that presented as an atypical polyphenotypic lymphoproliferative reaction. initial immunophenotyping revealed the presence of igg, iga, kappa, and lambda within the neoplastic lymphocytes. the patient had circulating plasmacytoid lymphocytes and a polyclonal hypergammaglobulinemia. the patient died of widespread immunoblastic lymphoma in two months. postmortem tumor dna showed a oligoclonal pattern of immunoglobulin heavy ... | 1990 | 2154088 |
| serological diagnosis of infection with human herpesvirus type 6. | to identify clinical consequences of acute human herpesvirus type 6 infection by hypothesising that the virus will induce similar clinical syndromes to cytomegalovirus. | 1990 | 2154274 |
| regulation of c-fgr proto-oncogene expression in epstein-barr virus infected b-cell lines. | we and others have previously shown that in vitro conversion of epstein-barr virus (ebv)-negative burkitt lymphoma (bl) cell lines with the immortalizing b95-8 strain of ebv results in a marked elevation in levels of c-fgr proto-oncogene mrna. we now show, using a nuclear run-off assay, that this induction results from an increase in the rate of transcription of the c-fgr gene. we also show that bl cell lines freshly converted with the non-immortalizing hr-i strain of ebv do not accumulate highe ... | 1990 | 2154410 |
| the zta transactivator involved in induction of lytic cycle gene expression in epstein-barr virus-infected lymphocytes binds to both ap-1 and zre sites in target promoter and enhancer regions. | the bzlf1 or zta immediate-early gene of epstein-barr virus (ebv) encodes a 33-kilodalton phosphorylated nuclear protein that is a specific transcriptional activator of the ebv lytic cycle when introduced into latently infected b lymphocytes. we have shown previously that the divergent ebv dsl target promoter contains two zta-response regions, one within the minimal promoter and the other in an upstream lymphocyte-dependent enhancer region. in this study, we used footprinting and gel mobility re ... | 1990 | 2154599 |
| identification of phorbol ester response elements in the promoter of epstein-barr virus putative lytic switch gene bzlf1. | the product of the epstein-barr virus bzlf1 gene encodes a protein which is related to c-fos, it has been shown to bind specifically to a consensus ap-1 site, and its expression in latently epstein-barr virus-infected lymphocytes is sufficient to trigger the viral lytic cycle. we identified several elements within the bzlf1 promoter (zp) which are responsive to the phorbol ester 12-o-tetradecanoylphorbol-13-acetate (tpa), an inducer of the viral lytic cycle. these elements fall into two classes ... | 1990 | 2154605 |
| autoregulation of epstein-barr virus putative lytic switch gene bzlf1. | expression of the epstein-barr virus (ebv) bzlf1 gene in latently infected lymphocytes is sufficient to trigger the viral lytic cycle. as shown in the accompanying report (e. flemington and s.h. speck, j. virol. 64:1217-1226, 1990), the promoter for the bzlf1 gene (zp) contains two distinct types of elements (zi and zii [an ap-1-like domain]) which are responsive to the phorbol ester 12-o-tetradecanoylphorbol-13-acetate (tpa), an inducer of the viral lytic cycle. although zp can be activated wit ... | 1990 | 2154606 |
| epstein-barr virus internalization and infectivity are blocked by selective protein kinase c inhibitors. | selective protein kinase c inhibitors can either block or significantly reduce epstein-barr virus infectivity: inhibition of transformation and decreased 3h-thymidine (3h-tdr) incorporation in human b lymphocytes infected with b95-8 ebv, as well as a significant reduction in the induction of early antigens in raji cells superinfected by p3hri ebv was achieved by pre-treating the cells with the inhibitors. the inhibitors do not act by blocking binding of the virus to its cellular receptor cr 2, b ... | 1990 | 2155183 |
| deregulated c-myc expression in epstein-barr-virus-immortalized b-cells induces altered growth properties and surface phenotype but not tumorigenicity. | endemic burkitt's lymphoma (ebl) is characterized by the presence of epstein-barr virus (ebv) and a chromosomal translocation which results in deregulation and constitutive expression of the c-myc proto-oncogene. in order to examine the role played by activation of c-myc in determining the ebl phenotype, we have introduced into ebv-immortalized lymphoblastoid cells (lcl) plasmids which permit constitutive expression of c-myc. the resulting cells show a reduced serum dependence, reduced homotypic ... | 1990 | 2155186 |
| antibody patterns against cytomegalovirus and epstein-barr virus in human atherosclerosis. | the immune response against cytomegalovirus (cmv) and epstein-barr virus (ebv)-induced antigens has been determined in teh sera of 36 patients with angiographically assessed atherosclerosis and compared with that of 36 matched control subjects. significantly higher titres of antibody against cmv and ebv - induced antigens and a significant increase in cmv- and ebv- reactivated infections were found in atherosclerotic patients as compared to control subjects. moreover serological signs of concomi ... | 1990 | 2155375 |
| effect of cytokines on proliferation of epstein barr virus-transformed b lymphocytes. | plating efficiencies of ebv transformed human b cells seeded in single cell cultures are far lower (less than 1%) than observed in t cell cloning experiments. this report describes the stimulatory effect of several crude as well as recombinant growth factors on proliferation of ebv transformed b cells measured by [3h]thymidine uptake. supernatant of lps activated monocytes (hsf) and recombinant interleukin 4 (ril-4), but not recombinant il-1 beta, il-2, il-6, tnf alpha, gm-csf, and interferon ga ... | 1990 | 2155870 |
| unusual manifestations of epstein-barr virus encephalomyelitis. | a patient with atypical manifestations of epstein-barr virus (ebv) encephalomyelitis is presented. the patient had unusual spinal fluid immunoglobulin abnormalities, the syndrome of inappropriate anti-diuretic hormone secretion, autonomic dysfunction and spinal arachnoiditis. the cisternal csf, with a very high igg level (34.3 mg/dl; normal less than 6.1) but a normal albumin content, displayed evidence of massive intrathecal immunoglobulin production. this was further confirmed by the presence ... | 1990 | 2155877 |
| immortalization of epstein-barr virus-infected cd23-negative b lymphocytes by the addition of b cell growth factor. | epstein-barr (eb) virus-immortalized b lymphocytes coexpress the eb viral latent gene products (eb viral nuclear antigens 1 to 6, the latent membrane protein and the terminal protein gene products) and the cellular activation antigen cd23. immortalized b cells can be separated from those which are infected but not immortalized on the basis of cd23 expression as early as 2 days after in vitro infection. in the present report we have confirmed these data, but show that if left in culture for 7 day ... | 1990 | 2155999 |
| is epstein-barr virus involved in primary iga nephropathy? | 1990 | 2156182 | |
| identification and chromosomal mapping of new human tyrosine kinase genes. | to identify novel protein tyrosine kinase (ptk) genes expressed in human lymphoid cells, we have screened b- and t-cell cdna libraries at low stringency using a c-fms tyrosine kinase domain probe. three new ptk genes were identified, based on the presence of conserved amino acid sequence motifs characteristic of the catalytic domain of tyrosine kinases. of these three genes, one (tyk1) appears to be the human homologue of a previously cloned murine gene (ltk), which has been reported to encode a ... | 1990 | 2156206 |
| the detection of epstein-barr virus in hairy cell leukemia cells by in situ hybridization. | epstein-barr virus (ebv) has been implicated in the pathogenesis of several b-cell lymphoid proliferations. because patients with hairy cell leukemia (hcl) have a high incidence of seropositivity for ebv antigens, we studied the cells of hcl for evidence of ebv infection using in situ hybridization techniques. ebv mrna was detected in the tumor cells in four of six cases using a radiolabeled rna probe. confirmatory serologic data were available in three cases in which the viral dna was detected ... | 1990 | 2156435 |
| detection of epstein-barr virus in epidermal skin lesions of an immunocompromised patient. | using in-situ hybridizaiton, we showed the presence of the epstein-barr (eb) virus genome in epidermal cells from a patient with chronic lymphocytic leukemia and unusual cutaneous lesions characterized clinically by a maculopapular eruption and histologically by epidermal cell degeneration and lymphoid cell infiltration. such histologic changes are similar to those seen in graft-versus-host disease. the eb virus genome was mainly detected in the basal, germinative cells of the abnormal epitheliu ... | 1990 | 2156477 |
| characterization of three human monoclonal antibodies specific for polymorphic class i and class ii hla antigens. | three human monoclonal antibodies were derived from a single polytransfused patient awaiting renal transplantation. in microcitotoxicity assays, the patient's serum displayed strong positive reactions against greater than 90% of a panel of cells representing the known hla specificities. the donor's peripheral blood lymphocytes were infected with epstein-barr virus, cloned, and supernatants of the virus transformed cultures were screened for the presence of igg antilymphocyte reactivity utilizing ... | 1990 | 2156787 |
| intracellular trafficking of two major epstein-barr virus glycoproteins, gp350/220 and gp110. | the processing and intracellular localization of the two predominant epstein-barr virus glycoproteins expressed in late lytic infection were investigated. immune light or electron microscopy of frozen fixed sections revealed that gp110 colocalized to the endoplasmic reticulum and to the nuclear membrane with the endoplasmic reticulum-resident protein, heavy-chain-binding protein (bip), while gp350/220 accumulated in low abundance in the endoplasmic reticulum and was present in higher abundance i ... | 1990 | 2157039 |
| persistent nonproductive infection of epstein-barr virus-transformed human b lymphocytes by human immunodeficiency virus type 1. | we have studied the interaction of different strains of human immunodeficiency virus type 1 (hiv-1) with an epstein-barr virus-transformed human b-lymphocyte line, x50-7. previously we found that some hiv-1 strains replicated rapidly and were exclusively cytolytic; others induced persistent noncytopathic infection associated with continued shedding of extracellular virus (k. dahl, k. martin, and g. miller, j. virol. 61:1602-1608, 1987). we now describe a third form of cell-virus relationship in ... | 1990 | 2157058 |
| cdna cloning and transient expression of the epstein-barr virus-determined nuclear antigen ebna3b in human cells and identification of novel transcripts from its coding region. | recombinant plasmids containing sequences from the bamhi-e rightward reading frames 2a and 2b (berf2a and 2b) of the epstein-barr virus (ebv) genome were isolated from a library of cdna clones which had been previously made from the ebv b95-8 lymphoblastoid cell line (m. bodescot, o. brison, and m. perricaudet, nucleic acids res. 14:7103-7114, 1986). the characterization of these clones in combination with rnase mapping experiments led to the identification of one leftward and several rightward ... | 1990 | 2157061 |
| the epstein-barr virus brlf1 immediate-early gene product transactivates the human immunodeficiency virus type 1 long terminal repeat by a mechanism which is enhancer independent. | the epstein-barr virus (ebv) immediate-early gene product, brlf1, transactivates the human immunodeficiency virus type 1 (hiv-1) long terminal repeat. brlf1-induced transactivation of hiv-1 promoter constructs is accompanied by an increase in plasmid mrna and is reporter gene independent. previously, brlf1 transactivation of ebv promoters has been mapped to regions which function as enhancer elements. deletional analysis demonstrates that brlf1 transactivation of the hiv-1 promoter does not requ ... | 1990 | 2157062 |
| differences in human cell lines to support stable replication of epstein-barr virus-based vectors. | vectors carrying the origin of replication, ori-p, of the epstein-barr virus (ebv) are maintained extrachromosomally in human cells expressing the ebv nuclear antigen 1 (ebna-1). we have studied the ebv vectors p201 and p292 in which both ori-p and ebna-1 functions are present using the human cell lines a431 and hela. the two lines showed differences in their transfectability by the ebv vectors. thousands of hela transfectants were obtained with either vector and these remained intact as episome ... | 1990 | 2157493 |
| rotavirus induces proliferative response and augments non-specific cytotoxic activity of lymphocytes in humans. | in vitro cell-mediated immune responses to rotavirus in humans were studied. peripheral blood mononuclear cells (pbmc) of healthy adults proliferated in response to stimulation with the infectious and u.v.-inactivated wa strain of human rotavirus, showing a maximum response on day 7 of culture; however, cord blood lymphocytes failed to respond to rotavirus. a cross-reactive proliferative response of pbmc detected by stimulation with the ncdv strain of bovine rotavirus suggests the existence of e ... | 1990 | 2157566 |
| establishment of immortalized primate epithelial cells with sub-genomic ebv dna. | the genetic information in a sub-fragment of ebv dna, designated p31 (containing less than a quarter of the viral genome and derived from a recombinant dna cosmid library) allows epithelial cells from primary monkey and human kidney cultures to escape senescence under standard tissue culture conditions. a number of epithelial cell lines, designated m1/31, 483/31, 199/31 and hk/31, have been established and characterized following transfection of primary cells with p31 dna. they share many proper ... | 1990 | 2157679 |
| detection of epstein-barr virus dna in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe. | the presence of epstein barr virus (ebv) dna in biopsies from the post-nasal space (pns) of patients suspected of nasopharyngeal carcinoma (npc) was detected by in situ cytohybridization with an ebv dna probe labelled with the novel labelling compound digoxigenin. the digoxigenin probe was hybridised to cryostat sections of npc biopsies and subsequently detected by an enzyme immunoassay procedure. it was found that in situ cytohybridization using the digoxigenin probe was much more rapid and sen ... | 1990 | 2157729 |
| immunoradiometric assay for cytomegalovirus-specific igg antibodies: assay development and evaluation in blood transfusion practice. | an immunoradiometric assay (radio-immunosorbent test; rist) for the detection of igg antibodies to human herpesvirus 4 [human cytomegalovirus (cmv)] has been developed. the technique utilizes cmv antigen passively adsorbed to a polyvinyl microtitration plate and a radiolabelled murine monoclonal anti-human igg antibody to detect binding of human antibody to the 'solid phase' reagent. the assay was optimized, and its specificity confirmed by testing paired acute and convalescent sera from patient ... | 1990 | 2157732 |
| the switch between latency and replication of epstein-barr virus. | latency is characteristic behavior of many viruses that persist in their hosts. during latency an intact viral genome resides in the cell, but most viral genes are silent. diverse inducing stimuli, by which the environment interacts with the host cell, trigger the latent viral genome into replication. experiments are described that led to the identification of a crucial epstein-barr virus (ebv) gene, called zebra, which acts as a switch between latency and replication of this herpes virus. durin ... | 1990 | 2157769 |
| dual antibody rises to cytomegalovirus and human herpesvirus type 6: frequency of occurrence in cmv infections and evidence for genuine reactivity to both viruses. | the frequency of high (greater than 256) igg anti-human herpesvirus type 6 (hhv-6) titers in sera known to be positive for igm anti-cytomegalovirus (cmv) or igm anti-epstein barr virus (ebv) was significantly greater than in sera from healthy controls or from a group of ill patients who were cmv and ebv igm-negative (15/25 and 17/25 vs. 1/25 and 2/25, respectively, p less than .001). there was serologic evidence of simultaneous hhv-6 infection or reactivation (a rise in igg anti-hhv-6 titer or t ... | 1990 | 2157772 |
| epstein-barr virus nuclear antigen 2 induces expression of the virus-encoded latent membrane protein. | infection of epstein-barr virus-negative human b-lymphoma cell lines with the fully transforming b95.8 epstein-barr virus strain was associated with complete virus latent gene expression and a change in the cell surface and growth phenotype toward that of in vitro-transformed lymphoblastoid cell lines. in contrast, the cells infected with the p3hr1 epstein-barr virus strain, a deletion mutant that cannot encode epstein-barr nuclear antigen 2 (ebna2) or a full-length ebna-lp, expressed ebnas1, 3a ... | 1990 | 2157875 |
| epstein-barr virus latent membrane protein (lmp1) and nuclear proteins 2 and 3c are effectors of phenotypic changes in b lymphocytes: ebna-2 and lmp1 cooperatively induce cd23. | latent epstein-barr virus (ebv) infection and growth transformation of b lymphocytes is characterized by ebv nuclear and membrane protein expression (ebv nuclear antigen [ebna] and latent membrane protein [lmp], respectively). lmp1 is known to be an oncogene in rodent fibroblasts and to induce b-lymphocyte activation and cellular adhesion molecules in the ebv-negative burkitt's lymphoma cell line louckes. ebna-2 is required for ebv-induced growth transformation; it lowers rodent fibroblast serum ... | 1990 | 2157887 |
| [adcc against human class ii antigens in renal transplantation]. | antibody-dependent cell-mediated cytotoxicity (adcc) against epstein-barr virus induced human lymphoblastoid cell lines (lcl) in renal transplantation was examined. anti-dr sera were able to sensitize lcl corresponding dr antigens in adcc, while they were not able to sensitize pha induced lymphoblasts. the adcc activity against lcl was abolished by absorption of the dr antibodies. it was suggested that lcl were superior to pha induced lymphoblasts as targets for adcc. using lcl and anti-dr sera ... | 1990 | 2157948 |
| epstein-barr virus (ebv) receptors on epithelial hybrid cells derived from nasopharyngeal carcinoma. | we previously established an epstein-barr virus (ebv) genome-positive epithelial hybrid cell line, designated npc-kt, which was prepared by fusing primary epithelial cells derived from nasopharyngeal carcinoma (npc) with an epithelial ad-ah cell line. in this study, we measured the presence of the ebv receptors using radiolabelled ebv. it was determined that similar amounts of burkitt's lymphoma (bl)-derived p3hr-1 or npc-kt-derived ebv can attach bl-derived raji cells, but that only p3hr-1 viru ... | 1990 | 2158034 |
| human cytotoxic t-cell responses against epstein-barr virus nuclear antigens demonstrated by using recombinant vaccinia viruses. | the potentially pathogenic effects of infection with epstein-barr virus (ebv), a b-lymphotropic agent with cell growth-transforming potential, are contained in healthy virus carriers by virus-specific cytotoxic t-lymphocyte (ctl) surveillance. the target antigens against which such ctl responses are directed are yet undefined, but the antigens probably derived from one or more of the ebv "latent" proteins constitutively expressed in virus-transformed b cells. we have analyzed target specificity ... | 1990 | 2158098 |
| the b lymphocyte: a newly recognized source of reactive oxygen species with immunoregulatory potential. | 1990 | 2158475 | |
| the antibody repertoire of early human b cells. ii: expression of anti-dna-related idiotypes. | cord blood b cells were immortalized in vitro with epstein-barr virus (ebv). supernatants containing greater than 500 ng/ml igm from clones/lines were tested for expression of anti-dna-associated 16/6 and pr4 idiotypes (id) by elisa. four of 70 lines, but no clones, were positive for 16/6 id and none expressed the pr4 id. the presence of 16/6 id on four cell lines was associated with specificity for ssdna, cardiolipin and fc of igg. no association was seen with binding to the k30 polysaccharide ... | 1990 | 2158785 |
| [ataxia telangiectasia and characterization of its immunological disorders]. | ataxia telangiectasia (at) is a primary immunodeficiency syndrome characterized by cerebellar ataxia, extrapyramidal signs, oculocutaneous telangiectasia, recurrent respiratory infections and development of malignancies. at is a complex autosomal recessive disorder involving several systems other than lymphoid cells or the central nervous system. such a diversity of abnormalities includes hypersensitivity of fibroblasts and lymphocytes to ionizing radiation (anomaly of dna repair), non-random ch ... | 1990 | 2158803 |
| demonstration of epstein-barr virus in primary brain lymphoma by in situ dna hybridisation in paraffin wax embedded tissue. | tumour tissue from 29 patients with primary brain lymphoma was reviewed to determine if there was an aetiological association between epstein-barr virus and polyclonal and monoclonal lymphoproliferations. the morphology and immunophenotype in 24 patients for whom paraffin wax embedded tissue was available were studied. a high grade pleomorphic tumour morphology with plasmacytoid features was seen in 13 tumours. because of the large number of pleomorphic lymphomas, all tumours were examined for t ... | 1990 | 2159030 |
| complement receptor 2 mediates enhancement of human immunodeficiency virus 1 infection in epstein-barr virus-carrying b cells. | although the cd4 glycoprotein is the primary receptor for hiv-1, recent reports have suggested that other molecules might be involved in the enhancement of hiv-1 infection. we investigated the possible role of the complement receptor 2 in enhancement of hiv-1 infection in cd4+ ebv-containing b cells by infecting such cells in the presence of sera from hiv sero-positive donors, with or without added human complement. a marked increase in production of viral p24 and infectious progeny virus was ob ... | 1990 | 2159052 |
| [molecular analysis in argentinian patients with burkitt's lymphoma]. | burkitt's lymphoma (bl) is a b-cell neoplasm that occurs in children throughout the world. two distinct entities of this disease have been recognized: endemic bl (ebl), described in equatorial africa, and sporadic bl (sbl), described in the usa and europe. there are striking differences in the incidence, epidemiology, clinical presentation and molecular characteristics of the tumour cells between the two forms, although all bl carry identical specific chromosomal translocations. we have examined ... | 1990 | 2159189 |
| heterotransplantation of human lymphoid neoplasms using a nude mouse intraocular xenograft model. | there are few effective models for the study of human lymphoid neoplasms, including in vivo xenografts in immunocompromised animals. exploiting the additional immune privilege of the anterior chamber of the nude mouse eye, a novel method of direct heterotransplantation of cells from childhood leukemias and lymphomas has been developed. the establishment and characterization of 18 lymphoid xenograft cell lines maintained in the nude mouse intraocular model are reported. cell sources for heterotra ... | 1990 | 2159371 |
| the seroepidemiology of human herpesvirus-6 (hhv-6) from a case-control study of leukaemia and lymphoma. | sera from an epidemiological case-control study of leukaemias and lymphomas conducted between 1980 and 1986 were examined for reactivity to human herpesvirus-6 (hhv-6) by an indirect immunofluorescence assay. statistical analyses of the data revealed higher hhv-6 seroprevalence and antibody titres in the cases, particularly evident in the disease subtypes acute myeloid leukaemia, hodgkin's disease (hd), and low-grade non-hodgkin's lymphoma. within the control group alone, hhv-6 seroprevalence wa ... | 1990 | 2159435 |
| detection of a soluble form of the cd30 antigen in sera of patients with lymphoma, adult t-cell leukemia and infectious mononucleosis. | using a sandwich enzyme-linked immunosorbent assay (elisa) we were able to detect a soluble form of the cd30 antigen (cd30s) in the supernatant of cell lines expressing membrane-bound cd30 and in t and b cells after transformation with human t-cell leukemia virus (htlv-i) and epstein-barr-virus (ebv). while cd30s was not found in 250 healthy controls, it was detected in the sera of patients with hodgkin's disease (23/100), anaplastic large-cell (6/9), angioimmunoblastic (2/2) and one unclassifie ... | 1990 | 2159438 |
| in vitro transcriptional activation, dimerization, and dna-binding specificity of the epstein-barr virus zta protein. | the epstein-barr virus bzlf1 immediate-early gene encodes a transcriptional activator protein, zta, which acts as a key regulatory switch in the transition between the latent and lytic viral life cycle. in this work, full-length zta was expressed at high levels in escherichia coli and purified to homogeneity by dna affinity chromatography. the bacterial protein bound to specific target sequences (zta response elements) and activated transcription in vitro from an epstein-barr virus early target ... | 1990 | 2159531 |
| identification of interleukin-6 as an autocrine growth factor for epstein-barr virus-immortalized b cells. | autocrine growth factors are believed to be important for maintenance of an immortalized state by epstein-barr virus (ebv), because cell-free supernatants of ebv-immortalized cell lines promote the proliferation of autologous cells and permit their growth at low cell density. in this study, we provide evidence for the existence of two autocrine growth factor activities produced by ebv-immortalized lines distinguished by size and biological activities. much of the autocrine growth factor activity ... | 1990 | 2159561 |
| establishment and characterisation of an epstein-barr virus negative b immunoblastic lymphoma cell line. | 1990 | 2159767 | |
| establishment of a b-lymphoblastoid cell line infected with epstein-barr-related virus from a cynomolgus monkey (macaca fascicularis). | a b-lymphoblastoid cell line (ts-b) was established from lymph nodes of an apparently healthy cynomolgus monkey. the cells were demonstrated to contain epstein-barr virus-related antigens. moreover, herpesvirus particles were found in the cells by electron microscopy. the cell-free culture medium transformed lymphocytes of cynomolgus rhesus monkeys, and of japanese monkeys. | 1990 | 2160013 |
| characterisation of the ldl receptor in epstein-barr virus transformed lymphocytes. | non-dividing human lymphocytes were transformed upon infection with the epstein-barr virus (ebv) into lymphoblasts which are capable of continuous growth in culture. we studied the properties of the ldl receptor in ebv-transformed human lymphocytes (ebv-l) by binding experiments and by ligand blotting. ebv-l show a high affinity binding of ldl in the same order of magnitude as found with fibroblasts; ebv-l obtained from a homozygous familial hypercholesterolemic (fh) patient fail to express ldl ... | 1990 | 2160281 |
| role of 1q trisomy in tumorigenicity, growth, and metastasis of human leukemic b-cell clones in nude mice. | in order to investigate the association between various karyotypes of human tumor cells and biological behavior of tumors such as tumorigenicity, rate of growth, and the capacity to form metastasis, six chromosomally distinctive clones were isolated from an epstein-barr virus-transformed human chronic lymphocytic leukemic b-cell line which progressively grew and metastasized in irradiated nude mice. when inoculated into nude mice one clone (d10-1) with the karyotype of 46,xy,dup(1)(q11----q32) w ... | 1990 | 2160324 |
| papular and papulovesicular acrolocated eruptions and viral infections. | twenty-six children with a symmetric papular or papulovesicular acrolocated eruption were studied to evaluate a possible link with viral infection. a history of a recent immunization was present in four patients, two of whom had a polio vaccine virus isolated from their stool samples. there was serologic evidence of a recent epstein-barr virus infection in one patient, adenovirus infection in another, a concurrent epstein-barr virus and adenovirus infection in a third, and cytomegalovirus infect ... | 1990 | 2160656 |
| viral pneumonia in recipients of solid organ transplants. | viral pulmonary infections are a major cause of morbidity and mortality in solid organ transplant recipients. the herpes viruses-cytomegalovirus, herpes simplex virus, varicella zoster virus, and epstein-barr virus--cause most of the viral infections in this population. respiratory syncytial virus, adenovirus, and human immunodeficiency virus also cause pneumonitis in the transplant recipient. differences in the clinical and laboratory presentation of pneumonitis due to the various viral agents ... | 1990 | 2160718 |
| inhibition of specific binding of ebna 1 to dna by murine monoclonal and certain human polyclonal antibodies. | ebna 1 was expressed as a nonfusion protein in escherichia coli under control of the lac promoter. the major immunoreactive ebna 1 proteins migrated as two doublets with molecular masses of about 39/41 and 49/51 kda. gel mobility shift experiments showed that these products exhibit the sequence-specific dna binding on ori p previously characterized for a 28-kda lambda n-fusion protein encompassing the carboxy third of the ebna 1 protein. three monoclonal antibodies previously found to react with ... | 1990 | 2161154 |
| the spectrum of clinical and laboratory findings resulting from human herpesvirus-6 (hhv-6) in patients with mononucleosis-like illnesses not resulting from epstein-barr virus or cytomegalovirus. | recently, the morphologic, immunologic, and molecular makeup of a new virus designated human herpesvirus-6 (hhv-6) has been described. because cell cultures of hhv-6-infected mononuclear cells showed prominent lymphocytic changes, it could be anticipated that mononucleosis-like illnesses or lymphoproliferative disorders would turn out to be manifestations of active hhv-6 infection. in the present study, blood samples from 27 patients previously categorized as having non-epstein-barr virus (non-e ... | 1990 | 2161178 |
| monoclonal antibodies against epstein-barr virus transformed b lymphocytes from a cll patient. | immunization of balb/c mice with ebv-cll-1 cells, derived from epstein-barr virus transformed b lymphocytes from a chronic lymphocytic leukemia (cll) patient, yielded 2 monoclonal antibodies (igg1 kappa and igg2a kappa) against a membrane antigen on a subset of normal b lymphocytes and non-hodgkin's lymphomas. immunofluorescence revealed strong reactivity of the antibodies with ebv-cll-1 cells and with most lymphocytes in tonsil follicles, in the intestinal wall, around splenic arterioles and ne ... | 1990 | 2161403 |
| homology of cytokine synthesis inhibitory factor (il-10) to the epstein-barr virus gene bcrfi. | complementary dna clones encoding mouse cytokine synthesis inhibitory factor (csif; interleukin-10), which inhibits cytokine synthesis by th1 helper t cells, were isolated and expressed. the predicted protein sequence shows extensive homology with an uncharacterized open reading frame, bcrfi, in the epstein-barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host. | 1990 | 2161559 |
| multiple risk factors of nasopharyngeal carcinoma: epstein-barr virus, malarial infection, cigarette smoking and familial tendency. | a community-based case-control study was carried out to assess multiple risk factors and familial aggregation of nasopharyngeal carcinoma (npc). all of the 347 pathologically-confirmed npc new cases were serially recruited from the national taiwan university hospital, and healthy community controls one-to-one matched with cases on age, sex and residence were selected from household registration offices. npc risk factors were obtained from the study subjects through standardized interviews accord ... | 1990 | 2161639 |
| [detection of epstein-barr virus specific ige antibody]. | to confirm the existence and investigate the biological significance of ige virus-specific antibodies, we studied epstein-barr virus (ebv)-specific ige antibody by enzyme-linked immunosorbent assay with an anti human ige monoclonal antibody. we detected ebv-specific ige antibody in sera not only from patients with the ebv associated diseases of infectious mononucleosis and nasopharyngeal carcinoma, but also from patients with bronchial asthma, collagen disease and healthy volunteers. however, th ... | 1990 | 2161645 |
| cpg methylation of viral dna in ebv-associated tumours. | in ebv-immortalized lymphoblastoid cell lines (lcls) a small number of "latent" proteins are expressed. these are the ebv nuclear antigens, ebnas 1-6, and a latent membrane protein, lmp. we have investigated the expression of these proteins in a variety of ebv-associated tumours and cell lines. whereas transplant and b-cell lymphomas from cotton-top tamarins appear to express the full range of antigens found in lcls, we and others have found that in burkitt's lymphomas (bl) and a nasopharyngeal ... | 1990 | 2161800 |
| automation of limiting dilution cytotoxicity assays. | limiting dilution analysis is a valuable technique for the quantitation and clonal analysis of immunocompetent cells. however, manual processing of the large number of samples necessary for satisfactory statistical analysis is laborious, and consequently results in inaccuracies reflected as increased standard errors. we describe the application of an automated robotic liquid handling tool to process samples in limiting dilution cytotoxicity assays. our studies have shown that automated liquid ha ... | 1990 | 2161883 |
| murine herpesvirus 68 is genetically related to the gammaherpesviruses epstein-barr virus and herpesvirus saimiri. | short nucleotide sequence analysis of seven restriction fragments of murine herpesvirus 68 (mhv-68) dna has been undertaken and used to determine the overall genome organization and relatedness of this virus to other well characterized representatives of the alpha-, beta- and gammaherpesvirus subgroups. nine genes have been identified which encode amino acid sequences with greater similarity to proteins of the gammaherpesvirus epstein-barr virus (ebv) than to the homologous products of the alpha ... | 1990 | 2161903 |
| epstein-barr virus-immortalized b cells produce il-6 as an autocrine growth factor. | the continuous proliferation of epstein-barr virus (ebv)-immortalized b cells is enhanced by autocrine as well as paracrine growth factors. in the present study, the possibility that ebv-immortalized b cells might produce interleukin-6 (il-6) proteins in an autocrine manner was examined. it was found that culture supernatants from ebv-transformed b cells, but not from burkitt's lymphoma lines, augmented the proliferation of an il-6-dependent murine hybridoma clone, mh60.bsf2. this growth-promoti ... | 1990 | 2162322 |
| accessory signals in t-t cell interactions between antigen- and alloantigen-specific, human memory t cells generated in vitro. | the potential of activated hla class ii-positive t cells as antigen-/alloantigen-presenting cells remains controversial. in our model system we use in vitro-primed, hla class ii-specific t cells of the memory t-cell phenotype, cd4+, cd29+ (4b4+), and cd45ro+ (uchl-1). we have previously shown that alloactivated, hla class ii-positive t cells (ta) are unable to stimulate proliferative responses in naive and primed allospecific t cells when 'back-stimulation' is avoided. the explanation of this fe ... | 1990 | 2162555 |
| viral antibodies in multiple sclerosis. a nationwide co-twin study. | serum viral antibody titers against 21 viruses were studied in 19 of 23 same-sex twin pairs with multiple sclerosis derived from the finnish twin cohort. thorough neurologic examinations showed two monozygotic pairs to be concordant, whereas all dizygotic pairs were discordant. special attention was given to measles, mumps, and rubella viruses, against which the antibody levels were determined with the complement fixation, hemagglutination inhibition, hemolysis-ingel, and enzyme immunoassay meth ... | 1990 | 2162658 |
| cyclosporin abrogates virus-specific t-cell control of ebv-induced b-cell lymphoproliferation. | renal and cardiac recipients undergoing aggressive immunosuppressive therapy with cyclosporin a have been reported to have unusually high incidences of epstein-barr virus (ebv) genome-positive lymphomas. t cells have been shown to be of critical importance in controlling the lymphoproliferative potential of the eb virus. ebv-specific t cell clones were generated in vitro by repeated antigenic restimulation in the presence of interleukin-2 (il-2). these virus-specific cells were used to study cyc ... | 1990 | 2163257 |
| lymphoepithelial carcinoma of the stomach with epstein-barr virus demonstrated by polymerase chain reaction. | epstein-barr virus dna was detected by polymerase chain reaction in a paraffin-embedded block of an undifferentiated lymphoepithelial gastric carcinoma but was absent in a poorly differentiated gastric adenocarcinoma which also had pronounced lymphoid stroma. this is the first report, to our knowledge, of a lymphoepithelial carcinoma of the stomach. the association with epstein-barr virus and the distinctive histologic appearance justify the separate classification of undifferentiated lymphoepit ... | 1990 | 2163534 |
| detection of epstein-barr virus genome in benign polyclonal proliferative t cells of a young male patient. | epstein-barr virus (ebv) dna was detected in polyclonal t cells that proliferated transiently in a 21-year-old male (referred to as h.j.) who underwent an apparently benign lymphocytosis (white blood cells, 31 x 10(6)/microl; lymphocyte, 79%) with fever, tonsillar swelling, lymphadenopathy, and hepatosplenomegaly. the symptoms and signs subsided mostly within a month of hospitalization. the major population of the lymphocytes at admission was positive for cd3, cd8 (4/8 ratio, 0.16), wt31, and dr ... | 1990 | 2163693 |
| [a senile case of b-cell lymphoma with high titers of an eb virus-associated antibodies]. | an 83-year-old man suffering from general malaise and discomfort in the epigastrium was admitted to hospital. on testing, peripheral blood and bone marrow smears disclosed a number of abnormal lymphocytoid cells, and a ct and a linear echo scan revealed splenomegaly and a multiple intraperitoneal lymphadenopathy, although no superficial lymph nodes were palpable. further, abnormal lymphocytoid cells were positive for b1, b4, okia1, leu1, tac, anti-igm, anti-igd, and anti-lambda. thus, a diagnosi ... | 1990 | 2164114 |