Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| distinct regulation of h2-m3-restricted memory t cell responses in lymph node and spleen. | cd8 t cell populations restricted by h2-m3 mhc class ib molecules expand rapidly during primary listeria monocytogenes infection but only minimally upon reinfection. in contrast, cd8 t cells restricted by mhc class ia molecules undergo more delayed expansion during primary infection but rapid and robust expansion following reinfection. in this study we demonstrate that primary h2-m3-restricted cd8 t cell responses are unaffected by the frequency of naive mhc class ia-restricted t cells during l. ... | 2005 | 16237094 |
| osteopontin is not required for the development of th1 responses and viral immunity. | osteopontin (opn) has been defined as a key cytokine promoting the release of il-12 and hence inducing the development of protective cell-mediated immunity to viruses and intracellular pathogens. to further characterize the role of opn in antiviral immunity, opn-deficient (opn-/-) mice were analyzed after infection with influenza virus and vaccinia virus. surprisingly, we found that viral clearance, lung inflammation, and recruitment of effector t cells to the lung were unaffected in opn-/- mice ... | 2005 | 16237095 |
| the importance of toll-like receptor 2 polymorphisms in severe infections. | toll-like receptor 2 (tlr2) is a member of the tlr family, which plays a central role in the innate immune response to a wide variety of microorganisms. animal studies have shown that tlr2-knockout mice are more susceptible to septicemia due to staphylococcus aureus and listeria monocytogenes, meningitis due to streptococcus pneumoniae, and infection with mycobacterium tuberculosis, suggesting that functional tlr2 polymorphisms may impair host response to a certain spectrum of microbial pathogen ... | 2005 | 16237639 |
| growth rate and growth probability of listeria monocytogenes in dairy, meat and seafood products in suboptimal conditions. | to evaluate the performances of models predicting the growth rate or the growth probability of listeria monocytogenes in food. | 2005 | 16238733 |
| the autolytic phenotype of the bacillus cereus group. | to determine the autolytic phenotype of five species in the bacillus cereus group. | 2005 | 16238737 |
| an investigation of the thermal inactivation of staphylococcus aureus and the potential for increased thermotolerance as a result of chilled storage. | the aims of this study were; (i) to provide thermal inactivation data for staphylococcus aureus; (ii) to examine the kinetics, including decimal reduction times (d-value) and rate constants (k), that describe the thermal inactivation of staph. aureus and to compare two different methods of calculating d-values and (iii) to determine whether or not chilled storage would toughen these microorganisms resulting in increased thermotolerance. | 2005 | 16238754 |
| gene silencing and overexpression of porcine peptidoglycan recognition protein long isoforms: involvement in beta-defensin-1 expression. | peptidoglycan recognition proteins (pgrps) are a group of newly identified proteins with emerging functions in mammalian innate immunity. here we report the identification and characterization of two long isoforms of porcine pgrp. their complete cdna sequences encode predicted peptides of 252 and 598 residues and are named ppgrp-l1 and ppgrp-l2, respectively. these porcine isoforms share identical pgrp domains at their c terminus, which are highly conserved with human and mouse orthologs. ppgrp- ... | 2005 | 16239507 |
| a prfa transposon mutant of listeria monocytogenes f2365, a serotype 4b strain, is able to survive in the gastrointestinal tract but does not cause systemic infection of the spleens and livers of intragastrically inoculated mice. | prfa is a member of the crp/fnr family of global regulatory genes in listeria monocytogenes that has been shown previously to regulate several key virulence determinants both in vitro and in parenterally inoculated laboratory rodents. however, the role of prfa in the ability of l. monocytogenes to cause infection via the gastrointestinal (gi) tract has not been clearly established. in this study, we used a prfa transposon mutant of l. monocytogenes f2365, a serotype 4b strain, to assess the role ... | 2005 | 16239554 |
| typing of food-borne listeria monocytogenes by polymerase chain reaction-restriction enzyme analysis and amplified fragment length polymorphism. | the applicability of polymerase chain reaction-restriction enzyme analysis (pcr-rea) and amplified fragment length polymorphism (aflp) for typing of food borne listeria monocytogenes strains was tested. a panel of 43 l. monocytogenes strains isolated from food, mostly serovars 1/2b or 1/2a, were analysed by the optimized pcr-rea oriented to inla and inlb genes and by aflp. by pcr-rea, five types of profiles were obtained. by aflp, the strains were separated into 11 types and 18 subtypes forming ... | 2005 | 16240700 |
| purification and characterization of curvaticin l442, a bacteriocin produced by lactobacillus curvatus l442. | lactobacillus curvatus l442, isolated from greek traditional fermented sausage prepared without the addition of starters, produces a bacteriocin, curvaticin l442, which is active against the pathogen listeria monocytogenes. the bacteriocin was purified by 50% ammonium sulphate precipitation, cation exchange, reverse phase and gel filtration chromatography. partial n-terminal sequence analysis using edman degradation revealed 30 amino acid residues, revealing high homology with the amino acid seq ... | 2006 | 16244793 |
| the food safety management system. | a comprehensive food safety strategy involves establishing risk management goals, food safety objectives and, for production systems, performance objectives and performance criteria. the working instructions for each step of the process should be validated for their effect before integration within a specific haccp plan. the importance of realistic inactivation models to predict the hygienic equivalence of food processing operations is discussed. | 2005 | 16244936 |
| molecular characterization of listeria monocytogenes strains associated with outbreaks of listeriosis in humans and ruminants and food products by serotyping and automated ribotyping. | 2005 | 16244967 | |
| antimicrobial effect of thai spices against listeria monocytogenes and salmonella typhimurium dt104. | the objective of this study was to determine the potential antimicrobial activity of extracts and essential oils of spices from thailand against foodborne pathogenic bacteria. the antimicrobial efficacy of ginger (zingiber officinale), fingerroot (boesenbergia pandurata), and turmeric (curcuma longa) was evaluated against five strains of listeria monocytogenes and four strains of salmonella enterica ssp. enterica serovar typhimurium dt104. antimicrobial activity was investigated in microbiologic ... | 2005 | 16245707 |
| evaluation of small-scale hot-water postpackaging pasteurization treatments for destruction of listeria monocytogenes on ready-to-eat beef snack sticks and natural-casing wieners. | this study was conducted to evaluate small-scale hot-water postpackaging pasteurization (ppp) as a postlethality (post-cooking) treatment for listeria monocytogenes on ready-to-eat beef snack sticks and natural-casing wieners. using a commercially available plastic packaging film specifically designed for ppp applications and 2.8 liters of boiling water (100 degrees c) in a sauce pan on a hot plate, an average reduction in l. monocytogenes numbers of > or = 2 log units was obtained using heating ... | 2005 | 16245708 |
| antilisterial activity of a carnobacterium piscicola isolated from brazilian smoked fish (surubim [pseudoplatystoma sp.]) and its activity against a persistent strain of listeria monocytogenes isolated from surubim. | data on the prevalence and growth of listeria monocytogenes in lightly preserved fish products from subtropical and tropical regions are very scarce. our research describes l. monocytogenes that was detected in 5% of the packages of cold-smoked surubim, a native brazilian freshwater fish that we analyzed, and shows that the strains isolated were of the same random amplified polymorphic dna subtype as the strains that were isolated from the same factory 4 years earlier. a bacteriocinogenic strain ... | 2005 | 16245709 |
| stability of enterocin as-48 in fruit and vegetable juices. | enterocin as-48 is a candidate bacteriocin for food biopreservation. before addressing application of as-48 to vegetable-based foods, the interaction between as-48 and vegetable food components and the stability of as-48 were studied. enterocin as-48 had variable interactions with fruit and vegetable juices, with complete, partial, or negligible loss of activity. for some juices, loss of activity was ameliorated by increasing the bacteriocin concentration, diluting the juice, or applying a heat ... | 2005 | 16245711 |
| efficacy of uv light for the reduction of listeria monocytogenes in goat's milk. | certain types of goat's cheeses are produced using unpasteurized milk, which increases the food safety concerns for these types of products. popularity and consumption of goat's milk products have increased, and the niche market includes gourmet goat's cheeses. the u.s. code of federal regulations and the pasteurized milk ordinance both address the possibility for processing alternatives to heat treatment, and the use of uv light treatment may be a viable alternative that still ensures the safet ... | 2005 | 16245732 |
| [effect of high hydrostatic pressure on microbial physiological characteristics]. | physiological characterizations of listeria monocytogenes nctc 11994 and escherichia coli atcc 80739 have deeply changed by high hydrostatic pressure. the results showed that counts of both microbial strains decreased 7 log cfu at 400mpa, 10 min. pressure treatments also resulted in change of intracellular ph value, lowed membrane potential, have internal potassium filtered out, and decreased atp concentration. | 2005 | 16245862 |
| identification of substrates of the listeria monocytogenes sortases a and b by a non-gel proteomic analysis. | sortases are enzymes that anchor surface proteins to the cell wall of gram-positive bacteria by cleaving a sorting motif located in the c-terminus of the protein substrate. the best-characterized motif is lpxtg, which is cleaved between the t and g residues. in this study, a non-gel proteomic approach was used to identify surface proteins recognized by the two sortases of listeria monocytogenes, srta and srtb. material containing peptidoglycan and strongly associated proteins was purified from s ... | 2005 | 16247833 |
| listeria-based vaccines for cancer treatment. | listeria monocytogenes is a facultative intracellular bacterium that enters professional antigen-presenting cells by active phagocytosis. as a live bacterium, it induces antigen-presenting cell maturation and strong innate immunity which may assist in the immune response to poorly immunogenic antigens, such as tumor-associated antigens. listeria produces virulence factors that allow it to escape from the phagolysosome and colonize the cytosol of the host cell. it is thus a potent vaccine vector ... | 2005 | 16248280 |
| comparison of genomic structures in the serovar 1/2a listeria monocytogenes isolated from meats and listeriosis patients in japan. | foodborne disease by listeria monocytogenes, serovar 1/2a has recently been reported in many countries. although contamination by this bacteria is also known to be gradually spreading among the marketed foods of japan, there is little information on relation between listeriosis and food contamination. in the present study, the characteristics of the genomic structures of serovar 1/2a were compared among the isolates from marketed meats and listeriosis patients. several isolates from meats purcha ... | 2005 | 16249623 |
| analysis of the molecular evolution of listeria monocytogenes isolated from japanese meats and environment. | food contaminated by listeria monocytogenes is a problem on a worldwide level because it is a serious food-borne pathogen. although 3 evolutionary divisions have been reported for l. monocytogenes, the evolution of japanese isolates has not yet been clarified. thus, in order to determine the lineage of these japanese isolates, we classified and conducted phylogenetic analysis of 407 bp (position 1116-1522) of the iap gene derived from 88 isolates from japanese listeriosis patients, foods and env ... | 2005 | 16249630 |
| adhesion controls bacterial actin polymerization-based movement. | as part of its infectious life cycle, the bacterial pathogen listeria monocytogenes propels itself through the host-cell cytoplasm by triggering the polymerization of host-cell actin near the bacterial surface, harnessing the activity of several cytoskeletal proteins used during actin-based cell crawling. to distinguish among several classes of biophysical models of actin-based bacterial movement, we used a high-throughput tracking technique to record the movement of many individual bacteria dur ... | 2005 | 16251274 |
| roles of group iia phospholipase a2 and complement in killing of bacteria by acute phase serum. | the complement system is regarded as an important component of the innate defence system against invading bacteria. however, synergistic actions between the complement and the other components of innate immunity are incompletely known. human group iia phospholipase a(2) (hgiia pla(2)) is an effective antibacterial enzyme in serum of patients with severe bacterial infections. our aim was to investigate the significance of complement and hgiia pla(2) in acute phase serum. serum samples were collec ... | 2005 | 16253130 |
| arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function. | rna interference silencing of up to 90% of arp3 protein expression, a major subunit of the arp2/3 complex, proportionately decreases the intracellular motility of listeria monocytogenes and actin nucleation activity ascribable to the arp2/3 complex in mouse embryonic fibroblasts. however, the arp2/3-deficient cells exhibit unimpaired lamellipodial actin network structure, translational locomotion, spreading, actin assembly, and ruffling responses. in addition, arp3-silenced cells expressing neur ... | 2005 | 16254049 |
| real-time polymerase chain reaction for detecting bacterial dna directly from blood of neonates being evaluated for sepsis. | speed is of the essence when evaluating an infant with symptoms consistent with sepsis. because of the high morbidity and mortality associated with neonatal sepsis, infants receive multiple, broad-spectrum antibiotics before receiving finalized blood culture results. incorporating an additional, reliable, yet rapid assay to detect bacteria directly from blood would facilitate timely diagnosis and appropriate care. to this end, we designed a real-time polymerase chain reaction (pcr) assay targeti ... | 2005 | 16258155 |
| prolonged dysphagia due to listeria-rhombencephalitis with brainstem abscess and acute polyradiculoneuritis. | we report a case of previously healthy student with acute rhombencephalitis and brainstem abscess caused by listeria monocytogenes. the disease begun with uncharacteristic prodromal symptoms of gastrointestinal infection followed by headache and vertigo. after hospital admission the patient rapidly deteriorated, presenting pronounced dysphagia and respiratory failure requiring mechanical ventilation. the diagnosis was established upon clinical symptoms of infection, brainstem involvement, typica ... | 2006 | 16260041 |
| [spontaneous bacterial peritonitis by listeria monocytogenes in a patient with liver cirrhosis]. | 2005 | 16266228 | |
| listeria monocytogenes meningitis following imatinib mesylate-induced monocytopenia in a patient with chronic myeloid leukemia. | 2005 | 16267747 | |
| application of measurements of transepithelial electrical resistance of intestinal epithelial cell monolayers to evaluate probiotic activity. | among five potentially probiotic lactobacilli investigated, lactobacillus plantarum mf1298 and lactobacillus salivarius dc5 showed the highest increase in the transepithelial electrical resistance (ter) of polarized monolayers of caco-2 cells, and this increase was shown to be dose dependent. furthermore, preincubation with mf1298 attenuated a decrease in ter induced by listeria monocytogenes. | 2005 | 16269795 |
| occurrence of listeria spp. in effluents of french urban wastewater treatment plants. | listeria spp. were found in most treated waters (84.4%) and raw sludge (89.2%) of six french urban wastewater treatment plants and one composting facility, examined monthly over a 1-year period. most strains belonged to listeria monocytogenes, serotypes 4b/4e being predominant. sludge composting and liming reduced or prevented listeria contamination. | 2005 | 16269804 |
| factors influencing the ability of listeria monocytogenes to pass through a membrane filter by active infiltration. | listeria monocytogenes infiltrated the reticulate structure of a membrane filter and passed through a filter with pore sizes of 0.45 microm and 0.2 microm in 6 to 24 h and 5 to 6 days, respectively. flagellar motility and expansive pressure generated by the growing bacterial population were indicated as the driving forces of infiltration. | 2005 | 16269806 |
| thymus-dependent memory phenotype cd8 t cells in naive b6.h-2kb-/-db-/- animals mediate an antigen-specific response against listeria monocytogenes. | b6.h-2kb-/-db-/- (dko) mice have greatly reduced numbers of mature cd8alphabeta t cells in their periphery. however, these non-class ia-selected cd8alphabeta t cells are able to mediate immune responses to a number of pathogens. approximately 60% of the cd8alphabeta t cells in the spleen and peripheral lymph nodes of naive dko mice display a memory (cd44high) phenotype. to investigate the origins of these non-class ia-selected cd8alphabetacd44high cells, we traced the phenotype of recent thymic ... | 2005 | 16272298 |
| regulation of expression of type i signal peptidases in listeria monocytogenes. | the role of type i signal peptidases (spases i) is to remove the signal peptides of preproteins exported by the general secretory pathway. the genome of listeria monocytogenes contains a locus encoding three contiguous spases i (denoted sipx, sipy and sipz). the authors recently showed that sipx and sipz perform distinct functions in protein secretion and bacterial pathogenicity. here, the regulation of sip gene expression in broth and in infected eukaryotic cells was studied. the results show t ... | 2005 | 16272398 |
| a systematic approach to determine global thermal inactivation parameters for various food pathogens. | thermal inactivation of pathogens has been studied extensively, which has resulted in a wide range of d- and z-values. estimating the inactivation rate for a specific condition based on these reported values is difficult, since one has to select representative conditions, and data obtained exactly at the required representative conditions are generally not available. therefore, a first step could be to globally assess a heat treatment taking into account largest effects only. once the most impor ... | 2006 | 16274824 |
| light enhances the bactericidal activity of human monocytes and neutrophils via hvem. | human monocytes and neutrophils play major roles in clearing bacteria from human blood and tissues. we found that the herpes virus entry mediator (hvem) was highly expressed in monocytes and neutrophils, and its interaction with "homologous to lymphotoxins, shows inducible expression, and competes with herpes simplex virus glycoprotein d for hvem/tumor necrosis factor (tnf)-related 2" (light) enhanced bactericidal activity against listeria monocytogenes and staphylococcus aureus. the light-hvem ... | 2006 | 16275888 |
| enzyme-electropolymer-based amperometric biosensors: an innovative platform for time-temperature integrators. | a novel exogenous time-temperature integrator (tti) based on an amperometric glucose oxidase biosensor is presented. the tti consists of the enzyme entrapped within an electrochemically generated poly(o-phenylenediamine) (popd) thin film deposited on the interior wall of a platinum (pt) or a platinized stainless steel (pt-ss) capsule. after thermal treatment, the tti is mounted in a continuous flow system and connected to a potentiostat for amperometric detection of residual enzyme activity. a m ... | 2005 | 16277377 |
| detection and characterization of tet(m) in tetracycline-resistant listeria strains from human and food-processing origins in belgium and france. | in the present study, three listeria monocytogenes strains and one listeria innocua strain out of a collection of 241 listeria isolates from human and food-processing sources were found to display resistance to tetracycline (tc) due to the presence of the tet(m) gene. through sequence analysis, it was shown that tet(m) genes in two of the isolates belong to sequence homology group (shg) ii, a group comprising chromosomally encoded tet(m) genes previously found in staphylococcus aureus and in lac ... | 2005 | 16278428 |
| surveillance of listeria infections in europe. | in addition to the economic consequences and threats associated with outbreaks, listeriosis remains of great public health concern, as it has one of the highest case fatality rates of all the foodborne infections (20%-30%), and has common source epidemic potential. changes in the way food is produced, distributed and stored have created the potential for diffuse and widespread outbreaks involving many countries. in 2002, a survey was carried out to assess the need for and the feasibility of a eu ... | 2005 | 16282642 |
| cellular lipid fatty acid pattern heterogeneity between reference and recent food isolates of listeria monocytogenes as a response to cold stress. | cells of four reference strains (scott a, lo 28, cnl 895807 and atcc 19115) and of five recent food isolates (a00m011, a00m018, a00m087, a00m092 and a00m123) of listeria monocytogenes were grown until late exponential phase in brain heart broth at two different temperatures (37 degrees c and 4 degrees c). our results show that significant differences exist between the cellular lipid fatty acid profile of reference and recent food isolates. like the reference strains, and in keeping with previous ... | 2005 | 16284926 |
| [focal peritonitis as a form of clinical presentation of listeriosis]. | infections by germs of listeria genus can occur sporadically or in epidemic outbreaks and have different clinical presentations. abdominal origin is very unusual among them, especially in no immunocompromised patients or without concurrent liver illness. we present a young healthy man who is lacking in underlying diseases, suffering from focal peritonitis caused by listeria monocytogenes. the patient had to be operated on, requiring immediate colectomy and evacuation of a retroperitoneal abscess ... | 2005 | 16288580 |
| quantification of listeria monocytogenes in salads by real time quantitative pcr. | a real time quantitative pcr (rtq-pcr) was carried out purifying dna extracts of listeria monocytogenes using a high pure listeria sample preparation kit and quantifying in a lightcycler system with hybridisation probes. a standard curve was constructed with serial dilutions. a range linear relationship, from 10 to 10(5)l. monocytogenes colony forming units (cfu), was observed between threshold cycle (ct) and logarithmic concentration of the serial dilutions. the assay was linear in a range from ... | 2006 | 16289408 |
| multiple synergizing factors contribute to the strength of the cd8+ t cell response against listeriolysin o. | immunodominance in cd8+ t cell responses against listeria monocytogenes is a well-recognized but still not fully understood phenomenon. from listeriolysin, the major virulence factor of l. monocytogenes, only a single epitope, pllo91-99, is presented by mhc class i molecules in balb/c mice which dominates the cytotoxic t cell response against this bacterial pathogen. to obtain more insights into the molecular and cellular mechanisms underlying immunodominance of this particular epitope, we compa ... | 2006 | 16291651 |
| a study of the role of multiple site blood cultures in the evaluation of neonatal sepsis. | the optimal number of blood cultures needed to document sepsis in an ill neonate has undergone little critical evaluation. multiple site cultures may improve pathogen detection if intermittent bacteremia occurs, or if a low density of bacteria is present in the blood. we hypothesized, however, that bacterial clearance is slower and bacteremia more continuous in septic neonates, so that a single site blood culture should be sufficient to accurately document true septicemia.objective:to determine ... | 2006 | 16292335 |
| influence of kinetic parameters of high pressure processing on bacterial inactivation in a buffer system. | high pressure processing is recently applied in the food industry to inactivate spoilage and pathogenic microorganisms. bacterial cells exhibit various barosensibility, and the role of pressurization, depressurization and constant pressure stage remain unknown. we investigated the effect of high pressure processing on salmonella typhimurium and listeria monocytogenes cells at 400 and 500 mpa respectively in buffer ph 7 at 20 degrees c. we applied various pressurization/depressurization kinetic r ... | 2006 | 16293331 |
| stochastically modeling listeria monocytogenes growth in farm tank milk. | this article presents a listeria monocytogenes growth model in milk at the farm bulk tank stage. the main objective was to judge the feasibility and value to risk assessors of introducing a complex model, including a complete thermal model, within a microbial quantitative risk assessment scheme. predictive microbiology models are used under varying temperature conditions to predict bacterial growth. input distributions are estimated based on data in the literature, when it is available. if not, ... | 2005 | 16297223 |
| loading history determines the velocity of actin-network growth. | directional polymerization of actin filaments in branched networks is one of the most powerful force-generating systems in eukaryotic cells. growth of densely cross-linked actin networks drives cell crawling, intracellular transport of vesicles and organelles, and movement of intracellular pathogens such as listeria monocytogenes. using a modified atomic force microscope (afm), we obtained force-velocity (fv) measurements of growing actin networks in vitro until network elongation ceased at the ... | 2005 | 16299496 |
| neurological complications in kidney transplant recipients. | neurological complications are frequent in renal transplant recipients and may largely contribute to morbidity and mortality. the postransplant neurological complications may be categorized into five areas: 1) immunosuppressive medications, 2) stroke, 3) peripheral neuropathies, 4) infection, and 5) malignancies. a number of complications are directly caused by the neurotoxicity of immunosuppressive agents. calcineurin-inhibitors may cause mild symptoms, such as tremors and paresthesia, or sever ... | 2005 | 16299677 |
| use of oligonucleotide array for identification of six foodborne pathogens and pseudomonas aeruginosa grown on selective media. | identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. a molecular identification method was developed in which universal primers were used to amplify the 16s to 23s rdna intergenic spacer of target microorganisms, and pcr products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. the seven target microorganisms we ... | 2005 | 16300063 |
| quantifying the robustness of a broth-based model for predicting listeria monocytogenes growth in meat and poultry products. | given the importance of listeria monocytogenes as a risk factor in meat and poultry products, there is a need to evaluate the relative robustness of predictive growth models applied to meat products. the u.s. department of agriculture-agricultural research service pathogen modeling program is a tool widely used by the food industry to estimate pathogen growth, survival, and inactivation in food. however, the robustness of the pathogen modeling program broth-based l. monocytogenes growth model in ... | 2005 | 16300067 |
| listeria monocytogenes inhibition by whey protein films and coatings incorporating lysozyme. | the effects of whey protein isolate (wpi) films and coatings incorporating lysozyme (lz) on the inhibition of listeria monocytogenes both in and on microbial media, as well as on cold-smoked salmon, were studied. the antimicrobial effects of lz were examined using various growth media by turbidity and plate counting tests. disc-covering and disc-surface-spreading tests were also used to evaluate the effects of wpi films incorporating lz. smoked salmon was used as a model food to test the antimic ... | 2005 | 16300068 |
| application of predictive models to estimate listeria monocytogenes growth on frankfurters treated with organic acid salts. | organic acid salts including sodium lactate, sodium diacetate, potassium benzoate, potassium sorbate, and their combinations were assessed as potential inhibitors of listeria monocytogenes growth on frankfurters. predictive models for l. monocytogenes growth on frankfurters treated with these salts were compared to select a proper l. monocytogenes growth curve model under these conditions. sigmoidal equations, including logistic and gompertz equations, are widely used to describe bacterial growt ... | 2005 | 16300069 |
| power ultrasound treatment of listeria monocytogenes in apple cider. | inactivation experiments with listeria monocytogenes 10403s, an ultrasound-resistant strain, were conducted at sublethal (20, 30, and 40 degrees c) and lethal (50, 55, and 60 degrees c) temperatures in saline solution (ph 7.0), acidified saline solution (ph 3.4), and apple cider (ph 3.4) with and without application of ultrasound (20 khz, 457 mw.ml(-l)). the survival of recoverable l. monocytogenes 10403s in apple cider was evaluated, and the effects of temperature, ultrasound, ph, and food matr ... | 2005 | 16300070 |
| starter cultures and high-pressure processing to improve the hygiene and safety of slightly fermented sausages. | the effectiveness of selected starter cultures and high hydrostatic pressure after ripening was evaluated to improve the safety and quality of slightly fermented sausages. inhibition of common foodborne pathogens, spoilage bacteria, and biogenic amine content was studied. random amplification of polymorphic dna and plasmid profiles were used to monitor the competitiveness of the starter cultures during fermentation and ripening. lactobacillus sakei ctc6626 and staphylococcus xylosus ctc6013 domi ... | 2005 | 16300071 |
| efficacy of cetylpyridinium chloride against listeria monocytogenes and its influence on color and texture of cooked roast beef. | sliced (cut) and exterior (intact) surfaces of restructured cooked roast beef were inoculated with listeria monocytogenes, treated with cetylpyridinium chloride (cpc; immersion in 500 ml of 1% solution for 1 min), individually vacuum packaged, and stored for 42 days at 0 or 4 degrees c. noninoculated samples were similarly treated, packaged, and stored to determine effects on quality (color and firmness) and on naturally occurring bacterial populations, including aerobic plate counts and lactic ... | 2005 | 16300072 |
| listeria monocytogenes survival in refrigerator dill pickles. | listeria monocytogenes can survive and grow in refrigerated foods with ph values of approximately 4.0 to 5.0 and salt concentrations of 3 to 4%. home-fermented refrigerator dill pickles fit this description. contamination of this product with l. monocytogenes could cause serious problems because these items are not heated prior to consumption. l. monocytogenes survival and growth patterns were investigated in refrigerator dill pickles at 1.3, 3.8, and 7.6% salt concentrations. pickling cucumbers ... | 2005 | 16300073 |
| consequences of the development of nisin-resistant listeria monocytogenes in fermented dairy products. | wild listeria isolates representing serovars found in artisanal cheeses commercialized in asturias (northern spain) were assessed for their susceptibility to several bacteriocins. pediocin pa-1 was the most active bacteriocin followed by enterocin as-48, nisin, and plantaricin c. however, some listeria monocytogenes and listeria innocua strains were already highly resistant to pa-1. among the wild l. monocytogenes populations, the frequency of development of nisin resistance ranged from 10(-6) u ... | 2005 | 16300077 |
| thermal tolerance of acid-adapted and unadapted salmonella, escherichia coli o157:h7, and listeria monocytogenes in cantaloupe juice and watermelon juice. | a study was performed to determine d values of acid-adapted and unadapted cells of salmonella, escherichia coli o157:h7, and listeria monocytogenes in cantaloupe juice and watermelon juice. | 2005 | 16305669 |
| [experimental study on impact of salmon milt dna on t-cellular immunity]. | the impact of a biologically active food supplement containing salmon milt dna on cellular immunity was experimentally studied. it was shown that the dna had a dose-dependent stimulating effect on lymphocyte transformation, stimulated the dth response and protected from experimental listeriosis infection. | 2005 | 16308934 |
| retinoic acid amplifies the host immune response to lps through increased t lymphocytes number and lps binding protein expression. | vitamin a deficiency is associated with increased susceptibility to infection but the effects of vitamin a supplementation on host response to pathogens are controversial. this study investigated the mechanisms by which all-trans retinoic acid (atra) modulates the host immune response in an experimental model of vitamin a supplementation before and after challenge with lps in rats. we show here that a supplementation with five daily injections of 10mg/kg atra increased the number of t lymphocyte ... | 2005 | 16309824 |
| detection of listeria monocytogenes and the toxin listeriolysin o in food. | listeria monocytogenes is an emerging bacterial foodborne pathogen responsible for listeriosis, an illness characterized by meningitis, encephalitis, and septicaemia. less commonly, infection can result in cutaneous lesions and flu-like symptoms. in pregnant women, the pathogen can cause bacteraemia, and stillbirth or premature birth of the fetus. the mortality rate for those contracting listeriosis is approximately 20%. currently, the united states has a zero tolerance policy regarding the pres ... | 2006 | 16310269 |
| establishment of an experimental system allowing immobilization of proteins on the surface of bacillus subtilis cells. | gram-positive bacteria code for one or more enzymes termed sortases which catalyze the covalent anchoring of substrate proteins on their cell wall. they recognize an amino acid sequence designated sorting motif, present close to the c-terminal end of the substrate proteins, cleave within this motif and catalyze anchoring of the polypeptide chain to the peptide crossbridge linking the peptidoglycan strands in a transpeptidation reaction. bacillus subtilis has been reported to code for two differe ... | 2006 | 16310271 |
| il-12-assisted immunization against listeria monocytogenes using replication-restricted vsv-based vectors. | co-administration of il-12 with vaccine immunogens has proven to be an effective strategy for eliciting potent th1-biased immunity. unfortunately, the use of il-12 as a vaccine component has been limited because it is unstable at ambient temperatures, expensive to produce, and toxic when administered at excessive dosages. using reverse genetics, we created a recombinant replication-restricted vesicular stomatitis virus that expresses large quantities of an il-12 fusion protein (vsvdeltag-il12f), ... | 2006 | 16310294 |
| evaluation of chromogenic media for the detection of listeria species in food. | the aim of this study was to evaluate the performance of chromogenic agars, agar listeria according to ottaviani and agosti (aloa) and rapid l. mono agar, compared with oxford agar for the enumeration and detection of listeria species in food. | 2005 | 16313406 |
| environmental factors influencing the relationship between optical density and cell count for listeria monocytogenes. | the effect of temperature (2-30 degrees c), ph (4.8-7.4) and water activity (0.946-0.995) on the relationship between optical density (od) at 600 nm and the plate count (cfu ml(-1)) was investigated for listeria monocytogenes. | 2005 | 16313423 |
| anti-bacterial activity of lactobacillus plantarum strain sk1 against listeria monocytogenes is due to lactic acid production. | the aim of this research was to investigate the potential of lactobacillus plantarum strain sk1 for use as a biological control agent against listeria monocytogenes and determine its mechanism of anti-listerial activity. | 2005 | 16313424 |
| membrane tumor necrosis factor confers partial protection to listeria infection. | tumor necrosis factor (tnf) plays a critical role in the host response to the intracellular pathogen listeria monocytogenes (lm). tnf exists in soluble and membrane-bound forms and exhibits both unique and overlapping activities. we examined the role of membrane tnf in the absence of secreted tnf for host resistance in knockin mice in which the endogenous tnf was replaced by a regulated, noncleavable allele (mem-tnf). macrophages expressing mem-tnf produced nitric oxide and displayed normal bact ... | 2005 | 16314479 |
| identification of unusual bacterial glycosylation by tandem mass spectrometry analyses of intact proteins. | the characterization of protein glycosylation can be a complex and time-consuming procedure, especially for prokaryote o-linked glycoproteins, which often comprise unusual oligosaccharide structures with no known glycosylation motif. in this report, we describe a "top-down" approach that provides information on the extent of glycosylation, the molecular masses, and the structure of oligosaccharide residues on bacterial flagella, important structural proteins involved in the motility of pathogeni ... | 2005 | 16316188 |
| monitoring changes in nisin susceptibility of listeria monocytogenes scott a as an indicator of growth phase using facs. | listeria monocytogenes has previously been shown to adapt to a wide variety of environmental niches, principally those associated with low ph, and this compromises its control in food environments. an understanding of the mechanism(s) by which l. monocytogenes survives unfavourable environmental conditions will aid in developing new food processing methods to control the organism in foodstuffs. the present study aimed to gain a further understanding of the physiological basis for the differentia ... | 2006 | 16316702 |
| the activity of pathway-selective estrogen receptor ligands in experimental septic shock. | estrogen receptors (er) are widely expressed in multiple genital and nongenital tissues. upon engagement of these receptors, multiple genes are affected in target tissues via estrogen response elements. nonsteroidal pathway-selective er ligands have recently been identified that inhibit nf-kappab transcriptional activity and are devoid of conventional estrogenic activities on genital tissues. these pathway-selective ligands are potent anti-inflammatory agents in vivo and may prove to be of thera ... | 2005 | 16317384 |
| [misleading presentation of rhombencephalitis due to listeria monocytogenes]. | rhombencephalitis due to listeria monocytogenes may present in a variety of forms. | 2005 | 16317592 |
| nationwide outbreak of listeriosis due to contaminated meat. | we used molecular subtyping to investigate an outbreak of listeriosis involving residents of 24 us states. we defined a case as infection with listeria monocytogenes serotype 4b yielding one of several closely related patterns when subtyped by pulsed-field gel electrophoresis. patients infected with strains yielding different patterns were used as controls. a total of 108 cases were identified with 14 associated deaths and four miscarriages or stillbirths. a case-control study implicated meat fr ... | 2006 | 16318652 |
| gastrointestinal infections in immunocompromised hosts. | gastrointestinal infections in the immunocompromised host continue to have significant morbidity and mortality throughout the world. they all have similar exposures to viruses, bacteria and parasites and respond to these infections in a similar way. this review will summarize the latest reports on the epidemiology, diagnosis and treatment of known and emerging infections over the last 12 months. | 2006 | 16319672 |
| simultaneous deficiency of both mura and p60 proteins generates a rough phenotype in listeria monocytogenes. | we examined eight spontaneously occurring rough mutants of listeria monocytogenes for their ability to express two previously reported autolysins, p60 and mura. all mutants lack mura expression and show strongly reduced levels of extracellular p60. one rough strain harbors a variant of the p60 protein with a partially truncated catalytic domain. in seven cases there were shifts in the localization of p60 to the membrane fraction. mutations within the seca2 gene, encoding an auxiliary protein sec ... | 2005 | 16321943 |
| immunosuppression related to collagen-vascular disease or its treatment. | collagen-vascular diseases are associated with immune dysregulation and inflammation, leading to tissue destruction or compromise. immunosuppression is more commonly associated with the drugs used to treat these disorders than with the diseases themselves. the newest agents being used to treat collagen-vascular diseases are the tumor necrosis factor (tnf)-alpha inhibitors. u.s. food and drug administration-approved tnf-alpha inhibitors have differing effects on the immune system, reflecting thei ... | 2005 | 16322600 |
| multistate outbreak of listeriosis linked to turkey deli meat and subsequent changes in us regulatory policy. | listeriosis, a life-threatening foodborne illness caused by listeria monocytogenes, affects approximately 2500 americans annually. between july and october 2002, an uncommon strain of l. monocytogenes caused an outbreak of listeriosis in 9 states. | 2006 | 16323088 |
| sources of interferon-gamma (ifn-gamma) in early immune response to listeria monocytogenes. | early, innate production of interferon-gamma (ifn-gamma) is a critical step in immunological defense against certain pathogens such as intracellular bacteria (e.g. listeria monocytogenes), viruses and fungi. while activated t cells and activated natural killer (nk) cells were initially thought to be the only relevant source of ifn-gamma, macrophages (mphi) and dendritic cells can also be stimulated to produce ifn-gamma in vitro under certain conditions. however, a convincing analysis at single c ... | 2005 | 16323704 |
| behaviour of the pathogen surrogates listeria innocua and clostridium sporogenes during production of parsley in fields fertilized with contaminated amendments. | the survival and transfer of listeria innocua and clostridium sporogenes, used as surrogates of the food borne pathogens listeria monocytogenes and clostridium botulinum, were quantitatively assessed under field conditions. in the soil, spores of c. sporogenes declined by less than 0.7 log cycles within 16 months and were detected on parsley leaves throughout the experiment. in contrast, l. innocua in the soil declined by 7 log cycles in 90 days and was detected on leaves in low numbers (>0.04 m ... | 2005 | 16332327 |
| effect of immersion solutions containing enterocin as-48 on listeria monocytogenes in vegetable foods. | the effect of immersion solutions containing enterocin as-48 alone or in combination with chemical preservatives on survival and proliferation of listeria monocytogenes cect 4032 inoculated on fresh alfalfa sprouts, soybean sprouts, and green asparagus was tested. immersion treatments (5 min at room temperature) with as-48 solutions (25 microg/ml) reduced listeria counts of artificially contaminated alfalfa and soybean sprouts by approximately 2.0 to 2.4 log cfu/g compared to a control immersion ... | 2005 | 16332751 |
| triphenylmethane reductase from citrobacter sp. strain kctc 18061p: purification, characterization, gene cloning, and overexpression of a functional protein in escherichia coli. | we purified to homogeneity an enzyme from citrobacter sp. strain kctc 18061p capable of decolorizing triphenylmethane dyes. the native form of the enzyme was identified as a homodimer with a subunit molecular mass of about 31 kda. it catalyzes the nadh-dependent reduction of triphenylmethane dyes, with remarkable substrate specificity related to dye structure. maximal enzyme activity occurred at ph 9.0 and 60 degrees c. the enzymatic reaction product of the triphenylmethane dye crystal violet wa ... | 2005 | 16332773 |
| exogenous isoleucine and fatty acid shortening ensure the high content of anteiso-c15:0 fatty acid required for low-temperature growth of listeria monocytogenes. | previous studies have demonstrated that the branched-chain fatty acid anteiso-c15:0 plays a critical role in the growth of listeria monocytogenes at low temperatures by ensuring sufficient membrane fluidity. studies utilizing a chemically defined minimal medium revealed that the anteiso fatty acid precursor isoleucine largely determined the fatty acid profile and fatty acid response of the organism to lowered growth temperature. when isoleucine was sufficient, the fatty acid profile was very uni ... | 2005 | 16332779 |
| relatedness of listeria monocytogenes isolates recovered from selected ready-to-eat foods and listeriosis patients in the united states. | pulsed-field gel electrophoresis and serotyping were performed for 544 isolates of listeria monocytogenes, including 502 isolates recovered from contaminated samples from 31,705 retail ready-to-eat (rte) food products and 42 isolates recovered from human cases of listeriosis. the isolates were from maryland (294 isolates) and california (250 isolates) and were collected in 2000 and 2001. the isolates were placed into 16 asci pulsogroups (level of relatedness within each group, > or =66%), 139 as ... | 2005 | 16332793 |
| listeria monocytogenes perr mutants display a small-colony phenotype, increased sensitivity to hydrogen peroxide, and significantly reduced murine virulence. | deletion of perr in listeria monocytogenes results in a small-colony phenotype (deltaperrsm) that is slow growing and exhibits increased sensitivity to h2o2. at a relatively high frequency, large-colony variants (deltaperrlg) arise, which are more resistant to h2o2 than the wild-type and ultimately dominate the culture. transcriptional analysis revealed that the kat gene (catalase) is up-regulated in both types of mutants and that the highest level is apparent in deltaperrsm mutants, demonstrati ... | 2005 | 16332818 |
| contingency locus in ctsr of listeria monocytogenes scott a: a strategy for occurrence of abundant piezotolerant isolates within clonal populations. | in a recent study we demonstrated that a high-hydrostatic-pressure-tolerant isolate of listeria monocytogenes lacks a codon in the class 3 heat shock regulator gene ctsr. this mutation in the region that encodes four consecutive glycines was directly responsible for the observed piezotolerance, increased stress resistance, and reduced virulence. the aim of the present study was to determine whether mutations in ctsr are frequently associated with piezotolerance in l. monocytogenes. wild-type cul ... | 2005 | 16332826 |
| select listeria monocytogenes subtypes commonly found in foods carry distinct nonsense mutations in inla, leading to expression of truncated and secreted internalin a, and are associated with a reduced invasion phenotype for human intestinal epithelial cells. | the surface protein internalin a (inla) contributes to the invasion of human intestinal epithelial cells by listeria monocytogenes. screening of l. monocytogenes strains isolated from human clinical cases (n=46), foods (n=118), and healthy animals (n=58) in the united states revealed mutations in inla leading to premature stop codons (pmscs) in l. monocytogenes ribotypes dup-1052a and dup-16635a (pmsc mutation type 1), dup-1025a and dup-1031a (pmsc mutation type 2), and dup-1046b and dup-1062a ( ... | 2005 | 16332872 |
| a novel real-time pcr for listeria monocytogenes that monitors analytical performance via an internal amplification control. | we describe a novel quantitative real-time (q)-pcr assay for listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (iac). the iac is a chimeric double-stranded dna containing a fragment of the rapeseed bnaccg8 gene flanked by the hly-specific target sequences. this iac is detected using a second taqman probe labeled with a different fluorophore, enabling the simultaneous monitoring of the hly and iac signals. the hly-iac assay had ... | 2005 | 16332910 |
| [evaluation of the antimicrobial action of honey against staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, salmonella enteritidis, listeria monocytogenes and aspergillus niger. evaluation of its microbiological charge]. | the evaluation of the microbiological charge present in costa rican samples as the evaluation of its antimicrobial activity over different microorganisms, including those associated to wound infections, will allow to emit criteria referred to its use in therapeutic treatments, specially as alternative therapy for cases involving antibiotic resistant bacteria. the microbiological charge of 25 honey samples, acquired in costa rican markets was evaluated through several indicators including total p ... | 2005 | 16335227 |
| characterization of the bifunctional gamma-glutamate-cysteine ligase/glutathione synthetase (gshf) of pasteurella multocida. | glutamate-cysteine ligase (gamma-ecl) and glutathione synthetase (gs) are the two unrelated ligases that constitute the glutathione biosynthesis pathway in most eukaryotes, purple bacteria, and cyanobacteria. gamma-ecl is a member of the glutamine synthetase family, whereas gs enzymes group together with highly diverse carboxyl-to-amine/thiol ligases, all characterized by the so-called two-domain atp-grasp fold. this generalized scheme toward the formation of glutathione, however, is incomplete, ... | 2006 | 16339152 |
| selective plating medium for quantitative recovery of food-borne listeria monocytogenes. | [this corrects the article on p. 167 in vol. 56.]. | 1990 | 16348168 |
| enhanced control of listeria monocytogenes by in situ-produced pediocin during dry fermented sausage production. | [this corrects the article on p. 887 in vol. 58.]. | 1992 | 16348726 |
| common mechanistic action of bacteriocins from lactic acid bacteria. | the influence of four bacteriocins from lactic acid bacteria on the proton motive force (pmf) of sensitive organisms was investigated. pediocin pa-1 (20 mug/ml) and leuconocin s (48.5 mug/ml) mediated total or major pmf dissipation of energized listeria monocytogenes scott a cells in a concentration-dependent manner, as has been shown for nisin. lactacin f (13.5 mug/ml) caused 87% pmf depletion of energized lactobacillus delbrueckii atcc 4797 cells, also in a concentration-dependent fashion. the ... | 1993 | 16349044 |
| characterization by multilocus enzyme electrophoresis of listeria monocytogenes isolates involved in ovine listeriosis outbreaks in scotland from 1989 to 1991. | initial results from a study of five small ovine listeriosis outbreaks in scotland in 1989 to 1991 are presented. forty-eight isolates including three from silage were typed at 10 polymorphic enzyme loci by using multilocus enzyme electrophoresis resulting in the identification of 12 electrophoretic types. phylogenetic analysis partitioned the 12 electrophoretic types into two statistically distinct divisions distinguishing 1/2a serotypes from non-1/2a serotypes. | 1993 | 16349052 |
| a simple rna probe system for analysis of listeria monocytogenes polymerase chain reaction products. | [this corrects the article on p. 2796 in vol. 59.]. | 1994 | 16349203 |
| mode of action of nisin z against listeria monocytogenes scott a grown at high and low temperatures. | nisin z, a natural nisin variant, was recently isolated from lactococcus lactis subspecies lactis nizo 22186. the gene for this lantibiotic, designated nisz, has been cloned, and its nucleotide sequence was found to be identical to that of the precursor nisin gene with the exception of a single mutation resulting in the substitution of asn-27 for his-27 in the mature polypeptide (j. w. m. mulders, i. j. boerrigter, h. s. rollema, r. j. siezen, and w. m. de vos, eur. j. biochem. 201:581-584, 1991 ... | 1994 | 16349286 |
| inducing l forms in listeria monocytogenes types 1 through 7. | 1967 | 16349782 | |
| listeria monocytogenes, a food-borne pathogen. | [this corrects the article on p. 485 in vol. 55.]. | 1991 | 16350251 |
| evaluation of farm management practices as risk factors for clinical listeriosis and fecal shedding of listeria monocytogenes in ruminants. | to assess seasonal variation in prevalence of listeria monocytogenes on ruminant farms and identify management practices associated with ruminant listeriosis and fecal shedding of l. monocytogenes. | 2005 | 16350271 |
| [meningoencephalitis by listeria in the lupus disease]. | we present a patient with lupus nephropathy of 20 years of evolution in treatment with oral steroids who developed a meningoencephalitis associated to bacteraemia by listeria monocytogenes. the patient was treated successfully with gentamicin and ampicillin for 6 weeks. infection by listeria monocytogenes occurs more frequently in individuals with some form of immunodeficiency like lupus disease, with a mortality around 30%. | 2005 | 16351491 |
| listeria monocytogenes as the possible cause of the spontanous abortion in female of the fertile age. | listeria monocytogenes is a gram-positive, weakly pathogenic bacterium able to grow also at the temperature of 40 degrees c. a man most often gets affected by consuming contaminated food and water. animals can carry bacteria although they have not to appear ill, then their meat and milk products are the source of infection of human being. the disease most often attacks with the weak immune system, newborns and pregnant women. that what is significant, listeria penetrates through the placenta and ... | 2005 | 16351605 |
| ccpc-dependent regulation of citb and lmo0847 in listeria monocytogenes. | in bacillus subtilis, the catabolite control protein c (ccpc) plays a critical role in regulating the genes encoding the enzymes of the tricarboxylic acid branch of the krebs citric acid cycle. a gene encoding a potential ccpc homolog and two potential target genes were identified in the listeria monocytogenes genome. in vitro gel mobility shift assays and dnase i footprinting experiments showed that l. monocytogenes ccpc (ccpc(lm)) interacts with the promoter regions of citb(lm) (the gene that ... | 2006 | 16352834 |