Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| transcriptome profiling of huanglongbing (hlb) tolerant and susceptible citrus plants reveals the role of basal resistance in hlb tolerance. | huanglongbing (hlb) is currently the most destructive disease of citrus worldwide. although there is no immune cultivar, field tolerance to hlb within citrus and citrus relatives has been observed at the usda picos farm at ft. pierce, florida, where plants have been exposed to a very high level of hlb pressure since 2006. in this study, we used rna-seq to evaluate expression differences between two closely related cultivars after hlb infection: hlb-tolerant "jackson" grapefruit-like-hybrid trees ... | 2016 | 27446161 |
| a genome-wide analysis reveals stress and hormone responsive patterns of tify family genes in brassica rapa. | the tify family is a plant-specific group of proteins with a diversity of functions and includes four subfamilies, viz. zml, tify, ppd, and jasmonate zim-domain (jaz) proteins. tify family members, particularly jaz subfamily proteins, play roles in biological processes such as development and stress and hormone responses in arabidopsis, rice, chickpea, and grape. however, there is no information about this family in any brassica crop. this study identifies 36 tify genes in brassica rapa, an econ ... | 2016 | 27446164 |
| visualizing the phage t4 activated transcription complex of dna and e. coli rna polymerase. | the ability of rna polymerase (rnap) to select the right promoter sequence at the right time is fundamental to the control of gene expression in all organisms. however, there is only one crystallized structure of a complete activator/rnap/dna complex. in a process called σ appropriation, bacteriophage t4 activates a class of phage promoters using an activator (mota) and a co-activator (asia), which function through interactions with the σ(70) subunit of rnap. we have developed a holistic, struct ... | 2016 | 27458207 |
| a de novo-assembly based data analysis pipeline for plant obligate parasite metatranscriptomic studies. | current and emerging plant diseases caused by obligate parasitic microbes such as rusts, downy mildews, and powdery mildews threaten worldwide crop production and food safety. these obligate parasites are typically unculturable in the laboratory, posing technical challenges to characterize them at the genetic and genomic level. here we have developed a data analysis pipeline integrating several bioinformatic software programs. this pipeline facilitates rapid gene discovery and expression analysi ... | 2016 | 27462318 |
| a clade-specific arabidopsis gene connects primary metabolism and senescence. | nearly immobile, plants have evolved new components to be able to respond to changing environments. one example is qua quine starch (qqs, at3g30720), an arabidopsis thaliana-specific orphan gene that integrates primary metabolism with adaptation to environment changes. saqr (senescence-associated and qqs-related, at1g64360), is unique to a clade within the family brassicaceae; as such, the gene may have arisen about 20 million years ago. saqr is up-regulated in qqs rnai mutant and in the apx1 mu ... | 2016 | 27462324 |
| xanthomonas whole genome sequencing: phylogenetics, host specificity and beyond. | 2016 | 27470197 | |
| surface immobilization of human arginase-1 with an engineered ice nucleation protein display system in e. coli. | ice nucleation protein (inp) is frequently used as a surface anchor for protein display in gram-negative bacteria. here, male and tora signal peptides, and three charged polypeptides, 6×lys, 6×glu and 6×asp, were anchored to the n-terminus of truncated inp (inak-n) to improve its surface display efficiency for human arginase1 (arg1). our results indicated that the tora signal peptide increased the surface translocation of non-protein fused inak-n and human arg1 fused inak-n (inak-n/arg1) by 80.7 ... | 2016 | 27479442 |
| pathogen infection and morc proteins affect chromatin accessibility of transposable elements and expression of their proximal genes in arabidopsis. | to assess the role of morc1 in epigenetics in relation to plant immunity, genome-wide chromatin accessibility was compared between mock- or pseudomonas syringae pv. tomato-inoculated wild type (wt) arabidopsis, the morc1/2 double mutant, or both. most changes in chromatin accessibility, scored by dnase i hypersensitive sites (dhss), were located in the promoters of genes and transposable elements (tes). comparisons between morc1/2 and wt receiving the same treatment revealed differential dhss (d ... | 2016 | 27482822 |
| cold adaptation regulated by cryptic prophage excision in shewanella oneidensis. | among the environmental stresses experienced by bacteria, temperature shifts are one of the most important. in this study, we discovered a novel cold adaptation mechanism in shewanella oneidensis that occurs at the dna level and is regulated by cryptic prophage excision. previous studies on bacterial cold tolerance mainly focus on the structural change of cell membrane and changes at the rna and protein levels. whether or not genomic change can also contribute to this process has not been explor ... | 2016 | 27482926 |
| identification of differentially-expressed genes in response to mycosphaerella fijiensis in the resistant musa accession 'calcutta-4' using suppression subtractive hybridization. | bananas and plantains are considered an important crop around the world. banana production is affected by several constraints, of which black sigatoka disease, caused by the fungus mycosphaerella fijiensis, is considered one of the most important diseases in banana plantations. the banana accession 'calcutta-4' has a natural resistance to black sigatoka; however, the fruit is not valuable for commercialization. gene identification and expression studies in 'calcutta-4' might reveal possible gene ... | 2016 | 27487237 |
| expression of a grapevine nac transcription factor gene is induced in response to powdery mildew colonization in salicylic acid-independent manner. | tissue colonization by grape powdery mildew (pm) pathogen erysiphe necator (schw.) burr triggers a major remodeling of the transcriptome in the susceptible grapevine vitis vinifera l. while changes in the expression of many genes bear the signature of salicylic acid (sa) mediated regulation, the breadth of pm-induced changes suggests the involvement of additional regulatory networks. to explore pm-associated gene regulation mediated by other sa-independent systems, we designed a microarray exper ... | 2016 | 27488171 |
| the protein elicitor pevd1 enhances resistance to pathogens and promotes growth in arabidopsis. | the protein elicitor pevd1, isolated from verticillium dahlia, could enhance resistance to tmv in tobacco and verticillium wilt in cotton. here, the pevd1 gene was over-expressed in wild type (wt) arabidopsis, and its biological functions were investigated. our results showed that the transgenic lines were more resistant to botrytis cinerea and pseudomonas syringae pv. tomato dc3000 than the wt line was. in transgenic plants, both the germination time and bolting time required were significantly ... | 2016 | 27489497 |
| modification of bacterial effector proteins inside eukaryotic host cells. | pathogenic bacteria manipulate their hosts by delivering a number of virulence proteins -called effectors- directly into the plant or animal cells. recent findings have shown that such effectors can suffer covalent modifications inside the eukaryotic cells. here, we summarize the recent reports where effector modifications by the eukaryotic machinery have been described. we restrict our focus on proteins secreted by the type iii or type iv systems, excluding other bacterial toxins. we describe t ... | 2016 | 27489796 |
| a low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host. | the co-evolution of bacterial plant pathogens and their hosts is a complex and dynamic process. host resistance imposes stress on invading pathogens that can lead to changes in the bacterial genome enabling the pathogen to escape host resistance. we have observed this phenomenon with the plant pathogen pseudomonas syringae pv. phaseolicola where isolates that have lost the genomic island pphgi-1 carrying the effector gene avrpphb from its chromosome are infective against previously resistant pla ... | 2016 | 27491006 |
| visual analysis for detection and quantification of pseudomonas cichorii disease severity in tomato plants. | pathogen infection in plants induces complex responses ranging from gene expression to metabolic processes in infected plants. in spite of many studies on biotic stress-related changes in host plants, little is known about the metabolic and phenotypic responses of the host plants to pseudomonas cichorii infection based on image-based analysis. to investigate alterations in tomato plants according to disease severity, we inoculated plants with different cell densities of p. cichorii using dipping ... | 2016 | 27493605 |
| identification and expression profiles of six transcripts encoding carboxylesterase protein in vitis flexuosa infected with pathogens. | plants protect themselves from pathogen attacks via several mechanisms, including hypersensitive cell death. recognition of pathogen attack by the plant resistance gene triggers expression of carboxylesterase genes associated with hypersensitive response. we identified six transcripts of carboxylesterase genes, vitis flexuosa carboxylesterase 5585 (vfcxe5585), vfcxe12827, vfcxe13132, vfcxe17159, vfcxe18231, and vfcxe47674, which showed different expression patterns upon transcriptome analysis of ... | 2016 | 27493610 |
| a rice gene homologous to arabidopsis agd2-like defense1 participates in disease resistance response against infection with magnaporthe oryzae. | ald1 (aberrant growth and death2 [agd2]-like defense1) is one of the key defense regulators in arabidopsis thaliana and nicotiana benthamiana. in these model plants, ald1 is responsible for triggering basal defense response and systemic resistance against bacterial infection. as well ald1 is involved in the production of pipecolic acid and an unidentified compound(s) for systemic resistance and priming syndrome, respectively. these previous studies proposed that ald1 is a potential candidate for ... | 2016 | 27493611 |
| antibacterial activity of cinnamaldehyde and estragole extracted from plant essential oils against pseudomonas syringae pv. actinidiae causing bacterial canker disease in kiwifruit. | pseudomonas syringae pv. actinidiae (psa) causes bacterial canker disease in kiwifruit. antibacterial activity of plant essential oils (peos) originating from 49 plant species were tested against psa by a vapor diffusion and a liquid culture assays. the five peos from pimenta racemosa, p. dioica, melaleuca linariifolia, m. cajuputii, and cinnamomum cassia efficiently inhibited psa growth by either assays. among their major components, estragole, eugenol, and methyl eugenol showed significant ant ... | 2016 | 27493612 |
| multiple evolutionary events involved in maintaining homologs of resistance to powdery mildew 8 in brassica napus. | the resistance to powdery mildew 8 (rpw8) locus confers broad-spectrum resistance to powdery mildew in arabidopsis thaliana. there are four homologous to rpw8s (brhrs) in brassica rapa and three in brassica oleracea (bohrs). brassica napus (bn) is derived from diploidization of a hybrid between b. rapa and b. oleracea, thus should have seven homologs of rpw8 (bnhrs). it is unclear whether these genes are still maintained or lost in b. napus after diploidization and how they might have been evolv ... | 2016 | 27493652 |
| detecting n-myristoylation and s-acylation of host and pathogen proteins in plants using click chemistry. | the plant plasma membrane is a key battleground in the war between plants and their pathogens. plants detect the presence of pathogens at the plasma membrane using sensor proteins, many of which are targeted to this lipophilic locale by way of fatty acid modifications. pathogens secrete effector proteins into the plant cell to suppress the plant's defense mechanisms. these effectors are able to access and interfere with the surveillance machinery at the plant plasma membrane by hijacking the hos ... | 2016 | 27493678 |
| the arabidopsis protein phosphatase pp2c38 negatively regulates the central immune kinase bik1. | plants recognize pathogen-associated molecular patterns (pamps) via cell surface-localized pattern recognition receptors (prrs), leading to prr-triggered immunity (pti). the arabidopsis cytoplasmic kinase bik1 is a downstream substrate of several prr complexes. how plant pti is negatively regulated is not fully understood. here, we identify the protein phosphatase pp2c38 as a negative regulator of bik1 activity and bik1-mediated immunity. pp2c38 dynamically associates with bik1, as well as with ... | 2016 | 27494702 |
| bacterial ice nucleation in monodisperse d2o and h2o-in-oil emulsions. | ice nucleation is of fundamental significance in many areas, including atmospheric science, food technology, and cryobiology. in this study, we investigated the ice-nucleation characteristics of picoliter-sized drops consisting of different d2o and h2o mixtures with and without the ice-nucleating bacteria pseudomonas syringae. we also studied the effects of commonly used cryoprotectants such as ethylene glycol, propylene glycol, and trehalose on the nucleation characteristics of d2o and h2o mixt ... | 2016 | 27495973 |
| effectors from wheat rust fungi suppress multiple plant defense responses. | fungi that cause cereal rust diseases (genus puccinia) are important pathogens of wheat globally. upon infection, the fungus secretes a number of effector proteins. although a large repository of putative effectors has been predicted using bioinformatic pipelines, the lack of available high-throughput effector screening systems has limited functional studies on these proteins. in this study, we mined the available transcriptomes of puccinia graminis and p. striiformis to look for potential effec ... | 2017 | 27503371 |
| fine mapping and identification of candidate genes for a qtl affecting meloidogyne incognita reproduction in upland cotton. | the southern root-knot nematode (meloidogyne incognita; rkn) is one of the most important economic pests of upland cotton (gossypium hirsutum l.). host plant resistance, the ability of a plant to suppress nematode reproduction, is the most economical, practical, and environmentally sound method to provide protection against this subterranean pest. the resistant line auburn 623rnr and a number of elite breeding lines derived from it remain the most important source of root-knot nematode (rkn) res ... | 2016 | 27503539 |
| proteometabolomic study of compatible interaction in tomato fruit challenged with sclerotinia rolfsii illustrates novel protein network during disease progression. | fruit is an assimilator of metabolites, nutrients, and signaling molecules, thus considered as potential target for pathogen attack. in response to patho-stress, such as fungal invasion, plants reorganize their proteome, and reconfigure their physiology in the infected organ. this remodeling is coordinated by a poorly understood signal transduction network, hormonal cascades, and metabolite reallocation. the aim of the study was to explore organ-based proteomic alterations in the susceptibility ... | 2016 | 27507973 |
| the root hair specific syp123 regulates the localization of cell wall components and contributes to rizhobacterial priming of induced systemic resistance. | root hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. root hairs are tubular-shaped outgrowths that emerge from trichoblasts. this polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. members of the syntaxin family of snares (soluble n-ethylmaleimide-sensitive factor attachment protein receptor) in plants (syp), have been implicated in re ... | 2016 | 27507978 |
| pseudomonas lini strain zbg1 revealed carboxylic acid utilization and copper resistance features required for adaptation to vineyard soil environment: a draft genome analysis. | pseudomonas lini strain zbg1 was isolated from the soil of vineyard in zellenberg, france and the draft genome was reported in this study. bioinformatics analyses of the genome revealed presence of genes encoding tartaric and malic acid utilization as well as copper resistance that correspond to the adaptation this strain in vineyard soil environment. | 2016 | 27512520 |
| nudix effectors: a common weapon in the arsenal of plant pathogens. | 2016 | 27513453 | |
| posttranslational modifications of npr1: a single protein playing multiple roles in plant immunity and physiology. | 2016 | 27513560 | |
| pseudomonas syringae differentiates into phenotypically distinct subpopulations during colonization of a plant host. | bacterial microcolonies with heterogeneous sizes are formed during colonization of phaseolus vulgaris by pseudomonas syringae. heterogeneous expression of structural and regulatory components of the p. syringae type iii secretion system (t3ss), essential for colonization of the host apoplast and disease development, is likewise detected within the plant apoplast. t3ss expression is bistable in the homogeneous environment of nutrient-limited t3ss-inducing medium, suggesting that subpopulation for ... | 2016 | 27516206 |
| structure of a pathogen effector reveals the enzymatic mechanism of a novel acetyltransferase family. | effectors secreted by the type iii secretion system are essential for bacterial pathogenesis. members of the yersinia outer-protein j (yopj) family of effectors found in diverse plant and animal pathogens depend on a protease-like catalytic triad to acetylate host proteins and produce virulence. however, the structural basis for this noncanonical acetyltransferase activity remains unknown. here, we report the crystal structures of the yopj effector hopz1a, produced by the phytopathogen pseudomon ... | 2016 | 27525589 |
| differential control and function of arabidopsis prodh1 and prodh2 genes on infection with biotrophic and necrotrophic pathogens. | arabidopsis contains two proline dehydrogenase (prodh) genes, prodh1 and prodh2, encoding for homologous and functional isoenzymes. although prodh1 has been studied extensively, especially under abiotic stress, prodh2 has only started to be analysed in recent years. these genes display distinctive expression patterns and show weak transcriptional co-regulation, but are both activated in pathogen-infected tissues. we have demonstrated previously that arabidopsis plants with silenced prodh1/2 expr ... | 2016 | 27526663 |
| behind the lines-actions of bacterial type iii effector proteins in plant cells. | pathogenicity of most gram-negative plant-pathogenic bacteria depends on the type iii secretion (t3s) system, which translocates bacterial effector proteins into plant cells. type iii effectors modulate plant cellular pathways to the benefit of the pathogen and promote bacterial multiplication. one major virulence function of type iii effectors is the suppression of plant innate immunity, which is triggered upon recognition of pathogen-derived molecular patterns by plant receptor proteins. type ... | 2016 | 27526699 |
| a set of pcrs for rapid identification and characterization of pseudomonas syringae phylogroups. | 2016 | 27528015 | |
| overexpression of the eggplant (solanum melongena) nac family transcription factor smnac suppresses resistance to bacterial wilt. | bacterial wilt (bw) is a serious disease that affects eggplant (solanum melongena) production. although resistance to this disease has been reported, the underlying mechanism is unknown. in this study, we identified a nac family transcription factor (smnac) from eggplant and characterized its expression, its localization at the tissue and subcellular levels, and its role in bw resistance. to this end, transgenic eggplant lines were generated in which the expression of smnac was constitutively up ... | 2016 | 27528282 |
| multiple xanthomonas euvesicatoria type iii effectors inhibit flg22-triggered immunity. | xanthomonas euvesicatoria is the causal agent of bacterial spot disease in pepper and tomato. x. euvesicatoria bacteria interfere with plant cellular processes by injecting effector proteins into host cells through the type iii secretion (t3s) system. about 35 t3s effectors have been identified in x. euvesicatoria 85-10, and a few of them were implicated in suppression of pattern-triggered immunity (pti). we used an arabidopsis thaliana pathogen-free protoplast-based assay to identify x. euvesic ... | 2016 | 27529660 |
| comprehensive analysis of the membrane phosphoproteome regulated by oligogalacturonides in arabidopsis thaliana. | early changes in the arabidopsis thaliana membrane phosphoproteome in response to oligogalacturonides (ogs), a class of plant damage-associated molecular patterns (damps), were analyzed by two complementary proteomic approaches. differentially phosphorylated sites were determined through phosphopeptide enrichment followed by lc-ms/ms using label-free quantification; differentially phosphorylated proteins were identified by 2d-dige combined with phospho-specific fluorescent staining (phospho-dige ... | 2016 | 27532006 |
| the potato aphid salivary effector me47 is a glutathione-s-transferase involved in modifying plant responses to aphid infestation. | polyphagous aphid pests cause considerable economic damage to crop plants, primarily through the depletion of photoassimilates and transfer of viruses. the potato aphid (macrosiphum euphorbiae) is a notable pest of solanaceous crops, however, the molecular mechanisms that underpin the ability to colonize these hosts are unknown. it has recently been demonstrated that like other aphid species, m. euphorbiae injects a battery of salivary proteins into host plants during feeding. it is speculated t ... | 2016 | 27536306 |
| modulation of host mirnas by intracellular bacterial pathogens. | micrornas (mirnas) are short non-coding rnas that regulate the expression of protein coding genes of viruses and eukaryotes at the post-transcriptional level. the eukaryotic genes regulated by mirnas include those whose products are critical for biological processes such as cell proliferation, metabolic pathways, immune response, and development. it is now increasingly recognized that modulation of mirnas associated with biological processes is one of the strategies adopted by bacterial pathogen ... | 2016 | 27536558 |
| genome-wide analysis and evolution of the pto-like protein kinase (plpk) gene family in pepper. | the tomato pto gene, which encodes a serine/threonine kinase (stk) domain-containing protein, confers resistance to bacterial speck disease caused by pseudomonas syringae pv. tomato (pst). in this study, in vivo recognition assays using pvx constructs showed that avrpto was specifically recognized in the pepper genotypes. this avrpto recognition caused a nonhost hypersensitive response (hr) and localization of the pvx::avrpto fusion protein to inoculated pepper leaf tissues, which indicates the ... | 2016 | 27536870 |
| virus-induced gene silencing-based functional analyses revealed the involvement of several putative trehalose-6-phosphate synthase/phosphatase genes in disease resistance against botrytis cinerea and pseudomonas syringae pv. tomato dc3000 in tomato. | trehalose and its metabolism have been demonstrated to play important roles in control of plant growth, development, and stress responses. however, direct genetic evidence supporting the functions of trehalose and its metabolism in defense response against pathogens is lacking. in the present study, genome-wide characterization of putative trehalose-related genes identified 11 sltpss for trehalose-6-phosphate synthase, 8 sltpps for trehalose-6-phosphate phosphatase and one sltre1 for trehalase i ... | 2016 | 27540389 |
| gall-id: tools for genotyping gall-causing phytopathogenic bacteria. | understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of dna sequences can be less straightforward to use. to address this, we developed gall-id, a web-based platform that uses dna sequence information from 16s rdna, mult ... | 2016 | 27547538 |
| transcriptome analysis of pseudomonas aeruginosa pao1 grown at both body and elevated temperatures. | functional genomics research can give us valuable insights into bacterial gene function. rna sequencing (rna-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. in this study, we used the rna-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen pseudomonas aeruginosa pao1 following heat shock. samples were grown at both the human body temperature (37 °c) and an arbitrarily-selected temperature of 46 °c. in this work ... | 2016 | 27547539 |
| a proposal for a genome similarity-based taxonomy for plant-pathogenic bacteria that is sufficiently precise to reflect phylogeny, host range, and outbreak affiliation applied to pseudomonas syringae sensu lato as a proof of concept. | taxonomy of plant pathogenic bacteria is challenging because pathogens of different crops often belong to the same named species but current taxonomy does not provide names for bacteria below the subspecies level. the introduction of the host range-based pathovar system in the 1980s provided a temporary solution to this problem but has many limitations. the affordability of genome sequencing now provides the opportunity for developing a new genome-based taxonomic framework. we already proposed t ... | 2017 | 27552324 |
| multi-environment qtl studies suggest a role for cysteine-rich protein kinase genes in quantitative resistance to blackleg disease in brassica napus. | resistance to the blackleg disease of brassica napus (canola/oilseed rape), caused by the hemibiotrophic fungal pathogen leptosphaeria maculans, is determined by both race-specific resistance (r) genes and quantitative resistance loci (qtl), or adult-plant resistance (apr). while the introgression of r genes into breeding material is relatively simple, qtl are often detected sporadically, making them harder to capture in breeding programs. for the effective deployment of apr in crop varieties, r ... | 2016 | 27553246 |
| root-mediated signal transmission of systemic acquired resistance against above-ground and below-ground pathogens. | plants modulate defence signalling networks in response to various biotic stresses via inter-organ communications. the root-mediated transmission of systemic acquired resistance (sar) against soil-borne and air-borne plant pathogens from sar-induced plants to neighbouring plants subjected to local chemical and pathogen treatments was evaluated. | 2016 | 27555496 |
| high quality draft genome sequence of the type strain of pseudomonas lutea ok2(t), a phosphate-solubilizing rhizospheric bacterium. | pseudomonas lutea ok2(t) (=lmg 21974(t), cect 5822(t)) is the type strain of the species and was isolated from the rhizosphere of grass growing in spain in 2003 based on its phosphate-solubilizing capacity. in order to identify the functional significance of phosphate solubilization in pseudomonas plant growth promoting rhizobacteria, we describe here the phenotypic characteristics of strain ok2(t) along with its high-quality draft genome sequence, its annotation, and analysis. the genome is com ... | 2016 | 27555890 |
| differential response of tomato genotypes to xanthomonas-specific pathogen-associated molecular patterns and correlation with bacterial spot (xanthomonas perforans) resistance. | plants depend on innate immune responses to retard the initial spread of pathogens entering through stomata, hydathodes or injuries. these responses are triggered by conserved patterns in pathogen-encoded molecules known as pathogen-associated molecular patterns (pamps). production of reactive oxygen species (ros) is one of the first responses, and the resulting 'oxidative burst' is considered to be a first line of defense. in this study, we conducted association analyses between ros production ... | 2016 | 27555919 |
| disruption of the cara gene in pseudomonas syringae results in reduced fitness and alters motility. | pseudomonas syringae infects diverse plant species and is widely used in the study of effector function and the molecular basis of disease. although the relationship between bacterial metabolism, nutrient acquisition and virulence has attracted increasing attention in bacterial pathology, there is limited knowledge regarding these studies in pseudomonas syringae. the aim of this study was to investigate the function of the cara gene and the small rna p32, and characterize the regulation of these ... | 2016 | 27558694 |
| the plant cell wall: a complex and dynamic structure as revealed by the responses of genes under stress conditions. | the plant cell wall has a diversity of functions. it provides a structural framework to support plant growth and acts as the first line of defense when the plant encounters pathogens. the cell wall must also retain some flexibility, such that when subjected to developmental, biotic, or abiotic stimuli it can be rapidly remodeled in response. genes encoding enzymes capable of synthesizing or hydrolyzing components of the plant cell wall show differential expression when subjected to different str ... | 2016 | 27559336 |
| comparative genomics of an endophytic pseudomonas putida isolated from mango orchard. | we analyzed the genome sequence of an endophytic bacterial strain pseudomonas putida tji51 isolated from mango bark tissues. next generation dna sequencing and short read de novo assembly generated the 5,805,096 bp draft genome of p. putida tji51. out of 6,036 protein coding genes in p. putida tji51 sequences, 4,367 (72%) were annotated with functional specifications, while the remaining encoded hypothetical proteins. comparative genome sequence analysis revealed that the p. putida tji51genome c ... | 2016 | 27560648 |
| lipid transfer proteins: classification, nomenclature, structure, and function. | the non-specific lipid transfer proteins (ltps) constitute a large protein family found in all land plants. they are small proteins characterized by a tunnel-like hydrophobic cavity, which makes them suitable for binding and transporting various lipids. the ltps are abundantly expressed in most tissues. in general, they are synthesized with an n-terminal signal peptide that localizes the protein to spaces exterior to the plasma membrane. the in vivo functions of ltps are still disputed, although ... | 2016 | 27562524 |
| epigenetic control of defense signaling and priming in plants. | immune recognition of pathogen-associated molecular patterns or effectors leads to defense activation at the pathogen challenged sites. this is followed by systemic defense activation at distant non-challenged sites, termed systemic acquired resistance (sar). these inducible defenses are accompanied by extensive transcriptional reprogramming of defense-related genes. sar is associated with priming, in which a subset of these genes is kept at a poised state to facilitate subsequent transcriptiona ... | 2016 | 27563304 |
| triticum monococcum lines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphid rhopalosiphum padi. | crop protection is an integral part of establishing food security, by protecting the yield potential of crops. cereal aphids cause yield losses by direct damage and transmission of viruses. some wild relatives of wheat show resistance to aphids but the mechanisms remain unresolved. in order to elucidate the location of the partial resistance to the bird cherry-oat aphid, rhopalosiphum padi, in diploid wheat lines of triticum monococcum, we conducted aphid performance studies using developmental ... | 2016 | 27570248 |
| phosphate uptake and allocation - a closer look at arabidopsis thaliana l. and oryza sativa l. | this year marks the 20th anniversary of the discovery and characterization of the two arabidopsis pht1 genes encoding the phosphate transporter in arabidopsis thaliana. so far, multiple inorganic phosphate (pi) transporters have been described, and the molecular basis of pi acquisition by plants has been well-characterized. these genes are involved in pi acquisition, allocation, and/or signal transduction. this review summarizes how pi is taken up by the roots and further distributed within two ... | 2016 | 27574525 |
| making healthier or killing enemies? bacterial volatile-elicited plant immunity plays major role upon protection of arabidopsis than the direct pathogen inhibition. | bacterial volatiles protect plants either by directly inhibiting a pathogenic fungus or by improving the defense capabilities of plants. the effect of bacterial volatiles on fungal growth was dose-dependent. a low dosage did not have a noticeable effect on botrytis cinerea growth and development, but was sufficient to elicit induced resistance in arabidopsis thaliana. bacterial volatiles displayed negative effects on biofilm formation on a polystyrene surface and in in planta leaf colonization o ... | 2016 | 27574539 |
| opposing control by transcription factors myb61 and myb3 increases freezing tolerance by relieving c-repeat binding factor suppression. | cold acclimation is an important process by which plants respond to low temperature and enhance their winter hardiness. c-repeat binding factor1 (cbf1), cbf2, and cbf3 genes were shown previously to participate in cold acclimation in medicago truncatula in addition, mtcbf4 is transcriptionally induced by salt, drought, and cold stresses. we show here that mtcbf4, shown previously to enhance drought and salt tolerance, also positively regulates cold acclimation and freezing tolerance. to identify ... | 2016 | 27578551 |
| high co2 primes plant biotic stress defences through redox-linked pathways. | industrial activities have caused tropospheric co2 concentrations to increase over the last two centuries, a trend that is predicted to continue for at least the next several decades. here, we report that growth of plants in a co2-enriched environment activates responses that are central to defense against pathogenic attack. salicylic acid accumulation was triggered by high-growth co2 in arabidopsis (arabidopsis thaliana) and other plants such as bean (phaseolus vulgaris). a detailed analysis in ... | 2016 | 27578552 |
| cold stress tolerance in psychrotolerant soil bacteria and their conferred chilling resistance in tomato (solanum lycopersicum mill.) under low temperatures. | the present work aimed to study the culturable diversity of psychrotolerant bacteria persistent in soil under overwintering conditions, evaluate their ability to sustain plant growth and alleviate chilling stress in tomato. psychrotolerant bacteria were isolated from agricultural field soil samples colleced during winter and then used to study chilling stress alleviation in tomato plants (solanum lycopersicum cv mill). selective isolation after enrichment at 5°c yielded 40 bacterial isolates. ph ... | 2016 | 27580055 |
| the structure of the periplasmic sensor domain of the histidine kinase cuss shows unusual metal ion coordination at the dimeric interface. | in bacteria, two-component systems act as signaling systems to respond to environmental stimuli. two-component systems generally consist of a sensor histidine kinase and a response regulator, which work together through histidyl-aspartyl phosphorelay to result in gene regulation. one of the two-component systems in escherichia coli, cuss-cusr, is known to induce expression of cuscfba genes at increased periplasmic cu(i) and ag(i) concentrations to help maintain metal ion homeostasis. cuss is a m ... | 2016 | 27583660 |
| an improved approach for measuring immersion freezing in large droplets over a wide temperature range. | immersion freezing (ice nucleation by particles immersed in supercooled water) is a key process for forming ice in mixed-phase clouds. immersion freezing experiments with particles in microliter-sized (millimeter-sized) water droplets are often applied to detecting very small numbers of ice nucleating particles (inps). however, the application of such large droplets remains confined to the detection of inps active at temperatures much higher than the homogeneous freezing limit, because of artifa ... | 2016 | 27596247 |
| spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol. | in order to predict the fate of chemicals in the environment, a range of regulatory tests are performed with microbial inocula collected from environmental compartments to investigate the potential for biodegradation. the abundance and distribution of microbes in the environment is affected by a range of variables, hence diversity and biomass of inocula used in biodegradation tests can be highly variable in space and time. the use of artificial or natural biofilms in regulatory tests could enabl ... | 2016 | 27596822 |
| enhanced tolerance of transgenic potato plants over-expressing non-specific lipid transfer protein-1 (stnsltp1) against multiple abiotic stresses. | abiotic stresses such as heat, drought, and salinity are major environmental constraints that limit potato (solanum tuberosum l.) production worldwide. previously, we found a potential thermo-tolerance gene, named stnsltp1 from potato using yeast functional screening. here, we report the functional characterization of stnsltp1 and its role in multiple abiotic stresses in potato plants. computational analysis of stnsltp1 with other plant ltps showed eight conserved cysteine residues, and four α-h ... | 2016 | 27597854 |
| screen of non-annotated small secreted proteins of pseudomonas syringae reveals a virulence factor that inhibits tomato immune proteases. | pseudomonas syringae pv. tomato dc3000 (ptodc3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. here we identified 131 candidate small, secreted, non-annotated proteins from the ptodc3000 genome, most of which are common to pseudomonas species and potentially expressed during apoplastic colonization. we produced 43 of these proteins through a custom-made gateway-compatible expression system for ... | 2016 | 27603016 |
| plasma membrane order and fluidity are diversely triggered by elicitors of plant defence. | although plants are exposed to a great number of pathogens, they usually defend themselves by triggering mechanisms able to limit disease development. alongside signalling events common to most such incompatible interactions, modifications of plasma membrane (pm) physical properties could be new players in the cell transduction cascade. different pairs of elicitors (cryptogein, oligogalacturonides, and flagellin) and plant cells (tobacco and arabidopsis) were used to address the issue of possibl ... | 2016 | 27604805 |
| involvement of type vi secretion system in secretion of iron chelator pyoverdine in pseudomonas taiwanensis. | rice bacterial blight caused by xanthomonas oryzae pv. oryzae (xoo) is one of the most destructive rice diseases worldwide. therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against xoo. here, we report that a soil bacterium pseudomonas taiwanensis displayed strong antagonistic activity against xoo. using matrix-assisted laser desorption/ionization imaging mass spectrometry, we identified an iron chelator, pyoverdine, secr ... | 2016 | 27605490 |
| environmental and genetic factors associated with solanesol accumulation in potato leaves. | solanesol is a high value 45-carbon, unsaturated, all-trans-nonaprenol isoprenoid. recently solanesol has received particular attention because of its utility, both in its own right and as a precursor in the production of numerous compounds used in the treatment of disease states. solanesol is found mainly in solanaceous crops such as potato, tomato, tobacco and pepper where it accumulates in the foliage. there is considerable potential to explore the extraction of solanesol from these sources a ... | 2016 | 27610114 |
| the diversity of mtdna rns introns among strains of ophiostoma piliferum, ophiostoma pluriannulatum and related species. | based on previous studies, it was suspected that the mitochondrial rns gene within the ophiostomatales is rich in introns. this study focused on a collection of strains representing ophiostoma piliferum, ophiostoma pluriannulatum and related species that cause blue-stain; these fungi colonize the sapwood of trees and impart a dark stain. this reduces the value of the lumber. the goal was to examine the mtdna rns intron landscape for these important blue stain fungi in order to facilitate future ... | 2016 | 27610327 |
| the proteasome acts as a hub for plant immunity and is targeted by pseudomonas type iii effectors. | recent evidence suggests that the ubiquitin-proteasome system is involved in several aspects of plant immunity and that a range of plant pathogens subvert the ubiquitin-proteasome system to enhance their virulence. here, we show that proteasome activity is strongly induced during basal defense in arabidopsis (arabidopsis thaliana). mutant lines of the proteasome subunits rpt2a and rpn12a support increased bacterial growth of virulent pseudomonas syringae pv tomato dc3000 (pst) and pseudomonas sy ... | 2016 | 27613851 |
| the arabidopsis miel1 e3 ligase negatively regulates aba signalling by promoting protein turnover of myb96. | the phytohormone abscisic acid (aba) regulates plant responses to various environmental challenges. controlled protein turnover is an important component of aba signalling. here we show that the ring-type e3 ligase myb30-interacting e3 ligase 1 (miel1) regulates aba sensitivity by promoting myb96 turnover in arabidopsis. germination of miel1-deficient mutant seeds is hypersensitive to aba, whereas miel1-overexpressing transgenic seeds are less sensitive. miel1 can interact with myb96, a regulato ... | 2016 | 27615387 |
| regulatory cross-talks and cascades in rice hormone biosynthesis pathways contribute to stress signaling. | crosstalk among different hormone signaling pathways play an important role in modulating plant response to both biotic and abiotic stress. hormone activity is controlled by its bio-availability, which is again influenced by its biosynthesis. thus, independent hormone biosynthesis pathways must be regulated and co-ordinated to mount an integrated response. one of the possibilities is to use cis-regulatory elements to orchestrate expression of hormone biosynthesis genes. analysis of cres, associa ... | 2016 | 27617021 |
| mitogen-activated protein kinase cascade mkk7-mpk6 plays important roles in plant development and regulates shoot branching by phosphorylating pin1 in arabidopsis. | emerging evidences exhibit that mitogen-activated protein kinase (mapk/mpk) signaling pathways are connected with many aspects of plant development. the complexity of mapk cascades raises challenges not only to identify the mapk module in planta but also to define the specific role of an individual module. so far, our knowledge of mapk signaling has been largely restricted to a small subset of mapk cascades. our previous study has characterized an arabidopsis bushy and dwarf1 (bud1) mutant, in w ... | 2016 | 27618482 |
| idl6-hae/hsl2 impacts pectin degradation and resistance to pseudomonas syringae pv tomato dc3000 in arabidopsis leaves. | plant cell walls undergo dynamic structural and chemical changes during plant development and growth. floral organ abscission and lateral root emergence are both accompanied by cell-wall remodeling, which involves the inflorescence deficient in abscission (ida)-derived peptide and its receptors, haesa (hae) and haesa-like2 (hsl2). plant cell walls also act as barriers against pathogenic invaders. thus, the cell-wall remodeling during plant development could have an influence on plant resistance ... | 2017 | 27618493 |
| the multivesicular bodies (mvbs)-localized aaa atpase lrd6-6 inhibits immunity and cell death likely through regulating mvbs-mediated vesicular trafficking in rice. | previous studies have shown that multivesicular bodies (mvbs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. however, the molecular regulation is poorly understood in rice. here we report the identification and characterization of a mvbs-localized aaa atpase lrd6-6 in rice. disruption of lrd6-6 leads to enhanced immunity and cell death in rice. the atpase activity and homo-dimerization of lrd6-6 is essential for its regulation on plant immunity and ... | 2016 | 27618555 |
| nad acts as an integral regulator of multiple defense layers. | pyridine nucleotides, such as nad, are crucial redox carriers and have emerged as important signaling molecules in stress responses. previously, we have demonstrated in arabidopsis (arabidopsis thaliana) that the inducible nad-overproducing nadc lines are more resistant to an avirulent strain of pseudomonas syringae pv tomato (pst-avrrpm1), which was associated with salicylic acid-dependent defense. here, we have further characterized the nad-dependent immune response in arabidopsis. quinolinate ... | 2016 | 27621425 |
| sensitivity of pseudomonas syringae to bovine lactoferrin hydrolysates and identification of a novel inhibitory peptide. | the antimicrobial activity of bovine lactoferrin hydrolysates (blfh) was measured against pseudomonas strains (p. syringae and p. fluorescens) in vitro. to compare susceptibility to blfh, minimal inhibitory concentration (mic) values were determined using chemiluminescence assays and paper disc plate assays. antimicrobial effect against p. fluorescens was not observed by either assay, suggesting that blfh did not exhibit antimicrobial activity against p. fluorescens. however, a significant inhib ... | 2016 | 27621689 |
| bioprospecting red sea coastal ecosystems for culturable microorganisms and their antimicrobial potential. | microorganisms that inhabit unchartered unique soil such as in the highly saline and hot red sea lagoons on the saudi arabian coastline, represent untapped sources of potentially new bioactive compounds. in this study, a culture-dependent approach was applied to three types of sediments: mangrove mud (mn), microbial mat (mm), and barren soil (bs), collected from rabigh harbor lagoon (rhl) and al-kharrar lagoon (akl). the isolated bacteria were evaluated for their potential to produce bioactive c ... | 2016 | 27626430 |
| allocation of heme is differentially regulated by ferrochelatase isoforms in arabidopsis cells. | heme is involved in various biological processes as a cofactor of hemoproteins located in various organelles. in plant cells, heme is synthesized by two isoforms of plastid-localized ferrochelatase, fc1 and fc2. in this study, by characterizing arabidopsis t-dna insertional mutants, we showed that the allocation of heme is differentially regulated by ferrochelatase isoforms in plant cells. analyses of weak (fc1-1) and null (fc1-2) mutants suggest that fc1-producing heme is required for initial g ... | 2016 | 27630653 |
| persistent supercooling of reproductive shoots is enabled by structural ice barriers being active despite an intact xylem connection. | extracellular ice nucleation usually occurs at mild subzero temperatures in most plants. for persistent supercooling of certain plant parts ice barriers are necessary to prevent the entry of ice from already frozen tissues. the reproductive shoot of calluna vulgaris is able to supercool down to below -22°c throughout all developmental stages (shoot elongation, flowering, fruiting) despite an established xylem conductivity. after localization of the persistent ice barrier between the reproductive ... | 2016 | 27632365 |
| transcription factor anac032 modulates ja/sa signalling in response to pseudomonas syringae infection. | responses to pathogens, including host transcriptional reprogramming, require partially antagonistic signalling pathways dependent on the phytohormones salicylic (sa) and jasmonic (ja) acids. however, upstream factors modulating the interplay of these pathways are not well characterized. here, we identify the transcription factor anac032 from arabidopsis thaliana as one such regulator in response to the bacterial pathogen pseudomonas syringae pv. tomato dc3000 (pst). anac032 directly represses m ... | 2016 | 27632992 |
| indole-3-acetic acid in plant-pathogen interactions: a key molecule for in planta bacterial virulence and fitness. | the plant pathogenic bacterium pseudomonas savastanoi, the causal agent of olive and oleander knot disease, uses the so-called "indole-3-acetamide pathway" to convert tryptophan to indole-3-acetic acid (iaa) via a two-step pathway catalyzed by enzymes encoded by the genes in the iaam/iaah operon. moreover, pathovar nerii of p. savastanoi is able to conjugate iaa to lysine to generate the less biologically active compound iaa-lys via the enzyme iaa-lysine synthase encoded by the iaal gene. intere ... | 2017 | 27637152 |
| allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in arabidopsis thaliana. | isothiocyanates (itcs) are degradation products of the plant secondary metabolites glucosinolates (gsls) and are known to affect human health as well as plant herbivores and pathogens. to investigate the processes engaged in plants upon exposure to isothiocyanate we performed a genome scale transcriptional profiling of arabidopsis thaliana at different time points in response to an exogenous treatment with allyl-isothiocyanate. | 2016 | 27639974 |
| q&a: how does jasmonate signaling enable plants to adapt and survive? | jasmonates (jas) are a class of plant hormones that play essential roles in response to tissue wounding. they act on gene expression to slow down growth and to redirect metabolism towards producing defense molecules and repairing damage. these responses are systemic and have dramatic impacts on yields, making jas a very active research area. jas interact with many other plant hormones and therefore also have essential functions throughout development, notably during plant reproduction, leaf sene ... | 2016 | 27643853 |
| calecrk-s.5, a pepper l-type lectin receptor kinase gene, confers broad-spectrum resistance by activating priming. | in arabidopsis, several l-type lectin receptor kinases (lecrks) have been identified as putative immune receptors. however, to date, there have been few analyses of lecrks in crop plants. virus-induced gene silencing of calecrk-s.5 verified the role of calecrk-s.5 in broad-spectrum resistance. compared with control plants, calecrk-s.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation ... | 2016 | 27647723 |
| identification of reference genes for rt-qpcr data normalization in cannabis sativa stem tissues. | gene expression profiling via quantitative real-time pcr is a robust technique widely used in the life sciences to compare gene expression patterns in, e.g., different tissues, growth conditions, or after specific treatments. in the field of plant science, real-time pcr is the gold standard to study the dynamics of gene expression and is used to validate the results generated with high throughput techniques, e.g., rna-seq. an accurate relative quantification of gene expression relies on the iden ... | 2016 | 27649158 |
| genome-wide identification and characterization of wrky gene family in salix suchowensis. | wrky proteins are the zinc finger transcription factors that were first identified in plants. they can specifically interact with the w-box, which can be found in the promoter region of a large number of plant target genes, to regulate the expressions of downstream target genes. they also participate in diverse physiological and growing processes in plants. prior to this study, a plenty of wrky genes have been identified and characterized in herbaceous species, but there is no large-scale study ... | 2016 | 27651997 |
| aboveground whitefly infestation-mediated reshaping of the root microbiota. | plants respond to various types of herbivore and pathogen attack using well-developed defensive machinery designed for self-protection. infestation from phloem-sucking insects such as whitefly and aphid on plant leaves was previously shown to influence both the saprophytic and pathogenic bacterial community in the plant rhizosphere. however, the modulation of the root microbial community by plants following insect infestation has been largely unexplored. only limited studies of culture-dependent ... | 2016 | 27656163 |
| stress-inducible expression of an f-box gene tafba1 from wheat enhanced the drought tolerance in transgenic tobacco plants without impacting growth and development. | e3 ligase plays an important role in the response to many environment stresses in plants. in our previous study, constitutive overexpression of an f-box protein gene tafba1 driven by 35s promoter improved the drought tolerance in transgenic tobacco plants, but the growth and development in transgenic plants was altered in normal conditions. in this study, we used stress-inducible promoter rd29a instead of 35s promoter, as a results, the stress-inducible transgenic tobacco plants exhibit a simila ... | 2016 | 27656187 |
| the lipopolysaccharide-induced metabolome signature in arabidopsis thaliana reveals dynamic reprogramming of phytoalexin and phytoanticipin pathways. | lipopolysaccharides (lpss), as mamp molecules, trigger the activation of signal transduction pathways involved in defence. currently, plant metabolomics is providing new dimensions into understanding the intracellular adaptive responses to external stimuli. the effect of lps on the metabolomes of arabidopsis thaliana cells and leaf tissue was investigated over a 24 h period. cellular metabolites and those secreted into the medium were extracted with methanol and liquid chromatography coupled to ... | 2016 | 27656890 |
| lon protease is involved in rhprs-mediated regulation of type iii secretion in pseudomonas syringae. | pseudomonas syringae depends on the type iii secretion system (t3ss) to directly translocate effectors into host cells. previously, we reported a nonpathogenic rhps mutant, suggesting that the two-component transduction system rhprs is an important regulator of t3ss in p. syringae. rhprs regulates itself and a variety of downstream genes under an inverted repeat element promoter in a phosphorylation-dependent manner. here, we identify lon as a suppressor of the rhps mutant through transposon scr ... | 2016 | 27657922 |
| expression of cucumber mosaic virus suppressor 2b alters fwa methylation and its sirna accumulation in arabidopsis thaliana. | the cucumber mosaic virus (cmv) suppressor 2b co-localizes with ago4 in cytoplasmic and nuclear fractions of arabidopsis thaliana biochemical fractionation of a. thaliana cellular extracts revealed that 2b and ago4 coexist in multiple size exclusions. 2b transgenic a. thaliana exhibited an enhanced accumulation of 24nt sirnas from flowering wageningen (fwa) and other heterochromatic loci. these plants also exhibited hypo-methylation of an endogenous- as well as transgene-fwa promoter at non-cg s ... | 2016 | 27659688 |
| draft genome sequence of a diazotrophic, plant growth-promoting rhizobacterium of the pseudomonas syringae complex. | we report here the draft genome sequence of pseudomonas syringae gr12-2, a nitrogen-fixing, plant growth-promoting bacterium, isolated from the rhizosphere of an arctic grass. the 6.6-mbp genome contains 5,676 protein-coding genes, including a nitrogen-fixation island similar to that in p. stutzeri. | 2016 | 27660794 |
| differential rickettsial transcription in bloodfeeding and non-bloodfeeding arthropod hosts. | crucial factors influencing the epidemiology of rickettsia felis rickettsiosis include pathogenesis and transmission. detection of r. felis dna in a number of arthropod species has been reported, with characterized isolates, r. felis strain lsu and strain lsu-lb, generated from the cat flea, ctenocephalides felis, and the non-hematophagous booklouse, liposcelis bostrychophila, respectively. while it is realized that strain influence on host biology varies, the rickettsial response to these disti ... | 2016 | 27662479 |
| the bacteriophage-derived transcriptional regulator, lscr, activates the expression of levansucrase genes in pseudomonas syringae. | synthesis of the exopolysaccharide levan occurs in the bacterial blight pathogen of soybean, pseudomonas syringae pv. glycinea pg4180, when this bacterium encounters moderate to high concentrations of sucrose inside its host plant. the process is mediated by the temperature-dependent expression and secretion of two levansucrases, lscb and lscc. previous studies showed the importance of a prophage-associated promoter element in driving the expression of levansucrase genes. herein, heterologous sc ... | 2016 | 27664099 |
| effector diversification contributes to xanthomonas oryzae pv. oryzae phenotypic adaptation in a semi-isolated environment. | understanding the processes that shaped contemporary pathogen populations in agricultural landscapes is quite important to define appropriate management strategies and to support crop improvement efforts. here, we took advantage of an historical record to examine the adaptation pathway of the rice pathogen xanthomonas oryzae pv. oryzae (xoo) in a semi-isolated environment represented in the philippine archipelago. by comparing genomes of key xoo groups we showed that modern populations derived f ... | 2016 | 27667260 |
| a novel mutant allele of ssi2 confers a better balance between disease resistance and plant growth inhibition on arabidopsis thaliana. | resistance and growth are opposing characteristics in plants. sa insensitivity of npr1-5 (ssi2) encodes a stearoyl-acp desaturase (s-acp des) that has previously been reported to simultaneously enhance resistance and repress growth. | 2016 | 27669891 |
| modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of cyclin-dependent kinase a;1. | modulation of protein activity by phosphorylation through kinases and subsequent de-phosphorylation by phosphatases is one of the most prominent cellular control mechanisms. thus, identification of kinase substrates is pivotal for the understanding of many - if not all - molecular biological processes. equally, the possibility to deliberately tune kinase activity is of great value to analyze the biological process controlled by a particular kinase. | 2016 | 27669979 |
| uv-b radiation stress causes alterations in whole cell protein profile and expression of certain genes in the rice phyllospheric bacterium enterobacter cloacae. | among the different types of uv radiation, uv-b radiation (280-315 nm) has gained much attention mainly due to its increasing incidence on the earth's surface leading to imbalances in natural ecosystems. this study deals with the effects of uv-b radiation on the proteome and gene expression in a rice phyllospheric bacterium, enterobacter cloacae. of the five bacteria isolated from rice leaves, e. cloacae showed the highest level of resistance to uv-b and total killing occurred after 8 h of conti ... | 2016 | 27672388 |
| a small cysteine-rich protein from the asian soybean rust fungus, phakopsora pachyrhizi, suppresses plant immunity. | the asian soybean rust fungus, phakopsora pachyrhizi, is an obligate biotrophic pathogen causing severe soybean disease epidemics. molecular mechanisms by which p. pachyrhizi and other rust fungi interact with their host plants are poorly understood. the genomes of all rust fungi encode many small, secreted cysteine-rich proteins (sscrp). while these proteins are thought to function within the host, their roles are completely unknown. here, we present the characterization of p. pachyrhizi effect ... | 2016 | 27676173 |
| the bacterial signature of leptospermum scoparium (mānuka) reveals core and accessory communities with bioactive properties. | leptospermum scoparium or mānuka is a new zealand native medicinal plant that produces an essential oil with antimicrobial properties. this is the first study to investigate the structure and bioactivity of endophytic bacteria in mānuka by using a combination of cultivation-independent (dgge) and dependent approaches. a total of 23 plants were sampled across three sites. plants were considered either immature (3-8 years) or mature (>20 years). the endophyte community structure and richness was a ... | 2016 | 27676607 |