Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| listeria-infected myeloid dendritic cells produce ifn-beta, priming t cell activation. | the intracellular bacterium listeria monocytogenes infects dendritic cells (dc) and other apcs and induces potent cell-mediated protective immunity. however, heat-killed bacteria fail to do so. this study explored whether dc differentially respond to live and killed listeria and how this affects t cell activation. to control for bacterial number, a replication-deficient strain, lmdd, defective in d-alanine biosynthesis, was used. we found that dc internalize both live and heat-killed lmdd and si ... | 2005 | 15972676 |
| evolution of listeria populations in food samples undergoing enrichment culturing. | the isolation of listeria monocytogenes from food is carried out using a double enrichment. it is believed that the double enrichment can allow the overgrowth of listeria innocua in samples where both species are present. in this study, we have evaluated the impact of overgrowth between listeria species and strains during each step of the enrichment process. the effect of factors minimizing interactions between strains or phage inhibitory effects has also been estimated. in an artificially conta ... | 2005 | 15975676 |
| autistic effector t cells in mice with a point mutation in the lat adaptor fail to respond to listeria monocytogenes infection. | the adaptor protein linker for activation of t cells (lat) is an important transducer of extracellular t cell stimuli. in mice with a point mutation in lat (laty136f), tcr signaling is substantially compromised and laty136f t cells are unresponsive to cd3 cross-linking in vitro. nevertheless, laty136f mice develop a polyclonal lymphoproliferation of cd4(+) t cells, which display a t(h)2-polarized effector phenotype. in this study, laty136f mice were infected with the intracellular bacterium list ... | 2005 | 15976034 |
| prediction of pathogen growth on iceberg lettuce under real temperature history during distribution from farm to table. | the growth of pathogenic bacteria escherichia coli o157:h7, salmonella spp., and listeria monocytogenes on iceberg lettuce under constant and fluctuating temperatures was modelled in order to estimate the microbial safety of this vegetable during distribution from the farm to the table. firstly, we examined pathogen growth on lettuce at constant temperatures, ranging from 5 to 25 degrees c, and then we obtained the growth kinetic parameters (lag time, maximum growth rate (micro(max)), and maximu ... | 2005 | 15979180 |
| an outbreak of food-borne listeriosis due to cheese in japan, during 2001. | food-borne outbreaks caused by listeria monocytogenes have been recognized in us and european countries. only sporadic cases, of neonatal listeriosis, have been reported in japan. since l. monocytogenes has been often isolated from foods in japan, food-borne outbreaks potentially could have occurred. in february 2001, l. monocytogenes serotype 1/2b was isolated from a washed-type cheese during routine listeria monitoring of 123 domestic cheeses. further samples from products and the environments ... | 2005 | 15979181 |
| effect of inoculation of carnobacterium divergens v41, a bio-preservative strain against listeria monocytogenes risk, on the microbiological, chemical and sensory quality of cold-smoked salmon. | the aim of this study was to develop a bio-preservation strategy for cold-smoked salmon (css) by the use of lactic acid bacteria previously selected for their capability to inhibit the growth of listeria monocytogenes in the product. the spoiling potential of three carnobacterium strains (carnobacterium divergens v41, carnobacterium piscicola v1 and sf668) was tested in sterile css blocks inoculated by 10(4-5) cfu g(-)(1) and stored under vacuum for 9 days at 4 degrees c followed by 19 days at 8 ... | 2005 | 15979753 |
| bacterial shape and acta distribution affect initiation of listeria monocytogenes actin-based motility. | we have examined the process by which the intracellular bacterial pathogen listeria monocytogenes initiates actin-based motility and determined the contribution of the variable surface distribution of the acta protein to initiation and steady-state movement. to directly correlate acta distributions to actin dynamics and motility of live bacteria, acta was fused to a monomeric red fluorescent protein (mrfp1). actin comet tail formation and steady-state bacterial movement rates both depended on ac ... | 2005 | 15980176 |
| bioluminescent imaging of melanoma in live mice. | melanoma is highly resistant to conventional chemotherapeutic agents and novel therapeutic approaches are needed. current animal models of melanoma in animals are sub-optimal. the most commonly used homograft model is the b16 mouse melanoma. evaluation of potential melanoma therapies with this model is limited by the inaccuracy of caliper measurement of subcutaneous tumors, of counting lung nodules in metastasis models, and the indirect nature of "survival" curves when studying brain metastases. ... | 2005 | 15982316 |
| production of antibodies to canine il-1beta and canine tnf to assess the role of proinflammatory cytokines. | il-1 and tnf are important proinflammatory cytokines implicated in both antimicrobial host defense and pathogenesis of diseases with an immune-mediated and/or inflammatory component. respective studies in the dog have been hampered by the unavailability of reagents allowing the specific measurement of canine cytokine proteins and the effect of canine cytokine neutralization by ab. starting with recombinant canine (rcan) il-1beta and rcantnf, four polyclonal antisera and 22 mab specific for rcani ... | 2005 | 15982477 |
| tumor necrosis factor and its blockade in granulomatous infections: differential modes of action of infliximab and etanercept? | tumor necrosis factor (tnf) is a critical component of both the antibacterially protective and the inflammatory responses against infections, particularly infections with intracellularly viable microorganisms. it is, therefore, not surprising that some treatment regimens that target tnf function have resulted in an increase in complications associated with infections due to such pathogens as mycobacterium tuberculosis, listeria monocytogenes, and histoplasma capsulatum; organized granuloma forma ... | 2005 | 15983900 |
| the host type i interferon response to viral and bacterial infections. | type i interferons (ifn) are well studied cytokines with anti-viral and immune-modulating functions. type i ifns are produced following viral infections, but until recently, the mechanisms of viral recognition leading to ifn production were largely unknown. toll like receptors (tlrs) have emerged as key transducers of type i ifn during viral infections by recognizing various viral components. furthermore, much progress has been made in defining the signaling pathways downstream of tlrs for type ... | 2005 | 15987599 |
| anti-listeria monocytogenes bacteriocin-like inhibitory substances from enterococcus faecium uq31 isolated from artisan mexican-style cheese. | artisan fresh mexican-style cheeses are commonly made from raw milk that provides not only rich flavors, but also a diversity of associated lactic acid bacteria (lab) strains. enterococcus faecium uq31 was isolated from panela cheese and produced bacteriocin-like inhibitory substances (blis) with a strong anti-listeria activity. a modified ph-mediated adsorption-desorption purification process resulted in (after sds-page) two bands showing antimicrobial activities, where most of the activity cor ... | 2005 | 15991053 |
| evaluation of universal pre-enrichment broth for isolation of salmonella spp., escherichia coli o157:h7, and listeria monocytogenes from dairy farm environmental samples. | use of universal pre-enrichment broth (upb) as a primary enrichment medium for detection of salmonella spp., escherichia coli o157:h7, and listeria monocytogenes from dairy farm environmental samples was evaluated. there were no differences in bacterial growth between upb and selective primary enrichment broths for each pathogen inoculated individually or in combination at 10(1) and 10(2) colony forming units/ml. in addition, no differences were observed when upb and selective primary enrichment ... | 2004 | 15992260 |
| detection of sorbitol-negative and sorbitol-positive shiga toxin-producing escherichia coli, listeria monocytogenes, campylobacter jejuni, and salmonella spp. in dairy farm environmental samples. | six visits were conducted to four dairy farms to collect swab, liquid, and solid dairy farm environmental samples (165 to 180/farm; 15 sample types). the objective of the study was to determine on-farm sources of campylobacter jejuni, salmonella spp., listeria monocytogenes, and shiga toxin-producing escherichia coli (stec), which might serve as reservoirs for transmission of pathogens. samples were analyzed using mostly u.s. food and drug administration's bacteriological analytical manual proto ... | 2004 | 15992268 |
| causes behind a human cheese-borne outbreak of gastrointestinal listeriosis. | in a previous paper, we reported an outbreak of gastrointestinal listeriosis due to consumption of fresh cheese made from raw milk and manufactured on a summer farm. the aim of the present study was to investigate why the cheese harbored listeria monocytogenes. to our knowledge, this is the first documented outbreak of listeriosis caused by raw milk cheese where the human epidemic strain has been cultured from a dairy animal, whose milk has been used for cheese production. the conditions on a su ... | 2004 | 15992274 |
| accelerating bacterial identification by infrared spectroscopy by employing microarray deposition of microorganisms. | a microarray method for the deposition of bacteria onto an agar slide was developed to accelerate the formation of microcolonies. representative microarrays each consisting of 40 micro-spots of five replicates of eight foodborne bacteria (yersinia enterocolitica, staphylococcus aureus, salmonella typhimurium, listeria monocytogenes, enterobacter cloacae, citrobacter freundii, klebsiella pneumoniae, and escherichia coli) were printed on a brain heart infusion (bhi) agar slide using a contact micr ... | 2004 | 15992277 |
| detection of escherichia coli o157:h7 and listeria monocytogenes in beef products by real-time polymerase chain reaction. | rapid methods for the detection of escherichia coli o157:h7 and listeria monocytogenes in food products are important to the food industry and for public health. conventional microbiological methods and newly developed molecular-based techniques such as polymerase chain reaction (pcr)-based methods are time consuming. in this study, a faster method based on utilization of a hybridization probe with real-time pcr, was developed and applied for detection of e. coli o157:h7 and l. monocytogenes fro ... | 2004 | 15992285 |
| foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. | milk and products derived from milk of dairy cows can harbor a variety of microorganisms and can be important sources of foodborne pathogens. the presence of foodborne pathogens in milk is due to direct contact with contaminated sources in the dairy farm environment and to excretion from the udder of an infected animal. most milk is pasteurized, so why should the dairy industry be concerned about the microbial quality of bulk tank milk? there are several valid reasons, including (1) outbreaks of ... | 2005 | 15992306 |
| effect of the implementation of haccp on the microbiological quality of meals at a university restaurant. | a study was conducted to evaluate the microbiological quality, including total mesophilic counts and markers of bacteriological hygiene, as indicator of food safety of three categories of the most consumed meals in a university restaurant, before and after implementation of the haccp system and personnel training. cold gastronomy products, cooked warm-served products, and cooked cold-served products were tested for bacterial contamination. throughout the experiment, 894 samples were examined for ... | 2005 | 15992308 |
| antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin. | antibiotic resistance and antibacterial activity were determined on heterotrophic bacteria isolated from mineral waters. of the 120 isolates pseudomonas spp. (55.8%) was the predominant group followed by acinetobacter spp. (14.17%), flavobacterium spp. (10.83%), achromobacter spp. (10%), burkholderia cepacia (3.3%), agrobacterium/radiobacter (2.5%), moraxella spp. (1.7%), aeromonas hydrophila (1.7%). over 80% of the isolates were resistant to one or more antibiotics and the highest resistance wa ... | 2005 | 15993695 |
| prevalence and location of listeria monocytogenes in farmed rainbow trout. | a total of 510 rainbow trout originating from fish farms in lakes and sea areas around finland were studied for the presence of listeria monocytogenes. samples were studied as pools from five fish. gill, viscera, and skin from the pooled samples were analysed separately. the individual samples were analysed later if the pooled sample was found to be listeria positive. the prevalence of listeria spp. and l. monocytogenes in pooled unprocessed fresh rainbow trout was on average 35.0% and 14.6%, re ... | 2005 | 15993967 |
| characterization of listeria monocytogenes isolates from food animal clinical cases: pfge pattern similarity to strains from human listeriosis cases. | twenty-one isolates of listeria monocytogenes from food animal clinical cases that involved meningitis or meningoencephalitis, encephalitis, mastitis and abortion were characterized by serotyping and pulsed-field gel electrophoresis (pfge) in order to improve our understanding of the genetic links between individual strains and strains recovered from human listeriosis cases. results showed that five of the isolates were serotype 1/2a, six were 1/2b, nine were 4b, and one was untypeable. a caprin ... | 2005 | 16000241 |
| suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of listeria monocytogenes from genomic dna. | listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. suspension microarray technology eliminates the technical issues associated with planar micr ... | 2005 | 16000444 |
| definition of genetically distinct attenuation mechanisms in naturally virulence-attenuated listeria monocytogenes by comparative cell culture and molecular characterization. | listeria monocytogenes is a foodborne pathogen able to cause serious disease in humans and animals. not all isolates are equally pathogenic, however, and several isolates have been characterized as naturally virulence attenuated. we sought to identify the genetic basis of natural virulence attenuation using cell culture assays and molecular techniques. by comparing the phenotypes of naturally virulence-attenuated isolates to those of defined virulence gene mutants in plaque, cytotoxicity, and he ... | 2005 | 16000803 |
| functional transfer of eukaryotic expression plasmids to mammalian cells by listeria monocytogenes: a mechanistic approach. | cystic fibrosis (cf) is one of the most common monogenic disorders in the caucasian population. gene therapy for cf is principally feasible and bacterial transfer systems might provide novel possibilities for therapy. however, transfection efficiencies are low and need to be improved. thus, more detailed understanding of the dna transfer mechanism is necessary to systematically eliminate these restrictions. | 2005 | 16001391 |
| experimental evaluation of different precision criteria applicable to microbiological counting methods. | the dispersion of microbiological counting measurements, when repeating the analysis on the same material both within a laboratory (repeatability) and between laboratories (reproducibility) can be characterized by the organization of interlaboratory studies, where several sets of identical test materials are sent to several laboratories. using the example of data generated by an interlaboratory study on enumeration of listeria monocytogenes in foods by the standardized reference method (colony-c ... | 2005 | 16001859 |
| cpg oligonucleotides partially inhibit growth of mycobacterium tuberculosis, but not salmonella or listeria, in human monocyte-derived macrophages. | immunostimulatory dna sequences and their synthetic oligonucleotide analogs (cpg-odn) activate innate immunity and can stimulate antibacterial effects against numerous intracellular pathogens. while it has been shown previously that cpg-odn inhibit growth of mycobacterium avium in murine and human macrophages, we now report that mycobacterium tuberculosis growth can be inhibited by cpg-odn treatment of human monocyte-derived macrophages (hmdm). this inhibitory effect was reversed by ifn-gamma, w ... | 2005 | 16002269 |
| hepatocytes express abundant surface class i mhc and efficiently use transporter associated with antigen processing, tapasin, and low molecular weight polypeptide proteasome subunit components of antigen processing and presentation pathway. | hepatic expression levels of class i mhc ags are generally regarded as very low. because the status of these ags and their ability to present peptides are important for the understanding of pathogen clearance and tolerogenic properties of the liver, we set out to identify the factors contributing to the reported phenotype. unexpectedly, we found that the surface densities of k(b) and d(b) on c57bl/6 mouse hepatocytes are nearly as high as on splenocytes, as are the lysate concentrations of mrna ... | 2005 | 16002705 |
| invariant v alpha 14+ nkt cells participate in the early response to enteric listeria monocytogenes infection. | invariant valpha14(+) nkt cells are a specialized cd1-reactive t cell subset implicated in innate and adaptive immunity. we assessed whether valpha14(+) nkt cells participated in the immune response against enteric listeria monocytogenes infection in vivo. using cd1d tetramers loaded with the synthetic lipid alpha-galactosylceramide (cd1d/alphagc), we found that splenic and hepatic valpha14(+) nkt cells in c57bl/6 mice were early producers of ifn-gamma (but not il-4) after l. monocytogenes infec ... | 2005 | 16002715 |
| effect of inoculum size on the combined temperature, ph and aw limits for growth of listeria monocytogenes. | the growth/no growth responses of listeria monocytogenes inoculated at four levels (0.90, 2.58, 4.20 and 6.81 log cfu/well; 300 microl medium/well) into tryptic soy broth (tsb) were monitored at different combinations of temperature (4 to 30 degrees c), ph (3.76 to 6.44) and aw (0.888 to 0.997) for 60 days. the study was conducted in 96-well microtiter plates and growth was monitored visually and by recording the turbidity of the medium with an automated microplate reader. the growth limits of t ... | 2005 | 16005535 |
| treatment of brain abscess due to listeria monocytogenes. | 2005 | 16007552 | |
| t cell reactivity to listeria monocytogenes amongst us. | in the last two decades, listeriosis, caused by the intracellular pathogen listeria monocytogenes, became one of the most concerning food-born infections. although it had been known before as an infectious disease of limited importance, listeriosis was brought into attention of scientists due to the frequent outbreaks recently reported. despite the major progress made towards understanding the mechanisms of virulence of l. monocytogenes, our current knowledge into the process of listeria-associa ... | 2003 | 16008146 |
| a novel role for the lisrk two-component regulatory system in listerial osmotolerance. | understanding how pathogenic bacteria sense and respond to environmental signals, including those involved in triggering virulence gene expression, is a fundamental biological goal. it is now known that the two-component regulatory system lisrk has a novel role in osmosensing and osmoregulation in the intracellular foodborne pathogen listeria monocytogenes. furthermore, htra, a gene linked to osmotolerance and virulence potential in l. monocytogenes, is now known to be under the transcriptional ... | 2005 | 16008610 |
| reflections on the use of robust and least-squares non-linear regression to model challenge tests conducted in/on food products. | in this research, we question the straight-forward use of the classical sum of squared error criterion for identifying the typical parameters of a primary model (like growth rate mumax and lag time lambda) when applied to growth curves obtained in and on food products. firstly, we base our reflections on 62 listeria monocytogenes laboratory challenge tests collected in various environments (broth, crushed cold-smoked salmon, and surface of cold-smoked salmon slices). whereas growth data in broth ... | 2005 | 16009440 |
| [spontaneous bacterial peritonitis caused by listeria monocytogenes. review of one case]. | 2005 | 16013103 | |
| stability and antimicrobial efficiency of eugenol encapsulated in surfactant micelles as affected by temperature and ph. | growth inhibition of four strains of escherichia coli o157:h7 (h1730, f4546, 932, and e0019) and listeria monocytogenes (scott a, 101, 108, and 310) by eugenol encapsulated in water soluble micellar nonionic surfactant solutions (surfynol 485w) adjusted to ph 5, 6, and 7 and incubated at 10, 22, and 32 degrees c was determined. concentrations of eugenol ranged from 0.2 to 0.9% at a surfactant concentration of 5%. antimicrobial activity was assessed using a microbroth dilution assay. eugenol enca ... | 2005 | 16013371 |
| survival of listeria monocytogenes and escherichia coli o157:h7 during sauerkraut fermentation. | sauerkraut was produced from shredded cabbage, as is typical in the united states, and from whole head cabbages, which is a traditional process in parts of eastern europe. the sauerkraut was inoculated with five strain mixtures of escherichia coli o157:h7 and listeria monocytogenes, and the populations of these bacteria, as well as lactic acid bacteria, ph, and titratable acidity, were monitored over the course of fermentation. fermentation variables were temperature (18 and 22 degrees c) and sa ... | 2005 | 16013372 |
| enhancing the bactericidal effect of electrolyzed water on listeria monocytogenes biofilms formed on stainless steel. | biofilms are potential sources of contamination to food in processing plants, because they frequently survive sanitizer treatments during cleaning. the objective of this research was to investigate the combined use of alkaline and acidic electrolyzed (eo) water in the inactivation of listeria monocytogenes biofilms on stainless steel surfaces. biofilms were grown on rectangular stainless steel (type 304, no. 4 finish) coupons (2 by 5 cm) in a 1:10 dilution of tryptic soy broth that contained a f ... | 2005 | 16013373 |
| combined efficacy of nisin and pediocin with sodium lactate, citric acid, phytic acid, and potassium sorbate and edta in reducing the listeria monocytogenes population of inoculated fresh-cut produce. | the inability of chlorine to completely inactivate human bacterial pathogens on whole and fresh-cut produce suggests a need for other antimicrobial washing treatments. nisin (50 microg/ml) and pediocin (100 au/ml) individually or in combination with sodium lactate (2%), potassium sorbate (0.02%), phytic acid (0.02%), and citric acid (10 mm) were tested as possible sanitizer treatments for reducing the population of listeria monocytogenes on cabbage, broccoli, and mung bean sprouts. cabbage, broc ... | 2005 | 16013374 |
| food safety knowledge of consumers and the microbiological and temperature status of their refrigerators. | the objectives of this study were to examine domestic food safety knowledge levels of consumers, establish the levels and incidence of bacterial contamination and operational temperatures in domestic refrigerators, and identify areas in which consumer food safety education is necessary in ireland. a food safety knowledge questionnaire applied to a representative sample of households (n = 1,020) throughout the island of ireland found the gaps in consumer food safety knowledge. analysis of swab sa ... | 2005 | 16013380 |
| microbicidal activity of tripotassium phosphate and fatty acids toward spoilage and pathogenic bacteria associated with poultry. | the ability of solutions of tripotassium phosphate (tpp) and fatty acids (lauric and myristic acids) to reduce populations of spoilage and pathogenic microorganisms associated with processed poultry was examined. in vitro studies were conducted with cultures of bacteria (campylobacter jejuni, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, and staphylococcus aureus) and yeasts (candida ernobii and yarrowia lipolytica). cultures of the bacteria and yeasts ... | 2005 | 16013388 |
| rapid quantitative detection of, listeria monocytogenes in salmon products: evaluation of pre-real-time pcr strategies. | the spread and persistence of listeria monocytogenes in smoked fish products and seafood processing factories are big concerns. thus, the corresponding quality assurance programs must include adequate microbiological control measures. we evaluated eight different pre-pcr sample processing strategies to be coupled with a previously developed real-time pcr assay for the quantitative detection of l. monocytogenes in salmon products. the optimal pre-pcr procedure involved filtration and dna purifica ... | 2005 | 16013389 |
| effect of ph on the inhibition of listeria spp. by vanillin and vanillic acid. | the antimicrobial effects of vanillin and vanillic acid were verified against several species and strains of listeria monocytogenes, listeria innocua, listeria grayi, and listeria seeligeri in a laboratory medium adjusted to ph values ranging from 5.0 to 8.0. medium ph had little influence on the mic of vanillin as determined by a broth dilution assay, and growth of all test strains was inhibited by concentrations ranging from 23 to 33 mm. in contrast, none of the strains were inhibited by 100 m ... | 2005 | 16013390 |
| the influence of mayonnaise ph and storage temperature on the growth of listeria monocytogenes in seafood salad. | seafood salad has been identified as a ready-to-eat food with a relatively high incidence of contamination by listeria monocytogenes; however, little is known about the behavior of this pathogen in seafood salad as a function of product ph and storage temperature. to produce data towards the development of a predictive growth model, a 6-strain cocktail of l. monocytogenes was inoculated onto the surface of a shrimp-crabmeat product, mixed with mayonnaise that was previously adjusted with naoh to ... | 2005 | 16014295 |
| occurrence and ribotypes of listeria monocytogenes in gorgonzola cheeses. | in this study 1656 gorgonzola cheeses, collected from october 2003 to april 2004 in the same industrial plant located in lombardia (italy), were analysed in order to evaluate their level of contamination with listeria monocytogenes after packaging, as well as at the end of the shelf life. a subset of gorgonzola isolates was submitted to automated ecori ribotyping to evaluate their dup-ids (dupont identification library code) and their level of genetic diversity. the isolate ribotyping profiles w ... | 2005 | 16014296 |
| gp96 is a receptor for a novel listeria monocytogenes virulence factor, vip, a surface protein. | by comparative genomics, we have identified a gene of the intracellular pathogen listeria monocytogenes that encodes an lpxtg surface protein absent from nonpathogenic listeria species. this gene, vip, is positively regulated by prfa, the transcriptional activator of the major listeria virulence factors. vip is anchored to the listeria cell wall by sortase a and is required for entry into some mammalian cells. using a ligand overlay approach, we identified a cellular receptor for vip, the endopl ... | 2005 | 16015374 |
| genome-wide rnai screen for host factors required for intracellular bacterial infection. | most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. here, we report a genome-wide rna interference screen to identify host factors required for intracellular bacterial pathogenesis. using drosophila cells and the cytosolic pathogen listeria monocytogenes, we identified 305 double-stranded rnas targeting a wide range of cellular functions that altered l. monocytogenes infection. comparison to a similar screen with mycobacterium fortuitum, a vacuola ... | 2005 | 16020693 |
| pyrrolobenzodiazepine dimers: novel sequence-selective, dna-interactive, cross-linking agents with activity against gram-positive bacteria. | pyrrolo[2,1-c][1,4]benzodiazepine (pbd) dimers are synthetic sequence-selective interstrand dna minor-groove cross-linking agents developed from anthramycins. we investigated the antibacterial activity of three dimers, sjg-136, drg-16 and elb-21, which differ in the structure of the pbd monomeric unit and the length of the linker region between the two identical pbd monomers. | 2005 | 16024592 |
| human mitochondria-derived n-formylated peptides are novel agonists equally active on fpr and fprl1, while listeria monocytogenes-derived peptides preferentially activate fpr. | n-formyl peptides are cleavage products of bacterial and mitochondrial proteins, and can attract leukocytes to sites of infection or tissue damage. in this study, hl-60 cell lines expressing the human n-formyl peptide receptor fpr or its two homologues (fprl1, fprl2) were used to determine the receptor selectivity of n-formylated peptides derived from listeria monocytogenes or from human mitochondrial proteins. bacterial peptides were 100-fold more potent on fpr than on fprl1, whereas none of th ... | 2005 | 16025565 |
| longitudinal studies on listeria monocytogenes and other listeria species in two salmon processing plants. | two plants processing salmon fillets and cold smoked salmon were investigated for occurrence of listeria in products and the environment. analyses were conducted for a period of 31 weeks. at plant a, 252 samples were examined of which 97 were from unprocessed fish and 155 from cold-smoked fish. at plant b, 189 samples of unprocessed fish were investigated. the first examination of unprocessed fish at plant a showed a presence of l. monocytogenes and l. spp. in 81% and 19% of the samples respecti ... | 2005 | 16026018 |
| [effect of the constitutive activity of pathogenicity genes in listeria monocytogenes]. | the effect of the constitutive expression of pathogenicity factors in l. monocytogenes was studied on the model of the intraperitoneal infection of mice. the constitutive expression was due to a single amino acid substitution in the transcriptional factor pfra, the master regulator of l. monocytogenes virulence genes. the effective lethal dose (ld50) for the strain with the constitutive expression of pathogenicity factors was 3 times lower than for the isogenic wildtype strain. when introduced i ... | 2005 | 16028503 |
| isolation of listeria monocytogenes from a bite in a cat from the common tree weta (hemideina crassidens). | 1984 | 16031053 | |
| presence and coincidence of listeria spp., motile aeromonads and yersinia enterocolitica in a cold-smoked salmon processing and packing plant. | environmental samples and samples of partially processed fish from a cold-smoked salmon processing and packing plant, and product samples purchased from retail outlets, were examined for the presence of listeria monocytogenes and other listeria spp., motile aeromonads and yersinia enterocolitica. listeria spp. were not isolated from raw fish but were a contaminant of processed and partially processed fish. listeria spp. were also detected in 18.8% of surfaces in contact with fish. unlike listeri ... | 1993 | 16031699 |
| a survey for listeria species, motile aeromonads and yersinia enterocolitica on bovine and ovine carcasses. | one hundred ovine and 100 bovine carcasses in two abattoirs were sampled just after dressing for the presence of yersinia enterocolitica, listeria monocytogenes and motile aeromonads. yersinia enterocolitica was not isolated and only two samples were positive for listeria spp. in both cases, the listeria species were not normally pathogenic to man. in contrast, motile aeromonads were isolated from 81% of ovine and 35% of bovine carcasses. | 1994 | 16031739 |
| microbiological survey of prepackaged pâté and ham in new zealand. | to gauge the effectiveness of pâté and ham manufacturers' management of the microbial safety and quality of their products. | 2005 | 16033505 |
| bacteriological quality of organically grown leaf lettuce in norway. | to investigate bacteriological quality in organically grown leaf lettuce, including the presence of selected pathogenic bacteria, and to obtain information about organic lettuce production, including fertilizing regimes. | 2005 | 16033519 |
| delineation of the function of a major gamma delta t cell subset during infection. | gammadelta t cells play important but poorly defined roles in pathogen-induced immune responses and in preventing chronic inflammation and pathology. a major obstacle to defining their function is establishing the degree of functional redundancy and heterogeneity among gammadelta t cells. using mice deficient in vgamma1+ t cells which are a major component of the gammadelta t cell response to microbial infection, a specific immunoregulatory role for vgamma1+ t cells in macrophage and gammadelta ... | 2005 | 16034115 |
| relative contributions of nk and cd8 t cells to ifn-gamma mediated innate immune protection against listeria monocytogenes. | during the innate immune response to listeria monocytogenes (lm), the secretion of ifn-gamma is crucial in controlling bacterial numbers. we have shown recently that cd8 t cells have the ability to rapidly secrete ifn-gamma independent of ag, in response to il-12 and il-18, during a lm infection. in the current study, we compared the relative abilities of nk and cd8 t cells to provide innate immune protection. upon transfer of either nk or memory ot-i t cells (specific for the ova protein) into ... | 2005 | 16034116 |
| cd43 is required for optimal growth inhibition of mycobacterium tuberculosis in macrophages and in mice. | we explored the role of macrophage (mphi) cd43, a transmembrane glycoprotein, in the pathogenesis of mycobacterium tuberculosis. using gene-deleted mice (cd43-/-), we assessed the association of the bacterium with distinct populations of mphi and found that cd43-/- mphi bound less m. tuberculosis than cd43+/+ mphi. increased infective doses did not abrogate this difference. however, reduced association due to the absence of cd43 could be overcome by serum components. mphi from heterozygote mice, ... | 2005 | 16034122 |
| the tlr7 agonist imiquimod enhances the anti-melanoma effects of a recombinant listeria monocytogenes vaccine. | activation of innate immune cells through tlr triggers immunomodulating events that enhance cell-mediated immunity, raising the possibility that ligands to these receptors might act as adjuvants in conjunction with t cell activating vaccines. in this report, topical imiquimod, a synthetic tlr7 agonist, significantly enhanced the protective antitumor effects of a live, recombinant listeria vaccine against murine melanoma. this tumor protective effect was not dependent on direct application to the ... | 2005 | 16034143 |
| phosphatidylinositol-dependent phospholipases c plc2 and plc3 of candida albicans are dispensable for morphogenesis and host-pathogen interaction. | phospholipases play an important role as virulence factors in human pathogens. candida albicans, the major fungal pathogen of humans, encodes phospholipases of type a, b, c and d. type b plb2 and type d pld1 phospholipases have been shown to contribute to virulence in this organism. we analyzed, in c. albicans, plc2 and plc3, two highly conserved genes coding for phosphatidylinositol-dependent phospholipases c with homology to the known virulence factor plca in the human pathogen listeria monocy ... | 2005 | 16040234 |
| listeriosis prevention knowledge among pregnant women in the usa. | listeriosis is a food-borne disease often associated with ready-to-eat foods. it usually causes mild febrile gastrointestinal illness in immunocompetent persons. in pregnant women, it may cause more severe infection and often crosses the placenta to infect the fetus, resulting in miscarriage, fetal death or neonatal morbidity. simple precautions during pregnancy can prevent listeriosis. however, many women are unaware of these precautions and listeriosis education is often omitted from prenatal ... | 2005 | 16040322 |
| independent protective effects for tumor necrosis factor and lymphotoxin alpha in the host response to listeria monocytogenes infection. | although the essential role of tumor necrosis factor (tnf) in resistance to listeria monocytogenes infection is well established, the roles of the related cytokines lymphotoxin alpha (ltalpha) and lymphotoxin beta (ltbeta) are unknown. using c57bl/6 mice in which the genes for these cytokines were disrupted, we examined the contributions of tnf, ltalpha, and ltbeta in the host response to listeria. to overcome the lack of peripheral lymph nodes in ltalpha(-/-) and ltbeta(-/-) mice, bone marrow c ... | 2005 | 16040991 |
| conditional lethality yields a new vaccine strain of listeria monocytogenes for the induction of cell-mediated immunity. | listeria monocytogenes is a gram-positive intracellular pathogen that can enter phagocytic and nonphagocytic cells and colonize their cytosols. taking advantage of this property to generate an intracellular vaccine delivery vector, we previously described a mutant strain of l. monocytogenes, deltadal deltadat, which is unable to synthesize cell wall by virtue of deletions in two genes (dal and dat) required for d-alanine synthesis. this highly attenuated strain induced long-lived protective syst ... | 2005 | 16041022 |
| 4-1bb (cd137) is required for rapid clearance of listeria monocytogenes infection. | 4-1bb (cd137), a member of the tumor necrosis factor receptor superfamily, is a t-cell-costimulatory receptor that is expressed on activated t cells, dendritic cells, and nk cells. little has been reported about its role in early host defense against bacterial infection. in this study, we report that 4-1bb-deficient (4-1bb(-/-)) mice are much more susceptible to listeria monocytogenes (intracellular bacteria) infections than wild-type mice. upon l. monocytogenes infection, 4-1bb(-/-) mice showed ... | 2005 | 16041031 |
| killed but metabolically active microbes: a new vaccine paradigm for eliciting effector t-cell responses and protective immunity. | we developed a new class of vaccines, based on killed but metabolically active (kbma) bacteria, that simultaneously takes advantage of the potency of live vaccines and the safety of killed vaccines. we removed genes required for nucleotide excision repair (uvrab), rendering microbial-based vaccines exquisitely sensitive to photochemical inactivation with psoralen and long-wavelength ultraviolet light. colony formation of the nucleotide excision repair mutants was blocked by infrequent, randomly ... | 2005 | 16041382 |
| [prevalence of listeria spp. in dairy farm and evaluation of antibiotic-resistance of isolates]. | the study was performed to estimate the prevalence and antimicrobial sensitivities of listeria spp. in raw milk, feaces end environmental samples isolated from 10 dairy in molise region. a total of 454 samples were collected, which comprised 40 raw milk, 40 animal faeces and 374 environmental samples. listeria monocytogenes was never isolated from raw milk specimens; one was isolated from faeces speciments and two were isolated from environmental samples. all isolates were resistant to two or mo ... | 2005 | 16041920 |
| use of hydrogen peroxide in combination with nisin, sodium lactate and citric acid for reducing transfer of bacterial pathogens from whole melon surfaces to fresh-cut pieces. | hydrogen peroxide (2.5%) alone or hydrogen peroxide (1%) in combination with nisin (25 microg/ml), sodium lactate (1%), and citric acid (0.5%) (hplnc) were investigated as potential sanitizers for reducing escherichia coli o157:h7 or listeria monocytogenes populations on whole cantaloupe and honeydew melons. whole cantaloupes inoculated with e. coli o157:h7 and l. monocytogenes at 5.27 and 4.07 log10 cfu/cm2, respectively, and whole honeydew melons inoculated with e. coli o157:h7 and l. monocyto ... | 2005 | 16043249 |
| cpg-activated thy1.2+ dendritic cells protect against lethal listeria monocytogenes infection. | synthetic oligodeoxynucleotides containing cpg motifs (cpg odn) activate the innate immune system by interacting with toll-like receptor 9. the resultant immune response increases host resistance to infection by a variety of pathogenic microorganisms, including listeria monocytogenes. there is a considerable interest in harnessing the immunoprotective properties of cpg odn, yet little is known of the cell phenotype(s) responsible for mediating this protection. this work demonstrates that treatme ... | 2005 | 16047338 |
| rapid purification of recombinant listeriolysin o (llo) from escherichia coli. | listeria monocytogenes is an emerging foodborne pathogen that is responsible for about 28% of the food-related deaths in the united states. it causes meningitis, septicaemia and in pregnant women, abortions and stillbirths. it secretes the toxin listeriolysin o (llo) that allows the bacteria to enter the cytoplasm of host cells, where they can replicate and cause further infection. the rapid and sensitive detection of llo in food samples is a key to monitoring and prevention of listeriosis. to f ... | 2005 | 16052337 |
| antibacterial activity of cerein 8a, a bacteriocin-like peptide produced by bacillus cereus. | the mode of action of cerein 8a, a bacteriocin produced by the soil bacterium bacillus cereus 8a, was investigated. the effect of cerein 8a was tested against listeria monocytogenes and a bactericidal effect at 400 arbitrary units (au)/ml was observed. in addition, cerein 8a was bactericidal against bacillus cereus at 200 au/ml, and inhibited the growth of escherichia coli and salmonella enteritidis. stronger inhibition of these gram-negative bacteria was achieved when the chelating agent edta w ... | 2005 | 16052461 |
| detection of pathogenic bacteria in food samples using highly-dispersed carbon particles. | there is an unmet need for detection methods that can rapidly and sensitively detect food borne pathogens. a flow through immunoassay system utilizing highly dispersed carbon particles and an amperometric technique has been developed and optimized. a sandwich immunoassay format was utilized in which pathogenic cells were captured by antibodies immobilized onto activated carbon particles, and labeled with horseradish peroxidase (hrp) conjugated antibodies. flow of the peroxidase substrates result ... | 2005 | 16076439 |
| evolution and molecular phylogeny of listeria monocytogenes isolated from human and animal listeriosis cases and foods. | to probe the evolution and phylogeny of listeria monocytogenes from defined host species and environments, l. monocytogenes isolates from human (n = 60) and animal (n = 30) listeriosis cases and food samples (n = 30) were randomly selected from a larger collection of isolates (n = 354) obtained in new york state between 1999 and 2001. partial sequencing of four housekeeping genes (gap, prs, purm, and ribc), one stress response gene (sigb), and two virulence genes (acta and inla) revealed between ... | 2005 | 16077098 |
| vaccine wakes from the dead. | 2005 | 16079877 | |
| urocortin 2 suppresses host resistance to listeria monocytogenes infection via up-regulation of interleukin-10. | previous studies have showed that corticotropin-releasing factor (crf) modulates immune response during inflammation. we investigated the effect of crf family peptides on host resistance to listeria monocytogenes infection in mice. when mice were administered ip with crf, urocortin (ucn), or ucn2 30 min prior a sublethal infection with l. monocytogenes, the numbers of bacteria in the organs of ucn2-treated mice were dramatically increased, and most of these mice succumbed. however, host resistan ... | 2005 | 16081642 |
| analysis of pcr-based methods for characterization of listeria monocytogenes strains isolated from different sources. | listeria monocytogenes strains, isolated from various sources (food, environment, and animals), were used to test different pcr-based methods to investigate their capability to define the strain origin. rapd-pcr with three primers and the sau-pcr method, in which the dna was first digested with the sau3a restriction endonuclease and then amplified with a primer designed on the restriction site, were carried out, and the profiles obtained were used to perform cluster analysis. based on the cluste ... | 2005 | 16083819 |
| control of listeria monocytogenes in model sausages by enterocin as-48. | in this work we describe the control of listeria monocytogenes cect 4032 in sausage by adding the enterocin as-48 producer strains enterococcus faecalis a-48-32 and enterococcus faecium s-32-81, and also by adding a semi-purified preparation of the bacteriocin. addition of preformed as-48 caused a significant decrease (p<0.01) in the number of viable listeria even at the lowest bacteriocin concentration tested (112 au/g). at a higher concentration (225 au/g) listeria were below the detection lev ... | 2005 | 16083820 |
| modelling inactivation of listeria monocytogenes by pulsed electric fields in media of different ph. | a study of the effect of square-wave pulsed electric fields (pef) on the inactivation of listeria monocytogenes in mcilvaine buffer of different ph (3.5-7.0) was conducted. l. monocytoges was more pef sensitive at higher electric field strengths (e) and in media of low ph. a treatment at 28 kv/cm for 400 mus that inactivated 1.5, 2.3 and 3.0 log10 cycles at ph 7.0, 6.5 and 5.0 respectively destroyed almost 6.0 log10 cycles at ph 3.5. the general shape of survival curves of l. monocytogenes pef t ... | 2005 | 16083822 |
| inhibition of foodborne bacteria by native and modified protamine: importance of electrostatic interactions. | protamine is a naturally occurring cationic antimicrobial peptide (cap) that has shown some promise for control of microorganisms in food. it was hypothesized that the antibacterial effect is partially due to protamine's electrostatic affinity to the negatively charged cell envelopes of actively growing bacteria. however, nonspecific binding of the caps to negatively charged food particles may reduce the effect in food systems. to test the hypothesis, the antibacterial efficacies of native and r ... | 2005 | 16084263 |
| role for htra in stress induction and virulence potential in listeria monocytogenes. | in silico analysis of the listeria monocytogenes genome revealed lmo0292, a gene predicted to encode a htra-like serine protease. a stable insertion mutant was constructed, revealing a requirement for htra in the listerial response to heat, acid, and penicillin stress. transcriptional analysis revealed that htra is not induced in response to heat shock but is induced in response to low ph and penicillin g stress. furthermore, htra expression was shown to be dependent upon the lisrk two-component ... | 2005 | 16085809 |
| discrimination of listeria monocytogenes contaminated commercial japanese meats. | discrimination was attempted on 14 listeria monocytogenes strains isolated from commercially available japanese pork and chicken. examination of the isolates was performed by restriction fragment length polymorphism (rflp) analysis of the chromosomal dna and amplified products and comparison of the nucleotide sequences of the amplified products. a polymorphism region containing the repeated sequences in the iap gene was amplified by the polymerase chain reaction (pcr). the genetic analyses could ... | 2005 | 16091297 |
| enterocin as-48rj: a variant of enterocin as-48 chromosomally encoded by enterococcus faecium rj16 isolated from food. | the bacteriocinogenic strain rj16 isolated from goat cheese has been identified as enterococcusfaecium by species-specific pcr, dna-rrna hybridization and rdna sequencing. purified bacteriocin from strain rj16 is a carboxypeptidase a-resistant peptide with a molecular mass (7125 da) very close to the cyclic peptide enterocin as-48. bacteriocin from strain rj16 and as-48 show identical antibacterial spectra, although the former is slightly less active on strains of listeria monocytogenes and baci ... | 2005 | 16094865 |
| membrane permeabilization, orientation, and antimicrobial mechanism of subtilosin a. | subtilosin a is an antimicrobial peptide produced by the soil bacterium bacillus subtilis that possesses bactericidal activity against a diverse range of bacteria, including listeria monocytogenes. recent structural studies have found that subtilosin a is posttranslationally modified in a unique way, placing it in a new class of bacteriocins. in this study, in order to understand the mechanism of membrane-disruption by subtilosin a, the interaction of the peptide with model phospholipid bilayers ... | 2005 | 16095584 |
| dna vaccines against enteric infections. | the first dna vaccines for prevention of infectious diseases were described in 1993 and have since been shown to generate protective humoral and cellular immune responses to numerous infectious agents. for enteric infections, protective immunity has been obtained with dna vaccines against several enteric viral, bacterial, and parasitic agents. inoculation of dna vaccines has generally been by intramuscular injection or by gene gun delivery of vaccine dna-coated gold microparticles into the skin. ... | 2006 | 16095769 |
| the cholesterol-dependent cytolysin listeriolysin o aggregates rafts via oligomerization. | the pore-forming toxin listeriolysin o (llo) is the main virulence factor of listeria monocytogenes. llo is known to act as a pseudo cytokine/chemokine, which induces a broad spectrum of host responses that ultimately influences the outcome of listeriosis. in the present study we demonstrate that llo is a potent aggregator of lipid rafts. llo was found to aggregate the raft associated molecules gm1, the gpi-anchored proteins cd14 and cd16 as well as the tyrosine kinase lyn. abrogation of the cyt ... | 2005 | 16098221 |
| listeria monocytogenes ferritin protects against multiple stresses and is required for virulence. | in this study, the role of listeria monocytogenes ferritin was investigated. the fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-shock. we also showed that ferritin provides protection against reactive oxygen species and is essential for full virulence of l. monocytogenes. a comparative proteomic analysis revea ... | 2005 | 16098690 |
| a risk microbiological profile of the australian red meat industry: risk ratings of hazard-product pairings. | a risk profile of microbial hazards across the supply continuum for the beef, sheep and goat meat industries was developed using both a qualitative tool and a semi-quantitative, spreadsheet tool, risk ranger. the latter is useful for highlighting factors contributing to food safety risk and for ranking the risk of various product/pathogen combinations. in the present profile the qualitative tool was used as a preliminary screen for a wide range of hazard-product pairings while risk ranger was us ... | 2005 | 16099063 |
| primary cutaneous listeriosis in a veterinarian. | 2005 | 16100376 | |
| virr, a response regulator critical for listeria monocytogenes virulence. | signature-tagged mutagenesis (stm) was used to identify new genes involved in the virulence of the gram-positive intracellular pathogen listeria monocytogenes. one of the mutants isolated by this technique had the transposon inserted in virr, a gene encoding a putative response regulator of a two-component system. deletion of virr severely decreased virulence in mice as well as invasion in cell-culture experiments. using a transcriptomic approach, we identified 12 genes regulated by virr, includ ... | 2005 | 16102006 |
| the 5' untranslated region-mediated enhancement of intracellular listeriolysin o production is required for listeria monocytogenes pathogenicity. | listeriolysin o (llo) and acta are essential virulence determinants for listeria monocytogenes pathogenesis. transcription of acta and hly, encoding llo, is regulated by prfa and increases dramatically during intracellular infection. the 5' untranslated regions (5' utrs) of acta and prfa have been shown to upregulate expression of their respective gene products. here, we demonstrate that the hly 5' utr plays a critical role in regulating expression of llo during intracellular infection. deletion ... | 2005 | 16102013 |
| gene expression profiling of mouse host response to listeria monocytogenes infection. | the purpose of this study was to evaluate gene expression profiles in the liver and blood for prediction of infection severity from listeria monocytogenes (lm). mice were injected with medium broth (control) or a nonlethal or lethal dose of lm and sacrificed 6 h later. gene expression changes were determined using affymetrix mgu74av2 genechips and confirmed by real-time polymerase chain reaction analysis. we identified discernable genes whose gene expression profiles can be used in pattern recog ... | 2005 | 16102935 |
| solid- and vapor-phase antimicrobial activities of six essential oils: susceptibility of selected foodborne bacterial and fungal strains. | the antimicrobial activity of essential oils (eos) of cinnamon (cinnamon zeylanicum), clove (syzygium aromaticum), basil (ocimum basillicum), rosemary (rosmarinus officinalis), dill (anethum graveolens), and ginger (zingiber officinalis) was evaluated over a range of concentrations in two types of contact tests (solid and vapor diffusion). the eos were tested against an array of four gram-positive bacteria (staphylococcus aureus, bacillus cereus, enterococcus faecalis, and listeria monocytogenes ... | 2005 | 16104824 |
| molecular basis of listeriolysin o ph dependence. | listeriolysin o (llo) is a cholesterol-dependent cytolysin that is an essential virulence factor of listeria monocytogenes. llo pore-forming activity is ph-dependent; it is active at acidic ph (<6), but not at neutral ph. in contrast to other ph-dependent toxins, we have determined that llo pore-forming activity is controlled by a rapid and irreversible denaturation of its structure at neutral ph at temperatures >30 degrees c. rapid denaturation is triggered at neutral ph by the premature unfold ... | 2005 | 16105950 |
| sustained effect of inhaled diesel exhaust particles on t-lymphocyte-mediated immune responses against listeria monocytogenes. | studies have shown that exposure to diesel exhaust particles (dep) suppresses pulmonary host defense against bacterial infection. the present study was carried out to characterize whether dep exposure exerts a sustained effect in which inhaled dep increase the susceptibility of the lung to bacterial infection occurring at a later time. brown norway rats were exposed to filtered air or dep by inhalation at a dose of 21.2 +/- 2.3 mg/m3, 4 h/day for 5 days, and intratracheally instilled with saline ... | 2005 | 16107554 |
| does sporadic listeria gastroenteritis exist? a 2-year population-based survey in nova scotia, canada. | febrile gastroenteritis due to listeria monocytogenes (lm) has been primarily described in foodborne outbreaks. we decided to determine the incidence of sporadic, febrile gastroenteritis due to lm in a large, well-defined north american population over a 2-year period and to compare these cases to sporadic cases of campylobacter and salmonella infections occurring concurrently in the community. | 2005 | 16107973 |
| ability of orally administered ifn-alpha-containing transgenic potato extracts to inhibit listeria monocytogenes infection. | type i interferons (ifn-alpha/beta) were originally thought to be antiviral cytokines, but it has recently been reported that they also play an important role in potentiating innate and adaptive immune responses. moreover, several studies have shown that the oral administration of type i ifn ameliorates various biologic activities. here, we studied the ability of orally administered ifn-alpha to protect mice from systemic listeria monocytogenes infection. daily oral administration of purified na ... | 2005 | 16108729 |
| factors affecting the inactivation of micro-organisms by intense light pulses. | to determine the influence of several factors on the inactivation of micro-organisms by intense light pulses (ilp). | 2005 | 16108787 |
| decrease of enteric micro-organisms from rural sewage sludge during their composting in straw mixture. | to study the decrease of enteric micro-organisms including viable nematode eggs, enteroviruses, faecal indicators (escherichia coli and enterococci) and pathogenic bacteria (listeria monocytogenes, salmonella sp. and clostridium perfringens) of a rural sewage sludge when it is composted for 7 months in mixture with straw. | 2005 | 16108794 |
| screening of benzalkonium chloride resistance in listeria monocytogenes strains isolated during cold smoked fish production. | to determine the susceptibility to disinfectants and cross-resistance to antibiotics in listeria monocytogenes strains isolated from fish products and the fish-processing environment. | 2005 | 16108923 |
| regulation of the bacillus subtilis ytmi operon, involved in sulfur metabolism. | the ytli regulator of bacillus subtilis activates the transcription of the ytmi operon encoding an l-cystine abc transporter, a riboflavin kinase, and proteins of unknown function. the expression of the ytli gene and the ytmi operon was high with methionine and reduced with sulfate. using deletions and site-directed mutagenesis, a cis-acting dna sequence important for ytli-dependent regulation was identified upstream from the -35 box of ytmi. gel mobility shift assays confirmed that ytli specifi ... | 2005 | 16109943 |