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accelerated detection of extended-spectrum β-lactamases in clinical isolates of enterobacteriaceae.a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esblproducers by the standard clinical laboratory standards ...200628700883
insights into a novel blakpc-2-encoding incp-6 plasmid reveal carbapenem-resistance circulation in several enterobacteriaceae species from wastewater and a hospital source in spain.untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. however, its role in the spread of antibiotic resistances in the human population remains poorly investigated. we used whole genome sequencing to analyze 25 kpc-2-producing enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical citrobacter freundii isolates from a tertiary hospital in the same collection ar ...201728702005
introduction of sample tubes with sodium azide as a preservative for ethyl glucuronide in urine.ethyl glucuronide (etg) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. however, the instability of etg in urine against bacterial degradation or the post-collectional synthesis of etg in contaminated samples may cause false interpretation of etg results in urine samples. this study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism ta ...201728712037
imipenem-resistant gram-negative bacterial isolates carried by persons upon medical examination in korea.carbapenem-resistant gram-negative bacteria (cr-gnb) have emerged and disseminated worldwide, become a great concern worldwide including korea. the prevalence of fecal carriage of imipenem-resistant gram-negative bacteria (ir-gnb) in persons in korea was investigated. stool samples were collected from 300 persons upon medical examination. samples were screened for ir-gnb by using macconkey agar with 2 μl/ml imipenem. species were identified by 16s rrna gene sequence analysis, and antimicrobial s ...201728721477
novel multifunctional plant growth-promoting bacteria in co-compost of palm oil industry waste.previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in malaysia. little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. we isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: ...201728736147
novel class 1 integrons and sequence types in vim-2 and vim-11-producing clinical strains of enterobacter cloacae.all vim-producing enterobacteriaceae (six enterobacter cloacae) submitted to the argentinian reference laboratory in antimicrobial resistance in the period 2008-13 were characterized. the isolates were referred from 6 nosocomial institutions located in 5 different cities across the country. all isolates showed carbapenem disk diffusion inhibition zones ≤22mm and synergism between a carbapenem disk and edta/sma. the six isolates were pcr positive for blavim. imipenem mics were ≤1 to 8μg/ml. typin ...201728739428
multidrug resistant bacteria are sensitive to euphorbia prostrata and six others cameroonian medicinal plants extracts.multidrug resistant (mdr) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.201728743314
in-vivo electrochemical monitoring of h2o2 production induced by root-inoculated endophytic bacteria in agave tequilana leaves.a dual-function platinum disc microelectrode sensor was used for in-situ monitoring of h2o2 produced in a. tequilana leaves after inoculation of their endophytic bacteria (enterobacter cloacae). voltammetric experiments were carried out from 0.0 to -1.0v, a potential range where h2o2 is electrochemically reduced. a needle was used to create a small cavity in the upper epidermis of a. tequilana leaves, where the fabricated electrochemical sensor was inserted by using a manual three-dimensional mi ...201728746900
evaluation of the rapid polymyxin np test for polymyxin b resistance detection using enterobacter cloacae and enterobacter aerogenes isolates.background: polymyxin resistance is an increasing problem worldwide. currently, determining susceptibility to polymyxins is problematic and lengthy. polymyxins diffuse poorly into agar, potentially giving inaccurate disk diffusion and etest results. a rapid screening test (2 h) for detection of polymyxin-resistance in enterobacteriaceae, developed by nordmann and poirel (rapid polymyxin np test, 2016), detects glucose metabolization in the presence of polymyxins e and b via ph-induced color chan ...201728747375
antimicrobial effects of lysophosphatidylcholine on methicillin-resistant staphylococcus aureus.methicillin-resistant staphylococcus aureus (mrsa) is an important health care-associated and community-associated pathogen and causes a large number of infections worldwide. for the purpose of application to topical treatment of mrsa infection, we examined the antimicrobial effects of lysophosphatidylcholine (lpc) on mrsa strains. we also investigated the combination effect of lpc and gentamicin on mrsa growth.201728748087
identification of an operon involved in fluoride resistance in enterobacter cloacae frm.fluorine is ubiquitous and the most active non-metal element in nature. while many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. in this study, a fluoride-resistant strain, enterobacter cloacae frm, was identified which could grow well at a fluoride concentration of 4,000 mg/l. according to comparative genomics, tr ...201728754999
draft genome sequence of an aminoglycoside-resistant rmtd-2-producing enterobacter cloacae subsp. cloacae (st)395 in brazil.enterobacter cloacae has recently emerged as an important agent of nosocomial infections, due to the diffusion of extended spectrum β-lactamases and carbapenemases in this species. in this context, a raise in the use of aminoglycosides for therapy was noticed, being followed by the accelerated development of resistance mechanisms. in this study, we report the draft genome sequence of a multidrug-resistant enterobacter cloacae subsp. cloacae strain (ec2), isolated from active surveillance culture ...201728757348
a prospective randomised trial of isolated pathogens of surgical site infections (ssi).every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported.201728761643
[analysis of drug resistance of acinetobacter baumannii in wound of children with traffic injury and its relationship with antibiotic use].objective: to know the drug resistance of acinetobacter baumannii (ab) in wound of children with traffic injury and its relationship with antibiotic use. methods: wound exudate of 226 children with traffic injury admitted to our unit from january 2010 to december 2015 were collected. api bacteria identification panels and fully automatic microbiological identification system were used to identify pathogens. kirby-bauer paper disk diffusion method was used to detect the drug resistance of pathoge ...201728763905
recurrent fuo due to intermittent enterobacter cloacae bacteremias from an infected pacemaker lead diagnosed by gallium scan.fever of unknown origin (fuo) refers to fevers of ≥101° f that persist for ≥3 weeks and remain undiagnosed after a focused inpatient or outpatient workup. fuo may be due to infectious, malignant/neoplastic, rheumatic/inflammatory, or miscellaneous disorders. recurrent fuos are due to the same causes of classical fuos. recurrent fuos may have continuous or intermittent fevers and are particularly difficult to diagnose. with intermittent fever, recurrent fuo diagnostic tests are best obtained duri ...201728768449
photoinactivation of multidrug resistant bacteria by monomeric methylene blue conjugated gold nanoparticles.multidrug resistant (mdr) bacterial infections have become a severe threat to the community health due to a progressive rise in antibiotic resistance. nanoparticle-based photodynamic therapy (pdt) is increasingly been adopted as a potential antimicrobial option, yet the cytotoxicity associated with pdt is quite unspecific. herein, we show concanavalin-a (cona) directed dextran capped gold nanoparticles (gnpdex-cona) enhanced the efficacy and selectivity of methylene blue (mb) induced killing of ...201728778019
antimicrobial resistance of 3 types of gram-negative bacteria isolated from hospital surfaces and the hands of health care workers.there has been an increased focus in recent years on antimicrobial resistance of bacteria isolated from clinical samples. however, resistance of bacteria from hospital environments has been less frequently investigated.201728780198
treatment of gaseous volatile organic compounds using a rotating biological filter.rotating biological filter (rbf), which provides higher oxygen mass transfer has been developed for treating gaseous volatile organic compounds (vocs) such as btex (benzene, toluene, ethylbenzene and xylene) at higher concentrations. the screening of enriched cultures has been done initially to enhance the performance of rbf for treating xylene, toluene and xylene, and btex at various loading rates. the removal efficiency of btex was maximum (82%), higher than toluene and xylene (79%), and xylen ...201728780260
the occurrence of ctx-m-25-producing enterobacteriaceae in day-old broiler chicks in japan.day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between january and july 2016. sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant enterobacteriaceae. cefotaxime (ctx)-resistant klebsiella pneumoniae (1 isolate) and enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. conjugation experiments revealed that th ...201728781343
the first enterobacter cloacae co-producing ndm and oxa-48 carbapenemases and interhospital spread of oxa-48 and ndm-producing klebsiella pneumoniae in turkey.the aim of this study was to investigate the occurrence of carbapenemase-producing enterobacteriaceae.201728792697
high prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in wild fish from the mediterranean sea in algeria.we investigated the prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae among wild fish from the coast of bejaia (algeria) in the mediterranean sea.201728805537
rapid phenotypic stress-based microfluidic antibiotic susceptibility testing of gram-negative clinical isolates.bacteremia is a life-threatening condition for which antibiotics must be prescribed within hours of clinical diagnosis. since the current gold standard for bacteremia diagnosis is based on conventional methods developed in the mid-1800s-growth on agar or in broth-identification and susceptibility profiling for both gram-positive and gram-negative bacterial species requires at least 48-72 h. recent advancements in accelerated phenotypic antibiotic susceptibility testing have centered on the micro ...201728808348
draft genome sequences of two enterobacter cloacae subsp. cloacae strains isolated from australian hematology patients with bacteremia.enterobacter cloacae is a common member of the gut microbiota in healthy individuals. however, it is also an opportunistic pathogen, capable of causing bacteremia. we report the draft genomes of two enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. both isolates carry genes encoding extended-spectrum β-lactamases.201728818892
complete genome sequence of enterobacter cloacae 704sk10, an oxa-48-encoding wastewater isolate.here we present the complete genome sequence of enterobacter cloacae 704sk10, a swiss wastewater isolate encoding an oxa-48 carbapenemase. assembly resulted in closed sequences of the 4,876,946-bp chromosome, a 111,184-bp incf plasmid, and an oxa-48-encoding incl plasmid (63,458 bp) nearly identical to the previously described plasmid poxa-48.201728818912
antimicrobial activity of octenidine against multidrug-resistant gram-negative pathogens.multidrug-resistant (mr) gram-negative (gn) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. well-tolerated antiseptics, such as octenidine dihydrochloride (oct), may be a very useful tool in infection control to reduce the dissemination of mrgn. this study aimed to investigate the bactericidal activity of oct against international epidemic clones of mrgn. a set of five different species ...201728825186
application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products.foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ...201728825446
a simple phenotypic method for screening of mcr-1-mediated colistin resistance.to evaluate a novel method, named colistin-mac test, for phenotypic screening of acquired colistin-resistance mediated by transferable mcr-1 resistance determinants, based on colistin mic reduction in the presence of dipicolinic acid (dpa).201728827120
draft genome sequence of enterobacter cloacae st520 harbouring blakpc-2, blactx-m-15 and blaoxa-17 isolated from coastal waters of the south atlantic ocean.aquatic environments have contributed to the dissemination of multidrug-resistant (mdr) bacteria, representing a risk for humans and animals. the aim of this study was to report the first draft genome sequence of a mdr enterobacter cloacae strain recovered from seawater in a public beach in brazil.201728827199
antimicrobial activity of ceftazidime-avibactam tested against multidrug-resistant enterobacteriaceae and pseudomonas aeruginosa isolates from united states medical centers (2013--2016).the in vitro activity of ceftazidime-avibactam and many comparator agents was determined against various resistant subsets of organisms selected among 36,380 enterobacteriaceae and 7,868 pseudomonas aeruginosa. isolates were consecutively collected from 94 us hospitals, and all isolates were tested for susceptibility by reference broth microdilution methods in a central monitoring laboratory (jmi laboratories). enterobacteriaceae isolates resistant to carbapenems (cre) and/or ceftazidime-avibact ...201728827415
transmission and characterization of bla ndm-1 in enterobacter cloacae at a teaching hospital in yunnan, china.in recent years, new delhi metallo-beta-lactamases 1 (bla ndm-1) has been reported with increasing frequency and become prevalent. the present study was undertaken to investigate the epidemiological dissemination of the bla ndm-1 gene in enterobacter cloacae isolates at a teaching hospital in yunnan, china.201728830556
persistent gram-negative neurosurgical meningitis in a neonate, successfully treated with intraventricular colistin: case report and review of the literature.we present a case of gram-negative neurosurgical meningitis in a neonate, refractory to optimal intravenous therapy and removal of ventriculo-peritoneal shunt. cerebrospinal fluid was sterilized within 24 hours using intraventricular colistin. this is the first report of intraventricular colistin use in neonatal meningitis caused by enterobacter cloacae.201728841583
high mortality rate associated with kpc-producing enterobacter cloacae in a brazilian hospital.we describe a clonal dissemination of kpc-producing enterobacter cloacae in a brazilian hospital. patients diagnosed with theses isolates showed high mortality rate (41.8%) and were associated with previous use of antibiotics and urinary catheterization. therefore, infection control measures and use of stricter antibiotic policies are required to control the spread of these organisms.201728844375
isolation and identification of nonoral pathogenic bacteria in the oral cavity of patients with removable dentures.dentures in the oral cavity may act as a reservoir of microorganisms, which may be related to systemic infections. the aim of this study was to investigate the nonoral pathogenic bacteria in the oral cavity of patients with removable dentures in shiraz, southern iran.201728852636
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