Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [the contribution of system regulation of pathogenicity-determining genes expression to the virulence of facultative intracellular parasites]. | bacteria which belong to the group of facultative parasites are capable of intensive proliferation both outside the organism and inside eukaryotic cells, thus often causing severe human and animal illness. the necessity of quick adaptation to the alteration of conditions after intrusion into the macro-organism gave rise to the development of complex genetic systems, regulating the activity of various gene groups. the review, written by the example of two pathogen bacteria, the gram-positive list ... | 2005 | 15715156 |
| contamination of chicken carcasses in gauteng, south africa, by salmonella, listeria monocytogenes and campylobacter. | the presence of the foodborne pathogens, salmonella spp., listeria monocytogenes and campylobacter spp., on 99 fresh and frozen chicken carcasses sourced from various retailers in gauteng, south africa, was investigated. using culture methods, 60.6% of the carcasses were found to be contaminated with one or more pathogens, with 19.2%, 19.2% and 32.3% of the carcasses being found to harbour salmonella, l. monocytogenes and campylobacter, respectively. the extent of contamination with one or more ... | 2005 | 15718024 |
| interactions of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes plants cultivated in a gnotobiotic system. | the growth and persistence of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes on a diverse range of plant types over extended cultivation periods was studied. when introduced on the seed of carrot, cress, lettuce, radish, spinach and tomato all the pathogens became rapidly established shortly after germination, attaining cell densities of the order of 5.5-6.5 log cfu/g. in general, es. coli o157:h7 and l. monocytogenes became established and persisted at significantly ... | 2005 | 15718025 |
| survival of pathogenic bacteria in pesticide solutions and on treated tomato plants. | the ability of salmonella, escherichia coli o157:h7, listeria monocytogenes, and shigella to survive or grow in pesticide solutions (ambush 240ec, benlate t-n-g, bravo 500, botran 75wp, captan 80wdg, parasol, and vendex 50w) used by the horticultural industry was examined. in the laboratory, individual cultures were inoculated at 4 log cfu/ml in pesticides diluted with sterile saline to the lowest recommended spray concentrations. during 21 degrees c incubation for < or =96 h, bacterial survivor ... | 2005 | 15726972 |
| determination of 5-log pathogen reduction times for heat-processed, acidified vegetable brines. | recent outbreaks of acid-resistant food pathogens in acid foods, including apple cider and orange juice, have raised concerns about the safety of acidified vegetable products. we determined pasteurization times and temperatures needed to assure a 5-log reduction in the numbers of escherichia coli o157:h7, listeria monocytogenes, and salmonella strains in acidified cucumber pickle brines. cocktails of five strains of each pathogen were (separately) used for heat-inactivation studies between 50 an ... | 2005 | 15726973 |
| alternative sigma factor sigmab is not essential for listeria monocytogenes surface attachment. | listeria monocytogenes is a foodborne pathogen frequently isolated from the food processing environment. multiple lines of evidence suggested a possible role for the l. monocytogenes alternative transcription factor sigma b (sigmab) in surface attachment and biofilm formation. therefore, through plate count and microscopic techniques, the l. monocytogenes 10403s strain and an otherwise isogenic deltasigb strain were tested for attachment to stainless steel. analysis of microscopic images reveale ... | 2005 | 15726974 |
| effectiveness of irradiation treatments in inactivating listeria monocytogenes on fresh vegetables at refrigeration temperature. | ionizing radiation can be effective in controlling the growth of food spoilage and foodborne pathogenic bacteria. this study reports on an investigation of the effectiveness of irradiation treatment to eliminate listeria monocytogenes on laboratory-inoculated broccoli, cabbage, tomatoes, and mung bean sprouts. irradiation of broccoli and mung bean sprouts at 1.0 kgy resulted in reductions of approximately 4.88 and 4.57 log cfu/g, respectively, of a five-strain cocktail of l. monocytogenes. reduc ... | 2005 | 15726975 |
| survival of listeria monocytogenes in experimental chorizos. | chorizos-mexican-style raw-meat sausages-are a concern in california because their production in small ethnic food markets is unregulated. their formulation may cause them to appear cooked to the consumer, who may eat the raw sausage without prior proper cooking. bacterial pathogens in such products may cause illness or even death. survivability of listeria monocytogenes in chorizos was evaluated under different storage conditions selected on the basis of an initial survey of uninspected chorizo ... | 2005 | 15726976 |
| simultaneous detection of listeria monocytogenes in chicken meat enrichments by pcr and reverse-transcription pcr without dna/rna isolation. | environmental and food samples can be analyzed using pcr and reverse transcription (rt)-pcr techniques to discriminate between viable and nonviable cells of bacterial pathogens. here, we describe the use of a commercial lysis buffer, initially designed for mammalian cells, that permits the rapid extraction of bacterial dna and rna. the buffer is an rt-pcr-compatible lysis solution in which rna is stable and can be frozen for later use. rt-pcr is carried out directly after dnase i treatment of cr ... | 2005 | 15726988 |
| incidence of listeria monocytogenes in raw seafood products in japanese retail stores. | the incidence of listeria monocytogenes in raw fish, shellfish, and fish roe was investigated in seafood products collected from randomly selected retail stores in and around tokyo, japan. of the 10 samples of 208 examined found positive for l. monocytogenes by mini-vidas lmo, seven were fish roe (cod, salmon) and three were minced tuna. three serotypes (1/2a, 1/2b, 3b) were detected among the isolated strains; serotype 1/2a was predominant (8 of 10). | 2005 | 15726989 |
| bacteriophages as biocontrol agents in food. | bacteriophages possess attributes that appear to be attractive to those searching for novel ways to control foodborne pathogens and spoilage organisms. these phages have a history of safe use, can be highly host specific, and replicate in the presence of a host. campylobacter, salmonella, and listeria monocytogenes and various spoilage organisms have responded to phage control on some foods. however, the use of phages as biocontrol agents is complicated by factors such as an apparent requirement ... | 2005 | 15726992 |
| cutting edge: microbial products elicit formation of dendritic cell aggresome-like induced structures in macrophages. | in response to a maturation stimulus, dendritic cells undergo the formation of ubiquitinated protein aggregates known as dendritic cell aggresome-like induced structures (dalis). dalis are thought to act as ag storage structures, allowing for the prioritized degradation of proteins during infection. in this study, we demonstrate that murine macrophages can also form ubiquitinated protein aggregates that are indistinguishable from dalis. these were formed in a dose- and time-dependent manner, and ... | 2005 | 15728449 |
| molecular analysis of resistance to streptogramin a compounds conferred by the vga proteins of staphylococci. | the vga and msr resistance determinants, encoded by mobile genetic elements in various staphylococcal strains, belong to a family of atp-binding cassette (abc) proteins whose functions and structures are ill defined. their amino acid sequences are similar to those of proteins involved in the immunity of streptomycetes to the macrolide-lincosamide-streptogramin antibiotics that they produce. sequence analysis of the genomes of the gram-positive bacteria with low g+c contents revealed that lmo0919 ... | 2005 | 15728891 |
| listeria monocytogenes exploits erm protein functions to efficiently spread from cell to cell. | cell-to-cell spread is a fundamental step in the infection cycle of listeria monocytogenes that strictly depends on the formation of bacteria-induced protrusions. since listeria actin tails in the protrusions are tightly associated with the plasma membrane, we hypothesised that membrane-cytoskeleton linkers would be required for initiating and sustaining their formation and the subsequent cell-to-cell spread. we have found that ezrin, a member of the ezrin, radixin and moesin (erm) family that f ... | 2005 | 15729356 |
| comparative activity of quinolones (ciprofloxacin, levofloxacin, moxifloxacin and garenoxacin) against extracellular and intracellular infection by listeria monocytogenes and staphylococcus aureus in j774 macrophages. | quinolones accumulate in eukaryotic cells and show activity against a large array of intracellular organisms, but systematic studies aimed at examining their pharmacodynamic profile against intracellular bacteria are scarce. the present work aims at comparing intracellular-to-extracellular activities in this context. | 2005 | 15731197 |
| acid-adaptation does not increase the resistance of listeria monocytogenes to irradiation in a seafood salad. | stress adaptation of microbial cells enables the cells to survive better when they are subsequently exposed to other types of stresses. in the food industry, pathogens are commonly stressed during food processing and this is a concern where pathogens such as listeria monocytogenes are involved. research was conducted to determine if acid adaptation of l. monocytogenes provides resistance to ionizing irradiation. three different strains of l. monocytogenes were acid-adapted using three different ... | 2005 | 15734563 |
| treatment of brain abscess caused by listeria monocytogenes in a patient with allergy to penicillin and trimethoprim-sulfamethoxazole. | 2005 | 15736033 | |
| production of bacteriocins by coagulase-negative staphylococci involved in bovine mastitis. | in the present study, 188 coagulase-negative staphylococcus (cns) strains were isolated from bovine mastitis cases from 56 different brazilian dairy herds, located in the southeast region of the country, and were tested for antimicrobial substance production. twelve cns strains (6.4%) exhibited antagonistic activity against a corynebacterium fimi indicator strain. most antimicrobial substances were sensitive to proteolytic enzymes suggesting that they might be bacteriocins (bac). amongst the cns ... | 2005 | 15737474 |
| scant activation of cd8 t cells by antigen loaded on heat shock protein. | heat shock proteins (hsp) not only function as chaperones for denatured proteins but also for antigenic peptides, thus inducing protective t cell responses. here we show that vaccination with peptide-loaded hsp70 causes initial interferon-gamma production by murine cd8 t cells but no t cell expansion. these cd8 t cells lacked cytotoxic activity in vitro and in vivo, which was not due to apoptosis. restimulation with peptide-pulsed dendritic cells both bypassed the proliferative block and suspend ... | 2005 | 15739167 |
| the synthetic form of a novel chicken beta-defensin identified in silico is predominantly active against intestinal pathogens. | antimicrobial peptides are essential components of innate immunity and are generally thought to act by disrupting the membrane integrity of microbes. here we report the discovery of two novel chicken beta-defensins, gallinacin (gal)-11 and gal-12, found by hidden markov model profile searching of the chicken genome. we have sequenced the genes and elucidated the 3'utr of gal-11. differential mrna expression of these novel genes has been shown across a panel of chicken tissues. gal-11 mrna was hi ... | 2005 | 15744537 |
| enhanced survival of salmonella enterica in vesicles released by a soilborne tetrahymena species. | nondestructive ingestion by soilborne protozoa may enhance the environmental resiliency of important bacterial pathogens and may model how such bacteria evade destruction in human macrophages. here, the interaction of salmonella enterica serovar thompson with a soilborne tetrahymena sp. isolate was examined using serovar thompson cells labeled with the green fluorescent protein. the bacteria were mixed in solution with cells of tetrahymena at several ratios. during incubation with serovar thomps ... | 2005 | 15746361 |
| a novel role of il-15 in early activation of memory cd8+ ctl after reinfection. | a rapid induction of effector functions in memory t cells provides rapid and intensified protection against reinfection. to determine potential roles of il-15 in early expansion and activation of memory cd8+ t cells in secondary immune response, we examined the cell division and cytotoxicity of memory cd8+ t cells expressing ova(257-264)/kb-specific tcr that were transferred into il-15-transgenic (tg) mice, il-15 knockout (ko) mice, or control c57bl/6 mice followed by challenge with recombinant ... | 2005 | 15749896 |
| establishment of a universal size standard strain for use with the pulsenet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. | the pulsenet national database, established by the centers for disease control and prevention in 1996, consists of pulsed-field gel electrophoresis (pfge) patterns obtained from isolates of food-borne pathogens (currently escherichia coli o157:h7, salmonella, shigella, and listeria) and textual information about the isolates. electronic images and accompanying text are submitted from over 60 u.s. public health and food regulatory agency laboratories. the pfge patterns are generated according to ... | 2005 | 15750058 |
| tumor sensitivity to ifn-gamma is required for successful antigen-specific immunotherapy of a transplantable mouse tumor model for hpv-transformed tumors. | many human tumors lose responsiveness to ifn-gamma, providing a possible mechanism for the tumor to avoid immune recognition and destruction. here we investigate the importance of tumor responsiveness to ifn-gamma in the successful immunotherapy of tc1 tumors that were immortalized with human papillomavirus proteins e6 and e7. | 2004 | 15750832 |
| septic arthritis due to listeria monocytogenes in a patient receiving etanercept. | 2005 | 15751076 | |
| acid-tolerant listeria monocytogenes persist in a model food system fermented with nisin-producing bacteria. | to investigate the induction of the acid tolerance response (atr) in listeria monocytogenes and to assess the persistence of the pathogen in broth fermented using a nisin-producing starter culture. | 2005 | 15752211 |
| detection of viable and dead listeria monocytogenes on gouda-like cheeses by real-time pcr. | surface contamination by listeria monocytogenes of gouda-like cheeses during processing represents a potential public health problem. the aim of this work was to develop novel real-time pcr diagnostics to detect the presence of viable, dead or viable but not culturable (vbnc) cells on gouda-like cheeses. | 2005 | 15752222 |
| the dps-like protein fri of listeria monocytogenes promotes stress tolerance and intracellular multiplication in macrophage-like cells. | members of the ferritin-like dps protein family are found in a number of bacterial species, where they demonstrate the potential to bind iron, and have been implicated in tolerance to oxidative stress. in this study of the food-borne pathogen listeria monocytogenes, the fri gene encoding a dps homologue was deleted, and, compared to wild-type cells, it was found that the resulting mutant was less resistant to hydrogen peroxide, and demonstrated reduced survival following long-term (7-11 days) in ... | 2005 | 15758237 |
| a defective th1 response of the spleen in the initial phase may explain why splenectomy helps prevent a listeria infection. | listeria monocytogenes (listeria) are known to grow and proliferate in the liver while a splenectomy induces host resistance against a listeria infection despite the fact that a splenectomy inhibits the th1 response. therefore, the mechanism by which a splenectomy helps to prevent the growth of listeria still remains to be elucidated. after an i.v. challenge of listeria (1 x 10(6) cfu) in c57bl/6 mice, listeria rapidly increased in the spleen but not in the liver until 48 h. however, after this ... | 2005 | 15762870 |
| evidence for involvement of peptidoglycan in the triggering of an oxidative burst by listeria monocytogenes in phagocytes. | we have shown previously that in listeric encephalitis of cattle and rats, nitrotyrosine was produced in microabscesses, implying that both superoxide anion (o(2) (-)) and nitric oxide (no) are present and react with each other. evidence of local synthesis of no by macrophages was provided, but the source of o(2) (-) remained unknown. here we have examined whether phagocytes exposed to viable and heat-killed listeria monocytogenes (lmdelta) produce o(2) (-) and, if so, whether this results from ... | 2005 | 15762877 |
| [brain abscesses due to listeria monocytogenes]. | listeria monocytogenes is a gram-positive bacillus which causes sporadic infections in immunocompromised humans, with a special propensity for the central nervous system, in the form of acute, subacute or chronic meningitis, rhombencephalitis or abscesses in the brain or spinal cord. the final diagnosis is established by germ culture in blood or in cerebrospinal fluid (csf). preferred treatment is ampicillin in association with aminoglycosides. | 2005 | 15765316 |
| [sepsis caused by listeria monocytogenes related with the use of infliximab]. | 2005 | 15766516 | |
| listeria monocytogenes gastroenteritis in sheep. | 2004 | 15768083 | |
| wasp-related proteins, abi1 and ena/vasp are required for listeria invasion induced by the met receptor. | internalisation of the pathogenic bacterium listeria monocytogenes involves interactions between the invasion protein inlb and the hepatocyte growth factor receptor, met. using colocalisation studies, dominant-negative constructs and small interfering rna (sirna), we demonstrate a cell-type-dependent requirement for various wasp-related proteins in listeria entry and inlb-induced membrane ruffling. the wave2 isoform is essential for inlb-induced cytoskeletal rearrangements in vero cells. in hela ... | 2005 | 15769844 |
| one-year (2003) nationwide pork carcass microbiological baseline data survey in taiwan. | from january through december 2003, swab samples from 1,650 pork carcasses were collected from 39 slaughter plants in taiwan. these samples were analyzed for the prevalence of indicator microorganisms and specific pathogens. viable aerobic bacteria, total coliforms, and escherichia coli were recovered from 100, 95.3, and 87.5% of these carcasses, respectively. of those carcasses that harbored bacteria, the mean aerobic plate, total coliform, and escherichia coli counts were 4.0, 0.6, and 0.1 log ... | 2005 | 15771166 |
| elimination of listeria monocytogenes biofilms by ozone, chlorine, and hydrogen peroxide. | this study evaluated the efficacy of ozone, chlorine, and hydrogen peroxide to destroy listeria monocytogenes planktonic cells and biofilms of two test strains, scott a and 10403s. l. monocytogenes was sensitive to ozone (o3), chlorine, and hydrogen peroxide (h2o2). planktonic cells of strain scott a were completely destroyed by exposure to 0.25 ppm o3 (8.29-log reduction, cfu per milliliter). ozone's destruction of scott a increased when the concentration was increased, with complete eliminatio ... | 2005 | 15771172 |
| inhibitory effects of organic acid salts for control of listeria monocytogenes on frankfurters. | sodium diacetate (sd), sodium diacetate plus potassium benzoate (sd-pb), and sodium lactate plus sodium diacetate plus potassium benzoate (sl-sd-pb) were selected for initial effectiveness against listeria monocytogenes on frankfurters. treatments were evaluated at -2.2, 1.1, 4.4, 10.0, and 12.8 degrees c for up to 90 days. the compounds were applied as 3 or 6% (total concentration) dipping solutions for surface treatment of the frankfurters. the treated frankfurters were inoculated with a five- ... | 2005 | 15771173 |
| eradicating listeria monocytogenes from fully cooked franks by using an integrated pasteurization-packaging system. | surface pasteurization by applying steam or hot water before or after packaging of processed foods may be used to eliminate pathogens such as listeria monocytogenes from ready-to-eat meat and poultry products. surface pasteurization treatment with a mixture of pressurized steam and hot water was integrated into a continuous vacuum-packaging system to reduce l. monocytogenes from fully cooked franks. the franks (2.54 cm diameter by 15.24 cm length) were surface inoculated to contain up to 6 log c ... | 2005 | 15771174 |
| destruction of listeria monocytogenes in sturgeon (acipenser transmontanus) caviar by a combination of nisin with chemical antimicrobials or moderate heat. | the objective of this study was to investigate the effect of nisin in combination with heat or antimicrobial chemical treatments (such as lactic acid, chlorous acid, and sodium hypochlorite) on the inhibition of listeria monocytogenes and total mesophiles in sturgeon (acipenser transmontanus) caviar. the effects of nisin (250, 500, 750, and 1,000 iu/ml), lactic acid (1, 2, and 3%), chlorous acid (134 and 268 ppm), sodium hypochlorite (150 and 300 ppm), and heat at 60 degrees c for 3 min were eva ... | 2005 | 15771175 |
| multiplex pcr for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 in meat samples. | a multiplex pcr method was developed for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 in meat samples. dna detection sensitivity for this method was 10(3) cfu/ml for each pathogen. when this protocol was used for the detection of each of the above pathogenic bacteria in spiked pork samples, 1 cell per 25 g of inoculated sample could be detected within 30 h. in the samples of naturally contaminated meat, salmonella spp., l. monocytogenes, and e. ... | 2005 | 15771181 |
| effects of suspension in emulsified wiener or incubation in wiener packages on the virulence of listeria monocytogenes scott a in intragastrically inoculated a/j mice. | several outbreaks of listeriosis have been associated with contamination of wieners and other ready-to-eat meat products. in this study, we addressed the question of whether emulsification in, or growth on, wieners triggers a response in the listerial cells that makes them more virulent or protects them against the harsh environment of the gastrointestinal tract in mice. our results indicate that listeria monocytogenes scott a grows poorly, if at all, in one brand of commercially prepared wiener ... | 2005 | 15771188 |
| enhanced plasmid dna delivery using anionic lpdii by listeriolysin o incorporation. | a major obstacle to achieving effective dna-based therapeutics is efficient delivery of the dna to its site of action in the cell. upon internalization by endocytosis, the endosomal membrane represents a critical physical barrier preventing access of dna to the cell cytosol. in order to overcome the membrane barrier and facilitate cytosolic entry, the endosomolytic bacterial protein listeriolysin o (llo) is a potentially promising agent. | 2005 | 15776501 |
| [listeria disease. the first 2 cases reported at the principal hospital of dakar]. | infection disease due to listeria monocytogenes, which is a ubiquitous positive gram bacillus to the essentially alimentary transmission, listeriosis happens on patients presenting an immunodeficiency. the authors report the two first cases of listeriosis diagnosed at hopital principal de dakar. the first case was observed on a 73 years old man, hospitalised for a feverish coma scored at 9 using glasgow scale, with neither meningitis syndrome, nor sign of neurological localisation. the analysis ... | 2003 | 15776652 |
| yersinia outer protein p inhibits cd8 t cell priming in the mouse infection model. | pathogenic yersiniae translocate a mixture of effector proteins called yersinia outer proteins (yops) into the cytosol of eukaryotic cells by their type iii secretion system. yopp is one of the best characterized of these effector proteins and known to inhibit the proinflammatory response of the host by interfering with nf-kappab signal transduction and inducing apoptosis of macrophages. the effects of yopp on the immune response were studied by a yersinia ag-independent approach using bacteria ... | 2005 | 15778387 |
| protective cd8 t cell immunity triggered by cpg-protein conjugates competes with the efficacy of live vaccines. | in contrast to infectious (live) vaccines are those based on subunit ag that are notoriously poor in eliciting protective cd8 t cell responses, presumably because subunit ags become insufficiently cross-presented by dendritic cells (dcs) and because the latter need to be activated to acquire competence for cross-priming. in this study, we show that cpg-ag complexes overcome these limitations. ova covalently linked to cpg-dna (cpg-ova complex), once it is efficiently internalized by dcs via dna r ... | 2005 | 15778402 |
| isolation and pcr amplification of a species-specific oxidoreductase-coding gene region in listeria grayi. | listeria grayi is a nonpathogenic gram-positive bacterium that demonstrates considerable similarities to other members in the genus listeria, including the foodborne human pathogen listeria monocytogenes and the animal pathogen listeria ivanovii. a rapid diagnostic test to identify and diagnose listeriosis would be valuable, especially in cases where the presence of l. grayi may complicate diagnosis. this test would be based on a unique gene present in l. grayi. in this study, after comparative ... | 2005 | 15782240 |
| pleiotropic enhancement of bacterial pathogenesis resulting from the constitutive activation of the listeria monocytogenes regulatory factor prfa. | listeria monocytogenes is a facultative intracellular bacterial pathogen that causes serious disease in immunocompromised individuals, pregnant women, and neonates. bacterial virulence is mediated by the expression of specific gene products that facilitate entry into host cells and enable bacterial replication; the majority of these gene products are regulated by a transcriptional activator known as prfa. l. monocytogenes strains containing prfa e77k or prfa g155s mutations exhibit increased exp ... | 2005 | 15784531 |
| redundant roles for met docking site tyrosines and the gab1 pleckstrin homology domain in inlb-mediated entry of listeria monocytogenes. | the bacterial pathogen listeria monocytogenes causes food-borne illnesses leading to gastroenteritis, meningitis, or abortion. listeria induces its internalization into some mammalian cells through interaction of the bacterial surface protein inlb with host met receptor tyrosine kinase. binding of inlb leads to phosphorylation of met and the adapter gab1 and to activation of host phosphoinositide (pi) 3-kinase. the mammalian ligand of met, hepatocyte growth factor, promotes cell motility and mor ... | 2005 | 15784547 |
| mouse paneth cell secretory responses to cell surface glycolipids of virulent and attenuated pathogenic bacteria. | mouse paneth cells respond to bacteria and bacterial cell surface antigens by discharging secretory granules into the lumen of small intestinal crypts (t. ayabe et al., nat. immunol. 1:113-118, 2000). to investigate mechanisms regulating these responses, purified surface glycolipid molecules with known acyl chain modifications and attenuated properties were tested for the ability to stimulate paneth cell secretion. the antigens included lipopolysaccharide (lps) from wild-type and msbb-null esche ... | 2005 | 15784576 |
| development of a sandwich elisa for the detection of listeria spp. using specific flagella antibodies. | five monoclonal antibodies (mabs) and chicken immunoglobulin (igy) were developed by immunizing with flagella purified from listeria monocytogenes 4b and the five mabs have been confirmed to be specific against three different epitopes of flagellin. the antibodies showed specific reaction to listeria genus and no cross-reactivity with other bacteria tested in this experiment including e.coli o157:h7 and salmonella enteritidis. sandwich enzyme-linked immunosorbent assays (elisa) using the mabs an ... | 2005 | 15785122 |
| [listeria monocytogenes detection in different food products and environmental samples from a large chain of supermarkets in the city of bahía blanca (argentina)]. | this work on listeria monocytogenes detection in different foods was carried out between january 2002 and july 2003. ninety cold-served cooked meats, sliced and packaged by different methods and 132 pieces of soft cheeses were studied. these products were analyzed using the presence/absence in 25 g criterion. l. monocytogenes was not found either in foods sliced over the counter or in controlled cheeses, but it was found in 10% of sliced cold-served foods and 5% of cut and cold-served meats vacu ... | 2004 | 15786871 |
| molecular aspects of listeria monocytogenes infection. | listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection. this review is focused on the role of secreted proteins, including listeriolysin o and two distinct phospholipases c, in modulating the signal transduction of infected cells. | 2004 | 15787192 |
| analysis of the peptidoglycan hydrolases of listeria monocytogenes: multiple enzymes with multiple functions. | listeria monocytogenes is a ubiquitous gram-positive, rod-shaped, widespread in nature, facultative intracellular human and animal pathogen that causes infections collectively termed listeriosis. l. monocytogenes egd encodes a total of 133 surface proteins, the abundance of which, as well as the variety of anchoring systems, probably reflects the ability of this bacterium to survive in diverse environments and to interact with many kinds of eukaryotic cells. the group of surface proteins also in ... | 2004 | 15787194 |
| polymerizer-mediated intracellular movement. | bacterial movement inside the cytoplasm is a major virulence factor in that it is necessary for efficient colonization of the infected tissues. molecules from both the host and the pathogen present possible sites of pharmacologic intervention. because locomoting listeria and shigella mimic the activated state of the leading edge of nonmuscle cells, these pathogens are powerful tools for dissecting the molecular machinery of actin-based motility. analysis of the movement linked to cytoskeleton ma ... | 2004 | 15787195 |
| the antimicrobial activity of extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent. | the antimicrobial activity and the mic values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. at least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus vulgaris, salmonella ... | 2005 | 15787241 |
| uncertainty distribution associated with estimating a proportion in microbial risk assessment. | the uncertainty associated with estimates should be taken into account in quantitative risk assessment. each input's uncertainty can be characterized through a probabilistic distribution for use under monte carlo simulations. in this study, the sampling uncertainty associated with estimating a low proportion on the basis of a small sample size was considered. a common application in microbial risk assessment is the estimation of a prevalence, proportion of contaminated food products, on the basi ... | 2005 | 15787755 |
| meta-analysis of food safety information based on a combination of a relational database and a predictive modeling tool. | the management of microbial risk in food products requires the ability to predict growth kinetics of pathogenic microorganisms in the event of contamination and growth initiation. useful data for assessing these issues may be found in the literature or from experimental results. however, the large number and variety of data make further development difficult. statistical techniques, such as meta-analysis, are then useful to realize synthesis of a set of distinct but similar experiences. moreover ... | 2005 | 15787758 |
| elimination of escherichia coli o 157 : h7 and listeria monocytogenes from raw beef sausage by gamma-irradiation. | the effectiveness of low gamma-irradiation doses in the destruction of escherichia coli o 157 : h7 and listeria monocytogenes in raw beef sausages was investigated. raw samples of fresh manufactured beef sausage were subjected to gamma-irradiation at doses of 0, 1, 2, and 3 kgy. then samples were cold-stored (4 +/- 1 degrees c) for 12 days and the effects of irradiation and storage on the counts of these harmful bacteria were studied. moreover, the effects of irradiation and storage on the perce ... | 2005 | 15789371 |
| listeria monocytogenes meningitis in a patient receiving etanercept for still's disease. | 2005 | 15789900 | |
| murein-hydrolyzing activity of flagellin flaa of listeria monocytogenes. | in this preliminary report we show that a 29 kda surface protein of listeria monocytogenes egd removed from cells with 4 m licl has peptidoglycan (murein) hydrolyzing activity, as revealed by zymographic analysis using bacillus subtilis murein and heat-killed micrococcus luteus cells casted in the gel. following two-dimensional electrophoresis, the protein was electroblotted to pvdf membrane and its identity (flaa) was revealed by sequencing. peptidoglycan hydrolysing activity of flaa purified b ... | 2004 | 15790072 |
| antimicrobial susceptibilities of listeria monocytogenes strains isolated from 2000 to 2002 in poland. | minimal inhibitory concentration (mic) of ampicillin, penicillin, gentamicin, erythromycin, clarithromycin, sulfisoxazole and trimethoprim for 73 listeria monocytogenes isolates from clinical material samples, food and environment was determined using broth microdilution method. with exception of the sulfonamide all antibacterial agents were active against all tested strains. resistance to sulfisoxazole (mic > or = 512 microg/ml) was detected in case of 30.1% of isolates including 5 of 14 human ... | 2004 | 15790078 |
| [meningitis caused by listeria monocytogenes in a patient with myasthenia gravis being treated with tacrolimus]. | 2005 | 15795876 | |
| differential cytosolic delivery and presentation of antigen by listeriolysin o-liposomes to macrophages and dendritic cells. | delivery of antigenic protein to the cytosol of antigen-presenting cells (apcs), such as macrophages (mphi) and dendritic cells (dcs), is required for an efficient cd8 t-cell-mediated immune response. we have previously shown that co-encapsulation of antigenic protein inside ph-sensitive liposomes with listeriolysin o (llo), a pore-forming protein of listeria monocytogenes, generates efficient major histocompatibility complex class i (mhc i)-restricted immune responses both in vitro and in vivo. ... | 2005 | 15804180 |
| super paramagnetic clustering of protein sequences. | detection of sequence homologues represents a challenging task that is important for the discovery of protein families and the reliable application of automatic annotation methods. the presence of domains in protein families of diverse function, inhomogeneity and different sizes of protein families create considerable difficulties for the application of published clustering methods. | 2005 | 15804359 |
| the metalloprotease of listeria monocytogenes controls cell wall translocation of the broad-range phospholipase c. | listeria monocytogenes is a gram-positive bacterial pathogen that multiplies in the cytosol of host cells and spreads directly from cell to cell. during cell-to-cell spread, bacteria become temporarily confined to secondary vacuoles. the broad-range phospholipase c (pc-plc) of l. monocytogenes contributes to bacterial escape from secondary vacuoles. pc-plc requires cleavage of an n-terminal propeptide for activation, and mpl, a metalloprotease of listeria, is involved in the proteolytic activati ... | 2005 | 15805506 |
| enhanced synthesis of internalin a in aro mutants of listeria monocytogenes indicates posttranscriptional control of the inlab mrna. | listeria monocytogenes mutants with deletions in aroa, arob, or aroe exhibited strong posttranscriptional upregulation of internalin a (inla) and inlb synthesis, which resulted in a more-than-10-fold increase in inla-mediated internalization by epithelial caco-2 cells and a 4-fold increase in inlb-mediated internalization by microvascular endothelial cells (human brain microvascular endothelial cells) compared to the wild-type strain. the increase in inla and inlb production was not due to enhan ... | 2005 | 15805530 |
| [evaluation of the effect of probiotic cultures added to commercial yogurt over a known population of listeria monocytogenes and escherichia coli o157:h7]. | the effect of probiotic cultures over listeria monocytogenes and escherichia coli o157:h7 during yogurt storage was evaluated. two different yogurt brands, one with additional probiotic cultures (lactobacillus casei and l. acidophilus) were inoculated with known populations (106 ufc/g) of either l. monocytogenes or e. coli o157:h7 in three different times and stored for 32 days at 5 degrees c. every four days the count of lactic bacteria, the added pathogens and ph was evaluated, according to th ... | 2004 | 15807204 |
| reduction of listeria monocytogenes populations during exposure to a simulated gastric fluid following storage of inoculated frankfurters formulated and treated with preservatives. | the effect of a simulated gastric fluid (adjusted to ph 1.0 with hcl) on listeria monocytogenes, inoculated postprocessing on pork frankfurters formulated with sodium lactate (sl) and sodium diacetate (sd) and not dipped or dipped in solutions of lactic acid or acetic acid, was evaluated during storage of the frankfurters at 10 degrees c for 40 days. pork frankfurters containing 1.8% sl, 0.25% sd, 1.8% sl+0.125% sd, or 1.8% sl+0.25% sd were inoculated with 10(2)-10(3) cfu/cm2 of a 10-strain prep ... | 2005 | 15808365 |
| quantitative detection of listeria monocytogenes in biofilms by real-time pcr. | a quantitative method based on a real-time pcr assay to enumerate listeria monocytogenes in biofilms was developed. the specificity for l. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a sybr green i real-time pcr assay. the number of l. monocytogenes detected growing in biofilms was 6 x 10(2) cfu/cm2. | 2005 | 15812058 |
| human dendritic cells process and present listeria antigens for in vitro priming of autologous cd4+ t lymphocytes. | the role of human dendritic cells (dc) in the immune response toward intracellularly growing listeria was analyzed under in vitro conditions using several morphological and functional methods. dc incubated with listeria innocua and l. monocytogenes, respectively, readily phagocytosed the bacteria. listeria did not impair viability and immunogenic potential of human dc. listerial antigens were found to be processed within the lysosomal compartment of dc and colocalized with major histocompatibili ... | 2005 | 15812647 |
| translation elongation factor ef-tu is a target for stp, a serine-threonine phosphatase involved in virulence of listeria monocytogenes. | listeria monocytogenes is a pathogen that causes listeriosis, a severe food-borne infection. this bacterium, in order to survive and grow in the multiple conditions encountered in the host and the environment, has evolved a large number of regulatory elements, in particular many signal transduction systems based on reversible phosphorylation. the genome sequence has revealed genes for 16 putative two-component systems, four putative tyrosine phosphatases, three putative serine-threonine kinases ... | 2005 | 15813732 |
| the mutation g145s in prfa, a key virulence regulator of listeria monocytogenes, increases dna-binding affinity by stabilizing the hth motif. | listeria monocytogenes, a gram-positive, facultative intracellular human pathogen, causes systemic infections with high mortality rate. the majority of the known pathogenicity factors of l. monocytogenes is regulated by a single transcription factor, prfa. hyperhaemolytic laboratory strains of l. monocytogenes express the constitutively active mutant prfa(g145s) inducing virulence gene overexpression independent of environmental conditions. prfa belongs to the crp/fnr family of transcription fac ... | 2005 | 15813735 |
| ipr1 gene mediates innate immunity to tuberculosis. | an estimated eight million people are infected each year with the pathogen mycobacterium tuberculosis, and more than two million die annually. yet only about 10% of those infected develop tuberculosis. genetic variation within host populations is known to be significant in humans and animals, but the nature of genetic control of host resistance to tuberculosis remains poorly understood. previously we mapped a new genetic locus on mouse chromosome 1, designated sst1 (for supersusceptibility to tu ... | 2005 | 15815631 |
| engineered e. coli delivers therapeutic genes to the colonic mucosa. | taking advantage of the proximity of bowel mucosa to luminal bacteria, we have attempted to deliver a therapeutic gene to the colonic mucosa by oral administration of an invasive and non-pathogenic escherichia coli. e. coli diamenopimelate (dap) auxotroph, harboring plasmid pgb2omegainv-hly, express the inv gene from yersinia pseudotubercolosis that confers the ability to invade nonprofessional phagocytic cells and the hly gene from listeria monocytogenes that allows expression of lystreriolysin ... | 2005 | 15815705 |
| distinct in vivo dendritic cell activation by live versus killed listeria monocytogenes. | immunization of mice with live or heat-killed listeria monocytogenes (hklm) efficiently primes pathogen-specific cd8(+) t cells. t lymphocytes primed by hklm, however, undergo attenuated proliferation and do not fully differentiate. thus, only infection with live bacteria induces long-term, cd8(+) t cell-mediated protective immunity. in this study we demonstrate that live and heat-killed bacteria, while both associating with mac-3(+)cd11b(hi) cells, localize to distinct splenic areas following i ... | 2005 | 15816001 |
| identification of the gene encoding a 38-kilodalton immunogenic and protective antigen of streptococcus suis. | in our continued effort to search for a streptococcus suis protein(s) that can serve as a vaccine candidate or a diagnostic reagent, we constructed and screened a gene library with a polyclonal antibody raised against the whole-cell protein of s. suis type 2. a clone that reacted with the antibody was identified and characterized. analysis revealed that the gene encoding the protein is localized within a 2.0-kbp ecori dna fragment. the nucleotide sequence contained an open reading frame that enc ... | 2005 | 15817754 |
| murine pulmonary infection with listeria monocytogenes: differential susceptibility of balb/c, c57bl/6 and dba/2 mice. | murine listeriosis is a paradigm to understand host pathogen interactions. airway infections with listeria monocytogenes, although representing a serious problem in early onset neonatal listeriosis, has not been investigated in detail in animal models so far. here, the susceptibility of balb/c, dba/2 and c57bl/6 mice towards an intratracheal (i.t.) infection with virulent l. monocytogenes egde and the attenuated variant l. monocytogenes egd hlyw491a(perl3-cmvgfp) is reported. the course of infec ... | 2005 | 15820148 |
| multistate outbreak of listeria monocytogenes infection linked to delicatessen turkey meat. | despite a decreasing incidence of listeriosis in the united states, molecular subtyping has increased the number of recognized outbreaks. in september 2000, the new york city department of health identified a cluster of infections caused by listeria monocytogenes isolates with identical molecular subtypes by pulsed-field gel electrophoresis (pfge) and ribotyping. | 2005 | 15824987 |
| gastroenteritis due to listeria monocytogenes. | it has been known for a long time that many patients experience diarrhea antecedent to the development of bacteremia or meningoencephalitis due to listeria monocytogenes, but it was only recently that convincing evidence was obtained that this organism can cause acute, self-limited, febrile gastroenteritis in healthy persons. at least 7 outbreaks of foodborne gastroenteritis due to l. monocytogenes have been reported. illness typically occurs 24 h after ingestion of a large inoculum of bacteria ... | 2005 | 15825036 |
| susceptibility of clinical isolates of bacteria to cefoxitin and cephalothin. | the susceptibility of 4,929 unselected clinical isolates of bacteria to cefoxitin and cephalothin was determined by the single-disk method, using a computer-associated electronic zone analyzer to obtain, record, and process measurements of sizes of zones of inhibition. both cefoxitin and cephalothin were effective against most gram-positive strains, including staphylococcus aureus, s. epidermidis, micrococci, and all streptococci except enterococci. the three strains of listeria monocytogenes te ... | 1974 | 15830480 |
| thermal resistance of salmonella enterica serotypes, listeria monocytogenes, and staphylococcus aureus in high solids liquid egg mixes. | decimal reduction times (d-values) were determined for salmonella enterica serotypes, listeria monocytogenes, and staphylococcus aureus in two high solids egg mixes designated a and b (water activity [a(w)] = 0.76 and 0.82; solids = 53.12 and 52.63%; ph = 5.09 and 5.29; viscosity = 183 and 119 centipoise/s, respectively) using a low-volume (0.06 ml) sealed glass capillary tube procedure. for salmonella, d-values ranged from 0.035 (70 degrees c) to 0.193 min (64 degrees c) in product a and from 0 ... | 2005 | 15830659 |
| development of a surface plasmon resonance-based immunoassay for listeria monocytogenes. | a polyclonal antibody was produced against internalin b (inlb)-enriched extract and used to develop an inhibition assay to detect listeria monocytogenes cells in solution using surface plasmon resonance. the gene sequence encoding for the inlb protein was cloned into a qiagen pqe-60 vector, expressed in escherichia coli, and purified by immobilized metal affinity chromatography. protein g-purified anti-inlb-enriched extract polyclonal antibody was incubated with various concentrations of l. mono ... | 2005 | 15830663 |
| predicting heat inactivation of listeria monocytogenes under nonisothermal treatments. | the aim of this study was to find a model that accurately predicts the heat inactivation of listeria monocytogenes (atcc 15313) at constantly rising heating rates (0.5 to 9 degrees c/min) in media of different ph values (4.0 to 7.4). survival curves of l. monocytogenes obtained under isothermal treatments at any temperature were nearly linear. estimations of survival curves under nonisothermal treatments obtained from heat resistance parameters of isothermal treatments adequately fit experimenta ... | 2005 | 15830664 |
| inactivation of barotolerant listeria monocytogenes in sausage by combination of high-pressure processing and food-grade additives. | food-grade additives were used to enhance the efficacy of high-pressure processing (hpp) against barotolerant listeria monocytogenes. three strains of l. monocytogenes (scott a, osy-8578, and osy-328) were compared for their sensitivity to hpp, nisin, tert-butylhydroquinone (tbhq), and their combination. inactivation of these strains was evaluated in 0.2 m sodium phosphate buffer (ph 7.0) and commercially sterile sausage. a cell suspension of l. monocytogenes in buffer (10(9) cfu/ml) was treated ... | 2005 | 15830665 |
| monochloramine versus sodium hypochlorite as antimicrobial agents for reducing populations of bacteria on broiler chicken carcasses. | studies were conducted to compare the effect of sodium hypochlorite (sh) versus monochloramine (mon) on bacterial populations associated with broiler chicken carcasses. in study 1, nominal populations (6.5 to 7.5 log cfu) of escherichia coli, listeria monocytogenes, pseudomonas fluorescens, salmonella serovars, shewanella putrefaciens, and staphylococcus aureus were exposed to sterilized chiller water (controls) or sterilized chiller water containing 50 ppm sh or mon. sh at 50 ppm eliminated all ... | 2005 | 15830667 |
| fate of listeria monocytogenes inoculated onto the surface of model turkey frankfurter pieces treated with zein coatings containing nisin, sodium diacetate, and sodium lactate at 4 degrees c. | the antimicrobial effects of zein coatings containing nisin, sodium lactate, and sodium diacetate against listeria monocytogenes on turkey frankfurters at 4 degrees c were determined. our objectives were to determine whether zein, nisin, lactate, and diacetate alone or in combination could control the growth of l. monocytogenes on full-fat turkey frankfurters at 4 degrees c and to determine whether lactate or diacetate had any synergistic effect on the activity of nisin. turkey frankfurter piece ... | 2005 | 15830684 |
| effectiveness of trisodium phosphate treatment against pathogenic and spoilage bacteria on poultry during refrigerated storage. | to determine the efficacy of trisodium phosphate (tsp) against pathogenic and spoilage bacteria on poultry and to assess the influence of the bacterial combination of inoculum on tsp effect, chicken legs were coinoculated with similar concentrations of a pathogenic (salmonella enterica ser. enteritidis or listeria monocytogenes) and a spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were not treated (control). microbiological a ... | 2005 | 15830686 |
| the role of sigmab in the stress response of gram-positive bacteria -- targets for food preservation and safety. | the alternative sigma factor sigmab modulates the stress response of several gram-positive bacteria, including bacillus subtilis and the food-borne human pathogens bacillus cereus, listeria monocytogenes and staphylococcus aureus. in all these bacteria, sigmab is responsible for the transcription of genes that can confer stress resistance to the vegetative cell. recent findings indicate that sigmab also plays an important role in antibiotic resistance, pathogenesis and cellular differentiation p ... | 2005 | 15831390 |
| cytosolic delivery of viral nucleoprotein by listeriolysin o-liposome induces enhanced specific cytotoxic t lymphocyte response and protective immunity. | cytotoxic t lymphocytes (ctls) are capable of conferring protection against intracellular pathogens and tumor. protective antiviral immunity, mediated by the activation of antigenic epitope-specific ctl, can be achieved by delivering exogenous antigen into the cytosol of antigen-presenting cells. cytosolic introduction of vaccine antigen, however, requires a specialized delivery strategy due to the membrane barrier limiting the access of macromolecules to the cytosol. in this study, we have inve ... | 2004 | 15832496 |
| mode of action of piscicocin cs526 produced by carnobacterium piscicola cs526. | piscicocin cs526 is a unique class iia bacteriocin produced by carnobacterium piscicola cs526. the mode of action against the sensitive strain listeria monocytogenes iid581 was evaluated. | 2005 | 15836484 |
| characterization of a bacteriocin produced by enterococcus faecium gm-1 isolated from an infant. | to partially characterize the bacteriocin produced by the gm-1 strain of enterococcus faecium, isolated from the faeces of a newborn human infant. | 2005 | 15836487 |
| inactivation of escherichia coli, listeria monocytogenes, pseudomonas aeruginosa and staphylococcus aureus on stainless steel and glass surfaces by neutral electrolysed water. | to ascertain the efficacy of neutral electrolysed water (new) in reducing escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and listeria monocytogenes on glass and stainless steel surfaces. its effectiveness for that purpose is compared with that of a sodium hypochlorite (naclo) solution with similar ph, oxidation-reduction potential (orp) and active chlorine content. | 2005 | 15836736 |
| specific molecular detection of carnobacterium piscicola sf668 in cold smoked salmon. | to establish a rapid and reliable multiplex pcr (mpcr)-based method allowing specific identification of carnobacterium piscicola sf668 during storage of cold smoked salmon (css). | 2005 | 15836740 |
| the listeria monocytogenes prfap2 promoter is regulated by sigma b in a growth phase dependent manner. | listeria monocytogenes prfa, encoding positive regulatory factor a, is transcribed from three promoters (prfap1, prfap2, and pplca). the prfap2 promoter was previously proposed to be sigma b (sigma(b))-dependent. this hypothesis was tested by creating prfa promoter-gus transcriptional fusions in both l. monocytogenes wild-type (wt) and deltasigb backgrounds and then measuring (i) beta-glucuronidase (gus) activities; (ii) gus mrna transcript levels; and (iii) the presence or absence of gus in cel ... | 2005 | 15837390 |
| comparative proteome analysis of secretory proteins from pathogenic and nonpathogenic listeria species. | extracellular proteins of bacterial pathogens play a crucial role in the infection of the host. here we present the first comprehensive validation of the secretory subproteome of the gram positive pathogen listeria monocytogenes using predictive bioinformatic and experimental proteomic approaches. the previous original signal peptide (sp) prediction (glaser et al., science 2001, 294, 849-852) has been greatly improved by an in-depth analysis using seven different bioinformatic tools. subsequent ... | 2005 | 15838904 |
| bacterial delivery of functional messenger rna to mammalian cells. | the limited access to the nuclear compartment may constitute one of the major barriers after bacteria-mediated expression plasmid dna delivery to eukaryotic cells. alternatively, a self-destructing listeria monocytogenes strain was used to release translation-competent mrna directly into the cytosol of epithelial cells, macrophages and human dendritic cells. enhanced green fluorescent protein (egfp)-encoding mrna, adapted for translation in mammalian cells by linking an ires element to the 5'-en ... | 2005 | 15839900 |
| reducing the stimulation of cd8+ t cells during infection with intracellular bacteria promotes differentiation primarily into a central (cd62lhighcd44high) subset. | during infection with lymphocytic choriomeningitis virus, cd8(+) t cells differentiate rapidly into effectors (cd62l(low)cd44(high)) that differentiate further into the central memory phenotype (cd62l(high)cd44(high)) gradually. to evaluate whether this cd8(+) t cell differentiation program operates in all infection models, we evaluated cd8(+) t cell differentiation during infection of mice with recombinant intracellular bacteria, listeria monocytogenes (lm) and mycobacterium bovis (bcg), expres ... | 2005 | 15843531 |
| impact of antimicrobial ingredients and irradiation on the survival of listeria monocytogenes and the quality of ready-to-eat turkey ham. | irradiation is an effective technology in eliminating listeria monocytogenes, but it induces quality changes in meat products at or above specific radiation doses. to minimize irradiation-induced quality changes, only low irradiation doses are recommended. however, low-dose irradiation provides a chance for some pathogens to survive and proliferate during prolonged storage. to solve this problem, antimicrobial ingredients [2% sodium lactate (sl), 0.1% sodium diacetate (sda), 0.1% potassium benzo ... | 2005 | 15844819 |
| listeria monocytogenes skin infection with cerebritis and haemophagocytosis syndrome in a bone marrow transplant recipient. | in this report, we describe unusual and unreported manifestations of listeria monocytogenes infection in a bone marrow transplant recipient, including cutaneous infection with an hamophagocytosis syndrome and cerebritis. l. monocytogenes occurred despite a broad spectrum antibiotherapy. l. monocytogenes was isolated from a skin biopsy. outcome was favorable with amoxicillin and gentamicin therapy. l. monocytogenes infection should be suspected in patients with cerebritis despite large spectrum a ... | 2005 | 15845437 |
| molecular cloning and characterization of three beta-defensins from canine testes. | mammalian beta-defensins are small cationic peptides possessing broad antimicrobial and physiological activities. because dogs are particularly resilient to sexually transmitted diseases, it has been proposed that their antimicrobial peptide repertoire might provide insight into novel antimicrobial therapeutics and treatment regimens. to investigate this proposal, we cloned the full-length cdna of three canine beta-defensin isoforms (cbd-1, -2, and -3) from canine testicular tissues. their predi ... | 2005 | 15845463 |