Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| prevalence and typing of listeria monocytogenes in ready-to-eat food products on the belgian market. | listeria monocytogenes is a major concern to producers of ready-to-eat foods because of the high mortality rate associated with listeriosis and the widespread nature of the organism. to investigate the prevalence of this pathogen in different ready-to-eat food products on the belgian market, a variety of 252 ready-to-eat food products, mainly fish and meat products, were analyzed. overall, l. monocytogenes was detected in 23.4% of the samples. the highest prevalence of l. monocytogenes was found ... | 2004 | 15553631 |
| strain-specific differences in the attachment of listeria monocytogenes to alfalfa sprouts. | contamination of fresh produce with listeria monocytogenes has resulted in outbreaks of systemic listeriosis and febrile gastroenteritis. recalls of alfalfa sprouts have occurred due to contamination with l. monocytogenes. alfalfa sprouts were used as a preharvest model to study the interaction with this human pathogen. seventeen strains were assessed for their capacity to colonize alfalfa sprouts, and strain-specific differences (not related to source, serotype, or lineage) were revealed when t ... | 2004 | 15553632 |
| dairy farm reservoir of listeria monocytogenes sporadic and epidemic strains. | identifying the reservoirs of a pathogen is vital for control of sporadic disease and epidemics. listeria monocytogenes is a zoonotic foodborne pathogen that is responsible for 28% of food-related deaths in the united states annually, as well as a major cause of massive product recalls worldwide. to examine the role of the dairy farm as a potential source or reservoir for l. monocytogenes subtypes shown to cause human listeriosis, we compared the pulsed-field gel electrophoresis (pfge) restricti ... | 2004 | 15553633 |
| longitudinal studies on listeria in smoked fish plants: impact of intervention strategies on contamination patterns. | four ready-to-eat smoked fish plants were monitored for 2 years to study listeria contamination patterns and the impact of plant-specific listeria control strategies, including employee training and targeted sanitation procedures, on listeria contamination patterns. samples from the processing plant environment and from raw and finished product were collected monthly and tested for listeria spp. and listeria monocytogenes. before implementation of intervention strategies, 19.2% of raw product sa ... | 2004 | 15553634 |
| efficacy of acidic electrolyzed water ice for pathogen control on lettuce. | acidic electrolyzed water (acew) was used as frozen acew (acew-ice) for inactivation of listeria monocytogenes and escherichia coli o157:h7 on lettuce. acew-ice was prepared from acew with 20, 50, 100, and 200 ppm of available chlorine by freezing at -40 degrees c and generated 30, 70, 150, and 240 ppm of chlorine gas (cl2), respectively. the acew-ice was placed into styrene-foam containers with lettuce samples at 20 degrees c for 24 h. although acew-ice generating 30 ppm cl2 had no effect on l. ... | 2004 | 15553639 |
| antibacterial effect of water-soluble tea extracts on foodborne pathogens in laboratory medium and in a food model. | the microbial inhibition of foodborne pathogens was determined in brain heart infusion broth with 10% (wt/vol) water-soluble extracts of green, jasmine, black, dungglre, and oolong tea against escherichia coli o157:h7, salmonella enterica serovar enteritidis, listeria monocytogenes, and staphylococcus aureus. the mixed culture (approximately 6.0 log cfu/ml), which was composed of the four pathogens, was inoculated into brain heart infusion broth with and without tea extracts. after incubation at ... | 2004 | 15553649 |
| shortening the infectious period does not alter expansion of cd8 t cells but diminishes their capacity to differentiate into memory cells. | following a primary immune response, a portion of effector t cells gives rise to long-lived memory cells. although primary expansion and differentiation of effector cd8 t cells is dictated by a brief exposure to ag, it is unclear whether full memory differentiation is also programmed within the same short window. by carefully modulating the kinetics of listeria monocytogenes infection, we analyzed the requirements for the programming of effector and memory t cell development in vivo. we find tha ... | 2004 | 15557161 |
| immunostimulating properties of intragastrically administered acetobacter-derived soluble branched (1,4)-beta-d-glucans decrease murine susceptibility to listeria monocytogenes. | we previously found that ac-1, an extracellular polysaccharide, produced by acetobacter xylinum and composed of (1,4)-beta-d-glucan with branches of glucosyl residues, showed a strong activity to induce production of interleukin-12 (il-12) p40 and tumor necrosis factor alpha by macrophages in vitro via toll-like receptor 4 (tlr-4) signaling. in the present study, we examined the effect of oral administration of ac-1 on protective immunity against listeria monocytogenes. mice were given ac-1 or p ... | 2004 | 15557623 |
| listeria monocytogenes sigmab contributes to invasion of human intestinal epithelial cells. | the role of sigma(b) in listeria monocytogenes infection of human intestinal epithelial cells was investigated. invasion defects associated with loss of sigma(b) paralleled those of a deltainla strain independently of the sigma(b)-dependent p2(prfa) promoter. concomitantly, amounts of inla transcript and inla protein were significantly decreased in the deltasigb strain. | 2004 | 15557671 |
| effect of environmental factors on the calibration curve (od-cfu) of listeria monocytogenes. | 2004 | 15560205 | |
| in silico reconstitution of listeria propulsion exhibits nano-saltation. | to understand how the actin-polymerization-mediated movements in cells emerge from myriad individual protein-protein interactions, we developed a computational model of listeria monocytogenes propulsion that explicitly simulates a large number of monomer-scale biochemical and mechanical interactions. the literature on actin networks and l. monocytogenes motility provides the foundation for a realistic mathematical/computer simulation, because most of the key rate constants governing actin networ ... | 2004 | 15562315 |
| listeria monocytogenes in products of animal origin in turkey. | a study was carried out on 430 samples of different foodstuffs (soft cheese, raw chicken, minced beef, sausage, fish) and 400 carcase samples (sheep, young and adult cattle) for screening of listeria monocytogenes. it was found that only one of the samples contained l. monocytogenes at > 10(3) cfu/ml in the initial examination, but another 42 samples contained l. monocytogenes following an enrichment process. l. monocytogenes was isolated most frequently from raw chicken samples (18%), but was n ... | 2004 | 15563103 |
| humoral and delayed-type hypersensitive responses against listeria monocytogenes phosphatidylinositol-specific phospholipase c in experimentally infected buffaloes. | the kinetics of antibody production against phosphatidylinositol-specific phospholipase c (pi-plc) and the isolation pattern of listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic l. monocytogenes. antibodies to pi-plc appeared by 4-8 days post infection (pi), with a peak between days 7 and 16 pi, when tested by indirect plate-elisa. subsequently, antibody titres in all the animals declined and became ... | 2004 | 15563104 |
| ifn regulatory factor 3-dependent induction of type i ifns by intracellular bacteria is mediated by a tlr- and nod2-independent mechanism. | like viruses, intracellular bacteria stimulate their host cells to produce type i ifns (ifn-alpha and ifn-beta). in our study, we investigated the signals and molecules relevant for the synthesis of and response to ifn by mouse macrophages infected with listeria monocytogenes. we report that ifn-beta is the critical immediate-early ifn made during infection, because the synthesis of all other type i ifn, expression of a subset of infection-induced genes, and the biological response to type i ifn ... | 2004 | 15585867 |
| specific detection of cytopathogenic listeria monocytogenes using a two-step method of immunoseparation and cytotoxicity analysis. | the development of rapid methods for detection of viable listeria monocytogenes is crucial to prevent listeriosis and product recalls. while immunomagnetic separation has been used for isolating listeria spp., lack of specificity and pathogenicity determination render this method unsatisfactory. a two-step method using protein a agarose beads (immunobeads) coated with a more specific antibody, monoclonal antibody (mab)-c11e9 for l. monocytogenes was developed. immunobeads were allowed to capture ... | 2005 | 15590100 |
| [listeria monocytogenes endophthalmitis]. | listeria monocytogenes is a rare cause of endogenous endophthalmitis. controversy exists concerning clinical progress and prognosis in this bacterial infection. however, only a few reports about the clinical features have been previously published in the literature. | 2004 | 15599813 |
| recombinant listeria vaccines containing pest sequences are potent immune adjuvants for the tumor-associated antigen human papillomavirus-16 e7. | previous work in our laboratory has established that the fusion of tumor-associated antigens to a truncated form of the listeria monocytogenes virulence factor listeriolysin o (llo) enhances the immunogenicity and antitumor efficacy of the tumor antigen when delivered by listeria or by vaccinia. llo contains a pest sequence at the nh(2) terminus. these sequences, which are found in eukaryotic proteins with a short cellular half-life, target proteins for degradation in the ubiquitin-proteosome pa ... | 2004 | 15604239 |
| in vitro screening of potential probiotic activities of selected lactobacilli isolated from unpasteurized milk products for incorporation into soft cheese. | the aim was to select potentially probiotic lactobacilli from 88 strains isolated from unpasteurized milk and cheese products, and to incorporate these bacteria in a viable state into a soft cheese, without changing its quality. the survival of these bacteria was assessed in acidic and bile conditions, after freezing at -80 degrees c. four strains from unpasteurized camembert--two lactobacillus plantarum strains and two lb. paracasei/casei strains--were identified and typed by pcr and pfge and w ... | 2004 | 15605712 |
| listeria monocytogenes meningitis in an immunocompetent adult patient. | to report an interesting case of meningitis caused by listeria monocytogenes in an immunocompetent adult. | 2005 | 15608483 |
| [investigation of a listeriosis cluster: how complete are laboratory-based reports?]. | in germany, listeriosis surveillance is based on mandatory laboratory notification of listeria monocytogenes. local public health departments (lphds) forward the received reports anonymously to state public health departments (sphds) which send them to the national public health centre. for the 28th reporting week (rw) 2003, lphds reported four cases of listeriosis to the sphd of hesse, cumulating to 19 listeriosis cases in 2003 compared to four cases in rw 1 - 28, 2002. | 2004 | 15609211 |
| lyophilized preparations of bacteriocinogenic lactobacillus curvatus and lactococcus lactis subsp. lactis as potential protective adjuncts to control listeria monocytogenes in dry-fermented sausages. | study of the effectiveness of in situ bacteriocin production by lactic acid bacteria (lab) to control listeria monocytogenes in dry-fermented sausages. | 2005 | 15610417 |
| growth inhibition of listeria monocytogenes by a nonbacteriocinogenic carnobacterium piscicola. | this study elucidates the mechanisms by which a nonbacteriocinogenic carnobacterium piscicola inhibits growth of listeria monocytogenes. | 2005 | 15610430 |
| encephalitic listeriosis in ruminants: immunohistochemistry as a diagnostic tool. | a retrospective analysis of 42 ruminants (sheep, goats and cattle) with suspected meningo-encephalitis was performed. the clinical findings and the post-mortem results of the animals have been specified. bacteriological culture, gram's stain and listeria-specific immunohistochemistry were performed in order to confirm the diagnosis of these cases. the results of the different methods were evaluated for the detection of listerial antigens. bacteriological culture was positive in 28.5% of the case ... | 2004 | 15610491 |
| chemical compositions and antibacterial effects of essential oils of turkish oregano (origanum minutiflorum), bay laurel (laurus nobilis), spanish lavender (lavandula stoechas l.), and fennel (foeniculum vulgare) on common foodborne pathogens. | chemical compositions and inhibitory effects of essential oils of turkish oregano (origanum minutiflorum o. schwarz & p. h. davis), bay laurel (laurus nobilis l.), spanish lavender (lavandula stoechas subsp. stoechas l.), and fennel (foeniculum vulgare mill.) on escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and staphylococcus aureus were determined. after the essential oils were applied on the foodborne pathogens at doses of 0 (control), 5, 10, 20, 30, 40, 50, and 80 ... | 2004 | 15612826 |
| essential oils of satureja, origanum, and thymus species: chemical composition and antibacterial activities against foodborne pathogens. | the chemical composition of the essential oils obtained from the species restricted to greece and the eastern mediterranean region, satureja spinosa l. and thymus longicaulis l.; species endemic to central and south greece, satureja parnassica ssp. parnassica heldr. and sart ex boiss.; species endemic to the island of crete, origanum dictamnus l.; and species widely distributed in the mediterranean region, satureja thymbra l. and origanum vulgare l. subsp. hirtum, were determined by gas chromato ... | 2004 | 15612827 |
| mechanistic study of membrane concentration and recovery of listeria monocytogenes. | detection of the foodborne pathogen listeria monocytogenes requires that food samples be processed to remove proteins and lipids, concentrate microorganisms to a detectable concentration, and recover the concentrated cells in a small volume compatible with micron-scale biochips. mechanistic considerations addressed in this research include the roles of membrane structure, pore size, and detergents in maximizing recovery of cells from a complex biological fluid. the fluid in this case was a food ... | 2005 | 15614853 |
| [initial antibiotic therapy of neonatal sepsis]. | it is certain that in the past the types of bacterial agents responsible for neonatal sepsis and their sensitivity to antibiotics were not the same in all historical periods. however, the reports confirming the conclusion have been published only in the last three years. according to these facts, the bacterial causes of neonatal sepsis were analyzed in patients treated at the university children's hospital in belgrade (s&m) as well as their sensitivity to antibiotics to determine the most effect ... | 2004 | 15615466 |
| impairment of host resistance to listeria monocytogenes infection in liver of db/db and ob/ob mice. | leptin is an adipocyte-derived hormone that regulates a number of physiological functions, including energy homeostasis and immune function. in immune responses, leptin plays a role in the induction of inflammation. we investigated a role of leptin in listeria monocytogenes infection using leptin receptor-deficient db/db mice and leptin-deficient ob/ob mice. these mutant mice were highly susceptible to l. monocytogenes, and the elimination of bacteria from the liver was inhibited. after infectio ... | 2005 | 15616027 |
| salmonella-mediated oral dna vaccination using stabilized eukaryotic expression plasmids. | the use of salmonella for the delivery of plasmid-encoded heterologous antigens to eukaryotic host cells has proven successful in experimental systems, but its general applicability is still hampered by a severe instability of transformants carrying these expression plasmids. to overcome the problem of plasmid instability, new low copy number expression plasmids were constructed using different replicons. comparative studies between transformants of the high copy number plasmid pcmvbeta and the ... | 2005 | 15616604 |
| salmonella pathogenicity island 2-mediated overexpression of chimeric ssph2 proteins for simultaneous induction of antigen-specific cd4 and cd8 t cells. | salmonella enterica serovar typhimurium employs two different type iii secretion systems (ttss) encoded within salmonella pathogenicity islands 1 and 2 (spi1 and spi2) for targeting of effector proteins into the cytosol of eukaryotic cells during different stages of the infection cycle. the spi1 ttss translocates virulence factors across the plasma membrane when the bacterium initially contacts the host cell. in contrast, the spi2 ttss functions to translocate proteins across the membrane of the ... | 2005 | 15618170 |
| truncated internalin a and asymptomatic listeria monocytogenes carriage: in vivo investigation by allelic exchange. | allelic exchange of the region coding for the c terminus of inla between one epidemic (with an 80-kda inla) and one asymptomatic (with a 47-kda inla) carriage listeria monocytogenes strain confirmed the need for this region for internalin entry in vitro. interestingly, restoration of internalin a functionality did not result in full virulence in chicken embryo assays. | 2005 | 15618209 |
| differential protein expression by porphyromonas gingivalis in response to secreted epithelial cell components. | the human oral pathogen porphyromonas gingivalis colonizes the gingival crevice and invades gingival epithelial cells. multidimensional capillary high-performance liquid chromatography coupled with tandem mass spectrometry and two-dimensional gel electrophoresis were used to analyze the proteome of p. gingivalis as it adapts to a set of experimental conditions designed to reflect important features of an epithelial cell environment. 1014 proteins (46% of the total theoretical proteome) were iden ... | 2005 | 15619293 |
| listeria's right-handed helical rocket-tail trajectories: mechanistic implications for force generation in actin-based motility. | listeria monocytogenes forms right-handed helical rocket tail trajectories during actin-based motility in cell-free extracts, and this stereochemical feature is consistent with actoclampin's affinity-modulated, clamped-filament elongation model [dickinson and purich, 2002: biophys j 82:605-617]. in that mechanism, right-handed torque is generated by an end-tracking molecular motor, each comprised of a filament barbed end and clamping protein that processively traces the right-handed helix of its ... | 2005 | 15627275 |
| analysis of the listeria cell wall proteome by two-dimensional nanoliquid chromatography coupled to mass spectrometry. | genome analyses have revealed that the gram-positive bacterial species listeria monocytogenes and l. innocua contain a large number of genes encoding surface proteins predicted to be covalently bound to the cell wall (41 and 34, respectively). the function of most of these proteins is unknown and they have not even been identified biochemically. here, we report the first characterization of the listeria cell wall proteome using a nonelectrophoretic approach. the material analyzed consisted of a ... | 2005 | 15627966 |
| sigb-dependent in vitro transcription of prfa and some newly identified genes of listeria monocytogenes whose expression is affected by prfa in vivo. | recent studies have identified several new genes in listeria monocytogenes which are positively or negatively affected by prfa and grouped into three classes (e. milohanic et al., mol. microbiol. 47:1613-1625, 2003). in vitro transcription performed with promoters of some class iii genes showed strict sigb-dependent but prfa-independent transcription initiation. transcription starting at the prfa promoter pprfa2 was also optimal with sigb-loaded rna polymerase, suggesting a direct link between s ... | 2005 | 15629954 |
| mislocalization or reduced expression of arf gtpase-activating protein asap1 inhibits cell spreading and migration by influencing arf1 gtpase cycling. | adp-ribosylation factor (arf) family of small gtp-binding proteins plays a central role in membrane trafficking and cytoskeletal remodeling. asap1 (arf-gap containing sh3, ankyrin repeats, and ph domain) is a phospholipid-dependent arf gtpase-activating protein (arf-gap) that binds to protein-tyrosine kinases src and focal adhesion kinase. using affinity chromatography and mass spectrometry (ms), we identified the adaptor protein cd2-associated protein (cd2ap) as a candidate binding partner of a ... | 2005 | 15632162 |
| omega-3 polyunsaturated fatty acids impair in vivo interferon- gamma responsiveness via diminished receptor signaling. | a high intake of omega-3 polyunsaturated fatty acids (n-3 pufas) in mice causes impaired host resistance to listeria monocytogenes. we wished to determine the role of interferon (ifn)-gamma signaling in this increased disease susceptibility. | 2004 | 15633108 |
| temperature and biological soil effects on the survival of selected foodborne pathogens on a mortar surface. | the survival of three foodborne pathogens (listeria monocytogenes, yersinia enterocolitica, and salmonella) attached to mortar surfaces, with or without biological soil (porcine serum) and incubated at either 4 or 10 degrees c in the presence of condensate, was evaluated. soiled and unsoiled coupons were inoculated by immersion into a five-strain cocktail (approximately 10(7) cfu/ml) of each organism type and evaluated. coupons were incubated at 25 degrees c for 2 h to allow attachment of cells, ... | 2004 | 15633668 |
| surface material, temperature, and soil effects on the survival of selected foodborne pathogens in the presence of condensate. | the effects of surface type (stainless steel, acetal resin, and fiberglass reinforced plastic wall paneling [frp]), soil, and temperature on the survival of listeria monocytogenes, salmonella spp., and yersinia enterocolitica, in the presence of condensate were evaluated. surface coupons--half soiled with sterile porcine serum--were exposed to cell suspensions made from individual five-strain cocktails composed of organisms from the same genus (10(7) cfu/ml) in butterfield's phosphate buffer and ... | 2004 | 15633669 |
| mathematical model of listeria monocytogenes cross-contamination in a fish processing plant. | listeriosis is a foodborne disease caused by the bacterium listeria monocytogenes. the food industry and government agencies devote considerable resources to reducing contamination of ready-to-eat foods with l. monocytogenes. because inactivation treatments can effectively eliminate l. monocytogenes present on raw materials, postprocessing cross-contamination from the processing plant environment appears to be responsible for most l. monocytogenes food contamination events. an improved understan ... | 2004 | 15633673 |
| survival of listeria monocytogenes during storage of ready-to-eat meat products processed by drying, fermentation, and/or smoking. | the survival of listeria monocytogenes was evaluated on 15 ready-to-eat meat products made using drying, fermentation, and/or smoking. the products were obtained from six processors and included summer sausage, smoked cured beef, beef jerky, snack stick, and pork rind and crackling products. the water activity of the products ranged from 0.27 (pork rinds and cracklings) to 0.98 (smoked cured beef slices). products were inoculated with a five-strain cocktail of l. monocytogenes, repackaged under ... | 2004 | 15633674 |
| effect of single or sequential hot water and lactic acid decontamination treatments on the survival and growth of listeria monocytogenes and spoilage microflora during aerobic storage of fresh beef at 4, 10, and 25 degrees c. | the survival and growth of listeria monocytogenes and spoilage microflora during storage of fresh beef subjected to different decontamination treatments was studied. fresh beef inoculated with a five-strain mixture of l. monocytogenes (5.18 log cfu/cm2) was left untreated (control) or was immersed (30 s) in hot water (hw; 75 degrees c), 2% lactic acid (la; 55 degrees c), hot water followed by lactic acid (hw-la), or lactic acid followed by hot water (la-hw) and then stored aerobically at 4, 10, ... | 2004 | 15633675 |
| a predictive model for heat inactivation of listeria monocytogenes biofilm on stainless steel. | heat treatment of potential biofilm-forming sites is sometimes used for control of listeria monocytogenes in food processing plants. however, little information is available on the heat treatment required to kill l. monocytogenes present in biofilms. the purpose of this study was to develop a predictive model for the heat inactivation of l. monocytogenes in monoculture biofilms (strains scott a and 3990) and in biofilms with competing bacteria (pseudomonas sp. and pantoea agglomerans) formed on ... | 2004 | 15633676 |
| antibacterial activity of plants used in cooking for aroma and taste. | thirty-three plants used in cooking for aroma and taste were examined for antibacterial activity against pathogens causing foodborne infections. vibrio parahaemolyticus and staphylococcus aureus were sensitive to many kinds of plant extracts, whereas listeria monocytogenes, escherichia coli o157:h7, and salmonella enteritidis populations decreased in only six, one, and three plant extracts, respectively. the polyphenol content in the plants was significantly different between the antibacterial p ... | 2004 | 15633695 |
| t cells undergo rapid on/off but not on/off/on cycling of cytokine production in response to antigen. | inflammatory cytokines such as ifn-gamma and tnf produced by ag-stimulated cd4(+) and cd8(+) t cells are important in defense against microbial infection. however, production of these cytokines must be tightly regulated to prevent immunopathology. previous studies, conducted with balb/c mice, have suggested that 1) cd8(+) t cells maintain ifn-gamma production but transiently produce tnf in the continued presence of ag and 2) lymphocytic choriomeningitis virus-specific and in vitro-propagated eff ... | 2005 | 15634891 |
| cpg oligodeoxynucleotides enhance neonatal resistance to listeria infection. | infection by listeria monocytogenes causes serious morbidity and mortality during the neonatal period. previous studies established that immunostimulatory cpg oligodeoxynucleotides (odn) can increased the resistance of adult mice to many infectious pathogens, including listeria. this work examines the capacity of cpg odn to stimulate a protective immune response in newborns. results indicate that dendritic cells, macrophages, and b cells from 3-day-old mice respond to cpg stimulation by secretin ... | 2005 | 15634898 |
| design, nmr characterization and activity of a 21-residue peptide fragment of bacteriocin as-48 containing its putative membrane interacting region. | bacteriocin as-48 is a 70-residue cyclic polypeptide from enterococcus faecalis that shows a broad antimicrobial spectrum against both gram-positive and gram-negative bacteria. the structure of bacteriocin as-48 consists of a globular arrangement of five helices with a high positive electrostatic potential in the region comprising helix 4, the turn linking helix 4 and 5, and the n-terminus of helix 5. this region has been considered to participate in its biological activity and in particular in ... | 2005 | 15635724 |
| application of molecular genetic methods in diagnostics and epidemiology of food-borne bacterial pathogens. | salmonella enterica, campylobacter and yersinia species, shiga toxin-producing escherichia coli (stec), listeria monocytogenes and clostridium perfringens are the bacterial pathogens constituting the greatest burden of food-borne disease in finland. several molecular genetic methods have been applied to diagnose, discriminate and survey these bacteria. pcr, pcr-rflp and pfge are the most widely and successfully used. however, these methods are unable to replace conventional and internationally s ... | 2004 | 15638843 |
| [molecular basis of listeria monocytogenes fetoplacental tropism]. | 2005 | 15639011 | |
| synthesis, surface active and antimicrobial properties of new alkyl 2,6-dideoxy-l-arabino-hexopyranosides. | synthesis of alkyl 2,6-dideoxy-l-arabino-hexopyranosides was accomplished by the reaction of 1,5-anhydro-2,6-dideoxy-l-arabino-hex-1-enitol with fatty alcohols in dichloromethane, catalyzed by triphenylphosphine hydrobromide. reaction with octanol and dodecanol gave the corresponding alpha-glycosides in 50% and 42% yield, the beta-glycosides in 20% and 21% yield and the alpha-anomer of the ferrier product in 10% and 9% yield, respectively. deacetylation of the alpha-/beta-glycosides with sodium ... | 2005 | 15639239 |
| phosphoinositide 3-kinase is required for intracellular listeria monocytogenes actin-based motility and filopod formation. | motile nonmuscle cells concentrate phosphatidylinositol 3,4,5-trisphosphate (ptdins(3,4,5)p3) and phosphatidylinositol 4,5-bisphosphate (ptdins(4,5)p2) in areas of new actin filament assembly. there is great interest in assessing the in vivo functional significance of these phosphoinositides, and we have used listeria monocytogenes to explore the contribution of ptdins(3,4,5)p3 and ptdins(4,5)p2 to its actin-based motility. in listeria-infected ptk2 cells akt-pleckstrin homology (ph)-green fluor ... | 2005 | 15642729 |
| performance of a new chromogenic plating medium for the isolation of listeria monocytogenes from marine environments. | aims: this study investigated the performance of a new chromogenic plating medium for the detection of listeria monocytogenes from naturally contaminated samples obtained from marine environments in morocco in comparison with the conventional plating media palcam and oxford. methods: a total of 479 marine samples (sea water, sediment and mussels) were collected from 16 littoral sites in the region of agadir (western centre of morocco). they were examined for the presence of l. monocytogenes usin ... | 2005 | 15644105 |
| characterization of a mutant listeria monocytogenes strain expressing green fluorescent protein. | to construct a recombinant strain of listeria monocytogenes for the expression of heterologous genes, homologous recombination was utilized for insertional mutation, targeting its listeriolysin o gene (hly). the gene encoding green fluorescent protein (gfp) was used as the indicator of heterologous gene expression. the gene gfp was inserted into hly downstream from its promoter and signal sequence by an overlapping extension polymerase chain reaction, and was then cloned into the shuttle plasmid ... | 2005 | 15645077 |
| allergen immunotherapy with heat-killed listeria monocytogenes alleviates peanut and food-induced anaphylaxis in dogs. | heat-killed listeria monocytogenes (hkl) potently stimulates interferon (ifn)-gamma production in cd4 t-lymphocytes, and when used as adjuvant for immunotherapy, reduces immunoglobulin (ig)e production and reverses established allergen-induced airway hyperreactivity (ahr) in a murine model of asthma. we asked if such treatment could decrease established peanut-induced anaphylaxis or cow's milk-induced food allergy in highly food-allergic dogs. | 2005 | 15647048 |
| what is needed for effective antitumor immunotherapy? lessons learned using listeria monocytogenes as a live vector for hpv-associated tumors. | as a vaccine vector, listeria monocytogenes targets the innate immune system, resulting in a cytokine response that enhances antigen-presenting cell function as well as inducing a th1 profile. it also enhances cell-mediated immunity by targeting antigen delivery in antigen-presenting cells to both the mhc class i pathway of exogenous presentation that activates cd8 t cells and the mhc class ii pathway that processes antigen endogenously and presents it to cd4 t cells. in this review, we describe ... | 2005 | 15650885 |
| listeria monocytogenes-infected bone marrow myeloid cells promote bacterial invasion of the central nervous system. | listeria monocytogenes is a facultative intracellular pathogen that is able to invade the central nervous system causing meningoencephalitis and brain abscesses. the mechanisms allowing bacteria to cross the blood-brain barrier are poorly understood. in this work, we used an experimental model of acute listeriosis in the mouse inducing a reproducible invasion of the central nervous system. at the early phase of infection, we find that bacteria invade and rapidly grow in bone marrow cells identif ... | 2005 | 15659061 |
| land application of treated sewage sludge: quantifying pathogen risks from consumption of crops. | to predict the number of humans in the uk infected through consumption of root crops grown on agricultural land to which treated sewage sludge has been applied in accordance with the current regulations and guidance (safe sludge matrix). | 2005 | 15659193 |
| the svpa-srtb locus of listeria monocytogenes: fur-mediated iron regulation and effect on virulence. | in listeria monocytogenes the promoter region of the svpa-srtb locus contains a well-conserved fur box. we characterized the iron-regulation of this locus: real-time polymerase chain reaction analyses and anti-svpa immunoblots showed that, in response to iron deprivation svpa transcription and svpa production markedly increased (80-fold and 10-fold respectively), when initiated by either the addition of the iron chelator 2,2'-bipyridyl to bhi media, or by growth in iron-restricted minimal media. ... | 2005 | 15661014 |
| the actin slingshot. | actin polymerization generates the force that deforms the cell membrane, pulls the cell forward and propels endosomes and bacteria within the cell. the mechanism of force generation has been probed using experimental biomimetic systems where force generation and movement occur by the same actin-polymerization processes observed in cells. the advantage of such systems over living cells is that their physical properties can be changed, such as the size of the load, its composition and its deformab ... | 2005 | 15661520 |
| immune activation of type i ifns by listeria monocytogenes occurs independently of tlr4, tlr2, and receptor interacting protein 2 but involves tnfr-associated nf kappa b kinase-binding kinase 1. | type i ifns are well established antiviral cytokines that have also been shown to be induced by bacteria. however, the signaling mechanisms regulating the activation of these cytokines during bacterial infections remain poorly defined. we show that although gram-negative bacteria can activate the type i ifn pathway through tlr4, the intracellular gram-positive bacterium listeria monocytogenes (lm) can do so independently of tlr4 and tlr2. furthermore, experiments using genetic mutants and chemic ... | 2005 | 15661922 |
| contribution of three bile-associated loci, bsh, pva, and btlb, to gastrointestinal persistence and bile tolerance of listeria monocytogenes. | listeria monocytogenes must resist the deleterious actions of bile in order to infect and subsequently colonize the human gastrointestinal tract. the molecular mechanisms used by the bacterium to resist bile and the influence of bile on pathogenesis are as yet largely unexplored. this study describes the analysis of three genes--bsh, pva, and btlb--previously annotated as bile-associated loci in the sequenced l. monocytogenes egde genome (lmo2067, lmo0446, and lmo0754, respectively). analysis of ... | 2005 | 15664931 |
| pre-existing immunity to pathogenic listeria monocytogenes does not prevent induction of immune responses to feline immunodeficiency virus by a novel recombinant listeria monocytogenes vaccine. | listeria monocytogenes is an attractive biologic vaccine vector against hiv because it induces a strong cell mediated immune response, can be delivered by mucosal routes, can be readily manipulated to express viral antigens, and is easy and inexpensive to produce. proof of concept studies have been performed using hiv gag expressing recombinant l. monocytogenes in the mouse. here we report the development and validation of recombinant l. monocytogenes to be evaluated in the fiv/cat model of hiv. ... | 2005 | 15670884 |
| gelsolin mediates calcium-dependent disassembly of listeria actin tails. | the role of intracellular ca2+ in the regulation of actin filament assembly and disassembly has not been clearly defined. we show that reduction of intracellular free ca2+ concentration ([ca2+]i) to <40 nm in listeria monocytogenes-infected, egfp-actin-transfected madin-darby canine kidney cells results in a 3-fold lengthening of actin filament tails. this increase in tail length is the consequence of marked slowing of the actin filament disassembly rate, without a significant change in assembly ... | 2005 | 15671163 |
| [analysis of the immune resistance in an experimental murine model fed dietary lipids and infected with listeria monocytogenes]. | several dietary lipids are capable of exerting an immunosupressor effect. this action may have undiserable effects on the host immune resistance to infectious diseases. the purpose of the present study was to determinate the immune status of mice fed dietary lipids and experimentally infected with a virulent strain of listeria monocytogenes. balb/c mice were divided into four groups and were fed with their respective diet: low fat diet (lf, 20%), olive oil diet (oo, 20%), fish oil diet (fo, 20%) ... | 2004 | 15672648 |
| antibiotic susceptibility of listeria monocytogenes in denmark 1958-2001. | in order to see whether the susceptibility of danish listeria monocytogenes strains has changed over the years we examined a collection of human isolates from the period 1958-2001. we, furthermore, wanted to compare l. monocytogenes susceptibility testing using a disc diffusion assay with mic measurements performed by the e-test. 106 strains isolated predominantly from blood cultures and cerebrospinal fluids were examined together with three reference strains. susceptibility to the following ant ... | 2005 | 15676012 |
| [isolation of listeria monocytogenes from a patient with sealed ruptured thoracoabdominal aortic aneurysm]. | we are reporting a case of sealed rupture of thoracoabdominal aortic aneurysm associated with the isolation of listeria monocytogenes. the patient was a 75-year-old man with previous history of hypertension that had not required medication for the 3 years prior to hospital admission. he was admitted due to chest pain, but he was afebrile. there were no clinical findings indicating infection, although crp was slightly elevated. during his clinical course, a sealed rupture of a thoracoabdominal ao ... | 2004 | 15678977 |
| immunization with a tetramer derivative of an anti-inflammatory pentapeptide produced by entamoeba histolytica protects gerbils (meriones unguiculatus) against experimental amoebic abscess of the liver. | axenically grown entamoeba histolytica produces a pentapeptide (met-gln-cys-asn-ser) with several anti-inflammatory properties, including the inhibition of human monocyte locomotion (monocyte locomotion inhibitory factor (mlif)). a construct displays the same effects as the native material. it remains to be seen if mlif is used, or even produced in vivo by the tissue-invading parasite. if mlif were to be relevant in invasive amoebiasis, immunizing against it could diminish this parasite advantag ... | 2004 | 15679631 |
| host adaptor proteins gab1 and crkii promote inlb-dependent entry of listeria monocytogenes. | the bacterial surface protein inlb mediates internalization of listeria monocytogenes into mammalian cells through interaction with the host receptor tyrosine kinase, met. inlb/met interaction results in activation of the host phosphoinositide (pi) 3-kinase p85-p110, an event required for bacterial entry. p85-p110 activation coincides with tyrosine phosphorylation of the host adaptor gab1, and formation of complexes between gab1 and the p85 regulatory subunit of pi 3-kinase. when phosphorylated ... | 2005 | 15679846 |
| changes in growth, rrna content, and cell morphology of listeria monocytogenes induced by co2 up- and downshift. | cell morphology, rrna content, and growth were examined for listeria monocytogenes lo28 and egd, respectively, grown in brain-heart infusion (bhi) and on slices of sausage at 10 degrees c in 100% co2, 100% n2, and air. in co2, filamentous cells were formed by both strains on sausage slices and by l. monocytogenes egd in bhi. filamentation was not induced by anaerobiosis only. fluorescent in situ rrna hybridization (fish) of cells grown in bhi showed that the l. monocytogenes egd filaments consis ... | 2005 | 15681042 |
| enhanced resistance to gram-positive bacterium and increased susceptibility to bacterial endotoxin in mice sensitized with propionibacterium acnes: involvement of toll-like receptor. | mice sensitized with propionibacterium acnes showed an enhanced resistance against infection with listeria monocytogenes in contrast to the increased susceptibility to lps-induced endotoxin shock. the enhanced protection to l. monocytogenes was mediated by activated innate immunity but not by generation of listeria-specific acquired immunity. after infection with l. monocytogenes, the elimination of bacteria was observed earlier in accordance with a higher level of endogenous cytokine production ... | 2005 | 15681160 |
| supportive and inhibitory elements of a putative prfa-dependent promoter in listeria monocytogenes. | elements essential for prfa-dependent transcription were analysed on two promoters of listeria monocytogenes, the prfa-dependent promoter of the phospholipase gene plca (pplca) and a putative promoter of the aroa gene (paroa2) which contains a similar prfa-binding site and a similar -10 box as pplca but does not function as prfa-dependent promoter. we constructed a series of hybrid plca-aroa promoters by exchanging corresponding sequence elements of these two 'promoters'. the results showed that ... | 2005 | 15686548 |
| a prfa-regulated bile exclusion system (bile) is a novel virulence factor in listeria monocytogenes. | the ability to colonize the gall bladder has recently been shown to be an important feature of virulent listeria monocytogenes (j. hardy, k. p. francis, m. deboer, p. chu, k. gibbs, c. h. contag. science 303: 851-853, 2004). we suggest that the cytotoxic effects of bile may be increased upon release from the gall bladder into the upper small intestine, and report the identification of a novel bile exclusion system which plays an essential role in intestinal colonization and virulence of l. monoc ... | 2005 | 15686563 |
| comparative assessment of acid, alkali and salt tolerance in listeria monocytogenes virulent and avirulent strains. | listeria monocytogenes is an opportunistic bacterial pathogen of man and animals that has the capacity to survive under extreme environmental conditions. while our knowledge on l. monocytogenes and its ability to sustain within wide ph and temperature ranges and salt concentrations has been largely built on the virulent strains of this species, relatively little is known about avirulent strains in this regard. in this study, we extend our analysis on avirulent l. monocytogenes strains. by subjec ... | 2005 | 15686837 |
| [the incidence of bacterial cns infections in roe deer (capreolus capreolus), red deer (cervus elaphus) and chamois (rupicapra rupicapra) in bavaria]. | brain samples of 849 wild ruminants (654 roe deer, 189 red deer and 6 chamois) from bavaria were examined for the occurrence of encephalopathies caused by bacteria, using cultural, serological and genetic methods. in addition, 87 brain samples were investigated histologically for clarification of the pathogenetic relevance of specific microorganisms. using conventional bacteriological methods, 464 different bacteria were isolated. 229 of them could be differentiated to the genus level and 235 to ... | 2005 | 15690635 |
| effect of salting and cold-smoking process on the culturability, viability, and virulence of listeria monocytogenes strain scott a. | the aim of the present study was to determine the effect of the different steps of the cold-smoking process and vacuum storage on the culturability and viability of listeria monocytogenes strain scott a inoculated in sterile salmon samples. additionally, the virulence of l. monocytogenes cells was assessed by intravenous inoculation of immunocompetent mice. salmon (salmo salar) portions were inoculated with l. monocytogenes at a level of 6 log cfu/g and were then dry salted (5.9%), smoked (0.74 ... | 2005 | 15690807 |
| formation of biofilms by listeria monocytogenes under various growth conditions. | eight strains of listeria monocytogenes (7644, 19112, 15313, scott a, lcdc, 10403s, slcc, and 1370) produce biofilms when grown on polyvinyl chloride microtiter well plates. the growth medium (tryptic soy broth [tsb] or modified welshimer's broth [mwb] at 32 degrees c) influenced the amount of biofilm formed; maximum biofilms were formed in mwb by six strains and in tsb by the remaining two strains. this result suggests that the growth medium is critical in development of l. monocytogenes biofil ... | 2005 | 15690808 |
| production, purification, and characterization of micrococcin go5, a bacteriocin produced by micrococcus sp. go5 isolated from kimchi. | strain go5, a bacteriocin-producing bacterium, was isolated from green onion kimchi and identified as micrococcus sp. the bacteriocin, micrococcin go5, displayed a broad spectrum of inhibitory activity against a variety of pathogenic and nonpathogenic microorganisms, as tested by the spot-on-lawn method; its activity spectrum was almost identical to that of nisin. micrococcin go5 was inactivated by trypsin (whereas nisin was not) and was completely stable at 100 degrees c for 30 min and in the p ... | 2005 | 15690819 |
| elimination of listeria monocytogenes from ready-to-eat turkey and cheese tortilla wraps using ionizing radiation. | listeria monocytogenes is a common postprocess contaminant on ready-to-eat foods including premade ready-to-eat sandwiches. one popular type of sandwich product is the tortilla wrap, which contains sliced luncheon meats and cheeses rolled within a flour tortilla. this study determined the radiation resistance of l. monocytogenes surface inoculated onto two types of commercially available wheat flour tortillas, processed cheese slices, and deli turkey meat. the d10-values for l. monocytogenes (th ... | 2005 | 15690820 |
| effect of prior growth conditions on the thermal inactivation of 13 strains of listeria monocytogenes in two heating menstrua. | the thermal tolerance of 13 listeria monocytogenes strains was tested using a submerged heating coil apparatus. the strains were grown individually for 18 h at 37 degrees c in acidogenic tryptic soy broth (without dextrose) supplemented with 1% glucose and 1% glutamine (tsb+g) or nonacidogenic tryptic soy broth supplemented with 1% glutamine but containing no glucose (dextrose) (tsb-g). the former medium results in cells induced for ph-dependent, stationary-phase acid resistance, whereas the lat ... | 2005 | 15690821 |
| inhibition of listeria monocytogenes and salmonella by natural antimicrobials and high hydrostatic pressure in sliced cooked ham. | the effectiveness of nisin, lactate salts, and high hydrostatic pressure to inhibit the growth of listeria monocytogenes and salmonella in sliced cooked ham was studied through a combination of pcr-based detection methods, most probable number, and classical microbial enumeration techniques (international organization for standardization protocols). a synergistic effect to inhibit a cocktail of listeria monocytogenes ctc1010, ctc1011, and ctc1034 was observed between potassium lactate, high hydr ... | 2005 | 15690822 |
| low prevalence of listeria monocytogenes in human stool. | listeria monocytogenes is a foodborne pathogen that is found widely in the environment and in a variety of ready-to-eat foods, yet human invasive infection is relatively rare (five cases per million people annually in the united states). despite wide exposure to this organism, little is known about the prevalence of l. monocytogenes in human stool, and it is not known whether human fecal dispersal contributes to human foodborne transmission. we cultured 827 stool specimens (well formed and loose ... | 2005 | 15690823 |
| fate of pathogens present in livestock wastes spread onto fescue plots. | fecal wastes from a variety of farmed livestock were inoculated with livestock isolates of escherichia coli o157, listeria monocytogenes, salmonella, campylobacter jejuni, and cryptosporidium parvum oocysts at levels representative of the levels found in naturally contaminated wastes. the wastes were subsequently spread onto a grass pasture, and the decline of each of the zoonotic agents was monitored over time. there were no significant differences among the decimal reduction times for the bact ... | 2005 | 15691918 |
| bias in the listeria monocytogenes enrichment procedure: lineage 2 strains outcompete lineage 1 strains in university of vermont selective enrichments. | listeria monocytogenes can be isolated from a range of food products and may cause food-borne outbreaks or sporadic cases of listeriosis. l. monocytogenes is divided into three genetic lineages and 13 serotypes. strains of three serotypes (1/2a, 1/2b, and 4b) are associated with most human cases of listeriosis. of these, strains of serotypes 1/2b and 4b belong to lineage 1, whereas strains of serotype 1/2a and many other strains isolated from foods belong to lineage 2. l. monocytogenes is isolat ... | 2005 | 15691954 |
| screening for bacillus isolates in the broiler gastrointestinal tract. | spores from a number of different bacillus species are currently being used as human and animal probiotics, although their mechanisms of action remain poorly understood. here we describe the isolation of 237 presumptive gut-associated bacillus spp. isolates that were obtained by heat and ethanol treatment of fecal material from organically reared broilers followed by aerobic plating. thirty-one representative isolates were characterized according to their morphological, physiological, and bioche ... | 2005 | 15691955 |
| nod2-dependent regulation of innate and adaptive immunity in the intestinal tract. | the gene encoding the nod2 protein is frequently mutated in crohn's disease (cd) patients, although the physiological function of nod2 in the intestine remains elusive. here we show that protective immunity mediated by nod2 recognition of bacterial muramyl dipeptide is abolished in nod2-deficient mice. these animals are susceptible to bacterial infection via the oral route but not through intravenous or peritoneal delivery. nod2 is required for the expression of a subgroup of intestinal anti-mic ... | 2005 | 15692051 |
| the role of peptidoglycan in pathogenesis. | bacterial pathogens rely on a variety of virulence factors to establish the colonization of a new niche. although peptidoglycan and its muropeptide derivatives have been known to possess potent biological properties, until recently the molecular bases were poorly understood. with the identification of the cytosolic surveillance mechanism mediated by the nucleotide-binding oligomerization domain (nod)1 and nod2 proteins, which detect unique peptidoglycan-derived muropeptides, these muropeptides s ... | 2005 | 15694856 |
| modelling the effect of ethanol on growth rate of food spoilage moulds. | the effect of ethanol (e) on the radial growth rate (mu) of food spoilage moulds (aspergillus candidus, aspergillus flavus, aspergillus niger, cladosporium cladosporioides, eurotium herbariorum, mucor circinelloides, mucor racemosus, paecilomyces variotii, penicillium chrysogenum, penicillium digitatum, rhizopus oryzae and trichoderma harzianum) was assessed in potato dextrose agar (pda) medium at a(w) 0.99, 25 degrees c. in order to model this effect, the monod type equation described previousl ... | 2005 | 15698687 |
| precursor and temperature modulation of fatty acid composition and growth of listeria monocytogenes cold-sensitive mutants with transposon-interrupted branched-chain alpha-keto acid dehydrogenase. | branched-chain fatty acids (bcfas) typically constitute more than 90 % of the fatty acids of listeria monocytogenes. the authors have previously described two tn917-induced, cold-sensitive, bcfa-deficient (<40 %) l. monocytogenes mutants (cld-1 and cld-2) with lowered membrane fluidity. sequence analyses revealed that tn917 was inserted into different genes of the branched-chain alpha-keto acid dehydrogenase cluster (bkd) in these two mutants. the cold-sensitivity and bcfa deficiency of cld-1, i ... | 2005 | 15699210 |
| foodborne enteric infections. | foodborne infections are estimated to affect one in four americans each year. most these (67%) are caused by the norwalk-like viruses, but campylobacter and nontyphoidal salmonellae together account for about one fourth of cases of illness in which a pathogen can be detected. less common bacterial infections, such as with listeria monocytogenes and the shiga toxin-producing escherichia coli, cause fewer infections but are important because of their severe complications or high mortality rate, or ... | 2003 | 15699888 |
| [cerebral abscess in an immunosuppressed patient]. | 2005 | 15701332 | |
| neurolisteriosis causing hydrocephalus, trapped fourth ventricle, hindbrain herniation and syringomyelia. | central nervous system infection by listeria monocytogenes is relatively uncommon, but is known to be highly morbid and fatal. we describe a case of listeria meningoencephalitis, wherein the acute episode was followed by a hitherto unreported conglomeration of severe and progressive neurological sequelae, in the form of supratentorial hydrocephalus, aqueduct block, trapped fourth ventricle, hindbrain herniation and syringomyelia. pertinent literature is reviewed and the pathogenesis of the obser ... | 2004 | 15702836 |
| inhibition of rab5a exchange activity is a key step for listeria monocytogenes survival. | listeria monocytogenes (lm) modifies the phagocytic compartment by targeting rab5a function through an unknown mechanism. inhibition of rab5a exchange by lm can be considered the main virulence mechanism as it favours viability of the parasite within the phagosome as well as the exclusion of putative listericidal lysosomal proteases such as cathepsin-d. the significance of this survival mechanism is evidenced by the overexpression of rab5a mutants in cho cells that promoted gdp exchange on rab5a ... | 2005 | 15702993 |
| molecular and experimental virulence of listeria monocytogenes strains isolated from cases with invasive listeriosis and febrile gastroenteritis. | we analyzed 27 listeria monocytogenes strains of serotypes 1/2b and 4b, from invasive and gastroenteric listeriosis, for molecular and experimental virulence. molecular virulence was tested by pcr for the presence of 8 major virulence-associated genes and genetic polymorphisms through restriction enzyme analysis; genomic dna typing using pulsed-field gel electrophoresis was also performed. experimental virulence was evaluated through intra-peritoneal and intra-gastric mouse virulence assays. our ... | 2005 | 15708319 |
| dna prime listeria boost induces a cellular immune response to siv antigens in the rhesus macaque model that is capable of limited suppression of siv239 viral replication. | dna vaccines and recombinant listeria monocytogenes that express and secrete siv gag and env antigens were combined in a nonhuman primate prime-boost immunogenicity study followed by a challenge with siv239. we report that recombinant dna vaccine delivered intramuscularly, and recombinant l. monocytogenes delivered orally each individually have the ability to induce cd8+ and cd4+ t cell immune responses in a nonhuman primate. four rhesus monkeys were immunized at weeks 0, 4, 8, and 12 with the p ... | 2005 | 15708595 |
| evolutionary history of the genus listeria and its virulence genes. | the genus listeria contains the two pathogenic species listeria monocytogenes and listeria ivanovii and the four apparently apathogenic species listeria innocua, listeria seeligeri, listeria welshimeri, and listeria grayi. pathogenicity of the former two species is enabled by an approximately 9 kb virulence gene cluster which is also present in a modified form in l. seeligeri. for all listeria species, the sequence of the virulence gene cluster locus and its flanking regions was either determine ... | 2005 | 15709360 |
| listeria monocytogenes in the chinese food system: strain characterization through partial acta sequencing and tissue-culture pathogenicity assays. | human listeriosis is generally caused by consumption of ready-to-eat (rte) foods that are stored for extended periods of time at refrigeration temperatures and that permit the growth of the causative agent, listeria monocytogenes. food-consumption patterns in china are undergoing rapid changes and more regular consumption of refrigerated-storage rte foods may increase the risk of human listeriosis. in total, 40 l. monocytogenes isolates were obtained from food (n=32) and sewage (n=6) samples and ... | 2005 | 15713604 |
| outbreak of listeriosis among mexican immigrants as a result of consumption of illicitly produced mexican-style cheese. | in 2000, an outbreak of listeriosis among hispanic persons was identified in winston-salem, north carolina. the objectives of the present study were to identify the source of, strains associated with, and risk factors for listeria monocytogenes infection for patients affected by the outbreak. | 2005 | 15714412 |
| the role of tcr stimulation and tgf-beta in controlling the expression of cd94/nkg2a receptors on cd8 t cells. | following antigen recognition, murine cd8 t cells express cd94/nkg2a receptors. our results show that this up-regulation occurs rapidly in vitro and is accompanied by an approximately 8-fold increase in cd94 and approximately 125-fold increase in nkg2a mrna. in contrast, only a twofold increase in nkg2c mrna is noted. the addition of tgf-beta, but not il-10, il-12 or il-15, leads to a further increase in cell membrane expression of these receptors, as well as a approximately 6-fold increase in m ... | 2005 | 15714583 |
| carrier status for listeria monocytogenes and other listeria species in free range farm and market healthy indigenous chickens and ducks. | listeria organisms are documented to be zoonotic; one of the sources of infection is the domestic fowl where it could occur as in apparent infection. the carriage of listeria monocytogenes and other listeria in indigenous birds has not been documented in kenya. | 2004 | 15715131 |
| sensitivity of listeria species, recovered from indigenous chickens to antibiotics and disinfectants. | resistance of bacteria to antibiotics and disinfectants has been reported widely in the world. listeria monocytogenes is no exception, although normally it tends to be variably sensitive to many antibiotics and disinfectants. | 2004 | 15715132 |