Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| screening of mercury-resistant and indole-3-acetic acid producing bacterial-consortium for growth promotion of cicer arietinum l. | mercury resistant (hg(r) ) bacteria were screened from industrial effluents and effluents-polluted rhizosphere soils near to districts kasur and sheikhupura, pakistan. out of 60 isolates, three bacterial strains, bacillus sp. az-1, bacillus cereus az-2, and enterobacter cloacae az-3 showed hg-resistance as 20 μg ml(-1) of hgcl2 and indole-3-acetic acid (iaa) production as 8-38 μg ml(-1) . biochemical and molecular characterization of selected bacteria was confirmed by 16s ribotyping. mercury res ... | 2017 | 27911010 |
| development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of shigella spp. | shigella spp., the etiological agent of shigellosis or "bacillary dysentery," are responsible for considerable morbidity and mortality in excess of a million deaths globally per year. although pcr-based techniques (such as pcr-based dipstick biosensors) have been used for the molecular diagnosis of infectious disease, these assays were restricted due to the need for a sophisticated thermal cycling apparatus to denature target templates. to facilitate simple and rapid detection of target pathogen ... | 2016 | 27917160 |
| clonal spread of mcr-1 in pmqr-carrying st34 salmonella isolates from animals in china. | since initial identification in china, the widespread geographical occurrence of plasmid-mediated colistin resistance gene mcr-1 in enterobacteriaceae has been of great concern. in this study, a total of 22 salmonella enterica were resistant to colistin, while only five isolates which belonged to st34 salmonella enterica serovar typhimurium (s. typhimurium) were mcr-1 positive. four of them shared nearly identical pfge type, although they were from different host species and diverse geographical ... | 2016 | 27917926 |
| a prolonged outbreak of kpc-3-producing enterobacter cloacae and klebsiella pneumoniae driven by multiple mechanisms of resistance transmission at a large academic burn center. | klebsiella pneumoniae carbapenemase (kpc)-producing enterobacter cloacae has been recently recognized in the united states. whole-genome sequencing (wgs) has become a useful tool for analysis of outbreaks and for determining transmission networks of multidrug-resistant organisms in health care settings, including carbapenem-resistant enterobacteriaceae (cre). we experienced a prolonged outbreak of cre e. cloacae and k. pneumoniae over a 3-year period at a large academic burn center despite rigor ... | 2017 | 27919898 |
| salmonella typhimurium and multidirectional communication in the gut. | the mammalian digestive tract is home to trillions of microbes, including bacteria, archaea, protozoa, fungi, and viruses. in monogastric mammals the stomach and small intestine harbor diverse bacterial populations but are typically less populated than the colon. the gut bacterial community (microbiota hereafter) varies widely among different host species and individuals within a species. it is influenced by season of the year, age of the host, stress and disease. ideally, the host and microbiot ... | 2016 | 27920756 |
| comparison of gram-negative and gram-positive hematogenous pyogenic spondylodiscitis: clinical characteristics and outcomes of treatment. | to the best of our knowledge, no study has compared gram-negative bacillary hematogenous pyogenic spondylodiscitis (gnb-hps) with gram-positive coccal hematogenous pyogenic spondylodiscitis (gpc-hps) regarding their clinical characteristics and outcomes. | 2016 | 27923346 |
| immunomodulation after ischemic stroke: potential mechanisms and implications for therapy. | brain injuries are often associated with intensive care admissions, and carry high morbidity and mortality rates. ischemic stroke is one of the most frequent causes of injury to the central nervous system. it is now increasingly clear that human stroke causes multi-organ systemic disease. brain inflammation may lead to opposing local and systemic effects. suppression of systemic immunity by the nervous system could protect the brain from additional inflammatory damage; however, it may increase t ... | 2016 | 27923376 |
| mechanisms of evolution in high-consequence drug resistance plasmids. | the dissemination of resistance among bacteria has been facilitated by the fact that resistance genes are usually located on a diverse and evolving set of transmissible plasmids. however, the mechanisms generating diversity and enabling adaptation within highly successful resistance plasmids have remained obscure, despite their profound clinical significance. to understand these mechanisms, we have performed a detailed analysis of the mobilome (the entire mobile genetic element content) of a set ... | 2016 | 27923922 |
| hospital-acquired infections after cardiac surgery and current physician practices: a retrospective cohort study. | the management of hospital-acquired infections (hais) with respect to physician practices remains largely unexplored despite increasing efforts to standardize care. in the present study, we report findings from a 2-month audit of all patients that have undergone cardiac surgery at a large referral center in atlantic canada. | 2016 | 27924169 |
| hospital-acquired infections after cardiac surgery and current physician practices: a retrospective cohort study. | the management of hospital-acquired infections (hais) with respect to physician practices remains largely unexplored despite increasing efforts to standardize care. in the present study, we report findings from a 2-month audit of all patients that have undergone cardiac surgery at a large referral center in atlantic canada. | 2016 | 27924169 |
| phenotypic and molecular detection of the bla kpc gene in clinical isolates from inpatients at hospitals in são luis, ma, brazil. | bacteria that produce klebsiella pneumoniae carbapenemases (kpcs) are resistant to broad-spectrum β-lactam antibiotics. the objective of this study was to phenotypically and genotypically characterize the antibiotic susceptibility to carbapenems of 297 isolates recovered from clinical samples obtained from inpatients at 16 hospitals in são luis (maranhão, brazil). | 2016 | 27927163 |
| emergence of cmy-2- and dha-1-type ampc β-lactamases in enterobacter cloacae isolated from several hospitals of qazvin and tehran, iran. | the emergence of plasmid-mediated ampc (pampc) β-lactamases conferring resistance to third-generation cephalosporins has become a major clinical concern worldwide. the aims of this study were to determine the prevalence of pampc-producing e. cloacae isolates and typing of them in qazvin and tehran provinces, iran. | 2016 | 27928483 |
| a retrospective study of pyogenic liver abscess focusing on klebsiella pneumoniae as a primary pathogen in china from 1994 to 2015. | pyogenic liver abscess (pla) is a common intra-abdominal infection in adults. in this study, we aim to explore demographic and clinical characteristics of pla focusing on klebsiella pneumoniae (k. pneumoniae) induced pla (kp-pla) in mainland china. a retrospective review of medical records from all patients with kp-pla admitted to a tertiary teaching hospital over a 21-year period (1994-2015) was performed. among 296 pla cases with confirmed culture-positive data, k. pneumoniae was revealed as t ... | 2016 | 27929082 |
| strain-level discrimination of shiga toxin-producing escherichia coli in spinach using metagenomic sequencing. | consumption of fresh bagged spinach contaminated with shiga toxin-producing escherichia coli (stec) has led to severe illness and death; however current culture-based methods to detect foodborne stec are time consuming. since not all stec strains are considered pathogenic to humans, it is crucial to incorporate virulence characterization of stec in the detection method. in this study, we assess the comprehensiveness of utilizing a shotgun metagenomics approach for detection and strain-level iden ... | 2016 | 27930729 |
| searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds. | the aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and gram-positive bacterial strains, including methicillin-resistant staphylococcus aureus (mrsa) and its biofilm, to probe for potential lead structures. | 2017 | 27930849 |
| production, characterization, and application of bacillus licheniformis w16 biosurfactant in enhancing oil recovery. | the biosurfactant production by bacillus licheniformis w16 and evaluation of biosurfactant based enhanced oil recovery (eor) using core-flood under reservoir conditions were investigated. previously reported nine different production media were screened for biosurfactant production, and two were further optimized with different carbon sources (glucose, sucrose, starch, cane molasses, or date molasses), as well as the strain was screened for biosurfactant production during the growth in different ... | 2016 | 27933041 |
| gram positive and gram negative bacteria differ in their sensitivity to cold plasma. | cold atmospheric-pressure plasma (cap) is a relatively new method being investigated for antimicrobial activity. however, the exact mode of action is still being explored. here we report that cap efficacy is directly correlated to bacterial cell wall thickness in several species. biofilms of gram positive bacillus subtilis, possessing a 55.4 nm cell wall, showed the highest resistance to cap, with less than one log10 reduction after 10 min treatment. in contrast, biofilms of gram negative pseudo ... | 2016 | 27934958 |
| intense pre-admission carriage and further acquisition of esbl-producing enterobacteriaceae among patients and their caregivers in a tertiary hospital in rwanda. | to assess the presence and risk factors of intestinal carriage of extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-pe) among patients admitted to the university teaching hospital of butare and among their attending caregivers, and to analyse the acquisition of esbl-pe carriage during hospital stay and associated factors. | 2017 | 27935649 |
| high prevalence of faecal carriage of esbl-producing enterobacteriaceae among children in dar es salaam, tanzania. | faecal carriage of esbl-producing bacteria is a potential risk for transmission and infection. little is known about faecal carriage of antibiotic resistance in tanzania. this study aimed to investigate the prevalence of faecal carriage of esbl-producing enterobacteriaceae and to identify risk factors for carriage among young children in tanzania. | 2016 | 27936054 |
| isolation, antimicrobial susceptibility profile and detection of sul1, blatem, and blashv in amoxicillin-clavulanate-resistant bacteria isolated from retail sausages in kampar, malaysia. | due to the overuse of antibiotics in livestock as a growth-promoting agent, the emergence of multi-antibiotic resistant bacteria is becoming a concern. | 2016 | 27942365 |
| metabolic engineering of serratia marcescens mg1 for enhanced production of (3r)-acetoin. | optically pure acetoin (ac) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites. | 2016 | 27942437 |
| inventory of extended-spectrum-β-lactamase-producing enterobacteriaceae in france as assessed by a multicenter study. | the objective of this study was to perform an inventory of the extended-spectrum-β-lactamase (esbl)-producing enterobacteriaceae isolates responsible for infections in french hospitals and to assess the mechanisms associated with esbl diffusion. a total of 200 nonredundant esbl-producing enterobacteriaceae strains isolated from clinical samples were collected during a multicenter study performed in 18 representative french hospitals. antibiotic resistance genes were identified by pcr and sequenc ... | 2017 | 27956424 |
| pediatric infectious endophthalmitis: a 271-case retrospective study at a single center in china. | pediatric infectious endophthalmitis is a serious sight-threatening disease for children. the purpose of this study was to investigate the etiology, microbiological spectrum, and visual outcomes of infectious endophthalmitis in children at a single institution in china. | 2016 | 27958225 |
| molecular and in silico analysis of a new plasmid-mediated ampc β-lactamase (cmh-2) in clinical isolates of klebsiella pneumoniae. | two klebsiella strains isolated from urine samples were positive for blaampc by pcr and showed sequence similarity with cmh-1 (98.6%) after sequencing. it also shares 82% similarity with act-1, 85% with mir-1 and 81% with the chromosomal ampc gene of enterobacter cloacae. this gene was associated with the plasmid of inck type. it has an open reading frame of 381 amino acid with four amino acid substitutions at position d144a, c189r, q192e, and a195t as compared to cmh-1. when expressed in e.coli ... | 2017 | 27964935 |
| risk factors for bacteremia in patients with urinary catheter-associated bacteriuria. | catheter-associated bacteriuria is complicated by secondary bacteremia in 0.4% to 4.0% of cases. the directly attributable mortality rate is 12.7%. | 2016 | 27965229 |
| comprehensive genome analysis of carbapenemase-producing enterobacter spp.: new insights into phylogeny, population structure, and resistance mechanisms. | knowledge regarding the genomic structure of enterobacter spp., the second most prevalent carbapenemase-producing enterobacteriaceae, remains limited. here we sequenced 97 clinical enterobacter species isolates that were both carbapenem susceptible and resistant from various geographic regions to decipher the molecular origins of carbapenem resistance and to understand the changing phylogeny of these emerging and drug-resistant pathogens. of the carbapenem-resistant isolates, 30 possessed blakpc ... | 2016 | 27965456 |
| high prevalence of gut microbiota colonization with broad-spectrum cephalosporin resistant enterobacteriaceae in a tunisian intensive care unit. | healthcare-associated infections due to cefotaxime-resistant (ctx-r) enterobacteriaceae have become a major public health threat, especially in intensive care units (icus). often acquired nosocomially, ctx-r enterobacteriaceae can be introduced initially by patients at admission. this study aimed to determine the prevalence and genetic characteristics of ctx-r enterobacteriaceae-intestinal carriage in icu patients, to evaluate the rate of acquisition of these organisms during hospitalization, an ... | 2016 | 27965626 |
| evaluation of machine learning and rules-based approaches for predicting antimicrobial resistance profiles in gram-negative bacilli from whole genome sequence data. | the time-to-result for culture-based microorganism recovery and phenotypic antimicrobial susceptibility testing necessitates initial use of empiric (frequently broad-spectrum) antimicrobial therapy. if the empiric therapy is not optimal, this can lead to adverse patient outcomes and contribute to increasing antibiotic resistance in pathogens. new, more rapid technologies are emerging to meet this need. many of these are based on identifying resistance genes, rather than directly assaying resista ... | 2016 | 27965630 |
| microbial ecology: where are we now? | conventional microbiological methods have been readily taken over by newer molecular techniques due to the ease of use, reproducibility, sensitivity and speed of working with nucleic acids. these tools allow high throughput analysis of complex and diverse microbial communities, such as those in soil, freshwater, saltwater, or the microbiota living in collaboration with a host organism (plant, mouse, human, etc). for instance, these methods have been robustly used for characterizing the plant (rh ... | 2016 | 27975077 |
| reduction of quinones and nitroaromatic compounds by escherichia coli nitroreductase a (nfsa): characterization of kinetics and substrate specificity. | nfsa, a major fmn-associated nitroreductase of e. coli, reduces nitroaromatic compounds via consecutive two-electron transfers. nfsa has potential applications in the biodegradation of nitroaromatic environment pollutants, e.g. explosives, and is also of interest for the anticancer strategy gene-directed enzyme prodrug therapy. however, the catalytic mechanism of nfsa is poorly characterized. here we examined the nadph-dependent reduction of quinones (n = 16) and nitroaromatic compounds (n = 12) ... | 2017 | 27986535 |
| structural insight into mode of binding of meropenem to ctx-m-15 type β-lactamase. | among enterobacteriaceae, ctx-m type extended spectrum beta lactamase confers potent hydrolytic activity against cephalosporin group of antibiotics. strains producing ctx-m type beta lactamase enzymes, show high level of resistance against cefotaxime. therefore carbapenem antibiotics are used against beta lactamase producing strains. hence, this study was designed to understand an insight of molecular basis of ctx-m-15 interaction with meropenem, and its effect on ctx-m-15 efficiency. clinical s ... | 2017 | 27986632 |
| predominance of ctx-m-15 among esbl producers from environment and fish gut from the shores of lake victoria in mwanza, tanzania. | extended-spectrum beta-lactamase (esbl)-producing bacteria are a common cause of healthcare and community-associated infections worldwide. the distribution of such isolates in the environment and their presence in fish as a result of sewage contamination is not well-studied. here we examined fish and environmental samples from mwanza city for the presence of esbl-producing bacteria. from 196 fish sampled from local markets, 26 (13.3%) contained lactose-fermenting esbl-producing bacteria, while 3 ... | 2016 | 27990135 |
| evaluation of the xpert carba-r (cepheid) assay using contrived bronchial specimens from patients with suspicion of ventilator-associated pneumonia for the detection of prevalent carbapenemases. | there is a critical need for rapid diagnostic methods for multidrug-resistant (mdr) pathogens in patients with a suspicion of ventilator-associated pneumonia (vap). the xpert carba-r detects 5 targets for carbapenemase-producing organisms (blakpc, blandm, blavim, blaoxa-48, and blaimp-1). our objective was to evaluate the performance of this assay directly on bronchial aspirates and to correlate the cycle number for a positive result (ct) with the bacterial count. bronchial aspirates from patien ... | 2016 | 27992521 |
| corrigendum to "carbapenem susceptibility among escherichia coli, klebsiella pneumoniae, and enterobacter cloacae isolates obtained from patients in intensive care units in taiwan in 2005, 2007, and 2009" [diagn microbiol infect dis 2015;81(4):290-295]. | 2017 | 27993423 | |
| the most common detected bacteria in sputum of patients with community acquired pneumonia (cap) treated in hospital. | community acquired pneumonia (cap) is the most common infective pulmonary disease. | 2016 | 27994296 |
| intravenous colistin use for multidrug-resistant gram-negative infections in pediatric patients. | the emergence of infections due to multidrug-resistant gram-negative bacilli (mdr-gnb) has led to the resurrection of colistin use. the data on colistin use and drug-related adverse effects in children are scarce. | 2016 | 27994915 |
| recurrent episodes of candidemia due to candida glabrata, candida tropicalis and candida albicans with acquired echinocandin resistance. | mixed fungal infection and acquired echinocandin resistance of candida spp. remain infrequent. in this study we have reported the case of a patient hospitalized for tuberculosis who experienced multiple infections due to three common candida species (c. albicans, c. glabrata, c. tropicalis). furthermore, consecutive isolates from blood cultures and heart valve were found resistant to azoles (c. tropicalis) and to echinocandin with either novel (c. tropicalis) or previously described (c. albicans ... | 2016 | 27995055 |
| longipin: an amyloid antimicrobial peptide from the harvestman acutisoma longipes (arachnida: opiliones) with preferential affinity for anionic vesicles. | in contrast to vertebrate immune systems, invertebrates lack an adaptive response and rely solely on innate immunity in which antimicrobial peptides (amps) play an essential role. most of them are membrane active molecules that are typically unstructured in solution and adopt secondary/tertiary structures upon binding to phospholipid bilayers. this work presents the first characterization of a constitutive amp from the hemolymph of an opiliones order animal: the harvestman acutisoma longipes. th ... | 2016 | 27997568 |
| evaluation of a commercial multiplex pcr assay for detection of pathogen dna in blood from patients with suspected sepsis. | the magicplex sepsis real-time test (mst) is a commercial multiplex pcr that can detect more than 90 different pathogens in blood, with an analysis time of six hours. the aim of the present study was to evaluate this method for the detection of bloodstream infection (bsi). an edta whole blood sample for mst was collected together with blood cultures (bc) from patients with suspected sepsis at the emergency department of a university hospital. among 696 study patients, 322 (46%) patients were pos ... | 2016 | 27997618 |
| development and application of a synthetically-derived lead biosensor construct for use in gram-negative bacteria. | the use of lead in manufacturing has decreased significantly over the last few decades. however, previous widespread use of lead-containing products and their incorrect disposal has resulted in environmental contamination. accumulation of harmful quantities of lead pose a threat to all living organisms, through inhalation, ingestion, or direct contact, resulting in lead poisoning. this study utilized synthetic biology principles to develop plasmid-based whole-cell bacterial biosensors for detect ... | 2016 | 27999352 |
| purification, characterization and comparison between two new l-asparaginases from bacillus pg03 and bacillus pg04. | l-asparaginase has been used as a chemotherapeutic agent in treatment of lymphoblastic leukemia. in the present investigation, bacillus sp. pg03 and bacillus sp. pg04 were studied. | 2016 | 27999622 |
| neonatal bacterial conjunctivitis in korea in the 21st century. | to investigate the microbial cause and antibiotic susceptibility of neonatal bacterial conjunctivitis in korean patients in the 21st century. | 2017 | 28002109 |
| salvage plasmapheresis for post-hepatectomy liver failure. | in the latest decades, an important change has been registered in liver surgery related to the progress of surgical techniques, critical care, and postoperative treatment, allowing a sharp decrease in mortality and morbidity. however, management of post-hepatectomy liver failure (phlf) still remains a challenge and no supportive treatment has been found to be generally effective. the present study is a reappraisal of plasmapheresis as a potential supportive measure in patients with phlf followin ... | 2016 | 28003948 |
| combination therapy with vancomycin-loaded calcium sulfate and vancomycin-loaded pmma in the treatment of chronic osteomyelitis. | chronic post-traumatic and postoperative osteomyelitis is a refractory disease which results in significant morbidity and mortality. the effect of combination therapy with vancomycin-loaded calcium sulfate and vancomycin-loaded polymethyl methacrylate (pmma) was unknown. | 2016 | 28007027 |
| [epidemiology and risk factors of patients with intra-abdominal postsurgical infection treated with tigecycline: a cohort study]. | to study a cohort of patients with intra-abdominal postsurgical infection treated with tigecycline to analyze its effectiveness and mortality related factors. | 2017 | 28010058 |
| a mechanism of synergistic effect of streptomycin and cefotaxime on ctx-m-15 type β-lactamase producing strain of e. cloacae: a first report. | a blactx-m-15 gene is one of the most prevalent resistant marker found in member of enterobacteriaceae. it encodes cefotaxime hydrolysing β-lactamase-15 (ctx-m-15) causing resistance against beta lactam antibiotics. since single antibiotic therapy fails to control infection caused by multidrug resistance strain, therefore combination therapy was came into practice as an effective treatment. we have first time explained the mechanism where two antibiotics of different classes work against resista ... | 2016 | 28018328 |
| antibiofilm efficacy of green synthesized graphene oxide-silver nanocomposite using lagerstroemia speciosa floral extract: a comparative study on inhibition of gram-positive and gram-negative biofilms. | biofilm architecture provides bacteria with enhanced antibiotic resistance, thus raising the need to search for alternative therapies that can inhibit the bacterial colonization. in the present study, we synthesized graphene oxide-silver nanocomposite (go-ag) by non-toxic and eco-friendly route using a floral extract of legistromia speciosa (l.) pers. the gas chromatography-mass spectrometry (gc-ms) analysis of plant extract revealed the presence of compounds which can simultaneously act as redu ... | 2017 | 28027944 |
| management of acute hematogenous infection following total knee arthroplasty: a case series of 11 patients. | acute hematogenous infection is a devastating complication that can occur after total knee arthroplasty (tka). the best strategies for management of this infection remain controversial. two-stage revision has been well described as the gold standard for the management of chronic late infections. however, there is a paucity of information presently available on the management and outcomes of patients treated for acute hematogenous infections. the purpose of the present study was to report the out ... | 2016 | 28032700 |
| draft genome sequences of biosafety level 2 opportunistic pathogens isolated from the environmental surfaces of the international space station. | the draft genome sequences of 20 biosafety level 2 (bsl-2) opportunistic pathogens isolated from the environmental surfaces of the international space station (iss) were presented. these genomic sequences will help in understanding the influence of microgravity on the pathogenicity and virulence of these strains when compared with earth strains. | 2016 | 28034853 |
| the clinical impact of rapid, direct maldi-tof identification of bacteria from positive blood cultures. | faster identification of bacterial isolates from blood cultures can enable earlier clinical intervention for patients with sepsis. we evaluated the clinical impact of direct identification of micro-organisms from positive blood cultures using maldi-tof. | 2016 | 28036369 |
| the pharmacodynamics of avibactam in combination with ceftaroline or ceftazidime against β-lactamase-producing enterobacteriaceae studied in an in vitro model of infection. | pharmacodynamics of β-lactamase inhibitors are an area of intense interest as new β-lactam/β-lactamase inhibitor combinations enter clinical development and clinical practice. avibactam, a non-β-lactam β-lactamase inhibitor, has been combined with ceftaroline or ceftazidime but these two combinations have not been directly compared. | 2017 | 28039276 |
| abstracts. | 2017 | 28044356 | |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| prevalence of multidrug-resistant organisms in refugee patients, medical tourists and domestic patients admitted to a german university hospital. | patients with contact to healthcare-system in high-prevalence countries (hpc) and refugee patients in hospital settings (ref) have previously been identified to be at risk of carrying multidrug-resistant organisms (mdro). comparative studies addressing the epidemiology of mdro in patients transferred from hospitals abroad (abroad) and ref are lacking but are necessary to introduce refined infection control measures. | 2017 | 28056820 |
| risk factors and characteristics of blood stream infections in patients with newly diagnosed multiple myeloma. | patients with multiple myeloma are generally immune-compromised either due to pronounced depression in primary antibody responses or because of anti-myeloma therapy. infection is a major risk factor for early deaths among these patients. the impact of blood stream infections (bsi) on newly diagnosed myeloma patients has been less studied. we aimed to study the incidence and risk factors of bsi within 3 months after diagnosis of multiple myeloma in a tertiary referral center. | 2017 | 28056867 |
| the efficacy of noble metal alloy urinary catheters in reducing catheter-associated urinary tract infection. | catheter-associated urinary tract infection (cauti) is the most common device-related healthcare-acquired infection. cauti can be severe and lead to bacteremia, significant morbidity, prolonged hospital stay, and high antibiotic consumption. | 2016 | 28057985 |
| antibacterial activities of the methanol extract, fractions and compounds from elaeophorbia drupifera (thonn.) stapf. (euphorbiaceae). | elaeophorbia drupifera (thonn.) stapf. (euphorbiaceae) is used in cameroonian folk medicine to treat several ailments including bacterial-related diseases such as skin infections. in this study, the methanol extract from the leaves (edl), fractions (edla-d), sub-fractions edlc1-7 and edlc31-35 as well as isolated compounds were tested for their antimicrobial activities against a panel of gram-negative and gram-positive bacteria including multidrug resistant (mdr) phenotypes. | 2017 | 28061888 |
| multiple cross displacement amplification combined with gold nanoparticle-based lateral flow biosensor for detection of vibrio parahaemolyticus. | vibrio parahaemolyticus (v. parahaemolyticus) is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. in the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (mcda-lfb) to detect the toxr gene of v. parahaemolyticus in dna extracts from pure cultures and spiked oyster homogenates. amplification was carried out at a constant temperature (62°c) for only 30 min, and amplification products were directly applied to ... | 2016 | 28066368 |
| altered outer membrane transcriptome balance with ampc overexpression in carbapenem-resistant enterobacter cloacae. | the growing incidence of multidrug-resistant (mdr) bacteria is an emerging challenge in modern medicine. the utility of carbapenems, considered "last-line" agents in therapy of infections caused by mdr pathogens, is being diminished by the growing incidence of various resistance mechanisms. enterobacter cloacae have lately begun to emerge as an important pathogen prone to exhibiting multiple drug resistance. we aimed to investigate the molecular basis of carbapenem-resistance in 44 e. cloacae cl ... | 2016 | 28066375 |
| shifted focus of bronchoalveolar lavage in patients with suspected thoracic malignancy: an analysis of 224 patients. | bronchoscopies are extensively adopted for diagnosing and staging thoracic malignancies, but studies are missing as how to keep the process streamlined and more efficient. to evaluate current role of bronchoalveolar lavage (bal) for cancer and possible infection diagnosis when practicing comprehensive bronchoscopy for patients suspected with thoracic malignancy, and provide foundation for possible practice modification. | 2016 | 28066604 |
| intestinal enterobacteriaceae that protect nematodes from the effects of benzimidazoles. | the objective of this study was to investigate an interaction between nematodes and gut enterobacteriaceae that use benzimidazoles as a carbon source. by addressing this objective, we identified an anthelmintic resistance-like mechanism for gastrointestinal nematodes. we isolated 30 gut bacteria (family enterobacteriaceae) that subsist on and putatively catabolize benzimidazole-class anthelmintics. c. elegans was protected from the effects of benzimidazoles when co-incubated with these enterobac ... | 2016 | 28066686 |
| development of a recombinase polymerase amplification assay for detection of epidemic human noroviruses. | human norovirus is a leading cause of viral gastroenteritis worldwide. rapid detection could facilitate control, however widespread point-of-care testing is infrequently done due to the lack of robust and portable methods. recombinase polymerase amplification (rpa) is a novel isothermal method which rapidly amplifies and detects nucleic acids using a simple device in near real-time. an rt-rpa assay targeting a recent epidemic human norovirus strain (gii.4 new orleans) was developed and evaluated ... | 2017 | 28067278 |
| antimicrobial activity of ceftazidime-avibactam and comparator agents when tested against bacterial isolates causing infection in cancer patients (2013-2014). | we evaluated the antimicrobial susceptibility of 623 gram-negative organisms causing infection in patients with cancer in 52 united states hospitals (2013-2014) as part of the international network for optimal resistance monitoring (inform) program. isolates were tested for susceptibility by broth microdilution method. β-lactamase encoding genes were evaluated for all escherichia coli and klebsiella spp. with an extended-spectrum β-lactamase (esbl) phenotype by microarray-based assay. esbl-pheno ... | 2017 | 28069328 |
| proceedings of réanimation 2017, the french intensive care society international congress. | 2017 | 28074415 | |
| proceedings of réanimation 2017, the french intensive care society international congress. | 2017 | 28074416 | |
| microrna-155 is upregulated in ascites in patients with spontaneous bacterial peritonitis. | mircorna's (mir) have been recognised as important modulators of gene expression and potential biomarkers. however, they have been rarely investigated in bio fluids apart from blood. we investigated the association of mir-125b and mir-155 with complications of cirrhosis. ascites was prospectively collected from patients with cirrhosis undergoing paracentesis at our department. mir's were determined in the supernatant using qpcr and normalized by sv-40. clinical parameters were assessed at parace ... | 2017 | 28074870 |
| the influence of antibiotic prophylaxis on bacterial resistance in urinary tract infections in children with spina bifida. | bacterial resistance to antibiotics is an increasingly threatening consequence of antimicrobial exposure for many decades now. in urinary tract infections (utis), antibiotic prophylaxis (ap) increases bacterial resistance. we studied the resistance patterns of positive urinary cultures in spina bifida children on clean intermittent catheterization, both continuing and stopping ap. | 2017 | 28081719 |
| high-quality genome sequence of human pathogen enterobacter asburiae type strain 1497-78(t). | enterobacter asburiae belongs to the enterobacter cloacae complex (ecc), which comprises six heterogenic species. these bacteria can cause nosocomial infections. moreover, they are well known for antibiotic resistance features based on overproduction of ampc β-lactamases. although ecc have clinical importance, little is known about their virulence-associated properties, and very few strains from the six species have been sequenced. in this study, the type strain of e. asburiae 1497-78(t) (atcc 3 ... | 2017 | 28082144 |
| an updated review on the phytochemistry, pharmacology, and clinical trials of salacia oblonga. | salacia oblonga (s. oblonga), a perennial herb, has been used for thousands of years in ayurvedic medicine and is closely associated with prevention, treatment, and cure of various human ailments such as obesity and diabetes. a vast and wide range of chemical compounds such as polyphenols, friedelane-type triterpenes, norfriedelane-type triterpenes, eudesmane-type sesquiterpenes including various glycosides had been isolated from this plant. this review is aimed to survey the literature covering ... | 2016 | 28082793 |
| detection of ampc β-lactamase producing bacteria isolated in neonatal sepsis. | the objective of this study was to determine the occurrence and antimicrobial profile of ampc β-lactamase producing bacteria. | 2016 | 28083055 |
| intestinal bacterial colonization in the first 2 weeks of life of nigerian neonates using standard culture methods. | the pattern and timing of development of intestinal microflora in nigerian infants have been scarcely researched. this study was carried out to investigate the bacteria flora in the rectum of healthy neonates in ibadan, nigeria. | 2016 | 28083526 |
| in vitro activity of ceftazidime/avibactam against urinary isolates from patients in a phase 3 clinical trial programme for the treatment of complicated urinary tract infections. | to evaluate the in vitro activity of ceftazidime/avibactam relative to comparator agents against gram-negative isolates from a phase 3 clinical trial programme for complicated urinary tract infections (recapture). | 2017 | 28088768 |
| antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. | a wide range of medicinal and aromatic plants (maps) have been explored for their essential oils in the past few decades. essential oils are complex volatile compounds, synthesized naturally in different plant parts during the process of secondary metabolism. essential oils have great potential in the field of biomedicine as they effectively destroy several bacterial, fungal, and viral pathogens. the presence of different types of aldehydes, phenolics, terpenes, and other antimicrobial compounds ... | 2016 | 28090211 |
| bacterial plasmid-mediated quinolone resistance genes in aquatic environments in china. | emerging antimicrobial resistance is a major threat to human's health in the 21(st) century. understanding and combating this issue requires a full and unbiased assessment of the current status on the prevalence of antimicrobial resistance genes and their correlation with each other and bacterial groups. in aquatic environments that are known reservoirs for antimicrobial resistance genes, we were able to reach this goal on plasmid-mediated quinolone resistance (pmqr) genes that lead to resistanc ... | 2017 | 28094345 |
| development of an algorithm for phenotypic screening of carbapenemase-producing enterobacteriaceae in the routine laboratory. | carbapenemase-producing enterobacteriaceae (cpe) are difficult to identify among carbapenem non-susceptible enterobacteriaceae (nse). we designed phenotypic strategies giving priority to high sensitivity for screening putative cpe before further testing. | 2017 | 28095794 |
| multiple liver abscesses and bacteremia caused by streptococcus constellatus infection: a case report. | the streptococcus milleri group (smg) comprises commensal mucosal bacteria. pyogenic liver abscesses due to hematogenous smg infection are rare but can be observed even in healthy patients. in such cases, physicians should consider the existence of primary lesions that allow penetration of the smg. | 2016 | 28096994 |
| multiple liver abscesses and bacteremia caused by streptococcus constellatus infection: a case report. | the streptococcus milleri group (smg) comprises commensal mucosal bacteria. pyogenic liver abscesses due to hematogenous smg infection are rare but can be observed even in healthy patients. in such cases, physicians should consider the existence of primary lesions that allow penetration of the smg. | 2016 | 28096994 |
| emergence of blandm-7-producing enterobacteriaceae in gabon, 2016. | reports of carbapenemase-producing enterobacteriaceae in africa remain rare and assess mostly blaoxa-48-producing isolates from mediterranean countries and south africa. we identified blandm-7-producing enterobacteriaceae in gabon in 2016. the isolates contained blandm-7 incx3 plasmids that were unusual and similar to the one described in a colistin-resistant klebsiella pneumoniae sz04 isolate from china. | 2017 | 28098536 |
| rectal carriage of extended-spectrum beta-lactamase and carbapenemase producing gram-negative bacilli in intensive care units in tunisia. | this study was conducted to evaluate the rate of fecal carriage of gram-negative bacilli (gnb) resistant to third-generation cephalosporins (third gc) in patients hospitalized in the intensive care unit (icu) of charles nicolle hospital of tunis and to identify the enzymatic mechanisms involved. from february to april 2014, rectal swabs were collected from all patients (n = 38) at admission and once weekly thereafter to identify acquisition. they were cultured on desoxycholate-lactose-agar plate ... | 2017 | 28099062 |
| evaluation of three sample preparation methods for the direct identification of bacteria in positive blood cultures by maldi-tof. | patient mortality is significantly reduced by rapid identification of bacteria from sterile sites. maldi-tof can identify bacteria directly from positive blood cultures and multiple sample preparation methods are available. we evaluated three sample preparation methods and two maldi-tof score cut-off values. positive blood culture bottles with organisms present in gram stains were prospectively analysed by maldi-tof. three lysis reagents (saponin, sds, and sepsityper lysis bufer) were applied to ... | 2017 | 28100271 |
| antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates. | the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ... | 2017 | 28103015 |
| pitfalls in interpretation of fdg pet/ct: septic pulmonary emboli mimicking metastases in a case of gastric carcinoma. | inflammatory lesions may sometimes show intense tracer uptake and mimic neoplastic lesions on (18) f-fluoro-deoxyglucose (fdg) positron emission tomography/computed tomography (pet/ct). we report one such false positive case on fdg pet/ct, where septic pulmonary emboli (spe) mimicked pulmonary metastases. a 45-year-old man with stomach cancer had an indwelling central venous catheter (cvc) in situ while on neoadjuvant chemotherapy. he underwent fdg pet/ct scan for response assessment and the ima ... | 2016 | 28104952 |
| in vitro susceptibility to 19 agents other than β-lactams among third-generation cephalosporin-resistant enterobacteriaceae recovered on hospital admission. | as part of the multicentre antibiotic therapy optimisation study, mic values of 19 non-β-lactam agents were determined for third-generation cephalosporin-resistant escherichia coli, klebsiella species and enterobacter species (3gcreb) isolates collected in german hospitals. | 2017 | 28108677 |
| fungal periprosthetic joint infection following total elbow arthroplasty: a case report and review of the literature. | with improving surgical techniques for total elbow arthroplasty clinical outcomes have improved and its utilization continues to increase. despite these advances, complication rates remain as high as 24%. of these complications periprosthetic joint infection is one of the most common and morbid. the rheumatoid elbow remains a leading indication for total elbow arthroplasty. patients with this condition frequently require immunosuppressive therapy, which places them at higher risk of both typical ... | 2017 | 28109195 |
| induced membrane technique for the treatment of chronic hematogenous tibia osteomyelitis. | chronic hematogenous osteomyelitis often results from the improper treatment of acute hematogenous osteomyelitis. at present, there is lack of uniform standards for the treatment, and the clinical features of the disease are unclear. the purpose of this study was to explore the clinical efficacy and complications of chronic hematogenous tibia osteomyelitis treated with the induced membrane technique. | 2017 | 28114929 |
| comparison of 16s ribosomal rna gene sequence analysis and conventional culture in the environmental survey of a hospital. | nosocomial infection is one of the most common complications within health care facilities. certain studies have reported outbreaks resulting from contaminated hospital environments. although the identification of bacteria in the environment can readily be achieved using culturing methods, these methods detect live bacteria. sequencing of the 16s ribosomal rna (16s rrna) gene is recognized to be effective for bacterial identification. in this study, we surveyed wards where drug-resistant bacteri ... | 2017 | 28116119 |
| bioadsorption and biostabilization of cadmium by enterobacter cloacae tu. | biostabilization of cadmium, a hazardous chemical found widely in china, was attempted using enterobacter cloacae tu (e.cloacae tu). a cadmium (cd)-tolerant e.cloacae tu was obtained by mutagenesis using an atmosphere pressure glow discharge plasma system, and it displayed regular growth behavior in the presence of 250 mg/l cd in solution. the maximum stabilization capacity of e.cloacae tu toward cd reached 67.0 ± 3.5 mg/g dry cell weight at an initial cd concentration of 200 mg/l. the percentag ... | 2017 | 28117094 |
| whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental klebsiella pneumoniae. | klebsiella pneumoniae is a gram-negative bacterial species capable of occupying a broad range of environmental and clinical habitats. known as an opportunistic pathogen, it has recently become a major causative agent of clinical infections worldwide. despite growing knowledge about the highly diverse population of k. pneumoniae, the evolution and clinical significance of environmental k. pneumoniae, as well as the relationship between clinical and environmental k. pneumoniae, are poorly defined. | 2017 | 28118859 |
| optimization of enterobacter cloacae (ku923381) for diesel oil degradation using response surface methodology (rsm). | efficiency of enterobacter cloacae ku923381 isolated from petroleum hydrocarbon contaminated soil was evaluated in batch culture and bioreactor mode. the isolate were screened for biofilm formation using qualitative and quantitative assays. response surface methodology (rsm) was used to study the effect of ph, temperature, glucose concentration, and sodium chloride on diesel degradation. the predicted values for diesel oil degradation efficiency by the statistical designs are in a close agreemen ... | 2017 | 28120192 |
| effect of preoperative biliary drainage on bacterial flora in bile of patients with periampullary cancer. | patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (pbd). the effect of pbd on the microbiome of the biliary system and on postoperative outcome remains unclear. | 2017 | 28121036 |
| global assessment of the activity of tigecycline against multidrug-resistant gram-negative pathogens between 2004 and 2014 as part of the tigecycline evaluation and surveillance trial. | multidrug-resistant (mdr) gram-negative organisms are a burden on the global health care system. the tigecycline evaluation and surveillance trial (test) is an ongoing global study designed to monitor the in vitro activities of tigecycline and a panel of marketed antimicrobials against a range of clinically significant pathogens. in this study, in vitro data are presented for mdr acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterob ... | 2017 | 28124025 |
| risk factors for relapse or persistence of bacteraemia caused by enterobacter spp.: a case-control study. | enterobacter spp. possess chromosomal ampc beta-lactamases that may be expressed at high levels. previous studies have demonstrated a risk of relapsed bacteraemia following therapy with third generation cephalosporins (3gcs). what additional factors predict microbiological failure in enterobacter bacteraemia is unclear. we aimed to determine factors associated with microbiological failure in enterobacter bacteraemia. | 2017 | 28127422 |
| [profile of bacterial resistance in pediatric urinary tract infections in 2014]. | in pediatric units, bacteria-producing extended-spectrum-betalactamase (esbl) have an increasing prevalence among bacteria causing febrile urinary tract infections (utis). the purpose of this study was to evaluate the epidemiology of bacteria resistance patterns observed in utis, in order to assess the current antibiotic treatment protocols. this study is based upon a single-center retrospective chart review of the cytobacteriological urine cultures performed in utis between 1 january and 31 dec ... | 2017 | 28131557 |
| the essential oil from the fruits of the brazilian spice xylopia sericea a. st.-hil. presents expressive in-vitro antibacterial and antioxidant activity. | the aims of this study were to investigate the chemical composition and the antioxidant activity and antibacterial activity of the essential oil of xylopia sericea fruits (oxs). the fruits of this species are popularly used for medicinal purposes, and as a condiment in food preparation. | 2017 | 28134988 |
| use of colistin in a neonatal intensive care unit: a cohort study of 65 patients. | background the emergence of infections related to multidrug-resistant gram-negative bacilli (mdr-gnb) reintroduced the use of colistin, an antibiotic that was previously abandoned due to adverse effects. however, because of its limited use in neonatal intensive care units, there is very little data about the effectiveness and safety of colistin in children and newborns. in this study, which will be the largest case study in the literature, we aimed to evaluate the effectiveness and safety of col ... | 2017 | 28135233 |
| development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of listeria monocytogenes. | listeria monocytogenes, one of most problematic foodborne pathogens, is responsible for listeriosis in both humans and animals and mainly transmitted through the food chain. in this report, we propose a simple, rapid, and nearly instrument-free molecular technique using multiple cross displacement amplification (mcda) label-based gold nanoparticles lateral flow biosensor (lfb) for specific, sensitive, and visual detection of l. monocytogenes. the mcda-lfb method was carried out at a constant tem ... | 2017 | 28138243 |
| prospective surveillance of healthcare associated infections in a cambodian pediatric hospital. | healthcare associated infections (hai) are the most common preventable adverse events following admission to healthcare facilities. data from low-income countries are scarce. we sought to prospectively define hai incidence at angkor hospital for children (ahc), a cambodian pediatric referral hospital. | 2017 | 28138385 |
| prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive enterobacteriaceae in patients and healthy adults from china: an epidemiological and clinical study. | the mcr-1 gene confers transferable colistin resistance. mcr-1-positive enterobacteriaceae (mcrpe) have attracted substantial medical, media, and political attention; however, so far studies have not addressed their clinical impact. herein, we report the prevalence of mcrpe in human infections and carriage, clinical associations of mcr-1-positive escherichia coli (mcrpec) infection, and risk factors for mcrpec carriage. | 2017 | 28139431 |
| enantio convergent biotransformation of o,o-dimethyl-4-oxoazetidin-2-ylphosphonate using fungal cells of penicillium minioluteum and purified enzymes. | this report presents the bioconversion of o,o-dimethyl-4-oxoazetidin-2-ylphosphonate 1 performed in two ways: with the enzymatic system of p. minioluteum and with the application of purified enzymes: penicillinase and two proteases of different origin. recorded nmr spectra allowed confirming the reaction progress and also postulating possible mechanism of conversion. the path of bioconversion was defined as enantio convergent process for both modes of applied biocatalysts. this means that kineti ... | 2017 | 28143659 |