Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| survival of listeria monocytogenes strain h7762 and resistance to simulated gastric fluid following exposure to frankfurter exudate. | listeria monocytogenes strain h7762, a frankfurter isolate, was tested to determine whether it was able to survive at 4 degrees c in frankfurter pack fluid (exudate) and to determine whether food exposure affects its acid sensitivity. cultures were sampled and tested for acid sensitivity by challenge with simulated gastric fluid (sgf). sgf challenges performed immediately after inoculation revealed that between 20 and 26% of the cells survived the full 30 min of sgf challenge regardless of wheth ... | 2004 | 15222545 |
| efficacy of chlorine and a peroxyacetic acid sanitizer in killing listeria monocytogenes on iceberg and romaine lettuce using simulated commercial processing conditions. | the efficacy of chlorine (100 microg/ml) and a peroxyacetic acid sanitizer (80 microg/ml; tsunami 100) in killing listeria monocytogenes inoculated at populations of 1 to 2, 2 to 3, and 4 to 5 log cfu/g of iceberg lettuce pieces, shredded iceberg lettuce, and romaine lettuce pieces was determined by treatment conditions simulating those used by a commercial fresh-cut lettuce processor. the lettuce/treatment solution ratio was 1:100 (wt/vol), treatment temperature was 4 degrees c, and total treat ... | 2004 | 15222557 |
| in vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne pathogens. | the purpose of this study was to examine the effectiveness of selected essential oils for the control of growth and survival of pathogenic microorganisms of significant importance in food hygiene and to determine whether the antimicrobial effect was due to the major compounds of the oils. mic and mbc were determined by the tube dilution method. essential oils from thymus vulgaris from spain and france, salvia sclarea, salvia officinalis, salvia lavandulifolia, lavandula latifolia, lavandula angu ... | 2004 | 15222560 |
| fbpa, a novel multifunctional listeria monocytogenes virulence factor. | listeria monocytogenes is a gram-positive intracellular bacterium responsible for severe opportunistic infections in humans and animals. signature-tagged mutagenesis (stm) was used to identify a gene named fbpa, required for efficient liver colonization of mice inoculated intravenously. fbpa was also shown to be required for intestinal and liver colonization after oral infection of transgenic mice expressing human e-cadherin. fbpa encodes a 570-amino-acid polypeptide that has strong homologies t ... | 2004 | 15228540 |
| clinical and histopathological aspects of naturally occurring mastitis caused by listeria monocytogenes in cattle and ewes. | listeria monocytogenes was isolated from the milk of two cows and two sheep with mastitis in one quarter and one udder half. the animals were observed over a period of 2-12 months. clinical examination of the udder, bacteriological examinations and determination of somatic cell counts of milk samples were performed monthly. all four cases suffered from a subclinical mastitis characterized by an elevated somatic cell count (0.8-10.1 x 10(6) cells/ml), a persistent shedding of listeria and by a no ... | 2004 | 15228552 |
| a rickettsia wasp-like protein activates the arp2/3 complex and mediates actin-based motility. | spotted fever group rickettsia are obligate intracellular pathogens that exploit the host cell actin cytoskeleton to promote motility and cell-to-cell spread. although other pathogens such as listeria monocytogenes use an arp2/3 complex-dependent nucleation mechanism to generate comet tails consisting of y-branched filament arrays, rickettsia polymerize tails consisting of unbranched filaments by a previously unknown mechanism. we identified genes in several rickettsia species encoding proteins ... | 2004 | 15236643 |
| development of polythene films for food packaging activated with an antilisterial bacteriocin from lactobacillus curvatus 32y. | the aims of this work were to (i) use a bacteriocin produced by lactobacillus curvatus 32y active against listeria monocytogenes to activate polythene films by different methods, (ii) implement a large-scale process for antilisterial polythene films production and (iii) verify the efficacy of the developed films in inhibiting the growth of l. monocytogenes during the storage of meat products. | 2004 | 15239697 |
| new chromogenic plating media for detection and enumeration of pathogenic listeria spp.--an overview. | in recent years a number of selective chromogenic plating media for pathogenic listeria spp. have been developed and marketed. their advantages are direct detection and enumeration of pathogenic listeria spp. utilizing cleavage of substrates by the virulence factor phosphatidylinositol-phospholipase c (pi-plc) and, to a lesser extent, by phosphatidylcholin-phospholipase c (pc-plc). there are two groups of such media: the first utilizes cleavage by pi-plc of l-alpha-phosphatidyl-inositol, forming ... | 2004 | 15240069 |
| control of listeria monocytogenes in a biofilm by competitive-exclusion microorganisms. | biofilms from drains in food processing facilities with a recent history of no detectable listeria monocytogenes in floor drains were cultured for microorganisms producing antilisterial metabolites. a total of 413 microbial isolates were obtained from 12 drain biofilm samples and were assayed at 15 and 37 degrees c for activities that were bactericidal or inhibitory to l. monocytogenes, by two agar plate assays. twenty-one of 257 bacterial isolates and 3 of 156 yeast isolates had antilisterial a ... | 2004 | 15240275 |
| co2- and anaerobiosis-induced changes in physiology and gene expression of different listeria monocytogenes strains. | although carbon dioxide (co(2)) is known to inhibit growth of most bacteria, very little is known about the cellular response. the food-borne pathogen listeria monocytogenes is characterized by its ability to grow in high co(2) concentrations at refrigeration temperatures. we examined the listerial responses of different strains to growth in air, 100% n(2), and 100% co(2). the co(2)-induced changes in membrane lipid fatty acid composition and expression of selected genes were strain dependent. t ... | 2004 | 15240290 |
| epidemic clone i-specific genetic markers in strains of listeria monocytogenes serotype 4b from foods. | listeria monocytogenes contamination of ready-to-eat foods has been implicated in numerous outbreaks of food-borne listeriosis. however, the health hazards posed by l. monocytogenes detected in foods may vary, and speculations exist that strains actually implicated in illness may constitute only a fraction of those that contaminate foods. in this study, examination of 34 serogroup 4 (putative or confirmed serotype 4b) isolates of l. monocytogenes obtained from various foods and food-processing e ... | 2004 | 15240296 |
| natural atypical listeria innocua strains with listeria monocytogenes pathogenicity island 1 genes. | identification of bona fide listeria isolates into the six species of the genus normally requires only a few tests. aberrant isolates do occur, but even then only one or two extra confirmatory tests are generally needed for identification to species level. we have discovered a hemolytic-positive, rhamnose and xylose fermentation-negative listeria strain with surprising recalcitrance to identification to the species level due to contradictory results in standard confirmatory tests. the issue had ... | 2004 | 15240309 |
| fully functional memory cd8 t cells in the absence of cd4 t cells. | the role of cd4 t cells in providing help to cd8 t cells in primary and secondary responses to infection remains controversial. using recombinant strains of virus and bacteria expressing the same ag, we determined the requirement for cd4 t cells in endogenous cd8 t cell responses to infection with vesicular stomatitis virus and listeria monocytogenes (lm). depletion of cd4 t cells had no effect on the frequency of primary or secondary vesicular stomatitis virus-specific cd8 t cells in either lym ... | 2004 | 15240684 |
| the antimicrobial activity of extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents. | the antimicrobial activity of the chloroform, diethyl ether, acetone, petroleum ether, and ethanol extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents against 9 bacteria and fungi has been investigated. the extracts and pure compounds alone were found active against the same bacteria and the same yeasts. bacillus cereus, bacillus subtilis, staphylococcus aureus, streptococcus faecalis, proteus vulgaris, listeria monocytogenes, aero ... | 2004 | 15241936 |
| acetoin production as an indicator of growth and metabolic inhibition of listeria monocytogenes. | it has been shown that listeria monocytogenes produces acetoin from glucose under aerobic conditions. a defined medium with glucose as the sole carbon source was used in an aerobic shake flask culture to reliably produce acetoin. acetoin, the reactive compound in the voges-proskauer test, was assayable in the medium and was used to quantify the metabolic response when inhibitors were added to the medium. inhibitors such as lactic, acetic, propionic and benzoic acids were used to demonstrate the ... | 1998 | 15244053 |
| soft listeria: actin-based propulsion of liquid drops. | we study the motion of oil drops propelled by actin polymerization in cell extracts. drops deform and acquire a pearlike shape under the action of the elastic stresses exerted by the actin comet, a tail of cross-linked actin filaments. we solve this free boundary problem and calculate the drop shape taking into account the elasticity of the actin gel and the variation of the polymerization velocity with normal stress. the pressure balance on the liquid drop imposes a zero propulsive force if gra ... | 2004 | 15244616 |
| essential oils: their antibacterial properties and potential applications in foods--a review. | in vitro studies have demonstrated antibacterial activity of essential oils (eos) against listeria monocytogenes, salmonella typhimurium, escherichia coli o157:h7, shigella dysenteria, bacillus cereus and staphylococcus aureus at levels between 0.2 and 10 microl ml(-1). gram-negative organisms are slightly less susceptible than gram-positive bacteria. a number of eo components has been identified as effective antibacterials, e.g. carvacrol, thymol, eugenol, perillaldehyde, cinnamaldehyde and cin ... | 2004 | 15246235 |
| listeria monocytogenes isolated from cold-smoked fish products in osaka city, japan. | listeria monocytogenes contamination of ready-to-eat seafood products commercially available in osaka was examined between 1999 and 2000. l. monocytogenes was isolated from 12 (13%) of the 95 products tested. all positive samples were from cold-smoked fish with 9 being obtained during the summer. thirteen isolates of l. monocytogenes were typed by pulsed-field gel electrophoresis (pfge) and polymerase chain reaction (pcr)-based typing methods. isolates of the same serotype originating from the s ... | 2004 | 15246243 |
| cd8+ t cell contraction is controlled by early inflammation. | pathogen-specific cd8(+) t cells expand in number after infection and then their numbers invariably contract by 90-95%, leaving a stable memory cell pool. the chief features of this response are programmed early after infection; however, the factors regulating contraction are mostly undefined. here we show that antibiotic treatment before listeria monocytogenes infection induced numbers of protective memory cd8(+) t cells similar to those in control infected mice, by a pathway without contractio ... | 2004 | 15247915 |
| chalk and cheese. | 2004 | 15248310 | |
| looking at big brothers for clues. | 2004 | 15250878 | |
| antimicrobial effects of sanitizers against planktonic and sessile listeria monocytogenes cells according to the growth phase. | this study was designed to investigate the individual or combined effects of sanitizers on survival of planktonic or sessile listeria monocytogenes cells at different phase of growth. the sanitizers tested included: (i) acetic acid (ph 5.0), (ii) naoh (ph 12.0), (iii) 10% na2so4, (iv) 10% na2so4 and acetic acid (ph 5.0), (v) 10% na2so4 and naoh (ph 12.0), (vi) a quaternary ammonium (20 ppm) and (vii) glyceryl monolaurate (75 ppm). results revealed a great efficacy of alkaline treatments on both ... | 2004 | 15251203 |
| single-chain fv antibody with specificity for listeria monocytogenes. | single chain antibodies (scfv) exhibiting specific binding to listeria monocytogenes strains were isolated from a pool of random scfvs expressed on the surface of filamentous bacteriophages. positive selection (panning) using l. monocytogenes was used to enrich for phage clones with the desired binding affinity, and negative selection using l. innocua and l. ivanovii was used to remove phages expressing cross-reactive antibody fragments. a single phage clone, p4:a8, was selected using two indepe ... | 2004 | 15251420 |
| characterization of mesentericin st99, a bacteriocin produced by leuconostoc mesenteroides subsp. dextranicum st99 isolated from boza. | lactic acid bacteria isolated from boza, a cereal-fermented beverage from belogratchik, bulgaria, were screened for the production of bacteriocins. with the first screening, 13 of the 52 isolates inhibited the growth of listeria innocua and lactobacillus plantarum. the cell-free supernatant of one of these strains, classified as leuconostoc mesenteroides subsp. dextranicum st99, inhibited the growth of bacillus subtilis, enterococcus faecalis, several lactobacillus spp., lactococcus lactis subsp ... | 2004 | 15252717 |
| oral immunization with recombinant listeria monocytogenes controls virus load after vaginal challenge with feline immunodeficiency virus. | recombinant listeria monocytogenes has many attractive characteristics as a vaccine vector against human immunodeficiency virus (hiv). wild-type and attenuated listeria strains expressing hiv gag have been shown to induce long-lived mucosal and systemic t-cell responses in mice. using the feline immunodeficiency virus (fiv) model of hiv we evaluated recombinant l. monocytogenes in a challenge system. five cats were immunized with recombinant l. monocytogenes that expresses the fiv gag and delive ... | 2004 | 15254192 |
| genome update: annotation quality in sequenced microbial genomes. | 2004 | 15256543 | |
| intraspecific phylogeny and lineage group identification based on the prfa virulence gene cluster of listeria monocytogenes. | listeria monocytogenes is a serious food-borne pathogen that can cause invasive disease in humans and other animals and has been the leading cause of food recalls due to microbiological concerns in recent years. in order to test hypotheses regarding l. monocytogenes lineage composition, evolution, ecology, and taxonomy, a robust intraspecific phylogeny was developed based on prfa virulence gene cluster sequences from 113 l. monocytogenes isolates. the results of the multigene phylogenetic analys ... | 2004 | 15262937 |
| chemical conjugation of heterologous proteins on the surface of cowpea mosaic virus. | genetic economy leads to symmetric distributions of chemically identical subunits in icosaherdal and helical viruses. modification of the subunit genes of a variety of viruses has permitted the display of polypeptides on both the infectious virions and virus particles made in expression systems. icosahedral chimeric particles of this type often display novel properties resulting in high local concentrations of the insert. here we report an extension of this concept in which entire proteins were ... | 2004 | 15264868 |
| an update on the medical management of listeriosis. | it is still not quite well understood why there is no optimal or even a satisfactory antibiotic therapy for listeriosis. although almost all listeria strains that induce sepsis, meningitis and encephalitis, as well as many other manifestations--in particular, in immunocompromised individuals--are susceptible to most of the common antibiotics, the cure rate is only approximately 70%. the most effective regimen still consists of a combination of an aminopenicillin (amoxicillin or ampicillin) plus ... | 2004 | 15264987 |
| neutrophil involvement in cross-priming cd8+ t cell responses to bacterial antigens. | substantial cd8(+) t cell responses are generated after infection of mice with recombinant listeria monocytogenes strains expressing a model epitope (lymphocytic choriomeningitis virus np(118-126)) in secreted and nonsecreted forms. l. monocytogenes gains access to the cytosol of infected cells, where secreted ags can be accessed by the endogenous mhc class i presentation pathway. however, the route of presentation of the nonsecreted ag in vivo remains undefined. in this study we show that neutr ... | 2004 | 15265934 |
| detection and characterization of enterocins from enterococcus sp. | examination of 90 isolates of enterococcus sp. revealed production of enterocin by two isolates of e. faecalis which was inhibitory to listeria monocytogenes. two isolates of e. gallinarum produced enterocin active against staphylococcus aureus. none of the isolates antagonized salmonella enteritidis. the enterocins of e. faecalis isolates were inactivated by alpha-chymotrypsin but not by trypsin and papain, while those of e. gallinarum were resistant to all the three enzymes. enterocins produce ... | 2003 | 15266908 |
| a specific gene expression program triggered by gram-positive bacteria in the cytosol. | innate and adaptive immunity depends critically on host recognition of pathogen-associated molecules. toll-like receptors (tlrs) are key mediators of pathogen surveillance at the cell or phagocytic vacuole surface. however, mechanisms underlying recognition of pathogens in other cellular compartments remain unclear, and responses elicited by cytosolic challenge are poorly characterized. we therefore used mouse cdna microarrays to investigate gene expression triggered by infection of bone marrow- ... | 2004 | 15269347 |
| efficacy of chlorine dioxide gas as a sanitizer of lettuce leaves. | aqueous solutions of sodium hypochlorite or hypochlorous acid are typically used to sanitize fresh fruits and vegetables. however, pathogenic organisms occasionally survive aqueous sanitization in sufficient numbers to cause disease outbreaks. chlorine dioxide (clo2) gas generated by a dry chemical sachet was tested against foodborne pathogens on lettuce leaves. lettuce leaves were inoculated with cocktail of three strains each of escherichia coli o157:h7, listeria monocytogenes, and salmonella ... | 2004 | 15270488 |
| thermal process validation for escherichia coli o157:h7, salmonella, and listeria monocytogenes in ground turkey and beef products. | at 55 to 70 degrees c, thermal inactivation d-values for escherichia coli o157:h7, salmonella, and listeria monocytogenes were 19.05 to 0.038, 43.10 to 0.096, and 33.11 to 0.12 min, respectively, in ground turkey and 21.55 to 0.055, 37.04 to 0.066, and 36.90 to 0.063 min, respectively, in ground beef. the z-values were 5.73, 5.54, and 6.13 degrees c, respectively, in ground turkey and 5.43, 5.74, and 6.01 degrees c, respectively, in ground beef. in both ground turkey and beef, significant (p < 0 ... | 2004 | 15270492 |
| effect of sodium lactate on thermal inactivation of listeria monocytogenes and salmonella in ground chicken thigh and leg meat. | the effect of sodium lactate on thermal inactivation d- and z-values of listeria monocytogenes and salmonella was determined for chicken thigh and leg meat. at 55 to 70 degrees c, the d-value of l. monocytogenes in ground chicken thigh and leg meat with the addition of 4.8% sodium lactate (4.8 g sodium lactate per 100 g of meat) was 53 to 75% higher than that in the meat without sodium lactate. no significant difference was found for the d-values of salmonella at 55 to 70 degrees c between the m ... | 2004 | 15270493 |
| use of carvacrol and cymene to control growth and viability of listeria monocytogenes cells and predictions of survivors using frequency distribution functions. | the antibacterial action of carvacrol and cymene on two listeria monocytogenes strains (stcc4031 and nctn4032) was studied. carvacrol or cymene showed inhibitory effect on the growth of l. monocytogenes during lag and exponential growth phases and was more evident with increasing concentrations in brain heart infusion broth at 30 degrees c. carvacrol or cymene also decreased the survival of mid-exponential-growth-phase l. monocytogenes stcc4031 cells in potassium-n-2-hydroxy-ethylpiperazine-n-et ... | 2004 | 15270494 |
| distribution of listeria monocytogenes molecular subtypes among human and food isolates from new york state shows persistence of human disease--associated listeria monocytogenes strains in retail environments. | while there is considerable information available regarding listeria monocytogenes contamination patterns in food processing plants, our understanding of l. monocytogenes contamination and transmission in retail operations is limited. we characterized 125 food, 40 environmental, and 342 human clinical l. monocytogenes isolates collected in new york state from 1997 to 2002 using automated ribotyping and hly allelic variation. all environmental isolates were obtained from retail establishments and ... | 2004 | 15270495 |
| survival of escherichia coli o157:h7, salmonella enteritidis, staphylococcus aureus, and listeria monocytogenes in kimchi. | the survival of gram-positive and gram-negative foodborne pathogens in both commercial and laboratory-prepared kimchi (a traditional fermented food widely consumed in japan) was investigated. it was found that escherichia coli o157:h7, salmonella enteritidis, staphylococcus aureus, and listeria monocytogenes could survive in both commercial and laboratory-prepared kimchi inoculated with these pathogens and incubated at 10 degrees c for 7 days. however, when incubation was prolonged, the s. aureu ... | 2004 | 15270509 |
| the bax pcr assay for screening listeria monocytogenes targets a partial putative gene lmo2234. | the bax pcr for screening listeria monocytogenes is a commercial pcr assay for specifically targeting l. monocytogenes, a foodborne pathogen that can contaminate a variety of foods and cause a potentially fatal disease, listeriosis, among high-risk populations. the high specificity (> 98%) of this pcr assay is achieved by targeting a species-specific genomic region (approximately 400 bp) presumably found only in l. monocytogenes. in this study, the identity of the bax pcr-targeted genomic region ... | 2004 | 15270511 |
| sequence and binding activity of the autolysin-adhesin ami from epidemic listeria monocytogenes 4b. | ami is an autolytic amidase from listeria monocytogenes that is targeted to the bacterial surface via its c-terminal cell wall anchoring (cwa) domain. we recently showed that the cwa domain from ami of l. monocytogenes egd (serovar 1/2a) (ami 1/2a) mediated bacterial binding to mammalian cells. here we studied the sequence and binding properties of ami from chut 82337 (serovar 4b) (ami 4b). the ami 4b polypeptide is predicted to be 770 amino acids long (compared with the 917 amino acids of ami 1 ... | 2004 | 15271896 |
| cellular pharmacokinetics and pharmacodynamics of the glycopeptide antibiotic oritavancin (ly333328) in a model of j774 mouse macrophages. | the intracellular pharmacokinetics and pharmacodynamics of oritavancin (ly333328) were studied in cultured cells. oritavancin was avidly accumulated by j774 and thp-1 macrophages and rat fibroblasts and to a lesser extent by llc-pk1 and caco-2 cells. in j774 macrophages, the level of accumulation reached a plateau (at 370-fold the extracellular concentration) within 24 h and was partly defeated by a rise in serum protein levels. efflux was incomplete (with a plateau at two-thirds of the original ... | 2004 | 15273091 |
| application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections. | a rapid and accurate method for detection for common pathogenic bacteria in foodborne infections was established by using oligonucleotide array technology. nylon membrane was used as the array support. a mutation region of the 23s rrna gene was selected as the discrimination target from 14 species (genera) of bacteria causing foodborne infections and two unrelated bacterial species. a pair of universal primers was designed for pcr amplification of the 23s rrna gene. twenty-one species (genera)-s ... | 2004 | 15279944 |
| antimicrobial activity of reuterin in combination with nisin against food-borne pathogens. | antimicrobial activity of reuterin individually or in combination with nisin against different food-borne gram-positive and gram-negative pathogens in milk was investigated. reuterin (8 au/ml) exhibited bacteriostatic activity against listeria monocytogenes, whereas its activity was slightly bactericidal against staphylococcus aureus at 37 degrees c. higher bactericidal activity was detected against escherichia coli o157:h7, salmonella choleraesuis subsp. choleraesuis, yersinia enterocolitica, a ... | 2004 | 15282134 |
| changes in the immune functions and susceptibility to listeria monocytogenes infection in mice fed dietary lipids. | the direct examination of the effects that fish oil diets (composed of long-chain n-3 polyunsaturated fatty acids) exert on immune system function indicates a reduction of host natural resistance to infectious diseases mainly because of a suppression of immune function generated by the fatty acids contained in this diet. here, we evaluated the concentration of il-12, il-4, prostaglandin e2 and leukotriene b4 in the serum from balb/c mice receiving four different diets. each group was fed a diet ... | 2004 | 15283846 |
| thermal inactivation of salmonella and listeria monocytogenes in ground chicken thigh/leg meat and skin. | thermal inactivation d and z values of salmonella and listeria monocytogenes were obtained for chicken thigh and leg meat and skin. the d values of salmonella at 55 to 70 degrees c were 43.33 to 0.07 min in the meat and 43.76 to 0.09 min in the skin. the d values of l. monocytogenes at 55 to 70 degrees c were 38.94 to 0.04 min in the meat and 34.05 to 0.05 min in the skin. the z value of salmonella was 5.34 degrees c in the meat and 5.56 degrees c in the skin. the z value of l. monocytogenes was ... | 2004 | 15285516 |
| progress towards the use of listeria monocytogenes as a live bacterial vaccine vector for the delivery of hiv antigens. | listeria monocytogenes is a facultative intracellular bacterium that enters the cell by phagocytosis after which it colonizes the cytosol of the host cell. it is thus a potent vaccine vector for the presentation of passenger antigens to the major histocompatability complex class ii and class i pathways of antigen processing and presentation. this article shall review the progress made in developing this unusual bacterium as a vaccine vector. in mouse models, recombinant listeria carrying a numbe ... | 2004 | 15285711 |
| use of a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to high-intensity pulsed electric fields. | to apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal in food-borne bacteria exposed to high-intensity pulsed electric fields (pef). | 2004 | 15287869 |
| quantification of listeria monocytogenes in fermented sausages by mpn-pcr method. | to combine the principles of most-probable-number (mpn) statistics and the conventional pcr technique to enumerate listeria monocytogenes in fermented sausages. | 2004 | 15287877 |
| retrieving sequences of enzymes experimentally characterized but erroneously annotated : the case of the putrescine carbamoyltransferase. | annotating genomes remains an hazardous task. mistakes or gaps in such a complex process may occur when relevant knowledge is ignored, whether lost, forgotten or overlooked. this paper exemplifies an approach which could help to resuscitate such meaningful data. | 2004 | 15287962 |
| expression of mptc of listeria monocytogenes induces sensitivity to class iia bacteriocins in lactococcus lactis. | sensitivity to class iia bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (pts) permease, in listeria monocytogenes. to assess the involvement of this protein complex in class iia bacteriocin activity, the mptacd operon, encoding, was heterologously expressed in an insensitive species, namely lactococcus lactis, using the nice double plasmid system. upon induction of the cloned operon, the recombinant lc. lactis became sensitive to leucoci ... | 2004 | 15289562 |
| hypochlorite inactivation kinetics of listeria monocytogenes in phosphate buffer. | the inactivation kinetics of listeria monocytogenes in a phosphate buffer (pb) was determined at different hypochlorite concentrations, ph values and temperatures. d-values, using a linear regression, of l. monocytogenes in pb (ph 6.5) were 23.54, 17.40, 14.24 and 12.00s at 5, 10, 50 and 100 mg l(-1) hypochlorite, respectively, at 30 degrees c. the k-values ranged from 0.098 to 0.192s(-1) and 0.007 to 0.018s(-1) for hypochlorite concentrations (from 5 to 100 mg l(-1)) in pb (ph 6.5) and pb conta ... | 2004 | 15293951 |
| ecology and transmission of listeria monocytogenes infecting ruminants and in the farm environment. | a case-control study involving 24 case farms with at least one recent case of listeriosis and 28 matched control farms with no listeriosis cases was conducted to probe the transmission and ecology of listeria monocytogenes on farms. a total of 528 fecal, 516 feed, and 1,012 environmental soil and water samples were cultured for l. monocytogenes. while the overall prevalence of l. monocytogenes in cattle case farms (24.4%) was similar to that in control farms (20.2%), small-ruminant (goat and she ... | 2004 | 15294773 |
| mutational analysis of mesentericin y105, an anti-listeria bacteriocin, for determination of impact on bactericidal activity, in vitro secondary structure, and membrane interaction. | mesentericin y105 is a 37-residue bacteriocin produced by leuconostoc mesenteroides y105 that displays antagonistic activity against gram-positive bacteria such as enterococcus faecalis and listeria monocytogenes. it is closely related to leucocin a, an antimicrobial peptide containing beta-sheet and alpha-helical structures. to analyze structure-function relationships and the mode of action of this bacteriocin, we generated a collection of mesentericin derivatives. mutations were obtained mostl ... | 2004 | 15294801 |
| mechanisms regulating the positioning of mouse p47 resistance gtpases lrg-47 and iigp1 on cellular membranes: retargeting to plasma membrane induced by phagocytosis. | the recently identified p47 gtpases are one of the most effective cell-autonomous resistance systems known against intracellular pathogens in the mouse. one member of the family, lrg-47, has been shown to be essential for immune control in vivo of listeria monocytogenes, toxoplasma gondii, mycobacterium tuberculosis, and mycobacterium avium, possibly by promoting acidification of the phagosome. however, the intracellular localization of lrg-47, and the nature of its association with the phagosom ... | 2004 | 15294976 |
| cytoplasmic entry of listeria monocytogenes enhances dendritic cell maturation and t cell differentiation and function. | protective immunity to the intracellular bacterial pathogen, listeria monocytogenes, is mediated by a vigorous t cell response. in particular, cd8(+) cytolytic t cells provide essential effector function in the clearance of bacterial infection. the cytoplasmic entry of listeria facilitated by listeriolysin o is an essential feature not only of the bacteria's virulence, but of the ability of the bacteria to elicit protective immunity in the host. to determine how cytoplasmic entry of listeria reg ... | 2004 | 15294981 |
| [epidemiology of bacterial meningitis in france in 2002]. | in france, two sources of data, the mandatory notification and the hospital laboratory network epibac, allow the health authorities to monitor the incidence of bacterial meningitis. in 2002 around 1,500 cases were estimated in france. the more common microorganisms were: streptococcus pneumoniae 49% (incidence 1.19/100,000), neisseria meningitidis 33% (incidence 0.83) and streptococcus agalactiae (streptococcus b) 11% (incidence 0.27). listeria monocytogenes accounted for 4% of the cases (incide ... | 2004 | 15296270 |
| differentiation of the major listeria monocytogenes serovars by multiplex pcr. | a new multiplex pcr assay was developed to separate the four major listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, and 4b) into distinct groups. the pcr test, which constitutes a rapid and practical alternative to laborious classical serotyping, was successfully evaluated with 222 listeria strains. | 2004 | 15297538 |
| catabolite repression and virulence gene expression in listeria monocytogenes. | previous studies have suggested that carbohydrates may affect expression of virulence genes in listeria monocytogenes. which carbohydrates influence virulence gene expression and how carbohydrates mediate expression, however, is not clear. the goal of this work was to examine how carbohydrates affect virulence gene expression in l. monocytogenes 10403s. growth studies were conducted in medium containing glucose and various sugars. metabolism of arbutin, arabitol, cellobiose, mannose, maltose, tr ... | 2004 | 15297913 |
| cd4+ t cells are required for the maintenance, not programming, of memory cd8+ t cells after acute infection. | immunization in the absence of cd4(+) t cell help results in defective cd8(+) t cell memory, deficient recall responses and diminished protective immunity. here we investigated at what stage during the immune response to pathogen cd4(+) t cells are essential in the promotion of functional cd8(+) t cell memory. memory cd8(+) t cell numbers decreased gradually in the absence of cd4(+) t cells despite the presence of similar numbers of memory cell precursors at the peak of the effector phase. adopt ... | 2004 | 15300249 |
| mice lacking the type i interferon receptor are resistant to listeria monocytogenes. | listeria monocytogenes is a facultative intracellular pathogen that induces a cytosolic signaling cascade resulting in expression of interferon (ifn)-beta. although type i ifns are critical in viral defense, their role in immunity to bacterial pathogens is much less clear. in this study, we addressed the role of type i ifns by examining the infection of l. monocytogenes in balb/c mice lacking the type i ifn receptor (ifn-alpha/betar-/-). during the first 24 h of infection in vivo, ifn-alpha/beta ... | 2004 | 15302899 |
| type i interferon sensitizes lymphocytes to apoptosis and reduces resistance to listeria infection. | infection with listeria monocytogenes causes lymphocyte apoptosis that is mediated by the actions of the pore-forming virulence factor listeriolysin o (llo). previous work showed that activated lymphocytes were highly sensitive to llo-induced apoptosis, whereas resting lymphocytes were less susceptible. we now show that mice deficient in the type i interferon (ifn) receptor were more resistant to listeria infection and had less apoptotic lesions than wild-type counterparts. furthermore, treatmen ... | 2004 | 15302900 |
| type i interferon production enhances susceptibility to listeria monocytogenes infection. | numerous bacterial products such as lipopolysaccharide potently induce type i interferons (ifns); however, the contribution of this innate response to host defense against bacterial infection remains unclear. although mice deficient in either ifn regulatory factor (irf)3 or the type i ifn receptor (ifnar)1 are highly susceptible to viral infection, we show that these mice exhibit a profound resistance to infection caused by the gram-positive intracellular bacterium listeria monocytogenes compare ... | 2004 | 15302901 |
| listeria monocytogenes regulates flagellar motility gene expression through mogr, a transcriptional repressor required for virulence. | previous studies have shown that listeria monocytogenes flagellar motility genes, including flaa, encoding flagellin, are transcriptionally down-regulated at 37 degrees c. for some l. monocytogenes strains, temperature-dependent motility gene expression is less stringent. by using flaa-lacz transcriptional fusions, we identified regions upstream of the -35/-10 promoter elements that are necessary for temperature-dependent expression of flaa in l. monocytogenes strain egde. whereas the sequence o ... | 2004 | 15302931 |
| antibody-mediated activation of the classical pathway of complement may compensate for mannose-binding lectin deficiency. | deficiency of mannose-binding lectin (mbl), a recognition molecule of the lectin pathway of complement, is associated with increased susceptibility to infections. the high frequency of mbl deficiency suggests that defective mbl-mediated innate immunity can be compensated by alternative defense strategies. to examine this hypothesis, complement activation by mbl-binding ligands was studied. the results show that the prototypic mbl ligand mannan can induce complement activation via both the lectin ... | 2004 | 15307191 |
| nationwide survey of human listeria monocytogenes infection in japan. | listeriosis, caused by listeria monocytogenes, is a significant public-health concern as a result of its clinical severity and high mortality. large foodborne outbreaks of listeriosis have occurred during the last two decades in europe and the united states, but to date there have been no food-mediated epidemics of the disease and very little information is available on the number of cases of listeriosis in japan. we performed a nationwide surveillance study of listeriosis. the data were collect ... | 2004 | 15310181 |
| characteristics of the gastritis induced by listeria monocytogenes in mice: microbiology, histopathology, and mrna expression of inflammatory mediators with time course of infection. | listeria monocytogenes induces the suppurative gastritis in some mice strains. in this study, characteristics of the gastritis caused by l. monocytogenes infection in mice were examined with time course of infection. mice were administered intragastrically with 1.8 x 10(8) cfu of l. monocytogenes. each three mice were sacrificed by cervical dislocation at 1, 3, 5, 7, 10, 14, 17, 21, and 28 days postinoculation (pi), respectively. bacterial colonization in the stomachs reached the peak at 3 days ... | 2004 | 15312848 |
| cd4+cd25+ regulatory t cells that secrete tgfbeta and il-10 are preferentially induced by a vaccine vector. | cd4cd25 t cells generated in a vaccine scenario can play a critical role in limiting antitumor therapy, thus having widespread implications for the immunotherapy-based treatment of cancer. the authors previously used listeria monocytogenes to develop two vaccine constructs that express hpv-16 e7 protein and induce strong cellular immunity to hpv-e7-expressing tumors. immunization of mice bearing established e7-expressing tumors with lm-llo-e7 induced regression of the tumors, whereas lm-e7 showe ... | 2004 | 15314542 |
| splenic norepinephrine and serum corticosterone level fluctuations associated with bacteria-induced stress. | corticosterone (cort) and norepinephrine (ne), two effector molecules of the hypothalamic-pituitary-adrenal (hpa) and the sympathetic-lymphoid (sl) axes, respectively, differentially influence murine host resistance to listeria monocytogenes (lm). serum cort and splenic ne levels early (< or =24 h) after infection correlated positively with host resistance, as long as the lm burden did not exceed approximately 10(6) cfu lm per spleen. as previously reported, mice with right-circling preference ( ... | 2004 | 15316243 |
| roles of reactive oxygen species and heme oxygenase-1 in modulation of alveolar macrophage-mediated pulmonary immune responses to listeria monocytogenes by diesel exhaust particles. | diesel exhaust particles (dep) have been shown to suppress alveolar macrophage (am)-mediated pulmonary immune responses to listeria monocytogenes in vivo. in this study, effects of dep-derived reactive oxygen species (ros) and heme oxygenase (ho)-1 on am-mediated immune responses to l. monocytogenes were investigated. brown norway rats were intratracheally inoculated with 100,000 l. monocytogenes, and am were isolated at 7 days post-infection. exposure to dep or their organic extract (edep), but ... | 2004 | 15319486 |
| [hematometra & listeria monocytogenes]. | the hematometra is a nosological entity that may not always be attributed to an embryonic defect of the paramesonefros; cervical-vaginal infections such as etiological possibilities due to listeria monocytogenes (lm), cervix malignant neoplasias, iatrogenias due to endometrial ablation with lasser, traumatic bloody uterine curetage and because of cervical cryocoagulation or electrocoagulation are also mentioned. the case to be reported is from a woman in reproductive stage, who is 32 years old, ... | 2001 | 15326808 |
| immunosuppressive treatment inhibits the development of amebic liver abscesses in hamsters. | the aim of the present study was to determine if the inflammation and/or immunosuppression induced by entamoeba histolytica may contribute to amebic invasion. | 2004 | 15328476 |
| optimization of rapid detection of escherichia coli o157:h7 and listeria monocytogenes by pcr and application to field test. | for rapid detection of escherichia coli o157:h7 and listeria monocytogenes, simple methods for sample preparation and pcr were established and applied to a field test. to improve specificity, primer sets lp43-lp44 and c(+)-d(-) were selected for e. coli o157:h7 and l. monocytogenes, respectively. through centrifugation and partial heat treatment after enrichment, e. coli o157:h7 and l. monocytogenes were detected at 1 initial cfu without genomic dna extraction in the culture and with artificiall ... | 2004 | 15330527 |
| survival and recovery of viable but nonculturable listeria monocytogenes cells in a nutritionally depleted medium. | survival of a desiccated five-strain listeria monocytogenes mixture during storage in sand at 4 degrees c for 2 months was determined using the acridine orange direct count method with novobiocin and plate counts. samples of inoculated sand were taken every 2 weeks, incubated at 37 degrees c for 6 h, stained with acridine orange, and then examined with a fluorescence microscope. elongated viable but nonculturable cells were most frequently observed during weeks 2 and 4. at weeks 6 and 8, most of ... | 2004 | 15330528 |
| a validated pcr-based method to detect listeria monocytogenes using raw milk as a food model--towards an international standard. | a pcr assay with an internal amplification control was developed for listeria monocytogenes. the assay has a 99% detection probability of seven cells per reaction. when tested against 38 l. monocytogenes strains and 52 nontarget strains, the pcr assay was 100% inclusive (positive signal from target) and 100% exclusive (no positive signal from nontarget). the assay was then evaluated in a collaborative trial involving 12 european laboratories, where it was tested against an additional 14 target a ... | 2004 | 15330529 |
| high-resolution genotyping of listeria monocytogenes by fluorescent amplified fragment length polymorphism analysis compared to pulsed-field gel electrophoresis, random amplified polymorphic dna analysis, ribotyping, and pcr-restriction fragment length polymorphism analysis. | the purpose of this study was to evaluate fluorescent amplified fragment length polymorphism (aflp) analysis for the inter- and intraspecies differentiation of a collection of 96 strains of listeria monocytogenes and 10 non-l. monocytogenes strains representing six other listeria species of different origin. the aflp technique was compared with three other molecular typing methods--ribotyping, random amplified polymorphic dna analysis (rapd), and pulsed-field gel electrophoresis (pfge)--in terms ... | 2004 | 15330530 |
| thermal resistance of listeria monocytogenes, salmonella heidelberg, and escherichia coli o157:h7 at elevated temperatures. | a continuous-flow apparatus was developed to measure thermal resistance (d- and z-values) of microorganisms at temperatures above 65 degrees c. this apparatus was designed to test whether vegetative microorganisms exhibited unusually high thermal resistance that prevented them from being completely eliminated at temperatures applicable to vacuum-steam-vacuum processes (116 to 157 degrees c). the apparatus was composed of a high-pressure liquid chromatography pump, a heating unit, and a cooling u ... | 2004 | 15330531 |
| high-pressure processing of gorgonzola cheese: influence on listeria monocytogenes inactivation and on sensory characteristics. | the presence of listeria monocytogenes on the rind of gorgonzola cheese is difficult to avoid. this contamination can easily occur as a consequence of handling during ripening. the aims of this study were to determine the efficiency of high-pressure processing (hpp) for inactivation of l. monocytogenes on cheese rind and to evaluate the influence of hpp treatments on sensory characteristics. gorgonzola cheese rinds, after removal, were inoculated (about 7.0 log cfu/g) with l. monocytogenes strai ... | 2004 | 15330532 |
| fate of listeria monocytogenes in bovine manure-amended soil. | the survival and growth of listeria monocytogenes in soil amended with bovine manure was studied under different environmental conditions of temperature, nutrients, and soil microflora. autoclaved soil was compared with unautoclaved soil for assessing the influence of competitive soil microflora on the survival of l. monocytogenes. initial l. monocytogenes cell numbers of 5 to 6 log cfu/g survived for up to 43, 43, and 14 days in manure-amended autoclaved soil at 5, 15, and 21 degrees c, respect ... | 2004 | 15330533 |
| optimizing concentration and timing of a phage spray application to reduce listeria monocytogenes on honeydew melon tissue. | a phage cocktail was applied to honeydew melon pieces 1, 0.5, and 0 h before contamination with listeria monocytogenes strain lcdc 81-861 and 0.5, 1, 2, and 4 h after contamination. the phage application was most effective when applied 1, 0.5, or 0 h before contamination with l. monocytogenes, reducing pathogen populations by up to 6.8 log units after 7 days of storage. this indicates that under commercial conditions, if contamination occurs at the time of cutting, phage would have to be applied ... | 2004 | 15330534 |
| effects of high-pressure processing on the safety, quality, and shelf life of ready-to-eat meats. | ready-to-eat (rte) meats (low-fat pastrami, strassburg beef, export sausage, and cajun beef) were pressure treated at 600 mpa, 20 degrees c, for 180 s to evaluate the feasibility of using high-pressure processing (hpp) for the safe shelf-life extension of these products. after processing, samples were stored at 4 degrees c for 98 days during which time microbiological enumeration and enrichments were performed. additionally, sensory analyses were undertaken to determine consumer acceptability an ... | 2004 | 15330538 |
| exploitation of host cell cytoskeleton and signalling during listeria monocytogenes entry into mammalian cells. | deciphering how listeria monocytogenes exploits the host cell machinery to invade mammalian cells during infection isa key issue for the understanding how this food-borne pathogen causes a pleiotropic disease ranging from gastro-enteritis to meningitis and abortions. using multidisciplinary approaches, essentially combining bacterial genetics and cell biology, we have identified two bacterial proteins critical for entry into target cells, inla and inlb. their cellular ligands have been also iden ... | 2004 | 15332304 |
| republication of the article "exploitation of host cell cytoskeleton and signalling during listeria monocytogenes entry into mammalian cells". | 2004 | 15332305 | |
| [design of a hazard analysis and critical control points (haccp) plan to assure the safety of a bologna product produced by a meat processing plant]. | the hazard analysis and critical control point (haccp) is a systematic integral program used to identify and estimate the hazards (microbiological, chemical and physical) and the risks generated during the primary production, processing, storage, distribution, expense and consumption of foods. to establish a program of haccp has advantages, being some of them: to emphasize more in the prevention than in the detection, to diminish the costs, to minimize the risk of manufacturing faulty products, ... | 2004 | 15332359 |
| potential influence of the first pcr cycles in real-time comparative gene quantifications. | there is an underlying assumption in real-time pcr that the amplification efficiency is equal from the first cycles until a signal can be detected. in this study, we evaluated this assumption by analyzing genes with known gene copy number using real-time pcr comparative gene quantifications. listeria monocytogenes has six 23s rrna gene copies and one copy of the hlya gene. we determined 23s rrna gene copy numbers between 0.9 and 1.6 relative to hlya when applying the comparative gene quantificat ... | 2004 | 15335216 |
| involvement of the mpo operon in resistance to class iia bacteriocins in listeria monocytogenes. | high resistance to class iia bacteriocins in listeria monocytogenes has been clearly linked to lack of expression of the mptacd operon, encoding the eiit man mannose pts permease. also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants l. monocytogenes b73-v1 and b73-v2. we constructed a new mutant of l. monocytogenes that was interrupted in mpoa, and which also exhibited an intermediate resistance phenotype. the mpoabcd operon putati ... | 2004 | 15336400 |
| role of lipid rafts in e-cadherin-- and hgf-r/met--mediated entry of listeria monocytogenes into host cells. | listeria monocytogenes uptake by nonphagocytic cells is promoted by the bacterial invasion proteins internalin and inlb, which bind to their host receptors e-cadherin and hepatocyte growth factor receptor (hgf-r)/met, respectively. here, we present evidence that plasma membrane organization in lipid domains is critical for listeria uptake. cholesterol depletion by methyl-beta-cyclodextrin reversibly inhibited listeria entry. lipid raft markers, such as glycosylphosphatidylinositol-linked protein ... | 2004 | 15337781 |
| [effect of transferrin on the expression of virulence factors in listeria monocytogenes strains]. | iron is an essential element for the great majority of microorganisms, which developed transport systems for iron acquisition, because during the colonization and invasion processes the microorganisms encounter iron-limiting conditions. the bacterial genes implicated in the iron transport and those codifying for other virulence factors are simultaneously depressed. the purpose of this study was to investigate the role of transferrin on the virulence factors expression in food isolated listeria m ... | 2003 | 15341331 |
| a novel mutation within the central listeria monocytogenes regulator prfa that results in constitutive expression of virulence gene products. | the prfa protein of listeria monocytogenes functions as a key regulatory factor for the coordinated expression of many virulence genes during bacterial infection of host cells. prfa activity is controlled by multiple regulatory mechanisms, including an apparent requirement for either the presence of a cofactor or some form of posttranslational modification that regulates the activation of prfa. in this study, we describe the identification and characterization of a novel prfa mutation that resul ... | 2004 | 15342597 |
| rsbt and rsbv contribute to sigmab-dependent survival under environmental, energy, and intracellular stress conditions in listeria monocytogenes. | sigma b (sigma(b)) is a stress-responsive alternative sigma factor that has been identified in various gram-positive bacteria. seven different regulators of sigma b (rsbs) are located in the sigb operons of both bacillus subtilis and listeria monocytogenes. in b. subtilis, these proteins contribute to regulation of sigma(b) activity by conveying environmental and energy stress signals through two well-established branches of a signal transduction pathway. rsbt contributes to regulation of sigma( ... | 2004 | 15345420 |
| molecular fingerprinting of dairy microbial ecosystems by use of temporal temperature and denaturing gradient gel electrophoresis. | numerous microorganisms, including bacteria, yeasts, and molds, constitute the complex ecosystem present in milk and fermented dairy products. our aim was to describe the bacterial ecosystem of various cheeses that differ by production technology and therefore by their bacterial content. for this purpose, we developed a rapid, semisystematic approach based on genetic profiling by temporal temperature gradient electrophoresis (ttge) for bacteria with low-g+c-content genomes and denaturing gradien ... | 2004 | 15345452 |
| inhibition of listeria monocytogenes in fish and meat systems by use of oregano and cranberry phytochemical synergies. | optimized phenolics from oregano and cranberry extracts were evaluated for antimicrobial activity against listeria monocytogenes in laboratory media and in beef and fish. the antimicrobial activity increased when oregano and cranberry extracts were mixed at a ratio of 75% oregano and 25% cranberry (wt/wt) with 0.1 mg of phenolic per disk or ml, and the efficacy was further enhanced by lactic acid. the inhibition by phytochemical and lactic acid synergies was most effective when beef and fish sli ... | 2004 | 15345457 |
| [influence of volatile metabolites of saprophytic soil microflora on the propagation of pathogenic bacteria]. | the influence of volatile metabolites of saprophytic soil microflora on the propagation of listeria monocytogenes and yersinia pseudotuberculosis is shown. different character of interspecific relationships between bacteria, influencing their propagation, can be observed on the metabolic level. volatile compounds produced by microorganisms are capable to act as both intra- and interspecific regulators of microbial communities. in this connection the propagation of pathogenic bacteria inhabiting ... | 2004 | 15346944 |
| cell-surface alterations in class iia bacteriocin-resistant listeria monocytogenes strains. | strains of the food-borne pathogen listeria monocytogenes, showing either intermediate or high-level resistance to class iia bacteriocins, were investigated to determine characteristics that correlated with their sensitivity levels. two intermediate and one highly resistant spontaneous mutant of l. monocytogenes b73, a highly resistant mutant of l. monocytogenes 412, and a highly resistant, defined (mpta) mutant of l. monocytogenes egde were compared with their respective wild-type strains in or ... | 2004 | 15347760 |
| modulation of t cell development and activation by novel members of the schlafen (slfn) gene family harbouring an rna helicase-like motif. | the regulatory networks governing development and differentiation of hematopoietic cells are incompletely understood. members of the schlafen (slfn) protein family have been implicated in the regulation of cell growth and t cell development. we have identified and chromosomally mapped four new members, slfn5, slfn8, slfn9 and slfn10, which belong to a distinct subgroup within this gene family. the characteristic feature of these proteins is the presence of sequence motifs identifying them as dis ... | 2004 | 15351786 |
| antibacterial effect of crude water-soluble arrowroot (puerariae radix) tea extracts on food-borne pathogens in liquid medium. | to evaluate the effect of crude water-soluble arrowroot tea extracts on microbial growth of food-borne pathogens in liquid medium and to confirm the damage to bacterial cells using transmission electronic microscopy (tem). | 2004 | 15355532 |
| fas-fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta t cells. | gammadelta t cells have a direct role in resolving the host immune response to infection by eliminating populations of activated macrophages. macrophage reactivity resides within the vgamma1/vdelta6.3 subset of gammadelta t cells, which have the ability to kill activated macrophages following infection with listeria monocytogenes (lm). however, it is not known how gammadelta t cell macrophage cytocidal activity is regulated, or what effector mechanisms gammadelta t cells use to kill activated ma ... | 2004 | 15356111 |
| the development of functional cd8 t cell memory after listeria monocytogenes infection is not dependent on cd40. | the immunologic requirements for generating long-lived protective cd8 t cell memory remain unclear. memory cd8 populations generated in the absence of cd4 th cells reportedly have functional defects, and at least a subset of cd8 t cells transiently express cd40 after activation, suggesting that direct cd4-cd8 t cell interactions through cd40 may influence the magnitude and functional quality of memory cd8 populations. to ascertain the role of cd40 in such direct t cell interactions, we investiga ... | 2004 | 15356158 |
| time-temperature profiles of chilled ready-to-eat foods in school catering and probabilistic analysis of listeria monocytogenes growth. | the purpose of this study was to evaluate the chill chain in school catering by monitoring time-temperature profiles. chilled ready-to-eat foods have been chosen as subject of this study because of their high risk due to their production, storage and distribution steps, separated in time, followed by consumption without any further thermal treatment. in order to integrate the effects of storage duration and storage temperature, a quantitative criterion, namely "tte" or "time-temperature equivale ... | 2004 | 15358505 |
| molecular epidemiology and disinfectant susceptibility of listeria monocytogenes from meat processing plants and human infections. | we have investigated the molecular epidemiology of listeria monocytogenes from the meat processing industry producing cold cuts and from cases of human listeriosis by discriminative pulsed-field gel electrophoresis (pfge). a subset of the isolates was also investigated for susceptibility to a disinfectant based on quaternary ammonium compounds (qac) frequently used in the meat processing industry. the purpose of this investigation was to obtain knowledge of sources, routes of contamination and g ... | 2004 | 15358509 |
| toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. | toll-like receptor 2 (tlr2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (pg). however, studies linking pg with tlr2 recognition have relied mainly on the use of commercial staphylococcus aureus pg and have not addressed tlr2 recognition of other pg types. using highly purified pgs from eight bacteria (escherichia coli, pseudomonas aeruginosa, yersinia pseudotuberculosis, helicobacter pylori, bacillus subtilis, listeria monocytogen ... | 2004 | 15359270 |