Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| ethnopharmacological significance of eclipta alba (l.) hassk. (asteraceae). | eclipta alba can be found growing wild in fallow lands of bangladesh where it is considered as a weed by farmers. traditional medicinal systems of the indian subcontinent countries as well as tribal practitioners consider the plant to have diverse medicinal values and use it commonly for treatment of gastrointestinal disorders, respiratory tract disorders (including asthma), fever, hair loss and graying of hair, liver disorders (including jaundice), skin disorders, spleen enlargement, and cuts a ... | 2014 | 27355071 |
| circulating 25-hydroxyvitamin d and 1,25-dihydroxyvitamin d concentrations and postoperative infections in cardiac surgical patients: the calcitop-study. | vitamin d has immunomodulatory properties and seems to reduce the risk of infections. whether low vitamin d concentrations are independent risk factors for nosocomial postoperative infections in surgical patients remains to be studied in detail. | 2016 | 27355377 |
| cdi systems are stably maintained by a cell-contact mediated surveillance mechanism. | contact-dependent growth inhibition (cdi) systems are widespread amongst gram-negative bacteria where they play important roles in inter-cellular competition and biofilm formation. cdi+ bacteria use cell-surface cdia proteins to bind neighboring bacteria and deliver c-terminal toxin domains. cdi+ cells also express cdii immunity proteins that specifically neutralize toxins delivered from adjacent siblings. genomic analyses indicate that cdi loci are commonly found on plasmids and genomic islands ... | 2016 | 27355474 |
| diverse distribution of toxin-antitoxin ii systems in salmonella enterica serovars. | type ii toxin-antitoxin systems (tas), known for their presence in virulent and antibiotic resistant bacterial strains, were recently identified in salmonella enterica isolates. however, the relationships between the presence of tas (ccdab and vapbc) and the epidemiological and genetic features of different non-typhoidal salmonella serovars are largely unknown, reducing our understanding of the ecological success of different serovars. salmonella enterica isolates from different sources, belongi ... | 2016 | 27357537 |
| characteristics and management of enterobacteriaceae harboring imp-4 or imp-8 carbapenemase in a tertiary hospital. | the emergence of enterobacteriaceae harboring imp-4 or imp-8 carbapenemases is rare. we report an occurrence of enterobacteriaceae harboring imp-4 or imp-8 carbapenemases in a chinese tertiary care hospital from november 2010 to december 2012. | 2016 | 27358627 |
| [synergistic activity and clinical efficacy of fosfomycin and ciprofloxacin combination treatment for soft tissue infection caused by carbapenemase-producing enterobacter cloacae]. | 2017 | 27363666 | |
| next-generation sequencing diagnostics of bacteremia in septic patients. | bloodstream infections remain one of the major challenges in intensive care units, leading to sepsis or even septic shock in many cases. due to the lack of timely diagnostic approaches with sufficient sensitivity, mortality rates of sepsis are still unacceptably high. however a prompt diagnosis of the causative microorganism is critical to significantly improve outcome of bloodstream infections. although various targeted molecular tests for blood samples are available, time-consuming blood cultu ... | 2016 | 27368373 |
| [advances in humans and animals opportunistic pathogens from environment infecting plants by crossing kingdoms]. | some pathogenic microorganisms ubiquitous in the environment could cross kingdoms to infect diverse hosts. several cross-kingdom human pathogens were summarized in this paper, including serratia marcescens, enterobacter cloacae and pseudomonas aeuriginosa. they are ubiquitous in the nature and could cause plant diseases using the same or different infection strategies with which they infect humans and broaden host range. among these bacteria, klebsiella pneumoniae causes top rot disease of maize ... | 2016 | 27373067 |
| [clinical analysis of 67 cases of surgical site infection after spine surgery]. | to investigate the treatment of surgical site infection(ssi) after spine surgery. | 2016 | 27373479 |
| progressive increase of resistance in enterobacteriaceae urinary isolates from kidney transplant recipients over the past decade: narrowing of the therapeutic options. | antibiotic resistance is an emerging phenomenon in kidney transplantation (kt). | 2016 | 27373698 |
| disk carbapenemase test for the rapid detection of kpc-, ndm-, and other metallo-β-lactamase-producing gram-negative bacilli. | rapid detection of carbapenemase-producing gram-negative bacilli (gnb) is required for optimal treatment of infected patients. we developed and assessed a new disk carbapenemase test (dct). | 2016 | 27374708 |
| global dissemination of carbapenemase-producing klebsiella pneumoniae: epidemiology, genetic context, treatment options, and detection methods. | the emergence of carbapenem-resistant gram-negative pathogens poses a serious threat to public health worldwide. in particular, the increasing prevalence of carbapenem-resistant klebsiella pneumoniae is a major source of concern. k. pneumoniae carbapenemases (kpcs) and carbapenemases of the oxacillinase-48 (oxa-48) type have been reported worldwide. new delhi metallo-β-lactamase (ndm) carbapenemases were originally identified in sweden in 2008 and have spread worldwide rapidly. in this review, w ... | 2016 | 27379038 |
| colonization of the intestinal tract of the polyphagous pest spodoptera littoralis with the gfp-tagged indigenous gut bacterium enterococcus mundtii. | the alkaline gut of lepidopterans plays a crucial role in shaping communities of bacteria. enterococcus mundtii has emerged as one of the predominant gut microorganisms in the gastrointestinal tract of the major agricultural pest, spodoptera littoralis. therefore, it was selected as a model bacterium to study its adaptation to harsh alkaline gut conditions in its host insect throughout different stages of development (larvae, pupae, adults, and eggs). to date, the mechanism of bacterial survival ... | 2016 | 27379058 |
| plant growth promoting rhizobacteria and silicon synergistically enhance salinity tolerance of mung bean. | the present study explored the eco-friendly approach of utilizing plant-growth-promoting rhizobacteria (pgpr) inoculation and foliar application of silicon (si) to improve the physiology, growth, and yield of mung bean under saline conditions. we isolated 18 promising pgpr from natural saline soil in saudi arabia, and screened them for plant-growth-promoting activities. two effective strains were selected from the screening trial, and were identified as enterobacter cloacae and bacillus drentens ... | 2016 | 27379151 |
| optical fiber-based steady state and fluorescence lifetime spectroscopy for rapid identification and classification of bacterial pathogens directly from colonies on agar plates. | fluorescence spectroscopy was examined as a potential technique for identification and classification of bacterial pathogens. colonies of staphylococcus aureus, pseudomonas aeruginosa, salmonella typhi, and klebsiella pneumoniae on agar plates were measured directly using a laboratory spectrofluorimeter coupled with optical fiber. steady state fluorescence spectra were collected following excitation at 280 nm (tryptophan) and 380 nm (nadh). results showed that fluorescence lifetime decays of try ... | 2014 | 27379265 |
| occurrence of enterobacteriaceae in raw meat and in human samples from egyptian retail sellers. | the present study was performed to assess the presence of enterobacteriaceae in raw meat and handlers in egypt using cultivation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms). a total of 100 raw meat samples (chicken and beef meat, 50 each) were randomly purchased from butchers and local meat retailers located at mansoura city, egypt. fifty human samples were collected from meat handlers (hand swabs and stool specimens, 25 each). 228 bacterial is ... | 2014 | 27379312 |
| antibiotic combinations for controlling colistin-resistant enterobacter cloacae. | enterobacter cloacae is a gram-negative bacterium associated with high morbidity and mortality in intensive care patients due to its resistance to multiple antibiotics. currently, therapy against multi-resistant bacteria consists of using colistin, in spite of its toxic effects at higher concentrations. in this context, colistin-resistant e. cloacae strains were challenged with lower levels of colistin combined with other antibiotics to reduce colistin-associated side effects. colistin-resistant ... | 2017 | 27381521 |
| [the results and drug susceptibility of respiratory secretion culture of children with trachea bronchial foreign bodies]. | to analyze the distribution and drug resistance of the pathogenic bacteria in respiratory secretion in children with trachea bronchial foreign bodies so as to assist physicians in clinical prescription. | 2016 | 27382684 |
| the iridica bac bsi assay: rapid, sensitive and culture-independent identification of bacteria and candida in blood. | bloodstream infection (bsi) and sepsis are rising in incidence throughout the developed world. the spread of multi-drug resistant organisms presents increasing challenges to treatment. surviving bsi is dependent on rapid and accurate identification of causal organisms, and timely application of appropriate antibiotics. current culture-based methods used to detect and identify agents of bsi are often too slow to impact early therapy and may fail to detect relevant organisms in many positive cases ... | 2016 | 27384540 |
| high prevalence of plasmid-mediated quinolone resistance determinants in enterobacter cloacae isolated from hospitals of the qazvin, alborz, and tehran provinces, iran. | plasmid-mediated quinolone resistance (pmqr) is a growing clinical concern worldwide. the main aims of this study were to detect qnr-encoding genes and to evaluate the clonal relatedness of qnr-positive enterobacter cloacae isolates. | 2017 | 27384824 |
| antibacterial and antibiotic-resistance modifying activity of the extracts and compounds from nauclea pobeguinii against gram-negative multi-drug resistant phenotypes. | multi-drug resistance of gram-negative bacteria constitutes a major obstacle in the antibacterial fight worldwide. the discovery of new and effective antimicrobials and/or resistance modulators is necessary to combat the spread of resistance or to reverse the multi-drug resistance. in this study, we investigated the antibacterial and antibiotic-resistance modifying activities against 29 gram-negative bacteria including multi-drug resistant (mdr) phenotypes of the methanol extracts from nauclea p ... | 2016 | 27386848 |
| failure of the first step of two-stage revision due to polymicrobial prosthetic joint infection of the hip. | the unsuccessful treatment of prosthetic joint infection (pji) with two-stage revision leads to infection recurrence. the objectives of the study were to assess the clinical and demographic characteristics of patients with polymicrobial pji, and to evaluate the role of the microbial profile involved in pji in the risk of infection recurrence after the first step of two-stage revision surgery. | 2016 | 27387172 |
| comparison of early onset sepsis and community-acquired late onset sepsis in infants less than 3 months of age. | we compared demographic and clinical characteristics of early-onset sepsis (eos) and community-acquired late onset sepsis (ca-los) in infants. | 2016 | 27387449 |
| impact of soil salinity on the structure of the bacterial endophytic community identified from the roots of caliph medic (medicago truncatula). | in addition to being a forage crop, caliph medic (medicago truncatula) is also a model legume plant and is used for research focusing on the molecular characterization of the interaction between rhizobia and plants. however, the endophytic microbiome in this plant is poorly defined. endophytic bacteria play a role in supplying plants with the basic requirements necessary for growth and development. moreover, these bacteria also play a role in the mechanism of salinity stress adaptation in plants ... | 2016 | 27391592 |
| the synthesis of l-alanyl and β-alanyl derivatives of 2-aminoacridone and their application in the detection of clinically-important microorganisms. | in clinical microbiology the speed with which pathogenic microorganisms may be detected has a direct impact on patient health. one important strategy used in the laboratory is the growth of cultures in the presence of an enzymatic substrate which, once transformed by the appropriate microbial enzyme, generates a detectable colour or fluorescence output. such substrates have previously been prepared by our group and others and are available as commercial diagnostic kits, however they all suffer f ... | 2016 | 27391894 |
| effects of unsaturated uronic acid residues at non-reducing end on bond cleavage frequency of poly(1,4-α-l-guluronide) lyase from enterobacter cloacae m-1. | the mode of action of poly(1,4-α-l-guluronide) lyase from enterobacter cloacae m-1 on unsaturated oligoguluronic acids was studied using fluorophore-assisted carbohydrate electrophoresis. the polyguluronate lyase degraded unsaturated penta-, hexa-, and heptaguluronic acids, but not unsaturated oligoguluronic acids with dps less than 4. on comparison with the aspect of enzymatic degradation of unsaturated oligoguluronic acid and saturated oligoguluronic acid having the same dp, the former was deg ... | 1998 | 27393363 |
| antimicrobial resistance patterns of enterobacteriaceae in european wild bird species admitted in a wildlife rescue centre. | wild birds have been considered to be reservoirs of enteric human pathogens and vectors of resistance dissemination to the environment. during annual migration, they potentially play a role in the epidemiology of human associated zoonoses. the aim of this study was to investigate the frequency of isolation and antimicrobial susceptibility profiles of enterobacteriaceae members isolated from cloacal swabs of common european wild birds. fifty-five cloacal swabs were taken during birds' entrance ev ... | 2016 | 27393875 |
| high prevalence of extended-spectrum ß-lactamase producing enterobacteriaceae among clinical isolates in burkina faso. | nothing is known about the epidemiology and resistance mechanisms of extended-spectrum ß-lactamase-producing enterobacteriaceae (esbl-pe) in burkina faso. the objective of this study was to determine esbl-pe prevalence and to characterize esbl genes in burkina faso. | 2016 | 27400864 |
| comparative study of cronobacter identification according to phenotyping methods. | microbiological criteria applied to powdered infant formula (pif) require the absence of all cronobacter spp. consequently, misidentification of isolates from finished products can lead to significant financial losses for manufacturers and could increase the risk of neonatal infection. biochemical identification of suspect isolates using commercially available test panels is recommended for use by pif manufacturers by both the us fda and iso standard methods for cronobacter species; however, phe ... | 2016 | 27401027 |
| interaction of avibactam with class b metallo-β-lactamases. | β-lactamases are the most important mechanisms of resistance to the β-lactam antibacterials. there are two mechanistic classes of β-lactamases: the serine β-lactamases (sbls) and the zinc-dependent metallo-β-lactamases (mbls). avibactam, the first clinically useful non-β-lactam β-lactamase inhibitor, is a broad-spectrum sbl inhibitor, which is used in combination with a cephalosporin antibiotic (ceftazidime). there are multiple reports on the interaction of avibactam with sbls but few such studi ... | 2016 | 27401561 |
| small intestinal bacterial overgrowth is associated with irritable bowel syndrome and is independent of proton pump inhibitor usage. | current knowledge suggests that small intestinal overgrowth participates in the pathogenesis of irritable bowel syndrome. it is questionable if this association is modulated by intake of proton pump inhibitors (ppis). | 2016 | 27402085 |
| identification of an ndm-5-producing escherichia coli sequence type 167 in a neonatal patient in china. | emergence of new delhi metallo-β-lactamase-producing enterobacteriaceae has become a challenging threat to public health. two carbapenem-resistant escherichia coli, strain qd28 and qd29, were recovered from the aspirating sputum of a neonate and the urine of an adult in a chinese hospital in 2013. molecular typing revealed that both isolates belonged to the sequence type 167, but they were clonally diverse. both isolates exhibited resistance to carbapenems, cephalosporins, ciprofloxacin, gentami ... | 2016 | 27406405 |
| bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico. | currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ... | 2016 | 27407294 |
| bartonella henselae as a putative cause of congenital cholestasis. | severe anemia and cholestatic hepatitis are associated with bartonella infections. a putative vertical bartonella henselae infection was defined on the basis of ultrastructural and molecular analyses in a three-year-old child with anemia, jaundice and hepatosplenomegaly since birth. physicians should consider bartonellosis in patients with anemia and hepatitis of unknown origin. | 2016 | 27410916 |
| preventing data ambiguity in infectious diseases with four-dimensional and personalized evaluations. | diagnostic errors can occur, in infectious diseases, when anti-microbial immune responses involve several temporal scales. when responses span from nanosecond to week and larger temporal scales, any pre-selected temporal scale is likely to miss some (faster or slower) responses. hoping to prevent diagnostic errors, a pilot study was conducted to evaluate a four-dimensional (4d) method that captures the complexity and dynamics of infectious diseases. | 2016 | 27411058 |
| in vitro activity of tigemonam against multiresistant nosocomial enterobacteriaceae. | tigemonam, an oral monobactam that exhibits beta-lactamase stability similar to that of aztreonam, was tested in vitro against 240 species of enterobacteriaceae (50 escherichia coli, 48 klebsiella pneumoniae, 52 enterobacter cloacae, 32 proteus mirabilis, 22 proteus indole-positive [providencia sp.], 24 serrada sp., and 12 citrobacter sp. all strains were resistant to ampicillin and first-generation cephalosporins. in addition, 77.4% were resistant to amoxicillin plus clavulanic acid, 46.8% to c ... | 1989 | 27416147 |
| antibacterial activity in vitro of tigemonam, a new oral monobactam. | using a broth microdilution method, the antibacterial activity of tigemonam, a novel oral monobactam, was evaluated against 217 gram-negative aerobes freshly isolated from clinical specimems. reference antibiotics were amoxicillin, amoxicillin-sulbactam, cephalexin, cefaclor, norfloxacin, and co-trimoxazole. at a concentration of ≦ 8 mg/l, tigemonam inhibited 88% of the members of the enterobacteriaceae family. its activity was poor against the nonfermenter pathogens. in comparison with the othe ... | 1989 | 27416149 |
| bactericidal activity of tigemonam, alone and in combination with gentamicin. | the in vitro bactericidal activity of tigemonam, alone and in combination with gentamicin, was evaluated against various strains of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, salmonella enteritidis, enterobacter cloacae, proteus mirabilis, and proteus vulgaris. minimum inhibitory and bactericidal concentrations were determined on three consecutive days with an inoculum of 10(5) colony-forming units (cfu)/ml or a culture in the logarithmic growth phase. the results found tigemon ... | 1989 | 27416150 |
| interferon-inducible guanylate-binding proteins at the interface of cell-autonomous immunity and inflammasome activation. | guanylate-binding proteins (gbps) are essential components of cell-autonomous immunity. in response to ifn signaling, gbps are expressed in the cytoplasm of immune and nonimmune cells, where they unleash their antimicrobial activity toward intracellular bacteria, viruses, and parasites. recent studies have revealed that gbps are essential for mediating activation of the caspase-1 inflammasome in response to the gram-negative bacteria salmonella enterica serovar typhimurium, francisella novicida, ... | 2017 | 27418355 |
| prevalence of extended spectrum beta-lactamase production among uropathogens in south mumbai and its antibiogram pattern. | β-lactams are the most widely used group of antimicrobials. however, increasing resistance to these valuable drugs in uropathogens, mediated principally by β-lactamases, has become a major concern. the present study was undertaken to determine the prevalence of extended spectrum β-lactamase (esbl) producers in clinical isolates of urine specimens, collected from various healthcare centres across south mumbai. a total of 195 gram negative urine isolates were identified as pseudomonas aeruginosa ( ... | 2012 | 27418912 |
| clinical characteristics and predictors of adverse outcome in adult and pediatric patients with healthcare-associated ventriculitis and meningitis. | background. healthcare-associated meningitis or ventriculitis is a serious and life-threatening complication of invasive neurosurgical procedures or penetrating head trauma. methods. we performed a retrospective study of adults and children with the diagnosis of healthcare-associated meningitis or ventriculitis, as defined by the 2015 centers of disease control and prevention case definition, at 2 large tertiary care hospitals in houston, texas from july 2003 to november 2014. patients were id ... | 2016 | 27419154 |
| metallo-β-lactamase (mbl)-producing enterobacteriaceae in united states children. | metallo-β-lactamases (mbls) are emerging as the most notable resistance determinants in enterobacteriaceae. in many cases, the genes encoding mbls are part of complex, mobile genetic elements that carry other resistance determinants. in the united states, there are increasing reports of mbl-producing enterobacteriaceae, with new delhi mbls (ndms) accounting for the majority of transmissible mbl infections. many infections caused by ndm-producing bacteria are associated with international travel ... | 2016 | 27419164 |
| high prevalence of plasmid-mediated quinolone resistance and incq plasmids carrying qnrs2 gene in bacteria from rivers near hospitals and aquaculture in china. | effluents from hospital and aquaculture are considered important sources of quinolone resistance. however, little information is available on the impact of this effluent on nearby rivers. in this study, 188 ciprofloxacin-resistant bacterial isolates obtained from rivers near hospitals and aquaculture were screened for plasmid-mediated quinolone resistance (pmqr) genes. species identification, antibiotic susceptibility testing, and pmqr gene transferability assessment were conducted for pmqr-posi ... | 2016 | 27427763 |
| outbreak of achromobacter xylosoxidans and ochrobactrum anthropi infections after prostate biopsies, france, 2014. | we report an outbreak of healthcare-associated prostatitis involving rare environmental pathogens in immunocompetent patients undergoing transrectal prostate biopsies at hôpital édouard herriot (lyon, france) during august 13-october 10, 2014. despite a fluoroquinolone-based prophylaxis, 5 patients were infected with achromobacter xylosoxidans and 3 with ochrobactrum anthropi, which has not been reported as pathogenic in nonimmunocompromised persons. all patients recovered fully. analysis of the ... | 2016 | 27434277 |
| peptidomics of acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity. | acanthoscurria gomesiana is a brazilian spider from the theraphosidae family inhabiting regions of southeastern brazil. potent antimicrobial peptides as gomesin and acanthoscurrin have been discovered from the spider hemolymph in previous works. spider venoms are also recognized as sources of biologically active peptides, however the venom peptidome of a. gomesiana remained unexplored to date. in this work, a ms-based workflow was applied to the investigation of the spider venom peptidome. data- ... | 2017 | 27436114 |
| spread of enterobacter cloacae carrying blandm-1, blactx-m-15, blashv-12 and plasmid-mediated quinolone resistance genes in a surgical intensive care unit in croatia. | the objective of this study was to describe a hospital cluster of ndm-1-producing enterobacter cloacae infections observed in the surgical intensive care unit (icu) of a tertiary-care hospital at pula, croatia. ndm-1-producing e. cloacae strains isolated from clinical samples were screened by pcr for the presence of carbapenemases. genetic relatedness of ndm-1-producing e. cloacae strains was determined by multilocus sequence typing (mlst). during the period october 2013 to april 2014, four pati ... | 2016 | 27436392 |
| imi-1-producing enterobacter cloacae clinical isolate from tahiti, french polynesia. | 2016 | 27436456 | |
| treatment of polymicrobial osteomyelitis with ceftolozane-tazobactam: case report and sensitivity testing of isolates. | stenotrophomonas maltophilia is an inherently multidrug resistant (mdr) opportunistic pathogen with many mechanisms of resistance. sentry studies reveal decreasing sensitivities of s. maltophilia to trimethoprim-sulfamethoxazole and fluoroquinolones. ceftolozane-tazobactam (zerbaxa, merck & co., inc.) a novel intravenous combination agent of a third-generation cephalosporin and β-lactamase inhibitor was demonstrated to have in vitro activity against many gram-positive, gram-negative, and mdr org ... | 2016 | 27437155 |
| "omics" tools for better understanding the plant-endophyte interactions. | endophytes, which mostly include bacteria, fungi and actinomycetes, are the endosymbionts that reside asymptomatically in plants for at least a part of their life cycle. they have emerged as a valuable source of novel metabolites, industrially important enzymes and as stress relievers of host plant, but still many aspects of endophytic biology are unknown. functions of individual endophytes are the result of their continuous and complex interactions with the host plant as well as other members o ... | 2016 | 27446181 |
| the epidemiologic, microbiologic and clinical picture of bacteremia among febrile infants and young children managed as outpatients at the emergency room, before and after initiation of the routine anti-pneumococcal immunization. | we described the occult bacteremia (ob) and bacteremia with diagnosed focus (bwf) picture among children managed as outpatients at the pediatric emergency room (per) in southern israel, before and after the introduction of pneumococcal conjugate vaccines (pcvs) introduction in a retrospective study enrolling all three- to 36-month-old patients with fever >38.0 °c during 2005-2014. of 511 (0.82% of all febrile patients) true bacteremias, 230 (45%) were managed as outpatients; 96 of 230 (41.7%) ha ... | 2016 | 27447651 |
| biliary microbiota, gallstone disease and infection with opisthorchis felineus. | there is increasing interest in the microbiome of the hepatobiliary system. this study investigated the influence of infection with the fish-borne liver fluke, opisthorchis felineus on the biliary microbiome of residents of the tomsk region of western siberia. | 2016 | 27447938 |
| silver nanoparticles production by two soil isolated bacteria, bacillus thuringiensis and enterobacter cloacae, and assessment of their cytotoxicity and wound healing effect in rats. | production of silver nanoparticles by bacillus thuringiensis and enterobacter cloacae was performed and confirmed through uv-visible spectrophotometry, transmission electron microscopy (tem), and x-ray diffraction (xrd) analyses. the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay using mouse fibroblast cell line nih-3t3 d4 was carried out and ic50s of agnps were obtained. the nontoxic dose of each agnps solution was selected for wound healing assay. thirty-two rats were ... | 2016 | 27448276 |
| the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members. | the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members. | 2016 | 27448413 |
| mobilization of blabkc-1 by iskpn23? | 2016 | 27451437 | |
| erratum for norgan et al., carbapenem- and colistin-resistant enterobacter cloacae from delta, colorado, in 2015. | 2016 | 27451439 | |
| late-onset deep mesh infection: a study of eight cases detected from 2666 consecutive patients with abdominal wall hernia repairs. | 2016 | 27453240 | |
| imperatorin-pharmacological meaning and analytical clues: profound investigation. | imperatorin, a furanocoumarin derivative, has many documented pharmacological properties which make it a candidate for possible drug development. in this review, the activity on the central nervous system, the anticancer and antiviral properties and the influence on the cardiovascular system are described. the aim of this review is also to present an overview of the techniques used for the analysis, isolation, and separation of imperatorin from plant material from the practical perspective. | 2016 | 27453708 |
| first report of german cockroaches (blattella germanica) as reservoirs of ctx-m-15 extended-spectrum-β-lactamase- and oxa-48 carbapenemase-producing enterobacteriaceae in batna university hospital, algeria. | here we report the isolation of extended-spectrum β-lactamase (esbl)- and carbapenemase-producing enterobacteriaceae from german cockroaches caught in the burn unit of batna university hospital in algeria. nine of 12 isolates harbored the blactx-m-15 esbl gene. one enterobacter cloacae isolate belonging to sequence type 528 coexpressed the blaoxa-48, blactx-m-15, and blatem genes. our findings indicate that cockroaches may be one of the most dangerous reservoirs for esbl and carbapenemase produc ... | 2016 | 27458214 |
| clonal transmission of gram-negative bacteria with carbapenemases ndm-1, vim-1, and oxa-23/72 in a bulgarian hospital. | we characterized 72 isolates with reduced susceptibility to carbapenems (50 acinetobacter spp., 13 proteus mirabilis, five escherichia coli, one morganella morganii, one enterobacter cloacae, one providencia rettgeri, and one pseudomonas aeruginosa) from a hospital in sofia, bulgaria. different β-lactamase genes were identified by polymerase chain reaction and sequencing. bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (pfge) typing as wel ... | 2017 | 27459019 |
| outbreak of extended-spectrum beta-lactamase producing enterobacter cloacae with high mics of quaternary ammonium compounds in a hematology ward associated with contaminated sinks. | to investigate an outbreak of extended-spectrum beta-lactamase (esbl) producing enterobacter cloacae that occurred in the hematology ward (24-bed unit) of the françois mitterrand university hospital (dijon, france) between january 2011 and december 2013. the outbreak involved 43 patients (10 infected and 33 colonized). | 2016 | 27462306 |
| the delta high-density lipoprotein cholesterol ratio: a novel parameter for gram-negative sepsis. | to study changes in blood lipid metabolism in sepsis patients, especially high-density lipoprotein cholesterol (hdl-c) changes in the diagnosis of sepsis and the type of bacteria involved. | 2016 | 27462492 |
| the iridica pcr/electrospray ionization-mass spectrometry assay on bronchoalveolar lavage for bacterial etiology in mechanically ventilated patients with suspected pneumonia. | we studied the diagnostic performance of the iridica pcr/electrospray ionization-mass spectrometry (pcr/esi-ms) assay applied on bronchoalveolar lavage (bal) samples, from 51 mechanically ventilated patients with suspected pneumonia, in a prospective study. in 32 patients with x-ray verified pneumonia, pcr/esi-ms was positive in 66% and bal culture was positive in 38% (p = 0.045), and either of the methods was positive in 69%. the following bal result combinations were noted: pcr/esi-ms+/culture ... | 2016 | 27463099 |
| citrobacter freundii carrying blakpc-2 and blandm-1: characterization by whole genome sequencing. | a carbapenem-resistant citrobacter freundii strain wchcf65 was recovered from hospital sewage and was characterized by genome sequencing and conjugation experiments. the strain carried nine genes encoding β-lactamases including two carbapenemase genes, blandm-1 and blakpc-2. blandm-1 was carried on an incx3 plasmid, which was identical to a plasmid found in a local escherichia coli, suggesting interspecies horizontal transfer. blakpc-2 was bracketed by two copies of insertion sequence iskpn19, w ... | 2016 | 27465241 |
| selective intestinal decontamination for the prevention of early bacterial infections after liver transplantation. | bacterial infection in the first month after liver transplantation is a frequent complication that poses a serious risk for liver transplant recipients as contributes substantially to increased length of hospitalization and hospital costs being a leading cause of death in this period. most of these infections are caused by gram-negative bacilli, although gram-positive infections, especially enterococcus sp. constitute an emerging infectious problem. this high rate of early postoperative infectio ... | 2016 | 27468189 |
| strategies for prevention of ultrasound-guided prostate biopsy infections. | prostate cancer is the most common cancer in male patients and the second leading cause of cancer-related mortality in males. to confirm the diagnosis of prostate cancer, an ultrasound-guided needle biopsy is necessary to obtain prostate tissue sufficient for histologic analysis by pathologists. ultrasound-guided prostate needle biopsy can be accomplished via a transperineal or transrectal approach. the latter biopsy technique involves placing an ultrasound probe into the rectum, visualizing the ... | 2016 | 27468242 |
| endonuclease restriction-mediated real-time polymerase chain reaction: a novel technique for rapid, sensitive and quantitative detection of nucleic-acid sequence. | the article reported a novel methodology for real-time pcr analysis of nucleic acids, termed endonuclease restriction-mediated real-time polymerase chain reaction (et-pcr). just like pcr, et-pcr only required one pair of primers. a short sequence, which was recognized by restriction enzyme bstui, was attached to the 5' end of the forward (f) or reverse (r) pcr primer, and the new f or r primer was named ef or er. ef/er was labeled at the 5' end with a reporter dye and in the middle with a quench ... | 2016 | 27468284 |
| scarcity of autoreactive human blood iga(+) memory b cells. | class-switched memory b cells are key components of the "reactive" humoral immunity, which ensures a fast and massive secretion of high-affinity antigen-specific antibodies upon antigenic challenge. in humans, iga class-switched (iga(+) ) memory b cells and iga antibodies are abundant in the blood. although circulating iga(+) memory b cells and their corresponding secreted immunoglobulins likely possess major protective and/or regulatory immune roles, little is known about their specificity and ... | 2016 | 27469325 |
| clinical relevance of pathogens detected by multiplex pcr in blood of very-low-birth weight infants with suspected sepsis - multicentre study of the german neonatal network. | in the german neonatal network (gnn) 10% of very-low-birth weight infants (vlbwi) suffer from blood-culture confirmed sepsis, while 30% of vlbwi develop clinical sepsis. diagnosis of sepsis is a difficult task leading to potential over-treatment with antibiotics. this study aims to investigate whether the results of blood multiplex-pcr (septifast®) for common sepsis pathogens are relevant for clinical decision making when sepsis is suspected in vlbwi. | 2016 | 27472282 |
| risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry. | the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ... | 2017 | 27478029 |
| physicochemical and antibacterial properties of chitosan extracted from waste shrimp shells. | this research aims to study the production of chitosan from shrimp shell (litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. the evaluation of the quality of chitosan from waste shrimp shells includes parameters for the yield, physical chemistry characteristics by infrared spectroscopy (ft-ir), the degree of deacetylation, and antibacterial activity. the results showed (by method 1) extraction yiel ... | 2016 | 27478443 |
| bioactivity of polyphenols: preventive and adjuvant strategies toward reducing inflammatory bowel diseases-promises, perspectives, and pitfalls. | inflammatory bowel diseases (ibds) are characterized by autoimmune and inflammation-related complications of the large intestine (ulcerative colitis) and additional parts of the digestive tract (crohn's disease). complications include pain, diarrhoea, chronic inflammation, and cancer. ibd prevalence has increased during the past decades, especially in westernized countries, being as high as 1%. as prognosis is poor and medication often ineffective or causing side effects, additional preventive/a ... | 2016 | 27478535 |
| effect of 4 % chlorhexidine on cord colonization among hospital and community births in india: a randomized controlled study. | infections are the single most important cause of neonatal mortality in developing countries. results from trials in asia evaluating the effect of chlorhexidine on neonatal mortality have been encouraging but limited data are available on the impact of cord cleansing on bacterial colonization. further, no data from facility deliveries and impact with time is available. this pilot study was aimed to evaluate the impact of 4 % commercially prepared chlorhexidine on cord colonization and density of ... | 2016 | 27484013 |
| resistant mechanisms and molecular epidemiology of imipenem-resistant acinetobacter baumannii. | the aim of the study was to investigate the resistant mechanisms and homology of imipenem-resistant acinetobacter baumannii (a. baumannii). a total of 46 non-duplicate imipenem‑resistant a. baumannii clinical isolates were collected from three tertiary hospitals between july, 2011 and june, 2012. the minimal inhibitory concentrations (mics) of antimicrobial agents were determined using the agar dilution method. phenylalanine‑arginine β-naphthylamide was used to detect the presence of the efflux ... | 2016 | 27485638 |
| catheter-related blood stream infection in patients receiving long-term home parenteral nutrition: tertiary care hospital experience in saudi arabia. | parenteral nutrition (pn) is a lifesaving therapy for patients with many severe conditions, including intestinal failure. some patients require long-term pn therapy, which makes home parenteral nutrition (hpn) an attractive option to improve the quality of life. among the most common and serious complications observed in these patients are catheter-related blood stream infections (crbsis). the aim of our study is to determine the frequency of crbsi among patients receiving long-term hpn. | 2016 | 27488325 |
| febrile neutropenia in children with acute lymphoblastic leukemia: single center experience. | an important life-threatening complication of intensive chemotherapy administered in children with leukemia is febrile neutropenia. the objective of this study was to evaluate the clinical features and consequences of febrile neutropenia attacks in children who were treated for acute lymphoblastic leukemia. | 2016 | 27489464 |
| phlegmonous gastritis secondary to superior mesenteric artery syndrome. | we herein report a case of phlegmonous gastritis secondary to superior mesenteric artery syndrome. an 80-year-old woman visited the hospital emergency department with the chief complaints of epigastric pain and vomiting. she was hospitalized urgently following the diagnosis of superior mesenteric artery syndrome based on abdominal computed tomography findings. conservative therapy was not effective, and phlegmonous gastritis was diagnosed based on the findings of upper gastrointestinal endoscopy ... | 2015 | 27489695 |
| real-time quantitative pcr assay with taqman(®) probe for rapid detection of mcr-1 plasmid-mediated colistin resistance. | here we report the development of two rapid real-time quantitative pcr assays with taqman(®) probes to detect the mcr-1 plasmid-mediated colistin resistance gene from bacterial isolates and faecal samples from chickens. specificity and sensitivity of the assay were 100% on bacterial isolates including 18 colistin-resistant isolates carrying the mcr-1 gene (six klebsiella pneumoniae and 12 escherichia coli) with a calibration curve that was linear from 10(1) to 10(8) dna copies. five out of 833 f ... | 2016 | 27489722 |
| fructose 1,6-bisphosphate aldolase, a novel immunogenic surface protein on listeria species. | listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. increased understanding of the surface exposed antigens on listeria would provide potential diagnostic and therapeutic targets. in the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (fba) class ii in listeria species is the antigen target of the previously described mab-3f8. western and ... | 2016 | 27489951 |
| a locking compression plate as an external fixator for treating infected nonunion of the humeral diaphysis. | infected nonunion of the humeral diaphysis is a challenging problem for orthopedic surgeons. this study aimed to evaluate the outcome of using a locking compression plate (lcp) as a definitive external fixator in the management of infected nonunion of the humeral diaphysis after failure of internal fixation. | 2016 | 27491400 |
| characterization of extended-spectrum β-lactamase (esbl)-producing klebsiella, enterobacter, and citrobacter obtained in environmental samples of a tunisian hospital. | the assessment of the hospital environment as a reservoir of esbl-producing enterobacteriaceae in tunisian hospitals is scarcely analyzed, except for escherichia coli. the aim of this study was to evaluate the presence of esbl-producing non-e. coli enterobacteriaceae (esbl-ebnoec) in 300 samples of abiotic surfaces and the hands of patients and staff of a tunisian hospital, and to characterize the esbl genes of the recovered isolates. esbl-ebnoec were recovered in 28 of 300 (9.3%) analyzed sampl ... | 2016 | 27492133 |
| innovative approaches using lichen enriched media to improve isolation and culturability of lichen associated bacteria. | lichens, self-supporting mutualistic associations between a fungal partner and one or more photosynthetic partners, also harbor non-photosynthetic bacteria. the diversity and contribution of these bacteria to the functioning of lichen symbiosis have recently begun to be studied, often by culture-independent techniques due to difficulties in their isolation and culture. however, culturing as yet unculturable lichenic bacteria is critical to unravel their potential functional roles in lichen symbi ... | 2016 | 27494030 |
| esbl detection: comparison of a commercially available chromogenic test for third generation cephalosporine resistance and automated susceptibility testing in enterobactericeae. | rapid detection and reporting of third generation cephalosporine resistance (3gc-r) and of extended spectrum betalactamases in enterobacteriaceae (esbl-e) is a diagnostic and therapeutic priority to avoid inefficacy of the initial antibiotic regimen. in this study we evaluated a commercially available chromogenic screen for 3gc-r as a predictive and/or confirmatory test for esbl and ampc activity in clinical and veterinary enterobacteriaceae isolates. the test was highly reliable in the predicti ... | 2016 | 27494134 |
| cluster-dependent colistin hetero-resistance in enterobacter cloacae complex. | aims of this study were to: (i) evaluate whether the cluster membership could have an impact on hetero-resistance phenotype to colistin in the enterobacter cloacae complex (ecc); and (ii) determine the genetic mechanism of colistin hetero-resistance in ecc. | 2016 | 27494907 |
| hydroxylation of diverse flavonoids by cyp450 bm3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities. | cytochrome p450 monooxygenase constitutes a significant group of oxidative enzymes that can introduce an oxygen atom in a high regio- and stereo-selectivity mode. we used the bacillus megaterium cytochrome p450 bm3 (cyp450 bm3) and its variants namely mutant 13 (m13) and mutant 15 (m15) for the hydroxylation of diverse class of flavonoids. | 2016 | 27495155 |
| tigecycline-based versus sulbactam-based treatment for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex. | the treatment options for pneumonia involving multidrug-resistant acinetobacter calcoaceticus-acinetobacter baumannii (mdr acb) complex are limited, and the optimal treatment has not been established. | 2016 | 27496018 |
| comparison of methods for the identification of microorganisms isolated from blood cultures. | bloodstream infections are responsible for thousands of deaths each year. the rapid identification of the microorganisms causing these infections permits correct therapeutic management that will improve the prognosis of the patient. in an attempt to reduce the time spent on this step, microorganism identification devices have been developed, including the vitek(®) 2 system, which is currently used in routine clinical microbiology laboratories. | 2016 | 27496125 |
| association between chronic hemodialysis and bloodstream infections caused by chromosomally mediated ampc-producing enterobacteriaceae. | the combination of inherent antimicrobial resistance and high mortality after bloodstream infections (bsis) caused by chromosomally mediated ampc-producing enterobacteriaceae (cae) emphasizes the importance of identifying patients at risk of bsi because of these bacteria. this retrospective case-control study examines chronic hemodialysis among other risk factors for bsi caused by cae. | 2016 | 27499192 |
| carriage of multidrug-resistant organisms in a tertiary university hospital in albania-a point prevalence survey. | antimicrobial resistance has been recognised as a serious global public health problem. prevalence of multiple-drug-resistant (mdr) organism carriage in albania is largely unknown since no national surveillance system is in place and few publications are accessible in the literature. | 2016 | 27499852 |
| risk factors to predict drug-resistant pathogens in hemodialysis-associated pneumonia. | after the concept of healthcare associated pneumonia (hcap) was introduced in 2005 by the american thoracic society/infectious disease society of america (ats/idsa), pneumonia in hemodialysis patients has been classified as hcap. even though there are several risk factors and scoring systems of drug-resistant pathogens (drps) in hcap, the risk factors for drps in hemodialysis-associated pneumonia are unclear. | 2016 | 27502599 |
| prospective evaluation of septifast multiplex pcr in children with systemic inflammatory response syndrome under antibiotic treatment. | antimicrobially pre-treated children with systemic inflammation often pose a diagnostic challenge to the physician. we aimed to evaluate the additional use of septifast multiplex polymerase chain reaction (pcr) to identify causative pathogens in children with suspected systemic bacterial or fungal infection. | 2016 | 27503068 |
| salmonella typhimurium utilizes a t6ss-mediated antibacterial weapon to establish in the host gut. | the mammalian gastrointestinal tract is colonized by a high-density polymicrobial community where bacteria compete for niches and resources. one key competition strategy includes cell contact-dependent mechanisms of interbacterial antagonism, such as the type vi secretion system (t6ss), a multiprotein needle-like apparatus that injects effector proteins into prokaryotic and/or eukaryotic target cells. however, the contribution of t6ss antibacterial activity during pathogen invasion of the gut ha ... | 2016 | 27503894 |
| ceftazidime-avibactam (avycaz): for the treatment of complicated intra-abdominal and urinary tract infections. | ceftazidime-avibactam (avycaz) for the treatment of complicated infections. | 2016 | 27504064 |
| a retrospective analysis of antimicrobial resistance in bacterial pathogens in an equine hospital (2012-2015). | antimicrobial resistance has become an important concern in veterinary medicine. the aim of this study was to describe the rate of antimicrobial resistance in common equine pathogens and to determine the occurrence of multidrug-resistant isolates. a retrospective analysis of all susceptibility testing results from bacterial pathogens cultured from horses at the university of zurich equine hospital (2012-2015) was performed. strains exhibiting resistance to 3 or more antimicrobial categories were ... | 2016 | 27504838 |
| interaction of staphylococcus aureus persister cells with the host when in a persister state and following awakening. | persister cells, a tolerant cell sub-population, are commonly associated with chronic and recurrent infections. however, little is known about their ability to actually initiate or establish an infection, become virulent and cause pathogenicity within a host. here we investigated whether staphylococcus aureus persister cells initiate an infection and are recognized by macrophages, while in a persister cell status, and upon awakening due to exposure to cis-2-decenoic acid (cis-da). our results sh ... | 2016 | 27506163 |
| multidrug-resistant surgical site infections in a humanitarian surgery project. | the epidemiology of surgical site infections (ssis) in surgical programmes in sub-saharan africa is inadequately described. we reviewed deep and organ-space ssis occurring within a trauma project that had a high-quality microbiology partnership and active follow-up. included patients underwent orthopaedic surgery in teme hospital (port harcourt, nigeria) for trauma and subsequently developed a ssi requiring debridement and microbiological sampling. data were collected from structured chart revie ... | 2016 | 27509824 |
| the etiology, incidence, and impact of preservation fluid contamination during liver transplantation. | the role of contaminated preservation fluid in the development of infection after liver transplantation has not been fully elucidated. to assess the incidence and etiology of contaminated preservation fluid and determine its impact on the subsequent development of infection after liver transplantation, we prospectively studied 50 consecutive liver transplants, and cultured the following samples in each instance: preservation fluid (immediately before and at the end of the back-table procedure, a ... | 2016 | 27513941 |
| high-density lipoprotein binds to mycobacterium avium and affects the infection of thp-1 macrophages. | high-density lipoprotein (hdl) is involved in innate immunity toward various infectious diseases. concerning bacteria, hdl is known to bind to lipopolysaccharide (lps) and to neutralize its physiological activity. on the other hand, cholesterol is known to play an important role in mycobacterial entry into host cells and in survival in the intracellular environment. however, the pathogenicity of mycobacterium avium (m. avium) infection, which tends to increase worldwide, remains poorly studied. ... | 2016 | 27516907 |
| a new rapid method for direct antimicrobial susceptibility testing of bacteria from positive blood cultures. | rapid identification and antimicrobial susceptibility testing (ast) of the causative agent(s) of bloodstream infections can lead to prompt appropriate antimicrobial therapy. to shorten species identification, in this study bacteria were recovered from monomicrobial blood cultures by serum separator tubes and spotted onto the target plate for direct maldi-tof ms identification. proper antibiotics were selected for direct ast based on species identification. in order to obtain rapid ast results, b ... | 2016 | 27520338 |
| australian group on antimicrobial resistance australian enterobacteriaceae sepsis outcome programme annual report, 2014. | the australian group on antimicrobial resistance performs regular period-prevalence studies to monitor changes in antimicrobial resistance in selected enteric gram-negative pathogens. the 2014 survey was the second year to focus on blood stream infections. during 2014, 5,798 enterobacteriaceae species isolates were tested using commercial automated methods (vitek 2, biomérieux; phoenix, bd) and results were analysed using the clinical and laboratory standards institute (clsi) and european commit ... | 2016 | 27522134 |
| antibiotic utilization patterns in patients with ventilator-associated pneumonia: a canadian context. | this retrospective cohort study describes the patterns of antibiotic use for the treatment of ventilator-associated pneumonia (vap) in the calgary zone of alberta health services. timing, appropriateness, and duration of antibiotics were evaluated in two hundred consecutive cases of vap derived from 4 adult intensive care units (icu). antibiotic therapy was initiated in less than 24 hours from vap diagnosis in 83% of cases. although most patients (89%) received empiric therapy that demonstrated ... | 2016 | 27525016 |