Publications

[characteristics of the pkmr plasmids found in shigella flexneri].	the clinical isolate of sh. flexneri 1b, resistant to 5 antibiotics and sulfonamides, has been studied by the method of conjugation and found to have a group of transfer-suppressed pkmr-plasmids: pkmr 204-1 (ap sm tc cm km su), pkmr 204-2 (sm km su), pkmr 204-5 (km su) and pkmr 204-7 (sm tc cm km su), whose molecular weight was 99, 71.2, 73.8 and 59.5 md respectively. the treatment of the plasmids with restriction endonuclease bamhi has revealed that plasmids pkmr 204-2 and pkmr 204-5 are defini ...	1982	6285647
characterization of virulence plasmids and plasmid-associated outer membrane proteins in shigella flexneri, shigella sonnei, and escherichia coli.	the 140-megadalton plasmids of shigella flexneri serotypes 1, 3, and 5, in addition to the 120-megadalton plasmid of shigella sonnei, are associated with virulence. the present study showed that a 140-megadalton plasmid is also associated with virulence in escherichia coli. when these plasmids were cleaved with ecori or bamhi restriction endonucleases, considerable homology was evident in plasmids from s. sonnei strains, whereas only a few common fragments were observed among the s. flexneri and ...	1983	6299962
pms76, a plasmid capable of amplification by treatment with chloramphenicol.	pms76 is a nonconjugative, 5.54-megadalton plasmid. this plasmid is present in escherichia coli k12 cells at about 20 copies per chromosome. in addition, the pms76 plasmid can be mobilized by conjugative plasmids and it shares a number of other properties with the amplifiable cole1 plasmid, including the ability to amplify copy number in the presence of chloramphenicol. however, pms76 is compatible with cole1-like replicons, pbr313, and with other multicopy plasmids, rsf1030 and pacyc184. also t ...	1983	6300947
entamoeba invadens and e. histolytica: separation and purification of precysts and cysts by centrifugation on discontinuous density gradients of percoll.	the different cell forms in the life cycle of entamoeba invadens (trophozoites, precysts, and cysts) were rapidly and quantitatively separated on density step gradients of polyvinylpyrolidone-coated colloidal silica particles (percoll). with this method, the gradual process of encystation by e. invadens trophozoites could be monitored. percoll gradients were also efficient in separating trophozoites of entamoeba histolytica and bacteria. after purification on percoll, trophozoites display no evi ...	1983	6303827
the polymicrobial origin of intestinal infections in homosexual men.	to determine the microbial cause and the clinical and pathologic correlates of anorectal and intestinal symptoms in homosexually active men, we performed comprehensive microbiologic studies, anoscopy, sigmoid-oscopy, and rectal biopsy in men examined in a clinic for sexually transmitted diseases. enteric pathogens were found in 95 of 119 consecutive homosexual men with anorectal or intestinal symptoms and in 29 of 75 randomly selected homosexual men without such symptoms (p less than 0.001). the ...	1983	6308444
[fiessinger-leroy-reiter's syndrome : serological conversion for yersinia enterocolitica type 9, positive serodiagnosis for shigella flexneri and chlamydia].	a case of reiter disease in a hla b27 positive man with urethritis followed by polyarthritis is reported. during the disease, serologic conversion for yersinia enterocolitica type 9 was detected as well as positive serologic tests for shigella flexneri and chlamydia. the features of reactive arthritis related to yersinia and the etiologies of reiter disease are recalled.	1983	6314526
entamoeba histolytica: virulence enhancement of isoenzyme-stable parasites.	pathogenic entamoeba histolytica isolated from patients with clinical amoebiasis can be differentiated from nonpathogenic e. histolytica obtained from asymptomatic carriers on the basis of the electrophoretic pattern of their isoenzymes. virulence of different strains of axenically grown trophozoites of entamoeba histolytica, as determined by various laboratory tests, such as damage to tissue culture monolayers, or their ability to cause an hepatic abscess in a hamster, are known to vary conside ...	1984	6325226
comparative efficacies of pivmecillinam and ampicillin in acute shigellosis.	the clinical efficacies of pivmecillinam and ampicillin were compared in a randomized double-blind trial in the treatment of acute shigellosis. of 44 adult male patients, all culture positive for shigella strains, 22 patients received 400 mg of pivmecillinam and 22 patients received 500 mg of ampicillin every 6 h. both drugs were administered orally for 5 days. four patients receiving ampicillin were infected with shigella strains that were resistant to ampicillin but susceptible to pivmecillina ...	1984	6329092
plasmid-determined antibiotic resistance in shigella flexneri isolated in england and wales between 1974 and 1978.	the majority of shigella flexneri strains isolated in england and wales are from infections contracted abroad. most of these strains are drug-resistant, over 75% being resistant to streptomycin and sulphonamides, sulphonamides alone or streptomycin, sulphonamides and tetracyclines. a selection of resistant strains was tested for resistance transfer and the plasmids identified were characterized by compatibility grouping. streptomycin and sulphonamide resistance was usually determined by a non-au ...	1983	6337209
role of heat labile antigens of shigella flexneri in hela cell invasion.	in studies of the role of surface antigens of shigella flexneri in hela cell invasion, three antisera were employed to block the invasion. antisera against live (als) and boiled (abs) s. flexneri blocked invasion very effectively. reduction in the numbers of intracellular shigellae was always accompanied by reduction in the number adherent to the cells, indicating the importance of adhesion in the invasive process. anti-live absorbed antiserum (alas) prepared by exhaustive absorption of als with ...	1983	6337262
an evaluation of ocular inflammation following the injection of bacterial endotoxin into the rat foot pad.		1983	6337969
rapid method for detection, identification, and susceptibility testing of enteric pathogens.	three hundred and seven colonies believed to be enteric pathogens were selected from primary plates of macconkey, xylose desoxycholate, or salmonella-shigella agar for inoculation to lactose-sucrose broth, urea-41 motility medium, modified andrade glucose broth with inverted durham tube, pregrowth broth, triple sugar iron agar, lysine iron agar (lia), and christensen urea agar. the rapid screen consisted of interpreting the lactose-sucrose, urea-41 motility, and modified andrade glucose broth ga ...	1983	6338037
use of uv-irradiated bacteriophage t6 to kill extracellular bacteria in tissue culture infectivity assays.	we have utilized 'lysis from without' mediated by uv-inactivated bacteriophage t6 to eliminate extracellular bacteria in experiments measuring the internalization, intracellular survival and replication of yersinia pestis within mouse peritoneal macrophages and of shigella flexneri within a human intestinal epithelial cell line. the technique we describe has the following characteristics: (a) bacterial killing is complete within 15 min at 37 degrees c, with a greater than 10(3)-fold reduction in ...	1983	6338113
fulminating encephalopathy associated with shigella flexneri infection.	three cases of rapidly fatal encephalopathy associated with shigella flexneri infection are reported. there was a lack of severe intestinal involvement and absence of metabolic derangement. in all 3 patients, areas of necrosis were present throughout the brains; and in one case pontine haemorrhages and demyelination were seen. this report supports the evidence of a particular neurotoxic effect in shigellosis.	1983	6338835
[perforated duodenal ulcer in acute dysentery in a 7-year-old child].		1983	6340055
shigellosis with bacteremia: a report of two cases and a review of the literature.	it is widely believed by both physicians and microbiologists that bacteremia is a rare event in shigellosis. we report two cases of shigellosis with positive blood cultures and review 68 cases reported in the literature between 1963 and 1981. we suggest that detection of bacteremia in shigellosis may be facilitated by a greater awareness of the following findings. 1. eighty-seven percent of all cases of shigellosis with bacteremia reported in the literature during the 18-year period reviewed occ ...	1983	6340078
[effect of a live enteral dysentery vaccine from spontaneous flexner mutant 2a 516m on the immunoglobulin-producing cell count in the large intestine mucosa in dysentery].	the influence of vaccinal therapy with live oral dysentery vaccine prepared from s. flexneri 2a 516m on the content of immunoglobulin-producing cells in the mucous membrane of the large intestine was studied. a considerable increase in the number of iga- and igm-synthetizing cells was shown to occur in the course of the infectious process in acute dysentery. in chronic dysentery the content of iga- and igm-synthetizing cells in patients was considerably lower than in a smooth course of acute dys ...	1983	6340380
[specificity of the antibody neutralization reaction in indicating shigella antigens].	the specificity and sensitivity of the antibody neutralization test in the diagnosis of dysentery have been studied. this test has proved to be highly specific in experimental and clinical trials. the antibody neutralization test is more effective than the methods of the isolation of shigellae. the accuracy of this test can be increased by the elimination of different antierythrocyte agents from the secretions of persons under examination.	1983	6340381
[electron microscopic study of microorganisms grown on nutrient media with a nonnutrient raw material base].	a comparative electron-microscopic study of sh. flexneri 8512, str. dick ia and cor. pseudodiphthericum test strains, grown in experimental culture media prepared from inedible raw materials and in currently used culture media based on fish and meat, has been carried out. the ultrastructure of the test strains grown in culture media prepared from inedible raw materials has been shown to remain unchanged.	1983	6340385
[morphological characteristics of phagolysis of s- and r-forms of shigella flexneri].	the differences in the character and time of the lysis of s. flexneri s- and r-mutant cells have been detected by the method of time-lapse microfilming. the asymmetry of the phagolysis of the s-form of shigellae infected with phage f1 has been revealed.	1983	6340391
alterations in the pathogenicity of escherichia coli k-12 after transfer of plasmid and chromosomal genes from shigella flexneri.	a 140-megadalton plasmid (pwr110), which has previously been associated with virulence in shigella flexneri, was transferred to escherichia coli k-12. segments of s. flexneri chromosomal material were then transferred to the plamid-bearing k-12 strains. the virulence of these transconjugant hybrids was assessed in the hela cell model, in ligated rabbit ileal loops, or in the sereny test. a k-12 strain which harbored only pwr110 invaded hela cells, produced minimal lesions in the rabbit ileal muc ...	1983	6341237
shigella infections in the united states, 1974-1980.	during the seven-year period 1974-1980, 93,516 shigella isolates from humans were reported to the centers for disease control, atlanta, through a nationwide surveillance system. over the past 30 years, the reported incidence of shigellosis has been declining in contrast to that reported for salmonellosis. shigella sonnei (group d) now accounts for approximately 70% of the shigella isolates reported, since most of the decline has been due to the increasingly less frequent infections caused by shi ...	1983	6341480
[clinical characteristics of dysentery caused by shigella flexneri in middle-aged and elderly patients].		1983	6341707
[leukocyte migration activity in patients with dysentery].		1983	6342261
[epidemiology of bacillary dysentery in algeria. i. the epidemiological aspects of dysentery in algeria].	bacterial dysentery plays an important role among infectious diseases in algeria. a tendency to the growth of the morbidity rate has been observed. dysentery is irregularly spread in different zones of the country. the highest morbidity rate is registered in the sahara zone and in the eastern part of the country. the morbidity rate among the urban population is higher than among the rural population. s. flexneri prevail in the etiological structure of dysentery infection. of all age groups, the ...	1983	6342313
multiple enteric pathogens in travelers from endemic areas: case report.		1983	6344046
shigella septicaemia.		1983	6344366
delayed hypersensitivity in murine salmonellosis: specificity of footpad reaction in mice infected with rough mutants of salmonella typhimurium.	delayed type (footpad) hypersensitivity (dth) in balb/c mice immunized with rough mutant strains of salmonella typhimurium lt2 was examined. injection of live organisms of an rb mutant tv148 strain induced dth in mice, while injection of the heat-killed organisms did not. the mice immunized with live organisms of the ra, rb, rc, rd, and re mutant strains showed positive footpad reactions to the heat-killed cell antigen of lt2 (wild type) strain. the mice immunized with the rb mutant strain also ...	1983	6346026
[new directions in the study of immunity in dysentery].		1983	6346759
local iga-memory response to bacterial antigens.		1983	6347004
fulminating encephalopathy and shigella flexneri infection.		1983	6347094
gain of ornithine decarboxylase activity by a strain of shigella flexneri serotype 6 on the introduction of an r plasmid.	the introduction of r plasmid s-a into a strain of shigella flexneri serotype 6 resulted in the strain gaining the ability to decarboxylate ornithine. the possible reasons for the phenomenon are discussed and the result is compared with that obtained previously when the introduction of r plasmids into a different strain of the same species resulted in enhanced gas production from a range of carbohydrates.	1983	6348292
[joint use of antibiotics and antibiotic-resistant bifidobacteria in treating acute intestinal infections].	acute dysentery and other acute intestinal diseases with obscure etiology are accompanied by pronounced intestinal dysbacteriosis which is manifested, on one hand, by the increased levels of escherichia, enterobacteria, staphylococci and by the appearance of klebsiella and proteus in large amounts and, on the other hand, by an essential decrease in the number of lactic acid streptococci, lactobacteria, bifidobacteria. the treatment of dysentery and other intestinal diseases with obscure etiology ...	1983	6349186
[causes of sequential changes in the etiological structure of dysentery and the other epidemiologic patterns of this infection].		1983	6349187
synthesis of plasmid-coded heat-labile enterotoxin in wild-type and hypertoxinogenic strains of escherichia coli and in other genera of enterobacteriaceae.	the effect of host determinants on expression of plasmid-coded heat-labile enterotoxin (lt) was examined. a collection of lt plasmids was introduced into isogenic strains of escherichia coli k-12 strains containing the wild type or hypertoxinogenic (htx-2) allele. for each plasmid tested, production of lt increased by approximately 1.5- to 3-fold in the host containing htx-2, indicating that the htx-2 allele affects a regulatory function for lt production that is common to many different enterot ...	1983	6350177
the influence of bacterial exotoxins and endotoxins on the phagocytic activity of human macrophages in culture.	the effect of bacterial exotoxins and endotoxins on phagocytosis was tested on human macrophages in monolayer cultures by determining the rate of zymosan particle ingestion at different toxin concentrations and incubation times. the exotoxins tested were staphylococcal alpha-toxin and diphtheria-toxin. the endotoxins used were lipopolysaccharides from salmonella typhi, salmonella typhimurium, shigella flexneri and serratia marcescens. phagocytosis was significantly impaired after prolonged incub ...	1983	6350191
[importation of shigella and salmonella into east germany].	the international tourist traffic and the import of provisions also involves the importation of shigella and salmonella into the gdr. in 1980 9% of the shigella sonnei infections and 50% of the shigella flexneri infections were imported from 22 or 26 countries, in most cases from tourist countries, typical for the gdr. the salmonella are imported mainly by feed-stuff. but also the spreading particularly of polyresistant salmonella by epidemic processes independent of provisions, above all among ...	1983	6351449
[liquid-phase electrophoresis in the analysis of antigen-antibody interactions in erythrocyte diagnostic reagent kits].		1983	6351504
flies as a source of enteric pathogens in a rural village in thailand.	the village of ban pong in northeastern thailand was studied from january through december 1981 to determine the importance of flies as a source of enteric pathogens. the number of flies (predominantly musca domestica) increased in kitchens and animal pens in the hot dry spring, when the incidence of diarrhea was highest in the village. enterotoxigenic escherichia coli, shigella spp., non-o1 vibrio cholerae, and vibrio fluvalis were isolated from fly pools in yards (69%), animal pens (38%), bath ...	1983	6351748
effect of parenteral immunization on the local immunoglobulin a response of the intestine to shigella flexneri antigens.	most traditional methods of immunization involve parenteral vaccines. using a chronically isolated ileal loop model as the probe, we examined the effect of a primary parenteral immunization on the local immune response of the intestine. secretions from isolated ileal loops of rabbits given a primary parenteral immunization with shigella flexneri without adjuvant showed a small, but definite, specific immunoglobulin a (iga) response locally, whereas a vigorous serum antigen-specific igg response ...	1983	6352492
bacterial pathogens from the intestinal tracts of various species of cockroaches.		1983	6353184
fulminant hepatic failure and shigella bacteremia.		1983	6353185
[autonomic nervous system disorders in patients with sonne and flexner's dysentery].		1983	6353759
[current state of dysentery epidemiology (review of the literature)].		1983	6353765
[synthesis of beta-l-rhamnoside linked oligosaccharides of lipopolysaccharides from shigella flexneri serotype 6].	the synthesis of the trisaccharide o-beta-l-rhamnopyranosyl-(1 leads to 4)-o-beta-l-rhamnopyranosyl-(1 leads to 2)-l-rhamnopyranose (14) and the tetrasaccharide o-2-acetamido-2-deoxy-beta-d-galactopyranosyl)-(1 leads to 2)-o-[beta-l-rhamnopyranosyl-(1 leads to 4)]-o-beta-l-rhamnopyranosyl-(1 leads to 2)-l-rhamnopyranose (21) is described. the latter structure has been proposed as the repeating unit of the o-specific side-chain of the lipopolysaccharide obtained from shigella flexneri serotype 6. ...	1983	6354443
sexually transmitted enteritis.		1983	6355677
[biochemical aspects of the pathogenicity of shigella. protein components of aqueous extracts of virulent and non-virulent strains of shigella flexneri].		1983	6355709
invasive enteric pathogens.	invasive enteric pathogens of the salmonella or shigella genera initiate infections by invading the intestinal epithelium. depending on the species, salmonellae either translocate across the mucosa of the small intestine and cause a systemic febrile disease or they evoke a localized inflammatory response in discreet areas of the infected mucosa. the latter type of infection is characterized by gastroenteritis, and a choleragen-like enterotoxin may contribute to the symptomology. shigellae can al ...	1983	6356290
[trends in the spread of boyd 4 dysentery and the characteristics of its clinical picture and of the biology of its causative agent].	the data on the proportion of s. boydii 4 in the general structure of the causative agents of dysentery, as well as in the intraspecific structure of s. boydii, in some areas of the ussr in 1977-1981 are presented. s. boydii strains 4, circulating in one of the areas where their proportion considerably increased in 1980-1981, corresponded to their taxonomic position in their biological properties, while forming a single biochemical variant according to the character of the fermentation of sorbit ...	1983	6356722
[dynamics of enterobacterial multiplication in a mouse model of intraperitoneal infection in the presence of iron cations as dependent on the availability and type of k antigens].	in this work the results of research on the influence of iron cations on the characteristics of the infectious process caused by different enterobacteria (shigella sonnei, shigella flexneri, escherichia coli) are analyzed. in the experimental intraperitoneal infection of mice in the presence of saccharose and iron cations s. sonnei in phase i showed the decrease of 1g ld50 by 3-4 orders, while s. flexneri in the s-form, by not more than 1 order. the absence of correlation between the virulence o ...	1983	6356728
effect of human leukocyte interferon on invasiveness of salmonella species in hep-2 cell cultures.	the effect of human leukocyte interferon on the invasiveness of salmonella and shigella species in hep-2 cell cultures was examined. the intracellular and extracellular bacteria were identified by a combination of nomarski differential interference contrast microscopy and uv incident light microscopy applied on the same microscope. pretreatment of hep-2 cells with human leukocyte interferon reduced the number of salmonella typhimurium and salmonella paratyphi-b bacteria per cell and the proporti ...	1983	6358041
[differentiation of shigella flexneri serotype 6 from serotypes 1-5 by electrophoresis and isoelectric focusing of their elastases].	among shigella flexneri serotypes, serotype 6 (28 strains) was individualized from serotypes 1 to 5 (43 strains) by electrophoresis and isoelectric focusing of esterases. the taxonomic status of s. flexneri serotype 6 should be reconsidered in light of this work.	1983	6360018
temperature-dependent expression of virulence genes in shigella species.	the pathogenicity of shigella spp. involves the ability of the bacteria to penetrate and replicate within the epithelial cells of the large intestine. model systems for examining the virulence of shigellae employ henle intestinal epithelial cells in tissue culture and an in vivo assay for virulence in guinea pig eyes (sereny test). using these systems, we studied the genetic and physiological bases for the ability of shigellae to invade epithelial cells. we found that expression of virulence in ...	1984	6360895
loss of pigmentation in shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid.	in this study, we examined the relationship between the virulence of shigella flexneri 2a and the ability of strains of s. flexneri 2a to absorb congo red. spontaneous nonpigmented (i.e., unable to bind congo red [pcr-]) derivatives of a virulent, pigmented (pcr+) strain of s. flexneri 2a were isolated and assayed for virulence as determined by their ability to invade epithelial cells. all pcr- mutants examined lost the ability to invade epithelial cells and were thus avirulent. agarose gel elec ...	1984	6360906
splenic abscess caused by shigella flexneri and bacteroides fragilis.	we report a case of splenic abscess from which only shigella flexneri (serotype 1a) and bacteroides fragilis were isolated. the patient was a 59-year-old diabetic female who displayed minimal gastrointestinal symptoms. the s. flexneri isolate was resistant to chloramphenicol and tetracycline. the possible synergistic relationship between the two organisms is discussed.	1984	6361064
[clinico-laboratory characteristics of gastroenterocolitic (atypical) dysentery].		1983	6362054
change in phage-sensitivity of shigella flexneri strains. i. changes in virulence and phage-sensitivity.	in the course of repeated passaging of virulent shigella flexneri cultures in liquid media, avirulent variants and variants of reduced virulence appeared. they were easily recognizable by their colonial morphology. the avirulent variants became sensitive to certain phages not lysing the original virulent strains. the change of phage-sensitivity among the variants with no or decreased virulence was significant: chi 2 = 64.25; p less than 0.001. the amplification of phage-sensitivity developed in ...	1983	6362315
[dissociation and phagolytic characteristics of the shigella isolated from patients with various clinical forms of dysentery].	the relationship between the severity of the disease and the phagolysis of the isolated cultures of s. flexneri and s. sonnei has been established. in the moderately severe form of the disease the duration of the latent period of the development of the phage has been found to increase in comparison with that in the mild form of the disease.	1983	6364654
lipid a fractions analyzed by a technique involving thin-layer chromatography and enzyme-linked immunosorbent assay.	a modified enzyme-linked immunosorbent assay (elisa), with alkaline phosphatase as enzyme, was used for the study of antigenicity of lipid a fractions directly on thin-layer chromatographic (tlc) plates. for visualization a gel slab containing the enzyme substrate was placed on the plate containing enzyme-conjugated antibodies. the plate was read by a thin-layer chromatogram spectrophotometer. the immunoassay was both highly specific and quite sensitive. sensitivity was superior to levels obtain ...	1984	6365543
[food infection caused by shrimp].		1984	6366590
[chromosome markers of drug resistance as the selectors of potential vaccinal strains].	mutations resulting in acquisition of the resistance to rifampicin, streptomycin, neamine, polymyxin m, mecillinam and high concentrations of nalidixic acid were studied with respect to their effect on virulence of shigella flexneri by transduction of the markers from the spontaneous avirulent mutants to the initial strain. it was shown that the acquisition of the determinants of the resistance to rifampicin, streptomycin, neamine (nea a) and high concentration of nalidixic acid resulted in 100 ...	1984	6367629
[transfer of resistance in shigellae to azlocillin and other antibiotics].		1984	6367893
r plasmids conferring multiple drug resistance from shigella isolated in korea.	the majority (85%) of shigella isolated in 1980 and 1981 in korea were shigella flexneri, the others were sh. sonnei (14%) with only a small number of sh. dysenteriae. only 14 of the 459 strains of shigella isolated were susceptible to all 12 drugs tested, and 445 were resistant to three or more drugs. strains multiply resistant to the six drugs, chloramphenicol (cm), tetracycline (tc), streptomycin (sm), sulfisomidine (su), ampicillin (ap) and trimethoprim (tp) were most frequently encountered, ...	1984	6368684
[effect of plasmids r on the changes in s. flexneri sensitivity to phages].		1983	6369040
[clinico-immunological characteristics of dysentery].		1983	6369561
[further research on the use of media with na-rna for isolating shigella].	the isolation of shigellae from the feces of patients with the use of ploskirev's bactoagar with na-rna added at a concentration of 4 mg/ml has facilitated the laboratory diagnosis of dysentery due to the increased resolution of the medium. the isolation of shigellae on the medium with the stimulating agent added occurs 9% more frequently than on the control medium.	1984	6369838
shigella dysentery in tripoli, libya.		1984	6371260
leads from the mmwr. shigellosis among tourists--union of soviet socialist republics, 1983.		1984	6371268
[bacillary dysentery (shigella flexneri type 2) caused by shrimps].		1984	6371557
[transfer of h2s plasmid from e. coli to shigella].		1983	6374333
[basic approach to the production of potential vaccine strains of salmonella, shigella and pasteurella, capable of reproduction, using suitable genetic markers].		1984	6375171
[interrelation of virulent and avirulent (vaccinal) strains of shigella flexneri 2a in long-term bacterial carriage in gnotobiotic rats].	the ability of s. flexneri 2a virulent and avirulent (vaccine) strains no. 516 and no. 516 m to displace one another during long-term carrier state in germ-free rats has been studied. the long-term persistence of the vaccine strain in the intestine of the rats has been shown to produce colonization resistance to subsequent infection with the virulent culture of these bacteria. during carrier state in rats progressive s--r dissociation of the bacteria occurs, type ii antigen is lost with simultan ...	1984	6375221
sulfamethoxazole-trimethoprim-resistant shigella flexneri in northeastern brazil.	in contrast to prior experience in northeastern brazil, three of four shigella flexneri strains recently isolated from patients with acute inflammatory diarrhea in this setting were found to be resistant to sulfamethoxazole-trimethoprim. the resistant strains contained large, different plasmids, two of which were transferred with sulfamethoxazole-trimethoprim resistance to escherichia coli k-12 recipient strains.	1984	6375563
[relation of the epidemiological characteristics of dysentery to the biological properties of the causative agents].	the main forms of the epidemic manifestation of dysentery induced by different causative agents in 1975-1980 were revealed. during these years the cases of dysentery induced by shigella sonnei, biovar ii, were found to prevail (82.0-90.1%) both at the periods between epidemics and at the periods of the seasonal rise of morbidity. the experimental infection of white mice by intraperitoneal inoculation revealed no relationship between the seasonal rise of morbidity in dysentery and the virulence o ...	1984	6377769
identification of hla-b27m1 and -m2 cross-reactive antigens in klebsiella, shigella and yersinia.		1984	6378768
[arthritis in shigella and salmonella infections].		1984	6379799
an outbreak of shigellosis in an ultra-orthodox jewish community.	an outbreak of shigellosis due to shigella sonnei, s. flexnerii and s. boydii in an ultra-religious jewish community in southern israel is described. the source of epidemic was traced to vegetables bought from a single source. the importance of epidemic and endemic modes of spread of shigella is discussed.	1984	6379888
[clinical characteristics of dysentery in relation to the oxidation-reduction features of isolated shigella].		1984	6379895
[shigellosis abroad].		1983	6379992
changes in the trend of shigellosis in dhaka: family study on secondary infection, clinical manifestation and sensitivity pattern: 1980.	the incidence of shigellosis and the death rate have increased and the resistance of shigellae to antibiotics has changed in dhaka during our experiences. in 1980, we investigated the secondary infection and case rates, infection to case ratio, duration of illness, excretion of shigellae and antibiotic sensitivity pattern in 100 families with cases of shigellosis, culturing rectal swabs obtained by home visits for a 10-day period. standard methods were used for culture and sensitivity tests. the ...	1984	6380011
[structure of a shigella flexneri population in the process of forming microcolonies].	the study of the adaptation period of the s- and r-forms of s. flexneri in a nutritionally sufficient medium has revealed that the population structure characteristic of the s-form and ra-chemotype comprises two classes of cells: actively growing and nonviable. besides these two classes, the class of spontaneously lysing bacteria is present in the population of mutants with various degrees of lipopolysaccharide (lps) lesions. the number of bacteria subject to autolysis increases with the progres ...	1984	6380164
purification and properties of carboxylesterase b of escherichia coli.	carboxylesterase b produced by escherichia coli was purified 1,350-fold with a recovery of 12% by successive gel filtrations, deae-trisacryl, phenyl-sepharose chromatography and preparative electrophoresis. the purified enzyme was found to be homogeneous, as judged by a single precipitation line in ouchterlony double diffusion in an experiment with homologous antiserum. the apparent molecular weight determined by gel filtration and the isoelectric point determined by electrofocusing were 57,000 ...	1984	6380369
cytotoxicity of human peripheral blood and colostral leukocytes against shigella species.	we examined the ability of human peripheral blood leukocytes to kill strains of shigella sonnei and shigella flexneri by using a modified bactericidal assay. antibody-dependent cellular cytotoxicity (adcc) was demonstrated in the presence of specific rabbit immune serum directed against s. sonnei. with peripheral blood leukocytes from adults, adcc was found only in the mononuclear cell and purified lymphocyte populations. monocyte-macrophages and polymorphonuclear leukocytes were unable to demon ...	1984	6384045
heterogeneity of lipid a: comparison of lipid a types from different gram-negative bacteria.	chloroform-soluble purified lipid a preparations from 10 sources, including five escherichia coli strains (eh100, k-12, o127, o111, rcdc), two salmonella strains (salmonella typhimurium, salmonella minnesota r595), shigella sonnei ii, and a hybrid of shigella flexneri and e. coli k-12, were compared with lipid a from s. flexneri. purified lipid a from s. flexneri was earlier found to be composed of eight fractions. the various lipid a preparations were assayed by thin-layer chromatography. chrom ...	1984	6384185
effect of cytochalasin b and dihydrocytochalasin b on invasiveness of entero-invasive bacteria in hep-2 cell cultures.	the effect of cytochalasin b (cb) and di-hydrocytochalasin b (h2cb) on the invasiveness of salmonella typhimurium, shigella flexneri and yersinia enterocolotica serotype 0:3 in hep-2 cell cultures was examined. the intra-cellular and extra-cellular bacteria were identified by a combination of nomarski interference contrast microscopy and uv incident light microscopy applied on the same light microscope. pre-treatment of cells with cb and h2cb inhibited the uptake of s. typhimurium and s. flexner ...	1984	6385614
usefulness of plasmid profiles for differentiation of shigella isolates in bangladesh.	we studied the plasmid profiles of 136 shigella isolates in bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. many different plasmid patterns were observed within each species, indicating that many genetically different strains of shigella are responsible for illness in bangladesh.	1984	6386850
oral vaccination of monkeys with an invasive escherichia coli k-12 hybrid expressing shigella flexneri 2a somatic antigen.	a living oral vaccine, designed to protect against shigella flexneri 2a infections, was constructed by using escherichia coli k-12 as a carrier strain. the hybrid strain, designated ec104, contained both chromosomal and plasmid genes from s. flexneri donor strains. in addition to expressing the s. flexneri 2a somatic antigen, it had inherited the property of epithelial-cell invasion. after the oral administration to rhesus monkeys, ec104 was isolated from the feces for up to 3 days, but by day 4 ...	1984	6389344
the prevalence of gastro-intestinal parasites and bacteria in the game scouts at the kainji lake national park of nigeria.	the faeces of twenty scouts working at borgu games were examined for the presence of parasites and bacteria. cysts of entamoeba histolytica, giardia lamblia and trichomonas hominis were found. helminth ova encountered included those of ancylostoma duodenale, ascaris lumbricoides, stronglyloides stercoralis and trichuris trichiura. escherichia coli and shigella flexneri were isolated from one faecal sample each. in all, parasites were found in the faeces of only 9 scouts. the epidemiological sign ...	1984	6389403
[bacillary dysentery 1982].		1984	6390523
[clinical and treatment characteristics of acute dysentery caused by shigella boydii and newcastle].		1984	6390958
[further decrease in intestinal infection morbidity in the ukrainian ssr].		1984	6390962
[conditions and factors determining the etiological structure of dysentery and its alterations. ii. concepts, hypotheses and points of view yesterday and today].		1984	6391040
[conditions and factors determining the etiological structure of dysentery and its alterations. iii. the epidemiological assessment of the biological properties of shigella and their importance in the development of the epidemic process in different nosological forms of dysentery].		1984	6391041
dna hybridization technique to detect shigella species and enteroinvasive escherichia coli.	a dna hybridization method for the detection of shigella species and enteroinvasive escherichia coli is described. it is based upon the high degree of homology shared by the virulence plasmids present in all pathogenic strains. after 32p labeling, a 17-kilobase ecori restriction fragment of a virulence plasmid belonging to shigella flexneri serotype 5 was used as a probe to detect shigella species and enteroinvasive e. coli in isolated colonies. this method proved highly specific as well as sens ...	1984	6392327
shigella vulvovaginitis in a three-year-old child: a case report.		1984	6392838
[identification of specific antibodies in dysentery].		1984	6393359
shigellemia.		1984	6394497
association of pneumonia with undernutrition and shigellosis.		1984	6394498
the effect of postnatal age on the adherence of shigella flexneri, escherichia coli 0124, and e. coli 0128 to guinea pig intestinal cells.	since postnatal development of the gastrointestinal tract has an important effect on its microbial flora and may influence the types of intestinal infections, we examined the effect of age on bacterial adherence to intestinal epithelial cells. radiolabeled bacteria were incubated with guinea pig enterocytes released by treating loops of the intestine with solutions containing edta, dithiothreitol, and citrate. nonbound bacteria were separated from intestinal cells by sedimentation on a percoll g ...	1984	6395082
[species of shigella isolated in a 4-year period and antimicrobial sensitivity].		1984	6395307