Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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effects of water potential on spore germination and viability of fusarium species. | germination of macroconidia and/or microconidia of 24 strains of fusarium solani, f. chlamydosporum, f. culmorum, f. equiseti, f. verticillioides, f. sambucinum, f. oxysporum and f. proliferatum isolated from fluvial channels and sea beds of the south-eastern coast of spain, and three control strains (f. oxysporum isolated from affected cultures) was studied in distilled water in response to a range of water potentials adjusted with nacl. (0, -13.79, -41.79, -70.37, -99.56 and -144.54 bars). the ... | 2008 | 18712540 |
the interactive effects of temperature and osmotic potential on the growth of marine isolates of fusarium solani. | the mycelial growth of 18 fusarium solani strains isolated from sea beds of the south-eastern coast of spain was tested on potato-dextrose-agar adjusted to different osmotic potentials with either kcl or nacl (-1.50 to -144.54 bars) in 10 degrees c intervals ranging from 15 to 35 degrees c. fungal growth was determined by measuring colony diameter after 4 days of incubation. mycelial growth was maximal at 25 degrees c. the quantity and frequency pattern of mycelial growth of f. solani differ sig ... | 2008 | 18712541 |
loop grafting of bacillus subtilis lipase a: inversion of enantioselectivity. | lipases are successfully applied in enantioselective biocatalysis. most lipases contain a lid domain controlling access to the active site, but bacillus subtilis lipase a (lipa) is a notable exception: its active site is solvent exposed. to improve the enantioselectivity of lipa in the kinetic resolution of 1,2-o-isopropylidene-sn-glycerol (ipg) esters, we replaced a loop near the active-site entrance by longer loops originating from fusarium solani cutinase and penicillium purpurogenum acetylxy ... | 2008 | 18721749 |
lignin degradation in wood-feeding insects. | the aromatic polymer lignin protects plants from most forms of microbial attack. despite the fact that a significant fraction of all lignocellulose degraded passes through arthropod guts, the fate of lignin in these systems is not known. using tetramethylammonium hydroxide thermochemolysis, we show lignin degradation by two insect species, the asian longhorned beetle (anoplophora glabripennis) and the pacific dampwood termite (zootermopsis angusticollis). in both the beetle and termite, signific ... | 2008 | 18725643 |
phenomenological model of fungal biofilters for the abatement of hydrophobic vocs. | this work describes the growth of filamentous fungi in biofilters for the degradation of hydrophobic vocs. the study system was n-hexane and fusarium solani b1. the system is mathematically described and the main physical, kinetic data and morphological parameters were obtained by independent experiments and validated with data from laboratory experiments. the model describes the increase in the transport area by the growth of the filamentous cylindrical mycelia and its relation with n-hexane el ... | 2008 | 18781682 |
a thermostable chitinase with chitin-binding activity from phaseolus limensis. | a 28.6-kda chitinase with chitin-binding activity was isolated from the large lima bean (phaseolus limensis) seeds. the procedure entailed extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, and high-performance liquid chromatography (hplc) on sp-toyopearl. there was an almost 108-fold increase in specific activity of the purified chitinase compared with that of the crude extract. the enzyme exhibited a pi of 7.8 by isoelectric focusing electrophoresis. the ... | 2008 | 18782228 |
isolation, characterization and cloning of a cdna encoding a new antifungal defensin from phaseolus vulgaris l. seeds. | the pvd1 defensin was purified from phaseolus vulgaris (cv. pérola) seeds, basically as described by terras et al. [terras frg, schoofs hme, de bolle mfc, van leuven f, ress sb, vanderleyden j, cammue bpa, broekaer twf. analysis of two novel classes of plant antifungal proteins from radish (raphanus sativus l.) seeds. j biol chem 1992;267(22):15301-9], with some modifications. a deae-sepharose, equilibrated with 20mm tris-hcl, ph 8.0, was initially utilized for the separation of peptides after a ... | 2008 | 18786582 |
fungal endophthalmitis: fourteen years' experience from a centre in india. | background:: as fungal endophthalmitis is an emerging challenge, the study was carried out to determine the prevalence and the spectrum of fungal agents causing endophthalmitis from a single center, to identify the risk factors, and to correlate clinical course of illness with the agents involved. methods:: the microbiological and clinical records of all fungal endophthalmitis diagnosed during january 1992 through december 2005 at a tertiary center in india were reviewed retrospectively. during ... | 2008 | 18791488 |
fusarium species detected in onychomycosis in colombia. | fusarium spp. have frequently been isolated from patients with onychomycosis. in colombia, several studies have shown that fusarium is the most common non-dermatophyte mould causing onychomycosis and its spread has increased in the past years. in this study, samples were collected in 2003 and 2004 from 137 patients who were diagnosed with onychomycosis caused by fusarium spp. three species of fusarium were identified: fusarium solani (64.9%), fusarium oxysporum (32.8%) and fusarium verticillioid ... | 2008 | 18793261 |
[disseminated fusariosis in a patient with acute lymphoblastic leukaemia]. | invasive mould infections are a major cause of infectious mortality in highly immunosuppressed patients. incidence in this high risk group is 10-20% with a death rate in excess of 50%. most invasive moulds are aspergillus spp. we present a case of a 74-year-old woman with acute lymphoblastic leukaemia who developed a rare disseminated mould infection with fusarium solani during induction chemotherapy. we present the case story and discuss the pathogenesis, clinical characteristics and treatment ... | 2008 | 18796288 |
metal based isatin-derived sulfonamides: their synthesis, characterization, coordination behavior and biological activity. | some isatin derived sulfonamides and their transition metal [co(ii), cu(ii), ni(ii), zn(ii)] complexes have been synthesized and characterized. the structure of synthesized compounds and their nature of bonding have been inferred on the basis of their physical (magnetic susceptibility and conductivity measurements), analytical (elemental analyses) and spectral (ir, (1)h nmr and (13)c nmr) properties. an octahedral geometry has been suggested for co(ii), ni(ii) and zn(ii) and square-planar for cu ... | 2009 | 18825557 |
in silico comparison of transcript abundances during arabidopsis thaliana and glycine max resistance to fusarium virguliforme. | sudden death syndrome (sds) of soybean (glycine max l. merr.) is an economically important disease, caused by the semi-biotrophic fungus fusarium solani f. sp. glycines, recently renamed fusarium virguliforme (fv). due to the complexity and length of the soybean-fusarium interaction, the molecular mechanisms underlying plant resistance and susceptibility to the pathogen are not fully understood. f. virguliforme has a very wide host range for the ability to cause root rot and a very narrow host r ... | 2008 | 18831797 |
isolation of autochthonous non-white rot fungi with potential for enzymatic upgrading of venezuelan extra-heavy crude oil. | the increasing world demand for fuels makes it necessary to exploit the largest reserve of extra-heavy crude oil (ehco) of the orinoco oil belt from venezuela. we propose the use of extracellular oxidative enzymes, in particular, lignin-degrading enzyme systems (lds) of fungi, for enzymatic improvement of ehco. autochthonous non-white rot fungal strains able to use ehco, and several polycyclic aromatic hydrocarbons (pahs) as sole carbon source and energy, were isolated from ehco-polluted soils a ... | 2007 | 18833334 |
isolation and biochemical characterization of a novel leguminous defense peptide with antifungal and antiproliferative potency. | leguminous plants have formed a popular subject of research owing to the abundance of proteins and peptides with important biological activities that they produce. the antifungal proteins and peptides have been purified from a number of leguminous species. however, research continues to discover novel antifungal plant-produced peptides and proteins are being needed, specially those novel ones with both antifungal activity and other significant bioactivities. the objective of this study was to is ... | 2009 | 18841359 |
on the mechanism of enzyme action; fat formation in fusaria in the presence of a pigment obtained from fusarium solani d2 purple. | 1948 | 18860062 | |
quantification of fusarium solani f. sp. phaseoli in mycorrhizal bean plants and surrounding mycorrhizosphere soil using real-time polymerase chain reaction and direct isolations on selective media. | abstract the capacity of the arbuscular mycorrhizal fungus glomus intraradices in reducing the presence of fusarium solani f. sp. phaseoli in bean plants and the surrounding mycorrhizosphere soil was evaluated in a compartmentalized experimental system. quantification of the pathogen and the symbiont in plant tissues, the soil regions of the mycorrhizosphere (rhizosphere and mycosphere), and the bulk soil was accomplished using specific polymerase chain reaction (pcr) primers in real-time pcr as ... | 2003 | 18943138 |
interaction of fusarium solani f. sp. glycines and heterodera glycines in sudden death syndrome of soybean. | abstract sudden death syndrome (sds) of soybean is caused by the soilborne fusarium solani f. sp. glycines (synonym f. virguliforme). in a sequential approach, two multifactor factorial-design microplot experiments were conducted to investigate the effects of fungal infestation levels and soil moisture on both root necrosis and foliar sds severity, and the interaction between f. solani f. sp. glycines and heterodera glycines in fumigated versus nonfumigated soil. in 2003, soybean cv. spencer was ... | 2006 | 18943150 |
a promoter from pea gene drr206 is suitable to regulate an elicitor-coding gene and develop disease resistance. | abstract plant nonhost disease resistance is characterized by the induction of multiple defense genes. the pea drr206 gene is induced following inoculation with pathogens and treatment with abiotic agents, and moderately induced by wounding. a deletion series of drr206 promoter segments was fused with the beta-glucuronidase (gus) reporter gene and transiently transferred to tobacco, potato, and pea. gus activity revealed that two upstream regions of the drr206 promoter were particularly importan ... | 2004 | 18943490 |
interactions between the soybean cyst nematode and fusarium solani f. sp. glycines based on greenhouse factorial experiments. | abstract the soybean cyst nematode, heterodera glycines, and the fungus that causes sudden death syndrome (sds) of soybean, fusarium solani f. sp. glycines, frequently co-infest soybean (glycine max) fields. the interactions between h. glycines and f. solani f. sp. glycines were investigated in factorial greenhouse experiments with different inoculum levels of both organisms on a soybean cultivar susceptible to both pathogens. measured responses included root and shoot dry weights, h. glycines r ... | 2006 | 18943675 |
pea-fusarium solani interactions contributions of a system toward understanding disease resistance. | this mini-review points to the usefulness of the pea-fusarium solani interaction in researching the biochemical and molecular aspects of the nonhost resistance components of peas. this interaction has been researched to evaluate the resistance roles of the phytoalexin, pisatin, the cuticle barrier, and the activation of the nonhost resistance response. concurrently, evaluations of associated signaling processes and the tools possessed by the pathogen to contend with host obstacles were included. ... | 2008 | 18944184 |
breeding for highly fertile isolates of nectria haematococca mpvi that are highly virulent on pea and in planta selection for virulent recombinants. | abstract the heterothallic ascomycete nectria haematococca mating population vi (anamorph fusarium solani) is a broad host range pathogen. field isolates of this fungus that are pathogenic on pea tend to be female sterile, of low fertility, and the same mating type (mat-1), whereas female fertile isolates of either mating type that are highly fertile tend to be nonpathogenic on this plant. to facilitate genetic analysis of traits that may be important in the ability of n. haematococca to parasit ... | 2001 | 18944283 |
molecular differentiation of fusarium solani f. sp. glycines from other f. solani based on mitochondrial small subunit rdna sequences. | fusarium solani is a soilborne plant pathogen that infects many different hosts. within the species, there is some specialization, and a number of forma specialis have been described based on host affiliation. one of these, f. solani f. sp. glycines, infects soybean and causes sudden death syndrome. to differentiate between f. solani f. sp. glycines and other f. solani isolates, a partial sequence of the mitochondrial small subunit (mtssu) rrna gene was amplified by polymerase chain reaction and ... | 2000 | 18944555 |
effects of herbicides on fusarium solani f. sp. glycines and development of sudden death syndrome in glyphosate-tolerant soybean. | abstract sudden death syndrome of soybean, caused by fusarium solani f. sp. glycines, is a disease of increasing economic importance in the united states. although the ecology of sudden death syndrome has been extensively studied in relation to crop management practices such as tillage, irrigation, and cultivar selection, there is no information on the effects of herbicides on this disease. three herbicides (lactofen, glyphosate, and imazethapyr) commonly used in soybean were evaluated for their ... | 2000 | 18944572 |
suppression of fusarium solani f. sp. phaseoli on bean by aluminum in acid soils. | abstract the severity of bean root rot caused by fusarium solani f. sp. phaseoli in vitro was studied with regard to exchangeable soil aluminum for 25 soil samples collected from northeastern honshyu island, japan. of these, 24 were andosols, typically acidic and of volcanic ash origin. disease severity was assessed based on the number of lesions produced by the pathogen on a 6-cm section of bean stem buried and incubated for 8 days at 25 degrees c in artificially infested soil samples. the numb ... | 1999 | 18944802 |
carbon loss and germinability, viability, and virulence of chlamydospores of fusarium solani f. sp. phaseoli after exposure to soil at different ph levels, temperatures, and matric potentials. | abstract (14)c-labeled chlamydospores of fusarium solani f. sp. phaseoli were exposed to soil at 5, 15, 25, or 30 degrees c at ph 5 or 8 and water potential of -1 kpa or to soil at 0, -1, or -10 kpa at 25 degrees c at ph 6.9. total carbon loss was greatest at 25 or 30 degrees c at ph 8 and -1 kpa. (14)co(2) from respiration of chlamydospores and from soil microbes utilizing chlamydospore exudates accounted for the largest share of total carbon loss under all conditions. (14)(co)(2) from soil mic ... | 1998 | 18944984 |
comparison of soil receptivity to thielaviopsis basicola, aphanomyces euteiches, and fusarium solani f. sp. pisi causing root rot in pea. | abstract soil receptivity as a quantifiable characteristic ranging from conduciveness to suppressiveness to soilborne pea pathogens thielaviopsis basicola and aphanomyces euteiches was determined by analysis of differences in disease response curves obtained by artificial introduction of inoculum into natural field soil samples. several parameters, including maximum root rot severity, the area under the health index curve, scores on the first axis of a principal component analysis (pca) on dose ... | 1997 | 18945109 |
[effect of organic amendments on soil fungi community and continuous cropping panax quinquefolium]. | to study the effect of different organic amendments treated replant soil and to find the relations between the soil fungi community and the growth of panax quinquefolium l. seedling. | 2008 | 18973004 |
bacteria and free-living amoeba in the lascaux cave. | the lascaux cave was discovered in 1940, and by 1960 it had received up to 1800 daily visitors. in 1963, the cave was closed and in 2001 it was invaded by a fusarium solani species complex which was treated for four years with benzalkonium chloride. however, lascaux cave bacteria have only been poorly investigated. here we show that the cave is now a reservoir of potential pathogenic bacteria and protozoa which can be found in outbreaks linked to air-conditioning systems and cooling towers in co ... | 2009 | 18977432 |
catalysis by glomerella cingulata cutinase requires conformational cycling between the active and inactive states of its catalytic triad. | cutinase belongs to a group of enzymes that catalyze the hydrolysis of esters and triglycerides. structural studies on the enzyme from fusarium solani have revealed the presence of a classic catalytic triad that has been implicated in the enzyme's mechanism. we have solved the crystal structure of glomerella cingulata cutinase in the absence and in the presence of the inhibitors e600 (diethyl p-nitrophenyl phosphate) and petfp (3-phenethylthio-1,1,1-trifluoropropan-2-one) to resolutions between ... | 2009 | 18983850 |
thctf1 transcription factor of trichoderma harzianum is involved in 6-pentyl-2h-pyran-2-one production and antifungal activity. | we describe the cloning and characterization of the trichoderma harzianum thctf1 gene, which shows high sequence identity with a transcription factor gene of fusarium solani f. sp. pisi. in t. harzianum, disruption of the thctf1 gene by homologous recombination gave rise to transformants that in plate experiments did not show the yellow pigmentation observed in the wild-type strain. in several trichoderma spp. a yellow pigmentation and a coconut aroma have been related to the production of 6-pen ... | 2009 | 19007898 |
fungal endophthalmitis: fourteen years' experience from a center in india. | as fungal endophthalmitis is an emerging challenge, the study was carried out to determine the prevalence and the spectrum of fungal agents causing endophthalmitis from a single center, to identify the risk factors, and to correlate clinical course of illness with the agents involved. | 2008 | 19009680 |
genetic variability and relationships among seventeen trichoderma isolates to control dry root rot disease using rapd markers. | trichoderma spp. has been identified as potential antagonist of fusarium solani, which is causing dry root rot of citrus. a random amplified polymorphic dna (rapd) marker was used to estimate the genetic variation among 17 isolates of trichoderma. these isolates were characterized using 20 random primers of the opm series, out of which 16 primers gave a total of 145 dna fragments, showing 91.8% polymorphism. the genetic distance between each isolate was calculated, and cluster analysis was used ... | 2008 | 19040115 |
chemical composition and antifungal activity of volatiles from three opuntia species growing in tunisia. | the chemical composition of the volatiles isolated by steam distillation from leaves, flowers and fruits of opuntia lindheimeri var. linguiformis l. benson, leaves and flowers of opuntia macrorhiza engelm and leaves of opuntia microdasys (lehmann) gathered in the sea cliff of monastir town (tunisia), has been studied by gc and gc-ms. remarkable differences were noted between the composition and the constituent percentage of the different studied organs. the most important compounds found in leav ... | 2007 | 19070119 |
potential role of macrophages in experimental keratomycosis. | to investigate the potential role of macrophages in keratomycosis. | 2009 | 19074808 |
development of a nested pcr assay for the detection of fusarium solani dna and its evaluation in the diagnosis of invasive fusariosis using an experimental mouse model. | fusarium infections are increasingly being encountered in immunocompromised patients. fusarium solani accounts for nearly half of these infections. a specific nested pcr (npcr) assay has been developed by using dna isolated from several fusarium species and other common fungi. furthermore, dna samples isolated from bronchoalveolar lavage (bal) and serum samples from mice infected intravenously with f. solani conidia and sacrificed on every third day post infection were used for the evaluation of ... | 2010 | 19076280 |
overexpression of the chitosanase gene in fusarium solani via agrobacterium tumefaciens-mediated transformation. | to overexpress the chitosanase gene (csn) in f. solani, a vector based on pcambia 1300 was constructed. the csn gene, which is under control of the aspergillus nidulans gpda promoter and a. nidulans trpc terminator, was introduced back into the f. solani genome by agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from streptomyces hygroscopicus was used as the selection marker. transformants which showed a significant increase in chitosanase production (app ... | 2009 | 19093150 |
utility of molecular sequence analysis of the its rrna region for identification of fusarium spp. from ocular sources. | fungal ocular infections cause significant ocular morbidity, particularly when diagnosis and treatment are delayed. accurate morphologic identification of fusarium spp. beyond the genus is time-consuming and insensitive. it was the purpose of this study to examine the usefulness of the nuclear ribosomal rna (rrna) internal transcribed spacer regions (its1 and -2) to detect and differentiate fusarium spp. responsible for ocular infections. | 2009 | 19136697 |
isolation and characterisation of polychlorinated biphenyl (pcb) degrading fungi from a historically contaminated soil. | abstract: | 2009 | 19138404 |
[cloning of a homologous gene of magnaporthe grisea pmk1 type mapk from ustilaginoidea virens and functional identification by complement in magnaporthe grisea corresponding mutant]. | cloning of a homologous gene of pmk1 type mitogen-activated protein kinase (mapk) from the rice false smut fungus ustilaginoidea virens. | 2008 | 19149162 |
detection of (1-->3)-beta-d-glucan as an adjunct to diagnosis in a mixed population with uncommon proven invasive fungal diseases or with an unusual clinical presentation. | this single-center observational prospective study evaluated the performance of (1-->3)-beta-d-glucan as an adjunct diagnostic tool in 12 patients with proven invasive fungal disease with different risk factors. the infections were due to either uncommon fungal pathogens such as dematiaceous molds (scedosporium apiospermum, alternaria infectoria, and cladosporium macrocarpum) and hyaline septate molds (fusarium solani and blastoschizomyces capitatus) or aspergillus spp. with unusual clinical pre ... | 2009 | 19158282 |
effect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by fusarium solani in a rabbit model: a pilot study. | to evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental fusarium solani keratitis in rabbits. | 2008 | 19169521 |
synthesis and antimicrobial activity of 1-benzhydryl-sulfonyl-4-(3-(piperidin-4-yl) propyl)piperidine derivatives against pathogens of lycopersicon esculentum: a structure-activity evaluation study. | several 1-benzhydryl-sulfonyl-4-(3-(piperidin-4-yl)propyl)piperidine derivatives 8(a-j) were prepared by the treatment of substituted benzhydryl chlorides with 4-(3-(piperidin-4-yl)propyl)piperidine followed by n-sulfonation with sulfonyl chlorides in the presence of dry methylene dichloride and triethyl amine. the synthesized compounds were characterized by (1)h-nmr, ir, and elemental analysis. all the synthesized compounds were evaluated in vitro for their efficacy as antimicrobial agents by a ... | 2009 | 19183874 |
a comparison of regimen methods for the removal and inactivation of bacteria, fungi and acanthamoeba from two types of silicone hydrogel lenses. | to compare the antimicrobial efficacy of commercial contact lens solutions when used according to the manufacturers' recommended regimens with two types of silicone hydrogel lenses. | 2009 | 19201251 |
myocardial and aortal involvement in a case of disseminated infection with fusarium solani after allogeneic stem cell transplantation: report of a case. | summary we describe a 57-year-old woman suffering from acute erythroblastic leukaemia. after the first course of high-dose ara-c containing consolidation therapy, the patient developed multiple skin lesions on the left foot. a skin biopsy revealed a fusarium infection. the lesions regressed under therapy with caspofungin and voriconazole. leukaemia relapsed after 1 year and an allogeneic stem cell transplantation was performed for consolidation of leukaemia in second remission. again, the patien ... | 2009 | 19207852 |
the impact of arthropods on fungal community structure in lascaux cave. | to determine the major components of the fungal population present in lascaux cave, france. the ceiling, walls, sediments and soil were colonized by fusarium solani in 2001 and later, in 2006, black stains appeared. however, the origin of the successive fungal invasions is unknown as well as the ecology of the cave. | 2009 | 19210566 |
lethal synergy of solar uv-radiation and h(2)o(2) on wild fusarium solani spores in distilled and natural well water. | environmentally-friendly disinfection methods are needed in many industrial applications. as a natural metabolite of many organisms, hydrogen peroxide (h(2)o(2))-based disinfection may be such a method as long as h(2)o(2) is used in non-toxic concentrations. nevertheless, when applied alone as a disinfectant, h(2)o(2) concentrations need to be high enough to achieve significant pathogen reduction, and this may lead to phytotoxicity. this paper shows how h(2)o(2) disinfection concentrations could ... | 2009 | 19217637 |
molecular assays reveal the presence and diversity of genes encoding pea footrot pathogenicity determinants in nectria haematococca and in agricultural soils. | the aim of this study was to develop molecular assays for investigating the presence and diversity of pathogenicity genes from the pea footrot pathogen nectria haematococca (anamorph fusarium solani f.sp. pisi) in soils. | 2009 | 19226389 |
benzo[a]pyrene degradation using simultaneously combined chemical oxidation, biotreatment with fusarium solani and cyclodextrins. | the interest of simultaneously combining chemical (fenton's reaction) and biological treatments for the degradation of a high molecular weight polycyclic aromatic hydrocarbon benzo[a]pyrene (bap) has been studied in laboratory tests. an optimal concentration of 1.5x10(-3) m h(2)o(2) as fenton's reagent was firstly determined as being compatible with the growth of fusarium solani, the deuteromycete fungus used in the biodegradation process. for the enhancement of bap solubilisation, cyclodextrins ... | 2009 | 19246189 |
role for fks1 in the intrinsic echinocandin resistance of fusarium solani as evidenced by hybrid expression in saccharomyces cerevisiae. | the opportunistic mold fusarium solani is intrinsically resistant to cell wall synthesis-inhibiting echinocandins (ecs), including caspofungin and micafungin. mutations that confer acquired ec resistance in saccharomyces cerevisiae and other normally susceptible yeast species have been mapped to the fks1 gene; among these is the mutation of residue 639 from phe to tyr (f639y) within a region designated hot spot 1. fks1 sequence analysis identified the equivalent of y639 in f. solani as well as i ... | 2009 | 19258277 |
microbial conversion and in vitro and in vivo antifungal assessment of bioconverted docosahexaenoic acid (bdha) used against agricultural plant pathogenic fungi. | microbial modification of polyunsaturated fatty acids can often lead to special changes in their structure and in biological potential. therefore, the aim of this study was to develop potential antifungal agents through the microbial conversion of docosahexaenoic acid (dha). bioconverted oil extract of docosahexaenoic acid (bdha), obtained from the microbial conversion of docosahexaenoic acid (dha) by pseudomonas aeruginosa pr3, was assessed for its in vitro and in vivo antifungal potential. myc ... | 2009 | 19259715 |
fungal parasitism of heterodera glycines eggs as influenced by egg age and pre-colonization of cysts by other fungi. | the objective of this study was to determine the effect of egg age and pre-colonization of cysts by a saprophytic or parasitic fungus on parasitism of heterodera glycines eggs by other parasitic fungi. in agar and in soil tests, fungi generally parasitized more eggs in early developmental stages than eggs containing a juvenile. the effect of pre-colonization of cysts by a fungus on parasitism of eggs by other fungi depended on the fungi involved. in most cases, pre-colonization of cysts by an un ... | 2003 | 19262761 |
multipurpose solutions and rates of biocidal efficacy. | to investigate the hypothesis that reasonable levels of disinfection are achievable over relatively short exposure durations of microorganisms to multipurpose solutions, this study examined the stand-alone disinfection efficacy at 10, 20, and 30 min of five different multipurpose solutions products, each against five different pathogens eye care practitioners may encounter in their contact lens practice. | 2009 | 19265330 |
eggs of tylenchulus semipenetrans inhibit growth of phytophthora nicotianae and fusarium solani in vitro. | in previous greenhouse and laboratory studies, citrus seedlings infested with the citrus nematode tylenchulus semipenetrans and later inoculated with the fungus phylophthora nicotianae grew larger and contained less fungal protein in root tissues than plants infected by only the fungus, demonstrating antagonism of the nematode to the fungus. in this study, we determined whether eggs of the citrus nematode t. semipenetrans and root-knot nematode meloidogyne arenaria affected mycelial growth of p. ... | 2002 | 19265943 |
tylenchulus semipenetrans alters the microbial community in the citrus rhizosphere. | infection of citrus seedlings by tylenchulus semipenetrans was shown to reduce subsequent infection of roots by phytophthora nicotianae and to increase plant growth compared to plants infected by only the fungus. hypothetical mechanisms by which the nematode suppresses fungal development include nutrient competition, direct antibiosis, or alteration of the microbial community in the rhizosphere to favor microorganisms antagonistic to p. nicotianae. a test of the last hypothesis was conducted via ... | 2003 | 19265991 |
viability of heterodera glycines exposed to fungal filtrates. | filtrates from nematode-parasitic fungi have been reported to be toxic to plant-parasitic nematodes. our objective was to determine the effects of fungal filtrates on second-stage juveniles and eggs of heterodera glycines. eleven fungal species that were isolated from cysts extracted from a soybean field in florida were tested on j2, and five species were tested on eggs in vitro. each fungal species was grown in czapek-dox broth and malt extract broth. no toxic activity was observed for fungi gr ... | 2000 | 19270965 |
fungal sensitization in nasal polyposis. | there are indications that polyposis is somehow related to allergic phenomena. fungal sensitization in substantial proportions of patients has been cited as a trigger of inflammatory mechanisms involving either an immunoglobulin (ig) e-mediated reaction to fungal colonization or fungal invasion of tissues. | 2009 | 19274923 |
pathogenicity of fungi to eggs of heterodera glycines. | twenty-one isolates of 18 fungal species were tested on water agar for their pathogenicity to eggs of heterodera glycines. an egg-parasitic index (epi) for each of these fungi was recorded on a scale from 0 to 10, and hatch of nematode eggs was determined after exposure to the fungi on water agar for 3 weeks at 24 c. the epi for verticillium chlamydosporium was 7.6, and the fungus reduced hatch 74%. pyrenochaeta terrestris and two sterile fungi also showed a high epi and reduced hatch 42-73%. ar ... | 1996 | 19277130 |
genotypic identification of fusarium species from ocular sources: comparison to morphologic classification and antifungal sensitivity testing (an aos thesis). | ocular infections caused by fungal organisms can cause significant ocular morbidity, particularly when diagnosis and treatment are delayed. rapid and accurate identification of fusarium species at the subgenus level using current diagnostic standards is timely and insensitive. the purpose of this study is to examine the usefulness of polymerase chain reaction (pcr) analysis of the internal transcribed spacer (its) regions (its1, 5.8s, and its2) in detecting and differentiating fusarium species f ... | 2008 | 19277239 |
development of heterodera glycines as affected by fusarium solani, the causal agent of sudden death syndrome of soybean. | the effects of the blue form of fusarium solani, the causal agent of sudden death syndrome (sds), on heterodera glycines were examined in the greenhouse. roots of soybean cv. coker 156 were inoculated with either h. glycines alone or f. solani + h. glycines in combination. population levels of h. glycines were reduced 47% in the presence of f. solani. life-stage development of h. glycines increased 3% in 30 days in the presence of f. solani. fusarium solani colonized epidermal and cortical cells ... | 1995 | 19277263 |
interrelationship of heterodera glycines and fusarium solani in sudden death syndrome of soybean. | experiments were established in field microplots to examine the association between heterodera glycines and the blue form of fusarium solani in sudden death syndrome of soybean (sds). foliar disease symptoms occurred on more plants per plot, appeared 3 to 7 days earlier, and were more severe on plants grown in plots infested with f. solani + h. glycines than on those inoculated with f. solani only. yields were suppressed only in treatments that included the nematode. numbers of h. glycines cysts ... | 1993 | 19279791 |
localized influence of heterodera glycines on sudden death syndrome of soybean. | half-root tests were established to examine the association between heterodera glycines and the blue strain of fusarium solani, the causal agent of sudden death syndrome (sds) of soybean. two independent root systems were established for soybean 'coker 156' and inoculated (half root/half root) with f. solani, h. glycines, both organisms on opposite root halves, both organisms on one root half, or neither one. foliar symptoms were more severe for plants inoculated with both organisms on one root ... | 1993 | 19279825 |
fungi associated with females and cysts of heterodera glycines in a florida soybean field. | fungal colonization was determined for females and cysts of heterodera glycines on soybean roots or in rhizosphere soil from a florida soybean field. a total of 1,620 females and cysts were examined in 1991, and 1,303 were examined in 1992. more than 35 species of fungi were isolated from females and cysts. the frequency of fungi colonizing white and yellow females was low, but a high frequency of fungi was encountered in brown cysts, which increased with time of exposure of the cysts to the soi ... | 1994 | 19279895 |
plant-parasitic nematodes and fungi associated with root rot of peas on prince edward island. | eight commercial pea fields on prince edward island were sampled in june and july over a 2-year period (1986-87) to determine soil population densities and the incidence of nematodes and fungi associated with root rot of peas. root lesion nematodes (pratylenchus spp.) were the dominant endoparasitic nematodes recovered from roots and soil. low populations of the northern root-knot nematode (meloidogyne hapla) were also present. tylenchorhynchus spp. and paratylenchus spp. were recovered frequent ... | 1990 | 19287779 |
utilisation of carbon sources by pythium, phytophthora and fusarium species as determined by biolog microplate assay. | this study examined the metabolic activity of pure cultures of five root pathogens commonly found in closed hydroponic cultivation systems (phytophthora cryptogea (pc), phytophthora capsici (pcp), pythium aphanidermatum (pa), fusariumoxysporum f.sp. radicis-lycopersici (forl) and fusarium solani (fs)) using sole carbon source utilisation in order to develop effective biocontrol strategies against these pathogens. aliquots of 150 microl of the mycelial suspension were inoculated in each well of g ... | 2009 | 19294012 |
association of criconemella xenoplax and fusarium spp. with root necrosis and growth of peach. | criconemella xenoplax, fusarium solani, and f. oxysporum caused necrosis of nemaguard peach feeder roots in greenhouse tests. root necrosis was more extensive in the presence of either fungus than wtih c. xenoplax alone. shoot growth and plant height were less for plants inoculated with f. oxysporum or f. solani than for plants inoculated with the fungi plus c. xenoplax. neither synergistic nor additive effects on root necrosis or plant growth occurred between c. xenoplax and the fungal pathogen ... | 1986 | 19294169 |
effects of management practices on nematode and fungus populations and cucumber yield. | three crops of cucumber were grown in succession in beds by use of trickle irrigation, plastic film mulch, and soil chemical treatments over a 17-month period, including a fallow winter season. total yield for the three crops was highest (1208 quintals/ha) in film-mulched plots treated with mbr-cp, and next-highest in film-mulched plots treated with dd-mencs (1094 quintals/ha); total yield was only 456 quintals/ha in film-mulched control (untreated) plots. yield in untreated film-mulched plots w ... | 1979 | 19305534 |
[fusarium keratitis and endophthalmitis treated by intravenous ambisome]. | this is a case study of fusarium keratitis progressing to endophthatmitis that was successfully treated with a tiposomal formulation of amphotericin b (ambisome] and local natamycin 5%. | 2009 | 19320385 |
tracking the emerging human pathogen pseudallescheria boydii by using highly specific monoclonal antibodies. | pseudallescheria boydii has long been known to cause white grain mycetoma in immunocompetent humans, but it has recently emerged as an opportunistic pathogen of humans, causing potentially fatal invasive infections in immunocompromised individuals and evacuees of natural disasters, such as tsunamis and hurricanes. the diagnosis of p. boydii is problematic since it exhibits morphological characteristics similar to those of other hyaline fungi that cause infectious diseases, such as aspergillus fu ... | 2009 | 19321690 |
biological screening of euphorbia helioscopia l. | the present research is preliminary biological screening of euphorbia helioscopia l. (euphorbiaceae). dichloromethane and methanol extracts of the aerial parts of the plant were investigated for their antioxidant, antifungal, antibacterial and phytotoxic activities. dichloromethane extract exhibited significant activity against fusarium solani with 90% inhibition, where as the same extract also showed non-significant activity against salmonella typhi and bacillus subtilis. methanolic extract has ... | 2009 | 19339230 |
antimicrobial dihydroisocoumarins from crassocephalum biafrae. | bioassay-guided fractionation of the chcl (3)-soluble extract of the stem bark of crassocephalum biafrae (asteraceae) resulted in the isolation of three new dihydroisocoumarins, named biafraecoumarins a ( 1), b ( 2), and c ( 3); two known triterpenes ( 4 and 5); and a known ceramide ( 6). the structures of the new compounds were established as 7-but-15-enyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 1), 7-butyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 2), and 7-butyl- ... | 2009 | 19350487 |
soft contact lens disinfection solution efficacy: clinical fusarium isolates vs. atcc 36031. | to compare the disinfecting efficacy of five soft contact lens multipurpose disinfection solutions (mpds) against fusarium solani clinical isolates and the iso standard atcc 36031 strain. | 2009 | 19384259 |
impact of biocide treatments on the bacterial communities of the lascaux cave. | the lascaux cave contains a remarkable set of paintings from the upper palaeolithic. shortly after discovery in 1940, the cave was modified for public viewing and, in 2001, was invaded by a fusarium solani species complex. benzalkonium chloride was used from 2001 to 2004 to eliminate the fungal outbreak. in this study, we carried out a sampling in most of the cave halls and galleries. sequence analysis and isolation methods detected that the most abundant genera of bacteria were ralstonia and ps ... | 2009 | 19404600 |
chromatographic isolation and characterization of a novel peroxidase from large lima legumes. | a novel peroxidase with antifungal activity was isolated from the large lima bean (phaseolus limensis) seeds. the procedure entailed extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-toyopearl, and gel filtration on sephadex g-75. the enzyme was adsorbed on affi-gel blue gel and sp-toyopearl, and possessed a molecular weight of 34 kda in sds-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions. ... | 2009 | 19408412 |
relative in vitro rates of attachment and penetration of hydrogel soft contact lenses by haplotypes of fusarium. | to investigate the relative abilities of different haplotypes of the fusarium solani (fssc)-fusarium oxysporum (fosc) complexes to attach to and invade hydrogel contact lenses. | 2009 | 19411965 |
synthesis of alkyl esters by cutinase in miniemulsion and organic solvent media. | the main objective of this work was studying and testing the nature and influence of reaction media (organic solvent vs. miniemulsion system) on the synthesis of alkyl esters catalyzed by fusarium solani pisi cutinase. ester synthesis and cutinase selectivity for different chain length of acids and alcohols (ethyl and hexyl) were evaluated. in iso-octane, after 1 h of reaction, cutinase exhibits rates of esterification between 0.24 micromol x mg(-)1 x min(-1) for ethyl oleate and 1.15 micromol x ... | 2009 | 19418474 |
fungal nitrilases as biocatalysts: recent developments. | of the numerous putative fungal nitrilases available from protein databases only a few enzymes were purified and characterized. the purified nitrilases from fusarium solani, fusarium oxysporum f. sp. melonis and aspergillus niger share a preference for (hetero)aromatic nitriles, temperature optima between 40 and 50 degrees c and ph optima in the slightly alkaline region. on the other hand, they differ in their chemoselectivity, i.e. their tendency to produce amides as by-products. the production ... | 2009 | 19427375 |
cutaneous fusarium solani infection in childhood acute lymphoblastic leukaemia. | cutaneous involvement is often an initial presentation of infection with fusarium species, which occurs more commonly in immunocompromised hosts and may be either localized or widespread. skin lesions typically appear as red or grey macules, which may develop central ulceration and black eschar. secondary dissemination to extracutaneous organs may occur in immunocompromised hosts, especially those with prolonged and severe neutropenia. we describe a case of widespread cutaneous involvement after ... | 2009 | 19438533 |
use of mass spectrometry to identify clinical fusarium isolates. | fusarium spp. have recently emerged as significant human pathogens. identification of these species is important, both for epidemiological purposes and for patient management, but conventional identification based on morphological traits is hindered by major phenotypic polymorphism. in this study, 62 strains, or isolates, belonging to nine fusarium species were subjected to both molecular identification tef1 gene sequencing and matrix-assisted laser desorption ionization-time-of-flight (maldi-to ... | 2009 | 19456834 |
fusarium keratitis in mexico. | to describe the clinical characteristics, diagnosis, treatment, and visual outcome of 61 cases of fusarium keratitis in mexico. | 2009 | 19512910 |
crystal structure and enhanced activity of a cutinase-like enzyme from cryptococcus sp. strain s-2. | the structural and enzymatic characteristics of a cutinase-like enzyme (cle) from cryptococcus sp. strain s-2, which exhibits remote homology to a lipolytic enzyme and a cutinase from the fungus fusarium solani (fs cutinase), were compared to investigate the unique substrate specificity of cle. the crystal structure of cle was solved to a 1.05 a resolution. moreover, hydrolysis assays demonstrated the broad specificity of cle for short and long-chain substrates, as well as the preferred specific ... | 2009 | 19544571 |
continuous hydrolysis of 4-cyanopyridine by nitrilases from fusarium solani o1 and aspergillus niger k10. | the operational stabilities of nitrilases from aspergillus niger k10 and fusarium solani o1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (csmrs). the former enzyme was fairly stable at 30 degrees c with a deactivation constant (k (d)) and enzyme half-life of 0.014 h(-1) and 50 h, respectively, but the latter exhibited an even higher stability characterized by k (d) = 0.008 h(-1) and half-life of 87 h at 40 degrees c. another advantage of this enzyme ... | 2009 | 19554325 |
molecular detection of dermatophytes and nondermatophytes in onychomycosis by nested polymerase chain reaction based on 28s ribosomal rna gene sequences. | onychomycosis is often caused by dermatophytes, but the role of nondermatophytes is underestimated due to the difficulty of identifying them by conventional direct microscopy and culture. | 2009 | 19566663 |
[preliminary study on the use of dna suspension array for the identification of corneal pathogenic fungi]. | to develop a multiplex, microsphere-based dna suspension array for the identification of important pathogenic fungi of cornea and to study the feasibility of its application in the clinical diagnosis of fungal keratitis. | 2009 | 19573328 |
antifungal activity of nettle (urtica dioica l.), colocynth (citrullus colocynthis l. schrad), oleander (nerium oleander l.) and konar (ziziphus spina-christi l.) extracts on plants pathogenic fungi. | anti-mycotic activity of the ethanol extracts from nettle (urtica dioica l.), colocynth (citrullus colocynthis l. schrad), konar (ziziphus spina-christi l.) and oleander (nerium oleander l.) floral parts were screened in vitro against four important plant pathogenic fungi viz.; alternaria alternate, fusarium oxysporum, fusarium solani and rizoctonia solani using agar dilution bioassay. extracts showed antifungal activity against all the tested fungi. among the plants, nettle and colocynth were t ... | 2009 | 19579919 |
induce systemic resistance in lupine against root rot diseases. | root rot caused by soil borne pathogenic fungi is the most sever disease attacks lupine plants. isolation trials from diseased plants in some areas of dakahlia province (egypt) was carried out. rhizoctonia solani and fusarium solani proved to be the most dominant isolates. meanwhile, fusarium oxysporum and sclerotium rolfsii were less frequent. efficacies of some plant resistance elicitors viz.: chitosan (chi), salicylic acid (sa) and hydroquinone (hq) in comparing to the fungicide rhizolex t-50 ... | 2009 | 19579949 |
flash photolysis of cutinase: identification and decay kinetics of transient intermediates formed upon uv excitation of aromatic residues. | aromatic amino acids play an important role in ultraviolet (uv)-induced photochemical reactions in proteins. in this work, we aim at gaining insight into the photochemical reactions induced by near-uv light excitation of aromatic residues that lead to breakage of disulfide bridges in our model enzyme, fusarium solani pisi cutinase, a lipolytic enzyme. with this purpose, we acquired transient absorption data of cutinase, with supplemental experimental data on tryptophan (trp) and lysozyme as refe ... | 2009 | 19580759 |
high-level expression and characterization of fusarium solani cutinase in pichia pastoris. | high-level extracellular production of fusarium solani cutinase was achieved using a pichia pastoris expression system. the cutinase-encoding gene was cloned into ppiczalphaa with the saccharomyces cerevisiae alpha-factor signal sequence and methanol-inducible alcohol oxidase promoter by two different ways. the additional sequences of the c-myc epitope and (his)6-tag of the vector were fused to the c-terminus of cutinase, while the other expression vector was constructed without any additional s ... | 2009 | 19580870 |
enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules. | a lipase from thermomyces lanuginosus and cutinases from thermobifida fusca and fusarium solani hydrolysed poly(ethylene terephthalate) (pet) fabrics and films and bis(benzoyloxyethyl) terephthalate (3pet) endo-wise as shown by maldi-tof-ms, lc-uvd/ms, cationic dyeing and xps analysis. due to interfacial activation of the lipase in the presence of triton x-100, a seven-fold increase of hydrolysis products released from 3pet was measured. in the presence of the plasticizer n,n-diethyl-2-phenylace ... | 2009 | 19616594 |
quantitative determination of gibberellins by high performance liquid chromatography from various gibberellins producing fusarium strains. | high performance liquid chromatographic (hplc) method was developed for analysis of seven gibberellins, i.e., ga3, ga4, ga7, ga3 methyl ester, ga7 methyl ester 3,13 diacetate, ga7 methyl ester, and fusaric acid, using an isocratic system. method was used for estimation of gibberellins from different fusarium strains. gibberellins were extracted from 28 strains of fusarium, out of which six strains of fusarium were isolated from soil of different parts of india and 22 strains were procured from t ... | 2010 | 19626451 |
backbone dynamics of the antifungal psd1 pea defensin and its correlation with membrane interaction by nmr spectroscopy. | plant defensins are cysteine-rich cationic peptides, components of the innate immune system. the antifungal sensitivity of certain exemplars was correlated to the level of complex glycosphingolipids in the membrane of fungi strains. psd1 is a 46 amino acid residue defensin isolated from pea seeds which exhibit antifungal activity. its structure is characterized by the so-called cysteine-stabilized alpha/beta motif linked by three loops as determined by two-dimensional nmr. in the present work we ... | 2010 | 19632194 |
[tumour occurrence of minnow phoxinys phoxinus (l.) from the north dvina and pechora river basins]. | tumours occurrence of minnow and tumour parameters are found to be greatest at the end of june--the first part of july. black tumours give metastases, while gray tumours are separated from the neighboring tissue by a capsule. the stroma of the tumour is pierced through by fungal hyphae of fusarium solani (mart.) app. et wr. the minnow living the first year (the end of it) and the second year (the beginning of it) suffers tumours more frequently. the minnow taken from the saitanovka river estuary ... | 2009 | 19637775 |
fungal biomass is a key factor affecting polymorphonuclear leucocyte-induced hyphal damage of filamentous fungi. | previous studies have not systematically assessed the effect of fungal biomass on polymorphonuclear leucocyte (pmn)-induced hyphal damage (hd) of filamentous fungi. we hypothesised that fungal biomass is a significant factor affecting pmn-induced hd. one isolate each consisting of a volume of 2 x 10(4) conidia ml(-1) of aspergillus fumigatus, aspergillus flavus, aspergillus terreus, rhizopus oryzae, rhizopus microsporus, cunninghamella bertholletiae, scedosporium prolificans and fusarium solani ... | 2010 | 19638003 |
improved microwave-mediated synthesis of 3-(3-aryl-1,2,4-oxadiazol-5-yl)propionic acids and their larvicidal and fungal growth inhibitory properties. | the synthesis of 3-(3-aryl-1,2,4-oxadiazol-5-yl)propionic acids from arylamidoximes and succinic anhydride under focused microwave irradiation conditions is described. the new synthetic method furnished the desired products in 2-3 min and good yields. furthermore, the previously complicated purification procedure has been simplified in a manner which is quick, eco-friendly and cost-effective. larvicidal bioassay and fungal growth inhibitory tests were performed using several 3-(3-aryl-1,2,4-oxad ... | 2009 | 19652406 |
hyphal penetration of worn hydrogel contact lenses by fusarium. | to determine the relative susceptibility of worn and unworn hydrogel contact lenses to penetration by hyphae of the fusarium solani-fusarium oxysporum species complex. | 2009 | 19654522 |
methylcitrate cycle activation during adaptation of fusarium solani and fusarium verticillioides to propionyl-coa-generating carbon sources. | propionyl-coa is an inhibitor of both primary and secondary metabolism in aspergillus species and a functional methylcitrate cycle is essential for the efficient removal of this potentially toxic metabolite. although the genomes of most sequenced fungal species appear to contain genes coding for enzymes of the methylcitrate cycle, experimental confirmation of pathway activity in filamentous fungi has only been provided for aspergillus nidulans and aspergillus fumigatus. in this study we demonstr ... | 2009 | 19661181 |
invasive fusariosis with prolonged fungemia in a patient with acute lymphoblastic leukemia: case report and review of the literature. | fusariumspp are rare but important opportunistic pathogens in immunocompromised patients. disseminated fusarial infections occur mostly in patients with hematologic malignancies with myelosuppressive chemotherapy or in patients with severe immunodeficiency. although more frequent than aspergillus fungemia, fusarium fungemia remains a rare event. we describe the case of a female patient with febrile neutropenia and persistent fungemia due to fusarium solani, treated with posaconazole and liposoma ... | 2010 | 19665409 |
in the aftermath of the fusarium keratitis outbreak: what have we learned? | the 2005-2006 outbreak of fusarium keratitis associated with soft hydrophilic contact lens wear was both unprecedented and unexpected. more than 250 cases have been reported worldwide that have primarily been associated with bausch & lomb renu with moistureloc and, more recently, with bausch and lomb renu multiplus multipurpose contact lens disinfecting solutions. this article documents the outbreak, presenting the time line and the historical and scientific basis for its occurrence. underlying ... | 2007 | 19668512 |
the genome of nectria haematococca: contribution of supernumerary chromosomes to gene expansion. | the ascomycetous fungus nectria haematococca, (asexual name fusarium solani), is a member of a group of >50 species known as the "fusarium solani species complex". members of this complex have diverse biological properties including the ability to cause disease on >100 genera of plants and opportunistic infections in humans. the current research analyzed the most extensively studied member of this complex, n. haematococca mating population vi (mpvi). several genes controlling the ability of indi ... | 2009 | 19714214 |
isotope fractionations in the biosynthesis of cell components by different fungi: a basis for environmental carbon flux studies. | abstract the isotope fractionation of carbon from substrates possessing different isotope ratios into fatty acids of polar lipids and amino acids was determined for four different fungi (rhizopus arrhizus, mortierella isabellina, fusarium solani, aspergillus niger). carbon isotope ratios of fungi closely followed that of the substrates. palmitic acid (c16:0), derived from phospholipids, did not display a large carbon isotope fractionation against the substrate. stearic acid (c18:0), however, was ... | 2003 | 19719589 |
identification of two gh18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete aphanomyces astaci. | the oomycete aphanomyces astaci is regarded as the causative agent of crayfish plague and represents an evident hazard for european crayfish species. native crayfish populations infected with this pathogen suffer up to 100% mortality. the existence of multiple transmission paths necessitates the development of a reliable, robust and efficient test to detect the pathogen. currently, a. astaci is diagnosed by a pcr-based assay that suffers from cross-reactivity to other species. we developed an al ... | 2009 | 19719847 |