Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| atypical bacterial pathogen infection in children with acute bronchiolitis in northeast thailand. | atypical bacterial pathogens--including mycoplasma pneumoniae, chlamydophila pneumoniae, and chlamydia trachomatis--are important infectious agents of the respiratory system. most current information pertains to adults and little is known about the role of these organisms in lower respiratory tract infections among young children with acute bronchiolitis. | 2011 | 21439510 |
| lung function prior to viral lower respiratory tract infections in prematurely born infants. | prematurely born infants who develop respiratory syncytial virus (rsv) lower respiratory tract infections (lrtis) have lung function abnormalities at follow-up. the aim of this study was to determine whether prematurely born infants who developed symptomatic rsv, or other viral lrti(s), had poorer premorbid lung function than infants who did not develop lrtis during the rsv season. | 2011 | 21447496 |
| in vitro sensitivity of human metapneumovirus to type i interferons. | human metapneumovirus (hmpv) has been recognized as an important respiratory pathogen. due to its relatively recent discovery, only limited information is available on the relationship between hmpv and type i interferons (ifn). this study was designed to determine whether in vitro hmpv is sensitive to the antiviral activity of ifn-ß, leukocyte ifn-a, and several ifn-a subtypes in a human hep-2 cell line. the results showed that 50% inhibitory concentration values against hmpv for the various typ ... | 2011 | 21449726 |
| comparison of human metapneumovirus and respiratory syncytial virus in children admitted to a paediatric intensive care unit. | aim: to describe the clinical presentation and course of children admitted to the paediatric intensive care unit (picu) with human metapneumovirus (hmpv) infection, and compare them with children admitted to the picu with respiratory syncytial virus (rsv) infection. methods: hmpv was identified by immunofluorescence in 22 children admitted to the picu over a 16-month period. the medical records of these children were reviewed retrospectively, and their clinical and laboratory data were compare ... | 2011 | 21449904 |
| effects of n-linked glycosylation of the fusion protein on replication of human metapneumovirus in vitro and in mouse lungs. | the fusion (f) protein is an important membrane glycoprotein necessary for cellular entry and replication of human metapneumovirus (hmpv). selective prevention of n-linked glycosylation may compromise the catalytic and fusion functions of the f protein. by using site-directed mutagenesis and reverse genetics, recombinant mutant viruses lacking one or two n-linked glycosylation sites in the f protein were constructed. m1, which lacked glycosylation at position 57 of the f protein, had slightly co ... | 2011 | 21450943 |
| viral etiologies of acute respiratory infections among hospitalized vietnamese children in ho chi minh city, 2004-2008. | the dominant viral etiologies responsible for acute respiratory infections (aris) are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by aris are highest. improved etiological insight is needed to improve clinical management and prevention. | 2011 | 21455313 |
| viral infections of the lower respiratory tract: old viruses, new viruses, and the role of diagnosis. | viral infections of the lower respiratory tract cause an enormous disease burden in children, and the role of respiratory viruses in serious lower respiratory tract infections (lrtis) in older adults is increasingly appreciated. although viruses are responsible for a large proportion lrtis, antibiotics are often prescribed. new diagnostic platforms have the potential to detect a wider range of established and newly discovered viruses with greater sensitivity. this will create additional challeng ... | 2011 | 21460286 |
| detection of human metapneumovirus in infants with acute respiratory tract infection. | both respiratory syncytial virus (rsv) and human metapneumovirus (hmpv), a recently discovered respiratory pathogen classified in the paramyxoviridae family, have been associated with acute respiratory tract infections (arti) in infants. the aim of our study was to determine the incidence and clinical features of hmpv infection in infants presenting with arti at a tertiary children's hospital in greece during the 2007-2008 and 2008-2009 winter seasons. routine nasal swabs were obtained from 127 ... | 2011 | 21468562 |
| human metapneumovirus infection in wild mountain gorillas, rwanda. | the genetic relatedness of mountain gorillas and humans has led to concerns about interspecies transmission of infectious agents. human-to-gorilla transmission may explain human metapneumovirus in 2 wild mountain gorillas that died during a respiratory disease outbreak in rwanda in 2009. surveillance is needed to ensure survival of these critically endangered animals. | 2011 | 21470468 |
| [clinical characteristics of human bocavirus infection in 90 children]. | to study the clinical characteristics of human bocavirus (hbov) infection in children. | 2011 | 21507299 |
| a comparison of two multiplex methods for the detection of respiratory viruses: filmarray rp and xtag rvp. | we compared the filmarray rp (idaho technology, inc. salt lake city, utah) with the xtag rvp (luminex corporation, toronto, canada)multiplex respiratory virus pcr methods for the detection of respiratory viruses in a set of 200 patient specimens frozen at - 70° c after standard viral culture and antigen detection methods were done. both systems detected between 40 - 50% more viruses than traditional methods, primarily rhinoviruses and human metapneumovirus. the filmarray rp detected significantl ... | 2011 | 21508156 |
| respiratory viruses in lung transplant recipients: a critical review and pooled analysis of clinical studies. | lung transplant recipients present an increased risk for severe complications associated with respiratory infections. we conducted a review of the literature examining the clinical relationship between viral respiratory infection and graft complications. thirty-four studies describing the clinical impact of influenza, respiratory syncytial virus, parainfluenza, human metapneumovirus, rhinovirus, enterovirus, coronavirus, bocavirus or adenovirus were identified. the detection rate of respiratory ... | 2011 | 21521473 |
| viral etiology of acute lower respiratory tract infections in hospitalized young children in northern taiwan. | lower respiratory tract infections (lrtis) comprise a great proportion of diagnoses among hospitalized children. this study identifies the viral pathogens causing lrtis in young children and compares their clinical features and disease severity. | 2011 | 21524612 |
| the conserved yagl motif in the human metapneumovirus is required for higher ordered cellular assemblies of the matrix protein and for virion production. | yxxl motifs in cellular and viral proteins have variety of functions. the matrix (m) protein of the respiratory pathogen - the human metapneumovirus (hmpv) contains two such conserved motifs - yskl and yagl. we mutated these sequences to analyze their contribution to hmpv infectivity. the mutant clones were capable of intracellular replication; however, the yagl, but not the yskl, mutants were spread-defective in infected cultures. we improved the reverse genetics system for hmpv and generated c ... | 2011 | 21525358 |
| [prokaryotic expression for fusion protein of human metapneumovirus and its preliminary application as an antigen for antibody detection]. | to understand the effectiveness of prokaryotic expression of fusion protein (f) of human metapneumovirus (hmpv) and its application as antigen, f proteins from different genotypes of hmpv were expressed in prokaryotic expression system and purified by ni-nta affinity chromatography column. according to the hydrophobicity, antigen index and surface probability of f protein, the subunit 1 (f1) region of f protein was generated and expressed in e. coil. bl21(de3). the 6-his-f1 proteins with molecul ... | 2011 | 21528539 |
| viral and atypical bacterial detection in acute respiratory infection in children under five years. | acute respiratory infection (ari) is a leading cause of morbidity and mortality in children worldwide. this study aimed to determine the viral and atypical bacterial causes of different severities and clinical manifestations of ari in preschool children from low-income families in north-east brazil. | 2011 | 21533115 |
| detection of ki polyomavirus and wu polyomavirus dna by real-time pcr in nasopharyngeal swabs and in normal lung and lung adenocarcinoma tissues. | polyomaviruses ki (kipyv) and wu (wupyv) were detected from 7 (3.0%) and 38 (16.4%) of 232 children with respiratory tract infections by real-time pcr. the rates of single infection of kipyv and wupyv were 3 (42.9%) of 7 and 20 (52.6%) of 38, respectively. in other samples, various viruses (human respiratory syncytial virus, human metapneumovirus (hmpv), human rhinovirus, parainfluenza virus 1 and human bocavirus) were detected simultaneously. one case was positive for kipyv and wupyv and also f ... | 2011 | 21545509 |
| [characteristics of human metapneumovirus respiratory tract infection in children and the relationship between the infection and meteorological conditions]. | to study the epidemiology of human metapneumovirus (hmpv) infection in children and its relations with meteorological conditions in suzhou. | 2011 | 21575373 |
| management of upper respiratory tract infections in children. | upper respiratory tract infection (urti) occurs commonly in both children and adults and is a major cause of mild morbidity. it has a high cost to society, being responsible for absenteeism from school and work and unnecessary medical care, and is occasionally associated with serious sequelae. urtis are usually caused by several families of virus; these are the rhinovirus, coronavirus, parainfluenza, respiratory syncytial virus (rsv), adenovirus, human metapneumovirus, influenza, enterovirus and ... | 2008 | 21603094 |
| [characteristics and the prevalence of respiratory viruses and the correlation with climatic factors of hospitalized children in suzhou children's hospital.] | objective: to study the epidemiological characteristics of respiratory virus infection and its relations to climatic factors in suzhou. methods: from 2006 to 2009, viral etiology surveillance was conducted among 6655 children hospitalized with acute respiratory tract infections (aris). direct immunofluorescence method was used to test respiratory secretion samples for respiratory syncytial virus (rsv), influenza viruses a and b (inf-a, inf-b), parainfluenza virus types i, ii, and iii (pinf-i, pi ... | 2011 | 21624230 |
| the human metapneumovirus: a case series and review of the literature. | s. shahda, w.g. carlos, p.j. kiel, b.a. khan, c.a. hage. the human metapneumovirus: a case series and review of the literature transpl infect dis 2011: 13: 324-328. all rights reserved abstract: human metapneumovirus (hmpv) is an emerging human pulmonary pathogen that is genetically related to respiratory syncytial virus. it has been increasingly associated with respiratory illnesses over the last few decades. immunocompromised patients are particularly susceptible with resultant morbidity and m ... | 2011 | 21631655 |
| human metapneumovirus glycoprotein g inhibits tlr4-dependent signaling in monocyte-derived dendritic cells. | human metapneumovirus (hmpv) is a major cause of upper and lower respiratory infections in children and adults. recent work from our group demonstrated that hmpv g glycoprotein is an important virulence factor, responsible for inhibiting innate immune responses in airway epithelial cells. myeloid dendritic cells (dcs) are potent apcs and play a major role in initiating and modulating the innate and adaptive immune responses. in this study, we found that tlr4 plays a major role in hmpv-induced ac ... | 2011 | 21632720 |
| human metapneumovirus and human bocavirus associated with respiratory infection in apulian population. | we have studied the occurrence of hbov, hmpv and infa-b in an apulian population with respiratory tract infections. during influenza season 2008-2009, 116 oropharingeal swabs were collected from patients affected by influenza-like illness (ili). the pcr products of hmpv m and hbov np-1 genes were sequenced. 78 out of 116 samples were positive for at least one respiratory virus; hbov was detected in 53, hmpv in 22 and infa-b in 41 out of 116 swabs. a high rate of hbov infection in adult (18.9%) a ... | 2011 | 21636105 |
| viral agents causing lower respiratory tract infections in hospitalized children: evaluation of the speed-oligo® rsv assay for the detection of respiratory syncytial virus. | respiratory syncytial virus (rsv) is the viral agent which is more frequently involved in lower respiratory tract infections (lrtis) in infants under 1 year of age in developed countries. a new oligochromatographic assay, speed-oligo® rsv, was designed and optimized for the specific detection and identification of rsv subtypes a and b. the test was evaluated in 289 clinical samples from 169 hospitalized children using an immunochromatography (ic) test, virus isolation by culture, and an in-house ... | 2011 | 21647616 |
| sole infection by human metapneumovirus among children with radiographically diagnosed community-acquired pneumonia in a tropical region. | limited information is available on the role of human metapneumovirus (hmpv) as the unique pathogen among children hospitalized for community-acquired pneumonia (cap) in a tropical region. | 2011 | 21651739 |
| severity of viral coinfection in hospitalized infants with respiratory syncytial virus infection. | to compare the severity of single respiratory syncytial virus (rsv) infections with that of coinfections. | 2011 | 21655684 |
| a molecular epidemiological study of respiratory viruses detected in japanese children with acute wheezing illness. | recent studies strongly suggest that some respiratory viruses are associated with the induction of acute wheezing and/or exacerbation of bronchial asthma. however, molecular epidemiology of these viruses is not exactly known. | 2011 | 21663657 |
| changing epidemiology of respiratory viral infections in hematopoietic cell transplant recipients and solid organ transplant recipients. | new respiratory viruses have been discovered in recent years and new molecular diagnostic assays have been developed that improve our understanding of respiratory virus infections. this article will review the changing epidemiology of these viruses after hematopoietic stem cell and solid organ transplantation. | 2011 | 21666460 |
| multiple versus single virus respiratory infections: viral load and clinical disease severity in hospitalized children. | please cite this paper as: martin et┬áal. (2011) multiple versus single virus respiratory infections: viral load and clinical disease severity in hospitalized children. influenza and other respiratory viruses. doi: 10.1111/j.1750-2659.2011.00265.x00 (00), 000-000. backgroundôçé molecular testing for viral pathogens has resulted in increasing detection of multiple viruses in respiratory secretions of ill children. the clinical impact of multiple virus infections on clinical presentation and outco ... | 2011 | 21668660 |
| epidemiological and clinical features of respiratory viral infections in hospitalized children during the circulation of influenza virus a(h1n1) 2009. | please cite this paper as: zuccotti et┬áal. (2011) epidemiological and clinical features of respiratory viral infections in hospitalized children during the circulation of influenza virus a(h1n1) 2009. influenza and other respiratory viruses. doi: 10.1111/j.1750-2659.2011.00263.x. backgroundôçé seasonal influenza viruses and respiratory syncytial virus (rsv) are primary causes of acute respiratory tract infections (artis) in children. new respiratory viruses including human metapneumovirus (hmpv ... | 2011 | 21668662 |
| respiratory virus infections among hospitalized patients with suspected influenza a h1n1 2009 virus during the first pandemic wave in brazil. | during the first pandemic wave of the influenza a h1n1 2009 virus, morbidity was particularly high in brazil. hospitalizations resulting from severe respiratory disease due to suspected influenza-like illness created an opportunity to identify other respiratory viruses causing lower respiratory infections. | 2011 | 21670921 |
| treatment with oral ribavirin and ivig of severe human metapneumovirus pneumonia (hmpv) in immune compromised child. | 2011 | 21671370 | |
| wu polyomavirus infection among children in south china. | this study aimed at investigating the prevalence and clinical characteristics of children with respiratory infection by wu polyomavirus (wupyv) in southern china. nasopharyngeal aspirate samples were collected from 771 children with acute respiratory tract infection admitted to hospital and 82 samples from healthy subjects for routine examination at the outpatient service at the second affiliated hospital of shantou university, medical college from july 2008 to june 2009. wupyv was detected by t ... | 2011 | 21678448 |
| contribution of common and recently described respiratory viruses to annual hospitalizations in children in south africa. | the contribution of viruses to lower respiratory tract disease in sub-saharan africa where human immunodeficiency virus may exacerbate respiratory infections is not well defined. no data exist on some of these viruses for southern africa. comprehensive molecular screening may define the role of these viruses as single and co-infections in a population with a high hiv-aids burden. to address this, children less than 5 years of age with respiratory infections from 3 public sector hospitals, pretor ... | 2011 | 21678450 |
| viral acute respiratory infections among infants visited in a rural hospital of southern mozambique. | objectivesôçé to determine the epidemiology and clinical presentation of virus-associated acute respiratory infections (ari) in mozambican infants. methodsôçé a systematic selection of nasopharyngeal aspirates (nôçâ=ôçâ333), collected from infants younger than 12ôçâmonths who visited manhi+ºa district hospital (southern mozambique) with ari during a 12ôçâmonths respiratory syncitial virus surveillance, were tested for other common respiratory viruses. four different polymerase chain reactions we ... | 2011 | 21707876 |
| real-world comparison of two molecular methods for detection of respiratory viruses. | abstract: | 2011 | 21714915 |
| porphyromonas gingivalis enzymes enhance infection with human metapneumovirus in vitro. | relatively recently discovered, the human metapneumovirus (hmpv) is a human pathogen with worldwide prevalence, accounting for a substantial percentage of respiratory tract diseases. concurrent viral and bacterial infections enable intricate mechanisms of cooperation between pathogens, which complicate the symptoms and outcome of the disease. such bilateral interactions are based on the modulation of bacterial growth on epithelium pathologically altered during viral illness and the modulation of ... | 2011 | 21715599 |
| [respiratory infections caused by metapneumovirus in elderly patients]. | human metapneumovirus infections are increasingly recognized among adult patients and the aim of this report is to present a series of 4 cases admitted during the winter of 2010. all were detected by direct fluorescence anti-bodies assay of respiratory samples and all were female patients with an age range of 79 to 95 years, including two bedridden cases, one with dementia and three with chronic obstructive pulmonary disease. one patient presented with parainfluenza 3 virus coinfection. patients ... | 2011 | 21720698 |
| low ccr7-mediated migration of human monocyte derived dendritic cells in response to human respiratory syncytial virus and human metapneumovirus. | human respiratory syncytial virus (hrsv) and, to a lesser extent, human metapneumovirus (hmpv) and human parainfluenza virus type 3 (hpiv3), can re-infect symptomatically throughout life without significant antigenic change, suggestive of incomplete or short-lived immunity. in contrast, re-infection by influenza a virus (iav) largely depends on antigenic change, suggestive of more complete immunity. antigen presentation by dendritic cells (dc) is critical in initiating the adaptive immune respon ... | 2011 | 21731495 |
| comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription-pcr assays. | many acute respiratory illness surveillance systems collect and test nasopharyngeal (np) and/or oropharyngeal (op) swab specimens, yet there are few studies assessing the relative measures of performance for np versus op specimens. | 2011 | 21738731 |
| characterization of human metapneumovirus from pediatric patients with acute respiratory infections in a 4-year period in beijing, china. | human metapneumovirus (hmpv) was discovered by scientists in the netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections (arti) in china. the objective of this study was to determine the importance of hmpv infection in children in beijing and the genotypes of the circulating virus by the surveillance during a four-consecutive-year period. | 2011 | 21740766 |
| genomic analysis of four human metapneumovirus prototypes. | human metapneumovirus (hmpv) is an important cause of acute respiratory illness in children. we determined the complete genome sequence of four strains of hmpv representing each of the four lineages. these sequences were compared with published hmpv genome sequences. most genes were conserved between the genetic lineages (79.5-99.6%), though nucleotide diversity was greater than amino acid diversity, suggesting functional constraints on mutation. however, the sh and g open reading frames were mo ... | 2011 | 21740936 |
| new respiratory viruses and the elderly. | the diagnostics of respiratory viral infections has improved markedly during the last 15 years with the development of pcr techniques. since 1997, several new respiratory viruses and their subgroups have been discovered: influenza a viruses h5n1 and h1n1, human metapneumovirus, coronaviruses sars, nl63 and hku1, human bocavirus, human rhinoviruses c and d and potential respiratory pathogens, the ki and wu polyomaviruses and the torque teno virus. the detection of previously known viruses has als ... | 2011 | 21760867 |
| [molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in nanjing city]. | to investigate the molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in nanjing city, china. | 2011 | 21789843 |
| generation of recombinant metapneumovirus nucleocapsid protein as nucleocapsid-like particles and development of virus-specific monoclonal antibodies. | human metapneumovirus (hmpv) is a member of the pneumovirinea subfamily within the paramyxoviridea family. since its discovery in 2001, hmpv has been isolated in several continents, which suggests its prevalence worldwide. hmpv resembles human respiratory syncytial virus with regard to disease symptoms and its ability to infect and cause disease in young infants as well as individuals of all ages. the aim of the current study was to construct an efficient high-level yeast expression system for t ... | 2011 | 21827798 |
| RSV 2010: Recent advances in research on respiratory syncytial virus and other pneumoviruses. | Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) are important causes of acute respiratory tract disease in infants, immunocompromised patients and the elderly. The Seventh International RSV symposium was held in Rotterdam, the Netherlands, from December 2-5, 2010. This symposium is the flagship event for leading investigators engaged in RSV and HMPV research around the world. The objective of the symposium was to provide a forum to review recent advances in research on RSV, HM ... | 2011 | 21827813 |
| detection and genetic diversity of human metapneumovirus in hospitalized children with acute respiratory infections in india. | human metapneumovirus (hmpv) causes acute respiratory infections in children and adults. it is classified into two major genetic lineages and each lineage into two sublineages. the purpose of the study was to identify and characterize hmpv in children who presented to the all india institute of medical sciences, new delhi, india with acute respiratory infection from april 2005 to march 2007. by reverse-transcription polymerase chain reaction, hmpv was detected in 21 (3%) of the 662 nasopharyngea ... | 2011 | 21837798 |
| human metapneumovirus in patients with respiratory tract infection in kuwait. | human metapneumovirus (hmpv) has been recognized as an important cause of respiratory tract infections in all age groups and in all geographical area. the role of hmpv in causing respiratory tract infections in kuwait was not yet investigated. the aim of this study was to determine the prevalence of hmpv infection in kuwait among patients with respiratory tract infection with respect to other respiratory viruses. during january-december 2009, 460 respiratory samples from 388 patients with respir ... | 2011 | 21837799 |
| Viruses and bacteria in sputum samples of children with community-acquired pneumonia. | Clin Microbiol Infect ABSTRACT: Few comprehensive studies have searched for viruses and bacteria in children with community-acquired pneumonia (CAP). We identified 76 children hospitalized for pneumonia. Induced sputum samples were analysed for 18 viruses by antigen detection and PCR, and for six bacteria by culture and PCR. Viruses were found in 72% of samples, bacteria in 91%, and both in 66%. Rhinovirus (30%), human bocavirus (18%) and human metapneumovirus (14%) were the most commonly detect ... | 2011 | 21851481 |
| identification of potential human respiratory syncytial virus and metapneumovirus t cell epitopes using computational prediction and mhc binding assays. | human respiratory syncytial virus (rsv) and human metapneumovirus (mpv) are two of the most common causes of serious viral lower respiratory tract illness in humans. cd8+ t cells have been shown to be important in animal models and human clinical studies for the clearance of viral infection, and they may contribute in part to protection against severe disease during reinfections. precise enumeration and accurate phenotyping of rsv- or mpv-specific cd8+ t cells in humans is currently limited by t ... | 2011 | 21854782 |
| Mycoplasma pneumoniae and respiratory virus infections in children with persistent cough in England: a retrospective analysis. | Persistent cough following an acute respiratory tract infection is common in children, but clinicians may find it difficult to give accurate prognostic information on likely duration of cough without a microbiologic diagnosis. This study estimates the prevalence of Mycoplasma pneumoniae (Mp) and assesses the prognostic value of detecting Mp and respiratory viruses in children with persistent cough. | 2011 | 21857262 |
| human metapneumovirus. | human metapneumovirus (hmpv) is a common pathogen that can cause both upper and lower respiratory tract infections, particularly in children, elderly adults, and immunocompromised hosts. since its initial identification in 2001, hmpv has been isolated from individuals with acute respiratory tract infections (artis) in virtually every continent. serological studies indicate that it has caused human infection since 1958 or earlier. the epidemiology and clinical manifestations of hmpv are similar t ... | 2011 | 21858749 |
| Respiratory viral infections in hematopoietic stem cell and solid organ transplant recipients. | Respiratory viral infections (RVIs) are common causes of mild illness in immunocompetent children and adults with rare occurrences of significant morbidity or mortality. Complications are more common in the very young, very old, and those with underlying lung diseases. However, RVIs are increasingly recognized as a cause of morbidity and mortality in recipients of hematopoietic stem cell transplants (HSCT) and solid organ transplants (SOTs). Diagnostic techniques for respiratory syncytial virus ... | 2011 | 21858751 |
| rna viruses in young nepalese children hospitalized with severe pneumonia. | pneumonia is among the leading causes of illness and death in children <5 years of age worldwide. there is little information on the viral etiology of severe pneumonia in low-income countries, where the disease burden is particularly high. | 2011 | 21860338 |
| seasonal variations of 15 respiratory agents illustrated by the application of a multiplex polymerase chain reaction assay. | abstract background: nucleic acid amplification tests are increasingly being used to diagnose viral and bacterial respiratory tract infections. the high sensitivity of these tests affects our understanding of the epidemiology of respiratory tract infections. we have assessed the detection rate of a multiplex real-time polymerase chain reaction (pcr) test, with emphasis on epidemiology and seasonal distribution of the most common respiratory tract infections. methods: seven thousand eight hundr ... | 2012 | 21867470 |
| disease burden of the most commonly detected respiratory viruses in hospitalized patients calculated using the disability adjusted life year (daly) model. | the most common acute infections occur in the respiratory tract. recent discoveries of several novel viruses have markedly increased the repertoire of agents understood to cause presentations of acute respiratory disease. | 2011 | 21880543 |
| Streptococcus pneumoniae exposure is associated with human metapneumovirus seroconversion and increased susceptibility to in vitro HMPV infection. | It remains largely unknown which factors determine the clinical outcome of human metapneumovirus (HMPV) infections. The aim of the present study was to analyse whether exposure to bacterial pathogens can influence HMPV infections. From 57 children, serum samples and colonization data for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and Streptococcus pneumoniae were collected at 1.5, 6, 14 and 24 months of age. Seroconversion rates to HMPV were determined and related to ba ... | 2011 | 21883660 |
| viral-bacterial interactions and risk of acute otitis media complicating upper respiratory tract infection. | acute otitis media (aom) is a common complication of upper respiratory tract infection whose pathogenesis involves both viruses and bacteria. we examined risks of acute otitis media associated with specific combinations of respiratory viruses and acute otitis media bacterial pathogens. data were from a prospective study of children ages 6 to 36 months and included viral and bacterial culture and quantitative pcr for respiratory syncytial virus (rsv), human bocavirus, and human metapneumovirus. r ... | 2011 | 21900518 |
| Viral aetiology of influenza-like illness in Belgium during the influenza A(H1N1)2009 pandemic. | The purpose of this investigation was to determine the proportion of influenza-like illness (ILI) attributable to specific viruses during the influenza A(H1N1)2009 pandemic and to describe the demographic and clinical characteristics of ILI due to respiratory viruses in Belgium. Nasopharyngeal swabs were collected from ILI patients by general practitioners (GPs) and paediatricians (PediSurv) and analysed for viruses. Of 139 samples collected from children <5 years of age by PediSurv, 86 were pos ... | 2011 | 21901635 |
| human metapneumovirus and human coronavirus infection and pathogenicity in saudi children hospitalized with acute respiratory illness. | human metapneumovirus (hmpv) and the netherlands human coronavirus (hcov-nl63) have been isolated from children with respiratory tract infection. the prevalence of these viruses has not been reported from saudi arabia. we sought to determine whether hmpv and hcov-nl63 are responsible for acute respiratory illness and also to determine clinical features and severity of illness in the hospitalized pediatric patient population. | 2011 | 21911992 |
| high detection rates of nucleic acids of a wide range of respiratory viruses in the nasopharynx and the middle ear of children with a history of recurrent acute otitis media. | both bacteria and viruses play a role in the development of acute otitis media, however, the importance of specific viruses is unclear. in this study molecular methods were used to determine the presence of nucleic acids of human rhinoviruses (hrv; types a, b, and c), respiratory syncytial viruses (rsv; types a and b), bocavirus (hbov), adenovirus, enterovirus, coronaviruses (229e, hku1, nl63, and oc43), influenza viruses (types a, b, and c), parainfluenza viruses (types 1, 2, 3, 4a, and 4b), hu ... | 2011 | 21915878 |
| recombinant expression and immunological characterisation of proteins derived from human metapneumovirus. | human metapneumovirus (hmpv) has been shown to cause respiratory infection, accounting for approximately 7% of all such disease, and contributes to the development of asthma in humans. hmpv has a worldwide distribution with infectivity rates approaching 100%, and immunocompromised patients are particularly at risk from viral exposure. no anti-hmpv vaccine is available and diagnosis is primarily based on in-house molecular or serological tests, in part due to limited availability of recombinant h ... | 2011 | 21920812 |
| Residues of the human metapneumovirus fusion (F) protein critical for its strain-related fusion phenotype: implications for the virus replication cycle. | The paramyxovirus F protein promotes fusion of the viral and cell membranes for virus entry, as well as cell-cell fusion for syncytium formation. Most paramyxovirus F proteins are triggered at neutral pH to initiate membrane fusion. Previous studies, however, demonstrated that human metapneumovirus (hMPV) F proteins are triggered at neutral or acidic pH in transfected cells, depending on the strain origin of the F sequences (S. Herfst et al., J. Virol. 82:8891-8895, 2008). We now report an exten ... | 2011 | 21937649 |
| human metapneumovirus inhibits ifn-β signaling by downregulating jak1 and tyk2 cellular levels. | human metapneumovirus (hmpv), a leading cause of respiratory tract infections in infants, inhibits type i interferon (ifn) signaling by an unidentified mechanism. in this study, we showed that infection of airway epithelial cells with hmpv decreased cellular level of janus tyrosine kinase (jak1) and tyrosine kinase 2 (tyk2), due to enhanced proteosomal degradation and reduced gene transcription. in addition, hmpv infection also reduced the surface expression of type i ifn receptor (ifnar). these ... | 2011 | 21949722 |
| Respiratory viruses in children with cystic fibrosis: viral detection and clinical findings. | Please cite this paper as: Burns et al. (2011) Respiratory viruses in children with cystic fibrosis: viral detection and clinical findings. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00292.x. Background Viral detection from different respiratory sample types in children with cystic fibrosis (CF) is facilitated by available molecular methods, but optimum sampling strategies have not been identified. In addition, associations between viral detection and respiratory symp ... | 2011 | 21955319 |
| Human Metapneumovirus: lessons learned over the first decade. | It has been 10 years since human metapneumovirus (HMPV) was identified as a causative agent of respiratory illness in humans. Since then, numerous studies have contributed to a substantial body of knowledge on many aspects of HMPV. This review summarizes our current knowledge on HMPV, HMPV disease pathogenesis, and disease intervention strategies and identifies a number of areas with key questions to be addressed in the future. | 2011 | 21976607 |
| development of real-time rt-pcr for human metapneumovirus. | 2011 | 21978165 | |
| The post-infection outcomes of influenza and acute respiratory infection in patients above 50 years of age in Japan: an observational study. | Please cite this paper as: Ikematsu et al. (2011) The post-infection outcomes of influenza and acute respiratory infection in patients above 50 years of age in Japan: an observational study. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00296.x. Objectives Influenza can be a serious illness, especially for older people, and reducing the impact of influenza in elderly is important. The objective of this study was to estimate the prevalence and postinfection outcomes of in ... | 2011 | 21985038 |
| an economical tandem multiplex real-time pcr technique for the detection of a comprehensive range of respiratory pathogens. | this study used real-time pcr assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex pcr. supplementary semi-nested human metapneumovirus and picornavirus pcr assays were required to complete the acute respiratory pathogen profile. potential pathogens were detected in 85 (70%) of pernasal aspirates collected from 121 children with acu ... | 2009 | 21994537 |
| comparison of the filmarray respiratory panel and prodesse real-time pcr assays for detection of respiratory pathogens. | we compared the diagnostic performance and overall respiratory pathogen detection rate of the premarket version of the filmarray respiratory panel (rp) multiplex pcr assay (idaho technology, inc., salt lake city, ut) with those of the food and drug administration (fda)-cleared prodesse proflu+, profast+, proparaflu+, pro hmpv+, and proadeno+ real-time pcr assays (gen-probe, san diego, ca). the assays were performed on a panel of 192 nasopharyngeal-secretion specimens collected from 81 children u ... | 2011 | 21998418 |
| respiratory syncytial virus (rsv) community-acquired pneumonia (cap) in a hospitalized adult with human immunodeficiency virus (hiv) mimicking influenza a and pneumocystis (carinii) jiroveci pneumonia (pcp). | respiratory syncytial virus (rsv) is an important cause of lower respiratory tract infections in young children, the elderly, and immunocompromised hosts, but rsv is a rare cause of community-acquired pneumonia (cap) in hospitalized adults with human immunodeficiency virus (hiv). in patients with hiv, cap is most frequently attributable to the usual bacterial respiratory pathogens that cause cap in immunocompetent hosts, eg, streptococcuspneumoniae or hemophilus influenzae. adults with hiv are a ... | 2012 | 22005289 |
| specific viruses detected in nigerian children in association with acute respiratory disease. | occurrence of different viruses in acute respiratory tract infections of nigerian children was examined. respiratory swabs were collected from 246 children referred to hospital clinics because of acute respiratory symptoms from february through may 2009. validated real-time rt-pcr techniques revealed nucleic acids of at least one virus group in 189 specimens (77%). human rhinoviruses and parainfluenza viruses were present each in one third of the children. adenoviruses, enteroviruses, human meta ... | 2011 | 22007241 |
| human rhinovirus species associated with hospitalizations for acute respiratory illness in young us children. | the contribution of human rhinovirus (hrv) to severe acute respiratory illness (ari) is unclear. | 2011 | 22013207 |
| Human bocavirus and other respiratory viral infections in a 2-year cohort of hospitalized children. | Human bocavirus (HBoV) infection is reported worldwide and may cause severe respiratory tract infections. The aim of the present study was to assess the prevalence of HBoV, and other respiratory viral pathogens, in a 2-year retrospective study of children admitted to hospital, and to investigate whether viral loads of HBoV DNA were associated with severity of infection. Between April 2007 and March 2009, 891 respiratory samples from 760 children admitted to hospital with acute respiratory tract ... | 2012 | 22028039 |
| [detection for respiratory viruses in shanghai with multiplex pcr from 2009 to 2010]. | to characterize the prevalence and occurrence of respiratory viruses in children in shanghai china. | 2011 | 22043718 |
| genotype variability and clinical features of human metapneumovirus isolated from korean children, 2007 to 2010. | this study was undertaken to determine the genotype variability of human metapneumovirus (hmpv) and its circulation pattern over a 3.5-year period, and to evaluate its clinical characteristics in korean children. we investigated 4599 pediatric patients who were referred for a routine respiratory virus test by rt-pcr. hmpv genotype analyses were performed using a nested pcr-restriction fragment length polymorphism assay. clinical and laboratory data obtained from medical records were reviewed ret ... | 2012 | 22056628 |
| ten years of human metapneumovirus research. | described for the first time in 2001, human metapneumovirus (hmpv) has become one of the main viral pathogens responsible for acute respiratory tract infections in children but also in the elderly and immuno-compromised patients. the pathogen most closely related to hmpv is human respiratory syncytial virus (hrsv), the most common cause of bronchiolitis and pneumonia in young children. hmpv has been classified into two main viral groups a and b and has a seasonal distribution in temperate countr ... | 2011 | 22074934 |
| an outbreak of severe respiratory tract infection due to human metapneumovirus in a long-term care facility for the elderly in oregon. | human metapneumovirus (hmpv) was demonstrated to be responsible for an outbreak of acute respiratory tract infection with high morbidity and mortality among residents of a long-term care facility for the elderly during the late spring-summer in oregon. respiratory virus infections are a common cause of death in the elderly and the burden of human metapneumovirus may be underestimated. this case report stresses the importance of hmpv in causing outbreaks in long-term care facilities for the elder ... | 2011 | 22078146 |
| Replication and pathogenicity of attenuated human metapneumovirus F mutants in severe combined immunodeficiency mice. | This study was to evaluate the replication and pathogenicity of attenuated human metapneumovirus (HMPV) mutants in severe combined immunodeficiency (SCID) mice. SCID mice were intranasally infected with either wild type GFP-rHMPV (WT), or mutant viruses (M1, M2 and M4) with the N-linked glycosylation(s) of the F protein removed. The organs were collected for viral isolation, titration, pulmonary histopathology and mRNA detection by PCR at different time points. WT or mutant viruses were successf ... | 2012 | 22085552 |
| prevalence of human metapneumovirus in adults with acute respiratory tract infection in beijing, china. | to evaluate the prevalence and clinical manifestations of human metapneumovirus (hmpv) in immunocompetent chinese adults with acute respiratory tract infections (artis). | 2012 | 22085814 |
| seroepidemiological study of human metapneumovirus in new delhi, india. | purpose: there are a few seroepidemiological studies reported on human metapneumovirus (hmpv) as hmpv was only discovered in the year 2001. this respiratory virus has been reported to be ubiquitous and associated with respiratory tract infections in all age groups. the present study aimed at determining the prevalence of antibodies to hmpv in children and adults of 1 month to 55 years of age. materials and methods: serum samples from 100 study subjects were tested for hmpv antibody by an in-hous ... | 2011 | 22120795 |
| applications of pcr (real-time and masstag) and enzyme-linked immunosorbent assay in diagnosis of respiratory infections and diarrheal illness among deployed u.s. military personnel during exercise balikatan 2009, philippines. | laboratory-based surveillance for diarrheal and respiratory illness was conducted at the 2009 republic of the philippines-united states balikatan exercise to determine the presence of specific pathogens endemic in the locations where the military exercises were conducted. ten stool and 6 respiratory specimens were obtained from individuals meeting case definitions for diarrhea or respiratory illness. stool specimens were frozen in dry ice and remotely tested using enzyme-linked immunosorbent ass ... | 2011 | 22128641 |
| genetic variability and circulation pattern of human metapneumovirus isolated in italy over five epidemic seasons. | human metapneumovirus (hmpv) is an important aetiological agent of respiratory tract infection (rti) in infants. based on genetic differences, hmpvs are separated into two main groups, a and b, and two subgroups 1 and 2. to better understand the genetic diversity of hmpv, we analyzed 435 bp fragments of the f gene from 49 isolates obtained from a paediatric clinical centre in northern italy from 2005 to 2009. the phylogenetic analysis showed that our hmpv sequences clustered into five main clade ... | 2011 | 22143806 |
| performance on a multipathogen external quality assessment (eqa) panel by different mono- and multiplex nucleic acid amplification tests. | an external quality assessment (eqa) panel consisting of a total of 48 samples in broncho alveolar lavage (bal) or transport medium was prepared in collaboration with quality control for molecular diagnostics (qcmd) (www.qcmd.org). the panel was used to assess the proficiency of the 3 laboratories that would be responsible to examine the 6000 samples to be collected in the grace network of excellence (www.grace-lrti.org). the main objective was to decide on the best performing testing approach f ... | 2011 | 22170925 |
| [Etiology survey on virus of acute respiratory infection in Guangzhou from 2006 to 2009]. | To investigate the pathogens of acute respiratory infection (ARI) in Guangzhou from 2006 to 2009. | 2011 | 22177307 |
| Detection of hMPV antigen by EIA in clinical specimens. | Human Metapneumovirus (hMPV) is one of the latest discovered viruses. It has been classified to Paramyxoviridae family. It is the second viral etiological agent, after RSV, which causes respiratory tract infections (RTI) in children, especially children below 5 years old. It is estimated that 5-25% of RTI in children is due to hMPV. In adults hMPV reinfections are bounded to upper respiratory tract infections. The aim of the study was to establish usefulness of ELISA test in detecting hMPV antig ... | 2011 | 22184941 |
| the prevalance of respiratory viruses among healthcare workers serving pilgrims in makkah during the 2009 influenza a (h1n1) pandemic. | despite the high risk of acquiring respiratory infections, healthcare workers who treat pilgrims at hajj have not been studied in previous research on respiratory diseases during hajj. the objective of this study was to determine the prevalence of different respiratory viruses among healthcare workers who treated pilgrims during hajj 2009, the year of the influenza a h1n1 pandemic. a cross-sectional study was performed just before and after hajj (25-29 november, 2009). nasal and throat swabs wer ... | 2011 | 22197024 |
| Human metapneumovirus strains circulating in Latin America. | The human metapneumovirus (HMPV) is responsible for acute respiratory tract infections in young children, elderly patients, and immunocompromised hosts. In this study, we genetically analyzed the circulating HMPV in Central and South America from July 2008 to June 2009 and characterized the strains present in this region. Samples were collected during an international collaborative influenza like illness surveillance study and then sequenced with specific primers for the HMPV G gene. Our results ... | 2011 | 22200894 |
| Human rhinoviruses in severe respiratory disease in very low birth weight infants. | Objectives: To assess incidence, burden of illness, and risk factors for human rhinoviruses (HRVs) in a cohort of very low birth weight (VLBW) infants. Methods: A 2-year prospective cohort study was conducted among VLBW premature infants in Buenos Aires, Argentina. Infants were enrolled in the NICU from June 1, 2003, to May 31, 2005, and managed monthly and with every acute respiratory illness (ARI) during the first year of life. Nasal wash samples were obtained during every respiratory episode ... | 2012 | 22201153 |
| phylogenetic analysis of human metapneumovirus from new york state patients during february through april 2010. | human metapneumovirus (hmpv) is the second leading cause of lower respiratory infection (lri) in children around the world and has been linked to lri in multiple studies. currently, hmpv is classified into 2 major subtypes (a and b), each with 2 subgroups (1 and 2). | 2012 | 22217900 |
| human metapneumovirus in bronchoalveolar lavage fluid of critically ill patients with suspected pneumonia. | 2012 | 22218810 | |
| human metapneumovirus in the cerebrospinal fluid of a patient with acute encephalitis. | to report, to our knowledge, the first case of detection of human metapneumovirus in the cerebrospinal fluid of a patient during acute encephalitis. | 2012 | 22232205 |
| human metapneumovirus (hmpv) binding and infection are mediated by interactions between the hmpv fusion protein and heparan sulfate. | human metapneumovirus (hmpv) is a major worldwide respiratory pathogen that causes acute upper and lower respiratory tract disease. the mechanism by which this virus recognizes and gains access to its target cell is still largely unknown. in this study, we addressed the initial steps in virus binding and infection and found that the first binding partner for hmpv is heparan sulfate (hs). while wild-type cho-k1 cells are permissive to hmpv infection, mutant cell lines lacking the ability to synth ... | 2012 | 22238303 |
| human metapneumovirus-associated hospital admissions over five consecutive epidemic seasons: evidence for alternating circulation of different genotypes. | human metapneumovirus (hmpv) is a pathogen of the respiratory tract with a worldwide distribution. the purpose of this study was to identify hmpv as the cause of acute respiratory diseases in children admitted at spedali civili, a public hospital in brescia, italy. eight hundred forty-six nasopharyngeal aspirate samples negative for the presence of other common respiratory viruses were tested for the presence of hmpv rna by reverse transcription-polymerase chain reaction. of the 846 samples, 79 ... | 2012 | 22246840 |
| epidemiology of respiratory viral infections in two long-term refugee camps in kenya, 2007-2010. | abstract: background: refugees are at risk for poor outcomes from acute respiratory infections (ari) because of overcrowding, suboptimal living conditions, and malnutrition. we implemented surveillance for respiratory viruses in dadaab and kakuma refugee camps in kenya to characterize their role in the epidemiology of ari among refugees. methods: from 1 september 2007 through 31 august 2010, we obtained nasopharyngeal (np) and oropharyngeal (op) specimens from patients with influenza-like illne ... | 2012 | 22251705 |
| [a gexp based multiplex rt-pcr assay for simultaneous detection of twelve human respiratory viruses]. | a gexp based multiplex rt-pcr assay was developed to simultaneously detect twelve different respiratory viruses types/subtypes including influenza a virus, influenza b virus, influenza a virus sh1n1, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, human rhinovirus, human metapneumovirus, adenovirus, respiratory syncytial virus a, respiratory syncytial virus b and human bocavirus. twelve sets of specific primers were designed based on the conserved sequences of ... | 2011 | 22263264 |
| [genetic variability of matrix (m), small hydrophobic (sh) and attachment (g) proteins of human metapneumovirus circulating in children in beijing from 2006 to 2010]. | human metapneumovirus (hmpv) is associated with acute respiratory tract infections (arti) in all age groups. however, there is limited information of genetic analysis of hmpv circulating in beijing. to learn the characteristics of structural protein genes of human metapneumovirus circulating in children in beijing, sequence analysis of matrix (m), small hydrophobic (sh) and attachment (g) proteins of hmpv from 2006 to 2010 was performed. phylogenetic analysis of nucleotide sequences of 42 full l ... | 2011 | 22263268 |
| viral etiology in hospitalized children with acute lower respiratory tract infection. | this study was performed to investigate the viral etiological agents, age distribution and clinical manifestations of lower respiratory tract infection (lrti) in hospitalized children. the viral etiology and clinical findings in 147 children (1 month to 5 years of age) hospitalized with acute lrti were evaluated. cell culture was used for isolation of influenza viruses and direct fluorescent antibody assay for parainfluenza viruses (pivs), respiratory syncytial virus (rsv) and adenoviruses (advs ... | 2011 | 22272450 |
| rna interference in vitro and in vivo using dsirna targeting the nucleocapsid n mrna of human metapneumovirus. | human metapneumovirus causes respiratory diseases with outcomes that can be severe in children, the immunocompromised, and the elderly. synthetic small interfering rnas (sirnas) that silence targeted genes can be used as therapeutic agents. currently, there is no specific therapy for hmpv. in this study, we designed dicer-substrate sirnas (dsirnas) that target metapneumovirus sequences on the mrnas of the n, p, and l genes. in vitro, six dsirnas were shown to inhibit virus replication using cell ... | 2012 | 22285728 |
| is chronic rhinosinusitis caused by persistent respiratory virus infection? | many chronic rhinosinusitis (crs) patients recall an upper respiratory tract infection as the inciting event of their chronic illness. viral infections have been shown to cause obstruction of the osteomeatal complex, which is likely to be a critical step in the development of crs. there is clear overlap between the pathogenesis of crs and asthma. infections with respiratory viruses in childhood increase the risk of subsequently developing asthma. viral infections in established asthmatics are as ... | 2011 | 22287325 |