Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effects of organic fertilizers on aldicarb soil biodegradation in sugar beet crops. | in the present work, the influences of several organic fertilizer treatment regimens were compared as to their slowing down effect on aldicarb soil metabolism in a sugar beet crop. the organic fertilizers treatment schemes had been repeatedly applied in the past 30 years, according to a 3-year rotation cycle. the following organic fertilizers treatment regimens--which are the main ones used in the agronomy practice--were compared: treatment 1: no organic fertilizer at all; treatment 2: 40 tons c ... | 1993 | 8466293 |
| liquid chromatographic detection of a variety of inexpensive sweeteners added to pure orange juice. | liquid chromatography with pulsed amperometric detection was used to analyze pure orange juice adulterated with a variety of inexpensive sweeteners. this method can be used to detect low levels (5-10%) of high fructose corn syrup (42 and 55), cane sugar hydrolysates (50 and 80%), and beet sugar hydrolysates (chemically or enzymatically prepared). | 2008 | 8471860 |
| the galactose binding lectin from the beet armyworm, spodoptera exigua: distribution and site of synthesis. | the spodoptera exigua galactose binding lectin, extracted from hemolymph by affinity chromatography, was comprised of large molecular weight aggregates (100-700 kda). sds-page of this lectin preparation revealed it to contain 2 subunits of 33.2 and 34.4 kda in equimolar concentrations. these subunits had similar amino acid profiles, possessed identical n-terminal sequences and reacted equally to a bank of antilectin monoclonal antibodies. by staining western blots with various lectin conjugate p ... | 1993 | 8485522 |
| assessment of fermentation in growing pigs given unmolassed sugar-beet pulp: a stoichiometric approach. | in four experiments growing pigs were given a cereal-based diet alone or supplemented with unmolassed sugar-beet pulp (sbp), used as a model substrate for fermentation. the rates of production of methane and gaseous hydrogen were measured and, together with the molar proportions of volatile fatty acids (vfa) in the digesta, used in stoichiometric calculations of fermentation. the resulting estimates were only one-sixth of the observed extent of digestion of sbp. bacteriostatic levels of antibiot ... | 1993 | 8490004 |
| structural requirements for efficient translational frameshifting in the synthesis of the putative viral rna-dependent rna polymerase of potato leafroll virus. | the putative rna-dependent rna polymerase of potato leafroll luteovirus (plrv) is expressed by -1 ribosomal frameshifting in the region where the open reading frames (orf) of proteins 2a and 2b overlap. the signal responsible for efficient frameshift is composed of the slippery site uuuaaau followed by a sequence that has the potential to adopt two alternative folding patterns, either a structure involving a pseudoknot, or a simple stem-loop structure. to investigate the structure requirements f ... | 1993 | 8502558 |
| the effect of supplementary formalin treated soya-bean meal on feed intake, milk yield and live-weight gain of dairy cows fed ensiled fodder beets. | the present experiment compared formalin treated soya-bean meal (2 kg dm) with barley and urea (2.4 kg dm) as supplements for a mixture of ensiled beets and ammonium-treated straw given ad libitum in a cross over experiment with two periods of 6 weeks. furthermore, these diets were compared to a traditional diet with fodder beets and ad libitum feeding with grass silage for a period of 12 weeks. the soya-bean meal increased the daily intake of beet-straw silage significantly from 13.1 to 14.1 kg ... | 1993 | 8517769 |
| polycyclic aromatic hydrocarbon contamination in the italian diet. | the content of total and carcinogenic polycyclic aromatic hydrocarbons (pahs) in italian foods was measured. the highest levels of pahs were found in pizza baked in wood-burning ovens and barbecued beef and pork. relatively high levels were also found in beet greens and squash, apples and bread, fried beef, pork and rabbit, cured meats and chocolate. conversely low levels were detected in potatoes and cooked fish, beverages and eggs. the daily intake of total and carcinogenic pahs also was calcu ... | 1995 | 8522036 |
| synthesis of a full-length infectious cdna clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses. | a full-length cdna of cucurbit aphid-borne yellows virus (cabyv) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage t7 rna polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35s promoter in an agroinfection vector. the biological activity of the cloned cdna was demonstrated by the ability of its in vitro transcript to replicate in protoplasts and of the agroinfection vector to infect agroinoculated plants. virus in th ... | 1995 | 8525610 |
| continuous versus discrete information processing in pattern recognition. | a discrete feature model (dfm) and the fuzzy logical model (flmp) were formulated to predict the distribution of rating judgments in a pattern recognition task. the distinction was between the spoken vowels /i/ and /i/, as in beet and bit. subjects were instructed to rate the vowel on a nine-point scale from /i/ to /i/. two features, the first formant frequency (f1) and the vowel duration, were orthogonally varied: the vowel /i/ has a lower (f1) and a longer duration compared to a somewhat highe ... | 1995 | 8525870 |
| physical and gene organization of mitochondrial dna from the fertile cytoplasm of sugarbeet (beta vulgaris l.). | we have constructed a complete physical map of the mitochondrial genome from the male-fertile cytoplasm of sugarbeet. the entire sequence complexity can be represented on a single circular master chromosome of 358 kb. this master chromosome contains three copies of one recombinationally active repeat sequence, with two copies in direct orientation and the other in inverted orientation. the positions of the rrna genes and of 23 polypeptide genes, determined by filter hybridization, are scattered ... | 1995 | 8529269 |
| a comparison of commercial fibre sources: barley bran, corn bran, orange fibre and sugar beet fibre. | 1995 | 8549532 | |
| production of feruloyl/rho-coumaroyl esterase activity by penicillium expansum, penicillium brevicompactum and aspergillus niger. | extracellular esterase production by penicillium expansum, penicillium brevicompactum and aspergillus niger was determined in both liquid and solid-state culture. methyl ferulate was used as the main carbon source in liquid culture whereas wheat bran and sugar beet pulp were used in solid-state culture. extracted enzyme for each fungus showed activity in the presence of onp butyrate, methyl ferulate, methyl coumarate and two 'natural' feruloylated carbohydrate esters. higher enzyme recoveries we ... | 1995 | 8557619 |
| hemicellulose does not affect iron bioavailability in chicks. | two iron repletion experiments using hemoglobin as a response criterion were conducted to assess effects of hemicelluloses on iron bioavailability to chicks. in experiment 1, iron bioavailability from intact fiber sources was determined by adding tomato pomace (14.6% hemicelluloses), soybean hulls (20.6% hemicelluloses), beet pulp (21.5% hemicelluloses), orchard grass (24.1% hemicelluloses) and corn fiber (55.2% hemicelluloses) to a casein dextrose basal diet providing 0.4-4.1% hemicelluloses to ... | 1996 | 8558316 |
| cdna cloning and sequencing of 54kda fragment of the 75kda readthrough protein gene from beet necrotic yellow vein virus. | with viral rna extracted from purified beet necrotic yellow vein virus (bnyvv) isolated from inner mongolia, the first strand of cdna encoding a 54kda fragment of the 75kda readthrough protein on the rna2 was synthesized by reverse transcription. a double-strand cdna fragment of 1.5 kb was obtained after 30 cycles of pcr amplification. the fragment was ligated into and mapped on pgem-7zf(+). the result of sequence analysis shows that the 54kda readthrough domain is 1509 nucleotides (nt). compare ... | 1995 | 8562847 |
| site directed chromosomal marking of a fluorescent pseudomonad isolated from the phytosphere of sugar beet; stability and potential for marker gene transfer. | a plasmid-free, non-pathogenic, ribosomal rna group 1 fluorescent pseudomonad, pseudomonas fluorescens sbw25, was selected from the microflora of sugar beet (beta vulgaris) and modified to contain constitutively expressed marker genes. by site directed homologous recombination a kx cassette [kanamycin resistance (kanr) and catechol 2,3 dioxygenase (xyle)] and a zy cassette [lactose utilization (laczy, beta-galactosidase, lactose permease)] were introduced at least 1 mbp apart on the 6.6 mbp bact ... | 1995 | 8564013 |
| dietary fiber for cats: in vitro fermentation of selected fiber sources by cat fecal inoculum and in vivo utilization of diets containing selected fiber sources and their blends. | two experiments were conducted to evaluate the addition of single sources and blends of dietary fibers to cat diets. in exp. 1, fermentability of selected fibrous substrates by cat fecal microflora was evaluated. after 24 h of fermentation, om disappearance (omd) and total short-chain fatty acid (scfa) production were greatest (p < .05) for citrus pectin, guar gum, and locust bean gum, whereas solka floc resulted in the least (p < .05) omd and total scfa production. in exp. 2, six diets were for ... | 1995 | 8567470 |
| a bacillus thuringiensis insecticidal crystal protein with a high activity against members of the family noctuidae. | the full characterization of a novel insecticidal crystal protein, named cry9ca1 according to the revised nomenclature for cry proteins, from bacillus thuringiensis serovar tolworthi is reported. the crystal protein has 1,157 amino acids and a molecular mass of 129.8 kda. it has the typical features of the lepidoptera-active crystal proteins such as five conserved sequence blocks. also, it is truncated upon trypsin digestion to a toxic fragment of 68.7 kda by removal of 43 amino acids at the n t ... | 1996 | 8572715 |
| sequence comparison and classification of beet luteovirus isolates. | three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (bwyv) and beet mild yellowing virus (bmyv) from europe, iran and the usa. the first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked to this host range specificity are 2 single base pair changes in a 1,200 nucleotide region of the genome. it is proposed that the european bwyv strains that can be transferred at l ... | 1995 | 8572940 |
| investigation of plant organellar dnas by pulsed-field gel electrophoresis. | mitochondrial (mt) dnas from several higher-plant species (arabidopsis thaliana, beta vulgaris, brassica hirta, chenopodium album, oenothera berteriana, zea mays) were separated by pulsed-field gel electrophoresis (pfge). hybridization of the separated dna with mtdna-specific probes revealed an identical distribution of mtdna sequences in all cases: part of the dna formed a smear of linear molecules migrating into the gel, the rest remained in the well. hybridization signals in the compression z ... | 1995 | 8590487 |
| estrogens in unexpected places: possible implications for researchers and consumers. | estrogenic activity originating in unexpected places was encountered on three occasions during an investigation of whether saccharomyces cerevisiae synthesized estrogens. in each instance, estradiol found in the conditioned yeast culture medium originated from an exogenous source and was not synthesized by the yeast. in the first instance, yeast grown in the laboratory showed a time-dependent increase in estradiol in the conditioned medium. however, the culture medium supplement bacto-peptone wa ... | 1995 | 8593858 |
| beet necrotic yellow vein virus 42 kda triple gene block protein binds nucleic acid in vitro. | the triple gene block (tgb) of beet necrotic yellow vein virus rna 2 is required for cell-to-cell movement of the virus rna. the protein p42 encoded by the 5'-proximal gene of the tgb has consensus sequence motifs characteristic of an atp/gtp-dependent helicase. p42 was over-expressed in escherichia coli and shown to bind both single- and double-stranded rna and dna by northwestern blotting. site-directed mutagenesis located the nucleic acid-binding domain to the n-terminal 24 amino acids of the ... | 1996 | 8609485 |
| oxidized ethyl linoleate induces mucosal hypertrophy of the large intestine and affects cecal fermentation of dietary fiber in rats. | oxidized ethyl linoleate (oel) was prepared by aeration at low temperature. peroxide value (pov, meq/kg lipid) of oel was 1400; the major oxidized compounds were 9-hydroperoxy-cis, trans- and 13-hydroperoxy-trans, cis-octadecadienoate. rats fed fiber-free or sugar-beet fiber (sbf, 100g/kg diet) diets were divided into three groups for each diet, and administered oel (high oel group), oel diluted with ethyl linoleate (low oel group, pov 700) and nonoxidized ethyl linoleate (el group) through gast ... | 1996 | 8613881 |
| beet soil-borne virus rna 3--a further example of the heterogeneity of the gene content of furovirus genomes and of triple gene block-carrying rnas. | the complete nucleotide sequence of rna 3 of the ahlum isolate of beet soil-borne virus (bsbv) was determined from cdnas obtained with immunocaptured virus particles and denatured preparations of dsrna. bsbv rna 3 is unique among the plant virus rnas studied so far in containing apparently only the coding sequences of a triple gene block (tgb). the derived amino acid sequences of the three putative tgb-encoded proteins showed the highest level of sequence similarities with those of the correspon ... | 1996 | 8614988 |
| chemoprevention of lung and skin cancer by beta vulgaris (beet) root extract. | the in vitro inhibitory effect of beta vulgaris (beet) root extract on epstein-barr virus early antigen (ebv-ea) induction using raji cells revealed a high order of activity compared to capsanthin, cranberry, red onion skin and short and long red bell peppers. an in vivo anti-tumor promoting activity evaluation against the mice skin and lung bioassays also revealed a significant tumor inhibitory effect. the combined findings suggest that beetroot ingestion can be one of the useful means to preve ... | 1996 | 8620443 |
| nucleotide sequence analysis of rna-5 of five isolates of beet necrotic yellow vein virus and the identity of a deletion mutant. | the nucleotide sequences of rna-5 from two laboratory isolates (d-5 and d-6) and three field isolates (sh1, s43 and r83) of beet necrotic yellow vein virus (bnyvv) were determined. isolates d-5 and d-6, derived from a d field culture during mechanical inoculation, contained rna-5 of molecular size 1.4 kb and 1.0 kb, respectively. the sequences of d-5 sh1, s43 and r83 were found to be at least 98% identical and from 1342 to 1347 nucleotides in length, excluding the poly(a) tail. each contained a ... | 1996 | 8627244 |
| determination of fruit juice authenticity by capillary gas chromatography with flame ionization detection. | a method using capillary gas chromatography with flame ionization detection was developed to determine the addition of high-fructose syrup and beet or cane invert syrup to apple or orange juice. fingerprint oligosaccharides in these inexpensive sweeteners were not detectable (area < 1000) in pure apple or orange juice. one hundred twenty-three pure apple juice and 60 pure orange juice samples representing growing regions around the world were analyzed. ten samples were intentionally adulterated ... | 2008 | 8634542 |
| analysis of the nucleotide sequence of the treehopper-transmitted geminivirus, tomato pseudo-curly top virus, suggests a recombinant origin. | the genome of tomato pseudo-curly top virus (tpctv), originating from florida, has been cloned and sequenced. tpctv is the only geminivirus identified with a vector specificity which falls outside the cicadellidae (leafhoppers) and aleyrodidae (whiteflies). infectivity of the cloned viral genome was demonstrated by agrobacterium-mediated inoculation of several host species. progeny virus was transmissible by the treehopper vector of tpctv, micrutalis malleifera (fowler). the genome of tpctv show ... | 1996 | 8638404 |
| [dietary fiber and pectin fractions of beta vulgaris var. conditiva]. | the alcohol-insoluble substance (ais) from red beet (beta vulgaris var. conditiva alef.) (3.31% of the edible substance) was extracted sequentially with water, ammonium oxalate, 0.05 n hcl and 0.05 n naoh. accordingly 3.93 g, about 0.8 g, 2.96 g resp. 3.80 g galacturonan/100 g ais were extracted with this procedure. these pectin extracts were purified as cu2 +-salts and fractionated into a water-soluble and a water-insoluble part. the composition of neutral monosaccharide units was estimated in ... | 1996 | 8638431 |
| expression analysis of a sucrose synthase gene from sugar beet (beta vulgaris l.). | to investigate the expression pattern of sucrose synthase, a cdna from tap roots of sugar beet (beta vulgaris l.) was isolated using a heterologous sucrose synthase cdna from potato. the 2762 bp long cdna clone designated sbss 1 encodes for a 822 amino acid polypeptide of a predicted molecular mass of 93.7 kda. the deduced amino acid sequence of sugar beet sucrose synthase has homologies of 65-70% when compared to predicted amino acid sequences of sucrose synthases from other species. rna blot a ... | 1996 | 8639746 |
| vip3a, a novel bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. | a novel vegetative insecticidal gene, vip3a(a), whose gene product shows activity against lepidopteran insect larvae including black cutworm (agrotis ipsilon), fall armyworm (spodoptera frugiperda), beet armyworm (spodoptera exigua), tobacco budworm (heliothis virescens), and corn earworm (helicoverpa zea) has been isolated from bacillus thuringiensis strain ab88. vip3-insecticidal gene homologues have been detected in approximately 15% of bacillus strains analyzed. the sequence of the vip3a(b) ... | 1996 | 8643585 |
| purification and coat protein gene sequence of a montana rmv-like isolate of barley yellow dwarf virus. | a montana barley yellow dwarf virus (bydv) isolate, bydv-rmv-mt, is serologically identical to the new york rmv type isolate (rmv-ny) but differs in aphid transmission phenotype. a purification procedure for bydv-rmv-mt was developed and cdnas encompassing the entire coat protein gene and a portion of the putative polymerase gene of both rmv-mt and rmv-ny were cloned and sequenced. diameters of rmv-mt virions averaged 24.7 nm. average virus yield was 4.2 mg/kg plant tissue. there was 81% sequenc ... | 1996 | 8645094 |
| in vitro fermentation of cellulose, beet pulp, citrus pulp, and citrus pectin using fecal inoculum from cats, dogs, horses, humans, and pigs and ruminal fluid from cattle. | we evaluated the influence of gastrointestinal tract microflora from several species on fiber fermentation characteristics in vitro. selected fibrous substrates (cellulose, beet pulp, citrus pulp, and citrus pectin) were incubated for 6, 12, 24, and 48 h with ruminal fluid from cattle or feces from dogs, cats, pigs, horses, or humans. when data were pooled across all substrates and fermentation times, om disappearance (29.4%) and acetate, propionate, butyrate, and total short-chain fatty acid (s ... | 1995 | 8655439 |
| organization of the 3'-terminal half of beet yellow stunt virus genome and implications for the evolution of closteroviruses. | the 3'-terminal half of the beet yellow stunt virus (bysv) genome 10,545 nt, has been cloned and sequenced. the sequenced portion of the bysv genome encompasses 10 open reading frames (orfs) and 241 nt of the 3' untranslated region. the sequence spans, in the 5' to 3' direction, the c-terminal region of the replication-associated polyprotein gene (orf 1a) which includes the set of motifs typical of helicases (hel), the entire 53-kda polymerase (rdrp) gene (orf 1b), and genes encoding 30-kda (orf ... | 1996 | 8661428 |
| reaction kinetics of the vacuolar h(+)-pumping atpase in beta vulgaris. | vacuolar-type h(+)-atpases (v-atpases) are ubiquitous in eukaryote endomembranes, where they are responsible for lumenal acidification. the ratios of h+ translocated per atp hydrolyzed (which may be important in controlling the activity of these pumps) have previously been found to be variable, noninteger and sensitive to cytosolic as well as lumenal ph. the mechanistic implications of these findings are explored here with reaction kinetics. experimental data for this analysis comprise supra- an ... | 1996 | 8661990 |
| transcriptional and post-translational control of the plant plasma membrane h(+)-atpase by mechanical treatments. | the activity of the plant plasma membrane (pm) h(+)-atpase was studied with fresh, cut or aged tissues of sugar beet (beta vulgaris l.) leaves. the rate of acidification of the medium by tissue samples was strongly stimulated by ageing, but unaffected by cutting. the proton-pumping activity and the specific activity of the vanadate-sensitive atpase of purified pm vesicles prepared from aged tissues were much higher than that of fresh tissues, whereas cutting had no effect. yet, both ageing and c ... | 1996 | 8664320 |
| cloning of a cdna encoding a new ribosome-inactivating protein from beta vulgaris vulgaris (mangold). | by means of a lambda zap ii cdna library constructed from seedings of beta vulgaris vulgaris and immunoscreening, a cdna clone containing a partial sequence of a new ribosome-inactivating protein (rip) was obtained. as confirmed by western blot analysis, this clone produced a rip upon induction with iptg. we called it betavulgin (bvg). the recombinant protein (re-bvg) was somewhat smaller than plant-derived rip (28 versus 30 and 32 kda), but showed the specific n-glycosidase activity on tobacco ... | 1996 | 8666251 |
| purification and characterization of a novel esterase induced by growth of aspergillus niger on sugar-beet pulp. | an inducible esterase has been isolated from a liquid culture of aspergillus niger grown on sugar-beet pulp. the enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. the enzyme was not active on methyl sinapinate, a good substrate for ferulic acid esterase iii, which was purified previously from a. niger [faulds and williamson (1994) microbiology 140, 779-787]. with methyl caffeate as substrate the enzyme had temperat ... | 1996 | 8679110 |
| release of ferulic acid from sugar-beet pulp by using arabinanase, arabinofuranosidase and an esterase from aspergillus niger. | aspergillus niger cinnamoyl esterase (cinnae) is shown to be active towards a wide range of feruloylated oligosaccharides derived from sugar-beet pulp (sbp). the esterase hydrolysed ferulic acid ester-linked to either c-2 of arabinose or c-6 of galactose residues, and demonstrated the highest activity towards the feruloylated arabinose trisaccharide. however, cinnae was able to release only 0.88% of total alkali-extractable ferulic acid from sbp in 24 h when acting alone. to determine whether ce ... | 1996 | 8679111 |
| molecular cloning and structural analysis of cdnas that encode 3 small gtp-binding proteins from sugar beet. | isolation of homologous ras-related genes from sugar beet has never been reported. screening cdna library from beta vulgaris l. hilma resulted in the isolation of 3 ras-homologous clones. two of these genes (rab1bv and rab2bv) belong to the rab/ypt group. the deduced polypeptidic sequences from them show strong homologies to ara3 (93% with rab1bv) of arabidopsis thaliana and rgp2 (97% with rab2bv) of oryza sativa. the third gene (rho1bv) belongs to the rho family. the homology of rho1bv protein ... | 1996 | 8680960 |
| effects of fertilizer on insecticides adsorption and biodegradation in crop soils. | recent organic fertilizer treatments (cow manure, pig slurry, composts, or green manure) simultaneously increase insecticide adsorption onto soil and the insecticide soil persistence, indicating a mechanism of slow release of insecticide into soil by the organic matter. this occurred in sugar beet crops with aldicarb, thiofanox and imidacloprid; also, in leek, cauliflower and brussels sprouts crops with chlorpyrifos and chlorfenvinphos. in contrast, organic fertilizer treatments applied once or ... | 1996 | 8687996 |
| high-resolution mapping of repetitive dna by in situ hybridization: molecular and chromosomal features of prominent dispersed and discretely localized dna families from the wild beet species beta procumbens. | members of three prominent dna families of beta procumbens have been isolated as sau3a repeats. two families consisting of repeats of about 158 bp and 312 bp are organized as satellite dnas (sau3a satellites i and ii), whereas the third family with a repeat length of 202 bp is interspersed throughout the genome. multi-colour flourescence in situ hybridization was used for physical mapping of the dna families, and has shown that these tandemly organized families occur in large heterochromatic and ... | 1996 | 8704122 |
| the physical and genomic organization of microsatellites in sugar beet. | microsatellites, tandem arrays of short (2-5 bp) nucleotide motifs, are present in high numbers in most eukaryotic genomes. we have characterized the physical distribution of microsatellites on chromosomes of sugar beet (beta vulgaris l.). each microsatellite sequence shows a characteristic genomic distribution and motif-dependent dispersion, with site-specific amplification on one to seven pairs of centromeres or intercalary chromosomal regions and weaker, dispersed hybridization along chromoso ... | 1996 | 8710945 |
| rrna distribution during microspore development in anthers of beta vulgaris l. quantitative in situ hybridization analysis. | we related changes in the ultrastructural organization of the nucleoli with the results of quantitative in situ hybridizations to characterize rrna metabolism during the development of microspore mother cells in the sugar beet anther (beta vulgaris l.). in the course of meiotic prophase and early postmeiotic interphase the morphological characteristics of the nucleoli are typical of low or no transcriptional activity and a low rate of rrna processing. however, we found evidence of an apparent in ... | 1996 | 8718677 |
| major fruit and vegetable contributors to the main serum carotenoids in the spanish diet. | to identify the main sources of serum carotenoids (lutein, zeaxanthin, lycopene, beta-cryptoxanthin, alpha-carotene and beta-carotene) among the fruits and vegetables in the spanish diet, to be used in the design of food questionnaires and to provide useful information for epidemiological and comparative studies. | 1996 | 8730612 |
| pesticide appliers, biocides, and birth defects in rural minnesota. | earlier studies by our group suggested the possibility that offspring of pesticide appliers might have increased risks of birth anomalies. to evaluate this hypothesis, 935 births to 34,772 state-licensed, private pesticide appliers in minnesota occurring between 1989 and 1992 were linked to the minnesota state birth registry containing 210,723 live births in this timeframe. the birth defect rate for all birth anomalies was significantly increased in children born to private appliers. specific bi ... | 1996 | 8732949 |
| molecular cloning, immunochemical localization to the vacuole, and expression in transgenic yeast and tobacco of a putative sugar transporter from sugar beet. | several plant genes have been cloned that encode members of the sugar transporter subgroup of the major facilitator superfamily of transporters. here we report the cloning, expression, and membrane localization of one of these porters found in sugar beet (beta vulgaris l.). this clone, cdna-1, codes for a protein with 490 amino acids and an estimated molecular mass of 54 kd. the predicted membrane topology and sequence homology suggest that cdna-1 is a member of the sugar transporter family. rna ... | 1996 | 8742332 |
| detection of added beet sugar in concentrated and single strength fruit juices by deuterium nuclear magnetic resonance (snif-nmr method): collaborative study. | a collaborative study of the site-specific natural isotope fractionation-nuclear magnetic resonance (snif-nmr) method for detecting added beet sugar in fruit juices is reported. this method is complementary to the stable carbon isotope ratio analysis (scira) (aoac official methods 981.09 and 982.21), which can detect sugars derived from plants exhibiting c4 metabolism (corn and sugarcane). it is based on the fact that the deuterium content at specific positions of the sugar molecules is higher i ... | 2013 | 8757451 |
| high resolution analysis of the readthrough domain of beet necrotic yellow vein virus readthrough protein: a kter motif is important for efficient transmission of the virus by polymyxa betae. | the 5'-terminal cistron of beet necrotic yellow vein furovirus rna 2 encodes the 21 kda major viral coat protein and terminates with an amber stop codon which can undergo suppression to give rise to a 75 kda readthrough (rt) protein referred to as p75. p75 is a minor component of virions and the 54 kda rt domain following the coat protein sequence is important both for virus assembly and transmission by the fungal vector polymyxa betae. to better define the regions of the rt domain involved in t ... | 1996 | 8757975 |
| horseradish curly top virus is a distinct subgroup ii geminivirus species with rep and c4 genes derived from a subgroup iii ancestor. | the complete nucleotide sequence (3080 nt) of an infectious dna clone derived from the geminivirus horseradish curly top virus (hrctv) has been determined. the relationship of hrctv to other geminiviruses was examined using dot matrix plots of nucleotide sequence similarities, and by phylogeny of predicted amino acid sequences of individual orfs based upon parsimony or neighbour-joining methods. these analyses indicate that the v1 and v2 virion sense orfs of hrctv are most closely related to, ye ... | 1996 | 8757976 |
| [sugar beet pollen allergy as a rare occupational disease]. | although there are a few isolated reports in the literature suggesting that sugar beet pollen is highly antigenic, hypersensitivity to components of sugar beet is not a common disease. we report a 29-year-old man with a history of atopic dermatitis who developed both contact dermatitis and allergic rhinitis from sugar beet pollen through his job in a seed nursery. | 1996 | 8767663 |
| extraction and aphid transmission of beet western yellows luteovirus from air dried leaf tissue. | a protocol is described for the recovery of viable, aphid transmissible beet western yellows luteovirus from air-dried field material. the advantages of the protocol for long-term and cross-border field sampling are discussed. | 1996 | 8783167 |
| the lactational responses of dairy cows to amount of feed and to the source of carbohydrate energy. | an experiment was designed to test whether responses to variation in plane of nutrition conformed to a linear model or to a diminishing response curve model and to examine the influence of type of energy-yielding nutrient used in the ration on the response. lactating friesian cows (n = 18; mean, 126 dim) were used in a latin square experiment with three 4-wk periods. diets consisted of hay and concentrates 40:60 (wt/wt, dm basis). the concentrates were based on grain, sugar beet pulp, or an equa ... | 1995 | 8786258 |
| some preliminary results on the action of rhamnogalacturonase on rhamnogalacturonan oligosaccharides from beet pulp. | sugar-beet pulp was saponified and then hydrolysed with 0.1 m hcl at 80 degrees c for 72 h, and a rhamnogalacturonan fraction was isolated by ion-exchange chromatography on ag 1x8 resin. four individual oligomers, and a mixture of oligomers with higher degrees of polymerization, were obtained by chromatography on biogel p-4. they all presented the alpha-d-galap-(1[-->2)-alpha-l-rhap-(1-->4)-alpha-d-galap -(1]n-->2)-l-rhap structure (with n > or = 2) the five fractions were submitted to hydrolysi ... | 1995 | 8789335 |
| new antifungal proteins from sugar beet (beta vulgaris l.) showing homology to non-specific lipid transfer proteins. | two novel, nearly identical antifungal proteins, iwf1 and iwf2, were isolated from the intercellular washing fluid (iwf) of sugar beet leaves. the proteins were purified to homogeneity and their amino acid sequences were determined. they are basic, monomeric proteins of 91 amino acid residues, 89 of which are identical. both proteins show strong in vitro antifungal activity against cercospora beticola, the casual agent of leaf spot disease in sugar beet. based on primary sequence homology, inclu ... | 1996 | 8790287 |
| characterization of chitinases able to rescue somatic embryos of the temperature-sensitive carrot variant ts 11. | to characterize the acidic endochitinase ep3, able to rescue somatic embryos of the carrot cell line ts11, the enzyme was purified from the medium of wild-type suspension cultures. peptide sequences, deduced amino acid sequences of corresponding pcr-generated cdna clones, serological relation and biochemical properties showed that there were at least five closely related chitinases, four of which could be identified as class iv ep3 chitinases with an apparent size of 30 kda. two other proteins w ... | 1996 | 8790295 |
| the effects of physical form of feed, carbohydrate source, and inclusion of sodium bicarbonate on the diet selections of sheep. | we proposed an hypothesis that ruminants attempt to select a diet that promotes high levels of feed intake by maintaining optimal ruminal conditions. three tests of the hypothesis considered whether the diet selection of sheep given either a choice of two high energy density (ed) feeds or a choice between a high and a low ed feed is affected by 1) sodium bicarbonate inclusion (nahco3; 1, 2, and 4% [wt/wt]) in the high [ed] feed); 2) dietary carbohydrate source (barley-based, b and sugar beet/ ba ... | 1996 | 8791195 |
| behavior of horses is affected by soy lecithin and corn oil in the diet. | to test the hypothesis that dietary fats may improve tractability of horses, the effects of four total mixed diets on behavior were compared. the control diet (con) contained chopped hay, corn, oats, beet pulp, molasses and a mineral mix; the three test diets contained an additional 10% (by weight) corn oil (co), soy lecithin-corn oil (sl-co), or soy lecithin-soy oil (sl-so). eight horses were fed each diet in random order for four 3-wk periods. behavior was observed during the last week of each ... | 1996 | 8791196 |
| purification and characterization of a 43-kda rotenone-insensitive nadh dehydrogenase from plant mitochondria. | a 43-kda nad(p)h dehydrogenase was purified from red beetroot mitochondria. an antibody against this dehydrogenase was used in conjunction with the membrane-impermeable protein cross-linker 3,3'-dithiobis(sulfosuccinimidylpropionate) to localize the dehydrogenase on the matrix side of the inner membrane. immunoblotting showed that the dehydrogenase was found in mitochondria isolated from several plant species but not from rat livers. antibodies against the purified dehydrogenase partially inhibi ... | 1996 | 8798503 |
| medicinal foodstuff. iii. sugar beet. (1): hypoglycemic oleanolic acid oligoglycosides, betavulgarosides i, ii, iii, and iv, from the root of beta vulgaris l. (chenopodiaceae). | betavulgarosides i, ii, iii, and iv, oleanolic acid oligoglycosides having an unique acidic substituent, were isolated from the root of beta vulgaris l. (sugar beet) together with betavulgarosides vi, vii, and viii. the chemical structures of betavulgarosides i, ii, iii, iv were identified from chemical and physicochemical evidence. betavulgarosides ii, iii, and iv were found to exhibit hypoglycemic activity in an oral glucose tolerance test in rats. | 1996 | 8814952 |
| nutrient digestion by ileal cannulated dogs as affected by dietary fibers with various fermentation characteristics. | we studied the effects of dietary fibers with various fermentation characteristics on nutrient digestion at the distal ileum and in the total tract of dogs. the following high-protein (34%), high-fat (23%) diets were fed: 1) a control treatment (con) with 0% supplemental fiber; 2) beet pulp (bp), 7.5%; 3) low-cellulose mixture (lcm), 2.5% cellulose + 5.0% pectin; 4) high-cellulose mixture (hcm), 5.0% cellulose + 2.5% pectin; or 5) solka floc (sf), 7.5% cellulose. nutrient intakes by fiber-supple ... | 1996 | 8818810 |
| is1327, a new insertion-like element in the pathogenicity-associated plasmid of erwinia herbicola pv. gypsophilae. | the pathogenicity-associated plasmid (ppath) of erwinia herbicola pv. gypsophilae was previously shown to be exclusively present in pathogenic strains and to contain a gene cluster encoding phytohormone biosynthesis. sequence analysis of the dna region located downstream from the cytokinin biosynthetic gene (etz) revealed homology to insertion sequences (is) of the is6 family. southern blot analysis performed on plasmid dna of e. herbicola pv. gypsophilae revealed the presence of six copies of t ... | 1996 | 8820751 |
| clastogenic activity of pure chlorophyll and anticlastogenic effects of equivalent amounts of crude extract of indian spinach leaf and chlorophyllin following dietary supplementation to mice. | dietary consumption of green vegetables has been associated with protection against mutagenic and clastogenic activity of genotoxicants. chlorophyll, being present in all green plants, had earlier been suggested to be the principal factor involved. mice were administered (i) crude aqueous extract of leaf of indian spinach, beta vulgaris l. var.benghalensis hort., and equivalent amounts of (ii) chlorophyll extracted from the leaf; (iii) purified chlorophyll, (iv) chlorophyllin, a sodium-copper de ... | 1996 | 8844993 |
| pharmacology of ca2+ release from red beet microsomes suggests the presence of ryanodine receptor homologs in higher plants. | cyclic adp-ribose (cadpr) is known to release ca2+ from plant vacuoles, implying that this nad+ metabolite may possess a second messenger role in plants. the degree to which the plant cadpr-gated ca2+ release mechanism resembles cadpr action in animals has been evaluated. cadpr-elicited ca2+ release from red beet microsomes was inhibited by 1 mm procaine but insensitive to heparin. furthermore, pre-release of ca2+ from red beet vesicles by either 5 mm caffeine or micromolar levels of ryanodine p ... | 1996 | 8849685 |
| genetic mapping of genes for twelve nuclear-encoded polypeptides associated with the thylakoid membranes in beta vulgaris l. | thylakoid membranes of chloroplasts are composed of approx. 75 polypeptide species. nearly 60% originate in nuclear genes, the remainder in plastid genes. in order to localize representatives of the nuclear-encoded gene complement in a eukaryotic plant genome (sugar beet, beta vulgaris l.), we have investigated the rflp patterns of 21 cdnas from spinach that code for thylakoid proteins or proteins peripherally associated with thylakoid membranes. differences in gene dosage were noted between bot ... | 1996 | 8849689 |
| genome analysis of the 3'-terminal part of the little cherry disease associated dsrna reveals a monopartite clostero-like virus. | the high molecular weight dsrna associated with little cherry disease (lcd) was extracted from infected plant tissue and cloned as cdna. the sequence of the 3' 8337 nucleotides was determined. computer assisted translation of the sequence portion identified six open reading frames potentially encoding proteins (from 5' to 3') with molecular masses of 70 kda, 61 kda, 46 kda, 76 kda, 21 kda and 27 kda respectively. a 3'-terminal non-translated region of 210 nucleotides was present. the 70 kda prot ... | 1996 | 8856025 |
| improved delivery of biocontrol pseudomonas and their antifungal metabolites using alginate polymers. | alginate polymer was evaluated as a carrier for seed inoculation with a genetically modified strain pseudomonas fluorescens f113laczy, which protects sugar-beet against pythium-mediated damping-off. f113laczy survived in alginate beads at 5 log10 cfu/bead or higher counts for 8 weeks of storage, regardless of the conditions of incubation. in plant inoculation experiments, colonisation of the growing area of the root by f113laczy, derived from alginate beads placed in the soil next to the seed or ... | 1996 | 8867631 |
| energy metabolism in relation to physical activity in growing pigs as affected by type of dietary carbohydrate. | the effect of dietary carbohydrate source on physical activity in relation to metabolic rate in pigs was studied. six groups of 12 pigs (50-kg, castrated males) were fed one of two diets: a starch diet or a non-starch polysaccharide (nsp) diet. both diets had a similar calculated net energy content. the starch diet contained 13% tapioca, and the nsp diet contained 17% sugar beet pulp silage. pigs were housed in groups and fed at approximately two times maintenance (approximately 900 kj me.kg-.75 ... | 1996 | 8880425 |
| ionic channels of the sugar beet tonoplast are regulated by a multi-ion single-file permeation mechanism. | ionic channels of the sugar beet tonoplast were studied using the patch-clamp technique. at micromolar concentrations of cytosolic calcium, several (at least four) distinct single-channel current levels were routinely identified. on the basis of channel voltage dependence, kinetic properties and conductance of single openings, the largest channel (103 +/- 2 ps in symmetric 150 mm kcl) corresponds to the slow vacuolar (sv) channel already identified by hedrich and neher (1987). the majority of th ... | 1996 | 8881028 |
| highly sensitive immunoassays for detection of barley stripe mosaic virus and beet necrotic yellow vein virus. | enzyme immunoassays based on the use of monoclonal antibodies (mabs) were developed for the detection of the barley stripe mosaic virus (bsmv) and the beet necrotic yellow vein virus (bnyvv). assays employing conjugates of mabs to horseradish peroxidase (hrp) were compared to systems with biotinylated mabs and streptavidin conjugated either to monomeric hrp or to hrp homopolymers with different polymerisation degrees including those of 20, 40 and 80. in the elisa with streptavidin-polymeric hrp ... | 1996 | 8882650 |
| immunodetection of beet necrotic yellow vein virus rna3-encoded protein in different host plants and tissues. | the protein p25 open reading frame (orf) of beet necrotic yellow vein virus-bnyvv rna3 was cloned into bacterial expression vector downstream of the 5-'terminus part of beta-galactosidase orf and the expressed p25 fusion protein was used to produce an antiserum. the latter was employed to detect the subcellular location of p25 in mechanically inoculated tetragonia expansa, chenopodium quinoa and sugarbeet leaves by western blot assay. the results showed that p25 was present as a soluble protein ... | 1996 | 8886114 |
| nutritional implications of pectins in chicks in relation to esterification and origin of pectins. | an experiment was conducted to examine the effect of feeding high-methylated (hm) and low-methylated (lm) pectin on performance and physiological characteristics in broiler chicks. two levels each (1.5 and 3%) of hm citrus pectin (hmc), lm citrus pectin (lmc), or hm sugar beet pectin (hms) were added to a semi-purified basal diet. the experiment was conducted in battery brooders, and chicks received the diets as pellets from 6 to 27 d of age. the inclusion of lmc or hms in the diet had little or ... | 1996 | 8893300 |
| the strain-specific cis-acting element of beet curly top geminivirus dna replication maps to the directly repeated motif of the ori. | strains of beet curly top geminivirus (bctv) possess distinct cis- and trans-acting replication specificity elements which are not separately interchangeable among strains. analysis of the replication competency of chimeric bctv genomes, in which portions of the origin of dna replication (ori) were derived from heterologous bctv strains, have permitted identification of an essential cis-acting element governing strain-specific replication in a subgroup ii geminivirus. our studies indicate that t ... | 1996 | 8941329 |
| beet pseudo-yellows virus is an authentic closterovirus. | we have previously used rt-pcr to amplify a 251 base pair fragment diagnostic for beet pseudo-yellows virus (bpyv) infection from several bpyv infected samples from europe and the usa. this has now been shown by sequencing to represent a fragment of a heat shock protein (hsp)70 gene. sequence analysis of the fragment revealed that it is closely similar to the c-terminal sequence of the hsp70 homologue gene of lettuce infectious yellows virus, a definitive closterovirus, and also has obvious sequ ... | 1996 | 8972572 |
| complete nucleotide sequence of the japanese isolate s of beet necrotic yellow vein virus rna and comparison with european isolates. | the complete nucleotide sequences of beet necrotic yellow vein virus rna-1 to rna-4 of the japanese isolate s (bnyvv-s) were determined and compared with those of french isolate (bnyvv-f2). the nucleotide sequences of the two isolates were very similar, differing by only 1.7% (rna-1), 4.1% (rna-2), 2.9% (rna-3) and 3.6% (rna-4), respectively. the differences of the amino acid sequences of the two isolates depended upon the open reading frames (orf) as follows: p237, 1.4%; p22 (coat protein), 2.1 ... | 1996 | 8973531 |
| a novel chloride channel in vicia faba guard cell vacuoles activated by the serine/threonine kinase, cdpk. | calcium-dependent protein kinases (cdpks) in higher plants contain a c-terminal calmodulin-like regulatory domain. little is known regarding physiological cdpk targets. both kinase activity and multiple ca2+-dependent signaling pathways have been implicated in the control of stomatal guard cell movements. to determine whether cdpk or other protein kinases could have a role in guard cell signaling, purified and recombinant kinases were applied to vicia faba guard cell vacuoles during patch-clamp ... | 1996 | 8978683 |
| structure and expression of mitochondrial citrate synthases from higher plants. | mitochondrial citrate synthase (ec 4.1.3.7) represents the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-coa and oxaloacetate, finally yielding citrate and coa. we report here the isolation of cdna clones encoding citrate synthase from nicotiana tabacum, beta vulgaris and populus. nucleotide and deduced amino acid sequences were compared with previously published sequences of mitochondrial citrate synthases from arabidopsis thaliana and potato, as well as wi ... | 1996 | 8979399 |
| salt stress induces an increased expression of v-type h(+)-atpase in mature sugar beet leaves. | in the halotolerant sugar beet co-expression of v-atpase and a vacuolar na+/h(+)-antiporter provides a mechanism for vacuolar salt sequestration. to analyze salt-induced changes in the expression of the vacuolar h(+)-atpase (v-atpase) a partial cdna of the proton-channel forming subunit c was cloned by rt-pcr. southern blot analysis indicated a small gene family. in control plants transcript levels were high in roots and young growing leaves but low in fully expanded leaves. in mature leaves sal ... | 1996 | 8980504 |
| expression of single-chain antibody fragments (scfv) specific for beet necrotic yellow vein virus coat protein or 25 kda protein in escherichia coli and nicotiana benthamiana. | the coding sequences for the variable regions of heavy and light chains of monoclonal antibodies (mabs) to beet necrotic yellow vein virus (bnyvv) coat protein (cp) or the 25 kda nonstructural protein (p25) were cloned into the pcock vector and expressed as single-chain antibody fragments (scfv) in escherichia coli. for expression in higher plants the scfv were targeted either to the secretory pathway by including the sequences encoding the pectate lyase b (pelb) or the phytohemagglutinin (pha) ... | 1996 | 8980548 |
| a synthetic cryic gene, encoding a bacillus thuringiensis delta-endotoxin, confers spodoptera resistance in alfalfa and tobacco. | spodoptera species, representing widespread polyphagous insect pests, are resistant to bacillus thuringiensis delta-endotoxins used thus far as insecticides in transgenic plants. here we describe the chemical synthesis of a cryic gene by a novel template directed ligation-pcr method. this simple and economical method to construct large synthetic genes can be used when routine resynthesis of genes is required. chemically phosphorylated adjacent oligonucleotides of the gene to be synthesized are a ... | 1996 | 8986755 |
| comparison of the influence of dietary fibre sources with different proportions of soluble and insoluble fibre on ca, mg, fe, zn, mn and cu apparent absorption in rats. | the 6 week experiment was performed on growing wistar male rats. in the third and the sixth weeks digestibility trials were performed. the following high fibre preparations were supplemented to the control diet (c): high methoxylated citrus pectin (cr); apple pomace (a), potato fiber "povex" (p) and sugar beet pulp (b). fibre supplementation, except cr, significantly increased ca, mg, fe, mn, zn, cu excretion in faeces and decreased their apparent absorption in both trials. significant correlati ... | 1996 | 8988315 |
| spodoptera exigua multicapsid nucleopolyhedrovirus deletion mutants generated in cell culture lack virulence in vivo. | the baculovirus spodoptera exigua multicapsid nucleopolyhedrovirus (semnpv) has high potential for development as a bio-insecticide for control of the beet armyworm (s. exigua). it is highly infectious for s. exigua larvae and its host range is very narrow. a prerequisite for such application is the possibility of growing this virus in large quantities, e.g. in insect cell lines. it was observed, however, that polyhedra of semnpv plaque-purified in se-ucr1 cells did not cause larval mortality or ... | 1996 | 9000107 |
| characterization of a new antifungal chitin-binding peptide from sugar beet leaves. | the intercellular washing fluid (iwf) from leaves of sugar beet (beta vulgaris l.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen cercospora beticola (sacc.). among these, a potent antifungal peptide, designated iwf4, was identified. the 30-amino-acid residue sequence of iwf4 is rich in cysteines (6) and glycines (7) and has a highly basic isoelectric point. iwf4 shows homology to the chitin-binding (hevein) domain of chitin-binding pr ... | 1997 | 9008390 |
| isotope ratio mass spectrometric method for the on-line determination of oxygen-18 in organic matter. | a method for the on-line determination of oxygen-18, at a naturally occurring level, in organic material is presented. after pyrolysis of the samples to form carbon monoxide, which is performed at 1300 degrees c in a vitreous carbon tube, the pyrolysis products are transported by a stream of helium gas. using an open split, a small part of the effluent is transferred to the ion source of an isotope ratio mass spectrometer. the ratio is obtained from a measurement of the ion current intensities a ... | 1997 | 9008872 |
| positional cloning of a gene for nematode resistance in sugar beet. | the hs1(pro-1) locus confers resistance to the beet cyst nematode (heterodera schachtii schmidt), a major pest in the cultivation of sugar beet (beta vulgaris l.). the hs1(pro-1) gene was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis. expression of the corresponding complementary dna in a susceptible sugar beet conferred resistance to infection with the beet cyst nematode. the native hs1(pro-1) gene, expressed in roots, encodes a 282-amino acid pro ... | 1997 | 9012350 |
| beet soil-borne virus rna 2: similarities and dissimilarities to the coat protein gene-carrying rnas of other furoviruses. | the complete sequence of the 3454 nt of rna 2 of the ahlum isolate of beet soil-borne furovirus (bsbv) has been determined starting with two short stretches of cloned cdna. unknown parts of the sequence were amplified by means of rt-pcr techniques using combinations of specific and random primers. bsbv rna 2 is more similar in its genetic organization to potato mop top virus (pmtv) rna 3 than to any other furoviral rna, although it is more than 1100 nt longer. its 3'-end, unlike that of pmtv rna ... | 1997 | 9018071 |
| imidacloprid insecticide soil metabolism in sugar beet field crops. | 1996 | 9026154 | |
| comparative analysis of the chromosomal and genomic organization of ty1-copia-like retrotransposons in pteridophytes, gymnosperms and angiosperms. | we have investigated the physical distribution of the reverse transcriptase genes of ty1-copia-like retrotransposable elements from 12 plant species belonging to different subdivisions by hybridization in situ on chromosome preparations. ty1-copia-like elements showed different and non-random hybridization patterns. a dispersed distribution throughout most of the chromosomes with reduced hybridization at some regions or with some weak clustering at other regions was found in allium cepa, beta vu ... | 1997 | 9037155 |
| [the mutagenic action of radioactive contaminants of feed and food products on strains ta98 and ta100 of salmonella typhimurium]. | radionuclide-polluted slices of beef (2775 bq/kg), pork (274.8 bq/kg), potato (159.0-444.0 bq/kg), carrot, garden beet, kidney bean and sorrel (44.5-460.0 bq/kg) have been studied for their mutagenic effect on the tester strains ta98 and ta100 of salmonella typhimurium and this index as a rule correlates with the level of the slices pollution. on the other hand the slices of cabbage and samples of rye and wheat (46.0-460.0 bq/kg) as well as fish (2314.0 bq/kg) had no mutagenic effect on these st ... | 1996 | 9044702 |
| the beet yellows closterovirus p65 homologue of hsp70 chaperones has atpase activity associated with its conserved n-terminal domain but does not interact with unfolded protein chains. | the positive-strand rna genome of beet yellows closterovirus (byv) encodes a 65 kda protein (p65) related to the hsp70 family of cell chaperones. the full-sized byv p65, and n- and c-terminal fragments, with (his)6 tails, were overexpressed in bacteria and purified by metal-chelate chromatography. using a polyclonal antiserum raised against the c-terminal fragment of p65, evidence was obtained for expression of the viral protein in planta. purified recombinant p65 and its n-terminal 40 kda fragm ... | 1997 | 9049401 |
| [a new metabolite with fungistatic and bacteriostatic activity, produced by strain l-30 of flavobacterium sp]. | flavocin, an agent with fungistatic and bacteriostatic activities, was isolated from the culture of flavobacterium sp. l-30. the data on the physico-chemical and biological properties of flavocin a, the major active component, are presented. the component was identified with metabolites of the genus flavobacterium and other genera of nonsporulating bacteria. flavocin was highly efficient in the treatment of various farm crops. the antagonistic effect of flavocin was observed under field conditio ... | 1996 | 9054320 |
| a family of differentially amplified repetitive dna sequences in the genus beta reveals genetic variation in beta vulgaris subspecies and cultivars. | members of a highly abundant restriction satellite family have been isolated from the wild beet species beta nana. the satellite dna sequence is characterized by a conserved rsai restriction site and is present in three of four sections of the genus beta, namely nanae, corollinae, and beta. it was not detected in species of the evolutionary old section procumbentes, suggesting its amplification after separation of this section. sequences of eight monomers were aligned revealing a size variation ... | 1997 | 9060397 |
| [feed intake and performance of dairy cows during feeding of fodder beets supplementing grass silage]. | in a feeding experiment 3 x 12 dairy cows (german simmental x red holstein friesian) were used to investigate the influence of different amounts of fodder beets on feed intake, milk yield and milk composition. the fodder beets (variety kyros) were fed in exchange for concentrate. the amounts of fodder beets were either 22 kg cow-1 d-1 or 44 kg cow-1 d-1 or zero (control group). all groups received 4 kg hay cow-1 d-1, grass silage ad libitum, and concentrate corresponding to the milk yield. in or ... | 1996 | 9065309 |
| influence of dietary factors on nitrogen partitioning and composition of urine and feces of fattening pigs. | an experiment was conducted to investigate the influence of dietary factors on the partitioning of nitrogen excretion and on the ph and composition of urine and feces of fattening pigs. sixteen male hybrid pigs of 80 to 90 kg bw were allotted to one of four diets: based on grains, by-products, grains plus tapioca, or grains plus sugar beet pulp. diets were formulated to have similar contents of ne and cp and a similar lysine:ne ratio. diets differed in nonstarch polysaccharide content (nsp) and ... | 1997 | 9078486 |
| plant cell ph-static circuit mediated by fusicoccin-binding proteins. | on sugar beet protoplasts that carry two types of fusicoccin-binding sites, a ph downshift in a physiological range (7.0-6.6) markedly enhanced the efficiency of fusicoccin (fc) binding, mainly owing to increased avidity of low-affinity fc-binding sites. this may allow the fc-binding proteins to act as ph-sensitive modulators of cell activity, for instance, via plasma membrane h+-atpase or potassium channels. | 1997 | 9089279 |
| the effect of replacing dietary beet pulp with wheat treated with sodium hydroxide, ground wheat, or ground corn in lactating cows. | this experiment examined the effect of complete diets composed of 60% grass silage and 40% concentrate based mainly on beet pulp, ground wheat, wheat treated with naoh, or ground corn on milk production and ruminal fermentation of dairy cows. milk production and fat yield were 19.8, 20.7, 20.1, and 21.2 kg/d and 0.71, 0.76, 0.72, and 0.78 kg/d, respectively (18 cows per treatment). cows fed the diet based on ground corn had higher milk production and fat yield, but lower milk protein concentrati ... | 1997 | 9098804 |
| description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora. | a novel replicative origin (oriv) from a conjugative, mercury resistance plasmid (pqbr11, 304 kbp) has been cloned and sequenced. homology to the pqbr11 oriv-containing 3.55 kbp bamhi fragment (pcv1200) was restricted to one of five genetically distinct classes (group i) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. the oriv of pqbr11 was located within a unique sequence of 300 bp which initiated t ... | 1997 | 9103984 |
| a new arabinofuranohydrolase from bifidobacterium adolescentis able to remove arabinosyl residues from double-substituted xylose units in arabinoxylan. | an arabinofuranohydrolase (axh-d3) was purified from a cell-free extract of bifidobacterium adolescentis dsm 20083. the enzyme had a molecular mass of approximately 100 kda as determined by gel filtration. it displayed maximum activity at ph 6 and 30 degrees c. using an arabinoxylan-derived oligosaccharide containing double-substituted xylopyranosyl residues established that the enzyme specifically released terminal arabinofuranosyl residues linked to c-3 of double-substituted xylopyranosyl resi ... | 1997 | 9114514 |
| effect of autoclaving on dietary fibre content of beet root (beta vulgaris). evaluation by different methods. | modifications of dietary fibre in beet root during autoclaving have been evaluated by different methods: acid detergent (raw = 1.45%; processed = 1.17%) and neutral detergent (raw = 2.30%; processed = 2.00%), the enzymatic-gravimetric method of asp (raw = 3.35%; processed = 3.34%), hplc (raw = 1.42%; processed = 1.60%) and 3,5-dimethylphenol (raw = 0.36; processed = 0.28%). the correlation between different methodologies was studied. autoclaving was carried out at 121 degrees c under pressure fo ... | 1996 | 9123972 |
| production of gibberellic acid by aspergillus niger using some food industry wastes. | the production of gibberellic acid by aspergillus niger and the possibility of utilizing food industry waste and residues as the sources of carbon in media were investigated. media prepared from molasses, vinasse, whey, sugar-beet waste and fruit pomace were used and ga3 yields were found in concentrations 310, 273.14, 120, 73, 118.13 mg/l in such media, respectively. it was observed that food industry wastes can be used as cheap sources of carbon for gibberellic acid production by aspergillus n ... | 1996 | 9127484 |
| effects of mutations in the beet western yellows virus readthrough protein on its expression and packaging and on virus accumulation, symptoms, and aphid transmission. | virions of beet western yellows luteovirus contain a major capsid protein (p22.5) and a minor readthrough protein (p74), produced by translational readthrough of the major capsid protein sequence into the neighboring open reading frame, which encodes the readthrough domain (rtd). the rtd contains determinants required for efficient virus accumulation in agroinfected plants and for aphid transmission. the c-terminal halves of the rtd are not well conserved among luteoviruses but the n-terminal ha ... | 1997 | 9143288 |