Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| treatment of parainfluenza virus type 3 bronchiolitis and pneumonia in a cotton rat model using topical antibody and glucocorticosteroid. | treatment of parainfluenza virus type 3 bronchiolitis and pneumonia in the cotton rat using topical igg cleared infectious virus within 24 h but did not reduce pulmonary pathology. treatment with topical triamcinolone acetonide dramatically reduced pathology but increased virus titers >10-fold. combined therapy, however, demonstrated both favorable effects. animals treated 3,4, and 5 days after infection showed a rebound of lung lesions to levels of untreated animals without reappearance of viru ... | 1996 | 8627023 |
| concern about vaccine study. | 1996 | 8641937 | |
| inhibition of human parainfluenza virus-3 replication by interferon and human mxa. | we have investigated the ifn-mediated inhibition of human parainfluenza virus-3 (hpiv-3) replication in cultured human a549 cells. ifn-alpha inhibited the virus yield significantly with concomitant reduction of viral rna accumulation by more than 90%. further studies indicated that the inhibitory action of ifn was at the level of primary transcription of hpiv3 replication. since the ifn-inducible protein, mxa, has been shown to inhibit virus replication in several rna viruses, we examined the ro ... | 1996 | 8661384 |
| the role of interleukin-10 in the inhibition of t-cell proliferation and apoptosis mediated by parainfluenza virus type 3. | we have previously demonstrated that parainfluenza virus type 3 (piv3), a significant respiratory pathogen, can markedly inhibit t-cell function in vitro. we now report that the virus potently induces interleukin-10 (il-10) production by peripheral blood mononuclear cells. the il-10 produced contributes to viral inhibition of t-cell proliferation and protects t cells from piv3-mediated apoptosis. these findings suggest that il-10 is likely to play an important immunoregulatory role in piv3 infec ... | 1996 | 8676520 |
| new neplanocin analogues. 6. synthesis and potent antiviral activity of 6'-homoneplanocin a1. | the design, synthesis, and antiviral activities of 6'-homoneplanocin a (hnpa, 3) and its congeners having nucleobases other than adenine, such as 3-deazaadenine (4), guanine (5), thymine (6), and cytosine (7), were described. starting from the known cyclopentenone derivative 8, the optically active (mesyloxy)cyclopentene derivative 15 was prepared, which was condensed with nucleobases then deprotected to give target compounds 3-7. of these compounds, hnpa showed an antiviral activity spectrum th ... | 1996 | 8691433 |
| peptides from conserved regions of paramyxovirus fusion (f) proteins are potent inhibitors of viral fusion. | the synthetic peptides dp-107 and dp-178 (t-20), derived from separate domains within the human immunodeficiency virus type 1 (hiv-1) transmembrane (tm) protein, gp4l, are stable and potent inhibitors of hiv-1 infection and fusion. using a computer searching strategy (computerized antiviral searching technology, c.a.s.t.) based on the predicted secondary structure of dp-107 and dp-178 (t-20), we have identified conserved heptad repeat domains analogous to the dp-107 and dp-178 regions of hiv-1 g ... | 1996 | 8700906 |
| functional interaction of paramyxovirus glycoproteins: identification of a domain in sendai virus hn which promotes cell fusion. | the cell fusion activity of most paramyxoviruses requires coexpression of a fusion protein (f) and a hemagglutinin-neuraminidase protein (hn) which are derived from the same virus type. to define the domain of the hn protein which interacts with the f protein in a type-specific manner a series of chimeric hn proteins between two different paramyxoviruses, sendai virus (sn) and human parainfluenza virus type 3 (pi3), was constructed and coexpressed with the sn-f protein by using the vaccinia viru ... | 1996 | 8709235 |
| [the incidence of virus respiratory infections in small children in the season 1994-95]. | as previously, by antibody fluorescent technique the etiology of viral respiratory infections was determinated in small children hospitalized because of respiratory disease in the epidemic season 1994-95. data obtained were compared with the results from the preceding season. among a total of 371 patients, virus infection was detected in 170 (45,8%). data for the whole season did not distinctly differ from those obtained previously; however, some differences were found in the occurrence of the i ... | 1996 | 8711158 |
| parainfluenza-3-virus infection enhances allergic sensitization in the guinea-pig. | viral respiratory tract infections have been previously considered to be associated with induction of allergic sensitization. | 1996 | 8735875 |
| the paramyxovirus simian virus 5 hemagglutinin-neuraminidase glycoprotein, but not the fusion glycoprotein, is internalized via coated pits and enters the endocytic pathway. | the hemagglutinin-neuraminidase (hn) and fusion (f) glycoproteins of the paramyxovirus simian virus 5 (sv5) are expressed on the surface of virus-infected cells. although the f protein was found to be expressed stably, the hn protein was internalized from the plasma membrane. hn protein lacks known internalization signals in its cytoplasmic domain that are common to many integral membrane proteins that are internalized via clathrin-coated pits. thus, the cellular pathway of hn protein internaliz ... | 1996 | 8741847 |
| viral etiology and epidemiology of acute lower respiratory tract infections in korean children. | viral etiologic agents of acute lower respiratory tract infections were studied from november, 1990, through april, 1994, in korean children. from 712 children who visited or were admitted to seoul national university children's hospital because of acute lower respiratory tract infections, 804 nasal aspirates were collected; viral agents were detected by virus isolation and virus antigen was detected by indirect immunofluorescent staining. one or more viral agents were identified in 369 (45.9%) ... | 1995 | 8745017 |
| semi-permissive replication and functional aspects of the immune response in a cotton rat model of human parainfluenza virus type 3 infection. | a cotton rat (sigmodon fulviventer) model of human parainfluenza virus type 3 (hpiv-3) infection was used to study patterns of hpiv-3 replication in naive and immune hosts. growth curves revealed that nasal and pulmonary tissues of naive animals were semi-permissive for virus replication, with amounts of progeny virus proportional to inoculating doses. in naive animals there was a total eclipse in nasal tissues beginning 4 h after inoculation. by contrast, there was only partial eclipse of virus ... | 1996 | 8760420 |
| down-regulation of paramyxovirus hemagglutinin-neuraminidase glycoprotein surface expression by a mutant fusion protein containing a retention signal for the endoplasmic reticulum. | the human parainfluenza virus type 3 (hpiv3) fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins are the principal components involved in virion receptor binding, membrane penetration, and ultimately, syncytium formation. while the requirement for both f and hn in this process has been determined from recombinant expression studies, stable physical association of these proteins in coimmunoprecipitation studies has not been observed. in addition, coexpression of other heterologous param ... | 1996 | 8764007 |
| serological responses in calves to vaccines against bovine respiratory syncytial, infectious bovine rhinotracheitis, bovine viral diarrhoea and parainfluenza-3 viruses. | the istituto superiore di sanità (iss), the national veterinary services laboratory in italy, is in charge of assessing the quality, safety and efficacy of veterinary vaccines before and after licensing. to evaluate the relative potency of several vaccines against bovine respiratory syncytial virus (brsv), infectious bovine rhinotracheitis virus (ibrv), bovine viral diarrhoea virus (bvdv) and parainfluenza-3 virus (pi3v), the serological responses in vaccinated calves were studied. vaccination w ... | 1996 | 8785944 |
| specific interaction in vitro and in vivo of glyceraldehyde-3-phosphate dehydrogenase and la protein with cis-acting rnas of human parainfluenza virus type 3. | human parainfluenza virus type 3 (hpiv3) genome rna is transcribed and replicated by the virus-encoded rna-dependent rna polymerase, and specific cellular proteins play a regulatory role in these processes. to search for cellular proteins potentially interacting with hpiv3 cis-acting regulatory rnas, a gel mobility shift assay was used. two cellular proteins specifically interacted with the viral cis-acting rnas containing the genomic 3'-noncoding region and the plus-sense leader sequence region ... | 1996 | 8798741 |
| new neplanocin analogues. 7. synthesis and antiviral activity of 2-halo derivatives of neplanocin a. | the syntheses and the antiviral activities of 2-halo derivatives of neplanocin a (1b,c), (6'r)-6'-c-methylneplanocin a (2b), and dehydroxymethylneplanocin a (3b,c) are described. sn2 reaction of the known cyclopentenyl units 12 and 13 with 2-haloadenines under basic conditions gave the protected carbocyclic nucleosides 14b,c and 15b,c, respectively. starting from the cyclopentenone derivative 5, the optically active tosyloxycyclopentene derivative 11 was prepared, which was similarly condensed w ... | 1996 | 8809173 |
| comparisons of the f and hn gene sequences of different strains of bovine parainfluenza virus type 3: relationship to phenotype and pathogenicity. | the genes for the f and hn glycoprotein of a pathogenic field isolate of bovine parainfluenza virus type 3 (bpiv3) were isolated, converted to cdna, and sequenced using dideoxynucleotides. the resulting nucleotide sequences were converted to protein sequence and were compared to previously sequenced glycoprotein genes with amino acid differences in the glycoproteins of isolates expressing different phenotypes. the hn glycoprotein, involved in the attachment and release of the virus, and the f gl ... | 1996 | 8809388 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 31-1996. an 18-month old immunosuppressed boy with bilateral pulmonary infiltrates. | 1996 | 8813045 | |
| sequence analyses of human parainfluenza virus type 4a and type 4b fusion proteins. | cdnas encoding human parainfluenza virus type 4a and type 4b (hpiv-4a and -4b) fusion (f) proteins were cloned and sequenced. the predicted amino acid sequences of the f proteins had similar characteristic traits to those reported for the f proteins of other paramyxoviruses. they were more closely related to the f proteins of simian virus 5 (sv5), mumps virus (muv), hpiv-2 and newcastle disease virus (ndv) than to the f proteins of hpiv-1, hpiv-3, sendai virus (sv) and measles virus (mv). in add ... | 1995 | 8847531 |
| antiviral screening of british columbian medicinal plants. | one hundred methanolic plant extracts were screened for antiviral activity against seven viruses. twelve extracts were found to have antiviral activity at the non-cytotoxic concentrations tested. the extracts of rosa nutkana and amelanchier alnifolia, both members of the rosaceae, were very active against an enteric coronavirus. a root extract of another member of the rosaceae, potentilla arguta, completely inhibited respiratory syncytial virus. a sambucus racemosa branch tip extract was also ve ... | 1995 | 8847882 |
| evaluation of a live attenuated bovine parainfluenza type 3 vaccine in two- to six-month-old infants. | a safe and effective parainfluenza type 3 (piv-3) virus vaccine is needed to prevent serious piv-3-associated illness in infants younger than 6 months of age. in previous studies a live bovine piv-3 (bpiv-3) vaccine, which was developed to prevent human piv-3 (hpiv-3) disease, was shown to be safe, infectious, immunogenic and phenotypically stable in 6- to 36-month-old infants and children. | 1996 | 8858666 |
| rapid identification of respiratory viruses: impact on isolation practices and transmission among immunocompromised pediatric patients. | to determine whether empiric isolation of patients with acute respiratory virus infection symptoms could be discontinued when preliminary shell vial cultures were negative, and the impact of this approach on hospital resources. | 1996 | 8880230 |
| rescue of sendai virus cdna templates with cdna clones expressing parainfluenza virus type 3 n, p and l proteins. | several years ago, we reported that a sendai virus (sev) defective genome (dih4uv) could be rescued in vivo with human parainfluenza virus type 1 (hpiv1) and bovine piv3 but not by measles virus or vesicular stomatitis virus. it was concluded that the cis-acting rna sequences were conserved within the sev/piv1/piv3 group but that interactions between the polymerase complex (p-l) and the template protein n were unique for each virus. we have re-examined these conclusions using proteins expressed ... | 1996 | 8887479 |
| detection of human viruses using primary cells immortalised by oncogene transfection, in comparison with primary cells and established cell lines. | no single established cell line was found capable of substituting for primary baboon kidney (pbk) or primary rhesus macacque kidney (prk) cells for detection of human viruses. although a panel of cell lines could detect influenza, parainfluenza, and enteroviruses, which are among the most important viruses encountered in routine diagnostic laboratories, the sensitivity of this panel was not as high as that of pbk or prk cells. however, in a promising complementary approach, pbk and prk cells hav ... | 1996 | 8915884 |
| prenatal ultrasonographic diagnosis of fetal hydrocephalus due to infection with parainfluenza virus type 3. | parainfluenza virus type 3 is one of the most common causes of respiratory infection in infants. no complications of pregnancy or fetal anomalies have been reported in association with parainfluenza virus infection. a pregnancy was terminated at 22 weeks' gestation due to ultrasonographic diagnosis of hydrocephalus. pathological examination was consistent with viral encephalitis, ventriculitis and pneumonia. serological investigation demonstrated a significant rise in maternal antibody titers fo ... | 1996 | 8932633 |
| serological study of responses to selected pathogens causing respiratory tract infection in the institutionalized elderly. | in a prospective 2-year study, serological responses to selected pathogens were analyzed in 224 episodes of fever attributable to respiratory tract infection (51.8%) or of unknown source (48.2%) in 131 residents of two long-term-care facilities. a serological response was identified in 45 episodes (20.1%): chlamydia pneumoniae (14 episodes), haemophilus influenzae type b (1), influenza virus type a (14), respiratory syncytial virus (rsv;2), parainfluenza virus type 3 (7), c. pneumoniae and h. in ... | 1996 | 8953065 |
| analysis of human parainfluenza virus 3 receptor binding variants: evidence for the use of a specific sialic acid-containing receptor. | in order for the fusion protein (f) of the human parainfluenza virus type 3 (hpf3) to promote membrane fusion and viral entry, the haemagglutinin-neuraminidase (hn) glycoprotein must interact with its receptor. sialoglycoconjugates are known to be the receptors for the hpf3 hn, however specific attachment factors or receptors for hpf3 have not been identified. in this report we describe the analysis of variants of hpf3 with increased fusion-promoting phenotypes that were selected by treatment wi ... | 1996 | 8965678 |
| association of the parainfluenza virus fusion and hemagglutinin-neuraminidase glycoproteins on cell surfaces. | we previously observed that cell fusion caused by human parainfluenza virus type 2 or type 3 requires the expression of both the fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins from the same virus type, indicating that a type-specific interaction between f and hn is needed for the induction of cell fusion. in the present study we have further investigated the fusion properties of f and hn proteins of parainfluenza virus type 1 (pi1), type 2 (pi2), and type 3 (pi3), sendai virus (sn ... | 1997 | 8985396 |
| development of a replication-deficient recombinant vaccinia virus vaccine effective against parainfluenza virus 3 infection in an animal model. | the highly attenuated, replication-deficient, modified vaccinia virus ankara (mva) was used to express the fusion (f) and/or hemagglutinin-neuraminidase (hn) glycoproteins of parainfluenza virus 3 (piv3). initial recombinant viruses in which the hn gene was regulated by a very strong synthetic earlyllate promoter replicated poorly in permissive chick embryo cells evidently due to toxic levels of the gene product. this result led us to construct and evaluate a modified earlyllate promoter derived ... | 1996 | 8994321 |
| [a case of adult reye's syndrome with favorable outcome despite status epilepticus]. | a case of adult reye's syndrome is described. a previously healthy 17-year-old man developed convulsions 2 days after resolution of an upper respiratory infection with parainfluenza virus type 3. during the preceding infection, he took aspirin. on admission, he was drowsy. there was no focal signs. cranial ct scan was unremarkable. a lumbar puncture revealed an opening pressure of 180 mm h2o; the cerebrospinal fluid was acellular with normal protein level. serum chemistry showed elevated transam ... | 1996 | 9046859 |
| [efficacy of an indirect immunofluorescence technique in the rapid diagnosis of parainfluenza virus type 3 infection]. | 1997 | 9147513 | |
| an infectious clone of human parainfluenza virus type 3. | a full-length clone of the human parainfluenza virus type 3 (hpiv-3) genome (called phpiv-3) was constructed, and recombinant, infectious hpiv-3 was generated by transfecting phpiv-3 and support plasmids encoding the hpiv-3 np, p, and l proteins into hela cells infected with a vaccinia virus recombinant which expresses t7 rna polymerase. t7 rna polymerase promoters on the transfected plasmids direct the synthesis of transcripts encoding the np, p, and l proteins and a full-length, positive-sense ... | 1997 | 9151814 |
| role of sensory neuropeptides in piv-3-infection-induced airway hyperresponsiveness in guinea pigs. | viral respiratory tract infections are known to induce transient airway hyper-responsiveness. the role of the nonadrenergic noncholinergic neuropeptide system on virus-induced airway hyperresponsiveness was studied in the guinea pig. ten guinea pigs were inoculated with parainfluenza 3 virus (piv-3.2 x 10(6) pfu) by nasal route. 16 animals served as untreated controls. viral infection was proven by histological changes and by demonstration of viral antigen using immunohistochemical techniques. f ... | 1997 | 9154673 |
| the bovine parainfluenza virus type-3 (bpiv-3) hemagglutinin/neuraminidase glycoprotein expressed in baculovirus protects calves against experimental bpiv-3 challenge. | despite the availability of numerous vaccine schedules, "shipping fever", an acute bronchopneumonia brought on in part by a complex of bovine respiratory viruses, remains a major source of economic loss in the beef and dairy industries. we are exploring new strategies of bovine vaccine design which we hope may provide more effective and more cost-efficient control of these pathogens. in this report, we examined the possible use of subunit vaccines, using as an example the hemagglutinin/neuramini ... | 1997 | 9178475 |
| experimental infections with some pneumotropic viruses in the mouse. note ii. virological and pathomorphological aspects of experimental associated infections with parainfluenza virus, adenovirus and respiratory syncytial virus in the mouse. | associated infections with long-strain parainfluenza virus type 3, adenovirus type 3 and respiratory syncytial virus were experimentally induced in white mice. they were pointed out by the appearance of homologous serum antibodies, positive if reactions in the pulmonary tissue, and histological, histochemical and histoenzymatic lesions, which were more severe than those of controls infected with single virus of those mentioned above, since they involved wider areas and had a high frequency. the ... | 1995 | 9179966 |
| phosphorylation of canine distemper virus p protein by protein kinase c-zeta and casein kinase ii. | transcription by nonsegmented negative-strand rna viruses is mediated by the viral rna-dependent rna polymerase and transcriptional cofactor p. the p protein is activated by phosphorylation, an event initiated by cellular kinases. the kinase used differs among this group of rna viruses; vesicular stomatitis virus and respiratory syncytial virus utilize casein kinase ii (ckii), whereas human parainfluenza virus type 3 utilizes pkc isoform zeta (pkc-zeta) for activation of its p protein. to identi ... | 1997 | 9185603 |
| prevalence of antibodies against respiratory viruses (parainfluenza virus type 3, respiratory syncytial virus, reovirus and adenovirus) in relation to productivity in syrian awassi sheep. | awassi sheep sera from all the provinces of the syrian arab republic were tested for rsv, p13, reo and adeno viruses (iif and agid tests). rsv was the most prevalent infection with 63.6% of samples seropositive, followed by reo, p13 and adenovirus with seroprevalences of 27.3%, 24% and 8.1% respectively. animals were more frequently infected by rsv alone. mixed infections were also identified but the occurrence was not high. the rsv and reo virus infections occurred more frequently when transhum ... | 1997 | 9203308 |
| viral regulation of cd95 expression and apoptosis in t lymphocytes. | t lymphocyte apoptosis has been observed in vivo in association with viral infections. one mechanism known to mediate t cell apoptosis is the cd95 (fas/apo-1) ag apoptotic pathway. cd95 is a cell surface protein that is expressed on activated t cells and is capable of relaying an intracellular apoptotic signal upon binding with cd95 ligand. cd95-dependent apoptosis has been shown to be involved in the homeostatic control of peripheral t cell numbers; however, the functional significance of the c ... | 1997 | 9233613 |
| minimum protein requirements for transcription and rna replication of a minigenome of human parainfluenza virus type 3 and evaluation of the rule of six. | a reconstituted transcription and rna replication system for human parainfluenza virus type 3 (hpiv3) was developed using components expressed intracellularly from transfected plasmids driven by t7 rna polymerase supplied by a vaccinia virus recombinant. the system is based on a negative-sense analog of hpiv3 genomic rna in which the viral genes were deleted and replaced with that encoding bacterial chloramphenicol acetyl transferase (cat). the n, p, and l proteins expressed from cotransfected p ... | 1997 | 9234948 |
| detection of respiratory syncytial virus, parainfluenzavirus 3, adenovirus and rhinovirus sequences in respiratory tract of infants by polymerase chain reaction and hybridization. | immunofluorescence assay (ifa) of viral antigens in nasal aspirates is largely used for the diagnosis of respiratory syncytial virus (rsv), parainfluenzavirus (piv) type 3 and adenovirus (adv) infections, whilst rhinovirus (rv) are detected by virus isolation technique (vit) only. using the two techniques, ifa and vit, a significant number of specimens remain negative in spite of clinical and epidemiological presumptions of viral infection. | 1997 | 9248656 |
| prevalence of respiratory viruses and mycoplasma pneumoniae in sputum samples from unselected adult patients. | sputum samples from adult patients are routinely used for bacteriological tests, but not for the diagnosis of viral/mycoplasmal infections. we examined 511 sputum samples submitted for bacterial tests from patients at the royal adelaide hospital. each specimen was tested directly (and after six days of cell culture amplification) for antigens to influenza a and b, parainfluenza 1, 2 and 3, adenovirus, respiratory syncytial virus (rsv) and mycoplasma pneumoniae. respiratory viruses or m. pneumoni ... | 1997 | 9271022 |
| recovery of infectious human parainfluenza virus type 3 from cdna. | infectious hpiv3 was produced by the intracellular coexpression of four plasmid-borne cdnas. these separately encoded a complete hpiv3 genome (negative-sense), the hpiv3 nucleocapsid protein n, the phosphoprotein p, and the polymerase protein l. the cdna-encoded hpiv3 genome differed from the js wildtype (wt) strain of hpiv3 used in its construction by seven point mutations: four of these are silent mutations in the hn or l gene coding regions that serve as markers of a cdna-derived virus, two w ... | 1997 | 9281512 |
| effect of heparin on infection of cells by equine arteritis virus. | the number of plaques formed by equine arteritis virus (eav) and aujesky's disease virus (adv) was reduced to 14% and 5% of the untreated control (100%), respectively, by 10 u/ml of heparin, but could not be reduced below to 13 and 4%, respectively, by use of concentration up to 100 u/ml. an inhibitory effect of heparin, at concentration up to 100 u/ml, was not observed on parainfluenza virus 3 (piv-3). heparinase treatment of rk13 cells reduced the number of eav-, as well as adv-induced plaques ... | 1997 | 9300374 |
| effect of heparin on a haemagglutinin of porcine reproductive and respiratory syndrome virus. | heparin inhibited haemagglutination by porcine reproductive and respiratory syndrome virus (prrsv) and by aujesky's disease virus, but failed to inhibit haemagglutination by parainfluenza virus type 3. the minimal inhibitory concentration of heparin required to inhibit 8 ha u of prrsv haemagglutinin ranged from 0.1 to 1 u ml-1. mouse erythrocytes failed to combine with the haemagglutination inhibitory factor of heparin. however, mouse erythrocytes treated with heparinase had greatly reduced aggl ... | 1997 | 9300545 |
| [molecular evolution of paramyxoviruses]. | we reviewed immunological relationships between paramyxoviruses and molecular evolution of paramyxoviruses. (1) paramyxoviruses are divided into two groups by the basis of the immunological relationship, i.e., parainfluenza type 1 virus group and type 2 virus group; (2) according to the np and m proteins sequences, paramyxoviruses are divided into parainfluenza type 1 virus and type 2 virus groups; the former group is composed of hpiv-1, sv, hpiv-3 and bpiv-3, and the latter group consists of hp ... | 1997 | 9301318 |
| elevation of antibodies to cytomegalovirus and other herpes viruses in pulmonary fibrosis. | the aim of this study was to determine whether latent viral infection is associated with idiopathic pulmonary fibrosis (ipf), an interstitial lung disease whose aetiology remains to be elucidated. cytomegalovirus (cmv) immunoglobulin g (igg) and complement fixation (cf), epstein-barr (eb) viral capsid antigen (vca) igg, herpes simplex virus (hsv) igg, adenovirus cf, and parainfluenza 3 virus haemagglutinin inhibition (hi) titres were measured in the serum from patients with pulmonary diseases. t ... | 1997 | 9311499 |
| cytokine- and virus-stimulated airway smooth muscle cells produce il-11 and other il-6-type cytokines. | inflammation and smooth muscle abnormalities coexist in human airway disorders, and inflammation modulates the proliferative and contractile properties of airway smooth muscle cells. to determine whether human airway smooth muscle (hasm) cells have the ability to regulate local inflammatory processes, we characterized the interleukin (il)-6-type cytokine production of hasm cells in vitro. we demonstrated that hasm cells are potent producers of il-11 when stimulated with il-1 or transforming grow ... | 1997 | 9316501 |
| current status of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) vaccine development: memorandum from a joint who/niaid meeting. | a joint who/national institute of allergy and infectious diseases (niaid) meeting on the current status of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) vaccine development was held in bethesda, md, from 30 september to 1 october 1996. the meeting summarized the worldwide impact of rsv and piv3; presented the current status of development of rsv and piv3 vaccines; and examined the applications of recombinant dna technology to the development and characterization of vacc ... | 1997 | 9342889 |
| induction of procoagulant activity in virus infected bovine alveolar macrophages and the effect of lipopolysaccharide. | three viruses known to be associated with the bovine respiratory disease complex were evaluated in vitro for potential impact upon the procoagulant activity (pca) of bovine alveolar macrophages (bam). cultures of bam were inoculated with bovine parainfluenza virus type 3 (pi-3), cytopathic bovine viral diarrhea virus (cpbvdv), non-cytopathic bvdv (ncpbvdv), or bovine herpes virus type 1 (bhv-1) and incubated for several time periods (24, 48, 72, 96 h). bam were then exposed to e. coli lipopolysa ... | 1997 | 9343337 |
| paramyxovirus fusion (f) protein and hemagglutinin-neuraminidase (hn) protein interactions: intracellular retention of f and hn does not affect transport of the homotypic hn or f protein. | to investigate a possible intracellular coassociation of the paramyxovirus simian virus 5 (sv5) and human parainfluenza virus type 3 (hpiv-3) fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins in a living cell, without resorting to chemical crosslinking and antibody coimmunoprecipitation, we tagged the cytoplasmic n-terminus of sv5 hn with a rrrrr motif and hpiv-3 hn with a rrr motif for endoplasmic reticulum (er) retention. in addition, we tagged the cytoplasmic c-terminus of sv5 and ... | 1997 | 9344902 |
| presence of a unique parainfluenza virus 3 strain identified by rt-pcr in visna-maedi virus infected sheep. | the presence in farm sheep of a paramyxovirus closely related to parainfluenza virus type 3 (pi3) from humans or cattle was confirmed using rt-pcr on rna samples from lung cells from slaughtered animals. sequencing and restriction enzyme patterns of the amplified fragment of the f gene confirms the distinctness of the isolate, and suitable pcr primers allow specific detection of the ovine virus. a study of the incidence of ovine pi3 in samples from sheep with or without distinctive histopatholog ... | 1997 | 9355245 |
| interaction of human parainfluenza virus type 3 with the host cell surface. | 1997 | 9380464 | |
| functional chimeric hn glycoproteins derived from newcastle disease virus and human parainfluenza virus-3. | newcastle disease virus (ndv) is primarily a respiratory tract pathogen of birds, particularly chickens, but it occasionally produces infection in man. human parainfluenza virus type 3 (hpiv3) is a common respiratory pathogen, particularly in young children. these two viruses gain entry to host cells via direct fusion between the viral envelope and the cell membrane, mediated by the two surface glycoproteins: the hemagglutinin-neuraminidase (hn) and fusion (f) proteins. promotion of fusion by hn ... | 1997 | 9413532 |
| [comparison between the llc-mk2 and mdck cell lines in the isolation of parainfluenza virus from nasopharyngeal aspirates]. | we report a prospective comparative study of the efficacy of three commercial cell lines in the isolation of the parainfluenza (pi) virus from nasopharyngeal samples. | 1997 | 9424123 |
| prospects for a parainfluenza virus vaccine. | 1997 | 9443313 | |
| outbreak of parainfluenza virus type 3 in an intermediate care neonatal nursery. | of the four serotypes of human parainfluenza virus, parainfluenza type 3 causes the majority of infections in young children and infants. parainfluenza type 3 can occur in newborns, although most are born with neutralizing antibodies. there have been only infrequent reports of parainfluenza type 3 causing nosocomial respiratory infection in the newborn nursery setting. we report an outbreak occurring in the intermediate care nursery (imcn) at st. boniface hospital, winnipeg, canada. | 1998 | 9469395 |
| experimental researches in infections with associated myxoviruses in the mouse. | infections with influenza virus, a/beijing 353/89 (h3n2) strain, to which there were associated parainfluenza virus type 3, 739-2d strain, adenovirus type 3, and respiratory syncytial virus long strain, were experimentally induced in white mice. the experimental models were set up so as to permit the obtaining of an associated infection with three viruses, in which the influenza virus should be inoculated the first, the participation of the others being variable, according to their presence by a ... | 1996 | 9495782 |
| some virological and pathomorphological aspects of the respiratory system in the experimental infection with respiratory syncytial virus associated with influenza virus, parainfluenza virus type 3 and adenovirus in the mouse. | infections with respiratory syncytial virus long strain, associated with influenza virus, a/beijing 353/89 (h3n2) strain, parainfluenza virus type 3, 739-2d strain, and adenovirus type 3, were experimentally induced in white mice, causing histological, histochemical and histoenzymatic lesions at the respiratory system level, the severity of which exceeded the one observed in the controls infected with a single virus. the pathomorphological changes made up an inflammatory, predominantly infiltrat ... | 1996 | 9495783 |
| three amino acid substitutions in the l protein of the human parainfluenza virus type 3 cp45 live attenuated vaccine candidate contribute to its temperature-sensitive and attenuation phenotypes. | studies were initiated to define the genetic basis of the temperature-sensitive (ts), cold adaptation (ca), and attenuation (att) phenotypes of the human parainfluenza virus type 3 (piv3) cp45 live attenuated vaccine candidate. genetic data had previously suggested that the l polymerase protein of cp45, which contains three amino acid substitutions at positions 942, 992, and 1558, contributed to its temperature sensitivity (r. ray, m. s. galinski, b. r. heminway, k. meyer, f. k. newman, and r. b ... | 1998 | 9499025 |
| detection of respiratory syncytial virus a and b and parainfluenzavirus 3 sequences in respiratory tracts of infants by a single pcr with primers targeted to the l-polymerase gene and differential hybridization. | a reverse transcription-pcr and hybridization-enzyme immunoassay (rt-pcr-eia) has been developed to identify the major agents of bronchiolitis in infants: respiratory syncytial viruses a and b (rsva and rsvb) and parainfluenzavirus 3 (piv3). two primer sets (p1-p2 and p1-p3) were selected in a conserved region of the polymerase l gene. in infected cell cultures, this method detected rsva (n = 14), rsvb (n = 13), and piv3 (n = 13), with the exclusion of piv1 (n = 4), piv2 (n = 3), measles virus ( ... | 1998 | 9508315 |
| sendai viruses with altered p, v, and w protein expression. | wild-type sendai virus expresses three proteins containing the n-terminal half of the p protein open reading frame due to mrna editing; a full-length p protein (ca. 70% of the total), a v protein with the n-terminal half fused to a cys-rich zn(2+)-binding domain (ca. 25% of the total), and a w protein representing the n-terminal half alone (ca. 5% of the total). to examine the role of these proteins in the virus life cycle, we have prepared recombinant viruses in which the normal v mrna expresse ... | 1998 | 9514977 |
| involvement of actin microfilaments in the replication of human parainfluenza virus type 3. | several studies indicate that paramyxoviruses require a specific cellular factor(s) for transcription of their genomic rnas. we previously reported that the cellular cytoskeletal protein actin, in its polymeric form, participates in the transcription of human parainfluenza virus type 3 (hpiv3) in vitro. in the present study, we investigated the role of the polymeric form of actin, i.e., the actin microfilaments of the cytoskeletal framework, in the reproduction of hpiv3 in vivo. pulse-chase labe ... | 1998 | 9525582 |
| recovery of a fully viable chimeric human parainfluenza virus (piv) type 3 in which the hemagglutinin-neuraminidase and fusion glycoproteins have been replaced by those of piv type 1. | the recent recovery of human parainfluenza virus type 3 (piv3) from cdna, together with the availability of a promising, highly characterized live attenuated piv3 vaccine virus, suggested a novel strategy for the rapid development of comparable recombinant vaccine viruses for human piv1 and piv2. the strategy, illustrated here for piv1, is to create chimeric viruses in which the two protective antigens, the hemagglutinin-neuraminidase (hn) and fusion (f) envelope glycoproteins, of an attenuated ... | 1998 | 9525616 |
| cluster headache associated with parainfluenza virus, preceded and succeeded by migraine. | a serologically proven case of parainfluenza viral infection was associated with the onset and disappearance of cluster headache. the patient had long-standing migraine that ceased during the cluster headache period and recurred when the latter stopped. possibly, the virus was neurally transmitted to the trigeminal-autonomic system, creating an inflammatory response that transiently precipitated cluster headache and obliterated migraine. | 1998 | 9529770 |
| simultaneous detection and identification of human parainfluenza viruses 1, 2, and 3 from clinical samples by multiplex pcr. | reverse transcription (rt)-pcr assays have been widely described for use in the diagnosis of human parainfluenza viruses (hpivs) and other respiratory virus pathogens. however, these assays are mostly monospecific, requiring separate amplifications for each hpiv type. in the present work, we describe multiplex rt-pcr assays that detect and differentiate hpiv serotypes 1, 2, and 3 in a combined reaction. specifically, a mixture of three pairs of primers to conserved regions of the hemagglutinin-n ... | 1998 | 9574711 |
| effect of heparin on hemagglutination by equine arteritis virus. | heparin inhibited hemagglutination (ha) by equine arteritis virus (eav) as well as did ha by aujeszky's disease virus (adv), but failed to inhibit ha by parainfluenza virus type 3 (piv-3). the minimal concentration of heparin required to inhibit 8 ha u of eav was 0.1 u/ml. in addition, most eav hemagglutinin was retained by heparin acrylic beads, as was adv hemagglutinin, but was not piv-3 hemagglutinin. mouse erythrocytes failed to combine with the ha inhibitory factor of heparin. however, mous ... | 1998 | 9592716 |
| [effects of roxithromycin (rxm) on viruses and cultured cells]. | 1997 | 9597468 | |
| viral aetiology and epidemiology of acute respiratory infections in hospitalized saudi children. | a total of 1429 saudi children of either sex and under 5 years of age who were admitted to king khalid university hospital, riyadh during a three year period (april 1993-march 1996) with complaints suggestive of acute respiratory tract infections (arti) were investigated for viral aetiology of the infection. viruses could be detected in 522 (37 per cent) cases with respiratory syncytial virus (rsv) the most commonly detected (79 per cent) followed by parainfluenza type 3 (8 per cent). detection ... | 1998 | 9604599 |
| rapid simultaneous diagnosis of infections with respiratory syncytial viruses a and b, influenza viruses a and b, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (hexaplex). | a multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (hexaplex; prodesse, milwaukee) was developed and used to rapidly detect and quantitate rna of respiratory syncytial viruses a and b, influenza viruses a and b, and human parainfluenza virus types 1, 2, and 3 in nasal wash specimens in a single test. primers and probes originated from highly conserved regions of each viral genome. six and a half primer pairs were mixed for the simultaneous detecti ... | 1998 | 9636869 |
| molecular epidemiology of two consecutive outbreaks of parainfluenza 3 in a bone marrow transplant unit. | two consecutive nosocomial outbreaks of parainfluenza 3, in which 5 of 15 infected patients died, occurred in an adult bone marrow transplant unit. parainfluenza 3 strain variation was assessed by reverse transcription-pcr sequencing of part of the parainfluenza 3 f gene, including the noncoding region, directly from clinical samples. sequence data from the outbreaks were compared with those from 15 other parainfluenza 3 isolates circulating concurrently in the community; altogether, 13 strains ... | 1998 | 9666007 |
| the immunogenicity and efficacy of intranasally or parenterally administered replication-deficient vaccinia-parainfluenza virus type 3 recombinants in rhesus monkeys. | immunization of rhesus monkeys with modified vaccinia ankara (mva) recombinants expressing the haemagglutinin-neuraminidase (hn) or fusion (f) glycoproteins of human parainfluenza virus type 3 (hpiv3) was compared with an intranasally-administered live, attenuated hpiv3 vaccine candidate, the cp45 derivative of the js strain of wildtype hpiv3. the mva recombinants, when given parenterally (i.m.) or as a parenteral-local (i.m. and i.t.) combination, induced an antibody response comparable to that ... | 1998 | 9682397 |
| epidemiological, viral and serological studies concerning the evolution of some acute respiratory diseases between november 1992 and march 1993 in nine districts of the south-east area of romania. | viral and serological studies led to the conclusion that parainfluenza type 3 and influenza type a(h3n2) viruses were the most implicated in the etiology of acute respiratory diseases (ard), viral pneumonia and clinical influenza in nine districts of the south-east area of romania during the november 1992-march 1993 period. epidemiological survey pointed on the 0-1 year group of age as the most affected by the above mentioned respiratory diseases. | 1993 | 9702251 |
| [membrane microviscosity in cell/virus systems]. | the use of fluorescent probes for the appraisal of microviscosity properties of cell membranes, both inside the lipid double layer and/or near the double layer surface, are today an usual tool in cell biology. in the present paper an attempt was made to establish if there are significant changes in the mechanical properties of the host cell membranes. | 1993 | 9702252 |
| immune responses of infants to infection with respiratory viruses and live attenuated respiratory virus candidate vaccines. | respiratory viruses such as respiratory syncytial virus (rsv), the parainfluenza viruses (piv), and the influenza viruses cause severe lower respiratory tract diseases in infants and children throughout the world. experimental live attenuated vaccines for each of these viruses are being developed for intranasal administration in the first weeks or months of life. a variety of promising rsv, piv-3, and influenza virus vaccine strains have been developed by classical biological methods, evaluated ... | 1998 | 9711783 |
| myopericarditis associated with parainfluenza virus type 3 infection. | 1998 | 9721970 | |
| detection of mycoplasma pneumoniae in the airways of adults with chronic asthma. | infection with mycoplasma pneumoniae has been shown to exacerbate asthma in humans. however, the role of m. pneumoniae in the pathogenesis of chronic asthma has not been defined. eighteen asthmatics with chronic, stable asthma and 11 nonasthmatic control subjects underwent evaluation of the upper and lower airways and serologic analysis to determine the presence of m. pneumoniae, chlamydia pneumoniae, and seven respiratory viruses through culture, enzyme-linked immunoassay (eia) and polymerase c ... | 1998 | 9731038 |
| membrane fusion promoted by increasing surface densities of the paramyxovirus f and hn proteins: comparison of fusion reactions mediated by simian virus 5 f, human parainfluenza virus type 3 f, and influenza virus ha. | the membrane fusion reaction promoted by the paramyxovirus simian virus 5 (sv5) and human parainfluenza virus type 3 (hpiv-3) fusion (f) proteins and hemagglutinin-neuraminidase (hn) proteins was characterized when the surface densities of f and hn were varied. using a quantitative content mixing assay, it was found that the extent of sv5 f-mediated fusion was dependent on the surface density of the sv5 f protein but independent of the density of sv5 hn protein, indicating that hn serves only a ... | 1998 | 9733810 |
| genetic characterization of parainfluenza virus 3 derived from guinea pigs. | to understand the relationship between novel parainfluenza virus 3 (piv-3), which has recently been isolated from the lungs of guinea pigs, and other piv-3 strains, we determined the complete nucleotide sequence of the novel piv-3 (gpv) genome. a comparison of the nucleotide sequence among piv-3s, including bovine piv-3, revealed that gpv is closely related to human piv-3. the results of the phylogenetic analysis clearly showed that gpv is a lineage of human piv-3, suggesting that gpv has probab ... | 1998 | 9764404 |
| direct detection of respiratory syncytial virus, parainfluenza virus, and adenovirus in clinical respiratory specimens by a multiplex reverse transcription-pcr assay. | diagnosis of respiratory virus infections currently involves detection by isolation or antigen detection, which usually identifies only a single suspected agent. to permit identification of more than one respiratory virus in clinical specimens, a rapid detection method involving a single-step, multiplex reverse transcription-pcr (rt-pcr) assay was developed. the assay included five primer sets that amplified the rna of respiratory syncytial virus subtypes a and b, parainfluenza virus types 1, 2, ... | 1998 | 9774555 |
| pulmonary infections associated with systemic capillary leak syndrome attacks in a patient with hypogammaglobulinemia. | systemic capillary leak syndrome (scls) is a rare disorder of unknown etiology, characterized by recurrent hypovolemic shock attacks associated in most cases with a serum monoclonal immunoglobulin. prophylactic therapy is usually disappointing and the outcome is often fatal. we report on a patient with recurrent hypovolemic shocks consistent with the diagnosis of scls associated with severe serum panhypogammaglobulinemia but no detectable monoclonal immunoglobulin or b cell proliferation. attack ... | 1998 | 9803337 |
| serological diagnosis of respiratory viral infections. a five-year study of hospitalised patients. | the results of a five-year study of paired sera from 410 hospitalised patients-mainly children-with respiratory illness are reported. samples were divided into groups based on clinical diagnosis. the data of each group were analysed in relation to patient age (under or over 1 year of age). the percentage of positive serological diagnoses ranged from 29.4% in the respiratory viral illness group to 46.2% in the bronchiolitis group. each group showed a prevalent serological diagnosis. respiratory v ... | 1998 | 9812318 |
| simultaneous multiple viral infections in childhood acute lower respiratory tract infections in southern taiwan. | thirty paediatric patients with acute lower respiratory tract infections (alri) caused by simultaneous multiple viral infections (smv) in a 3-year interval were reviewed. twenty patients were infected with two viruses simultaneously; nine patients with three viruses; and one patient with four viruses. the frequency of individual viruses were: adenovirus, 18 (60 per cent); respiratory syncytial virus, 7 (23 per cent); influenza virus type a, 6 (20 per cent); influenza virus type b, 15 (50 per cen ... | 1998 | 9819497 |
| virological and pathomorphological aspects in experimental infections with some triassociated pneumotropic viruses in the white mouse. | experimental infections were induced in white mice by intranasal administration of parainfluenza virus type 3, 739-2d strain, to which influenza virus a/beijing, 353/89 (h3n2) strain, and respiratory syncytial virus, long strain, were associated. the model was organized so as to obtain a triassociated infection, parainfluenza virus type 3 being inoculated the first and the other two viruses, in the following stages-ii and iii-, alternately. the infections were revealed by the presence of positiv ... | 1997 | 9836325 |
| two nucleotides immediately upstream of the essential a6g3 slippery sequence modulate the pattern of g insertions during sendai virus mrna editing. | editing of paramyxovirus p gene mrnas occurs cotranscriptionally and functions to fuse an alternate downstream open reading frame to the n-terminal half of the p protein. g residues are inserted into a short g run contained within a larger purine run (angn) in this process, by a mechanism whereby the transcribing polymerase stutters (i.e., reads the same template cytosine more than once). although sendai virus (sev) and bovine parainfluenza virus type 3 (bpiv3) are closely related, the g inserti ... | 1999 | 9847338 |
| isolation of a cytopathogenic virus from a case of porcine reproductive and respiratory syndrome (prrs) and its characterization as parainfluenza virus type 2. | from a lung of a fetus of a breeding sow showing prrs-like symptoms a viral agent could be isolated. it was characterized as an enveloped, hemagglutinating rna virus. ultrastructural examination of purified virus revealed paramyxovirus-like pleomorphic virions of approx. 200 nm in diameter. the helical nucleocapsids were about 18 nm in diameter. the virus was found to be antigenically related to simian virus 5 (sv5) a prototype strain of parainfluenza virus type 2, but not to bovine respiratory ... | 1998 | 9856104 |
| impact of rnase l overexpression on viral and cellular growth and death. | the biologic actions of interferons (ifns) are complex and involve multiple biochemical mechanisms, including the 2-5a system, a regulated rna decay pathway. the 2-5a system is implicated in the antipicornavirus activity of ifn and in the control of apoptosis. to further investigate involvement of the 2-5a system in the control of viral and cellular growth and death, human rnase l cdna was stably expressed in murine 3t3 cells from a constitutive cytomegalovirus (cmv) promoter. a clonal cell line ... | 1998 | 9858317 |
| an epidemiological study of postviral olfactory disorder. | postviral olfactory disorder develops after infection with the common cold, and is a relatively severe and prolonged disorder without rhino-sinusitis. this epidemiological study aimed to characterize its clinical pathology and determine the causative virus. postviral olfactory disorder occurs most commonly in middle-aged women and is most prevalent in spring and summer. a comparison of the monthly frequency of the disorder with the incidence of isolation of various viruses suggest that the influ ... | 1998 | 9879419 |
| identification of mutations contributing to the temperature-sensitive, cold-adapted, and attenuation phenotypes of the live-attenuated cold-passage 45 (cp45) human parainfluenza virus 3 candidate vaccine. | the live-attenuated human parainfluenza virus 3 (piv3) cold-passage 45 (cp45) candidate vaccine was shown previously to be safe, immunogenic, and phenotypically stable in seronegative human infants. previous findings indicated that each of the three amino acid substitutions in the l polymerase protein of cp45 independently confers the temperature-sensitive (ts) and attenuation (att) phenotypes but not the cold-adaptation (ca) phenotype (29). cp45 contains 12 additional potentially important poin ... | 1999 | 9882342 |
| human parainfluenza virus type 3 up-regulates major histocompatibility complex class i and ii expression on respiratory epithelial cells: involvement of a stat1- and ciita-independent pathway. | human parainfluenza virus type 3 (hpiv3) infection causes severe damage to the lung epithelium, leading to bronchiolitis, pneumonia, and croup in newborns and infants. cellular immunity that plays a vital role in normal antiviral action appears to be involved, possibly because of inappropriate activation, in the infection-related damage to the lung epithelium. in this study, we investigated the expression of major histocompatibility complex (mhc) class i and ii molecules on human lung epithelial ... | 1999 | 9882346 |
| respiratory viruses in children attending a major referral centre in saudi arabia. | viral respiratory infections are common causes of illness in infants and children. examination of clinical specimens submitted for diagnosis during a 3-year period (august 1993-july 1996) at king faisal specialist hospital and research center (kfsh & rc) in riyadh revealed a wide spectrum of diseases associated with the isolation of five respiratory viruses. severity of disease ranged from mild upper respiratory illness to threatening lower respiratory illnesses including bronchiolitis and pneum ... | 1998 | 9924568 |
| sensitivity comparison for detection of respiratory bovine coronaviruses in nasal samples from feedlot cattle by elisa and isolation with the g clone of hrt-18 cells. | a monoclonal antibody-based capture enzyme-linked immunosorbent assay (elisa) was developed to detect respiratory bovine coronavirus (rbcv) antigens in nasal swabs collected from cattle showing signs of respiratory tract disease following shipping. these samples had been previously tested for rbcv by inoculation of g clone cultures of human rectal tumor cells (hrt-18g) and for bovine herpes virus 1, parainfluenza virus 3, bovine adenovirus, bovine respiratory syncytial virus, and bovine viral di ... | 1999 | 9925206 |
| alternative mechanisms of interaction between homotypic and heterotypic parainfluenza virus hn and f proteins. | cell fusion by human parainfluenza virus (hpiv) type 2 or type 3 requires the coexpression of both the fusion (f) and haemagglutinin-neuraminidase (hn) glycoproteins from the same virus type, indicating that promotion of fusion requires a type-specific interaction between f and hn. in this report we have further investigated the interaction of the ectodomains of the f and hn glycoproteins from hpiv2 and hpiv3. we constructed mutants of the hpiv2 f and hpiv3 f proteins (f'-kdel) lacking a transme ... | 1999 | 9934692 |
| a live attenuated chimeric recombinant parainfluenza virus (piv) encoding the internal proteins of piv type 3 and the surface glycoproteins of piv type 1 induces complete resistance to piv1 challenge and partial resistance to piv3 challenge. | the recovery of wild type and attenuated human parainfluenza type 3 (piv3) recombinant viruses has made possible a new strategy to rapidly generate a live-attenuated vaccine virus fof piv1. we previously replaced the coding sequences for the hemagglutinin-neuraminidase (hn) and fusion (f) proteins of piv3 with those of piv1 in the piv3 antigenomic cdna. this was used to recover a fully-viable, recombinant chimeric piv3-piv1 virus, termed rpiv3-1, which bears the major protective antigens of piv1 ... | 1999 | 10195620 |
| comparison of the immunogenicity and efficacy of a replication-defective vaccinia virus expressing antigens of human parainfluenza virus type 3 (hpiv3) with those of a live attenuated hpiv3 vaccine candidate in rhesus monkeys passively immunized with piv3 antibodies. | two parainfluenza virus type 3 (piv3) vaccine candidates-cp45, a live attenuated derivative of the js wild type (wt), and a replication-defective vaccinia virus recombinant expressing the hemagglutinin-neuraminidase or fusion glycoprotein of human piv3 (modified vaccinia virus ankara [mva]/piv3 recombinants)-were evaluated in rhesus monkeys to determine whether successful immunization could be achieved in the presence of passively transferred piv3 antibodies. the cp45 virus, administered intrana ... | 1999 | 10228053 |
| parainfluenza 3 spread linked to direct contact. | 1980 | 10315896 | |
| functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants. | we constructed replication-competent human adenovirus type 5 (had5) recombinants (had5-hn and had5-f) containing the bovine parainfluenza virus type 3 (bpiv3) hemagglutinin-neuraminidase (hn) or fusion (f) gene under the control of the simian virus 40 (sv40) regulatory sequences. these genes were inserted in the early region 3 (e3) of the had5 genome in the e3 parallel orientation. expression of hn or f in had5-hn- or had5-f-infected cell extracts, respectively, was observed by immunoprecipitati ... | 1998 | 10325535 |
| [an outbreak of parainfluenza 3 virus infection in the elderly in a ward]. | in the d ward of nagoyashi-koseiin geriatric hospital (36-beds), upper respiratory illnesses were recognized in all the inpatients between july and august in 1995, and we studied 7 elderly subjects with parainfluenza 3 infection diagnosed by serology and viral culture. the outbreak of upper respiratory illnesses occurred in the ward during the 17 days from july 21 through august 6, 1996. fifteen of the 18 elderly persons with upper respiratory illnesses were tested by serology; parainfluenza 3 i ... | 1999 | 10356886 |
| the versatility of paramyxovirus rna polymerase stuttering. | paramyxoviruses cotranscriptionally edit their p gene mrnas by expanding the number of gs of a conserved angn run. different viruses insert different distributions of guanylates, e.g., sendai virus inserts a single g, whereas parainfluenza virus type 3 inserts one to six gs. the sequences conserved at the editing site, as well as the experimental evidence, suggest that the insertions occur by a stuttering process, i.e., by pseudotemplated transcription. the number of times the polymerase "stutte ... | 1999 | 10364305 |
| investigation of seroprevalence of respiratory virus infections in an infant population with a multiantigen fluorescence immunoassay using heel-prick blood samples collected on filter paper. | respiratory viruses are an extremely common cause of childhood morbidity. however, the current seroprevalence of viruses in infant populations is difficult to establish because invasive venipuncture may be technically and ethically unacceptable. this prospective study aimed to establish the seroprevalence of respiratory viruses in an infant population by use of a novel multiantigen fluorescence immunoassay against common respiratory viruses, using heel-prick blood samples collected on filter pap ... | 1999 | 10367768 |
| immune responses and protection induced by dna vaccines encoding bovine parainfluenza virus type 3 glycoproteins. | this study was designed to assess the parameters influencing the magnitude and type of immune responses generated to plasmids encoding the hemagglutinin/neuraminidase (hn) and fusion (f) proteins of bovine parainfluenzavirus type 3 (bpiv3). mice immunized with plasmids expressing hn or f under control of the rous sarcoma virus long terminal repeat promoter were primed, but they did not develop measurable immune responses. in contrast, strong humoral and cellular immune responses were induced wit ... | 1999 | 10405354 |