Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the influence of temperature on muscle velocity and sustained performance in swimming carp. | the aim of this study was to evaluate how fish locomote at different muscle temperatures. sarcomere length excursion and muscle shortening velocity, v, were determined from high-speed motion pictures of carp, cyprinus carpio (11-14 cm), swimming steadily at various sustained speeds at 10, 15 and 20 degrees c. in the middle and posterior regions of the carp, sarcomeres of the lateral red muscle underwent cyclical excursions of 0.31 microns, centered around the resting length of 2.06 microns (i.e. ... | 1990 | 2277258 |
| neural pathway for aggressive display in betta splendens: midbrain and hindbrain control of gill-cover erection behavior. | horseradish peroxidase (hrp) was used to identify parts of the presumptive neural pathway for gill cover erection, a behavioral display pattern performed by siamese fighting fish (betta splendens) during aggressive interactions. motor, motor integration and sensory areas were identified in the medulla and mesencephalon. motor neurons of the dilator operculi muscle, the effector muscle for gill cover erection, are located in the lateral and medial parts of the caudal trigeminal motor nucleus. ion ... | 2008 | 2279236 |
| urotensin i and its n-terminal flanking peptide from the flounder, platichthys flesus. | the caudal spinal cord region of teleost fish terminates in a neurosecretory organ, the urophysis. two peptides have been purified to homogeneity from an extract of the urophysis of a teleost fish, the flounder. the primary structure of one peptide, ser-glu-asp-pro-pro-met-ser-ile-asp-leu10-thr-phe-his-met-leu-arg- asn-met-ile- his20-met-ala-lys-met-glu-gly-glu-arg-glu-gln30-ala-gln-ile- asn-arg-asn-leu-leu - asp-glu40-val, indicates identity with urotensin i. by analogy with other urotensins, t ... | 2006 | 2284199 |
| surface location and stage-specificity of differentiation antigens on germ cells in the common carp (cyprinus carpio), as revealed with monoclonal antibodies and immunogold staining. | during development of juvenile and young adult carp (cyprinus carpio, l., teleostei) three differentiation stages were distinguished in the testis: the prespermatogenic, the early spermatogenic and the advanced spermatogenic testis. carp testis tissue of these stages was dissociated by enzymatic digestion and viable testis cells with well preserved morphological features were obtained. the surface location and stage-specificity of differentiation antigens on these germ cells was investigated usi ... | 1990 | 2286535 |
| preparation of antiserum against carp (cyprinus carpio l.) immunoglobulin and its application for immunohistochemistry. | 1990 | 2287143 | |
| the complete amino acid sequence of prolactin from the sea turtle (chelonia mydas). | the complete amino acid sequence of prolactin (prl) from a reptile, the sea turtle (chelonia mydas), was determined for the first time. sequence analysis was performed on fragments obtained from cleavage of intact and performic acid-oxidized hormone with lysyl endopeptidase, staphylococcus aureus protease, and o-iodosobenzoic acid employing manual edman degradation. the sea turtle prl consists of 198 amino acid residues with three disulfide linkages formed between residues 4-11, 58-173, and 190- ... | 1990 | 2289679 |
| population dynamics and interrelationships of some dactylogyrus and gyrodactylus species on cyprinus carpio. | during 1986/87, 922 carps have been examined for ectoparasitic helminths. altogether 6 species of gyrodactylus and 4 species of dactylogyrus were found. the most frequent parasite on the gills was d. extensus; it shows a great tolerance to the water temperature with two peaks of infestation between 2 and 9 degrees c. additionally, relationships between the seasonal dynamics of the invasion cycle of d. extensus and d. anchoratus were recognized. in addition to dactylogyrus 2 species of gyrodactyl ... | 1990 | 2291498 |
| [hormonal stimulation, in vivo, of the ovary of european eel in the yellow stage]. | the immature ovary of yellow eel was stimulated by a long-term treatment with a gonadotropin ii rich extract of carp pituitary. ovarian follicles of treated yellow eels showed an accumulation of lipidic vacuoles in the oocyte cytoplasm and a thickened follicular envelope (in this regard, they resembled the ovarian follicles of previously studied silver eels). the treatment also resulted in a significant but limited increase in the gonadosomatic ratio and mean follicle size (these values were muc ... | 1990 | 2291806 |
| breakdown of the sperm nuclear envelope is a prerequisite for male pronucleus formation: direct evidence from the gynogenetic crucian carp carassius auratus langsdorfii. | the gynogenetic fish, carassius auratus langsdorfii (the ginbuna, a crucian carp), provides an interesting model for the study of the mechanisms controlling male pronucleus formation. when the sperm nucleus of a different subspecies (c. a. cuvieri) is incorporated into the gynogenetic egg, the nuclear envelope of the spermatozoon is not broken down, and the pronucleus fails to develop, although dispersion of the sperm chromatin occurs to some extent within the space limited by the nuclear envelo ... | 1990 | 2295362 |
| conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species. | blood cells from baltic salmon, salmo salar, three-spined stickleback, gasterosteus aculeatus, eel pout, zoarces viviparus, crucian carp, carassius carassius, african catfish, clarias gariepinus, and reedfish, calamoichthys calabaricus, were incubated with tritiated 11 beta-hydroxyandrostenedione (oha) or 11-ketoandrostenedione (oa). in all fish there was conversion of oa to 11-ketotestosterone (ot), indicating that 17 beta-hydroxysteroid dehydrogenase activity was present in the blood cells. on ... | 1990 | 2295425 |
| functional coupling of glycolysis and phosphocreatine utilization in anoxic fish muscle. an in vivo 31p nmr study. | three fish species with different strategies for anoxic survival (goldfish, tilapia, and common carp) were exposed to environmental anoxia (4, 3, and 1 h, respectively). the concentrations of high energy phosphate compounds and inorganic phosphate, besides the intracellular ph in the epaxial muscle were measured during anoxia and recovery by in vivo 31p nmr spectroscopy. the concentration of free adp was calculated from the equilibrium constant of creatine kinase. during anoxia the patterns of p ... | 1990 | 2295625 |
| gaba-ergic and glycinergic pathways in the inner plexiform layer of the goldfish retina. | gaba-ergic and glycinergic circuitry in the inner plexiform layer of the goldfish retina was evaluated by electron microscopic autoradiography of 3h-gaba and 3h-glycine uptake, combined with retrograde horseradish peroxidase (hrp) labeling of ganglion cells. gaba-ergic and glycinergic synapses were found on labeled ganglion cells throughout the inner plexiform layer. this reinforces the idea that physiological evidence of gaba-ergic and glycinergic influence on a variety of ganglion cells in gol ... | 1990 | 2298935 |
| the role of tubificid oligochaetes in the transmission of goussia carpelli. | tubifex tubifex and limnodrilus hoffmeisteri, fed intestinal tissue of carp containing oocysts of goussia carpelli, produced infections in laboratory-reared carp. sporocysts ingested by the tubificids released the sporozoites that were found to be motile in the intestinal contents and incorporated in intestinal cells of the oligochaetes. tubificids remained infective for carp at least up to 57 days postexposure (pe). tubificids were also able to transmit g. carpelli to carp that had recovered fr ... | 1990 | 2299515 |
| inhibition of glutathione reductase by oncomodulin. | evidence for a specific interaction between oncomodulin and glutathione reductase is presented. glutathione reductase (ec 1.6.4.2) isolated from either the bovine intestinal mucosa or the rat liver was bound in a ca2(+)-dependent manner to oncomodulin which was covalently attached to sepharose. in addition, glutathione reductase was able to catalyze the reduction of the disulfide-linked dimer of oncomodulin. the interaction of these proteins could also be indirectly demonstrated by monitoring gl ... | 1990 | 2306116 |
| seasonal changes in serum gonadotropin, testosterone, 11-ketotestosterone, and estradiol-17 beta levels and their relation to tumor burden in gonadal tumor-bearing carp x goldfish hybrids in the great lakes. | the hybrids of carp (cyprinus carpio) and goldfish (carassius auratus) collected from the lower great lakes between 1978 and 1981 exhibited a high frequency of gonadal neoplasms and apparent sterility, conditions which were rare in the parental species. a pronounced hyperplasia of gonadotropic cells in hybrids resulted in the accumulation of large stores of biologically active gonadotropin (gth) in the pituitary gland. serum gth levels were generally higher in hybrids than in carp collected from ... | 1990 | 2307342 |
| in vivo effect of the organophosphorus insecticide trichlorphon on the immune response of carp (cyprinus carpio). i. effect of contamination on antibody production in relation to residue level in organs. | the organophosphorus insecticide trichlorphon was tested at medium (10 and 20 ppm) and high concentrations (20,000 ppm) on antibody production against yersinia ruckeri vaccine. no statistically significant effect was observed on humoral response or on hematocrit. contamination of organs was analyzed by acetylcholinesterase activity. at 20,000 ppm only liver and brain were highly contaminated; spleen and lymphocytes were not significantly contaminated. at 20 ppm no residue was found in organs. | 1990 | 2311566 |
| in vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (cyprinus carpio). ii. effect of high doses of trichlorphon on nonspecific immune response. | the effect of trichlorphon, one of the most widely used organophosphorus insecticides, on the nonspecific immune response in carp (cyprinus carpio) was studied. the effect of 20,000 ppm trichlorphon on the immune response was followed for 3 and 56 days after intoxication. the effect of 10,000 ppm trichlorphon on the nonspecific immune response of carp experimentally infected by pseudomonas alcaligenes and aeromonas punctata was also examined. leucocyte number, phagocytic ability of neutrophils, ... | 1990 | 2311567 |
| light and electron microscopic observations on the swimbladder stages of sphaerospora renicola, a parasite of carp (cyprinus carpio). | the developmental cycle of stages of sphaerospora renicola, proliferating in the walls of the swimbladder in carp fry and responsible for swimbladder inflammation (= sbi stages) is described. the initial stage, a primary cell containing two secondary cells, grows in size while the secondary cells divide and produce two tertiary cells each by endogenous division. after a supposed disintegration of the grown primary cell, the cell triplets are released to repeat the cycle. the cell divisions are a ... | 1990 | 2315282 |
| mitochondrial and peroxisomal fatty acid oxidation in elasmobranchs. | in heart and red muscle of dogfish (squalus acanthias), the maximal activities of the fatty acid catabolizing enzyme carnitine palmitoyltransferase (cpt) are less than 5% the rate in the same tissues of teleosts (carp, cyprinus carpio; trout, salmo gairdneri). cpt activities in these tissues of hagfish (eptatretus stouti) are approximately 10% the rate in teleosts. however, the maximal activities of the beta-oxidation enzyme beta-hydroxyacyl-coa dehydrogenase (hoad) in dogfish red muscle and hea ... | 1990 | 2316720 |
| the effects of pesticides on some biochemical parameters of carp (cyprinus carpio l.). | treatments with copper sulphate (cuso4), paraquat (pq) and methidathion (md) caused tissue damage and stress effects in carp, indicated by the increased lactate dehydrogenase (ldh), glutamic oxaloacetic transaminase (got), and glutamate dehydrogenase (gidh) enzyme activities and elevated blood-sugar levels. copper sulphate, administered together with pq and md, were synergistic in terms of tissue damage and stress effects. the isoenzyme patterns showed organ-specific tissue damage. the administe ... | 2015 | 2322021 |
| profile of metal-binding proteins and heme oxygenase in red carp treated with heavy metals, pesticides and surfactants. | 1990 | 2322691 | |
| skin grafting in gynogenetic common carp (cyprinus carpio, l.). the development of histocompatible clones. | the fate of skin allografts exchanged among heterozygous and homozygous gynogenetic common carp siblings, and among newly developed inbred strains and f1 hybrids, is described. heterozygous gynogenetic offspring were produced by fertilizing eggs with uv-irradiated sperm and by treating the resulting zygote with a cold shock (0 degree c, 45 min). the temperature shock causes retention of the second polar body, which allows the eggs to develop into normal diploid fry. homozygous gynogenetic offspr ... | 1999 | 2326872 |
| purification, characterization, and molecular cloning of gonadotropin subunits of silver carp (hypophthalmichthys molitrix). | the alpha and beta subunit of silver carp gonadotropin (scgth-alpha and scgth-beta) were isolated by high-performance liquid chromatography. heterogeneity of n-terminal amino acid sequence was observed in scgth-alpha but not in scgth-beta. for determining the complete primary structures of scgth-alpha and scgth-beta, their cdnas were cloned. combining the data of n- and c-terminal sequences determined from proteins and the amino acid sequences deduced from cdnas, we infer that scgth-alpha consis ... | 1990 | 2332148 |
| mitogenic activity from trout embryos. | an extract of 21-day rainbow trout embryos stimulated growth of several piscine cell lines in the absence of added serum. established lines from trout (rtg-2 and ste-137), salmon (chse-214), carp (epc), and goldfish (car) and early-passage cells initiated from trout embryos grew in serum-free medium containing the embryo extract. in addition the extract was sufficient for maintaining long-term cultures of chse-214 cells for several months through a minimum of 20 passages (approximately 50 popula ... | 1990 | 2333296 |
| refined crystal structure of calcium-liganded carp parvalbumin 4.25 at 1.5-a resolution. | the crystal structure of carp parvalbumin (pi = 4.25) has been refined by restrained least-squares analysis employing x-ray diffractometer data to 1.5-a resolution. the final residual for 12,653 reflections between 10 and 1.5 a with i(hkl) greater than 2 sigma(i) is 0.215. a total of 74 solvent molecules were included in the least-squares analysis. the root mean square deviation from ideality of bond lengths is 0.024 a. the model has a root mean square difference of 0.59 a from the positions of ... | 1990 | 2334704 |
| electron microscope autoradiographic examination of uptake behavior of lipophilic chemicals into fish gill. | juvenile carp (cyprinus carpio) were exposed to fenvalerate and to an oligomer with molecular weights of 420 and 2,000-50,000 and log po/w values of 6.4 and more than 14, respectively, and uptake behavior into gill tissues was observed by electron microscope autoradiography. it was qualitatively demonstrated that fenvalerate was absorbed into gill tissues and localized in membrane systems of each cell. on the other hand, no absorption was observed for the oligomer, even in the external membrane ... | 1990 | 2338064 |
| dopamine release via protein kinase c activation in the fish retina. | calcium-dependent phospholipid-sensitive protein kinase [protein kinase c (pkc)] was partially purified from the carp (cyprinus carpio) retina through de 52 ion exchange and cellulofine gel filtration chromatography. the phorbol ester 12-o-tetradecanoylphorbol 13-acetate (tpa) activated pkc in the nanomolar range. a major 38-kda protein in the retinal supernatants (105,000 g) was phosphorylated in vitro by pkc during a short period (3 min). other phosphoproteins also appeared during a further pr ... | 1990 | 2338558 |
| gulonolactone oxidase is missing in teleost fish. the direct spectrophotometric assay. | data in the literature imply that some fish species evolved with the capacity to synthesize ascorbic acid. gulonolactone oxidase activity has been reported in kidney and/or liver tissues. however, it is shown here that this microsomal enzyme activity is missing in common carp hepatopancreas and kidney, whereas high activity was confirmed in pigeon kidney, rat liver, bovine liver and amphibian (xenopus) kidney tissues. a new assay using either the whole tissue homogenate or microsomes solubilized ... | 1990 | 2340104 |
| comparative anatomy of the intestine of cyprinus carpio linnaeus 1758 and salmo trutta linnaeus 1758. | the anatomy of carp and trout was compared by means of anatomical dissection and frozen cross-sections. this enabled exact topographical localization and hence also the determination of differences in the position of individual intestinal segments. the elaboration of a suitable nomenclature was considered to be an important working measure for the facilitation of interspecific comparison. | 1990 | 2341080 |
| relationship between serum got of cyprinus carpio and biotic index of diatom in the diagnosis of water quality. | 1990 | 2354259 | |
| functional analysis of elements affecting expression of the beta-actin gene of carp. | regulatory regions of the beta-actin gene of the common carp (cyprinus carpio) have been examined by linking upstream, 5'-flanking sequences and regions of the first intron to a bacterial chloramphenicol acetyltransferase (cat) reporter gene. by analysis of the mrna products and encoded cat activity, we have identified four putative regions that influence expression: (i) a negative regulatory region 2,300 to 1,100 base pairs (bp) ahead of the gene; (ii) a proximal promoter element, containing th ... | 1990 | 2355913 |
| effect of levamisole on the lymphocyte and macrophage activity in carp (cyprinus carpio). | levamisole, an anthelminthic drug which is often used in cattle, is known for its immunomodulatory effect in several species. in the present work we investigated the in vitro effects of different concentrations of levamisole on lymphocyte proliferation with mitogen in carp. at low concentrations, ie 12.5 to 0.1 micrograms/ml, levamisole stimulated proliferation with phytohemagglutinin p (pha-p) and con a, had no effect at medium concentrations and acted as a suppressor at high concentrations, ie ... | 1990 | 2360777 |
| effect of beta-naphthoflavone and mcpa on liver and kidney drug-metabolizing enzymes from the carp, cyprinus carpio. | the effects of beta-naphthoflavone (beta-nf) and a chlorophenoxyacetic acid herbicide (mcpa) on hepatic and renal monooxygenase activities and conjugating enzymes from immature carp (cyprinus carpio) were studied. beta-nf increased hepatic monooxygenase activities but the patterns of differential induction generally obtained in rat liver microsomes with two series of homologous substrates, alkoxycoumarins and alkylresorufins, were not found to be similar in carp liver microsomes. on the other ha ... | 1990 | 2364910 |
| post-translational processing of prepro-urotensin ii. | the primary structure of a teleost prepro-urotensin ii may be deduced from the nucleotide sequence of cloned dna complementary to carp prepro-urotensin ii mrna but the pathway of post-translational processing of the precursor is unknown. in this study, we have isolated four peptides from an extract of flounder urophysis that are derived from prepro-urotensin ii by proteolytic cleavage. the amino acid sequences of the peptides demonstrate that flounder prepro-urotensin ii is cleaved at two monoba ... | 1990 | 2365069 |
| lymphomyeloid cells, susceptibility to erythrodermatitis of carp and bacterial antigens. | pronounced changes occur in the kidneys carp injected with extracellular product (ecp), lipopolysaccharide (lps) of a. salmonicida subsp. salmonicida, freund's complete adjuvant (fca). ecp caused 50-70% decrease in macrophages and neutrophils, whereas blasts and lymphocyte-like cells increased fca-elicited increased percentages of blasts and a left shift of neutrophils after lps injection. after both ecp and fca injections, the density of major leucocyte types and red blood cells (rbc) in the ki ... | 1990 | 2369967 |
| a cell line (clc) of adherent peripheral blood mononuclear leucocytes of normal common carp cyprinus carpio. | 1990 | 2369971 | |
| [clinico-parasitological research in a mixed focus of clonorchiasis and intestinal nematodiasis in hanamnin province (the socialist republic of vietnam)]. | the authors described the clonorchiasis focus in the vietnam province at the seaside delta of the red river where the aforementioned invasion was accompanied with multiple invasions with intestinal nematodes. clonorchiasis infestation rate was 28.4% ascaridiasis, 50.5%, trichocephaliasis, 21.3% ancylostomiasis, 7.0%. clonorchiasis invasion was the result of consumed uncooked carp fish in the salad. it was revealed that the fish was reared in the ponds near the settlements and was fed untreated h ... | 1990 | 2377136 |
| a comparative study of the unfolding thermodynamics of vertebrate metmyoglobins. | differential scanning microcalorimetry (dsc) of horse, rat, opossum, raccoon, carp, and armadillo metmyoglobins at alkaline ph gave data that fit the two-state unfolding model well. monte carlo studies were used to assess the impact of truncating dsc scans on the reliability of the calculated results when aggregation exotherms overlapped the unfolding endotherm at the high-temperature end of the scan. the dsc estimates for the conformational free energy at ph 8 and 298 k are compared to earlier ... | 1990 | 2378866 |
| temperature adaptation of biological membranes: differential homoeoviscous responses in brush-border and basolateral membranes of carp intestinal mucosa. | the effects of temperature acclimation of carp upon the hydrocarbon order of intestinal membranes has been determined. a fractionation technique has been developed for the simultaneous purification of brush-border and basolateral membrane fractions from the intestinal mucosa. the specific activity of alkaline phosphatase in the brush-border fraction was enhanced 6.4-fold over that of the initial homogenate, whilst the (na(+)-k+)-stimulated atpase was enhanced 5.8-fold in the basolateral fraction ... | 1990 | 2378886 |
| brain growth patterns in four european cyprinid fish species (cyprinidae, teleostei): roach (rutilus rutilus), bream (abramis brama), common carp (cyprinus carpio) and sabre carp (pelecus cultratus). | this study compares brain growth in 4 species of cyprinids, each distinctly different in adult brain morphology: roach have generalized brains; bream are characterized by well-developed visual, octavolateralis and gustatory brain regions; common carp show chemosensory (gustatory)-dominated brains, and sabre carp octavolateralis-dominated brains. the growth patterns of 16 regions relative to total brain volume were investigated by computer-aided quantitative histology to illustrate internal brain ... | 1990 | 2379081 |
| interaction between the soma and the axon terminal of horizontal cells in carp retina. | in teleost retina, the receptive fields of horizontal cell axon terminals have a larger space constant than the receptive fields of the horizontal cell somata. generally this difference in receptive field size is attributed to the cell coupling which is assumed to be stronger in the horizontal axon terminal network than in the horizontal cell soma network. the axon terminals are displaced with respect to the cell bodies. in this paper we show, using a simple simulated horizontal cell (hc) networ ... | 1990 | 2392830 |
| electroretinographic b-wave merely reflects the activity of the rod system in the dark-adapted carp retina. | the electroretinogram and responses of intermediate horizontal cells (ihcs) were recorded simultaneously and studied under dark-adapted conditions in intact crucian carp, with the hope of providing an answer to the controversy whether the scotopic b-wave contains contribution from both rods and cones. our observations indicate that the dark-adapted b-wave follows the univariance principle perfectly in that: (1) b-wave forms and shapes of response amplitude vs light intensity relationships did no ... | 1990 | 2392843 |
| quaternary structure and the geminate recombination of carp hemoglobin with methylisocyanide. | the kinetics of geminate recombination were studied for the methylisocyanide derivative of carp hemoglobin. carp hemoglobin is of interest because it has been established that the fully liganded form switches between a high affinity r state at ph 9 and a low affinity t state at ph 6 in the presence of ihp. geminate recombination was observed on both the picosecond and the nanosecond time scales under all conditions; however, only a small variation is observed in the rates and the yields of gemin ... | 1992 | 2393395 |
| gene transfer, expression and inheritance of prsv-rainbow trout-gh cdna in the common carp, cyprinus carpio (linnaeus). | a recombinant plasmid containing the rous sarcoma virus-long terminal repeat (rsv-ltr) promoter linked to rainbow trout (salmo gairdneri) growth hormone (gh) cdna was microinjected into fertilized carp eggs. genomic dna extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and southern blot hybridization, using the rsv-ltr and/or the gh cdna sequences as probes. out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain prsv-rtg ... | 1990 | 2393581 |
| the shift from c-19 to c-21 steroid synthesis in spawning male common carp, cyprinus carpio, is regulated by the inhibition of androgen production by progestogens produced by spermatozoa. | there is a rapid shift in the steroidogenic pathway from androgen to progestogen production in spawning male common carp, cyprinus carpio. experiments were conducted to determine the mechanism regulating this shift using in vitro cultures of testicular fragments and isolated sperm of spermiating male carp. the levels of 11-ketotestosterone (11-kt) continually increased for 48 h with or without gonadotropin (gth) stimulation, suggesting that 11-kt is the principal androgen produced by carp testes ... | 1990 | 2393683 |
| isolation of carp genes encoding major histocompatibility complex antigens. | in the evolution of the adaptive immune system unique to vertebrates, teleost fish occupy the critical position. this is the most primitive class of lower vertebrates in which the capacity for acute allograft rejections can be demonstrated, thus suggesting the presence of major histocompatibility complex (mhc) antigens and, therefore, t cells. here, we report the identification of two putative mhc-antigen-encoding sequences in the carp cyprinus carpio. one, identified as tlai alpha-1, had reason ... | 1990 | 2395879 |
| europium(iii) ion luminescence as a structural probe of parvalbumin isotypes. | the 7f0----5d0 transition of eu3+ is used to investigate the metal ion binding sites of five parvalbumin isotypes. comparisons are based on the resolution of the eu3+ excitation spectrum with a computer program using the marquardt nonlinear regression algorithm. the lorentzian-gaussian product function provides the most effective approximation to the shape of the peaks, the positions of which were found to be related to differences in the amino acid residues at the binding sites. below ph 6, spe ... | 1990 | 2400774 |
| the complete nucleotide sequence of the growth-hormone gene from the common carp (cyprinus carpio). | we have isolated and sequenced a phase clone from a common carp (cyprinus carpio) genomic library that carries a gene encoding growth hormone (gh). this gene consists of five exons and four introns spanning a region of about 3 kilobase pairs. its exons correspond with one of two reported cdnas of carp gh except for nine differences in the nucleotide sequence, while the encoded amino-acid sequences are identical. the sequence upstream from the transcription start point contains two tandem repeats ... | 1990 | 2400791 |
| [the contamination of food trout and carp from bavarian fish farms with radiocesium (cs-137 + 134) 1986 to 1989 as the result of the nuclear power plant accident at chernobyl]. | after the nuclear power station accident of chernobyl at 26. 4. 86 southern bavaria was contaminated with radionuclides as j-131, cs-137 and cs-134. after three months only the cesium nuclides had bearing on food, accordingly for fish. the accumulation of total cesium (137 + 134) in the muscle of trout and carp had reached levels of 30 and 80 bq/kg on an average and levels for 300 and about 800 bq/kg were determined as maximum. the more naturally fed carps had have in the first two years a highe ... | 1990 | 2401195 |
| [problems with malachite green use in fish]. | the arylmethane dye malachite green oxalate is used as an antimycotic and antiparasitic substance for treatment of fish diseases. the therapeutical use of malachite green is not licensed by the german drug act. a decree fixes a limit of tolerance of 0.01 mg malachite green/kg food produced from fish. a so-called standard license will be prepared for malachite green as a drug for fishes. by trout and carp, malachite green is eliminated slowly. therefore a long withdrawal time is necessary. the to ... | 1990 | 2401196 |
| distribution of gaba-like immunoreactivity in myenteric plexus of carp, frog and chicken. | the distribution of gaba-like immunoreactivity was studied by means of indirect immunocytochemical methods in some lower vertebrate species (carp, frog, chicken). an immunoreactive network was revealed in the myenteric plexus of the alimentary canal of carp. gaba-positive nerve cells were attached closely to the fibres in the stomach. in other gut regions immunostained neurons were less frequent. immunoreactive fibres often formed baskets on the surfaces of immunonegative neurons along the whole ... | 1990 | 2401638 |
| [the effect of silver carp oil on the blood pressure and lipids of spontaneously hypertensive rats]. | 1990 | 2402274 | |
| chemical analysis of internal environmental response of carp puntius stigma to ddt. | exposure of puntius stigma to sublethal dose of ddt (0.05 ppm) for 15 days decreased the liver concentrations of protein and dna from 65 +/- 0.91 to 63 +/- 0.78 mg/100 mg and 669 +/- 17 to 575 +/- 14 micrograms/100 mg, respectively whereas rna increased from 8214 +/- 318 to 8929 +/- 209 micrograms/100 mg. biotransformation products of this hydrocarbon interfered in the pathways of protein biosynthesis and induced proteolysis in hepatic tissues. the resulting changes were explained in the light o ... | 1985 | 2416702 |
| electrical properties of on-off transient amacrine cells in the carp retina. | 2008 | 2417175 | |
| the differentiation of germ cells and gonads during development of carp (cyprinus carpio l.). a study with anti-carp sperm monoclonal antibodies. | gonadal development, germ cell differentiation and the appearance of membrane antigenic determinants, specific for male and female germ cells during gonadogenesis, was studied in larval and juvenile carp (cyprinus carpio l.) until 25 weeks after fertilization. indirect immunofluorescence studies with four monoclonal antibodies raised against carp spermatozoa revealed that monoclonal antibody wcs 29 stained the outer membranes of primordial germ cells in larvae from 3 days after fertilization. th ... | 1985 | 2422310 |
| cloning and sequence analysis of cdnas encoding precursors of urotensin ii-alpha and -gamma. | the primary structures of the precursors of urotensin ii (uii)-alpha and -gamma, neuropeptide hormones of the caudal neurosecretory system of the carp, cyprinus carpio, have been determined by analyzing the nucleotide sequences of cloned dnas complementary to the mrnas encoding them. a cdna library, constructed with poly(a)+rna in the preterminal spinal cord, was screened using 32p-labeled synthetic oligonucleotides representing all possible cdna sequences corresponding to the pentapeptide commo ... | 1986 | 2427672 |
| karyotypes of cyprinus carpio and leuciscus cephalus. | karyological investigations on the common carp (cyprinus carpio) and chub (leuciscus cephalus) are described. the diploid chromosome complement in the carp was found to be 98, with 50 meta- to submetacentric chromosomes, 48 subtelo- to telocentric chromosomes, and an arm number of 148. chromosomal studies on the chub revealed the diploid chromosome complement to be 50, with 34 meta- to submetacentric chromosomes, 16 subtelo- to telocentric chromosomes, and an arm number of 84. | 1986 | 2428561 |
| effects of direct immersion in antigen on immunological memory in young carp, cyprinus carpio. | the study asks whether, in fish, antigens encountered early in life can prime the immune system to yield memory responses on subsequent challenge with the same antigen and, if so, whether positive immunity or immunological tolerance is induced. the direct immersion method of vaccination was used to prime 4 week old carp, cyprinus carpio, and was compared with priming by injection. three different forms of antigen were used: the thymus dependent antigen, human gamma globulin (hgg) in soluble and ... | 1986 | 2429432 |
| telencephalic afferent nuclei in the carp diencephalon, with special reference to fiber connections of the nucleus preglomerulosus pars lateralis. | fiber connections of the nucleus preglomerulosus pars lateralis (pgl), which primarily provides afferent inputs to the telencephalon, were examined in carp by means of horseradish peroxidase tracing methods. the major afferent sources of pgl are the bilateral nucleus tuberis anterior and a few projections are found deriving from the ipsilateral nucleus ventromedialis thalami, nucleus centralis posterior, dorsal periventricular hypothalamus, and torus semicircularis. axons arising in the pgl can ... | 1986 | 2429725 |
| studies on immunological properties of enolase from carp muscles (cyprinus carpio) after chemical modification of some amino-acid residues. | after modification of histidine residues, enolase from carp muscles lost the ability to precipitate with antibodies against native enzyme. this ability remained after nitration of tyrosine residues, but the immunoelectrophoretic pattern--localisation and height of arcs--differed from that of native enolase. minor changes of this pattern were found in enolase with modified tryptophan residues. | 1986 | 2430546 |
| preparation and some properties of carp heavy meromyosin. | trypsin fragments carp myosin heavy chain into components of lower molecular weight. these changes are concomitant with the loss of ca2+-atpase and a weakening in actin-myosin interaction. as heavy meromyosin (hmm) prepared from myofibrils is more homogeneous, the above changes are due to overfragmentation of heavy chain mass. amino-acid composition of carp and rabbit hmm's are similar, but differences exist in response to dtnb and incorporation. | 1986 | 2434053 |
| urea tolerance of myofibrillar proteins of two elasmobranchs: squalus acanthias and raja tengu. | some biochemical properties of actomyosin and myosin from elasmobranchs, squalus acanthias and raja tengu are compared with those of a freshwater (cyprinus carpio) and a marine teleost (seriola quinquiradiata). whereas ca2+-atpase of teleost actomyosins are more stable in the absence of urea, the reverse is true for elasmobranchs up to 1.0 m urea. in contrast to that of teleosts, the mg2+-atpase of s. acanthias actomyosin shows an activation in the presence of urea, where as that of r. tengu per ... | 1986 | 2434054 |
| modulatory effects of ambient temperature and antigen dose on the induction of specific tolerance to different protein antigens in carp (cyprinus carpio). | 1987 | 2438707 | |
| immune response of carps (cyprinus carpio l.) against dextran. | in carps no antibodies could be evoked by immunization with soluble high molecular weight dextran using doses of 1-5000 mg dextran per kg body weight. on the other hand, an immune response against dextran could be elicited by immunization with a vaccine of leuconostoc mesenteroides b512 as well as a dextran-bsa-conjugate. these carp anti-dextran antibodies showed precipitating and agglutinating activities with specificity against alpha linked dextran determined by the almost complete inhibition ... | 1987 | 2439389 |
| mechanism of the interaction between myelin basic protein and the myelin membrane; the role of arginine methylation. | the addition of solutions of bovine myelin basic protein to suspensions of unilamellar vesicles prepared from whole myelin suspensions results in the rapid equilibrium association of the vesicles into dimers, followed by time-dependent aggregation reactions. other cationic proteins also induce the dimerization of the vesicles and equilibrium constants for dimer formation are obtained for bovine myelin basic protein, lysozyme, polyhistidine and myelin basic protein from carp, which differs from t ... | 1987 | 2440426 |
| effect of gamma-hexachlorocyclohexane (lindane) on carp (cyprinus carpio). i. effect of chronic intoxication on humoral immunity in relation to tissue pollutant levels. | lindane, the gamma-isomer of hexachlorocyclohexane, one of the most widely used insecticides, was incorporated into carp food for 109 days. the effects of 10, 100, or 1000 ppm of lindane in food on hematocrits and on antibody production against yersinia ruckeri bacterin in sera and mucus were followed for 79 days. no effect of lindane on these parameters and on spleen weight was observed. lymphoid organs, spleen and kidney, were as highly contaminated at the beginning of the inoculation (day 30) ... | 1987 | 2440660 |
| effect of gamma-hexachlorocyclohexane (lindane) on carp (cyprinus carpio). ii. effects of chronic intoxication on blood, liver enzymes, and muscle plasmic membrane. | a 30-day ingestion of gamma-hexachlorocyclohexane (lindane) by carp (cyprinus carpio) induced hypoglycemia without activation of two hepatic gluconeogenesis enzymes (fructose diphosphatase, ec 4.1.2.13, and glucose-6-phosphatase, ec 3.1.3.9) and hyponatremia and variations in muscle plasmic membrane-bound enzymes (especially cholinesterases, ec 3.1.1.7). after 109 days carps exhibited a decrease in natremia but no significant hypoglycemia. there was an activation of gluconeogenesis enzymes. impo ... | 1987 | 2440661 |
| dense osmiophilic material in the surface of the olfactory bulb in the teleost cyprinus carpio l. | a dense osmiophilic structure was found associated to the basal lamina of the outer limiting glial membrane of the carp olfactory bulb. in certain sections this material appears as "beads" composed of small strand-like structures, and in others it seems to form a palisade-like structure. the possible origin and function of this structure is discussed. | 1987 | 2442244 |
| oxygen bonding in human hemoglobin and its isolated subunits: a xanes study. | the x-ray absorption near edge structure (xanes) spectra of the human adult and foetal hemoglobin, of the isolated alpha and beta chains, in the oxygenated forms, and of the oxymyoglobin and carp oxyhemoglobin have been measured at the wiggler beam line of the frascati synchrotron radiation facility. the bonding angle of oxygen molecule at the iron site in these hemoproteins in solution, has been measured using the multiple scattering theory for data analysis. | 1987 | 2443133 |
| sepharose gels as antigenic matrices for specific binding of anti-galactosyl antibodies. | from 100 ml of pooled normal serum taken from different species (man, cow, rabbit, chicken, duck, carp) 0.5 to 4.0 mg of high and low molecular weight anti-galactosyl antibodies could be isolated by affinity-chromatography on non-ligand-attached sepharose 4b. the antibodies bound to the agarose matrix were eluted with 0.5 m d-galactose containing buffers. using species-specific anti-immunoglobulin sera in immunoelectrophoresis, the immunoglobulin character of the protein fractions prepared was a ... | 1987 | 2444220 |
| calcium action potential and its use for measurement of reversal potentials of horizontal cell responses in carp retina. | 1. in the carp retina perfused with a solution containing high-ca2+, ba2+ and some k+-channel blockers, the horizontal cell produced a regenerative ca2+ action potential when the cell was depolarized by bath application of l-glutamate (glu) or l-aspartate (asp). the action potential was triggered also by a transretinal electrical stimulation which evoked an e.p.s.p. in the horizontal cell. in this solution, some cells produced the action potential spontaneously. 2. the action potential had an ov ... | 1987 | 2445962 |
| light microscopic characterization of glycoconjugates in secretory cells of the carp (cyprinus carpio) gill epithelium. | secretory products of granular and mucous cells in the gill epithelium of the carp, cyprinus carpio, were distinguished by their cytochemical reactions with peroxidase-labelled lectins and with the galactose oxidase (go)-schiff reagents. secretory products of granular cells reacted with lectins from triticum vulgaris (wga), arachis hypogaea (pna), dolichos biflorus (dba), glycine max (sab), and lotus tetragonolobus (lta). they also reacted with go-schiff reagents. after sialic acid cleavage with ... | 1987 | 2449406 |
| contractile response to substance p in isolated smooth muscle strips from the intestinal bulb of the carp (cyprinus carpio). | 1. the effect of substance p on the mechanical activity of carp intestinal bulb smooth muscle was investigated in vitro. 2. bath-applied substance p (1 nm-1 microm) caused concentration-dependent contraction of the smooth muscle. the ec50 value was 20 +/- 3 nm (n = 13). 3. pretreatment with tetrodotoxin (780 nm) or atropine (500 nm) partially decreased the contractile response to substance p, while methysergide (3 microm) did not decrease the response. 4. the contractile response to substance p ... | 1988 | 2455618 |
| antigenic determinants on human choriogonadotropin alpha-subunit. ii. immunochemical mapping by a monoclonal antipeptide antibody. | in order to study antigenic site(s) present in the carboxyl-terminal part of the alpha-subunit of human choriogonadotropin (hcg-alpha), we attempted to produce site-specific antibodies directed against a 34-residue synthetic peptide analogous to region 59-92 of hcg-alpha. from a fusion experiment performed with a mouse injected with hcg-alpha-(59-92)-peptide conjugated to tetanus toxoid as immunogen, we selected a monoclonal antipeptide antibody (designated fa36) which has high binding activity ... | 1988 | 2455715 |
| effect of pesticides (atrazine and lindane) on the replication of spring viremia of carp virus in vitro. | atrazine and lindane are widely employed as pesticides in agriculture, which by wash off, could contaminate the aquatic environment. in this study, they were tested in vitro on the replication of the virus of spring viremia of carp (svc) in epithelioma papulosum cyprini cell line. they were dissolved first in dmso and then in medium at their solubility limit in water (28 and 10 mg/l). the results of the kinetic titers after 72 h showed no statistically significant difference between control and ... | 1988 | 2461139 |
| the immune response of carp against dextran. an analysis of the idiotype heterogeneity. | the expression of a "species-specific" idiotype with specificity for alpha(1-6) dextran in carp is described. all tested carp anti-dextran antisera react with a rabbit anti-id (carp anti-dex) serum. the rabbit anti-id serum can be partially inhibited by the specific ligand, dextran t70 or isomaltodecaose but not or only weak with other, unrelated carbohydrates. these data confirm our statement, that a limited germline v-gene repertoire may code for antibodies in fish. | 1988 | 2463185 |
| presence of a substance p-like peptide in an acid extract of the intestinal bulb of the carp (cyprinus carpio). | 1. the effect of an acid extract of the carp intestinal bulb (eci) on guinea-pig ileum longitudinal smooth muscle (gplm) and carp intestinal bulb longitudinal smooth muscle (ciblm) was examined. 2. eci caused a concentration-dependent contraction of gplm and ciblm. this eci-induced response was reduced by atropine to 30-40% of the control, indicating that part of the contracting activity of eci is attributable to acetylcholine. the atropine-resistant contracting activity of eci was not mediated ... | 1988 | 2464388 |
| anti-dextran antibodies of carp detected by the enzyme-linked immunosorbent assay (elisa). | carp produce anti-alpha (1-6) dextran antibodies after immunization with a vaccine of leuconostoc mesenteroides b512. these antibodies can be determined by passive haemagglutination, quantitative precipitation or by elisa. in our hands the elisa has proved to be 100 times more sensitive than the passive haemagglutination test. we could not found any correlation between the anti-dextran activity estimated with the passive haemagglutination on the one and the elisa on the other hand. | 1988 | 2465680 |
| evidence that a substance p-like peptide mediates the non-cholinergic excitatory response of the carp intestinal bulb (cyprinus carpio). | the participation of substance p in the noncholinergic contraction induced by transmural stimulation (tms) of the carp intestinal bulb was examined. in the presence of atropine, substance p caused the contraction of carp intestinal bulb smooth muscle in a concentration dependent manner (1 nmol/l - 1 mumol/l). the ec50 value was 28 +/- 7 nmol/l (n = 6). substance p-induced desensitization (1 mumol/l for 15 min), decreased the response to substance p and the atropine-resistant contraction induced ... | 1988 | 2467211 |
| optic nerve regeneration in adult fish and apolipoprotein a-i. | fish optic nerves, unlike mammalian optic nerves, are endowed with a high capacity to regenerate. injury to fish optic nerves causes pronounced changes in the composition of pulse-labeled substances derived from the surrounding non-neuronal cells. the most prominent of these injury-induced changes is in a 28-kilodalton (kda) polypeptide whose level increases after injury, as revealed by one-dimensional gel electrophoresis and autoradiography. the present study identified as apolipoprotein a-i (a ... | 1989 | 2494299 |
| an antioxidant dependent in vitro response of rainbow trout (salmo gairdneri) somatotrophs to carp growth hormone-releasing factor (grf). | 1989 | 2515141 | |
| acetylcholinesterase in the alimentary canal of fish: light and electron microscopic detection, quantitative distribution in different segments of the gut. | the acetylcholinesterase activity was measured and histochemically localized in the alimentary tract of 2 fish species, carp (cyprinus carpio) and tench (tinca tinca). a comparison was made of the activities in the different gut segments. light and electron microscopic histochemistry revealed acetylcholinesterase-positive cell bodies along the entire length of the alimentary canal in both species, between the muscular layers. acetylcholinesterase-positive, cholinergic motor endplates were freque ... | 1989 | 2516680 |
| structural differences between the ca2+-atpase enzymes of sarcoplasmic reticulum membrane from rabbit and carp muscles. | 1. structural features were compared in sarcoplasmic reticulum ca2+-atpase enzymes from carp (cyprinus carpio l.) and rabbit muscles. 2. both membrane preparations contained the 105,000 mol. wt ca2+ pump protein in high local density. 3. the tryptic cleavage of the carp enzyme gave different peptide fragments from those observed from rabbit enzyme. 4. addition of vanadate, ca2+ or lanthanides did not cause two-dimensional ca2+-atpase crystal formation, in contrast to the rabbit enzyme, which for ... | 1989 | 2522383 |
| the adductor mandibulae muscle in teleost fish with protrusible or non protrusible jaws: a histochemical and immunohistochemical study. | a study was made of the morphology and fibre type composition of the adductor mandibulae (am) muscle in teleosts with very protrusible (carp), moderately protrusible (cod) and non-protrusible (trout and cat-fish) jaws. in contrast to the trout and cat-fish, in which the am is formed by only 2 components (mandibular and mental), in the carp and cod there is a third portion (maxillary) which is more or less developed in relation to the extent of jaw protrusion. fibre types were identified by means ... | 1989 | 2527478 |
| the temperature dependence of the time course of growth and decay of miniature end-plate currents in carp extraocular muscle following thermal acclimation. | 1. the effect of temperature (5-35 degrees c) on the decay and growth phases of miniature end-plate currents (mepcs) was investigated in extraocular muscle from freshwater carp acclimated to either high (28 degrees c) or low (8 degrees c) temperature. 2. the temperature dependence of the time constant of decay (td) was found to follow an arrhenius relationship; the relationship between logtd and reciprocal of absolute temperature (1/k) being linear in both groups. the td of mepcs recorded from c ... | 1989 | 2533239 |
| comparison of calpain i and calpain ii from carp muscle. | 1. the content of calpain ii is 3.4 times more than that of calpain i when estimated by the elution profiles from a column of deae-cellulose. 2. calpain i required 1 mm ca2+ and calpain ii required 5 mm ca2+ to show the full activities. these data demonstrated that ca2+-sensitivities of both calpains were lower than those of mammalian calpains, respectively. 3. the optimum caseinolytic activity was ph 7.2 for calpain i and ph 7.5 for calpain ii. 4. the molecular weight of calpain i was estimated ... | 1989 | 2539943 |
| inward rectifier produced by xenopus oocytes injected with mrna extracted from carp olfactory epithelium. | ionic channels encoded by mrna extracted from carp olfactory epithelium were investigated by injection into xenopus laevis oocytes. the oocytes expressed an inward rectifier k+ -channel, as detected under two-electrode voltage clamp conditions. the results were as follows. an inactivating inward current appeared on hyperpolarization and increased with increasing extracellular k+ concentrations. the 0 current potentials plotted as a function of log [k+]0 in the range between 2 to 20 mmk+ fell on ... | 1989 | 2541514 |
| spin equilibria in human methemoglobin: effects of bezafibrate and inositol hexaphosphate as measured by susceptometry and visible spectroscopy. | the effects of inositol hexaphosphate (ihp) and a second allosteric effector, bezafibrate, on the spin-state equilibria of the mixed-spin derivatives of ferric human hemoglobin a are examined. changes in spin-state equilibrium are monitored by measuring absorption spectra in the visible region (460-700 nm) as well as by direct measurements of magnetic susceptibility by means of a superconducting fluxmeter. the addition of ihp at ph 6.5 results in a measurable shift in the spin equilibria of thes ... | 1989 | 2548609 |
| spin equilibrium in human methemoglobin: effects of inositol hexaphosphate and bezafibrate as measured by resonance raman spectroscopy. | the spin equilibria of several derivatives of human methemoglobin were probed by resonance raman scattering. the intensity of lines in the raman spectrum gives a measure of the high-spin (s = 5/2) to low-spin (s = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility measurements. the addition of bezafibrate (bzf) to methemoglobin in the absence of organic phosphate, ihp, has very little effect on the spin equilibrium, whereas in the presence of ihp ... | 1989 | 2548610 |
| the sympathetic neuro-melanophore transmission in a fresh-water indian major carp, labeo rohita (ham.). | adrenaline was effective in aggregating the melanosomes both in innervated as well as denervated melanophores. isotonic kcl could induce pigment aggregation only in innervated melanophores. adrenaline- and k+-induced pigment aggregation response in these melanophores was blocked by phentolamine: propranolol failed to do so. it is suggested that the chromatic nerves in the fish, labeo rohita are adrenergic and via post-synaptic alpha-adrenoceptors, control the melanosome aggregation. | 1989 | 2550366 |
| distribution of angiotensin i converting enzyme in male reproductive systems of various vertebrates and properties of the genital enzymes. | the distribution of the angiotensin i converting enzyme (ec 3.4.15.1, ace) in male reproductive systems of various vertebrates including non-mammalian species and properties of the genital aces were studied. in such mammals as rat, dog and pig, it has been found that ace activity is only distributed in testis and epididymis (especially in the epididymal semen), but not in accessory sex glands such as prostate, seminal vesicle and coagulating gland. in the rat and dog, most of or all epididymal a ... | 1989 | 2558511 |
| decoupling of horizontal cells in carp and turtle retinae by intracellular injection of cyclic amp. | 1. horizontal cells are electrically coupled through gap junctions. this is a disadvantage in elucidating the membrane properties of the cells. in order to block gap junctions, adenosine 3',5'-cyclic monophosphate (cyclic amp) or its analogues, dibutyryl cyclic amp and 8-bromo cyclic amp, were ionophoretically injected into horizontal cells of the carp or turtle retina. 2. before injection of the chemicals the input resistance of the cell was so low as to be unmeasurable, because the applied cur ... | 2008 | 2559975 |
| elevation of sex steroids and inhibition of udp-glucuronyltransferase are out of phase during gonadal maturation in the common carp. | 1. plasma sex steroid concentrations, onset of gonadal maturation, and hepatic microsomal udp-glucuronyltransferase (udpgt) activities were followed under natural temperature and photoperiod in outdoor tanks, and under controlled laboratory temperature and photoperiod regimens in common carp (cyprinus carpio). 2. decreased activity of udpgt was out of phase with elevations in plasma testosterone and 17 beta-estradiol during gonadal maturation. 3. injection of pituitary extract induced final gona ... | 1989 | 2565184 |
| glucuronides in mussel mytilus galloprovincialis as a possible biomonitor of environmental carcinogens. | 1. the in vitro incubation of mussel digestive gland with 1 mm aminofluorene resulted in the formation of glucuronides that (a) became mutagenic with carp liver s9, and (b) liberated s9-dependent mutagenic aglucones after beta-glucuronidase treatment. 2. natural populations of mussels from unpolluted and polluted sites, as well as mussels exposed to 3 ppm of aminofluorene or to used engine oil, did not accumulate detectable amounts of premutagens, mutagens, or mutagenic glucuronides/aglucones ei ... | 1989 | 2565191 |
| some characteristics of mitochondrial monoamine oxidase activity in eggs of carp (cyprinus carpio) and rainbow trout (salmo gairdneri). | 1. monoamine oxidase (mao) activity towards tryptamine, 5-hydroxytryptamine (5-ht) and phenylethylamine (pea) has been measured in mitochondria isolated from carp and trout eggs. 2. in carp eggs all the tested substrates are metabolized and the highest affinity is found with tryptamine. in trout eggs a consistent level of mao activity is obtained using tryptamine. 3. the inhibition dose-response curves of clorgyline and deprenyl indicate that both in carp and trout eggs there is only one form of ... | 1989 | 2565193 |
| histamine directly acts on beta-adrenoceptors as well as h1-histaminergic receptors, and causes positive inotropic effects in isolated ventricular muscles of carp heart (cyprinus carpio). | 1. the mechanism for positive and negative inotropic effects of histamine was studied in electrically stimulated ventricular strips of carp heart. 2. a high concentration of histamine (1 mm) caused a transient negative, and subsequent positive inotropic effects. the positive effect was significantly reduced by pyrilamine, diphenhydramine or dl-propranolol, but was not affected by cimetidine or d-propranolol. 3. prior treatment with reserpine significantly decreased epinephrine and norepinephrine ... | 1989 | 2566436 |
| the roles of alpha- and beta-adrenoceptors in the chronotropic responses to norepinephrine in carp heart (cyprinus carpio). | 1. the chronotropic effect of norepinephrine was studied in isolated spontaneously beating atrial preparations of carp (cyprinus carpio) heart. 2. norepinephrine, 0.1 microm, caused a positive chronotropic effect, while at 1 microm it caused either a positive or a negative chronotropic effect. the positive chronotropic effect, observed in 13 preparations, was potentiated by phentolamine and almost completely blocked by propranolol. 3. the negative chronotropic effect observed in the other 5 prep ... | 1989 | 2566437 |
| d-aspartate potentiates the effects of both l-aspartate and l-glutamate on carp horizontal cells. | l-aspartate, l-glutamate and d-aspartate, alone or in combination, were applied by superfusion or by atomization to the isolated carp retina while recording from cone horizontal cells. each of these agents when applied alone depolarized horizontal cells and reduced the size of their light responses, an action which mimics the effect of the endogenous photoreceptor transmitter. application of d-aspartate in conjunction with either of the l-amino acids potentiated the effects the l-amino acids so ... | 1989 | 2573859 |
| production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain s-100 protein. | balb/c mice were immunized with s-100 protein, which was isolated from bovine brain. the first fusion resulted in the cloning of three stable hybridoma lines (s1-61-64, s1-61-65, s1-87-4) that produced monoclonal antibodies against s-100 protein. the hybridoma lines obtained from a second fusion (s2-20, s2-95) were not stable and antibody production ceased early during cloning. immunoblotting results showed that all antibodies reacted with antigenic determinants shared by both the alpha- and bet ... | 1985 | 2578587 |
| phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. | of eight monoclonal antibodies raised against human transferrin, one (h.tf-14) cross reacted with pig and rabbit transferrins and one (h.tf-1) showed cross-reactivity with horse and dog transferrins. while rabbit and pig transferrins exhibited the same patterns of binding to molt-3 cell receptors as human and horse transferrins, binding of mouse and dog transferrins was weaker and bovine and carp transferrins gave entirely negative results. the results of these competitive binding experiments we ... | 1985 | 2578807 |