Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| development and evaluation of pcr methods based on cytochrome c oxidase subunit one (cox1) and nadh dehydrogenase subunit one gene (nad1) to detect opisthorchis viverrini in human fecal samples. | opisthorchis viverrini is highly prevalent throughout southeast asia. chronic infection of this parasite leads to cholangiocarcinoma (cca), a fatal bile duct cancer. the early and accurate detection of this parasite is very important; therefore, new pcr methods targeting cytochrome c oxidase subunit one and nadh dehydrogenase subunit one gene to detect o. viverrini in fecal specimens have been developed. ninety o. viverrini-positive human fecal samples were used in this study. the pcr sensitivit ... | 2015 | 26239798 |
| combination of praziquantel and aspirin minimizes liver pathology of hamster opisthorchis viverrini infection associated cholangiocarcinoma. | opisthorchiasis is one of the major risk factors for cholangiocarcinoma (cca) in northeastern thailand. an effective drug for killing this parasite is praziquantel. recently, several reports have shown that with frequent use, praziquantel may itself be a cca risk and can cause liver cell damage from an immunopathological response after parasite death. aspirin has many properties including anti-inflammation and anti-cancer. therefore, we use of aspirin (as) and praziquantel (pz) to improve hepato ... | 2016 | 26277607 |
| intestinal helminths recovered from humans in xieng khouang province, lao pdr with a particular note on haplorchis pumilio infection. | a survey of intestinal helminths was undertaken in riparian people in xieng khouang province, lao pdr. fecal specimens were collected from 643 people (289 males and 354 females) residing in 4 districts (nonghet, kham, phoukout, and pek) and were examined by the kato-katz technique. the overall helminth egg positive rate was 41.2%, and hookworms revealed the highest prevalence (32.7%) followed by trichuris trichiura (7.3%) and ascaris lumbricoides (5.6%). the positive rate for small trematode egg ... | 2015 | 26323842 |
| infection status of zoonotic trematode metacercariae in fishes from vientiane municipality and champasak province in lao pdr. | the infection status of fishborne zoonotic trematode (fzt) metacercariae was investigated in fishes from 2 localities of lao pdr. total 157 freshwater fishes (17 species) were collected in local markets of vientiane municipality and champasak province in december 2010 and july 2011, and each fish was examined by the artificial digestion method. total 6 species of fzt metacercariae, i.e., opisthorchis viverrini, haplorchis taichui, haplorchis yokogawai, haplorchis pumilio, centrocestus formosanus ... | 2015 | 26323843 |
| pathological characteristics of intraductal polypoid neoplasms of bile ducts in thailand. | intraductal papillary or tubular neoplasms of the bile duct have recently been proposed as one of the pre-invasive lesions of cholangiocarcinoma. herein, a total of 50 cases of intraluminal polypoid neoplasms of the bile ducts experienced in khon kaen university hospital in thailand were pathologically examined. these cases presumably had a history of infection of opisthorchis viverrini. these neoplasms were histologically composed of high-grade intraepithelial neoplasm showing a tubular and/pap ... | 2015 | 26339398 |
| immunodiagnosis of opisthorchiasis using parasite cathepsin f. | opisthorchis viverrini, a food-borne trematode parasite endemic in the lower mekong countries, is conventionally diagnosed by stool examination. however, parasitological stool-based diagnosis can be unreliable in light infections. the goal of this study was to develop the immunodiagnosis of opisthorchiasis using cathepsin f cysteine protease of o. viverrini in both indirect and sandwich elisa assays. a recombinant o. viverrini cathepsin f (rov-cf) of 40 kda was expressed in e. coli strain bl21 ( ... | 2015 | 26344868 |
| whole transcriptome profiling of adult and infective stages of the trematode opisthorchis felineus. | opisthorchis felineus, the trematode belonging to the family opisthorchiidae, is a causative agent of the infection called opisthorchiasis or liver fluke infection. being a close relative of opisthorchis viverrini and clonorchis sinensis (oriental liver flukes) it is encountered in northern eurasia, especially in russia, kazakhstan, belarus, ukraine, and baltic countries. whole genome data for oriental liver flukes revealed their adaptations for life in the bile duct but our knowledge of o. feli ... | 2016 | 26363139 |
| anticancer activity of atractylodes lancea (thunb.) dc in a hamster model and application of pet-ct for early detection and monitoring progression of cholangiocarcinoma. | opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) is an important cancer in the great mekong region, particularly in thailand. limitations of treatment options and the lack of an effective diagnostic tool for early detection of cca are major concerns for the control of this type of cancer. the aim of the study was to investigate anti-cca activity of the ethanolic extract of atractylodes lancea (thunb.) dc., and the applicability of positron emission tomography-computed tomography (pet ... | 2015 | 26434829 |
| upregulation of 14-3-3 eta in chronic liver fluke infection is a potential diagnostic marker of cholangiocarcinoma. | to discover protein markers in chronic/advanced opisthorchiasis for the early detection of opisthorchis viverrini (ov)-associated cholangiocarcinoma (cca). | 2016 | 26435198 |
| urinary microrna-192 and microrna-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group. | opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (pdf), which possibly associates to cholangiocarcinoma (cca). patients with cca have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. hence, detecting early stage cca is essential. secretory mirnas have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. we aimed to determine levels of mir-192 a ... | 2015 | 26456596 |
| the mirnaome of opisthorchis viverrini induced intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is an aggressive cancer, arising in the biliary ducts that extend into the liver. the highest incidence of icc occurs in southeast asia, particularly in the mekong river basin countries of thailand, laos, cambodia, and vietnam, where it is strongly associated with chronic infection by the food-borne liver fluke opisthorchis viverrini (ov), one of only three eukaryote pathogens considered group one carcinogens. intrahepatic cholangiocarcinoma is usually diagn ... | 2014 | 26484108 |
| advances in the diagnosis of human opisthorchiasis: development of opisthorchis viverrini antigen detection in urine. | many strategies to control opisthorchiasis have been employed in thailand, but not in the other neighbouring countries. specific control methods include mass drug administration (mda) and health education to reduce raw fish consumption. these control efforts have greatly shifted the epidemiology of opisthorchis viverrini (ov) infection over the last decade from presenting as densely concentrated "heavy" infections in single villages to widespread "light" ov infections distributed over wide geogr ... | 2015 | 26485024 |
| carcinogenic parasite secretes growth factor that accelerates wound healing and potentially promotes neoplasia. | infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). injury from feeding activities of this parasite within the human biliary tree causes extensive lesions, wounds that undergo protracted cycles of healing, and re-injury over years of chronic infection. we show that o. viverrini secreted proteins accelerated wound resolution in human cholangiocytes, an outcome that was compromised following silencing of expression of the fluke-de ... | 2015 | 26485648 |
| association between praziquantel treatment and cholangiocarcinoma: a hospital-based matched case-control study. | infection by the liver fluke, opisthorchis viverrini, remains an important public health problem in thailand and has resulted in the highest prevalence of infection and incidence of subsequent cholangiocarcinoma (cca) in the world. praziquantel (pzq) is the antihelminthic drug of choice for treatment. previous studies in hamsters showed that repeated infection and pzq treatment could increase the risk of cca. however, the few available epidemiology studies in humans have shown unclear evidence o ... | 2015 | 26496745 |
| human trichostrongyliasis: a hospital case series. | trichostrongylus is a common nematode found to infect livestock throughout the tropics and can cause accidental zoonosis in humans. in the lao pdr and thailand, cases of human trichostrongyliasis have been reported sporadically but clinical data are limited. we retrospectively reviewed 41 cases of trichostrongyliasis who presented to srinagarind hospital, thailand from 2005 to 2012. the diagnosis of trichostrongyliasis was made by finding their eggs in the stool of patients. of the 41 cases revi ... | 2015 | 26513921 |
| review and current status of opisthorchis viverrini infection at the community level in thailand. | opisthorchis viverrini is remains a public health problem in thailand, particularly in the northeast and north regions which have the highest incidences of chonalgiocarcinoma (cca). o. viverrini causes the disease opithorchiasis, and its has been classified as a group 1 biological carcinogen. humans, dogs, and cats become infected with o. viverrini by ingesting raw or undercooked fish containing infective metacercariae. the first human cases of o. viverrini infection were reported in thailand 10 ... | 2015 | 26514452 |
| community participation in cholangiocarcinoma prevention in ubon ratchathani, thailand: relations with age and health behavior. | a high prevalence of opisthorchis viverrini infection is usually found in wetland geographical areas of thailand where people have traditional behavior of eating uncooked freshwater fish dishes which results in cholangiocarcinoma (cca) development. there were several approaches for reducing opisthorchiasis-linked cca, but the prevalence remains high. to develop community participation as a suitable model for cca prevention is, firstly, to know what factors are related. we therefore aimed to inve ... | 2015 | 26514539 |
| development and validation of an enantioselective lc-ms/ms method for the analysis of the anthelmintic drug praziquantel and its main metabolite in human plasma, blood and dried blood spots. | praziquantel (pzq) is the treatment of choice against various trematode and cestode infections. to study the pharmacokinetics of pzq in patients infected with the liver fluke opisthorchis viverrini, we developed and validated an enantioselective liquid chromatography coupled to tandem mass spectrometry method for the analysis of r - and s -pzq and its r -trans-4-oh-pzq metabolite in human plasma, blood and dried blood spots (dbs). the analytes were detected in the positive mode using selected re ... | 2016 | 26517852 |
| trematode infection of freshwater snail, family bithyniidae in thailand. | opisthorchis viverrini is restricted to and requires for its aquatic life cycle only bithynia snail as first intermediate host but many species of cyprinid fish as second intermediate hosts. a survey in thailand of trematode infection in freshwater snails of the family bithyniidae carried out during october 2008 - july 2009 found a total of 5,492 snails, classified into ten species distributed in various geographic areas. bithyniafuniculata and gabbia pygmaea were localized to the north, b. s. g ... | 2015 | 26521513 |
| bithynia siamensis goniomphalos, the first intermediate host of opisthorchis viverrini in thailand. | opisthorchiasis caused by opisthorchis viverrini (o. viverrini) remains as medically important problem in thailand especially in the north-eastern part. infection with this parasite can lead to cholangiocarcinoma improvement. the highest prevalence of o. viverrini infection has been found in the northeast thailand and is associated with the high incidence rate of cholangiocarcinoma. to complete the life cycle of o. viverrini, the freshwater snails namely bithynia funiculata, bithynia siamensis s ... | 2015 | 26522291 |
| diagnosis of opisthorchis viverrini infection with handheld microscopy in lao people's democratic republic. | opisthorchiasis is a neglected tropical disease, yet it is of considerable public health importance in southeast asia given the predilection for chronically infected persons to develop cholangiocarcinoma. we evaluated a handheld microscope for the diagnosis of opisthorchis viverrini in a community-based setting in lao people's democratic republic in comparison with conventional light microscopy. in stool samples collected from 104 individuals, handheld microscopy revealed a sensitivity of 70.6% ... | 2016 | 26526923 |
| nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in opisthorchis viverrini-infected hamsters. | this study investigated the effects of nanoencapsulated curcumin (nec) and praziquantel (pzq) treatment on the resolution of periductal fibrosis (pdf) and bile canalicular (bc) abnormalities in opisthorchis viverrini infected hamsters. chronic o. viverrini infection (ov) was initially treated with either pzq (op) and subsequently treated with nec (op+nec), curcumin (op+cur) or unloaded carriers (op+carrier) daily for one month. op+nec treatment reduced the pdf by suppression of fibrotic markers ... | 2016 | 26542278 |
| effects of fermentation time and low temperature during the production process of thai pickled fish (pla-som) on the viability and infectivity of opisthorchis viverrini metacercariae. | contamination of a popular fermented fish dish, pla-som, by opisthorchis viverrini metacercariae (ovmc) is a possible cause of carcinogenic liver fluke infection in thailand. affected individuals are at risk of bile duct cancer, which is a major health problem for people in the greater mekong subregion. in order to investigate concerns about food safety, we studied the effects of fermentation time and low temperature on the viability and infectivity of ovmc during the pla-som production process. ... | 2016 | 26587967 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsist ... | 2015 | 26618199 |
| functional analysis of the unique cytochrome p450 of the liver fluke opisthorchis felineus. | the basic metabolic cytochrome p450 (cyp) system is essential for biotransformation of sterols and xenobiotics including drugs, for synthesis and degradation of signaling molecules in all living organisms. most eukaryotes including free-living flatworms have numerous paralogues of the cyp gene encoding heme monooxygenases with specific substrate range. notably, by contrast, the parasitic flatworms have only one cyp gene. the role of this enzyme in the physiology and biochemistry of helminths is ... | 2015 | 26625139 |
| the carcinogenic liver fluke opisthorchis viverrini among rural community people in northeast thailand: a cross- sectional descriptive study using multistage sampling technique. | opisthorchis viverrini infection is a serious public health problem in southeast asia especially in the northeast and north of thailand. therefore, a cross-sectional survey using multistage sampling was conducted from the rural communities of surin province, thailand, during september 2013 to july 2014. o. viverrini infection was determined using kato's thick smear technique. socio-demographic, information resources, and history data were collected using predesigned semi-structured questionnaire ... | 2015 | 26625801 |
| increase in l-type amino acid transporter 1 expression during cholangiocarcinogenesis caused by liver fluke infection and its prognostic significance. | l-type amino acid transporter 1 (lat1) is highly expressed in various human cancers, including cholangiocarcinoma (cca), the most common cancer in northeast thailand. chronic inflammation and oxidative stress induced by liver fluke, opisthorchis viverrini, infection has been recognized as the major cause of cca in this area. we show here that an increased expression of lat1 and its co-functional protein cd98 are found during carcinogenesis induced by ov in hamster cca tissues. we also demonstrat ... | 2015 | 26657242 |
| clonorchis sinensis and opisthorchis spp. in vietnam: current status and prospects. | clonorchis sinensis and opisthorchis viverrini are clinically important small liver flukes because of their known association with development of cholangiocarcinoma. in vietnam, high prevalence of c. sinensis infection in humans was previously reported in northern provinces, and o. viverrini infection has been detected in several central provinces. however, diagnosis of c. sinensis and o. viverrini infections in the past was merely based on faecal egg examination. this method alone can lead to m ... | 2016 | 26740358 |
| comparison of infectivity, metacercarial burden and host mortality induced by opisthorchis viverrini sensu lato cercariae from lao pdr compared with thailand in cyprinid fish, barbonymus gonionotus. | previous studies indicate that opisthorchis viverrini sensu lato (s.l.) is a species complex with strong associations to geographical regions, i.e., specific wetlands. the present study was conducted to compare the infectivity, establishment and metacercarial burden and survival of the fish host following exposure to o. viverrini s.l. cercariae isolates from two different river wetlands. | 2016 | 26740362 |
| genomics of worms, with an emphasis on opisthorchis viverrini - opportunities for fundamental discovery and biomedical outcomes. | neglected tropical diseases cause substantial morbidity and mortality in animals and people globally. opisthorchiasis is one such disease, caused by the carcinogenic, asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is known to be associated with malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia, including thailand, lao people's democratic republic (pdr) and cambodia. no vaccine is available, and only one drug (praziquantel) is routinely emplo ... | 2016 | 26792076 |
| phylogenetic characterization of clonorchis sinensis proteins homologous to the sigma-class glutathione transferase and their differential expression profiles. | glutathione transferase (gst) is one of the major antioxidant proteins with diverse supplemental activities including peroxidase, isomerase, and thiol transferase. gsts are classified into multiple classes on the basis of their primary structures and substrate/inhibitor specificity. however, the evolutionary routes and physiological environments specific to each of the closely related bioactive enzymes remain elusive. the sigma-like gsts exhibit amino acid conservation patterns similar to the pr ... | 2016 | 26792248 |
| xanthohumol inhibits stat3 activation pathway leading to growth suppression and apoptosis induction in human cholangiocarcinoma cells. | stat3 plays a significant role in the development of cholangiocarcinoma (cca) associated with the liver fluke (opisthorchis viverrini; ov). xanthohumol (xn), a prenylated flavonoid extracted from hops, has known anticancer activity and could potentially target stat3. the present study determined the effect of xn on stat3, as well as ascertained its usefulness against cca. the cca cell proliferation at 20 µm and 50 µm of xn was shown to inhibited, while 20 µm partially inhibited il-6-induced stat ... | 2016 | 26794001 |
| the complete mitochondrial genome of metorchis orientalis (trematoda: opisthorchiidae): comparison with other closely related species and phylogenetic implications. | metorchis orientalis (trematoda: opisthorchiidae) is an important trematode infecting many animals and humans, causing metorchiasis. in the present study, the complete mitochondrial (mt) genome of m. orientalis was sequenced. the complete mt genome of m. orientalis is 13,834 bp circular dna molecule and contains 12 protein-coding genes, 22 transfer rna genes, and two ribosomal rna genes. the gene content and arrangement of m. orientalis is the same as those of opisthorchiidae trematodes (opistho ... | 2016 | 26805437 |
| hemozoin is a product of heme detoxification in the gut of the most medically important species of the family opisthorchiidae. | many species of trematodes such as schistosoma spp., fasciola hepatica and echinostoma trivolvis are blood-feeding parasites. nevertheless, there is no consensus on the feeding habits of the family opisthorchiidae (opisthorchis felineus, opisthorchis viverrini and clonorchis sinensis). previously, histological studies of o. felineus and c. sinensis revealed some dark stained material in their gut lumen. in this study we conducted a comprehensive analysis of the gut contents of three members of t ... | 2016 | 26812025 |
| andrographolide ameliorates beta-naphthoflavone-induced cyp1a enzyme activity and lipid peroxidation in hamsters with acute opisthorchiasis. | opisthorchis viverrini (ov) infection generates oxidative stress/free radicals and is considered as a primary cause ofcholangiocarcinoma since it primarily triggers sclerosing cholangitis. | 2016 | 26817235 |
| untangling the complexity of liver fluke infection and cholangiocarcinoma in ne thailand through transdisciplinary learning. | this study demonstrates how a transdisciplinary learning approach provided new insights for explaining persistent opisthorchis viverrini infection in northern thailand, as well as elucidating problems of focusing solely on the parasite as a means of addressing high prevalence of cholangiocarcinoma. researchers from diverse backgrounds collaborated to design an investigative homestay program for 72 singaporean and thai university students in five northeast thai villages. the students explored how ... | 2016 | 26822781 |
| people participation towards opisthorchis viverrini prevention and control in chaiyaphum province, northeastern thailand. | this cross-sectional descriptive and qualitative study was aimed to study the people participation and their approaches toward the human carcinogenic liver fluke, opisthorchis viverrini, prevention and control in ban chaun sub-district administrative organization (bcsao) and bamnet narong sub-district municipality (bnsm), bamnet narong district, chaiyaphum province, thailand between june 2013 and february 2014. participants were purposive selected, included chiefs of sub-district administrative ... | 2016 | 26838206 |
| re-examination of opisthorchis viverrini in nakhon ratchasima province, northeastern thailand, indicates continued needs for health intervention. | opisthorchis viverrini infection is associated with cholangiocarcinoma particularly in the cases of chronic or re-infection. this presents a serious health problem in northeastern and northern thailand. a community base approach is required for surveillance. therefore, in a pilot project, re-examination of o. viverrini infection was conducted in the 3 districts of nakhon ratchasima province, thailand, during june and october 2015. a total of 355 participants from a 194,152 population, was select ... | 2016 | 26838215 |
| serum antibody ig g and ig m titers for opisthorchis felineus correlate with eggs in faeces--a comprehensive study in chuvash republic, russia. | the cholangiocarcinoma is a. the risk of development of cholangiocarcinoma, generally a rare type of a liver tumor, increases during infection of opisthorchiasis. for this reason the timely detection of opisthorchiasis is important for cholangiocarcinoma prevention. there are many studies which concern the detection of pathogenesis of opisthorchis viverrini infection but a little known about opisthorchis felineus. in this study we investigate a correlation of the eggs which are found in a faeces ... | 2016 | 26838224 |
| helicobacter species are possible risk factors of cholangiocarcinoma. | several infectious agents are considered to be causes of cancer in human, mainly hepatitis b and c viruses, high-risk human pailloma viruses, helicobacter pylori, clonorchis sinensis, and opisthorchis viverrini. here we described the evident research and the association between helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (cca). global epidemiological studies have suggested that helicobacter spp. are possible risk factors for biliary tract diseases. molecular studie ... | 2016 | 26838240 |
| detection of the carcinogenic liver fluke opisthorchis viverrini using a mini parasep sf faecal parasite concentrator. | the mini parasep sf fecal parasite concentrator (mpsfc) is a new modification of the closed concentration system, which can easily be adopted in any routine clinical pathology laboratory. here we describe our experience with the system in diagnosing opisthorchis viverrini. a total of 199 fecal samples was submitted for routine examination in the clinical pathology laboratory of suranaree university of technology hospital, nakhon ratchasima province, thailand, during august to october 2015. out o ... | 2016 | 26838241 |
| carcinogenesis associated with parasites other than schistosoma, opisthorchis and clonorchis: a systematic review. | only three helminths (schistosoma haematobium, opisthorchis viverrini and clonorchis sinensis) are directly associated with carcinogenesis in humans whereas the role of other parasites in cancer remains unclear. this study aimed to perform a systematic review to identify recent insights in the role of other parasite infections in carcinogenesis. we conducted systematic searches of medline and embase on july 2015. our primary outcome was the association between parasitic infections and carcinogen ... | 2016 | 26840624 |
| malacological investigation of the fully operational nam theun 2 hydroelectric dam project in khammouane province, central lao pdr. | we conducted a malacological investigation in four districts of the nam theun 2 (nt2) hydroelectric dam project area, khammouane province, central lao pdr (nakai, gnommalath, mahaxai and xe bang fai), after the first and second years of full operation in march 2010 and november 2011 to determine health risks for humans. a total 10,863 snail specimens (10 families/23 species) from 57 sampling stations and 12,902 snail specimens (eight families/21 species) from 66 sampling stations were collected ... | 2015 | 26863858 |
| opisthorchis viverrini infections and associated risk factors in a lowland area of binh dinh province, central vietnam. | opisthorchiasis caused by opisthorchis viverrini is a major public health problem in the mekong basin in south east asia. it is associated with cholangiocarcinoma, a fatal cancer of the bile duct, which is very common in some areas of thailand and lao pdr. although there is evidence of opisthorchiasis in the central and southern provinces of vietnam, data are scarce and vietnam is often not considered an opisthorchiasis endemic area in the international literature. we conducted a cross-sectional ... | 2016 | 26872984 |
| proteomic characterization of the internalization of opisthorchis viverrini excretory/secretory products in human cells. | the association between liver fluke infection caused by opisthorchis viverrini and cholangiocarcinoma (cca - hepatic cancer of the bile duct epithelium) has been well established. multiple mechanisms play a role in the development of cca, but the excretory/secretory products released by o. viverrini (oves) represent the major interface between the parasite and its host, and their uptake by biliary epithelial cells has been suggested to be responsible for proliferation of cholangiocytes, the cell ... | 2016 | 26873540 |
| reinfection by opisthorchis viverrini after treatment with praziquantel. | the prevalence of infection by the liver fluke, opisthorchis viverrini (o. viverrini), has remained high in northeast thailand where it is a major risk factor for the eventual development of cholangiocarcinoma (cca). the infection is acquired by the consumption of dishes containing unsafely prepared freshwater fish, a dietary tradition which has proved resistant to change. since many people are aware that dosing with praziquantel (pzq) is a successful treatment for an episode of the infection, t ... | 2016 | 26925692 |
| pipeline for the identification and classification of ion channels in parasitic flatworms. | ion channels are well characterised in model organisms, principally because of the availability of functional genomic tools and datasets for these species. this contrasts the situation, for example, for parasites of humans and animals, whose genomic and biological uniqueness means that many genes and their products cannot be annotated. as ion channels are recognised as important drug targets in mammals, the accurate identification and classification of parasite channels could provide major prosp ... | 2016 | 26983991 |
| the complete mitochondrial genome of orthocoelium streptocoelium (digenea: paramphistomidae) for comparison with other digeneans. | orthocoelium streptocoelium is a common paramphistome species parasitizing the rumen and/or reticulum of small ruminants, leading to significant losses. this study first determined the complete mitochondrial (mt) genome of o. streptocoelium. the complete mt genome of o. streptocoelium was amplified, sequenced, assembled, analysed and then compared with those of other digeneans. the entire mt genome of o. streptocoelium is 13,800 bp in length, which is smaller than those of other digeneans except ... | 2017 | 27030161 |
| nurses and television as sources of information effecting behavioral improvement regarding liver flukes in nakhon ratchasima province, thailand. | liver fluke infection with opisthorchis viverrini and its associated cholangiocarcinoma constitute a serious problem in thailand. healthy behavior can decrease infection, therefore, the investigation of knowledge, attitude, and practice is need required in high risk areas. | 2016 | 27039731 |
| plasma peptidome as a source of biomarkers for diagnosis of cholangiocarcinoma. | cholangiocarcinoma (cca) is the bile duct cancer which constitutes one of the important public health problems in thailand with high mortality rate, especially in the opisthorchis viverrini (a parasite risk factor for cca) endemic area of the northeastern region of the country. this study aimed to identify potential biomarkers from the plasma peptidome by cca patients. peptides were isolated using 10 kda cut-off filter column and the flow-through was then used as a peptidome for lc-ms/ms analysi ... | 2016 | 27039742 |
| prevalence and factors associated with opisthorchis viverrini infection in khammouane province, lao pdr. | opisthorchis viverrini (ov) liver flukes are common parasites found in central and southern laos and constitute a major public health problem in the country. laos people continue to have the habit of extensively consuming raw or half-cooked fish which are intermediate hosts. this study aimed to study the prevalence and factors associated with ov infection in the population of thakek district, khammouane province. this cross-sectional analytic study covered 237 subjects who filled out structured ... | 2016 | 27039810 |
| wound healing and cancer progression in opisthorchis viverrini associated cholangiocarcinoma. | infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). it was shown previously that o. viverrini-secreted proteins accelerate wound resolution in human cholangiocytes. recombinant ov-grn-1 (o. viverrini-derived gene encoding granulin-like growth factor) induced angiogenesis and accelerated mouse wound healing. given the striking similarities of wound healing and cancer progression, here we discuss the major implications of this fin ... | 2016 | 27130317 |
| similarity of a 16.5kda tegumental protein of the human liver fluke opisthorchis viverrini to nematode cytoplasmic motility protein. | opisthorchis viverrini is the causative agent of human opisthorchiasis in thailand and long lasting infection with the parasite has been correlated with the development of cholangiocarcinoma. in this work we have molecularly characterized the first member of a protein family carrying two dm9 repeats in this parasite (ovdm9-1). interpro and other protein family databases describe the dm9 repeat as a protein domain of unknown function that has been first noted in drosophila melanogaster. two paral ... | 2016 | 27140280 |
| chicken igy-based coproantigen capture elisa for diagnosis of human opisthorchiasis. | diagnosis of opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. therefore sensitive and specific diagnostic test is needed. in this study, a coproantigen sandwich elisa using recombinant o. viverrini cathepsin f (rov-cf) was developed. this sandwich elisa employing chicken igy raised against rov-cf in com ... | 2016 | 27140305 |
| characterization and functional analysis of fatty acid binding protein from the carcinogenic liver fluke, opisthorchis viverrini. | in the present study, the cdna encoding fabp (ov-fabp) was isolated from the adult stage of opisthorchis viverrini and characterized. the ov-fabp protein sequence (107 amino acids) was predicted to have a molecular mass of 12.26kda and an isoelectric point (pi) of 6.82. this sequence had a high identity and similarity to cs-fabp of the related opisthorchid clonorchis sinensis. multiple sequence alignment with fabps from other parasitic flatworms and mammals showed a number of conserved amino aci ... | 2016 | 27143603 |
| decreased risk of cholangiocarcinogenesis following repeated cycles of opisthorchis viverrini infection-praziquantel treatment: magnetic resonance imaging (mri) and histopathological study in a hamster model. | it has been suggested that repeated infection of opisthorchis viverrini followed by repeated treatment with praziquantel (pzq) increases risk of development of cholangiocarcinoma (cca). evidence for the prediction has accumulated based on findings of indirect approaches involving molecular changes and epidemiological trends. by contrast, here we directly monitored the impact of repeated liver fluke infection and treatment with pzq on cholangiocarcinogenesis in a rodent model of human opisthorchi ... | 2016 | 27143606 |
| upregulation of endothelial nitric oxide synthase (enos) and its upstream regulators in opisthorchis viverrini associated cholangiocarcinoma and its clinical significance. | endothelial nitric oxide synthase (enos) is an isoform of the enzyme nitric oxide synthase (nos) which is constitutively expressed in endothelial cells and plays important roles in vasodilation. we previously reported the importance of enos activation in cholangiocarcinoma (cca) tissues and cell lines. the present study aims to investigate the relative abundance of enos and phosphorylated enos (p-enos) and their upstream regulators vegfr3, vegfc, epha3 and ephrin-a1, in the opisthorchis viverrin ... | 2016 | 27143607 |
| pharmacokinetic study of praziquantel enantiomers and its main metabolite r-trans-4-oh-pzq in plasma, blood and dried blood spots in opisthorchis viverrini-infected patients. | praziquantel (pzq) is the treatment of choice for infections with the liver fluke opisthorchis viverrini, a major health problem in southeast asia. however, pharmacokinetic (pk) studies investigating the disposition of pzq enantiomers (r- and s-pzq) and its main metabolite, r-trans-4-oh-pzq, in diseased patients are lacking. the implementation of a dried blood spot (dbs) sampling technique would ease the performance of pk studies in remote areas without clinical facilities. the aim of the presen ... | 2016 | 27152952 |
| cercarial emergence patterns for opisthorchis viverrini sensu lato infecting bithynia siamensis goniomphalos from sakon nakhon province, thailand. | opisthorchis viverrini sensu lato is a food-borne trematode which is classified as a class 1 carcinogen, with infection potentially leading to cholangiocarcinoma. snails of the genus bithynia act as the first intermediate hosts and an amplifying point in the parasite life cycle. in order to investigate seasonal effect on transmission dynamics of o. viverrini in bithynia siamensis goniomphalos, cercarial emergence and output profiles were monitored at different season. a total of 4533 snails orig ... | 2016 | 27154765 |
| cd44 variant-dependent redox status regulation in liver fluke-associated cholangiocarcinoma: a target for cholangiocarcinoma treatment. | expression of cd44, especially the variant isoforms (cd44v) of this major cancer stem cell marker, contributes to reactive oxygen species (ros) defense through stabilizing xct (a cystine-glutamate transporter) and promoting glutathione synthesis. this enhances cancer development and increases chemotherapy resistance. we investigate the role of cd44v in the regulation of the ros defense system in cholangiocarcinoma (cca). immunohistochemical staining of cd44v and p38(mapk) (a major ros target) ex ... | 2016 | 27176078 |
| characterization and differential expression analysis of toxocara canis aquaporin-1 gene. | toxocara canis is an intestinal nematode of canids with a worldwide distribution, causing an important but neglected parasitic zoonosis in humans. aquaporins (aqp) are a family of water channel proteins, which function as membrane channels to regulate water homeostasis. in this study, the coding sequence of aquaporin-1 gene of t. canis (tc-aqp-1) was cloned and characterized. the obtained tc-aqp-1 coding sequence was 933 bp in length, which predicted to encode 311 amino acids. two conserved aspa ... | 2016 | 27215210 |
| evaluation of cholangiocarcinoma risk and its related factors in wetland geographical communities of ubon ratchathani, thailand. | wetland geographical areas have a higher incidence of opisthorchis viverrini-associated cholangiocarcinoma (cca), confirmed by data from geographic information systems, than other areas. behavioral data also indicate that people in these areas traditionally eat uncooked freshwater fish dishes, a vehicle for o. viverrini infection. the best approach to reducing cca incidence is decreasing risk factors together with behavior alteration. evaluation of cca risk and its related factors are first need ... | 2016 | 27221857 |
| assessing the role of landscape connectivity on opisthorchis viverrini transmission dynamics. | opisthorchis viverrini (ov) is one of the most important human parasitic diseases in southeast asia. although the concept of connectivity is widely used to comprehend disease dispersal, knowledge of the influences of landscape connectivity on ov transmission is still rudimentary. this study aimed to investigate the role of landscape connectivity in ov transmission between the human and the first intermediate snail hosts. fieldwork was conducted in three villages respectively in kamalasai distric ... | 2016 | 27265875 |
| characterization and localization of opisthorchis viverrini fructose-1,6-bisphosphate aldolase. | opisthorchis viverrini (ov) infection is a long-time public health problem in thailand that can lead to bile duct cancer, cholangiocarcinoma (cca). characterization of the ov proteins at a molecular level will increase our knowledge of host-parasite interaction that can be applied to new drug, vaccine, or immunodiagnostic development. in this study, an important enzyme in the ov glycolytic pathway, fructose-1,6-bisphosphate aldolase (fbpa), that had been obtained from a previous study was charac ... | 2016 | 27265876 |
| overweight relation to liver fluke infection among rural participants from 4 districts of nakhon ratchasima province, thailand. | a cross-sectional survey was conducted among 730 participants from 4 rural districts of nakhon ratchasima province, thailand, with a reported high incidence of liver fluke infection. this study was aimed to examine and evaluate the nutritional status in relation to opisthorchis viverrini infection. participants were purposive selected from chum phuang, mueang yang, bua yai, and kaeng sanam nang districts. stool samples were prepared by kato katz technique and then assessed by microscopy. anthrop ... | 2016 | 27268631 |
| a combination of liver fluke infection and traditional northeastern thai foods associated with cholangiocarcinoma development. | opisthorchis viverrini infection is one of the risk factors for cholangiocarcinoma (cca) in northeast thailand, a region with one of the highest reported incidence rates of cca. the traditional practice of eating raw fish, repeated exposure to liver flukes, and consumption of nitrosamine-contaminated food are major risk factors for cca. so far, there have been no reports about which northeastern traditional dishes may be involved in cca development. the present study, thus, investigated the effe ... | 2016 | 27271702 |
| infectivity and development of opisthorchis viverrini metacercariae in immunosuppressed barbonymus gonionotus fingerlings (cyprinidae). | opisthorchiasis caused by opisthorchis viverrini infection, is a serious public health problem in lao pdr, cambodia, vietnam and thailand. cyprinid fish are required for the transmission of o. viverrini metacercariae to fish eating-mammal hosts including humans. the transmission of infective metacerariae is an important event, potentially resulting in the development of cholangiocarcinoma in cases of chronic opisthorchiasis. for a better understanding of the development of o. viverrini metacerca ... | 2016 | 27338181 |
| opisthorchis viverrini infection among people in the border areas of three provinces, northeast of thailand. | opisthorchis viverrini is still a serious problem in northeastern and northern thailand. active surveillance is required to determine updated data for further prevention and control planning. therefore, this study aimed to examine the prevalence and risk factors for o. viverrini in three provinces, northeastern thailand. a cross- sectional survey was conducted during october 2015 to march 2016 at kaeng sanam nang district of nakhon ratchasima province, waeng noi district of khon kaen province, a ... | 2016 | 27356720 |
| hypothetical granulin-like molecule from fasciola hepatica identified by bioinformatics analysis. | fasciola hepatica is considered an emergent human pathogen, causing liver fibrosis or cirrhosis, conditions that are known to be direct causes of cancer. some parasites have been categorized by who as carcinogenic agents such as opisthorchis viverrini, a relative of f. hepatica. although these two parasites are from the same class (trematoda), the role of f. hepatica in carcinogenesis is unclear. we hypothesized that f. hepatica might share some features with o. viverrini and to be responsible t ... | 2016 | 27386259 |
| chromosome morphometry in opisthorchiid species (platyhelminthes, trematoda). | few existing studies have dealt with cytogenetics in trematodes, largely due to the attendant technical difficulty of chromosome preparation. we performed a comparative analysis of chromosomes in five opistorchiid species, including opisthorchis felineus rivolta, 1884, opisthorchis viverrini poirier, 1886, clonorchis sinensis cobbold, 1875, metorchis xanthosomus creplin 1846, and metorchis bilis (braun, 1790) odening, 1962. for some of these species, no detailed morphometric description of their ... | 2016 | 27404484 |
| prevalence of trematode larvae in intermediate hosts: snails and fish in ko ae sub-district of khueang nai, ubon ratchathani province, thailand. | ko ae sub-district of khueang nai, ubon ratchathani province, thailand is located in an endemic area for opisthorchis viverrini and other fish-borne zoonotic trematodes (fzt) infection. this study shows the status in ko ae sub-district of fzt infection based on availability of intermediate hosts and necessary requirements for the transmission of fzt. a cross-sectional survey of intermediate hosts of fzt, including bithynia siamensis goniomphalos and cyprinoid fish, was conducted from april 2013 ... | 2016 | 27405122 |
| genetic differences among haplorchis taichui populations in indochina revealed by mitochondrial cox1 sequences. | haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. it is widely distributed in asia, with a particularly high prevalence in indochina. previous work revealed that the lack of gene flow between three distinct populations of vietnamese h. taichui can be attributed to their geographic isolation with no interconnected river basins. to test the hypothesis that interconnected river basins allow gene flow between otherwise isolated populations of h. taichui, as previous ... | 2016 | 27411962 |
| prevalence of opisthorchis viverrini-like fluke infection in ducks in binh dinh province, central vietnam. | following the first report of opisthorchis viverrini infection in a domestic duck in phu my district of binh dinh province, central vietnam, many other cases were observed in the province. we determined the infection rate and intensity of o. viverrini infection in ducks in 4 districts of the province. a total of 178 ducks were randomly selected from 34 farms for examination of flukes in the liver and gall bladder. an infection rate of 34.3% (range 20.7-40.4% among districts) was found; the inten ... | 2016 | 27417094 |
| clonorchis sinensis, an oriental liver fluke, as a human biological agent of cholangiocarcinoma: a brief review. | parasitic diseases remain an unarguable public health problem worldwide. liver fluke clonorchis sinensis is a high risk pathogenic parasitic helminth which is endemic predominantly in asian countries, including korea, china, taiwan, vietnam, and the far eastern parts of russia, and is still actively transmitted. according to the earlier 8th national survey on the prevalence of intestinal parasitic infections in 2012, c. sinensis was revealed as the parasite with highest prevalence of 1.86% in ge ... | 2016 | 27418285 |
| tissue microbiome profiling identifies an enrichment of specific enteric bacteria in opisthorchis viverrini associated cholangiocarcinoma. | cholangiocarcinoma (cca) is the primary cancer of the bile duct system. the role of bile duct tissue microbiomes in cca tumorigenesis is unestablished. to address this, sixty primary cca tumors and matched normals, from both liver fluke (opisthorchis viverrini) associated (ova, n=28) and non-o. viverrini associated (non-ova, n=32) cancers, were profiled using high-throughput 16s rrna sequencing. a distinct, tissue-specific microbiome dominated by the bacterial families dietziaceae, pseudomonadac ... | 2016 | 27428430 |
| population pharmacokinetic modeling of tribendimidine metabolites in opisthorchis viverrini-infected adults. | there is a pressing need for alternative treatments against the liver fluke opisthorchis viverrini oral tribendimidine is a promising candidate, but its population pharmacokinetic properties are unknown. two phase iia trials were conducted in laos in o. viverrini-infected adults receiving single oral doses of 25 to 600 mg tribendimidine administered as different formulations in each study (study 1 used 200-mg tablets, and study 2 used 50-mg tablets). venous whole blood, plasma, and capillary dri ... | 2016 | 27431233 |
| single-ascending-dose pharmacokinetic study of tribendimidine in opisthorchis viverrini-infected patients. | praziquantel is the only drug available for the treatment of opisthorchis viverrini infections. tribendimidine has emerged as a potential treatment alternative; however, its pharmacokinetic (pk) properties have not been sufficiently studied to date. via two phase iia dose-finding studies, 68 o. viverrini patients were treated with 25- to 600-mg doses of tribendimidine using 50- and 200-mg tablet formulations. plasma, blood, and dried blood spots (dbs) were sampled at selected time points. the tw ... | 2016 | 27431234 |
| morbidity assessment of opisthorchis viverrini infection in rural laos: i. parasitological, clinical, ultrasonographical and biochemical findings. | infections with the food-borne trematode opisthorchis viverinni are common in southeast asia. in lao pdr alone, two million people are supposed to be infected. opisthorchiasis may cause severe liver disease, eventually leading to cholangiocarcinoma. the objective of this study is to assess the eating habits, complaints, symptoms, signs and ultrasonographical findings in three different areas of savannakhet province. | 2016 | 27433131 |
| efficacy and safety of tribendimidine against opisthorchis viverrini: two randomised, parallel-group, single-blind, dose-ranging, phase 2 trials. | treatment of the liver fluke infection opisthorchis viverrini relies exclusively on praziquantel. tribendimidine could be an alternative treatment option. we aimed to assess the efficacy and safety of ascending single, oral doses of tribendimidine in patients with o viverrini infection. | 2016 | 27472949 |
| induction of regulatory t cells by opisthorchis viverrini. | opisthorchis viverrini causes public health problems in south-east asia. recently, tgf-β and il-10 have been reported to increase in o. viverrini-infected hamsters but the sources of these cytokines are still unknown. in this study, the cd4(+) t cells in infected hamsters were investigated. it was demonstrated that il-4(+) cd4(+) t cells were significantly increased in hamster spleens and mesenteric lymph nodes (mlns) during chronic infection. interestingly, il-10(+) cd4(+) t cells were also dis ... | 2016 | 27552546 |
| prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower northern thailand. | gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. we investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower northern thailand and utilized molecular tools for species identification of hookworms and opisthorchis viverrini. fecal samples of 197 dogs and 180 cats were collected. overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. helminth infection found in both dogs a ... | 2017 | 27570099 |
| efficacious and safe dose of praziquantel for the successful treatment of feline reservoir hosts with opisthorchiasis. | opisthorchiasis caused by opisthorchis viverrini is a major food-borne zoonosis in greater mekong sub-region. even though campaigns discouraging the consumption of raw fish have been launched to public, the disease still remains highly endemic. the unsuccessful eradication of the disease is probably because of the persistence of the parasite in animal reservoir hosts, particularly felids. praziquantel (pzq) is the drug of choice for morbidity control of opisthorchiasis in humans and animals. how ... | 2016 | 27576001 |
| modifying health behavior for liver fluke and cholangiocarcinoma prevention with the health belief model and social support theory. | the liver fluke opisthorchis viverrini is a serious health problem in thailand. infection is associated with cholangiocarcinoma (cca), endemic among human populations in northeast and north thailand where raw fish containing fluke metacercariae are frequently consumed. recently, thailand public health authorities have been organized to reduce morbidity and mortality particularly in the northeast through o. viverrini and cca screening projects. health modfication is one of activities included in ... | 2016 | 27644606 |
| health informatics model for helminthiasis in thailand. | at the beginning of the new millennium, helminth infections continue to be prevalent, particularly among impoverished populations. this study attempts to create the first health informatics model of helminthiasis in thailand. the authors investigate how a health informatics model could be used to predict the control and eradication in a national control campaign. fish-borne helminthiasis caused by opisthorchis viverrini remains a major public health problem in many parts of south-east asia, incl ... | 2016 | 27666946 |
| liver fluke infection and cholangiocarcinoma: a review. | parasites are significant groups for carcinogenesis among which liver flukes, including opisthorchis viverrini and clonorchis sinensis, are typical representatives causing cholangiocarcinoma (cca), the second most common primary hepatic malignancy with dismal prognosis. o. viverrini is prevalent in southeast asia, infecting 10 million people while c. sinensis has a wider distribution in east asia and several southeast asian countries, affecting more than 35 million people's health. these two wor ... | 2017 | 27718017 |
| cpg oligodeoxynucleotides with crude parasite antigens reduce worm recovery in opisthorchis viverrini infected hamsters. | opisthorchis viverrini, a human liver fluke, is still an endemic parasitic infection in thailand and nearly all countries in southeast asia. o. viverrini induces a chronic stage of infection in hamsters. during the first 2 weeks of infection, th1 inducing cytokine, il-12, increased but was down regulated in chronic infection. in this study it was found that unmethylated-cpg odn (oligodeoxynucleotides) 1826 increased hamster mononuclear cell proliferation and stimulated ifn-γ production in vitro. ... | 2016 | 27737764 |
| association between opisthorchis viverrini and leptospira spp. infection in endemic northeast thailand. | opisthorchiasis caused by opisthorchis viverrini is an important foodborne trematodiasis in thailand, laos and cambodia. interestingly, the opisthorchiasis endemic region overlaps with an area of leptospirosis emergence. here we report an association between opisthorchiasis and leptospirosis in thailand. of 280 sera collected from villagers living around the lawa wetland complex in khon kaen province, 199 (71%) were seropositive for leptospirosis by immunochromatography. individuals with o. vive ... | 2016 | 27746382 |
| cytokine profiles in opisthorchis viverrini stimulated peripheral blood mononuclear cells from cholangiocarcinoma patients. | the carcinogenic liver fluke opisthorchis viverrini causes chronic inflammation in the bile duct and resulting in unremitting tissue damage that lead to hepatobiliary diseases, including cholangiocarcinoma (cca). despite inflammatory cytokine expression having been studied in the animal model, so far no studies have been carried out on cytokines in human cca cases. here we report the profile of cytokine production in peripheral blood mononuclear cells (pbmcs) collected from o. viverrini-associat ... | 2017 | 27769807 |
| modeling impacts of climate change on the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand. | global climate change is now regarded as imposing a significant threat of enhancing transmission of parasitic diseases. maximum entropy species distribution modeling (maxent) was used to explore how projected climate change could affect the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand. a range of climate variables was used: the hadley global environment model 2-earth system (hadgem2-es) climate change model and also the ipcc scenarios a2a for 2050 a ... | 2017 | 27774575 |
| changing patterns of prevalence in opisthorchis viverrini sensu lato infection in children and adolescents in northeast thailand. | infection with the liver fluke opisthorchis viverrini sensu lato (s.l.), a group 1 carcinogen, is the most important risk factor for developing cholangiocarcinoma (cca) in southeast asia. cholangiocarcinoma is a fatal disease with the world's highest incidence being found in northeast thailand. liver fluke infection occurs through eating raw or partially cooked cyprinid fish containing metacercariae and, therefore, the control of o. viverrini s.l. infection should lead to a reduction in cca inci ... | 2016 | 27794488 |
| chronic opisthorchis viverrini infection changes the liver microbiome and promotes helicobacter growth. | adults of opisthorchis viverrini reside in the biliary system, inducing inflammation of bile ducts and cholangitis, leading to hepatobiliary disease (hbd) including cholangiocarcinoma. o. viverrini infection also has major implications for the bacterial community in bile ducts and liver. to investigate this in chronic o. viverrini infection (≥ 8 months p.i.), bacterial genomic dna from livers of hamsters and from worms was investigated using culture techniques, pcr for helicobacter spp. and high ... | 2016 | 27806126 |
| development of a pcr assay for diagnosing trematode (opisthorchis and haplorchis) infections in human stools. | we developed a combined conventional polymerase chain reaction (pcr) and real-time pcr (qpcr)-based assay for detecting and discriminating between opisthorchis viverrini and haplorchis taichui parasite infections. the first pcr amplifies the mitochondrial cytochrome c oxidase subunit i (coi) genes of parasites, and differential diagnosis is achieved by performing qpcr with specific primers and sybr green i. the detection limit of the assay was found to be 2.0 × 10(2) plasmid copies in a test in ... | 2017 | 27821695 |
| seasonal and spatial environmental influence on opisthorchis viverrini intermediate hosts, abundance, and distribution: insights on transmission dynamics and sustainable control. | opisthorchis viverrini (ov) is a complex-life-cycle trematode affecting 10 million people in sea (southeast asia). human infection occurs when infected cyprinid fish are consumed raw or undercooked. ov requires three hosts and presents two free-living parasitic stages. as a consequence ov transmission and infection in intermediate and human hosts are strongly mediated by environmental factors and understanding how environmental variability influences intermediate host abundance is critical. the ... | 2016 | 27880787 |
| new locality record for haplorchoides mehrai and possible interactions with opisthorchis viverrini metacercariae in cyprinid fishes in northeast thailand. | metacercariae of opisthorchis viverrini, a carcinogenic liver fluke, and haplorchoides sp., a trematode maturing in catfish, are commonly found in cyprinid fish, the second intermediate hosts of both flukes. however, the specific identity of haplorchoides sp. in thailand and a precise assessment of the effects of co-infections with o. viverrini have never been clarified. therefore, we aimed to identify the species of haplorchoides and to investigate possible interactions of the two trematode spe ... | 2017 | 27882434 |
| the lawa model: a sustainable, integrated opisthorchiasis control program using the ecohealth approach in the lawa lake region of thailand. | opisthorchiasis caused by the carcinogenic liver fluke opisthorchis viverrini is a major foodborne parasitic zoonotic disease in thailand and neighboring mekong countries. the infection is associated with several hepatobiliary diseases including cholangiocarcinoma (cca). the rates of cca in regions where the parasite is endemic are unprecedented. extensive research on various aspects of opisthorchiasis has been conducted in recent decades. however, the current status of o. viverrini infection in ... | 2016 | 27890720 |
| differential protein expression in the hemolymph of bithynia siamensis goniomphalos infected with opisthorchis viverrini. | bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke opisthorchis viverrini. this parasite is a major public health problem in different countries throughout the greater mekong sub-region (thailand, southern vietnam, lao pdr and cambodia). chronic o. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma ... | 2016 | 27893749 |
| co-infections with liver fluke and helicobacter species: a paradigm change in pathogenesis of opisthorchiasis and cholangiocarcinoma? | infection with the fish-borne liver fluke opisthorchis viverrini is classified by the international agency for research on cancer as a group 1 carcinogen: definitely carcinogenic in humans. cofactors likely contribute to bile duct cancer (cholangiocarcinoma) caused by this infection. here we review recent findings that address the role of liver fluke associated h. pylori in hepatobiliary disease and malignancy. we hypothesize that co-infection by o. viverrini and the bacillus helicobacter pylori ... | 2016 | 27919744 |
| uncovering the pathogenic landscape of helminth (opisthorchis viverrini) infections: a cross-sectional study on contributions of physical and social environment and healthcare interventions. | helminth infections have proven recalcitrant to control by chemotherapy in many parts of southeast asia and indeed farther afield. this study isolates and examines the influence of different aspects of the physical and social environment, and uneven intervention effort contributing to the pathogenic landscape of human opisthorchis viverrini infections. | 2016 | 27926938 |
| efficacy and safety of praziquantel against light infections of opisthorchis viverrini: a randomised parallel single blind dose-ranging trial. | the liver fluke opisthorchis viverrini, highly prevalent in southeast asia, is an important public health burden, including a risk factor for developing an aggressive bile duct cancer, cholangiocarcinoma, in chronically infected patients. praziquantel, administered at single 40 mg/kg in preventive chemotherapy programs and 3 x 25 mg/kg for individual treatment is the drug of choice, yet information on the nature of the dose-response relationship is lacking. | 2016 | 27927864 |
| glycoproteomics analysis of plasma proteins associated with opisthorchis viverrini infection-induced cholangiocarcinoma in hamster model. | to apply lectin affinity chromatography and glycoproteomics-based lc-ms/ms to preliminarily investigate the possible potential plasma biomarkers of opisthorchis viverrini (ov)-associated cca in ov/dimethylnitrosamine (dmn)-induced cca hamster model. | 2016 | 27955744 |