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optimized biotransformation of icariin into icariside ii by β-glucosidase from trichoderma viride using central composite design method.a crude β-glucosidase has been produced from trichoderma viride and used to explore a simple method to prepare icariside ii from icariin. the crude enzyme has been studied by zymography method and used for hydrolysis of ica. to achieve a high conversion rate of ica, various factors have been studied including ph, reaction time, temperature, initial concentration of enzyme, and initial concentration of ica through central composite design experiments. in the condition of the optimum hydrolysis pa ...201626981534
chemical composition, antimicrobial and cytotoxic activity of heracleum verticillatum pančić and h. ternatum velen. (apiaceae) essential oils.in this work, the chemical composition, antimicrobial and cytotoxic activity of heracleum verticillatum pančić and h. ternatum velen. root, leaf, and fruit essential oils were investigated. the composition was analyzed by gc and gc/ms. heracleum verticillatum and h. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (e)-caryophyl ...201626991469
nutrient assessment of olive leaf residues processed by solid-state fermentation as an innovative feedstuff additive.olive leaf residue feedstuff additives were prepared by solid-state fermentation (ssf), and its feeding effects on broiler chickens were examined.201626991541
antimicrobial activity of the pygidial gland secretion of three ground beetle species (insecta: coleoptera: carabidae).the antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, carabus ullrichii, c. coriaceus, and abax parallelepipedus, have been tested. microdilution method was applied for detection of minimal inhibitory concentrations (mics), minimal bactericidal concentrations (mbcs), and minimal fungicidal concentrations (mfcs). additionally, morpho-histology of the pygidial glands is investigated. we have tested 16 laboratory and clinical strain ...201627000261
structural and functional characterization of recombinant napin-like protein of momordica charantia expressed in methylotrophic yeast pichia pastoris.napin and napin-like proteins belong to the 2s albumin seed storage family of proteins and have been shown to display a variety of biological activities. however, due to a high degree of polymorphism, purification of a single napin or napin-like protein exhibiting biological activity is extremely difficult. in the present study, we have produced the napin-like protein of momordica charantia using the methylotrophic pichia pastoris expression system. the recombinant napin-like protein (rmcnapin) ...201627020281
biodegradation of poly(lactic acid) and some of its based systems with trichoderma viride.the purpose of this study was to assess the biodegradation of poly(lactic acid) (pla) and some plasticized pla based systems by trichoderma viride fungus, in liquid medium and controlled laboratory conditions. the studied systems were achieved using pla, hydrolyzed collagen (hc) as biological macromolecules and other additives by melt processing procedure. pla and the systems' biodegradability was examined by the weight losses of the samples (after 7 and 21 days of exposure) and ftir-atr, gpc, s ...201627064086
populations of some molds in water-damaged homes may differ if the home was constructed with gypsum drywall compared to plaster.starting in the 1940s, gypsum drywall began replacing plaster and lathe in the u.s. home construction industry. our goal was to evaluate whether some mold populations differ in water- damaged homes primarily constructed with gypsum drywall compared to plaster. the dust samples from the 2006 department of housing and urban development's (hud) american health homes survey (ahhs) were the subject of this analysis. the concentrations of the 36 environmental relative moldiness index (ermi) molds were ...201627104493
chemical composition and protein enrichment of orange peels and sugar beet pulp after fermentation by two trichoderma species.the present experiment aimed at increasing orange peel and sugar beet pulp protein content through solid-state fermentation by trichoderma reesei and trichoderma viride. in vitro digestibility and changes in the chemical composition of the fermented products were determined after seven days of fungal cultivation using gas production tests. the cultivation of t. reesei and t. viride on orange peels decreased neutral detergent soluble content (p<0.01) and increased cellulose, hemicellulose and lig ...201527175146
β-glucosidase from the hyperthermophilic archaeon thermococcus sp. is a salt-tolerant enzyme that is stabilized by its reaction product glucose.β-glucosidase (bg) is widely applied in the biofuel's industry, as part of a cellulase cocktail to catalyze the hydrolysis of the β-1,4 linkages that join two glucose molecules in a cellulose polymer. the hydrolysis step is generally recognized as the major limiting step in the development of efficient enzyme-based technologies for the conversion of lignocellulosic biomass to sugars and the production of biofuels due to the accumulation of the reaction product, glucose. relieving this glucose in ...201627198723
anthracnose of lucky bamboo dracaena sanderiana caused by the fungus colletotrichum dracaenophilum in egypt.dracaena sanderiana, of the family liliaceae, is among the ornamental plants most frequently imported into egypt. typical anthracnose symptoms were observed on the stems of imported d. sanderiana samples. the pathogen was isolated, demonstrated to be pathogenic based on koch's rule and identified as colletotrichum dracaenophilum. the optimum temperature for its growth ranges from 25 to 30 °c, maintained for 8 days. kemazed 50% wettable powder (wp) was the most effective fungicide against the pat ...201627222738
trichoderma viride laccase plays a crucial role in defense mechanism against antagonistic organisms.fungal laccases are involved in a variety of physiological functions such as delignification, morphogenesis, and parasitism. in addition to these functions, we suggest that fungal laccases are involved in defense mechanisms. when the laccase secreting trichoderma viride was grown in the presence of a range of microorganisms including bacteria and fungi, laccase secretion was enhanced in response to antagonistic organisms alone. in addition, growth of antagonistic microbes was restricted by the s ...201627242756
[biodegradation of cellulose-containing substrates by micromycetes followed by bioconversion into biogas].the ability of micromycetes trichoderma viride and aspergillus terreus to decompose the cellulose-containing substrates was studied. office paper and cardboard, as well as a paper mixture, were found to be the most hydrolyzable. the cellulolytic activity of t. viride was 2-3 times higher than that of a. terreus; the highest values of 0.80 and 0.73 u/mlwere obtained from office paper and the paper mixture, respectively. the micromycete cultivation conditions (composition of culture medium, sucros ...201627266249
physico-chemical condition optimization during biosynthesis lead to development of improved and catalytically efficient gold nano particles.biosynthesis of nanoparticles has gained great attention in making the process cost-effective and eco-friendly, but there are limited reports which describe the interdependency of physical parameters for tailoring the dimension and geometry of nanoparticles during biological synthesis. in the present study, gold nanoparticles (gnps) of various shapes and sizes were obtained by modulating different physical parameters using trichoderma viride filtrate. the particles were characterized on the basi ...201627273371
industrial wastewaters and dewatered sludge: rich nutrient source for production and formulation of biocontrol agent, trichoderma viride.axenic cultivation of biocontrol fungus trichoderma viride was conducted on a synthetic medium and different wastewaters and wastewater sludges in shake flasks to search for a suitable raw material resulting in higher biocontrol activity. soluble starch based synthetic medium, dewatered municipal sludge, cheese industry wastewater sludge, pre-treated and untreated pulp and paper industry wastewater and slaughter house wastewater (shw) were tested for t. viride conidia and protease enzyme product ...200727517824
antifungal and antibacterial activities of petroselinum crispum essential oil.parsley [petroselinum crispum (mill.) fuss] is regarded as an aromatic, culinary, and medicinal plant and is used in the cosmetic, food, and pharmaceutical industries. however, few studies with conflicting results have been conducted on the antimicrobial activity of parsley essential oil. in addition, there have been no reports of essential oil obtained from parsley aerial parts, except seeds, as an alternative natural antimicrobial agent. also, microorganism resistance is still a challenge for ...201627525894
management of blight of bell pepper (capsicum annuum var. grossum) caused by drechslera bicolor.sweet or bell pepper is a member of the solanaceae family and is regarded as one of the most popular and nutritious vegetable. blight, in the form of leaf and fruit blight, has been observed to infect bell pepper crops cultivated at the horticulture farm in rajasthan college of agriculture, udaipur, india. based on disease severity, we attempted to curb this newly emerged problem using different fungicides, plant extracts, bio-control agents, and commercial botanicals against the fungus in labor ...201727613104
encapsulation of biological and chemical agents for plant nutrition and protection: chitosan/alginate microcapsules loaded with copper cations and trichoderma viride.novel chitosan/alginate microcapsules simultaneously loaded with copper cations and trichoderma viride have been prepared and characterized. information about the intermolecular interactions between biopolymers and bioactive agents was obtained by fourier transform infrared spectroscopy. encapsulation of t. viride spores and the presence of copper cations in the same compartment does not inhibit their activity. microcapsule loading capacity and efficiency as well as swelling behavior and release ...201627715032
exploiting non-conserved residues to improve activity and stability of halothermothrix orenii β-glucosidase.β-glucosidase (ec 3.2.1.21; bg) cleaves β-glucosidic linkages in disaccharide or glucose-substituted molecules. in an effort towards designing better bgs, we focused on the role of non-conserved residues across an otherwise homologous bg active site tunnel and designed mutants across the aglycone-binding site (v169c) and the gatekeeper residues (i246a) of the active site tunnel. we expressed in escherichia coli, the hore_15280 gene encoding a β-glucosidase (bg) in halothermothrix orenii. the ove ...201727761638
tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against mdr bacteria.spherical, rectangular, penta, and hexagonal silver nanoparticles of different dimensions were biosynthesized in an eco-friendly manner by biocontrol agent, trichoderma viride by manipulating physical parameters, ph, temperature, and reaction time. the particles were characterized by uv-vis spectroscopy; dynamic light scattering (dls), transmission electron microscopy (tem) and fourier transform infra-red spectroscopy (ftir). shape and size dependent antimicrobial activity of nanoparticles again ...201727889528
enhanced cellular internalization: a bactericidal mechanism more relative to biogenic nanoparticles than chemical counterparts.biogenic synthesis of silver nanoparticles for enhanced antimicrobial activity has gained a lot of momentum making it an urgent need to search for a suitable biocandidate which could be utilized for efficient capping and shaping of silver nanoparticles with enhanced bactericidal activity utilizing its secondary metabolites. current work illustrates the enhancement of antimicrobial efficacy of silver nanoparticles by reducing and modifying their surface with antimicrobial metabolites of cell free ...201728051856
communication: alamethicin can capture lipid-like molecules in the membrane.alamethicin (alm) is a 19-mer antimicrobial peptide produced by fungus trichoderma viride. above a threshold concentration, alm forms pores across the membrane, providing a mechanism of its antimicrobial action. here we show that at a small concentration which is below the threshold value, alm participates in formation of nanoscale lipid-mediated clusters of guest lipid-like molecules in the membrane. these results are obtained by electron spin echo (ese) technique-a pulsed version of electron p ...201728063425
development of mesorhizobium ciceri-based biofilms and analyses of their antifungal and plant growth promoting activity in chickpea challenged by fusarium wilt.biofilmed biofertilizers have emerged as a new improved inoculant technology to provide efficient nutrient and pest management and sustain soil fertility. in this investigation, development of a trichoderma viride-mesorhizobium ciceri biofilmed inoculant was undertaken, which we hypothesized, would possess more effective biological nitrogen fixing ability and plant growth promoting properties. as a novel attempt, we selected mesorhizobium ciceri spp. with good antifungal attributes with the assu ...201728148979
antifungal activity in seed coat extracts of woodland plants.aqueous extracts from seeds of four woodland ground flora species (hyacinthoides non-scripta, allium ursinum, digitalis purpurea and hypericum pulchrum) were tested for antifungal activity using a petriplate technique. four species of fungi were investigated. the growth of three of these (trichoderma viride, rhizoctonia solani and pythium sp.) was not affected by any of the seed coat extracts. the growth of botrytis cinerea was inhibited by the seed coat extracts of digitalis purpurea and hyperi ...199228313065
effects of different cellulases on the release of phenolic acids from rice straw during saccharification.effects of different cellulases on the release of phenolic acids from rice straw during saccharification were investigated in this study. all cellulases tested increased the contents of phenolic acids during saccharification. however, few free phenolic acids were detected, as they were present in conjugated form after saccharification when the cellulases from trichoderma reesei, trichoderma viride and aspergillus niger were used. on the other hand, phenolic acids were present in free form when t ...201728319769
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