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effects of fibre type and kefir, wine lemon, and pineapple marinades on texture and sensory properties of wild boar and deer longissimus muscle.fibre type percentage and changes in textural parameters, sensory properties as well as mean fibre cross sectional area (csa), fibre shape, endomysium and perimysium thickness of wild boar and deer longissimus (l) muscle subjected to ageing with kefir, dry red wine, lemon and pineapple juice marinades for 4 days were studied. among the non-marinated and non-aged samples of muscles it was found that wild boar meat with its higher percentage of red fibres, higher csa, thicker connective tissue as ...201222766562
light and heavy dansyl reporter groups in food chemistry: amino acid assay in beverages.5-dimethylamino-1-sulfonyl naphthalene (dns, commonly referred as dansyl) is a functionality, bearing well-established properties in directing the fragmentation, by mass spectrometry (ms), of the corresponding ionized sulfonylated derivatives. this property is shared also by its labeled analogs. the use of d(0)/d(6) dns derivatives is now exploited in the application of the well-established isotope dilution mass spectrometric approach in the assay of complex mixtures. a new method for the quanti ...201222791261
development and optimisation by means of sensory analysis of new beverages based on different fruit juices and sherry wine vinegar.despite the long history of sherry wine vinegar, new alternatives of consumption are being developed, with the aim of diversifying its market. several new acetic-based fruit juices have been developed by optimising the amount of sherry wine vinegar added to different fruit juices: apple, peach, orange and pineapple. once the concentrations of wine vinegar were optimised by an expert panel, the aforementioned new acetic fruit juices were tasted by 86 consumers. three different aspects were taken ...201322806566
identification and validation of reference genes to study the gene expression in gluconacetobacter diazotrophicus grown in different carbon sources using rt-qpcr.gluconacetobacter diazotrophicus strain pal5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. currently, g. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens. reverse transcription fol ...201222814372
studies on production of alcoholic beverages from some tropical fruits.the varieties of alcoholic beverages from watermelon; watermelon-banana and watermelon-pineapple mixtures were produced by using monoculture and mixed culture fermentation techniques. three yeast species, namely, saccharomyces cerevisiae, kleochera apicalata, torulospora delbruckii and four bacterial species leuconostoc oenos, lactobacillus sp, micrococcus luteus and streptococcus lactis were identified during the study. the daily succession of these organisms in the various fermenting samples d ...201022815578
palatability, digestibility and emotional pattern in 60 healthy volunteers after ingestion of an iced dessert presented in four different flavours: a subjective evaluation.several variables lead to changes in human and animal eating behaviour and food choices. a pivotal role is played by food palatability, represented by food, smell, taste, texture, appearance and temperature. the aim of our study is to assess the potential differences in palatability and digestibility of four different flavoured iced desserts, consumed at the end of a standardized meal, and their impact on the emotional status of 60 healthy volunteers. sixty healthy volunteers, after ent and psyc ...201522824751
study of the antifungal potential of novel cellulose/copper composites as absorbent materials for fruit juices.cellulose/copper composites with antifungal properties have been synthesized by physical/chemical methods. physical treatments by heat or by a combination of heat and uv radiation provided composites with metallic copper and excellent interfacial adhesion; in contrast, chemical reduction with borohydride generated small although partially aggregated copper oxide nanoparticles. copper micro/nano-particles and copper ions (cu(2+)) were released from the cellulose matrix at an adequate rate to achi ...201222835229
aroma volatile compounds from two fresh pineapple varieties in china.volatile compounds from two pineapples varieties (tainong no.4 and no.6) were isolated by headspace solid phase microextraction (hs-spme) and identified and quantified by gas chromatography-mass spectrometry (gc/ms). in the tainong no. 4 and no. 6 pineapples, a total of 11 and 28 volatile compounds were identified according to their retention time on capillary columns and their mass spectra, and quantified with total concentrations of 1080.44 μg·kg(-1) and 380.66 μg·kg(-1) in the tainong no.4 an ...201222837701
heavy metals health risk assessment for population via consumption of food crops and fruits in owerri, south eastern, nigeria.this study assessed lead, cadmium, and nickel level in food crops, fruits and soil samples from ohaji and umuagwo and owerri in south eastern nigeria and estimated the potential health risks of metals. samples were washed, oven-dried at 70-80°c for 24 h and powdered. samples were digested with perchloric acid and nitric acid. metals were analysed with unicam atomic absorption spectrophotometer.201222853175
effect of mr contrast agents on quantitative accuracy of pet in combined whole-body pet/mr imaging.clinical pet/mr acquisition protocols entail the use of mr contrast agents (mrca) that could potentially affect pet quantification following mr-based attenuation correction (ac). we assessed the effect of oral and intravenous (iv) mrca on pet quantification in pet/mr imaging.201222890801
bromelain limits airway inflammation in an ovalbumin-induced murine model of established asthma.allergic asthma continues to increase despite new pharmacological advances for both acute treatment and chronic-disease management. asthma is a multifactorial disease process with genetic, allergic, infectious, environmental, and dietary origins. researchers are investigating the benefits of lifestyle changes and alternative asthma treatments, including the ability of bromelain to inhibit inflammation. bromelain is a commonly used, proteolytically active pineapple extract.201522894886
a study of lip print pattern in goan dental students - a digital approach.to find the incidence of different types of lip patterns, the dominant pattern, quadrant wise, amongst the goan population. to assess, the quadrant wise differences in lip patterns among males and females and to report new lip print pattern in goan population.201222920761
phenazepam and cannabinomimetics sold as herbal highs in new zealand.a smokeable product called kronic, is legally available, sold under five product names, and marketed in new zealand as containing natural extracts. two such products called pineapple express and purple haze were purchased from shops in auckland city. they were investigated for the presence of synthetic drugs specifically synthesized for recreational purposes. the synthetic cannabinomimetics identified were jwh-018, jwh-073, jwh-122, jwh-250, and 1-pentyl-3-(4-methoxybenzoyl)indol. a compound not ...201222924168
expression patterns, activities and carbohydrate-metabolizing regulation of sucrose phosphate synthase, sucrose synthase and neutral invertase in pineapple fruit during development and ripening.differences in carbohydrate contents and metabolizing-enzyme activities were monitored in apical, medial, basal and core sections of pineapple (ananas comosus cv. comte de paris) during fruit development and ripening. fructose and glucose of various sections in nearly equal amounts were the predominant sugars in the fruitlets, and had obvious differences until the fruit matured. the large rise of sucrose/hexose was accompanied by dramatic changes in sucrose phosphate synthase (sps) and sucrose s ...201222949808
chemical, technological and in vitro antioxidant properties of mango, guava, pineapple and passion fruit dietary fibre concentrate.the aim of this work was to determine the chemical, technological and in vitro antioxidant properties of co-products from the industrialisation of some tropical exotic fruits, such as mango, pineapple, guava and passion fruit, and to evaluate their potential use as dietary fibre sources for food enrichment. proximate compositions were determined, as well as the total, insoluble and soluble fibre contents. the water holding, oil holding and swelling capacities were also determined. for the antiox ...201222953888
detection of postharvest changes of ascorbic acid in fresh-cut melon, kiwi, and pineapple, by using a low cost telemetric system.the present paper deals with a novel telemetric device combined with a carbon amperometric sensor system to determine postharvest changes of ascorbic acid (aa) in fresh-cut fruits, without displacing products out of the storage rooms. the investigation was performed on kiwi, pineapple and melon, subjected to minimal processing, packaging, cold storage, and simulated shelf life. results demonstrated that aa content of fresh-cut fruits of all species declines differently during storage. cold stora ...201222953893
replacement levels of elephant grass by moist pineapple by-product silage in diets of santa inês crossbred sheep: performance and digestibility.the present study evaluated the effect of replacing elephant grass (eg) with moist pineapple by-product silage (ps) on the apparent digestibility, consumption of digestible nutrients and performance of 25 castrated male lambs santa ines crossbreds. the lambs had an initial body weight of 20.2 ± 3.5 kg and were housed in individual pens in a completely randomised design with five treatments (replacement of eg by ps at five proportions of 0, 25, 50, 75 and 100 %) and five replicates during 74 days ...201323001582
pineapple stem bromelain immobilized on different supports: catalytic properties in model wine.bromelain from pineapple stem has been covalently immobilized on different supports to select the more efficient biocatalyst that should be applied toward unstable proteins in real white wine. in this preliminary study, catalytic properties of different immobilized bromelain forms were compared under wine-like conditions, against a synthetic substrate (bz-phe-val-arg-pna).covalent immobilization affected protease kinetic properties, even if all immobilized forms presented both a better substrate ...201223011793
an analysis of consumer demand for fruits in sri lanka. 1981-2010.micronutrient deficiency has become a serious health concern in many countries and sri lanka is no exception. inclusion of vegetables and fruits, which are rich in micronutrients, in the diet is considered as one of the most cost effective measures to alleviate such deficiencies. the purpose of this paper is to analyze consumer demand for fruits in sri lanka. the specific objectives are to: (i) describe the patterns of fruit consumption across various households groups (ii) document the changes ...201323036284
environmental hazards of pesticides from pineapple crop production in the río jiménez watershed (caribbean coast, costa rica).this study aimed to characterize environmental hazards of pesticides from pineapple production in riparian communities along the jiménez river watershed. to achieve our objectives riparian ecological quality indices on riparian habitat and macroinvertebrate assemblages were combined with toxicity assays, fish biomarkers, physico-chemical water analysis and pesticide environmental hazards. during two consecutive years and two periods (july and october), three reference and four impacted sites wer ...201223040047
ethnopharmacological survey of medicinal plants used by indigenous and tribal people in rangamati, bangladesh.there is very limited information regarding plants used by traditional healers in rangamati, bangladesh, for treating general ailments. current study provides significant ethnopharmacological information, both qualitative and quantitative on medical plants in rangamati.201223064284
lc-ms/ms identification of a bromelain peptide biomarker from ananas comosus merr.bromelain (br) is a cysteine peptidase (genbank aeh26024.1) from pineapple, with over 40 years of clinical use. the constituents mediating its anti-inflammatory activity are not thoroughly characterized and no peptide biomarker exists. our objective is to characterize br raw material and identify peptides in the plasma of br treated mice. after sds-page in-gel digestion, br (vn#3507; middletown, ct, usa) peptides were analyzed via lc/ms/ms using 95% protein probability, 95% peptide probability, ...201223082082
de novo assembly, characterization and functional annotation of pineapple fruit transcriptome through massively parallel sequencing.pineapple (ananas comosus var. comosus), is an important tropical non-climacteric fruit with high commercial potential. understanding the mechanism and processes underlying fruit ripening would enable scientists to enhance the improvement of quality traits such as, flavor, texture, appearance and fruit sweetness. although, the pineapple is an important fruit, there is insufficient transcriptomic or genomic information that is available in public databases. application of high throughput transcri ...201223091603
design and development of batch type acetifier for wine-vinegar production.a batch type acetifier based on the principal of acetic acid fermentation was designed and tested for production of wine-vinegar from the pineapple peel waste. the pineapple peels along with starter solution was fed to the inner ss perforated peel-solid separator tank 130 mm dia having perforations of 50 mm size. the concentric perforated peel-solid separator circular tank was fitted inside the collecting tank having 255 mm dia. the pineapple peels and starter solution in perforated peel-solid s ...200723100658
rna interference of endochitinases in the sugarcane endophyte trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.the sugarcane root endophyte trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including n-acetyl-ß-d-glucosaminidases, chitobiosidases and endochitinases. we used targeted gene deletion and rna-dependent gene silencing strategies to disrupt n-acetyl-ß-d-glucosaminidase and endochitinase activities of the ...201223110120
serum melatonin levels and antioxidant capacities after consumption of pineapple, orange, or banana by healthy male volunteers.melatonin is a naturally occurring molecule biosynthesized by the pineal gland of vertebrates; it also has been identified in many plants. it is considered an important antioxidant and may retard the development of some neurodegenerative diseases and cancer. previous studies in humans have measured melatonin metabolites in urine and have indicated that melatonin-containing foods may provide dietary melatonin. this study tested whether the consumption of fruits or fruit juice containing melatonin ...201323137025
solution structure of a phytocystatin from ananas comosus and its molecular interaction with papain.the structure of a recombinant pineapple cystatin (accys) was determined by nmr with the rmsd of backbone and heavy atoms of twenty lowest energy structures of 0.56 and 1.11 å, respectively. it reveals an unstructured n-terminal extension and a compact inhibitory domain comprising a four-stranded antiparallel β-sheet wrapped around a central α-helix. the three structural motifs (g(45), q(89)xvxg, and w(120)) putatively responsible for the interaction with papain-like proteases are located in one ...201223139757
design development scopes towards occupational wellness of women workers: specific reference to local agro based food processing industries in ne india.women workers constitute one of the most vulnerable segments of the country's labour force. they often face different workplace health challenges than men do. they are engaged in a range of work that extends from heavy, monotonous, repetitive jobs, which are in many times experienced with low-paid and involves in long hours of work. women's workplace health problems are frequently compounded by getting more of the same at home--the "double jeopardy" of domestic work. specific issues to improve t ...201223151732
biodegradation of selected nigerian fruit peels by the use of a non-pathogenic rhizobium species cwp g34b.this study was carried out to determine the ability of rhizobium species cwp g34b to degrade the peels of selected nigerian fruits. the potential of the bacterium to digest some carbon sources (lactose, maltose, sucrose and mannitol) and peels of some nigerian fruits (pineapple, orange, plantain, banana, pawpaw and mango fruits) was investigated by growing the organism on the substances separately after which dnsa reagent method was used to quantify glucose released into the medium. the results ...201223166567
headspace solid-phase microextraction gas chromatography-mass spectrometry and gas chromatography-olfactometry analysis of volatile compounds in pineapple breads.sensorial analysis of pineapple breads (conventionally baked, cpb; fully baked frozen, fpb and partially baked, ppb) showed no significant differences in terms of aroma and taste. on the contrary, the scores for the overall quality between the partially baked and conventionally baked breads showed significant (p < 0.05) differences. at the same time, headspace analysis using a solid-phase microextraction (spme) method identified 59 volatile compounds. the results of the aroma extracts dilution a ...201223174897
food allergy in africa: myth or reality?food allergy has been traditionally perceived as being rare in africa. however, the prevalence of other allergic manifestations such as asthma and atopic dermatitis continue to rise in the higher-income african countries. since the food allergy epidemic in westernized countries has lagged behind that of allergic respiratory conditions, we hypothesize that food allergy is increasing in africa. this article systematically reviews the evidence for food allergy in africa, obtained through searching ...201423179518
agrobacterium-mediated genetic transformation of pineapple (ananas comosus l., merr.).pineapple (ananas comosus l., merr.) is a commercially important crop, grown in the tropical and subtropical regions. however, the crop is faced with postharvest damage and poor varietal and nutritional improvement. being a vegetatively propagated crop, conventional breeding programs take longer time for genetic improvement, which may not necessarily successfully develop an improved cultivar. hence, the genetic modification of pineapple is an alternative handy approach to improve pineapple. we h ...201323179718
comparison of activities and properties of pyrophosphate and adenosine triphosphate-dependent phosphofructokinases of black gram (phaseolus mungo) seeds.both pyrophosphate-dependent phosphofructokinase (ppi-pfkase, ec 2.7.1.90) and atpdependent phosphofructokinase (atp-pfkase, ec 2.7. 1.11) were present in dry and germinated black gram seeds. in the absence of fructose-2,6-biphosphate (f2,6bp), the activity of ppi-pfkase expressed as nmol · min(-1) · (pair of cotyledons)(-1) was much lower than that of atp-pfkase in both dry and germinated seeds. however, ppi-pfkase was activated by f2,6bp and its activity reached the same level as atp-pfkase ac ...198423195152
synthesis, characterization and adsorptive properties of carbon with iron nanoparticles and iron carbide for the removal of as(v) from water.this manuscript presents the synthesis of carbon modified with iron nanoparticles (cfe) and iron carbide (carfe) from the pyrolyzed crown leaves of pineapple (ananas comosus) treated with iron salts. the materials that were obtained were used for the removal of as(v) from aqueous media. the carbonaceous materials were characterized by scanning electron microscopy (sem), transmission electron microscopy (tem), x-ray diffraction (xrd), x-ray photoelectron spectroscopy (xps) and mossbauer spectrosc ...201323201599
anti-inflammatory properties of fruit juices enriched with pine bark extract in an in vitro model of inflamed human intestinal epithelium: the effect of gastrointestinal digestion.enrichment of fruit juices with pine bark extract (pbe) could be a strategy to compensate for phenolic losses during the gastrointestinal digestion. a coculture system with caco-2 cells and raw 264.7 macrophages was established as an in vitro model of inflamed human intestinal epithelium for evaluating the anti-inflammatory capacity of fruit juices enriched with pbe (0.5 g l(-1)) before and after in vitro digestion. the digestion of both pbe-enriched pineapple and red fruit juice led to signific ...201323220608
inhibitory effects of a kunitz-type inhibitor from pithecellobium dumosum (benth) seeds against insect-pests' digestive proteinases.pithecellobium dumosum is a tree belonging to the mimosoideae subfamily that presents various previously characterized kunitz-type inhibitors. the present study provides a novel kunitz-trypsin inhibitor isoform purified from p. dumosum seeds. purification procedure was performed by tca precipitation followed by a trypsin-sepharose chromatography and a further reversed-phase hplc. purified inhibitor (pdki-4) showed enhanced inhibitory activity against bovine trypsin and chymotrypsin. furthermore, ...201323238511
microarray analysis of gene expression profiles in ripening pineapple fruits.pineapple (ananas comosus) is a tropical fruit crop of significant commercial importance. although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. in this study we developed a 9277 element pineapple microa ...201223245313
dietary intake of melatonin from tropical fruit altered urinary excretion of 6-sulfatoxymelatonin in healthy volunteers.this study assessed the melatonin content of six tropical fruits and examined whether human consumption could contribute to dietary melatonin as measured by 6-sulfatoxymelatonin (amt6-s, a marker of circulating melatonin in the body). melatonin was extracted using methanol and analyzed by high-performance liquid chromatography. in a clinical crossover study, 30 healthy volunteers consumed selected fruits one at a time, with a 1week wash-out period between fruits, until completing all six fruits. ...201323252791
pineapple translation factor sui1 and ribosomal protein l36 promoters drive constitutive transgene expression patterns in arabidopsis thaliana.the availability of a variety of promoter sequences is necessary for the genetic engineering of plants, in basic research studies and for the development of transgenic crops. in this study, the promoter and 5' untranslated regions of the evolutionally conserved protein translation factor sui1 gene and ribosomal protein l36 gene were isolated from pineapple and sequenced. each promoter was translationally fused to the gus reporter gene and transformed into the heterologous plant system arabidopsi ...201323263857
properties and therapeutic application of bromelain: a review.bromelain belongs to a group of protein digesting enzymes obtained commercially from the fruit or stem of pineapple. fruit bromelain and stem bromelainare prepared differently and they contain different enzymatic composition. "bromelain" refers usually to the "stem bromelain." bromelain is a mixture of different thiol endopeptidases and other components like phosphatase, glucosidase, peroxidase, cellulase, escharase, and several protease inhibitors. in vitro and in vivo studies demonstrate that ...201223304525
mycotoxins biosynthesized by plant-derived fusarium isolates.there is little information on secondary metabolites produced by fusaria infecting crop plants other than cereals. many members of fusarium genus have the ability to colonise perennial crops with only scarce infection or disease symptoms or with no symptoms at all while still being detectable. even in case of such asymptomatic infection, significant mycotoxin contamination of the plant tissues is possible. the aim of this study was to characterise the spectrum of fusarium species isolates obtain ...201223334038
effect of acid fruit juices combined with electric or sonic toothbrushing on root dentin permeability--an in vitro study.the purpose of this in vitro study was to quantify the alterations on human root dentin permeability after exposure to different acid fruit juices and to evaluate the effect of toothbrushing with electric or sonic toothbrush after acid exposure. the root dentin of 50 extracted third molars was exposed with a high speed bur. crowns were sectioned above the cementoenamel junction and root fragments were used to prepare dentin specimens. specimens were randomly assigned to 5 groups according to the ...201223338258
dna profiling of pineapple cultivars in japan discriminated by ssr markers.we developed 18 polymorphic simple sequence repeat (ssr) markers in pineapple (ananas comosus) by using genomic libraries enriched for ga and ca motifs. the markers were used to genotype 31 pineapple accessions, including seven cultivars and 11 breeding lines from okinawa prefecture, 12 foreign accessions and one from a related species. these ssr loci were highly polymorphic: the 31 accessions contained three to seven alleles per locus, with an average of 4.1. the values of expected heterozygosi ...201223341750
anti-inflammatory activity of bromelia hieronymi: comparison with bromelain.some plant proteases (e. g., papain, bromelain, ficin) have been used as anti-inflammatory agents for some years, and especially bromelain is still being used as alternative and/or complementary therapy to glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. bromelain is an extract rich in cysteine endopeptidases obtained from ananas comosus. in this study the anti-inflammatory action of a partially purified extract of bromelia hieronymi fruits, whose main components are cysteine ...201323364884
changes in vitamin c, phenolic, and carotenoid profiles throughout in vitro gastrointestinal digestion of a blended fruit juice.the aim of this research was to evaluate the influence of an in vitro gastrointestinal digestion on the stability and bioaccessibility of vitamin c, phenolic compounds, and carotenoids, as well as the antioxidant activity in a blended fruit juice (bfj) containing orange, pineapple, and kiwi. vitamin c and most of the analyzed phenolic compounds were quite stable under gastric conditions (recovery > 75%), whereas carotenoids diminished significantly (to 64%). the concentration of all the evaluate ...201323374081
reverse zymography alone does not confirm presence of a protease inhibitor.reverse zymography is applied for identification and semi-quantification of protease inhibitors that are of protein in nature. however, a protein that shows band in reverse zymography against a protease used for digestion of the gel need not be an inhibitor; it might be resistant to degradation by the protease. we demonstrate that in reverse zymography, avidin, streptavidin and the leaf extract of catharanthus roseus behave like inhibitors of proteases like papain, ficin, bromelain extracts from ...201323417772
physicochemical and antioxidant properties of malaysian honeys produced by apis cerana, apis dorsata and apis mellifera.the aim of the present study was to evaluate the physicochemical and antioxidant properties of malaysian monofloral honey samples-acacia, pineapple and borneo honey-and compare them with tualang honey. acacia and pineapple honey are produced by apis mellifera bees while borneo and tualang honey are produced by apis cerana and apis dorsata bees, respectively.201323433009
the use of near infrared spectroscopy and multivariate techniques to differentiate escherichia coli and salmonella enteritidis inoculated into pulp juice.this study shows the application and usefulness of near infrared (nir) transflectance spectra measurements in the identification and classification of escherichia coli and salmonella enteritidis from commercial fruit pulp (pineapple). principal component analysis (pca), soft independent modeling of class analogy (simca) analysis and partial least-squares discriminant analysis (pls-da) were used in the analysis. it was not possible to obtain total separation between the samples using pca and simc ...201323470963
the concept of the eudicot shoot apical meristem as it applies to four spiraea (rosaceae), one mentha (lamiaceae) and one euonymus (celastraceae) cultivars based on chimeric analysis.eversporting eudicots were sought to see if they behave like gymnosperms. behaviour of eversporting gymnosperm chimeras indicates a single apical cell is present in sam and it would be of interest to see if eudicot chimeras have the same behaviour.201323482330
evaluation of the odour of aminoleban(®) en, taste-masked with flavoured powders, by human and electronic noses.aminoleban® en is a bitter total enteral nutrient product with an unpleasant odour. the purpose of this study was to evaluate the odour of aminoleban® en, taste-masked with various flavoured powders, by human testing and by using an electronic nose system (alpha m.o.s.).201223488778
in vitro activity of pineapple extracts (ananas comosus, bromeliaceae) on rhipicephalus (boophilus) microplus (acari: ixodidae).measures to control the cattle tick, rhipicephalus (boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. the objective of this study was to test the effect of the aqueous extract of pineapple skin (aeps) and bromelain extracted from the stem (sigma-aldrich®, b4882) on engorged females and larvae of r. (b.) microplus in vitro. these substances were diluted in water and evaluated at eight concentrations. engorged females ...201323541882
structural characterization of a glucuronoarabinoxylan from pineapple (ananas comosus (l.) merrill) gum exudate.native polysaccharide from pineapple gum (panp) was obtained following alkaline extraction of gum and fractionation with cetylpyridinium chloride. it was characterized as a glucuronoarabinoxylan using nmr, methylation data, controlled smith degradation, carboxy-reduction, and esi-ms of oligosaccharides produced on mild acid hydrolysis of panp. hspec-malls-ri of carboxy-reduced fraction showed homogeneous profile (mw 1.943×10(5) g/mol). panp was composed of ara, xyl, gal, and glcpa (40:23:7:30 mo ...201323544593
sonicated pineapple juice as substrate for l. casei cultivation for probiotic beverage development: process optimisation and product stability.the aim of this study was to evaluate the use of sonicated pineapple juice as substrate for producing a probiotic beverage by lactobacillus casei nrrl b442. maximal microbial viability was found by cultivating l. casei at 31°c and ph 5.8 (optimised conditions). after fermentation, samples of sweetened and non-sweetened juice were stored. after 42 days of storage under refrigeration (4°c), the microbial viability was 6.03 log cfu/ml in the non-sweetened sample and 4.77 log cfu/ml in the sweetened ...201323561104
modulatory effect of pineapple peel extract on lipid peroxidation, catalase activity and hepatic biomarker levels in blood plasma of alcohol-induced oxidative stressed rats.to investigate the ability of the methanolic extract of pineapple peel to modulate alcohol-induced lipid peroxidation, changes in catalase activities and hepatic biochemical marker levels in blood plasma.201123569717
diversity of fusarium species and mycotoxins contaminating pineapple.pineapple (ananas comosus var. comosus) is an important perennial crop in tropical and subtropical areas. it may be infected by various fusarium species, contaminating the plant material with mycotoxins. the aim of this study was to evaluate fusarium species variability among the genotypes isolated from pineapple fruits displaying fungal infection symptoms and to evaluate their mycotoxigenic abilities. forty-four isolates of ten fusarium species were obtained from pineapple fruit samples: f. ana ...201323572446
efficacy of xylanase purified from aspergillus niger dfr-5 alone and in combination with pectinase and cellulase to improve yield and clarity of pineapple juice.pineapple is one of the fruits having xylan rich hemicellulose content more than pectin. therefore, the efficacy of absolutely purified xylanase from a. niger dfr-5 alone and in combination with pectinase and cellulase on juice yield and clarity was studied. xylanase provided maximum yield (71.3%) and clarity (64.7%) of juice in comparison to control responses (61.8% yield and 57.8% clarity). when used together, a synergistic effect of xylanase, pectinase and cellulase on process responses was o ...201123572788
cytotoxic effects of bromelain in human gastrointestinal carcinoma cell lines (mkn45, kato-iii, ht29-5f12, and ht29-5m21).bromelain is a pineapple stem extract with a variety of therapeutic benefits arising from interaction with a number of different biological processes. several preclinical studies and anecdotal clinical observations have reported the anticancer properties of bromelain. in the present study, we investigated the cytotoxic effects of bromelain in four human cancer cell lines of gastrointestinal origin and the mechanisms involved.201323620673
zinc absorption in brazilian subjects fed a healthy meal.the high amounts of phytic acid present in diets from developing countries are considered as important inhibitors of zinc (zn) absorption. the present study aimed to assess the fractional absorption of zn from a meal containing common brazilian foods using the stable isotope technique.201423634993
production of laccase and manganese peroxidase by pleurotus pulmonarius in solid-state cultures and application in dye decolorization.the production of ligninolytic enzymes (laccase and mn-dependent peroxidase) by the white-rot fungus pleurotus pulmonarius (fr.) quélet was studied in solid-state cultures using agricultural and food wastes as substrate. the highest activities of laccase were found in wheat bran (2,860 ± 250 u/l), pineapple peel (2,450 ± 230 u/l), and orange bagasse (2,100 ± 270 u/l) cultures, all of them at an initial moisture level of 85 %. the highest activities of mn peroxidase were obtained in pineapple pee ...201323645502
pineapple juice ingestion for gastric discomfort in diabetic gastroparesis. 201323650790
is pomegranate juice a potential perpetrator of clinical drug-drug interactions? review of the in vitro, preclinical and clinical evidence.the area of fruit juice-drug interaction has received wide attention with numerous scientific and clinical investigations performed and reported for scores of drugs metabolized by cyp3a4/cyp2c9. while grapefruit juice has been extensively studied with respect to its drug-drug interaction potential, numerous other fruit juices such as cranberry juice, orange juice, grape juice, pineapple juice and pomegranate juice have also been investigated for its potential to show drug-drug interaction of any ...201323673492
in vitro and in vivo evaluation of the activity of pineapple (ananas comosus) on haemonchus contortus in santa inês sheep.the development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. this study aimed to assess the activity of ananas comosus on haemonchus contortus in santa inês sheep. the aqueous extract of pineapple skin (aeps), bromelain from pineapple stems (b4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. the enzymatic activity of substances was analyzed by the azocasein method. the egg ha ...201323688638
factors significantly increasing or inhibiting early stages of malignant melanoma (m.m.) and non-invasive evaluation of new treatment by ingestion and external application of optimal doses of the most effective anti-m.m. substances: haritaki, cilantro, vitamin d3, nori, epa with dha, & application of special (+) solar energy stored paper, which reduced the m.m. active area & asbestos rapidly.sterilizing the pre-cancer skin of malignant melanoma (m.m.) with 70% isopropyl alcohol intensified malignancy & the malignant response extended to surrounding normal looking skin, while sterilizing with 80% (vodka) or 12% (plum wine) ethyl alcohol completely inhibited m.m. in the area (both effects lasted for about 90 minutes initially). burnt food (bread, vegetables, meat, and fish), a variety of smoked & non-smoked fish-skin, many animal's skin, pepper, vitamin c over 75 mg, mango, pineapple, ...201323724697
the influence of total solids content and initial ph on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes.hydrogen is a valuable clean energy source, and its production by biological processes is attractive and environmentally sound and friendly. in méxico 5 million tons/yr of agroindustrial wastes are generated; these residues are rich in fermentable organic matter that can be used for hydrogen production. on the other hand, batch, intermittently vented, solid substrate fermentation of organic waste has attracted interest in the last 10 years. thus the objective of our work was to determine the eff ...201323732191
antibacterial activity of selected malaysian honey.antibacterial activity of honey is mainly dependent on a combination of its peroxide activity and non-peroxide components. this study aims to investigate antibacterial activity of five varieties of malaysian honey (three monofloral; acacia, gelam and pineapple, and two polyfloral; kelulut and tualang) against staphylococcus aureus, bacillus cereus, escherichia coli, and pseudomonas aeruginosa.201323758747
bromelain down-regulates myofibroblast differentiation in an in vitro wound healing assay.bromelain, a pineapple-derived enzyme mixture, is a widely used drug to improve tissue regeneration. clinical and experimental data indicate a better outcome of soft tissue healing under the influence of bromelain. proteolytic, anti-bacterial, anti-inflammatory, and anti-oedematogenic effects account for this improvement on the systemic level. it remains unknown, whether involved tissue cells are directly influenced by bromelain. in order to gain more insight into those mechanisms by which brome ...201323771413
the expression patterns of bromelain and accys1 correlate with blackheart resistance in pineapple fruits submitted to postharvest chilling stress.blackheart is a physiological disorder induced by postharvest chilling storage during pineapple fruit export shipping. the aim of this study was to check the involvement of bromelain, the cysteine protease protein family abundantly present in pineapple fruits, and accys1, an endogenous inhibitor of bromelain, in the development of blackheart. for this we checked the response to postharvest chilling treatment of two pineapple varieties (md2 and smooth cayenne) differing in their resistance to bla ...201323777839
using choice experiments to understand household tradeoffs regarding pineapple production and environmental management in costa rica.choices among environmental management alternatives involve tradeoffs where, for example, the benefits of environmental protection may be offset by economic costs or welfare losses to individual agents. understanding individual or household-level preferences regarding these tradeoffs is not always straightforward, and it often requires an analysis of choices under alternative scenarios. a household survey was used to gather data for a choice experiment, where respondents were asked to choose amo ...201323807434
deconstructing a fruit serving: comparing the antioxidant density of select whole fruit and 100% fruit juices.research suggests phytonutrients, specifically phenolic compounds, within fruit may be responsible for the putatively positive antioxidant benefits derived from fruit. given the prominence of fruit juice in the american diet, the purpose of this research was to assess the antioxidant density of fresh fruit and 100% fruit juice for five commonly consumed fruits and juices and to compare the adequacy of 100% juice as a dietary equivalent to whole fruit in providing beneficial antioxidants. antioxi ...201323810279
a novel aspartic acid protease gene from pineapple fruit (ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance.a full-length cdna encoding a putative aspartic acid protease (acap1) was isolated for the first time from the flesh of pineapple (ananas comosus) fruit. the deduced sequence of acap1 showed all the common features of a typical plant aspartic protease phytepsin precursor. analysis of acap1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. the resistant variety showed an up-regulation o ...201323838125
in vitro bacterial fermentation of tropical fruit fibres.tropical fruits such as mango, papaya, pineapple and banana are rich sources of dietary fibre. however, few studies have examined the potential physiological effects of fibre from these tropical fruits. the aim of this study was to characterise the fermentability of dietary fibre found in banana, papaya, pineapple and mango as an estimate of the physiological effects of consuming these fruits. freeze-dried fruit was subjected to in vitro digestion to remove digestible carbohydrates. digestion re ...201323887033
pectin methylesterase of datura species, purification, and characterization from datura stramonium and its application.pectin methylesterases (pme; ec 3.1.1.11) involved in de-esterification of pectin and have applicability in food, textiles, wines, pulp, and paper industries. in the present study, we compared pme activity of different parts of 3 datura species and found that fruit coat showed maximum pme activity followed by leaf and seed. pme from leaves of d. stramonium (dspme) was purified and characterized. dspme showed optimum activity at 60 °c and ph 9 in the presence of 0.3 m nacl. dspme was stable at 70 ...201323887498
color and odor of artificial fruit used to signal potential dispersers in the atlantic forest in brazil.fruit color and odor are the main features regulating the rate of fruit predation and dispersal. the aim of this study was to analyze the effect of odor and color on fruit predators and dispersers. the present study was conducted in a 30ha area of secondary forest in southeastern atlantic brazil. this area was divided into two transects, in which four points were marked with a 30m distance from each other. each sampling point contained a total of 30 artificial fruit which belong to six different ...201223894956
transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.citrus canker provoked by xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. xanthomonas infection is considered as an endemic disease in northeast and northwest argentina, affecting as much as 10% of commercial citrus plantations. there is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. to introduce resistance to this disease, plants of pineapple sweet ...201323896218
serving a variety of vegetables and fruit as a snack increased intake in preschool children.although serving a greater variety of food increases intake, this effect has not been well studied as a strategy to encourage consumption of vegetables and fruit in preschool children.201323902783
functional characterization of recombinant bromelain of ananas comosus expressed in a prokaryotic system.bromelain (brm) is a defense protein present in the fruit and stem of pineapple (ananas comosus) and it is grouped as a cysteine protease enzyme with diversified medicinal uses. based on its therapeutic applications, bromelain has got sufficient attention in pharmaceutical industries. in the present study, the full coding gene of bromelain in pineapple stem (1,093 bp) was amplified by rt-pcr. the pcr product was cloned, sequenced, and characterized. the sequence analysis of the gene revealed the ...201423921698
an in vitro study reveals nutraceutical properties of ananas comosus (l.) merr. var. mauritius fruit residue beneficial to diabetes.rapid urbanisation and nutritional transition is fuelling the increased global incidence of type 2 diabetes. pineapple fruit residue was explored for its nutraceutical properties as an alternative or adjunct to currently available treatment regime. ethyl acetate and methanolic extracts of pineapple fruit residue were evaluated for anti-diabetic activity in cell free and cell based systems. specifically, we assessed: (1) antioxidant potential, (2) anti-glycation potential, (3) carbohydrate digest ...201423929507
fruit juice drinks prevent endogenous antioxidant response to high-fat meal ingestion.high-fat meals (hfm) induce metabolic stress, leading to the activation of protective mechanisms, including inflammation and endogenous antioxidant defences. in the present study, we investigated the effects of antioxidant-rich fruit juice drinks on the endogenous antioxidant response induced by hfm. in a double-blind, cross-over design (10 d washout), fourteen overweight volunteers were randomly assigned to one of the following interventions: hfm+500 ml placebo beverage (hfm-pb, free from fruit ...201423930843
optimized butyl butyrate synthesis catalyzed by thermomyces lanuginosus lipase.butyl butyrate is an ester present in pineapple flavor, which is very important for the food and beverages industries. in this work, the optimization of the reaction of butyl butyrate synthesis catalyzed by the immobilized lipase lipozyme tl-im was performed. n-hexane was selected as the most appropriate solvent. other reaction parameters such as temperature, substrate molar ratio, biocatalyst content and added water, and their responses measured as yield, were evaluated using a fractional facto ...201323946156
adding pineapple juice to a polyethylene glycol-based bowel cleansing regime improved the quality of colon cleaning.an unsuccessful colonoscopy procedure is often related to inadequate bowel cleansing. it is difficult for patients to finish the whole 4 liters of polyethylene glycol-electrolyte lavage (peg-el) because of its salty taste and the large quantity. pineapple juice has been shown to be an effective agent in the dissolution of undigested food in the stomach. this study assessed the effectiveness of both 2 and 4 liters of peg-el in precolonoscopic bowel cleansing and the quality of colonoscopic cleani ...201323949576
the physiology of ex vitro pineapple (ananas comosus l. merr. var md-2) as cam or c3 is regulated by the environmental conditions: proteomic and transcriptomic profiles.proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under c3 and cam-inducing metabolisms to obtain insight into the cam-facultative metabolism and the relationship of cam plants with oxidative stress.201323959598
screening of trichoderma isolates as a biological control agent against ceratocystis paradoxa causing pineapple disease of sugarcane.in this study, dual culture, poison agar, and direct methods were used to assess the ability of trichoderma virens imi-392430, t. pseudokoningii imi-392431, t. harzianum imi-392432, t. harzianum imi-392433, and t. harzianum imi-392434 to control ceratocystis paradoxa, which causes the pineapple disease of sugarcane. the highest percentage inhibition of radial growth (pirg) values were observed with t. harzianum imi-392432 using two dual culture methods, 63.80% in method i and 80.82% in method ii ...200923983548
phylogeny and classification of dickeya based on multilocus sequence analysis.bacterial heart rot of pineapple reported in hawaii in 2003 and reoccurring in 2006 was caused by an undetermined species of dickeya. classification of the bacterial strains isolated from infected pineapple to one of the recognized dickeya species and their phylogenetic relationships with dickeya were determined by a multilocus sequence analysis (mlsa), based on the partial gene sequences of dnaa, dnaj, dnax, gyrb and recn. individual and concatenated gene phylogenies revealed that the strains f ...201324003072
[vitamin c in fruits and vegetables].strong opinion about reducing vitamin c content in traditional cultivars of fruits and vegetables as a result of intensive farming practices, on the one hand, and depletion of soil, waste of fertilizers, on the other hand, takes place. the aim of the study was to assess changes in vitamin c content in fresh vegetables, fruits and berries from the 40s of last century to the present. available national and foreign data from official tables of the chemical composition tables published in different ...201324006751
activation of words with phonological overlap.multiple lexical representations overlapping with the input (cohort neighbors) are temporarily activated in the listener's mental lexicon when speech unfolds in time. activation for cohort neighbors appears to rapidly decline as soon as there is mismatch with the input. however, it is a matter of debate whether or not they are completely excluded from further processing. we recorded behavioral data and event-related brain potentials (erps) in auditory-visual word onset priming during a lexical d ...201324009593
errors and myths in feeding and nutrition: impact on the problems of obesity.the increase in obesity prevalence cannot be explained by a sudden and generalized change in human genome. it is certainly due to the modification of lifestyle habits and especially of the diet, as well as a lack of physical activity and sedentary living. changes in the feeding pattern and the subsequent unbalance in the caloric profile of the diet may have had great importance in the occurrence of obesity. the social pressure in relation to the body image, the desire to have a slim body, and th ...201324010747
polymer-based alternative method to extract bromelain from pineapple peel waste.bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (ananas comosus merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. the use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. we aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-ph ...201624011234
development of pineapple microsatellite markers and germplasm genetic diversity analysis.two methods were used to develop pineapple microsatellite markers. genomic library-based ssr development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8%) of the 94 simple sequence repeat (ssr) loci were dinucleotide repeats (39 ac/gt repeats and 52 ga/tc repeats, accounting for 42.9% and 57.1%, resp.), and the other three were mononucleotide repeats. thirty-six pairs of ssr primers were designed; 24 of them generated clear ...201324024187
antimicrobial activity and potential use of monoterpenes as tropical fruits preservatives.banana, papaya and pineapple are the most consumed tropical fruits in the world, being brazil one of the main producers. fungi colletotrichum musae, colletotrichum gloeosporioides and fusarium subglutinans f.sp. ananas cause severe post harvest diseases and losses in fruits quality. the aim of this work was to evaluate the effectiveness of five monoterpenes to inhibit the mycelial growth and conidia germination of these three phytopathogens. the monoterpenes citral, citronellal, l-carvone, isopu ...200824031197
structural interaction between gfp-labeled diazotrophic endophytic bacterium herbaspirillum seropedicae ram10 and pineapple plantlets 'vitória'.the events involved in the structural interaction between the diazotrophic endophytic bacterium herbaspirillum seropedicae, strain ram10, labeled with green fluorescent protein, and pineapple plantlets 'vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. after 6 hours of inoculation, h. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epiderm ...201124031612
growth of byssochlamys nivea in pineapple juice under the effect of water activity and ascospore age.the study of thermal resistant mould, including byssochlamys nivea, is of extreme importance since it has been associated with fruit and fruit products. the aim of this work is to analyze the influence of water activity (aw) and ascospore age (i) on the growth of byssochlamys nivea in pineapple juice. mold growth was carried out under different conditions of water activity (aw) (0.99, 0.96, 0.95, 0.93, 0.90) and ascospore age (i) (30, 51, 60, 69, 90 days). growth parameters as length of adaptati ...201124031622
highly thermostable and ph-stable cellulases from aspergillus niger ns-2: properties and application for cellulose hydrolysis.optimization of cultural conditions for enhanced cellulase production by aspergillus niger ns-2 were studied under solid-state fermentation. significant increase in yields (cmcase 463.9 ± 20.1 u/g, fpase 101.1 ± 3.5 u/g and β-glucosidase 99 ± 4.0 u/g) were obtained under optimized conditions. effect of different nutritional parameters was studied to induce the maximum production of cellulase complex. scale-up studies for enzyme production process were carried out. characterization studies showed ...201424052336
identification of transcription factors potentially involved in the juvenile to adult phase transition in citrus.the juvenile to adult transition (jat) in higher plants is required for them to reach reproductive competence. however, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. this work aims at increasing our understanding of the mechanisms regulating the jat in citrus.201324052558
quantification of bioactive compounds in pulps and by-products of tropical fruits from brazil.this study aimed to quantify the levels of resveratrol, coumarin, and other bioactives in pulps and by-products of twelve tropical fruits from brazil obtained during pulp production process. pineapple, acerola, monbin, cashew apple, guava, soursop, papaya, mango, passion fruit, surinam cherry, sapodilla, and tamarind pulps were evaluated as well as their by-products (peel, pulp's leftovers, and seed). total phenolic, anthocyanins, yellow flavonoids, β-carotene and lycopene levels were also deter ...201424054258
separation of a binary mixture of pesticides in fruits using a flow-through optosensor.a flow-through optosensor is here proposed for the determination of mixtures of two widely used pesticides, carbendazim and o-phenylphenol, in fruits. the pesticides are separated on-line using an additional amount of solid support, c18 silica gel, in the flow-through cell. the resolution is performed due to the different retention/desorption kinetics of the analytes when interacting with the c18 microbeads. therefore, both separation and determination are integrated in the same cell, considerab ...201324054619
enzymatic, antimicrobial and toxicity studies of the aqueous extract of ananas comosus (pineapple) crown leaf.various parts of the plant pineapple (ananas comosus) are used in traditional medicine worldwide for treatment of a number of diseases and disorders. in folk medicine, pineapple leaf extract was used as an antimicrobial, vermicide, purgative, emmenagoogue, abortifacient, anti-oedema and anti-inflammatory agent. compared to the fruit and stem extracts of pineapple, information about its leaf extract is limited. the potential of pineapple crown leaf extract as an ethno-medicine has been evaluated ...201324076462
the chemopreventive action of bromelain, from pineapple stem (ananas comosus l.), on colon carcinogenesis is related to antiproliferative and proapoptotic effects.colorectal cancer is an important health problem across the world. here, we investigated the possible antiproliferative/proapoptotic effects of bromelain (from the pineapple stem ananas comosus l., family bromeliaceae) in a human colorectal carcinoma cell line and its potential chemopreventive effect in a murine model of colon cancer.201424123777
the relative anthelmintic efficacy of plant-derived cysteine proteinases on intestinal nematodes.we examined the in vitro and in vivo efficacy of plant cysteine proteinases (cps) derived from pineapple (ananas comosus) and kiwi fruit (actinidia deliciosa), and compared their efficacy as anthelmintics to the known effects of cps from the latex of papaya (carica papaya) against the rodent intestinal nematode, heligmosomoides bakeri. both fruit bromelain and stem bromelain had significant in vitro detrimental effects on h. bakeri but in comparison, actinidain from kiwi fruit had very little ef ...201524176056
comparison of biofilm and attachment mechanisms of a phytopathological and clinical isolate of klebsiella pneumoniae subsp. pneumoniae.some bacterial species can colonize humans and plants. it is almost impossible to prevent the contact of clinically pathogenic bacteria with food crops, and if they can persist there, they can reenter the human food chain and cause disease. on the leaf surface, microorganisms are exposed to a number of stress factors. it is unclear how they survive in such different environments. by increasing adhesion to diverse substrates, minimizing environmental differences, and providing protection against ...201324222755
production of pineapple plants in vitro.in vitro culture of pineapple (ananas comosus) was studied to determine the efficiency of axillary bud culture for rapid propagation of several cultivars. the technique used maximizes the success rate of various steps in the production of pineapple plants. rapid mass multiplication of plantlets started 9 months after explanting with a significant log phase. the number of plantlets obtained from the culture of a single bud by the thirteenth month ranged from 210 to 380 for 'perolera'; 300 to 350 ...198824240410
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