Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [genetic effect of panicle traits of intersubspecific crosses of rice (oryza sativa.) and its interaction with environment]. | the models of genetic effects and genotype x environment interaction for additive-dominant-additive x additive epistasis were used to analyze the panicle traits of intersubspecific crosses of rice (oryza sativa.) in different environments. it was found that significant additive, dominant and additive x additive epistatic effects and genotype x environment (ge) interaction were observed in main panicle length, spikelet density, primary branches, total lengths of primary and secondary branches, bu ... | 2000 | 11766595 |
| nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types. | nonsymbiotic hemoglobins (ns-hbs) previously have been found in monocots and dicots; however, very little is known about the tissue and cell type localization as well as the physiological function(s) of these oxygen-binding proteins. we report the immunodetection and immunolocalization of ns-hbs in rice (oryza sativa l.) by western blotting and in situ confocal laser scanning techniques. ns-hbs were detected in soluble extracts of different tissues from the developing rice seedling by immunoblot ... | 2001 | 11770429 |
| rice (oryza sativa) contains a novel isoform of glutamate decarboxylase that lacks an authentic calmodulin-binding domain at the c-terminus. | we have isolated full-length cdnas for two distinct isoforms of glutamate decarboxylase (gad), designated osgad1 and osgad2 from a rice shoot cdna library. open reading frames found in osgad1 and osgad2 cdnas encode putative proteins of 501 (56.7 kda) and 500 amino acids (55.6 kda), respectively. they show 69% identity to each other and 67-78% identity to dicotyledonous counterpart sequences determined so far. comparative analysis of relevant genomic clones obtained from the rice genomic library ... | 2001 | 11779628 |
| the dual effect of antifreeze protein on cryopreservation of rice (oryza sativa l.) embryogenic suspension cells. | the effects of fish antifreeze protein afp-i on cryopreservation of rice suspension cells by three different protocols were investigated. during the two-step method, afp-i at 0.01 mg/ml significantly lowered the viability of both precultured and non-precultured cells. during the vitrification method, afp-i at 0.2 mg/ml improved the viability of suboptimally thawed cells; however, much higher doses of this protein (10mg/ml) attenuated the cell viability. during rapid freezing of rice cells in the ... | 2015 | 11788857 |
| pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice (oryza sativa l.) embryogenic suspension cells. | rice embryogenic suspension cells were successfully cryopreserved by a pregrowth-desiccation procedure. cells were precultured in liquid aa medium containing 0.175 mol/l sucrose for 3 d and then in liquid aa medium containing 0.4 mol/l sorbitol for 1 d. after air-drying for about 20 h to a water content of 10%, the cells were placed into cryotubes and quenched into liquid nitrogen. using this pregrowth-desiccation procedure, a survival rate of 96+/-6% (ttc reduction assay) or 100% (cell clump re ... | 2015 | 11788862 |
| effect of erythrogen on post-thaw recovery of cryopreserved cell suspensions of indica rice (oryza sativa l.). | this study shows that adding haemoglobin solution (erythrogen) to post-thaw medium of indica rice (oryza sativa l.) cells enhances survival following cryopreservation. haemoglobin (1:50 - 1:200 v:v) had a beneficial effect on post-thaw viability and subsequent cell growth. a key finding was that the successful recovery from cryopreservation of cell suspensions of the indica rice cvs. br26 and pajam, and their re-establishment in aa2 medium, reflected a requirement for such supplementation of the ... | 2015 | 11788878 |
| methane production and oxidation in an anoxic rice soil as influenced by inorganic redox species. | the effects of addition of inorganic redox substances (species of no3-, mn4+, fe3+, and so4(2-)) on methane production and oxidation in anoxic rice (oryza sativa l.) soil samples were examined. sulfate was the most inhibitory for methane production followed by fe3+, no3-, and mn4+, in that order. addition of rice straw at a rate of 1% (w/w) as a carbon source to increase the electron donor to the electron acceptor ratio did not completely alleviate the inhibitory effects of redox species on meth ... | 2016 | 11790032 |
| antioxidant enzymes and dpph-radical scavenging activity in chilled and heat-shocked rice (oryza sativa l.) seedlings radicles. | chilling whole rice seedlings at 5 degrees c significantly increased the time needed to recover linear growth and reduced the subsequent linear rate of radicle growth. subjecting nonchilled seedlings to a 45 degrees c heat shock for up to 20 min did not alter subsequent growth, whereas a 3 min heat shock was optimal in reducing growth inhibition caused by 2 days of chilling. the activity of five antioxidant enzymes [superoxide dismutase (ec 1.15.1.1), catalase (cat; ec 1.11.1.6), ascorbate perox ... | 2002 | 11804522 |
| diverse variation of reproductive barriers in three intraspecific rice crosses. | reproductive barriers are thought to play an important role in the processes of speciation and differentiation. asian rice cultivars, oryza sativa, can be classified into two main types, japonica and indica, on the basis of several characteristics. the fertility of japonica-indica hybrids differs from one cross to another. many genes involved in reproductive barriers (hybrid sterility, hybrid weakness, and gametophytic competition genes) have been reported in different japonica-indica crosses. t ... | 2002 | 11805066 |
| [physiological indices for evaluating iron-toxicity tolerance of rice (oryza sativa l.)]. | this paper studied the content malodialdehyde (mda) and the leakage rate of electrolyte in leaves of various rice varieties with different iron-toxicity tolerance and of echinochloa crusgalli under excessive fe2+ (160 mg.l-1) stress. the results showed that both the mda content and the leakage rate of electrolyte in leaves of plants were significantly and negatively correlated with the tolerance of test plants to iron toxicity. it was suggested that both mda content and leakage rate of electroly ... | 2001 | 11813424 |
| ectopic expression of osmads3, a rice ortholog of agamous, caused a homeotic transformation of lodicules to stamens in transgenic rice plants. | in order to clarify the evolutionary relationship of floral organs between grasses and dicots, we expressed osmads3, a rice (oryza sativa l.) agamous(ag) ortholog, in rice plants under the control of an actin1 promoter. as a consequence of the ectopic expression of the osmads3, lodicules were homeotically transformed into stamens. in total, the transformation of lodicules to staminoid organs was observed in 18 out of 26 independent transgenic lines. in contrast to the almost complete transformat ... | 2002 | 11828031 |
| [qtl analysis of sheath blight resistance in rice (oryza sativa l.)]. | in this study, an indica variety zhai ye qing 8 (zyq8) and a japonica variety jing xi 17(jx17) and their double haploid (dh) population were used. sheath blight resistance of the dh population and their parents was investigated using a syringe inoculation method. quantitative trait loci (qtl) analysis was undertaken based on a constructed molecular linkage map of this population in hangzhou and hainandao respectively. a total of four sheath blight resistant qtls (qsbr-2, qsbr-3, qsbr-7 and qsbr- ... | 2002 | 11838375 |
| [developmental genetic analysis for transparency of rice (oryza sativa l.) at different environments]. | the developmental behavior of gene expression for transparency of rice (tr) was analyzed from two-year experimental data at different filling stages by using both conditional and unconditional analysis methods for quantitative traits of seeds in cereal crops. it was revealed that the gene expression was most active for the performance of tr at the middle and late filling stages (8-21 d after flowering). the differences of expression at different environments for triploid endosperm nuclear genes, ... | 2002 | 11838376 |
| relationship between ultraviolet-b sensitivity and cyclobutane pyrimidine dimer photorepair in rice. | among indica rice cultivars (oryza sativa l. cvs.) that belong to the aus ecotype from the tropical bengal region, where the amount of ultraviolet-b (uv-b) radiation in the solar radiation is relatively great, marich-bati cultivar has exhibited resistance to uv-b radiation, while surjamkhi cultivar appeared to be less resistant. we have examined the susceptibility to cyclobutane pyrimidine dimer (cpd) induction by uv-b radiation and the ability to photorepair cpds using these two cultivars. uv-b ... | 2001 | 11840646 |
| functional regions of rice heat shock protein, oshsp16.9, required for conferring thermotolerance in escherichia coli. | rice (oryza sativa) class i low-molecular mass (lmm) heat shock protein (hsp), oshsp16.9, has been shown to be able to confer thermotolerance in escherichia coli. to define the regions for this intriguing property, deletion mutants of this hsp have been constructed and overexpressed in e. coli xl1-blue cells after isopropyl beta-d-thioglactopyranoside induction. the deletion of amino acid residues 30 through 36 (patsdnd) in the n-terminal domain or 73 through 78 (eegnvl) in the consensus ii doma ... | 2002 | 11842169 |
| characterization of plant proliferating cell nuclear antigen (pcna) and flap endonuclease-1 (fen-1), and their distribution in mitotic and meiotic cell cycles. | the biochemical and cell cycle-dependent properties of proliferating cell nuclear antigen (ospcna) and flap endonuclease-1 (osfen-1) were characterized from rice (oryza sativa). ospcna was physically associated with osfen-1 and increased the flap-endonuclease activity of osfen-1 by 2.5-fold. northern and western blotting analysis revealed that ospcna and osfen-1 were present in meristematic tissues such as cultured cells, shoot apical meristem and root apical meristem. no expression was detected ... | 2001 | 11851910 |
| traditional production, consumption and storage of kunu--a non alcoholic cereal beverage. | a survey of the production, consumption and storage of kunu was carried out. some of the information included consumption rate, processing techniques and equipment, producer's status and grains used. about 73% consume kunu daily, 26% occasionally; 1% know it is produced but may or may not be consuming it. millet (pennisetum typhoideum), sorghum (sorghum vulgare), maize (zea mays), rice (oryza sativa) and acha (digitalis exilis) were used in its production in decreasing order of preference. the g ... | 2002 | 11855622 |
| effects of signaling molecules, protein phosphatase inhibitors and blast pathogen (magnaporthe grisea) on the mrna level of a rice (oryza sativa l.) phospholipid hydroperoxide glutathione peroxidase (osphgpx) gene in seedling leaves. | with a specific focus on rice self-defense response(s), the effects of global signaling molecules, jasmonic acid (ja), salicylic acid (sa), abscisic acid (aba), and ethylene (using the ethylene generator, ethephon), and protein phosphatase (pp) inhibitors, cantharidin and endothall on expression of a rice phospholipid hydroperoxide glutathione peroxidase (osphgpx) gene in rice seedling leaves were investigated. we provide first evidence for a potent up-regulation of the osphgpx mrna accumulation ... | 2002 | 11867229 |
| why phenolic acids are unlikely primary allelochemicals in rice. | allelopathy in rice (oryza sativa, l.) effective against weeds has been found in about 3.5% of tested rice germplasm in both laboratory and field experimentation. however, the allelochemicals responsible for growth inhibition of rice-associated weeds have not yet been identified. in the literature, phenolic acids are often mentioned as putative allelochemicals. if phenolic acids commonly reach growth inhibitory concentrations in rice ecosystems, it must be expected that the degree of tolerance t ... | 2002 | 11868676 |
| genetic basis for co-adaptive gene complexes in rice (oryza sativa l.) landraces. | one hundred and twenty-two aflp markers were mapped using an ir64 x azucena rice doubled-haploid (dh) population. the distribution of these mapped markers was monitored across a set of 48 diverse landraces of rice. strong statistical associations were observed between 960 of the 7381 possible pairs of markers across the diverse material. these 960 strongly associated pairs of markers mapped to the same chromosomes in only 111 cases. the remaining 849 pairs were the result of association between ... | 2001 | 11869343 |
| progress in breeding low phytate crops. | populations that depend on grains and legumes as staple foods consume diets rich in phytic acid (myo-inositol-1,2,3,4,5,6-hexkisphosphate), the storage form of phosphorus in seeds. this compound binds tightly to important mineral nutrients such as iron and zinc, forming salts that are largely excreted. this phenomenon can contribute to mineral depletion and deficiency. as one approach to solving this and environmental problems associated with seed-derived dietary phytic acid, the u. s. departmen ... | 2002 | 11880580 |
| spatial distribution of the 26s proteasome in meristematic tissues and primordia of rice (oryza sativa l.). | the 26s proteasome is known to play central roles in the growth of many eukaryotes. however, little is known regarding its distribution in higher plants. here, we report the spatial distribution pattern of rpn3 (a regulatory pa700 subunit) and c2 (a subunit of the 20s proteasome) in rice ( oryza sativa l.) seedlings as determined by in situ hybridization. the transcripts were abundantly co-expressed in the apical and marginal meristems of shoots and roots. interestingly, these transcripts also a ... | 2002 | 11882938 |
| effect of phenol on protein and amino acid content of xanthomonas oryzae pv. oryzae. | leaf blight disease of rice (oryza sativa) is caused by the bacterium xanthomonas oryzae pv. oryzae. phenol (1 to 4 mm) induced changes in protein profiles of x. o. pv. oryzae and a stress protein with a molecular mass of 69,000 appeared. hplc analysis indicated occurrence of amino acids such as asparagine, alanine, methionine and cystine in phenol treated cells. proton nmr analysis also revealed variation on the presence of amino acids in the cells treated with phenol. | 2001 | 11883515 |
| gene-containing regions of wheat and the other grass genomes. | deletion line-based high-density physical maps revealed that the wheat (triticum aestivum) genome is partitioned into gene-rich and -poor compartments. available deletion lines have bracketed the gene-containing regions to about 10% of the genome. emerging sequence data suggest that these may further be partitioned into "mini" gene-rich and gene-poor regions. an average of about 10% of each gene-rich region seem to contain genes. sequence analyses in various species suggest that uneven distribut ... | 2002 | 11891237 |
| uptake kinetics of arsenic species in rice plants. | arsenic (as) finds its way into soils used for rice (oryza sativa) cultivation through polluted irrigation water, and through historic contamination with as-based pesticides. as is known to be present as a number of chemical species in such soils, so we wished to investigate how these species were accumulated by rice. as species found in soil solution from a greenhouse experiment where rice was irrigated with arsenate contaminated water were arsenite, arsenate, dimethylarsinic acid, and monometh ... | 2002 | 11891266 |
| mlo, a modulator of plant defense and cell death, is a novel calmodulin-binding protein. isolation and characterization of a rice mlo homologue. | transient influx of ca(2+) constitutes an early event in the signaling cascades that trigger plant defense responses. however, the downstream components of defense-associated ca(2+) signaling are largely unknown. because ca(2+) signals are mediated by ca(2+)-binding proteins, including calmodulin (cam), identification and characterization of cam-binding proteins elicited by pathogens should provide insights into the mechanism by which ca(2+) regulates defense responses. in this study, we isolate ... | 2002 | 11904292 |
| novel genes are enriched in normalized cdna libraries from drought-stressed seedlings of rice (oryza sativa l. subsp. indica cv. nagina 22). | we have utilized an efficient method to enrich cdna libraries for novel genes and genes responsive to drought stress in rice (oryza sativa l. subsp. indica). we separately constructed standard and normalized cdna libraries from leaf tissue of rice seedlings grown under controlled drought stress. sequencing from the 3' end was performed on 1000 clones from the normalized leaf cdna library and 200 clones from the standard leaf cdna library. for the first 200 clones, the clone redundancy in the non ... | 2002 | 11908663 |
| catalytic properties of rice alpha-oxygenase. a comparison with mammalian prostaglandin h synthases. | long-chain fatty acids can be metabolized to c(n)(-1) aldehydes by alpha-oxidation in plants. the reaction mechanism of the enzyme has not been elucidated. in this study, a complete nucleotide sequence of fatty acid alpha-oxygenase gene in rice plants (oryza sativa) was isolated. the deduced amino acid sequence showed some similarity with those of mammalian prostaglandin h synthases (pghss). the gene was expressed in escherichia coli and purified to apparently homogeneous state. it showed the hi ... | 2002 | 11909851 |
| genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice. | immunocytological studies in this laboratory have suggested that nadh-dependent glutamate synthase (nadh-gogat; ec 1.4.1.14) in developing organs of rice (oryza sativa l. cv. sasanishiki) is involved in the utilization of glutamine remobilized from senescing organs through the phloem. because most of the indica cultivars contained less nadh-gogat in their sink organs than japonica cultivars, over-expression of nadh-gogat gene from japonica rice was investigated using kasalath, an indica cultivar ... | 2002 | 11912234 |
| effects of the cryopreservation procedures on recovered rice cell populations. | cryogenic storage of plant cells allows the long-term maintenance of valuable genotypes. cryopreservation of calli and cell suspensions is often performed using cryoprotectants and slow cooling rates. rice calli (oryza sativa l.) were cryopreserved by this procedure as well as by direct immersion in liquid nitrogen without cryoprotection. subsequently, the characteristics of the recovered cells as well as the effects of putative cryoselection were investigated by microscopic observations and flo ... | 2015 | 11912503 |
| identification and characterization of two genes encoding the eukaryotic initiation factor 4a in rice. | a cdna encoding eukaryotic translation initiation factor 4a (eif4a) was isolated from a cdna library of rice (oryza sativa l.). based on this dna sequence, a 414-amino acid protein exhibiting 67, 64 and 59% homology to the mouse, schizosaccharomyces pombe and saccharomyces cerevisiae eif4a, respectively, was predicted. the deduced amino acid sequence contains the characteristic motifs shared by the dead box supergene family. another cdna of rice eif4a was reported previously. comparison of the c ... | 2001 | 11913775 |
| a novel dna polymerase homologous to escherichia coli dna polymerase i from a higher plant, rice (oryza sativa l.). | a novel dna polymerase, designated as ospoli-like, has been identified from the higher plant, rice (oryza sativa l. cv. nipponbare). the ospoli-like cdna was 3765 bp in length, and the open reading frame encoded a predicted product of 977 amino acid residues with a molecular weight of 100 kda. the ospoli-like gene has been mapped to chromosome 8 and contains 12 exons and 11 introns. the encoded protein showed a high degree of sequence and structural homology to escherichia coli pol i protein, bu ... | 2002 | 11917019 |
| arsenic accumulation and metabolism in rice (oryza sativa l.). | the use of arsenic (as) contaminated groundwater for irrigation of crops has resulted in elevated concentrations of arsenic in agricultural soils in bangladesh, west bengal (india), and elsewhere. paddy rice (oryza sativa l.) is the main agricultural crop grown in the arsenic-affected areas of bangladesh. there is, therefore, concern regarding accumulation of arsenic in rice grown those soils. a greenhouse study was conducted to examine the effects of arsenic-contaminated irrigation water on the ... | 2002 | 11918027 |
| mutations that cause amino acid substitutions at the invariant positions in homeodomain of osh3 knox protein suggest artificial selection during rice domestication. | knox homeodomain (hd) proteins encoded by knotted1-like homeobox genes (knox genes) are considered to work as important regulators for plant developmental and morphogenetic events. we found that osh3, one of the knox genes isolated from a cultivar of oryza sativa (nipponbare), encodes a novel hd, which has two amino acid substitutions at invariant positions. sequence analysis of osh3 from various domesticated and wild species of rice has revealed that these substitutions are distributed only in ... | 2001 | 11922107 |
| comparative study of the reaction mechanism of family 18 chitinases from plants and microbes. | hydrolytic mechanisms of family 18 chitinases from rice (oryza sativa l.) and bacillus circulans wl-12 were comparatively studied by a combination of hplc analysis of the reaction products and theoretical calculation of reaction time-courses. all of the enzymes tested produced beta-anomers from chitin hexasaccharide [(glcnac)(6)], indicating that they catalyze the hydrolysis through a retaining mechanism. the rice chitinases hydrolyzed predominantly the fourth and fifth glycosidic linkages from ... | 2002 | 11926993 |
| multinuclear magnetic resonance analysis of two humic acid fractions from lowland rice soils. | to determine the effects of intensive cropping of tropical lowland rice (oryza sativa l.) and the associated long-term soil submergence on chemical properties of soil organic matter, we used solid-state 13c and 15n and solution 31p nuclear magnetic resonance (nmr) spectroscopy to analyze the labile mobile humic acid (mha) and the more recalcitrant calcium humate (caha) fractions extracted from a series of soils supporting several long-term field experiments in the philippines. the soils varied m ... | 2017 | 11931429 |
| a draft sequence of the rice genome (oryza sativa l. ssp. indica). | we have produced a draft sequence of the rice genome for the most widely cultivated subspecies in china, oryza sativa l. ssp. indica, by whole-genome shotgun sequencing. the genome was 466 megabases in size, with an estimated 46,022 to 55,615 genes. functional coverage in the assembled sequences was 92.0%. about 42.2% of the genome was in exact 20-nucleotide oligomer repeats, and most of the transposons were in the intergenic regions between genes. although 80.6% of predicted arabidopsis thalian ... | 2002 | 11935017 |
| a draft sequence of the rice genome (oryza sativa l. ssp. japonica). | the genome of the japonica subspecies of rice, an important cereal and model monocot, was sequenced and assembled by whole-genome shotgun sequencing. the assembled sequence covers 93% of the 420-megabase genome. gene predictions on the assembled sequence suggest that the genome contains 32,000 to 50,000 genes. homologs of 98% of the known maize, wheat, and barley proteins are found in rice. synteny and gene homology between rice and the other cereal genomes are extensive, whereas synteny with ar ... | 0 | 11935018 |
| a dual effect on protein synthesis and degradation modulates the tubulin level in rice cells treated with oryzalin. | the effect of the anti-microtubular drug oryzalin on growth and morphology of cultured rice (oryza sativa l., cv. roncarolo) cells was evaluated with specific reference to mechanisms that control intracellular tubulin levels. the addition of oryzalin caused a great reduction in the level of both alpha- and beta-tubulin polypeptides, as detected by western blot analysis. however, no appreciable decrease was observed in the population of total or isotype-specific alpha- and beta-tubulin mrnas. onl ... | 2002 | 11941459 |
| the rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfide isomerase. | rice (oryza sativa) accumulates prolamins and glutelins as storage proteins. the latter storage protein is synthesized on the endoplasmic reticulum (er) as a 57-kd proglutelin precursor, which is then processed into acidic and basic subunits in the protein storage vacuole. three esp2 mutants, cm1787, em44, and em747, contain larger amounts of the 57-kd polypeptide and corresponding lower levels of acidic and basic glutelin subunits than normal. electron microscopic observation revealed that esp2 ... | 2002 | 11950970 |
| the alpha-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium. | we recently isolated two genes (osga3ox1 and osga3ox2) from rice (oryza sativa) encoding 3beta-hydroxylase, which catalyzes the final step of active gibberellin (ga) biosynthesis (h. itoh, m. ueguchi-tanaka, n. sentoku, h. kitano, m. matsuoka, m. kobayashi [2001] proc natl acad sci usa 98: 8909-8914). using these cloned cdnas, we analyzed the temporal and spatial expression patterns of the 3beta-hydroxylase genes and also an alpha-amylase gene (ramy1a) during rice seed germination to investigate ... | 2002 | 11950975 |
| pyruvate,orthophosphate dikinase in leaves and chloroplasts of c(3) plants undergoes light-/dark-induced reversible phosphorylation. | pyruvate,orthophosphate (pi) dikinase (ppdk) is best recognized as a chloroplastic c(4) cycle enzyme. as one of the key regulatory foci for controlling flux through this photosynthetic pathway, it is strictly and reversibly regulated by light. this light/dark modulation is mediated by reversible phosphorylation of a conserved threonine residue in the active-site domain by the ppdk regulatory protein (rp), a bifunctional protein kinase/phosphatase. ppdk is also present in c(3) plants, although it ... | 2002 | 11950985 |
| expression of oryza sativa map kinase gene is developmentally regulated and stress-responsive. | mitogen-activated protein kinase (mapk) pathways are modules involved in the transduction of extracellular signals to intracellular targets in all eukaryotes. in plants, there is evidence for mapks playing a role in the signalling of abiotic stresses, pathogens, plant hormones, and cell cycle cues. the large number and divergence of plant mapks indicates that this ancient mechanism of signal transduction is extensively used in plants. however, there have been no reports of classical mapk module ... | 2002 | 11975731 |
| comparison of the structure of the extrinsic 33 kda protein from different organisms. | the psbo gene encoding the extrinsic 33 kda protein of oxygen-evolving photosystem ii (psii) complex was cloned and sequenced from a red alga, cyanidium caldarium. the gene encodes a polypeptide of 333 residues, of which the first 76 residues served as transit peptides for transfer across the chloroplast envelope and thylakoid membrane. the mature protein consists of 257 amino acids with a calculated molecular mass of 28,290 da. the sequence homology of the mature 33 kda protein was 42.9-50.8% b ... | 2002 | 11978871 |
| cell wall invertase in developing rice caryopsis: molecular cloning of oscin1 and analysis of its expression in relation to its role in grain filling. | to establish the significance of cell wall invertase in grain filling of rice (oryza sativa l.), we cloned a cdna for a cell wall invertase from developing grains of rice. the cdna, designated oscin1, contains an open reading frame of 1731 bp encoding a polypeptide of 577 amino acid residues. the deduced amino acid sequence showed typical features of the cell wall invertases, including a beta-fructosidase motif and a cysteine catalytic site, and shared 78.6 and 73.7% identity with maize cell wal ... | 2002 | 11978873 |
| expression of asparagine synthetase in rice (oryza sativa) roots in response to nitrogen. | the expression of asparagine synthetase (as; ec 6.3.5.4) in response to externally supplied nitrogen was investigated with respect to enzyme activity and protein levels as detected immunologically in rice (oryza sativa) seedlings. the asparagine content was very low in leaves and roots of nitrogen-starved rice plants but increased significantly after the supply of 1 mm nh4+ to the nutrient solution. while neither as activity nor as protein could be detected in leaves and roots prior to the suppl ... | 2002 | 11982933 |
| [detection and analysis of qtl for resistance to the brown planthopper, nilaparvata lugens (stål), in rice (oryza sativa l.), using backcross inbred lines]. | a mapping population of 98 bc1f9 lines (backcross inbred lines: bils), derived from a backcross of nipponbare (japonica)/kasalath (indica)//nipponbare by the single-seed descent methods, was used to detect quantitative trait loci (qtl) for resistance to brown planthopper (bph), nilaparvata lugens (stål). seedbox screening technique (sst) was applied to evaluate the reactions of two parents and 98 bils to bph at the seedling stage, and the entries were graded on ratio of dead seedlings. a total o ... | 2002 | 11985267 |
| characterisation of rice anther proteins expressed at the young microspore stage. | in combination with two-dimensional polyacrylamide gel electrophoresis (2-de) protein mapping and mass spectrometry analysis, the pattern of gene expression in specific tissues at a specific stage can be displayed and characterised. we used this approach for rice (oryza sativa l. cultivar doongara) to display and assign identity to proteins in the anthers at the young microspore stage. over 4000 anther proteins in the pi range of 4-11 and molecular mass range of 6-122 kda were reproducibly resol ... | 2001 | 11990509 |
| a fine physical map of the rice chromosome 4. | as part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome (bac)-based physical map of the oryza sativa japonica nipponbare chromosome 4 through an integration of 114 sequenced bac clones from a taxonomically related subspecies o. sativa indica guangluai 4 and 182 rflp and 407 expressed sequence tag (est) markers with the fingerprinted data of the nipponbare genome. the map consists of 11 contigs with a total length of 34.5 ... | 2002 | 11997348 |
| trends in comparative genetics and their potential impacts on wheat and barley research. | we review some general points about comparative mapping, the evolution of gene families and recent advances in the understanding of angiosperm phylogeny. these are considered in relation to studies of large-genome cereals, particularly barley (hordeum vulgare) and wheat (triticum aestivum), with reference to methods of gene isolation. the relative merits of direct map-based cloning in barley and wheat, utilization of the smaller genome of rice (oryza sativa) and gene homology methods that utiliz ... | 2006 | 11999846 |
| heat shocks increase the chilling tolerance of rice (oryza sativa) seedling radicles. | the growth of rice (oryza sativa l., cv. m202) seedling radicles, initially 10 +/- 1 mm long, was linear for the 96 h it took them to grow to 150 mm at 25 degrees c. exposure to 5 degrees c for 24 h reduced the rate of growth by about 50%, and longer exposures caused a progressive reduction in growth. initial radicle length significantly affected chilling sensitivity: with 2 days at 5 degrees c inhibiting growth at 25 degrees c by 23% for 1-mm radicles, 63% for 10-mm radicles, and 87% for 40-mm ... | 2002 | 12009992 |
| ethanol sensitivity of rice and oat coleoptiles. | the ability to avoid the ethanol-induced injury was evaluated in rice (oryza sativa l.) and oat (avena sativa l.) coleoptiles. the growth of the rice and oat coleoptiles was inhibited by ethanol exogenously applied at concentrations greater than 200 and 30 mm, respectively. at 300 mm ethanol, oat coleoptiles were brown and flaccid but rice coleoptiles did not show any visible symptoms of toxicity. the acetaldehyde level in rice and oat coleoptiles was increased by exogenously applied ethanol and ... | 2002 | 12010475 |
| solution structure of plant nonspecific lipid transfer protein-2 from rice (oryza sativa). | the three-dimensional structure of rice nonspecific lipid transfer protein (nsltp2) has been solved for the first time. the structure of nsltp2 was obtained using 813 distance constraints, 30 hydrogen bond constraints, and 19 dihedral angle constraints. fifteen of the 50 random simulated annealing structures satisfied all of the constraints and possessed good nonbonded contacts. the novel three-dimensional fold of rice nsltp2 contains a triangular hydrophobic cavity formed by three prominent hel ... | 2002 | 12011089 |
| tissue localization of a submergence-induced 1-aminocyclopropane-1-carboxylic acid synthase in rice. | at least two 1-aminocyclopropane-1-carboxylic acid synthase genes (acs) are implicated in the submergence response of rice (oryza sativa). previously, the os-acs5 gene has been shown to be induced during short- as well as long-term complete submergence of seedlings and to be controlled by a balance of gibberellin and abscisic acid in both lowland and deepwater rice. this study demonstrates that os-acs5 mrna is localized in specific tissues and cells both during normal development and in response ... | 2002 | 12011339 |
| glucosylation activity and complex formation of two classes of reversibly glycosylated polypeptides. | reversibly glycosylated polypeptides (rgps) have been implicated in polysaccharide biosynthesis. in plants, these proteins may function, for example, in cell wall synthesis and/or in synthesis of starch. we have isolated wheat (triticum aestivum) and rice (oryza sativa) rgp cdna clones to study the function of rgps. sequence comparisons showed the existence of two classes of rgp proteins, designated rgp1 and rgp2. glucosylation activity of rgp1 and rgp2 from wheat and rice was studied. after sep ... | 2002 | 12011358 |
| two uncoupling protein genes of rice (oryza sativa l.): molecular study reveals the defects in the pre-mrna processing for the heat-generating proteins of the subtropical cereal. | the recent finding of uncoupling proteins (ucps) in plants has demonstrated that plant mitochondria contain at least two distinct types of potential heat-generating protein, i.e. uncoupling proteins (ucps) and alternative oxidases. in this study, we isolated and characterized two rice genes encoding ucps with the aim of improving cold tolerance of rice through the modification of ucp expression. the two rice nuclear genes, osucp1 and osucp2, appeared to encode functional ucps, as far as the amin ... | 2002 | 12012245 |
| a high efficient approach used for bac-contig extension of oryza sativa with pcr screening the bac clone pools. | to extend 8 bac contigs, which were previously located in the 56.1-68 cm region of the chromosome 4 of the oryza sativa indica guangluai4, 14 pairs of primers were designed according to the terminal sequences of the existing seed bacs and were deliberately divided into 3 groups. with the 3 groups of primer mixtures, 233 pools of bac dna that represent 22 368 bac clones from o.sativa indica guangluai4 genomic library were screened. 65 positive clones corresponding to the 8 contigs were isolated a ... | 2002 | 12019452 |
| diversity of pseudomonas spp. isolated from rice rhizosphere populations grown along a salinity gradient. | along the coastline of tamil nadu, five sites were chosen to assess the diversity of pseudomonas populations isolated from rice (oryza sativa) cultivated along a salinity gradient. one of these sites was under organic farming while the other four were under inorganic farming. a total of 256 pseudomonas strains isolated from these five sites were analyzed using both phenotypic (substrate utilization patterns and antibiotic resistance assay) and genotypic (pcr-rflp of 16s rdna) characteristics. th ... | 2002 | 12023735 |
| genetic diversity in cultivars and landraces of oryza sativa subsp. indica as revealed by aflp markers. | genetic diversity among 49 indian accessions of rice (oryza sativa subsp. indica), including 29 landraces from jeypore, 12 modern cultivars, and 8 traditional cultivars from tamil nadu, was investigated using aflp markers. in total, nine primer combinations revealed 664 aflps, 408 of which were found to be polymorphic. the percentage of polymorphic aflps was approximately the same within the cultivars and landraces. similar results were obtained when genetic diversity values were estimated using ... | 2002 | 12033612 |
| organ-specific alternative transcripts of knox family class 2 homeobox genes of rice. | we identified three genes (hos58, hos59 and hos66) of rice (oryza sativa), which encode predicted proteins with the knox family class 2 homeodomain. these proteins contain three conserved domains, the knox domain, elk domain and homeodomain, from an n-terminus to a c-terminus. in addition to similarity of predicted amino acid sequences, these genes showed a similar exon/intron structure. cdna cloning and reverse transcription-polymerase chain reaction analyses of these genes indicated tissue-spe ... | 2002 | 12034492 |
| aox1c, a novel rice gene for alternative oxidase; comparison with rice aox1a and aox1b. | a novel gene for alternative oxidase (aox) was isolated from rice (oryza sativa l.) and characterized. the deduced amino acid sequence of the novel aox gene contains features that are conserved among other aoxs. this aox gene was designated aox1c based on a phylogenetic analysis of the aox genes. northern hybridization analyses revealed that aox1c and aox1a/aox1b transcripts accumulated differently in various rice organs and rice seedlings under low temperature conditions. aox1c mrna was mainly ... | 2002 | 12036102 |
| influence of different cultivars on populations of ammonia-oxidizing bacteria in the root environment of rice. | comparisons of the activities and diversities of ammonia-oxidizing bacteria (aob) in the root environment of different cultivars of rice (oryza sativa l.) indicated marked differences despite identical environmental conditions during growth. gross nitrification rates obtained by the 15n dilution technique were significantly higher in a modern variety, ir63087-1-17, than in two traditional varieties. phylogenetic analysis based on the ammonium monooxygenase gene (amoa) identified strains related ... | 2002 | 12039768 |
| isolation and characterization of a putative transducer of endoplasmic reticulum stress in oryza sativa. | following endoplasmic reticulum (er) stress that prevents correct folding or assembly of er proteins, at least three responses occur to maintain cell homeostasis: induction of chaperones, attenuation of protein synthesis, and enhancement of lipid synthesis. transducers that transmit er stress to the nucleus have already been identified in yeast and mammals. we report here isolation of a cdna, osire1, from rice encoding a putative homolog of ire1p, a yeast transducer of er stress. osire1 encodes ... | 2002 | 12040100 |
| purification and characterization of a novel 7-kda non-specific lipid transfer protein-2 from rice (oryza sativa). | a novel 7-kda non-specific lipid transfer protein-2 (nsltp2) has been isolated from rice (oryza sativa) seeds. in contrast to nsltp1s, few nsltp2s have been purified and characterized. complete amino acid sequence of rice nsltp2 was determined by n-terminal edman degradation of the intact protein as well as the peptide fragments resulted from trypsin digestions. rice nsltp2 consists of 69 amino acid residues with eight conserved cysteines forming four disulfide bonds. the secondary structure of ... | 2002 | 12056799 |
| a cdpk type protein kinase is involved in rice sps light modulation. | a protein kinase activity that can phosphorylate and inactivate rice (oryza sativa) sucrose-phosphate synthase (sps; udp-glucose: d-fructose-6-phosphate-2-glucosyl transferase, ec 2.4.1.14) was measured in extracts prepared from leaves exposed to light-dark transitions. enzyme activity present in extracts from dark leaves was about 5-fold higher than the activity in extracts from leaves that had been collected in the light. the protein kinase (named r-spsk) was purified about 100-fold from dark ... | 2002 | 12060234 |
| overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice. | in order to estimate whether cytosolic glutamine synthetase (gs1; ec 6.3.1.2) is partly coupled to the reaction of phenylalanine ammonia-lyase (pal; ec 4.3.1.5) in developing organs of rice (oryza sativa l.), we compared the expression pattern of transcripts and proteins for gs1 and pal in the tissue sections from leaf blades at various stages of development. in immature vascular bundles of unexpanded leaf blades, gs1 mrna was mainly detected in xylem parenchyma cells, mestome-sheath cells, and ... | 2001 | 12060286 |
| photochemical properties of the flavin mononucleotide-binding domains of the phototropins from arabidopsis, rice, and chlamydomonas reinhardtii. | phototropins (phot1 and phot2, formerly designated nph1 and npl1) are blue-light receptors that mediate phototropism, blue light-induced chloroplast relocation, and blue light-induced stomatal opening in arabidopsis. phototropins contain two light, oxygen, or voltage (lov) domains at their n termini (lov1 and lov2), each a binding site for the chromophore flavin mononucleotide (fmn). their c termini contain a serine/threonine protein kinase domain. here, we examine the kinetic properties of the ... | 2002 | 12068117 |
| isolation of novel rice (oryza sativa l.) multiple stress responsive map kinase gene, osmsrmk2, whose mrna accumulates rapidly in response to environmental cues. | in search for components of mapk (mitogen-activated protein kinase) cascades in rice (oryza sativa l. cv. nipponbare), we identified a single copy gene called osmsrmk2 from jasmonic acid (ja) treated rice seedling leaf cdna library. this gene has a conserved protein kinase domain, including a mapk family signature, and encodes a 369 amino acid polypeptide with a predicted molecular mass of 42995.43 and a pi of 5.48. osmsrmk2 did not show constitutive expression in leaves and was induced within 1 ... | 2002 | 12074577 |
| semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene. | the introduction of semidwarf rice (oryza sativa l.) led to record yield increases throughout asia in the 1960s. the major semidwarfing allele, sd-1, is still extensively used in modern rice cultivars. the phenotype of sd-1 is consistent with dwarfism that results from a deficiency in gibberellin (ga) plant growth hormones. we propose that the semidwarf (sd-1) phenotype is the result of a deficiency of active gas in the elongating stem arising from a defective 20-oxidase ga biosynthetic enzyme. ... | 2002 | 12077303 |
| inducibility by pathogen attack and developmental regulation of the rice ltp1 gene. | using a genomic clone encoding a rice lipid transfer protein, ltp1, we analysed the activity of the 5' region of the ltp1 gene in transgenic rice (oryza sativa l.) during plant development and under pathogen attack. the -1176/+13, -556/+13 and -284/+13 regions of the promoter were fused upstream from the uida reporter gene and nos 3' polyadenylation signal, resulting in the pdelta1176gus, pdelta556gus and pdelta284gus constructs which were transferred to rice by microprojectile bombardment. hist ... | 2002 | 12081375 |
| isolation and identification of a potent allelopathic substance in rice root exudates. | a search for growth inhibitors in rice root exudates was undertaken in order to clarify the allelopathic system in rice (oryza sativa l.). rice seedlings inhibited the growth of cress (lepidium sativum l.) and lettuce (lactuca sativa l.) seedlings when the cress and lettuce were grown with rice seedlings. the putative compound causing the inhibitory effect of rice seedlings was isolated from their culture solution, and the chemical structure of the inhibitor was determined by spectral data as mo ... | 2002 | 12081533 |
| stability of rice yellow mottle virus and cellular compartmentalization during the infection process in oryza sativa (l.). | rice yellow mottle virus (rymv) is icosahedral in morphology and known to swell in vitro, but the biological function of swollen particles remains unknown. anion-exchange chromatography was used to identify three markedly stable forms of rymv particles from infected plants: (1) an unstable swollen form lacking ca2+ and dependent upon basic ph; (2) a more stable transitional form lacking ca2+ but dependent upon acidic ph; and (3) a ph-independent, stable, compact form containing ca2+. particle st ... | 2002 | 12083840 |
| ostf1: a hd-gl2 family homeobox gene is developmentally regulated during early embryogenesis in rice. | in many eukaryotic organisms, homeobox genes are important regulators that specify the cell fate and body plan in early embryogenesis. in this study, a gene designated ostf1 (oryza sativa transcription factor 1) encoding a homeodomain protein in rice was isolated and characterized. the encoded ostf1, although sharing only approximately 51% sequence identity with other hd-gl2 members, contains four characteristic motifs (an n-terminal acidic region, a homeodomain, a truncated leucine zipper, and ... | 2002 | 12091716 |
| response of rice to al stress and identification of quantitative trait loci for al tolerance. | rice (oryza sativa l.) shows the highest tolerance to al toxicity among small-grain cereal crops, however, the mechanisms and genetics responsible for its high al tolerance are not yet well understood. we investigated the response of rice to al stress using the japonica variety koshihikari in comparison to the indica variety kasalath. koshihikari showed higher tolerance at various al concentrations than kasalath. the al content in root apexes was less in koshihikari than in kasalath, suggesting ... | 2002 | 12091719 |
| [obtaining stem borer-resistant homozygous transgenic lines of minghui 81 harboring novel cry1ac gene via particle bombardment]. | a modified cry1ac gene was generated by fusing with lys-asp-glu-lue (kdel), an endoplasmic reticulum retention signal at the 3'-ends, with signal peptide coding sequence of soybean kunitz trypsin inhibitor (skti) at the 5'-ends. vector containing the modified cry1ac gene coding region flanked by the corn ubiquitin 1 promoter and the nopaline synthase gene (nos) terminator with hygromycin phosphotransferase (hpt) gene as a plant selection marker was constructed. the modified cry1ac gene in which ... | 2002 | 12096630 |
| mitochondrial trna(trp)s imply a eubacterial origin. | three mitochondrial trna(trp)s genes from oryza sativa, homo sapiens and saccharomyces serevisea were constructed. in vitro transcripts of these mitochondrial trna(trp)s genes were able to be tryptophanylated by bacillus subtilis tryptophanyl-trna synthetase (trprs), but could not be catalyzed by trprs from rat liver. kinetic assay showed that b. subtilis trprs had half the binding capacity for mitochondrial trna(trp)s than for wild type trna(trp). while in the catalytic efficiency, the k(cat)/k ... | 2000 | 12098783 |
| cloning and characterization of rice (oryza sativa l) telomerase reverse transcriptase, which reveals complex splicing patterns. | plant chromosomes terminate in telomeres as in other eukaryotes. telomeres are vital to genome stability and their malfunctioning is lethal. one of the core components of the telomere complex is telomerase. the enzyme activity depends on rna (ter) and reverse transcriptase (tert) subunits. we describe here the isolation, sequencing and characterization of the telomerase reverse transcriptase catalytic subunit from the monocot plant oryza sativa l. (ostert). a single copy of this gene is present ... | 2002 | 12100484 |
| rice (oryza sativa l.) cultivars tolerant to high temperature at flowering: anther characteristics. | we examined the relationship between morphological characteristics of anthers and fertility in japonica rice cultivars subjected to high temperature (37.5(26 degrees c day/night) at flowering. percentage fertility was negatively correlated with the number of cell layers that separated the anther locule from the lacuna that formed between the septum and the stomium. the cell layers consisted of the remaining septum and degraded tapetum, and serve to keep the adjacent two locules closed. anther de ... | 2002 | 12102523 |
| expression of functional recombinant human lysozyme in transgenic rice cell culture. | using particle bombardment-mediated transformation, a codon-optimized synthetic gene for human lysozyme was introduced into the calli of rice (oryza sativa) cultivar taipei 309. the expression levels of recombinant human lysozyme in the transformed rice suspension cell culture approached approximately 4% of total soluble protein. recombinant human lysozyme was purified to greater than 95% homogeneity using a two-step chromatography process. amino acid sequencing verified that the n-terminus of t ... | 2002 | 12113455 |
| the barley mlo modulator of defense and cell death is responsive to biotic and abiotic stress stimuli. | lack of the barley (hordeum vulgare) seven-transmembrane domain mlo protein confers resistance against the fungal pathogen blumeria graminis f. sp. hordei (bgh). to broaden the basis for mlo structure/function studies, we sequenced additional mlo resistance alleles, two of which confer only partial resistance. wild-type mlo dampens the cell wall-restricted hydrogen peroxide burst at points of attempted fungal penetration of the epidermal cell wall, and in subtending mesophyll cells, it suppresse ... | 2002 | 12114562 |
| differential susceptibility of photosynthesis to light-chilling stress in rice (oryza sativa l.) depends on the capacity for photochemical dissipation of light. | the primary target for light-chilling stress in chilling-sensitive cucumber leaves is the chloroplast cu,zn-superoxide dismutase, followed by subsequent inactivation of the photosystem (ps) i by reactive oxygen species (ros). to test this hypothesis, two rice cultivars that were different in their ecological origins (a chilling-resistant stejaree 45 and a chilling-sensitive milyang 23) were evaluated with respect to photosynthetic properties, the ros scavenging system, and expression of genes th ... | 2002 | 12132582 |
| octadecanoid signaling component "burst" in rice (oryza sativa l.) seedling leaves upon wounding by cut and treatment with fungal elicitor chitosan. | octadecanoid pathway components, 12-oxo-phytodieonic acid (opda) and jasmonic acid (ja), are key biologically active regulators of plant self-defense response(s). however, to date these compounds have been studied mostly in dicots, and used large (1-10 g fresh weight, fw) samples for quantification, even when examined in mature rice plants, which is a drawback considering their rapid responsiveness to stress. focusing on rice--a monocot cereal crop research model--this work describes an efficien ... | 2002 | 12135598 |
| dasheng: a recently amplified nonautonomous long terminal repeat element that is a major component of pericentromeric regions in rice. | a new and unusual family of ltr elements, dasheng, has been discovered in the genome of oryza sativa following database searches of approximately 100 mb of rice genomic sequence and 78 mb of bac-end sequence information. with all of the cis-elements but none of the coding domains normally associated with retrotransposons (e.g., gag, pol), dasheng is a novel nonautonomous ltr element with high copy number. over half of the approximately 1000 dasheng elements in the rice genome are full length (5. ... | 2002 | 12136031 |
| a novel gene organization: intronic snorna gene clusters from oryza sativa. | based on the analysis of structural features and conserved elements, 27 novel snorna genes have been identified from rice. all of them belong to the c/d box-containing snorna family except for one that belongs to the h/aca box type. the newly found genes fall into six clusters that comprise at least three snorna genes, and in one case as many as nine genes. interestingly, four of the six clusters are located within the largest intron of a protein coding gene. the majority of intronic snorna gene ... | 2002 | 12136108 |
| crystallization and preliminary x-ray crystallographic studies of rice alpha-galactosidase. | alpha-galactosidases catalyze the hydrolysis of galactooligosaccharides and galactopolysaccharides to alpha-galactose residues and are widely distributed in microorganisms, plants and animals. alpha-galactosidase from rice (oryza sativa l. ssp. japonica) was crystallized by the hanging-drop vapour-diffusion method. the crystals belong to space group p2(1)2(1)2(1), with unit-cell parameters a = 63.1, b = 71.3, c = 85.6 a, and diffract beyond 1.9 a resolution. | 2002 | 12136160 |
| phytochrome mediates the external light signal to repress ft orthologs in photoperiodic flowering of rice. | phytochromes confer the photoperiodic control of flowering in rice (oryza sativa), a short-day plant. to better understand the molecular mechanisms of day-length recognition, we examined the interaction between phytochrome signals and circadian clocks in photoperiodic-flowering mutants of rice. monitoring behaviors of circadian clocks revealed that phase setting of circadian clocks is not affected either under short-day (sd) or under long-day (ld) conditions in a phytochrome-deficient mutant tha ... | 2002 | 12154129 |
| modulation of proton pumping across proteoliposome membranes reconstituted with tonoplast h(+)-atpase from cultured rice (oryza sativa l. var. boro) cells by acyl steryl glucoside and steryl glucoside. | tonoplast h(+)-atpase purified from cultured rice cells (oryza sativa l. var. boro) was reconstituted into asolectin liposomes containing steryl glucoside (sg) or acyl steryl glucoside (asg), and the effects of sg and asg on proton pumping, atp-hydrolysis activity and proton permeability of the proteoliposome membranes were investigated. in the proteoliposomes containing 10 mol% sg, proton pumping and atp-hydrolysis activity were increased to around 140% of those in sg-free proteoliposomes. in t ... | 2002 | 12154145 |
| molecular and biochemical characterization of a calcium/calmodulin-binding protein kinase from rice. | a ca2+/calmodulin (cam)-binding protein kinase from rice ( oryza sativa ), oscbk, has been characterized that lacks ca2+-binding ef hands and has ca2+/cam-independent autophosphorylation and substrate-phosphorylation activity. oscbk has all 11 subdomains of a kinase catalytic domain and a putative cam-binding domain, and shares high identity with ca2+-dependent-protein-kinase ('cdpk')-related protein kinases in plants. oscbk bound cam in a ca2+-dependent manner as previously reported for ca2+/ca ... | 2002 | 12160464 |
| molecular cloning and characterization of a novel beta-1,3-glucanase gene from rice. | beta-1,3-glucanases are referred to as pathogenesis-related proteins and they are also involved in several developmental processes. we isolated a cdna for beta-1,3-glucanase from rice anther and named it oryza sativa glucanase 1 (osg1). phylogenetic analysis showed that osg1 belonged to monocotyledonous endo-beta-1,3-glucanase subgroup a. rt-pcr analysis revealed that osg1 transcripts were present in leaves, roots, and anthers. | 2002 | 12162569 |
| proteomic survey of metabolic pathways in rice. | a systematic proteomic analysis of rice (oryza sativa) leaf, root, and seed tissue using two independent technologies, two-dimensional gel electrophoresis followed by tandem mass spectrometry and multidimensional protein identification technology, allowed the detection and identification of 2,528 unique proteins, which represents the most comprehensive proteome exploration to date. a comparative display of the expression patterns indicated that enzymes involved in central metabolic pathways are ... | 2002 | 12163647 |
| quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice (oryza sativa). | blast is an economically important disease of rice. to map genes controlling blast resistance, recombinant inbred lines (ril) were developed from khao dawk mali 105, an aromatic, blast-susceptible cultivar and the blast resistance donor, ct 9993-5-10-m (ct). a linkage map encompassing 2112 cm was constructed from 141 rils using 90 restriction fragment length polymorphisms (rflps) and 31 simple sequence repeats (ssr). virulent isolates of blast fungus were identified by screening differential hos ... | 2002 | 12168952 |
| abscisic acid and cytokinins in the root exudates and leaves and their relationship to senescence and remobilization of carbon reserves in rice subjected to water stress during grain filling. | the possible regulation of senescence-initiated remobilization of carbon reserves in rice (oryza sativa l.) by abscisic acid (aba) and cytokinins was studied using two rice cultivars with high lodging resistance and slow remobilization. the plants were grown in pots and either well-watered (ww, soil water potential = 0 mpa) or water-stressed (ws, soil water potential = -0.05 mpa) from 9 days after anthesis until they reached maturity. leaf water potentials of both cultivars markedly decreased at ... | 2002 | 12172848 |
| the novel rice (oryza sativa l.) gene ossbf1 encodes a putative member of the na+/bile acid symporter family. | pcr-based differential screening was used to identify ethylene-induced genes in deep-water rice (oryza sativa l.). one of the isolated cdnas represented a novel protein, ossbf1, with high homology to mammalian na+/bile acid transporters and to sodium-dependent transporters from bacteria. one highly homologous protein and three less conserved homologues were identified in arabidopsis thaliana indicating that sbf proteins exist in monocot and dicot plant species. expression of ossbf1 in deep-water ... | 2002 | 12177140 |
| high-level and ubiquitous expression of the rice cytochrome c gene oscc1 and its promoter activity in transgenic plants provides a useful promoter for transgenesis of monocots. | expression patterns of a rice (oryza sativa) cytochrome c gene oscc1 and its promoter activity were characterized in transgenic rice plants. oscc1 transcripts accumulate in most cell types, but to varying levels. large amounts of oscc1 transcripts are found in the roots, calli, and suspension cells, but relatively lower in mature leaves, demonstrating its higher levels of expression in non-photosynthetic tissues. unlike the human cytochrome c gene, which is responsive to camp, oscc1 expression i ... | 2002 | 12177461 |
| expression of a heterologous s-adenosylmethionine decarboxylase cdna in plants demonstrates that changes in s-adenosyl-l-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine. | we posed the question of whether steady-state levels of the higher polyamines spermidine and spermine in plants can be influenced by overexpression of a heterologous cdna involved in the later steps of the pathway, in the absence of any further manipulation of the two synthases that are also involved in their biosynthesis. transgenic rice (oryza sativa) plants engineered with the heterologous datura stramonium s-adenosylmethionine decarboxylase (samdc) cdna exhibited accumulation of the transgen ... | 2002 | 12177487 |
| barley cbf3 gene identification, expression pattern, and map location. | although cold and drought adaptation in cereals and other plants involve the induction of a large number of genes, inheritance studies in triticeae (wheat [triticum aestivum], barley [hordeum vulgare], and rye [secale cereale]) have revealed only a few major loci for frost or drought tolerance that are consistent across multiple genetic backgrounds and environments. one might imagine that these loci could encode highly conserved regulatory factors that have global effects on gene expression; the ... | 0 | 12177491 |
| identification of a soybean protein that interacts with gaga element dinucleotide repeat dna. | dinucleotide repeat dna with the pattern (ga)(n)/(tc)(n), so-called gaga elements, control gene expression in animals, and are recognized by a specific regulatory protein. here, a yeast one-hybrid screen was used to isolate soybean (glycine max) cdna encoding a gaga-binding protein (gbp) that binds to (ga)(n)/(ct)(n) dna. soybean gbp was dissimilar from the gaga factor of drosophila melanogaster. recombinant gbp protein did not bind to dinucleotide repeat sequences other than (ga)(n)/(ct)(n). gb ... | 2002 | 12177492 |
| two novel mitogen-activated protein signaling components, osmek1 and osmap1, are involved in a moderate low-temperature signaling pathway in rice. | rice (oryza sativa) anther development is easily damaged by moderately low temperatures above 12 degrees c. subtractive screening of cdna that accumulated in 12 degrees c-treated anthers identified a cdna clone, osmek1, encoding a protein with features characteristic of a mitogen-activated protein (map) kinase kinase. the putative osmek1 protein shows 92% identity to the maize (zea mays) mek homolog, zmmek1. osmek1 transcript levels were induced in rice anthers by 12 degrees c treatment for 48 h ... | 2002 | 12177502 |
| [analysis of gene loci and epistasis for drought tolerance in seedling stage of rice (oryza sativa l.)]. | drought tolerance of rice is important because a considerable proportion of the world rice area is not irrigated and is prone to water deficit. in this study, an indica variety, zhai ye qing 8 (zyq8), and a japonica variety, jing xi 17 (jx17), and their double haploid (dh) population were used for genetic study of drought tolerance. water supply was stopped in seedling period for 15 days and then drought tolerance of the dh population and their parents were investigated. mapping quantitative tra ... | 2002 | 12182078 |
| [physical mapping of the 45s rdna and 5s rdna to rice prometaphase chromosome]. | rice (oryza sativa l.) is one of the most important cereal crops in the world. the chromosomes of rice are relatively small in size. with the help of fluorescence in situ hybridization (fish), several rice dnas have been localized on rice chromosomes. 45s rdna and 5s rdna are encoding sequences for ribosomal rna synthesis. for detecting the chromosomes related to 45s rdna and 5s rdna, the digoxigenin-dutp labeled probe dna was probed to the prometaphase chromosomes which were prepared from the r ... | 2002 | 12182079 |