Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| glycosylated ovine prolactin. | a modification of prolactin, in which the asparagine at position 31 carries a carbohydrate unit, was isolated from ovine pituitary glands. sequence and amino acid analyses identified the point of linkage of the carbohydrate. glucosamine was found in acid hydrolysates, an indication that the carbohydrate is attached through n-acetylglucosamine. the glycosylated prolactin binds to concanavalin a and lentil lectin and is eluted with methyl alpha-d-mannopyranoside. during gel electrophoresis in sodi ... | 1984 | 6582495 |
| characterization of stimulatory activity for human pluripotent stem cells (cfugemm). | a medium conditioned by leukocytes in the presence of phytohemagglutinin (pha-lcm) promotes the growth of human multilineage hemopoietic progenitors cfugemm which form mixed hemopoietic colonies in culture containing granulocytes, erythroblasts, megakaryocytes, macrophages and mononuclear cells with t-cell antigens. in addition pha-lcm supports the growth of erythroid bursts (bfue) and granulocytic colonies (cfuc). stimulatory activities of pha-lcm were characterized by gel filtration using seph ... | 1983 | 6601024 |
| modulation of the biologic activities of ige-binding factor. ii. physicochemical properties and cell sources of glycosylation-enhancing factor. | t lymphocytes of rats treated with bordetella pertussis vaccine (bp) formed a soluble factor that enhanced the glycosylation of ige-binding factors during their biosynthesis, and provided the latter factors with the biologic activity to potentiate the ige response. the present experiments demonstrated that pertussigen (leukocytosis-promoting factor) from bp induced normal rat spleen cells to form the glycosylation-enhancing factor. the same factor was obtained by incubation of normal spleen cell ... | 1983 | 6601139 |
| clathrin-coated vesicles purified from a human b lymphocyte cell line contain newly synthesized glycoproteins. | clathrin-coated vesicles were purified from the cultured human b lymphocyte cell line rpmi 1788. these preparations were shown to be substantially free of contaminating membranes by the following criteria: (a) polypeptide composition, as assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, (b) electron microscopy, (c) lack of binding of intact vesicles to concanavalin a-sepharose, (d) migration of coated vesicles as a single major peak when applied to a sephacryl s-1000 gel fil ... | 1983 | 6602804 |
| t lymphocyte-mediated cytolysis. iii. delineation of mechanisms whereby mitogenic and non-mitogenic lectins mediate lymphocyte-target interaction. | we have investigated the mechanism(s) by which mitogenic (concanavalin a [con a], phytohaemagglutinin [pha] and lens culinaris agglutinin [lca]) and non-mitogenic (soybean agglutinin, peanut agglutinin, wheat-germ agglutinin and pokeweed mitogen) lectins mediate, non-specifically, lectin-dependent lymphocytotoxicity (ldcc). we show that non-mitogenic lectins are ineffective mediators of ldcc, due to their inability to mediate effective binding of effector cytotoxic t cells (ec) and target cells ... | 1983 | 6603417 |
| lymphocyte recognition of lymph node high endothelium. v. isolation of adhesion molecules from lysates of rat lymphocytes. | lymphocytes migrate from blood into lymph nodes (ln) of rats specifically at segments of venules lined by high endothelium (hev). investigation of lymphocyte surface molecules mediating this interaction has been carried out using an in vitro assay in which lymphocytes adhere selectively to hev when overlaid onto sections of peripheral ln. using this assay we have previously isolated a thoracic duct lymph component termed high endothelial binding factor (hebf), which is detected by its ability to ... | 1983 | 6604096 |
| trypanosomal surface coat variant antigen causes polyclonal lymphocyte activation. | variant antigen, the primary component of the surface coat of the salivarian trypanosome, when injected i.v. into mice at dosages encountered during acute infections, produced some of the immunopathogenic phenomena associated with acute african trypanosomiasis. trypanosome-infected and variant antigen-treated mice had splenomegaly caused by proliferation of null cells, b and t lymphocytes, and macrophages. splenic b lymphocytes were nonspecifically activated to produce antibodies to sheep erythr ... | 1983 | 6604754 |
| the reactivities of bauhinia purpurea and lens culinaris lectins to mouse b lymphocytes and their subsets. | the use of lectins for the separation of murine spleen lymphocyte subsets was examined. bauhinia purpurea agglutinin was found to be effective for the enrichment of b cells by differential agglutination. when the differential agglutination technique using lectins was applied to the separation of b cell subsets, lens culinaris agglutinin was found to be able to fractionate b cells into high responding and low responding subsets for dextran sulfate. a different expression of cell surface igd was a ... | 1983 | 6605940 |
| cell surface expression of cytotoxic t lymphocyte-defined, akr/gross leukemia virus-associated tumor antigens by normal akr.h-2b splenic b cells. | we previously described a system in which h-2kb-restricted c57bl/6 (b6) cytotoxic t lymphocytes (ctl) could be raised that were specific for tumors, such as the thymic lymphoma akr.h-2b sl1, that were induced by endogenous akr/gross murine leukemia virus and that expressed the gross cell surface antigen. in this study, certain normal lymphoid cells from akr.h-2b mice were also found to express target antigens defined by such anti-akr/gross virus ctl. akr.h-2b spleen, but surprisingly not thymus, ... | 1983 | 6605998 |
| purification to electrophoretic homogeneity of human alpha lymphotoxin from a cloned continuous lymphoblastoid cell line ir 3.4. | the 70-90,000 molecular weight (mw) alpha (alpha) component of the human lymphotoxin (lt) system has been purified to electrophoretic homogeneity. the alpha lt containing supernatants were obtained from a phorbol myristate (pma) stimulated cloned continuous human b lymphoblastoid cell line ir 3.4. supernatants were subjected to a biochemical separation scheme that consisted of chromatography on control pore glass beads, deae ion-exchange chromatography, lentil-lectin affinity chromatography, and ... | 1983 | 6606757 |
| human lymphotoxin. production by a lymphoblastoid cell line, purification, and initial characterization. | human lymphotoxin was purified to homogeneity from a serum-free tissue culture supernatant of a lymphoblastoid 1788 cell line. the purification scheme consisted of deae-cellulose chromatography, preparative isoelectric focusing, lentil lectin-sepharose chromatography, and preparative polyacrylamide gel electrophoresis. the purified glycoprotein was homogeneous by the criteria of high pressure liquid chromatography and polyacrylamide gel electrophoresis run under both nondenaturing and denaturing ... | 1984 | 6608523 |
| flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines. | the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ... | 1984 | 6609053 |
| isolation of serologically active hla-a, b, c and dr antigens as monitored by a fixed cell radioimmunoassay. | procedures for isolation of antigenically active preparations of human hla antigens were monitored using a sensitive and quantitative radioimmunoassay for hla-a, b, c or hla-dr antigens. these assays involve binding of radiolabeled monoclonal antibodies to appropriate target cells and inhibition of this binding by solubilized antigen preparations. fixation of the target cells with glutaraldehyde allows quantitation of inhibitors in high concentrations of nonionic detergents. using this assay, it ... | 1983 | 6609119 |
| ige-binding factors from mouse t lymphocytes. ii. strain differences in the nature of ige-binding factor. | normal spleen cells from high ige responder, bdf1 mice, formed ige-potentiating factor upon incubation with mouse ige, whereas those from low ige responder, sjl mice, formed ige-suppressive factor. the majority of the factors from bdf1 spleen cells had affinity for lentil lectin; those from sjl mice lacked affinity for the lectin. bdf1 spleen cells could be switched, however, to form ige-suppressive factor if the cells were incubated with ige in the presence of lipomodulin , a phospholipase inhi ... | 1984 | 6609987 |
| [quantification of lectin receptors on b, t, t-gamma and t-mu lymphocytes. i. concanavalin a, pisum sativum, lens culinaris and wheat-germ agglutinin]. | the immune system is formed of different lymphocyte subpopulations, each one having a defined role to defend the organism. their plasma membranes present differences in the glycoproteinic or/and glycolipidic composition, as detected with labelled 125i-lectins. b lymphocytes have a greater number of receptors for the pisum sativum, lens culinaris and wga lectins than t lymphocytes. on the other hand, t lymphocytes bind a greater number of concanavalin a molecules than b lymphocytes. wga lectin ap ... | 1984 | 6611567 |
| a survey of the nutritional and haemagglutination properties of legume seeds generally available in the uk. | eighty-five samples from fifteen different legume seed lines generally available in the uk were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. from these results the seeds were classified into four broad groups. group a seeds from most varieties of kidney (phaseolus vulgaris), runner (phaseolus coccineus) and tepary (phaseolus acutifolius) beans showed high reactivity with all cell type ... | 1983 | 6615758 |
| evaluation of the natural killer cell-interferon system in patients with mid-gut carcinoid tumours treated with leucocyte interferon. | nine patients with mid-gut carcinoid tumours received leucocyte interferon (ifn) i.m. daily for 90 days. six patients clearly ameliorated in symptoms typical of the carcinoid syndrome (flushing, diarrhoea, asthma) which correlated with reduced serum levels of tumour related polypeptides and urinary output of 5-hydroxyindole acetic acid (5-hiaa). before ifn treatment, peripheral blood mononuclear leucocytes (pbls) from carcinoid patients showed markedly deficient production of ph 2 labile ifn-alp ... | 1983 | 6616963 |
| allergens of schistosoma mansoni. i. comparison of the ige response in human and experimental infections towards characterized allergens from adult worm products. | the reaction of adult worm products of schistosoma mansoni with ige antibodies in infected human and fischer rat sera has been studied using the prausnitz-küstner test and the radioallergosorbent test. incubation of worms in water released most of the soluble material reacting with both rat and human sera in the radioallergosorbent test. sera from infected fischer rats recognized a fraction with pi 4.9-5.2 separated by preparative isoelectric focusing throughout the time course of infection, whe ... | 1983 | 6618684 |
| some properties of the plasma membrane of macrophages from germ-free rats. | the mobility of plasma membrane receptors for lectins concanavalin a (con a), phytohemagglutinin (pha), garden pea agglutinin (psa), lentil agglutinin (lca), peanut agglutinin (pna), soybean agglutinin (sba), wheat germ agglutinin (wga), and perch spawn agglutinin (pfa), the presence of binding sites for sheep erythrocytes, the presence of fc and complement receptors, as well as pinocytic and phagocytic activities were investigated in normal peritoneal macrophages from conventionally reared (cv) ... | 1983 | 6620259 |
| tumor stasis factor (tsf): a possible mechanism for the regulation of tumor cell proliferation. | a new class of factors that regulates tumor cell division in vitro can be isolated from fresh and cultured tumor cells by 3 m kcl extraction. tumor stasis factors (tsf) inhibiting cultured tumor cell proliferation were extracted from 8 of 11 fresh human tumors and 2 cultured tumor cell lines. tsf inhibited [3h]tdr incorporation by allogeneic and autologous cultured tumor cells in a dose-dependent manner. extracts of normal human tissues and benign tumors did not demonstrate inhibition with the e ... | 1983 | 6621026 |
| hu ly-m3--a human leukocyte antigen. | a monoclonal antibody, 5-4.8, was produced against human peripheral blood lymphocytes and it appears to be leukocyte-specific in that it reacts with a common determinant (called hu ly-m3) present on the peripheral blood t, b and null lymphocytes of 40 individuals. the antibody also reacts with thymocytes, spleen cells, and bone marrow cells (30%) and weakly with granulocytes and platelets--but not with heart, liver, or kidney. affinity to lentil-lectin and molecular weight analysis demonstrated ... | 1983 | 6623618 |
| the glycoprotein nature of solubilized muscarinic acetylcholine receptors from bovine cerebral cortex. | muscarinic acetylcholine receptors were solubilized from bovine cerebral cortex with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate. the so-obtained receptors could be precipitated by wheat germ (73%), concanavalin a (55%), lens culinaris (36%) and ricinus communis (26%), but not by peanut, dolichus biflorus and ulex europaeus. on wheat germ- and concanavalin a-affinity chromatography, the solubilized muscarinic receptors were retained on both columns and subsequently eluted with n- ... | 1983 | 6626225 |
| subsets of epidermal langerhans cells as defined by lectin binding profiles. | in this study we characterize the cell surface glycoconjugate moieties of strain 2 guinea pig epidermal langerhans cells (lc) in single cell suspension by using a battery of 17 fluorescent lectins. all lc displayed binding sites for concanavalin a, succinylated concanavalin a, lens culinaris agglutinin, pisum sativum agglutinin, wheat germ agglutinin, succinylated wheat germ agglutinin, griffonia simplicifolia agglutinin i, ricinus communis agglutinin i, phaseolus vulgaris e agglutinin, and phas ... | 1983 | 6631049 |
| heterogeneity of acetylcholine receptors: different forms of receptor distinguished by alpha-bungarotoxin kinetics and by antibody binding properties. | the interactions of the nicotinic acetylcholine receptor (achr), extracted from denervated rat hindlimb muscle, with alpha-bungarotoxin, concanavalin-a (con-a) and immunoglobulins isolated from the sera of specific patients with myasthenia gravis (mg) have been studied. the association and dissociation of toxin to unfractionated receptors [hydroxylapatite (htp)-achr] were best described in terms of two classes of kinetically distinct toxin binding sites present in approximately equal amounts. in ... | 1983 | 6631716 |
| respiratory syncytial virus glycoproteins. | the proteins of respiratory syncytial (rs) virus were analyzed by sds-polyacrylamide gel electrophoresis. eight virion structural proteins with molecular weights of 180,000, 89,000, 48,000, 42,000, 34,000, 28,000, 25,000, and 21,000 were identified. these proteins were given tentative designations of l (180,000), g (89,000), f1 (48,000), np (42,000), p (34,000), m (28,000), vp25 (25,000), and f2 (21,000). the 89,000-, 48,000-, and 21,000-dalton polypeptides were glycosylated and could be purifie ... | 1983 | 6636537 |
| structural features of the nerve growth factor inducible large external glycoprotein of pc12 pheochromocytoma cells and brain. | we have examined the oligosaccharide structure of a major mr = 230,000 cell surface glycoprotein from rat pc12 pheochromocytoma cells, and of the immunochemically cross-reactive species present in brain. in response to nerve growth factor (ngf) the pc12 cells extend long processes and acquire other properties similar to those of differentiated sympathetic neurons. these morphological changes are accompanied by a 3- to 5-fold increase in the concentration and labeling of this cell surface glycopr ... | 1983 | 6644303 |
| tumor-associated changes in plasma samples revealed by two-dimensional macromolecular mapping and selective lectin binding. | two-dimensional electrophoretic analysis of plasma samples from el-4 lymphoma-bearing c57 black mice revealed five 75 kd protein species in contrast with the presence of only two comparable components of similar migration in plasma from control animals. in contrast, no comparable alterations were observed in a comparison of plasma samples from l1210 tumor-bearing dba mice and the corresponding plasma from animals immune-suppressed with antilymphocytic serum or in control plasma from dba control ... | 1983 | 6667450 |
| [fractionation of lymphocytes using affinity chromatography with 9 lectins]. | lymphocyte subclasses from normal peripheral blood have been fractionated by affinity chromatography with lectins. concanavalin a (con a), lens culinaris lectin (lc), pisum sativum lectin (ps), phaseolus vulgaris lectin (pha), dolichos biflours lectin (db), glicine max lectin (sba), ricinus communis lectin (rca ii), tetragonolobus purpureus lectin (tp) and triticum vulgaris lectin (wga), were coupled to sepharose 6mb, and lymphocytes labelled with 125i were eluted through the chromatographic col ... | 1983 | 6675094 |
| reconstitution of the d-glucose transporter of bovine thymocyte plasma membrane: partial purification of transport activity by chromatography on agarose lentil lectin and agarose ethanethiol. | the d-glucose transporter of bovine-thymocyte plasma membrane was partially purified using several procedures in sequence. dimethylmaleic anhydride extraction removed extrinsic membrane proteins (approximately 50% of the total membrane protein) after which sodium cholate solubilized 40% of the residual protein. reconstitution of solubilized proteins into phospholipid liposomes indicated a 2.5-fold increase in sugar transport specific activity relative to membrane solubilized without dimethylmale ... | 1983 | 6681948 |
| cell-surface changes during mitogenic stimulation of lymphocytes assessed by the binding of wheat-germ agglutinin and other plant lectins. | lymphocytes from murine lymph node, cultured in the presence of an optimally mitogenic dose of phytohaemagglutinin, were stained with fluoresceinated lectins and analysed by flow cytometry. a marked increase in the ability of lymphocytes to bind wheat-germ agglutinin was observed that is particularly pronounced for the blast cells, reaching a maximum at about 40 h, when they are 5.5-times brighter than cells at zero time. the corresponding intensification of the small cells is 2-fold. much small ... | 1983 | 6688188 |
| interaction of human erythrocyte acetylcholinesterase with lectins. | interaction of acetylcholinesterase (a che) of human erythrocyte membranes with wheat germ lectin, concanavalin a, lens culinaris lectin and phytohaemagglutinin is described. in solutions containing triton x 100, only wheat germ lectin showed significant binding with ache. sepharose-immobilized wheat germ lectin bound ache significantly more than concanavalin a. this interaction was used for partial purification of the enzyme. | 1983 | 6688478 |
| presence in mouse bone marrow of mediator-dependent cytotoxic effector cells against tumors. | the role of bone marrow cells as cytotoxic effector cells against tumor cells was examined in vitro. bone marrow cells from normal c3h/he mice could lyse murine mm46 tumor cells in the presence of wheat germ agglutinin or syngeneic antitumor antibody. bone marrow cells alone did not cause lysis. eleven other lectins (concanavalin a, phytohemagglutinin, pokeweek mitogen, lens culinaris hemagglutinin, ulex europaeus agglutinin, peanut agglutinin, bandeiraea simplicifolia agglutinin, bauhinia purpu ... | 1983 | 6689597 |
| carbohydrate chain analysis by lectin binding to electrophoretically separated glycoproteins from murine b16 melanoma sublines of various metastatic properties. | cellular glycoprotein carbohydrate chains of b16 melanoma sublines of various metastatic colonization capacities were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and direct lectin staining, combined with chemical modification of carbohydrate chains in situ. for these studies, we utilized b16 sublines selected for low (b16-f1) or high lung (b16-f10), high brain (b16-b15b), or high ovary (b16-o13) colonization properties, or high tissue invasiveness in ... | 1984 | 6692378 |
| lectin-induced modulation of the antibody response to type iii pneumococcal polysaccharide. | several lectins were tested for their capacity to alter the antibody response to type iii pneumococcal polysaccharide (sss-iii). the antibody response was enhanced by concanavalin a (con a), phytohemagglutinin (pha), as well as lectins from phytolacca americana (pa-2), pisum sativum (psa), and lens culinaris (lch), when these lectins were given 2 days after immunization with sss-iii; however, suppression was obtained when con a and pa-2 were given at the time of immunization. by contrast the lec ... | 1984 | 6692477 |
| structural studies on glycoprotein oligosaccharides of chromaffin granule membranes and dopamine beta-hydroxylase. | dopamine beta-hydroxylase present in the soluble matrix of bovine adrenal medullary chromaffin granules contains biantennary complex oligosaccharides and high-mannose oligosaccharides in a molar ratio of approximately 2:1. the high-mannose oligosaccharides contain an average of six mannose residues. the largest biantennary oligosaccharides (40% of the total) have two complete peripheral branches consisting of sialic acid-galactose-n-acetylglucosamine, but an equal proportion lack sialic acid on ... | 1984 | 6696440 |
| primary structure of n-glycosidically linked asialoglycans of secretory immunoglobulins a from human milk. | the asialoglycopeptides obtained from secretory immunoglobulins a from human milk have been separated by gel filtration and affinity chromatography on concanavalin a-sepharose and lens culinaris agglutinin-sepharose columns. their structures have been determined by sugar analysis, methylation studies including mass spectrometry and 500-mhz 1h-nmr spectroscopy. the glycans are of the biantennary n-acetyllactosamine type differing in their degree of extension by fucosyl-n-acetyllactosamine residue ... | 1984 | 6698017 |
| monoclonal antibodies to rat liver cell membrane glycoproteins. | monoclonal antibodies were raised against membrane glycoproteins prepared from rat liver using lentil-lectin affinity chromatography. monoclonal antibodies to rat hepatocytes with three different characteristics were obtained and designated as ham.1, ham.2 and ham.3. the binding characteristics of these three monoclonal antibodies were analysed by flow cytofluorography and indirect radioimmunoassay; ham.3 recognized a membrane antigen restricted mainly to hepatocytes. however, most rat hepatoma ... | 1984 | 6727876 |
| receptors for pea and lentil lectins on human lymphoid cells: demonstration of distinct lectin-defined cell subclasses. | lectins are useful probes for studying cell surface glycoconjugates. pea (pl) and lentil (ll) lectin each requires for binding a fucosyl- and two alpha-mannosyl residues in core regions of glycopeptides, but differences in outer chain carbohydrates may alter their relative binding affinities. we used binding studies with [125i]-pl and ll and flow cytometry with fluorescein-conjugated (fitc)-pl and -ll to study their interactions with peripheral lymphocytes. binding of both lectins to lymphocytes ... | 1984 | 6736137 |
| change in glycosylation of membrane glycoproteins after transfection of nih 3t3 with human tumor dna. | altered glycosylation of membrane glycoproteins was demonstrated in nih 3t3 cells transformed by transfection with dna from human neuroblastoma and bladder carcinoma cell lines. the oncogenes of these two cell lines have been identified as n-ras and c-h-ras-1, respectively. the fucose-labeled membrane glycopeptides of transfection-induced transformants had decreased binding to concanavalin a-sepharose when compared in dual-isotope experiments to those from nih 3t3 cells, whereas binding to lenti ... | 1984 | 6744291 |
| electroimmunochemical analysis of plasma membrane vesicles from saccharomyces cerevisiae. | plasma membrane vesicles of saccharomyces cerevisiae were extracted with 1% (w/v) triton x-100 and the solubilized proteins examined by crossed immunoelectrophoresis using rabbit antibodies against the vesicles. solubilization was shown to be nonselective and 23 immunoprecipitates were observed reproducibly. four glycoproteins were identified by interaction with concanavalin a and lentil lectin, either immobilized on agarose beads in an intermediate gel or incorporated in the free form in the fi ... | 1982 | 6749264 |
| the pathogenic antigen of heymann nephritis is a membrane glycoprotein of the renal proximal tubule brush border. | purified brush border fractions prepared from rat kidneys were solubilized in detergent, iodinated, and subjected to immunoprecipitation to identify the pathogenic antigen present in brush border membranes that is responsible for the production of heymann nephritis (hn). purified igg prepared from the sera of rabbits or rats immunized with a crude cortical preparation, known as fx1a, precipitated multiple peptides, whereas igg eluted from glomeruli of rats with active or passive hn specifically ... | 1982 | 6752952 |
| carbohydrate determinants involved in both the binding and action of insulin in rat adipocytes. | the insulin receptor apparent affinity was markedly decreased in fat cells treated with lectins specific either for d-galactose (ricinus communis agglutinin i, rcai), d-mannose (concanavalin a, con a, lens culinaris agglutinin, lca) or n-acetyl-d-glucosamine (wheat germ agglutinin, wga), as indicated by a rightward shift of the binding competition curves and almost lineared scatchard plots. limulus polyphemus agglutinin (lpa), specific for sialic acid, was ineffective. all lectins enhanced 2-deo ... | 1982 | 6759201 |
| biosynthesis of lymphocyte surface igd in the mouse. | the synthesis of igd was studied in mouse spleen cells by using [35s]methionine labeling followed by immunoprecipitation with monoclonal antibody and two-dimensional gel electrophoresis. after a 15-min pulse of [35s]methionine, a relatively basic form (igd1) of apparent m.w. 59,000 was precipitated. conversion into more acidic forms of m.w. 63 to 72,000 (igd2) took place during a chase period of several hours. the acidic form was identical in mobility to that of igd labeled by surface radioiodin ... | 1980 | 6768796 |
| characterization of a plasma membrane-associated plasminogen activator on thymocytes. | plasma membranes isolated from normal thymocytes of hamster and rats were found to exhibit neutral protease activity toward 125i-labeled casein. the plasma membrane-associated proteases were completely inhibited by the serine protease inhibitors, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride and p-nitrophenyl-p-guanidinobenzoate, partially inhibited by soybean trypsin inhibitor and antipain, but were only weakly inhibited by l-1-tosylamino-2-phenylethyl chloromethyl ketone. the plas ... | 1981 | 6793069 |
| further characterization of the insulin receptor glycosidic moiety in rat adipocytes. | simultaneous or sequential treatment of rat adipocytes with neuraminidase plus beta-galactosidase decreased insulin binding by 43%. no modification was observed with either enzyme individually. alpha-mannosidase enhanced insulin binding (38%), whereas beta-n-acetylglucosaminidase and alpha-l-fucosidase were ineffective. lectins that interact with galactose (ricinus communis i, rcai), mannose, lens culinaris agglutinin (lca), concanavalin a (con a) or n-acetylglucosamine (wheat-germ agglutinin, w ... | 1981 | 6793421 |
| developmental stage-specific changes in lectin binding to mouse cerebellar cells in vitro. | eleven fluorescein isothiocyanate-conjugated (fitc) lectins, each with distinct carbohydrate-binding properties, were used to assess cell surface glycoconjugates of embryonic and early postnatal cerebellar cells in vitro. fluorescence staining of embryonic day 13 (e13) cerebellar cells with fitc ricinus communis agglutinin diminished markedly between 24 and 72 hr in vitro. no staining of postnatal day 0 (p0) or postnatal day 7 (p7) cells was observed with fitc ricinus communis agglutinin. a simi ... | 1981 | 6793697 |
| properties of a surface antigen expressed on activated human thymus-derived lymphocytes. | we have identified previously a quantitatively minor membrane protein (p28) with an apparent reduced m.w. of 28,000, which is biosynthetically labeled in activated human lymphocytes. rabbit antisera with activity directed against p28 (alpha-atc) were prepared and p28 was identified by immunoprecipitation in np-40 extracts of activated, extrinsically labeled lymphocytes. p28 was not expressed in appreciable amounts by unstimulated t cells, stimulated or unstimulated b cells, null cells, or adhere ... | 1982 | 6801123 |
| multiple molecular forms of rat brain enkephalinase. | rat brain enkephalinase has been partially purified by ion exchange chromatography, chromatofocusing, and affinity chromatography on immobilized lectins. ion exchange chromatography resolved two principle forms of enkephalinase designated a1 and a2. both enkephalinase a1 and a2 are bound to immobilized lentil lectin while chromatography on immobilized wheat germ lectin resolved each of the principle forms into two subforms, a1, 1, a1, 2, a2, 1, and a2, 2. all four enkephalinase forms have simila ... | 1982 | 6813615 |
| lectins as inducers of interferon-gamma production in human lymphocytes: lentil lectin is highly efficient. | several of many tested plant lectins induced interferon (ifn) production in cultures of human peripheral blood mononuclear leucocytes (pbl). the mannose-binding lectin obtained from lens culinaris (lcl) was a particularly efficient inducer of trypsin-sensitive antiviral activity, which qualified as ifn-gamma because it was 90-95% destroyed by ph 2 treatment but not neutralized by anti-ifn-gamma antibodies. however, such antibodies neutralized the residual 5-10% ph 2-resistant ifn, which therefor ... | 1982 | 6815785 |
| nonspecific and cooperative binding of lectins to microorganisms. | binding of lectins to microbial cell walls was investigated by fluorimetric titration and scatchard plot. data were correlated with agglutinability. concanavalin a and lectins of wheat germ, soybean, pea, lentil, and peanut were tested against escherichia coli. micrococcus luteus, lactobacillus plantarum, and bacillus subtilis. in cases where binding occurred, it was either nonspecific or positively cooperative. agglutination was observed only in those combinations of lectin and microorganism th ... | 1982 | 6817352 |
| flow cytometric analysis of lectin binding to human peripheral blood lymphocytes. | the binding of fluorescein-conjugated lentil lectin and concanavalin a to the surface membrane of human peripheral blood lymphocytes was studied by flow cytometry. the lymphocytes bound 3-fold more lentil lectin molecules compared with concanavalin a molecules and lentil lectin binding approached saturation at a much lower concentration than did that of concanavalin a. lentil lectin identified two groups of lymphocytes: a low-binding t-cell fraction and a high-binding b-cell-enriched fraction. c ... | 1982 | 6818949 |
| histochemical studies on lectin binding in reactive lymphoid tissues. | using the avidin-biotin-labeled peroxidase complex (abc) method, the staining reaction of a panel of 12 biotin-labeled lectins was studied in formalin-fixed, paraffin-embedded reactive lymph nodes and tonsils. varying degrees of lectin binding were observed in lymphoid cells and macrophage-histiocytes with concanavalin ensiformis (con a), lens culinaris (lca), phaseolus vulgaris (pha), pisum sativum (psa), ricinus communis (rca), and triticum vulgaris (wga) agglutinins, but no evidence of bindin ... | 1983 | 6827084 |
| functional reassembly of lymphocyte lentil lectin receptor glycoproteins into lipid bilayer vesicles. | plasma membrane vesicles purified from pig mesenteric lymph nodes were solubilized using the mild, readily-dialyzable detergent dodecyltrimethylammonium bromide, and lentil lectin receptor glycoproteins were isolated by affinity chromatography. the receptor fraction showed 12 major bands on sds-polyacrylamide gel electrophoresis representing 8-9% of the membrane protein. 5'-nucleotidase (ec 3.1.3.5) was very effectively solubilized by the detergent and was recovered in high yield in the receptor ... | 1983 | 6830799 |
| cell type-specific binding of ricinus lectin to murine cerebellar cell surfaces in vitro. | the binding of several plant lectins, concanavalin a (con a), lens culinaris a (lca), wheat germ agglutinin (wga), and ricinus communis agglutinin 120 (rca 120) to cell surfaces of developing mouse cerebellar cells was assayed by the use of fluorescein isothiocyanate (fitc)-conjugated compounds. freshly dissociated, live single-cell suspensions from 6-day-old mouse cerebellum contain 93% cona, 99% lca, 98% wga, and 59% rca 120-positive cells with ring fluorescence. of the rca 120-positive cells, ... | 1983 | 6831524 |
| effects of common radioiodination procedures on the binding of glycoproteins to immobilized lectins. | representative glycoproteins including fetuin, protein a, ovalbumin, alpha 1 acid glycoprotein, and the major glycoprotein of equine infectious anemia virus were labelled with 125i by the chloramine-t or bolton-hunter procedure and their binding to immobilized con a or lentil lectin compared to untreated samples of each glycoprotein. glycoprotein modification was no greater than one substituted residue per protein molecule. yet the radioiodinated glycoproteins typically displayed only 0-50% of t ... | 1983 | 6838504 |
| lymphocyte subpopulations in chronic lymphocytic leukemia detected by lectin binding and flow cytometry. | flow cytometry was used to compare binding of wheat germ agglutinin (wga) and lens culinaris lectin (lcl) to peripheral lymphocytes (pbl) from normal subjects and 26 patients with chronic lymphocytic leukemia (cll). as defined by the interactions of wga and lcl with cell surface receptors, normal unfractionated pbl and purified b-cells were heterogeneous, containing subclasses that bound to wga (wga+lcl-), lcl (wga-lcl+), both (wga+lcl+), or neither (wga-lcl-). by contrast, in 25 of 26 cll patie ... | 1983 | 6839300 |
| interactions of purified human ceruloplasmin with lathyrus odoratus, lens culinaris and canavalia ensiformis lectins. | human ceruloplasmin was isolated from normal serum by fractional polyethylene glycol precipitation and subsequent ion exchange chromatography. the molecular mass determined by ultracentrifugation was 132 kda and an optical density ratio (a610/a280) of 0.046 was observed in the preparation. immunoelectrophoresis, agarose-gel electrophoresis and n-terminal amino-acid sequence analyses showed that the ceruloplasmin preparation was highly purified, while dodecyl sulphate polyacrylamide-gel electroph ... | 1983 | 6840700 |
| ice nucleating activity of pseudomonas syringae and erwinia herbicola. | chemical and biological properties of the ice nucleating sites of pseudomonas syringae, strain c-9, and erwinia herbicola have been characterized. the ice nucleating activity (ina) for both bacteria was unchanged in buffers ranging from ph 5.0 to 9.2, suggesting that there were no essential groups for which a change in charge in this range was critical. the ina of both bacteria was also unaffected by the addition of metal chelating compounds. borate compounds and certain lectins markedly inhibit ... | 1983 | 6848483 |
| clonal myoblasts and myotubes show differences in lectin-binding patterns. | to determine changes in distribution or mobility of cell-surface glycoconjugates during myogenesis the binding of fluorescein-conjugated plant lectins to myoblasts and myotubes of the l6 rat skeletal muscle cell line has been studied. binding has been carried out at 4 degrees c on either live or glutaraldehyde-fixed cells. fluorescein conjugates of soybean agglutinin (fl-sba), wheat germ agglutinin (fl-wga), concanavalin a (fl-cona) and lens culinaris agglutinin (fl-lca) produced predominantly u ... | 1983 | 6852121 |
| crystallization and preliminary x-ray diffraction studies of the mitogenic lentil (lens culinaris) lectin. | 1983 | 6854638 | |
| induction of polymorphonuclear leukocyte-mediated cytolysis by wheat germ agglutinin and antitumor antibody. | the role of polymorphonuclear leukocytes (pmns) as effector cells in tumor lysis was investigated in vitro. pmns were obtained from the peritoneal cavity of c3h/he mice injected ip with 2 ml of 12% casein sodium. these pmns could lyse murine mm46 tumor cells in the presence of the plant lectin, wheat germ agglutinin (lectin-dependent pmn-mediated cytolysis). pmns alone did not cause cytolysis. pmns obtained 6 hr after casein injection were effective against tumor target cells but those obtained ... | 1983 | 6862146 |
| inter- and intramolecular disulfide bonding among lymphocyte plasma membrane proteins and glycoproteins. | the disulfide bonding characteristics of the pig lymph node plasma membrane (pm) proteins and glycoproteins have been examined by 1- and 2-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). reaction of the purified pm vesicles with n-ethyl maleimide (nem) prior to detergent solubilization was found to markedly reduce the extent of intermolecular disulfide bonding subsequently observed. thus the blocking of free sulfhydryl groups with nem prevented the detergent-ind ... | 1983 | 6865956 |
| identification of an oncofetal antigen (gp90) on murine b16 melanoma cells. | a xenoantiserum to murine b16 melanoma cells was developed by immunizing rabbits with cultured b16 melanoma cells. this antiserum could, after extensive absorption with normal c57bl/6 mouse tissues, react with syngeneic (b16) and allogeneic (hp) melanomas as well as with other murine neoplasms, including syngeneic 75s adenocarcinoma, allogeneic myeloma and leukemic t cell lines. the antiserum also cross-reacted with syngeneic fetal fibroblasts and with an allogeneic fetal fibroblast cell line (s ... | 1982 | 6889531 |
| lectin affinity chromatography of glycopeptides and oligosaccharides from normal and lectin-resistant chinese-hamster ovary cells. | the [3h]mannose-labelled glycopeptides from two lectin-resistant lines of chinese-hamster ovary cells were fractionated by chromatography on lentil lectin-sepharose and concanavalin a-agarose columns and subsequently analysed by gel filtration in comparison with the glycopeptides of the parental cell line. essentially all of the [3h]mannose-labelled asparaginyl-oligosaccharides from the 'single-mutant' cells selected for resistance to phytohaemagglutinin and the 'double-mutant' cells selected fo ... | 1982 | 6890813 |
| synergistic effects of lectins in the interaction of thrombin with human platelets. | several lectins have been studied for their effects on the interaction of thrombin with human platelets. wheat germ agglutinin, concanavalin a and ricinus communis lectin increased the number of high affinity sites for diisopropylphosphothrombin on washed platelets from 3000 to about 12 000 but the binding affinities were unchanged (kd approx 4 nm). two other lectins, lens culinaris and bandieria simplicifolia, were without effect. (2) using formalinized platelets to avoid possible complications ... | 1981 | 6894391 |
| lectin-binding patterns of small lymphocytes in bone marrow, thymus and spleen: demonstration of lymphocyte subsets by quantitative radioautography. | cells from mouse bone marrow, thymus and spleen were exposed to 125i-labeled concanavalin a (con a). lens culinaris lectin (lcl), soybean agglutinin (sba), helix pomatia agglutinin (hpa), phytohemagglutinin-p (pha-p), peanut agglutinin (pna), or wheat germ agglutinin (wga) in a range of concentrations and examined radioautographically. small lymphocytes in the three organs differed in the minimal concentration of each lectin which gave detectable surface labeling, while at optimal lectin concent ... | 1981 | 6895730 |
| binding of lectins to leukemic cell lines. | the binding of 12 different fluorescein-conjugated lectins to 10 all (acute lymphoblastic leukemia) cell lines and two cell lines derived from patients in blast crisis of myeloid leukemia was examined. the specificity of the membrane fluorescence was demonstrated by inhibition with various saccharides. three of the lectins bound to all cell lines, four bound to only some of the lines, and five were not bound. there was no correlation between the binding pattern and the immunological phenotype of ... | 1980 | 6928109 |
| surface glycoproteins of human sarcoma- and fibroblastic cells. | a comparison was made of the cell surface glycoproteins of four human cell lines, namely a giant tumor of bone cell line, an osteosarcoma line, a fibrosarcoma line and a human fibroblast line. the cells were labeled by lactoperoxidase catalyzed iodination and the glycoproteins extracted by 0.5% triton x-100 were bound to lentil-lectin and subsequently analyzed by sds gel electrophoresis. while the cell lines examined shared a series of common glycoproteins, it was found that the giant cell tumor ... | 1980 | 6942238 |
| analysis of murine ia antigen glycosylation by lectin affinity chromatography. i-ak alpha chain subspecies and beta chains are each glycosylated differently. | murine ia antigens consist of two glycosylated polypeptide chains, the alpha chain and the beta chain. we have used lectin affinity chromatography to confirm previous work in our laboratory that three distinct, differentially glycosylated i-ak alpha chains (alpha 1, alpha 2, and alpha 3) exist and to compare the carbohydrate of the alpha chain with that of the beta chain. glycopeptides derived from pronase digestion of [3h]mannose-labeled i-ak alpha 1, alpha 2, alpha 3, and beta chains were sequ ... | 1982 | 6956576 |
| detection, isolation and characterization of cell free hla a and b antigens from human amniotic fluid. | cell-free hla a and b antigens, paternal and maternal, have been detected in human amniotic fluids obtained from women at 16-18 weeks' gestation. fractionation of amniotic fluid samples on sephadex showed that hla a and b antigen activity was mainly in the 40,000 to 70,000 mol. wt. fractions. lentil lectin sepharose 4b affinity chromatography of 30,000 to 70,000 sephadex molecular weight fraction of amniotic fluids isolated proteins which gave 2 bands, m and n, on discontinuous polyacrylamide ge ... | 1982 | 6959379 |
| comparison between surface components from human b and chronic lymphatic leukaemia lymphocytes isolated by lentil lectin. | peripheral blood lymphocytes from patients with chronic lymphatic leukaemia (cll) were compared to normal purified b lymphocytes. lymphocytes were cultured in the presence of 3h-fucose, and glycoproteins isolated by lentil lectin, were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. most of the major components detected in normal b lymphocytes were also present in cll lymphocytes. although b and cll lymphocytes shared several components of similar molecular size, the latt ... | 1980 | 6965284 |
| ia requirement in t cell response to various mitogenic lectins. | various lectins, mainly t cell mitogens, were examined to determine the relationship between anti-ia antiserum-treated t cell responses and the sugar specificities of these lectins. anti-ia antiserum and complement treatment diminished nylon wook column-purified t cell responses to most lectins such as concanavalin a, lens culinaris hemagglutinin, pisum sativum agglutinin, witaria floribunda mitogen, limulus polyphemus agglutinin and pokeweed mitogen, and the diminished response to these lectins ... | 1980 | 6965926 |
| regulatory role of ige-binding factors from rat t lymphocytes. ii. glycoprotein nature and source of ige-potentiating factor. | previous experiments have shown that fc epsilon receptor-bearing (fc epsilon r(+)) t lymphocytes in mesenteric lymph nodes (mln) of rats infected with nippostrongylus brasiliensis (nb) release a soluble factor that selectively potentiates the ige response. the ige-potentiating factor has affinity for ige, and can be detected by the ability to inhibit rosette formation of fc epsilon r(+) cells with ige-coated erythrocytes. the factor was bound to ige-coated sepharose and was eluted from the beads ... | 1980 | 6967902 |
| regulatory role of ige-binding factors from rat t lymphocytes. iii. ige-specific suppressive factor with ige-binding activity. | incubation with rat ige of rat mesenteric lymph node cells obtained 8 days after infection with nippostrongylus brasiliensis (nb) resulted in the formation of soluble factors with affinity for ige, i.e., ige-binding factors(s). the factors were derived from t lymphocytes and were able to suppress an in vitro ige response without affecting the igg response. the minimum concentration of rat ige for the induction of factor formation was 0.3 to 1.0 microgram/ml. gel filtration of culture filtrates o ... | 1980 | 6967903 |
| regulatory role of ige-binding factors from rat t lymphocytes. iv. formation of ige-binding factors in rats treated with complete freund's adjuvant. | mesenteric lymph node (mln) cells from rats treated with complete freund's adjuvant (cfa) spontaneously released soluble factors, which have affinity for ige (ige-binding factors). the factors were purified by absorption of culture filtrates of the lymphocytes with ige-sepharose followed by elution at acid ph. purified ige-binding factors selectively suppressed ige-forming cell response of dnp-ovalbumin-primed mln cells to homologous antigen without affecting the igg2 response. fractionation of ... | 1980 | 6968774 |
| novel surface antigen expressed on dividing cells but absent from nondividing cells. | radioimmunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to study the distribution on human lymphoid cells of a previously undescribed surface antigen recognized by several heteroantisera. a glycoprotein with a 90,000 mol wt (under reducing conditions) was detected on all cell lines tested including t, b, null, and myeloid cell lines, although the amount of antigen present varied considerably. the antigen was absent from normal peripheral blood lymp ... | 1980 | 6968814 |
| regulatory role of ige-binding factors from rat t lymphocytes. v. formation of ige-potentiating factor by t lymphocytes from rats treated with bordetella pertussis vaccine. | an i.p. injection of bordetella pertussis vaccine (bp) into rats induced the formation of soluble factors that had affinity for ige (ige-binding factors). the factor was detected in the serum of bp-treated animals 5 to 7 days after the treatment. their circulating lymphocytes as well as spleen cells spontaneously released ige-binding factors in the serum of bp-treated rats and those released from their circulating lymphocytes had affinity for lentil lectin, and the ability to selectively potenti ... | 1981 | 6970222 |
| lymphocytes bearing fc receptors for ige. v. effect of tunicamycin on the formation of ige-potentiating factor and ige-suppressive factor by con a-activated lymphocytes. | attempts were made to induce the formation of soluble factors having affinity for ige (ige-binding factors) by activated t cells. normal rat mesenteric lymph node (mln) cells were cultured in 1 microgram/ml con a for 48 hr or in 10 microgram/ml con a for 72 hr, and con a-activated lymphocytes were incubated with rat ige. it was found that ige induced an increase in the proportion of fc epsilon r(+) cells in the con a-activated cells and formation of ige-binding factors. however, the nature of ig ... | 1981 | 6970224 |
| lymphocytes bearing fc receptors for ige. vi. suppressive effect of glucocorticoids on the expression of fc epsilon receptors and glycosylation of ige-binding factors. | incubation of rat lymphocytes with homologous ige induced an increase in fc epsilon r(+) lymphocytes and the formation of ige-binding factors. pretreatment of rat lymphocytes with 1 to 5 microm dexamethasone, however, prevented the ige-induced expression of fc epsilon r on both b and t lymphocytes. upon incubation with ige, t cells activated with 10 micrograms/ml con a produced ige-potentiating factors that had affinity for lentil lectin and selectively enhanced the ige response. pretreatment of ... | 1981 | 6972964 |
| analysis of human t-cell antigens by one- and two-dimensional polyacrylamide gel electrophoresis. | one- and two-dimensional polyacrylamide gel electrophoresis (page) was performed on immunoprecipitates formed between anti-human t-cell xeno-antiserum (ats) and cell-surface glycoproteins of human lymphocytes, that had been radioiodinated by lactoperoxidase and purified on a lentil lectin-coupled sepharose 4b column. in some experiments, the cells were 3h-labeled by periodate-tritiated borohydride. ats that was absorbed with b cells recognized a number of cell-surface antigens expressed preferen ... | 1981 | 6974300 |
| biochemical and immunologic heterogeneity of ia glycoproteins isolated from a chronic lymphocytic leukemia. | ia glycoproteins have been isolated from human chronic lymphocytic leukemic cells (cll) by lens culinaris chromatography and by filtration on aca-34 ultrogel. ia antigenic activity, measured by inhibition of the cellular radioimmunoassay, was separated by gel filtration into 2 fractions, peak i and peak ii. monoclonal antibodies, produced against peak ii glycoproteins, appear to recognize different antigenic determinants of ia molecules. monoclonal antibody 18a4 reacted with ia molecules of peak ... | 1982 | 6976373 |
| formation of rat ige-binding factors by rat-mouse t cell hybridomas. | mesenteric lymph node (mln) cells of rats infected with nippostrongylus brasiliensis (nb) were obtained 8 days after infection and were incubated overnight with rat ige for the formation of ige-binding factors. these cells were then fused with bw 5147 mouse t lymphoma cells. two hybrid clones (a4 and b6) obtained by the fusion formed ige-binding factors upon incubation with ige. it was found that 1-hr incubation with 3 micrograms/ml of rat ige was sufficient to induce hybridoma cells to form ige ... | 1982 | 6979575 |
| studies on the mechanism of lectin-dependent t cell-mediated cytolysis: use of lens culinaris hemagglutinin a to define the role of lectin. | 1982 | 6980578 | |
| characterization of a 75,000 mol. wt glycoprotein synthesized by guinea-pig t-lymphocytes: a possible homologue of lyt-1 antigen. | a xenoantiserum raised in rabbits by immunization with strain 13 guinea-pig antigen-activated t-lymphocytes was previously found to recognize a non-immunoglobulin, 75,000 mol. wt glycoprotein synthesized by guinea-pig t-cells. this protein, p75, has been further characterized to determine its biochemical properties and its expression by various cell types. p75 was found to be a single-chain protein which could be bound by the lectin lens culinaris hemagglutinin. it has an apparent mol. wt slight ... | 1982 | 6984489 |
| induction and separation of mouse helper t cells by lectins. | the capability of lens culinaris agglutinin (lca) to induce selectively the helper t cell activity affecting primary antibody response was demonstrated. in the presence of mouse spleen cells, activated with lca at a concentration of 12 . 5 microgram/ml, optimal augmentation of the humoral immune response to sheep erythrocytes (srbc) was observed. it was also demonstrated that limulus polyphemus agglutinin (lpa), which was shown to possess carbohydrate-binding specificity directed to sialic acid ... | 1980 | 6997194 |
| lectin receptors as markers for trypanosoma cruzi. developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. | trypanosoma cruzi at various stages of maturation and differentiation have been isolated by conventional cellular fractionation procedures and characterized by cell surface markers using 30 highly purified lectins encompassing all known sugar specificities. cell surface carbohydrates of the various t. cruzi stages were analyzed by agglutination and lectin-binding assays. specific receptors for wheat germ agglutinin (wga), helix pomatia, sophora japonica, and bandeiraea simplicifolia lectin ii we ... | 1980 | 7000967 |
| skin allograft survival in lentil lectin-treated mice and rats. | 1981 | 7022800 | |
| identification and purification of a mr 75,000 cell surface human melanoma-associated antigen. | surface macromolecules on cultured human melanoma cells wee radioiodinated by a lactoperoxidase method. macromolecules shed into culture medium were collected, concentrated, and fractionated on sepharose 6b and lentil lectin-sepharose. radioactivity associated with macromolecules was assayed by precipitation with trichloracetic acid, and that associated with melanoma-associated antigens (maa) was assayed by specific immunoprecipitation with xenogeneic anti-melanoma serum. following the last step ... | 1982 | 7042082 |
| synergistic effect of lentil lectin and antithymocyte serum on rat skin allograft survival. | 1982 | 7046168 | |
| a cautionary note on the use of lentil lectin affinity chromatography in the presence of sodium deoxycholate. | when affinity chromatography with lentil lectin (lch)-sepharose was carried out in 0.5% (w/v) sodium deoxycholate (doc), 10 mm tris, ph 8.2, lch consistently appeared in the 0.1 m alpha-methyl-mannoside (alpha-meman)-eluted fraction. eluted lch was recovered in both the control and specific immunoprecipitates with antisera directed against components of the glycoprotein fraction, suggesting that at least a portion of the lch retained the capacity to interact with protein-bound carbohydrate. we s ... | 1982 | 7057043 |
| purification and characterization of a mannose/glucose-specific lectin from vicia cracca. | the seeds of vicia cracca are known to contain two lectins: an n-acetylgalactosamine-binding lectin, which reacts specifically with human erythrocytes of blood group a, and a mannose/glucose-binding lectin unspecific towards human blood groups. the second lectin is the object of the present work. purification was achieved by affinity chromatography on sephadex g-100. the native lectin has a molecular weight of 44,000. it is composed of two small (mr 5,700) and two large subunits (mr 17,500). bin ... | 1982 | 7060560 |
| red cell membrane protein anomalies in congenital dyserythropoietic anaemia, type ii (hemp as). | in all of six cases of congenital dyserythropoietic anaemia, type ii (hempas), gel electrophoresis in the presence of sds revealed abnormally rapid migration of the preponderant integral membrane protein, band 3. after proteolysis of intact cells, the remaining part of the band 3, comprising the intramembrane segment and the cytoplasmic domain, migrated electrophoretically as a single band, identical in mobility to that from normal cells treated in the same manner. the anomaly thus resides in th ... | 1982 | 7066206 |
| cell surface carbohydrates in crithidia deanei: influence of the endosymbiote. | the cell surface of the symbiote-containing and symbiote-free strains of crithidia deanei were characterized comparatively by using 22 highly purified lectins with specificities for n-acetyl glucosamine, n-acetyl galactosamine, galactose, mannose-like residues, fucose and sialic acid. the specificity of the cell surface carbohydrate in both strains were analyzed by agglutination and lectin-binding assays. c. deanei with or without endosymbiote was specifically agglutinated by lectins from tritic ... | 1982 | 7067702 |
| biosynthesis of gp160, the major trypsin-sensitive surface glycoprotein of macrophages. | the biosynthetic origin was investigated of gp160, the trypsin- and plasmin-sensitive surface glycoprotein of caseinate-elicited macrophages from guinea pigs. biosynthesized surface components were analyzed by reacting [35s]methionine-labeled macrophages with trinitrobenzene sulfonic acid and purifying trinitrophenyl-substituted surface proteins by immunoprecipitation. identification of gp160 was based on its molecular weight and unique trypsin sensitivity. in addition, [35s]methionine-labeled s ... | 1982 | 7076683 |
| lectin-reactive components in white matter membranes from normal and multiple sclerosis brains. | polypeptides derived from human white matter membranes reacted with the radioiodinated lectins concanavalin a, lens culinaris phytohemagglutinin, ricinus communis agglutinin and wheat germ agglutinin after electrophoresis in polyacrylamide pore gradient gels. the molecular weights of these lectin-reactive bands were estimated by comparison with radioiodinated protein standards by using the linear relationship between log of the molecular weight and log of the gel concentration reached by the pro ... | 1982 | 7077325 |
| isolation and characterization of plasma-membrane glycoproteins from pig epidermis. | 1. non-desmosomal plasma membranes enriched in plasma-membrane marker enzymes and in metabolically labelled glycoproteins were isolated on a large scale from up to 500g of pig ear skin slices. sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and periodic acid/schiff staining revealed the presence of four major glycosylated components in the apparent molecular-weight range 150000-80000. 2. a large proportion of the marker enzymes, the d-[(3)h]glucosamine-labelled glycoproteins and the p ... | 1982 | 7082290 |
| intercellular adhesion of rat hepatocytes. identification of a cell surface glycoprotein involved in the initial adhesion process. | a cell surface molecule involved in intercellular adhesion between rat hepatocytes in vitro has been identified. papain-solubilized plasma membrane components were fractionated; the ability of the solubilized components to neutralize the inhibition of cell aggregation, caused by multispecific antibodies directed against the cell surface, was used to follow the purification. the purified components were used to immunize rabbits. the resulting antibodies effectively inhibited hepatocyte aggregatio ... | 1982 | 7085603 |
| lectin-binding characteristics of human natural killer cells. | human natural killer (nk) cells separated initially by density centrifugation of lymphocytes (e+) forming rosettes with sheep red blood cells (srbc), were further fractionated on gradients of bovine serum albumin (bsa). low density fractions contained effector cells which displayed high cytotoxicity against the nk-sensitive erythroleukaemic cell line, k562. these low density cells, which expressed receptors for fc and the monoclonal antibody okmi, showed enhanced cytotoxicity when treated with l ... | 1982 | 7095832 |
| purification of a native membrane-associated adenovirus tumor antigen. | a 15,000-dalton protein was purified from hela cells infected with adenovirus type 2. proteins solubilized from a membrane fraction of lytically infected cells was used as the starting material for purification. subsequent purification steps involved lentil-lectin, phosphocellulose, hydroxyapatite, deae-cellulose, and aminohexyl-sepharose chromatographies. a monospecific antiserum, raised against the purified protein, immunoprecipitated a 15,000-dalton protein encoded in early-region e1b (e1b/15 ... | 1982 | 7097863 |
| fractionation and identification of pig lymph node plasma membrane glycoproteins. | plasma membrane vesicles purified from pig mesenteric lymph node tissue were solubilized in 1% (w/v) sodium deoxycholate and the fractionation of both membrane proteins and glycoproteins assessed by gel filtration on aca 34. milligram quantities of the glycoprotein fraction, consisting of 12 distinct bands on sds-page ranging in apparent mol. wt from 12,000 to 250,000, were purified by affinity chromatography on lentil lectin-sepharose. identified among these by specific immunoprecipitation were ... | 1982 | 7099163 |