Publications

histochemical localization of nitrate reductase.	nadh-dependent nitrate reductase (e.c. 1.6.6.1) was ultrastructurally localized in norflurazon-treated and control soybean cotyledons [glycine max (l.) merr.] by a method based upon the increase in osmiophilia due to the formation of an azo dye. the reaction product was observed in small vesicles throughout the cytoplasm. an apparent transport of nitrite to the plastid, the site of nitrite reduction, may occur through fusion of the nitrite-containing vesicles with the chloroplast envelope. plant ...	1981	7196901
determination of trace amounts of selenium in corn, lettuce, potatoes, soybeans, and wheat by hydride generation/condensation and flame atomic absorption spectrometry.		2013	7196924
food antibodies in milk from guatemalan women.	this study investigates the presence of antibodies against food products in milk samples from 30 guatemalan women of 3 different socioeconomic status: 10 rural poor, 10 urban poor, and 10 urban privileged. the 3 groups had varied dietary habits. both the rural and urban poor mothers do not consume cow's milk. black beans are consumed more often by the urban groups while soy bean products are consumed by both rural and urban poor regularly. milk samples were collected from the mothers. the ...	1981	7196944
age-related changes in transfer rnas and synthetases in germinating soybean (glycine max) cotyledons.	transfer rnas and aminoacyl-trna synthetases were isolated from 5- and 20-day-old etiolated ageing soybean cotyledons. the relative concentrations of mg2+ and atp for optimal acylation (using homologous trnas and synthetases) for the 16 amino acids tested indicate that the amino acids can be grouped into 3 categories with a mg:atp ratio of 2:1, 5:1 and 10:1 (aspartic acid, isoleucine, leucine, serine, and valine). the total amino acid acceptance for 5-day-old cotyledons was 0.427 mumol trna and ...	1981	7197246
studies on lectins. li. the role of mn2+ in the activity of the soybean lectin.	in contradistinction to previous reports, the lectin of soybeans (glycine soja) has been shown to retain its erythroagglutinating activity after complete removal of manganese from its molecule. the applied demetallization procedures (dialysis against 0.1 m hcl or 1m acetic acid and against 0.1 m edta following by dialysis against 1 m acetic acid) had no effect on the stability of the lectin subunit structure, as proved by ultracentrifugation analysis or thin-layer chromatography on sephadex g-20 ...	1981	7197555
the isolation and characterization of a root lectin from soybean (glycine max (l), cultivar chippewa).	a lectin has been isolated from the roots of 5-day soybean (glycine max (l) cultivar chippewa) seedlings, and its properties have been compared to those of the soybean seed lectin. the sugar-binding activities of the two lectins, both in terms of specific hemagglutinating activity and sugar specificity, are indistinguishable. molecular properties of the two lectins, measured as relative molecular weights, isoelectric and electrophoretic patterns, amino acid compositions, immunochemical cross-rea ...	1981	7198118
soybean proteinase inhibitors and human proteolytic enzymes: selective inactivation of inhibitors by treatment with human gastric juice.	the stability of soybean proteinase inhibitors to 0.1 m hcl and to human gastric juice was investigated. purified kunitz soybean inhibitor (sbti), purified lima bean inhibitor (lbi), and crude, aqueous extract (1:5 w/v) of three varieties of soybeans were incubated for 24 hours at 37 degrees. the sbti, the lbi, and the soybean extracts were mixed with gastric juice or 0.1 m hcl in amounts corresponding to 4.0 g sbti, 2.4 g lbi, and 130 g soybean meal per liter. the trypsin and chymotrypsin inhib ...	1981	7198142
effect of microwave heating of soybeans on protein quality.	the purpose of this study was to determine the protein quality of microwave cooked soybeans by the rat growth and protein efficiency ratio method (per). it was found that properly cooked, dry, hulled, whole soybean seeds had a per of 2.4 +/- .06 (mean +/- standard error) and a mean weekly weight gain of 21.2 +/- 1.1 which were equivalent to 2.53 +/- .10 and 18.3 +/- 1.0 g for casein, respectively. these data demonstrate the value of microwave cooked soybeans and suggest further research on the p ...	1981	7199277
the persistence and disappearance by washoff and dryfall of methoxychlor from soybean foliage--a preliminary study.	the persistence and disappearance (washoff or dryfall) of methoxychlor [2,2-bis(p-methoxyphenyl)-1,1,1-trichloroethane] from mature soybean [glycine max (l.) merrill] foliage was investigated in a small field plot study under natural rainfall conditions in 1977 and 1978. residue analyses were conducted using whole plant samples. methoxychlor washoff rate was 8+/- 4% of the amount on plants (prior to rain) per centimeter of rainfall, regardless of time after application. total seasonal washoff fo ...	1981	7200103
availability to rats of zinc from soybean seeds as affected by maturity of seed, source of dietary protein, and soluble phytate.	male rats were used to assess the availability of zinc in soybean seeds (glycine max, var. 'amsoy') as affected by maturity of seed, dietary protein source, and exogenous dietary sodium phytate. seeds were harvested from either 84- (immature) or 120-day-old (mature) plants grown in 65zn-labeled nutrient solutions that contained either 0.066 or 0.262 ppm zinc as zinc sulfate. increased zinc supply to plants increased the zinc content of seeds; immature seeds had higher concentrations of zinc than ...	1982	7200513
[agronomic, nutritional and processing characteristics of soybean germ plasm with colored seed coats].	ten soybean varieties with colored seed coats were evaluated in jaboticabal, with the objective of obtaining information as to color preference in the direct use of soybeans in human consumption. the above-mentioned material showed good adaptation to the local environmental conditions: plant cycles were smaller than the santa rosa (121 days), varying from 107 to 119 days, in spite of the fact that some varieties started blooming later than santa rosa. all materials are within the minimum standar ...	1981	7201302
reduction of the soy-induced inhibition of nonheme iron absorption.	recent studies in normal men have demonstrated that soy products have a pronounced inhibitory effect on the absorption of nonheme dietary iron. the purpose of the present investigation was to explore approaches to overcoming this inhibition. a modest but significant increase in iron absorption was observed when test meals containing either isolated soy protein or whole soybeans were baked at 200 degrees c. the addition of 100 mg ascorbic acid to a meal containing isolated soy protein had a more ...	1982	7201739
ultrastructural localization of urate oxidase in nodules of sesbania exaltata, glycine max, and medicago sativa.	the localization of urate oxidase (=uricase, e.c. 1.7.3.3) was determined cytochemically in nodules of sesbania exaltata (raf.) cory, soybean (glycine max [l.] merr.) and alfalfa (medicago sativa [l.] ), using the precipitation of peroxide (produced during the oxidation of urate) by cerium chloride. cerium perhydroxide reaction product was noted only in the microbodies, a localization consistent with biochemical fractionation studies on urate oxidase. urate oxidase was present not only in the un ...	1982	7201988
in vitro evaluation of fungicides and antibiotics against macrophomina phaseolina (tassi) goid, the incitant of leaf spot of soybean (glycine max.).		1981	7309555
some properties of the lectin from datura stramonium (thorn-apple) and the nature of its glycoprotein linkages.	the lectin from datura stramonium (thorn-apple; solanaceae) has been purified by affinity chromatography and shown to be a glycoprotein containing about 40% (w/w) of carbohydrate. the most abundant amino acids are hydroxyproline, cystine, glycine and serine. results obtained by gel filtration in 6m-guanidinium chloride on sepharose 4b suggest that it has a subunit mol.wt. of about 30000 and that it probably associates into dimers. the lectin is inhibited specifically by chitin oligosaccharides a ...	1981	7325959
[sensitivity of different biological methods for differentiating the protein value of various foods].	the nutritive value of proteins from different sources was studied. the value for per, weight of the animals, iec, npr, ndpcal%, digestibility (d), and biological value (bv) was determined with diets containing 7% of total protein. the best results were obtained for milk, opaque-2 corn, rice and soybeans. the association of rice + soybeans was optimum. the results suggest also that it is advantageous to supplement wheat with cassava. comparison of the results obtained by the different biological ...	1981	7337518
the amino acid sequence of pea (pisum sativum) leghemoglobin.	the amino acid sequence has been determined for leghemoglobin component i from root nodules of pea, pisum sativum. pea leghemoglobin is one polypeptide chain composed of 147 amino acids, it contains one methionine residue at position 144, and three histidines, which are at positions 60, 92 and 101. the sequence has at least seven polymorphic residues, but it was not possible to separate the polymorphic protein forms which had identical electric charge. the approximate molecular weight of pea leg ...	1980	7378476
identification of "nodule-specific" host proteins (nodoulins) involved in the development of rhizobium-legume symbiosis.	infection of legume roots with rhizobium species results in the development of a root nodule structure in which the bacteria form an intracellular symbiosis with the plant. we report here that the infection of soybean (glycine max l.) roots with rhizobium japonicum results in the synthesis by the plant of at least 18-20 polypeptides other than leghemoglobin during the development of root nodules. identification of these "nodule-specific" host polypeptides (referred to as nodulins) was accomplish ...	1980	7388942
effect of raw legume diets on intestinal absorption of d-galactose by chick.	the effect of four raw legume diets on the intestinal absorption of d-galactose and oxygen consumption were studied in chick. field beans (vicia faba), soybeans (glycine soja), bitter vetch (vicia ervilia), and navy beans (phaseolus vulgaris), were used. the intestinal absorption was determined by both in vivo and in vitro techniques. in vivo, only navy beans and soybeans inhibit intestinal transport of d-galactose, while in vitro all the diets do. oxygen consumption by intestinal rings increase ...	1980	7394275
response of young chicks to raw, defatted, kunitz trypsin inhibitor variant soybeans as sources of dietary protein.	two trials, using 264 8-day-old male crossbred chicks, were conducted to evaluate the nutritional value of two trypsin-inhibitor (kunitz) variant soybeans. these two variants, designated pi 157440 (pi) and number 661, have lower trypsin-inhibitor activities than do us commercially grown soybean cultivars such as amsoy 71. raw pi, 661, and amsoy 71 soybeans were defatted and then compared to a commercially processed solvent extracted soybean meal (sbm) using purified diets with soybeans or sbm as ...	1980	7413562
pollutants in breast milk of vegetarians.	in the article by rogan et al. on pollutants in breast milk, the statement that "there are no obvious...dietary predictors" of chemical pollutants in human milk is challenged by recent unpublished data on vegetarian women. we studied 12 woman who were all members of a religious community that eliminates all animal products from the diet and uses soybeans and grains for proteins. samples of breast milk were gathered in august 1979 and were analyzed for 17 chemical substances. the lipid levels ...	1981	7464895
increase of blood group a and loss of blood group sda activity in the mucus from human neoplastic colon.	aqueous extracts of human neoplastic and adjacent normal colonic mucosa were investigated for hemagglutination inhibition of both the anti-a serum and the dolichos biflorus lectin. the anti-a antiserum was inhibited by the extracts from neoplastic tissues to a much higher extent than by that from normal mucosa; the inhibition was strictly dependent on the blood group and the secretor status of the individual. the inhibition titers against the dolichos lectin were much lower for the tumor than fo ...	1980	7466135
interaction of native and asialo rat sublingual glycoproteins with lectins.	the binding properties of the rat sublingual glycoprotein (rsl) and its asialo product with lectins were characterized by quantitative precipitin(qpa) and precipitin inhibition(qpia) assays. among twenty lectins tested for qpa, native rsl reacted well only with artocarpus integrifolia (jacalin), but weakly or not at all with the other lectins. however, its asialo product (asialo-rsl) reacted strongly with many gal and galnac specific lectins-it bound best to three of the galnac alpha 1-->ser/thr ...	1995	7475931
selective inhibition of bitter taste of various drugs by lipoprotein.	previously, we demonstrated that lipoprotein composed of phosphatidic acid (pa) and beta-lactoglobulin (lg) selectively and reversibly suppress the frog taste nerve response to bitter substances. in the present study, we examined the effects of various lipoproteins on the taste sensation to various stimuli in humans by a psychophysical method. among various lipoproteins composed of different of lipids and proteins, the lipoproteins composed of pa and proteins were most effective in suppressing b ...	1995	7479549
soybean trypsin inhibitor(s) reduce absorption of exogenous and increase loss of endogenous protein in miniature pigs.	it was the purpose of this study to define whether trypsin inhibitors impair protein digestibility via enhanced loss of exogenous or endogenous protein by quantifying those losses using the homoarginine technique, recently developed in this laboratory. pigs fitted with permanent ileal t-cannulas were fed test meals containing homoarginine-labeled protein. the meals contained casein and increasing doses of trypsin inhibitors (experiment 1) or alternatively either heat-treated or raw ground soybea ...	1993	7505318
pancreatic enlargement is evident in rats fed diets containing raw soybeans (glycine max) or cowpeas (vigna unguiculata) for 800 days but not in those fed diets based on kidney beans (phaseolus vulgaris) or lupinseed (lupinus angustifolius).	pancreatic weights and composition were studied with rats fed diets containing raw legume seeds for up to 800 d. rapid pancreatic enlargement was induced by dietary soybeans (glycine max) (high kunitz and bowman-birk trypsin inhibitor contents, moderate lectin content) during the initial 150 d. over the next 200 d the rate of pancreatic growth was similar to that in controls. after 350 d a second period of rapid pancreatic growth occurred. macroscopic pancreatic nodules were evident in a number ...	1993	7505319
mutagenic effects of nitropyrenes in a soybean test system.	the mutagenic activity of 1-nitropyrene (1-np), 1,3-dinitropyrene (1,3-dnp), 1,6-dinitropyrene (1,6-dnp) and 1,8-dinitropyrene (1,8-dnp) was assayed in heterozygous soybean plants (y11y11), based on the appearance of mutational spots (yellow, dark green and twin) on the leaves. 1-np, 1,3-dnp, 1,6-dnp and 1,8-dnp were direct-acting mutagens in a soybean test system, and mutagenicity was enhanced by addition of pyrene as a precursor. the mutagenicity of dinitropyrenes was enhanced by pretreatment ...	1994	7506387
echinostoma caproni and e. trivolvis alter the binding of glycoconjugates in the intestinal mucosa of c3h mice as determined by lectin histochemistry.	mouse (c3h) mucosal glycoconjugates were examined in normal small intestines and intestines infected with echinostoma caproni or e. trivolvis using six different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin i (rca-i), glycine max soybean agglutinin (sba), dolichos biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). the expression of lectin-binding sites and the intensity of the binding of ...	1993	7507137
accumulation of reactive oxygen species and oxidation of cytokinin in germinating soybean seeds.	seed germination is an important developmental switch when quiescent seed cells initiate oxidative phosphorylation for further development and differentiation. during early imbibition of soybean seeds (glycine max l. cv. weber), a superoxide dismutase (sod) activity peak was observed, in embryonic axes, after 6 h imbibition. peroxidase activities, including catalase, were significantly increased after 12 h inhibition and during germination phase iii. catalase was the most efficient enzyme in cat ...	1994	7521301
[natural proteinase inhibitors as a basis for creating new drugs].	isolation of the proteinases inhibitors, available for medicinal purposes, was described, where the inhibitor of the kunitz type was obtained from bovine pancreas and the inhibitor of the bowman-birk type from soybeans. screening of the immobilization procedures was carried out, which enabled the authors to produce the polymeric conjugates of the proteinase inhibitors exhibiting the maximal rate of activity against pancreatic proteinases and granulocyte elastases. pharmacokinetics of the protein ...	1994	7521547
differential localization of lectin binding sites and neuropeptides in human dorsal root ganglia.	the subpopulations were compared of neurons in human dorsal root ganglia (drg), as substance p, identified by somatostatin, glycine max lectin (sba) specific to terminal n-acetylgalactosamine, and ulex europaeus i agglutinin (uea-i) specific to l-fucose. the lectins and neuropeptides all bound to neurons of small diameter. furthermore, the majority of the sba binding neurons or somatostatin positive neurons were also uea-i binding neurons. however, sba binding neurons were not colocalized with s ...	1994	7531191
bradyrhizobium liaoningense sp. nov., isolated from the root nodules of soybeans.	seventeen strains of extra-slowly growing (esg) soybean rhizobia isolated from root nodules of glycine soja and glycine max growing in five provinces (liaoning, heilongjiang, shanxi, hubei, and anhui) in the people's republic of china were compared with 48 reference strains belonging to the genera bradyrhizobium, rhizobium, and agrobacterium by performing a numerical analysis of 191 phenotypic features. our results showed that all of the esg strains examined clustered closely in the genus bradyr ...	1995	7547289
sink limitation induces the expression of multiple soybean vegetative lipoxygenase mrnas while the endogenous jasmonic acid level remains low.	the response of individual members of the lipoxygenase multigene family in soybeans to sink deprivation was analyzed. rnase protection assays indicated that a novel vegetative lipoxygenase gene, vlxc, and three other vegetative lipoxygenase mrnas accumulated in mature leaves in response to a variety of sink limitations. these data suggest that several members of the lipoxygenase multigene family are involved in assimilate partitioning. the possible involvement of jasmonic acid as a signaling mol ...	1995	7549487
crystallization and preliminary x-ray investigation of lipoxygenase-3 from soybeans.	soybean lipoxygenase-3 has been crystallized by the vapor diffusion method in 16-20% polyethylene glycol (average m(r), 3,400), 0.2 m sodium acetate buffer, ph 5.7, at 21 degrees c, at a protein concentration of 8-15 mg/ml. the crystals, which diffract to 3-a spacings, belong to the monoclinic space group c2. cell constants are a = 111.9, b = 136.4, and c = 61.6 a and beta = 95.7 degrees. the calculated value of matthews's constant, vm = 2.48 a3/kda, is consistent with the presence of one molecu ...	1995	7549886
glycans with n-acetyllactosamine type 2-like residues covering adult schistosoma mansoni, and glycomimesis as a putative mechanism of immune evasion.	glycans at the surface of adult schistosoma mansoni were investigated with gold-labelled lectins. the fragile complex of the glycans with the outer membranes could be preserved for electron microscopy by avoiding extensive pre-fixation with aldehydes and by introducing osmium-ferrocyanide as a membrane fixative. male and female worms were entirely covered with glycans that intensely bound lectins from erythrina cristagalli and datura stramonium, suggesting that galactose(beta 1-4)n-acetylglucosa ...	1995	7567100
deantigenation of human type b erythrocytes with glycine max alpha-d-galactosidase.	conversion of erythrocyte membrane b antigen to h antigen produces blood type o which is universally transfusable. if efficient large-scale production of enzymatically converted red blood cells is to be achieved, then optimal conditions for deantigenation must be determined. cell suspension assays were used to study the blood group b activity of glycine max (soybean) alpha-d-galactosidase on native human erythrocytes. the enzyme readily hydrolyzed the terminal alpha-d-galactosyl residue of the b ...	1995	7579003
organization of soybean chalcone synthase gene clusters and characterization of a new member of the family.	chalcone synthase (chs; ec 2.3.1.74), the first committed enzyme of the multibranched pathway of flavonoid/isoflavonoid biosynthesis is encoded by a multigene family in soybean, (glycine max l. merrill). our results suggest that this gene family comprises at least seven members, some of which are clustered. we have identified four chs clusters in the allo-tetraploid g. max genome and chs5, a newly characterized member of the chs gene family is present in two of them. we describe the complete nuc ...	1995	7579172
transformation of soybean (glycine max) via agrobacterium tumefaciens and analysis of transformed plants.		1995	7581657
induction of micronucleus formation in mouse splenocytes by the soy isoflavone genistein in vitro but not in vivo.	the effects of genistein (one of the major soybean isoflavones), genistein (the glucosylated form of genistein) and etoposide (a topoisomerase 11 inhibitor) have been studied in mouse splenocytes in culture. genistein (25 microm), genistein (25 microm) and etoposide (0.1 microm) all induced the production of large numbers of micronuclei; however, genistein at 12.5 or 2.5 microm had no clastogenic effect. in a second study, mice were gavaged with 20 mg genistein/kg body weight/day for 5 days (app ...	1995	7590538
distribution of lectin binding in the testes of the musk shrew, suncus murinus.	distribution of lectin binding in the testis of the musk shrew, suncus murinus, was investigated by light and transmission electron microscopy. not only spermatogenic cells but also sertoli cells bound some lectins. canavalia ensiformis agglutinin (con a) and wheat germ agglutinin (wga, triticum vulgaris), indicating the presence of d-mannose and n-acetyl-d-glucosamine respectively, showed an intense reaction in the acrosomal region of early to late spermatids. ricinus communis agglutinin i (rca ...	1995	7591995
the effect of roasting temperature applied to whole soybeans on site of digestion by steers: i. organic matter, energy, fiber, and fatty acid digestion.	a 4 x 5 youden square design was used to determine the effects of form of oil and roasting temperature of soybeans on site and extent of om, fiber, energy, lipid, and fatty acid digestion and the degree of ruminal biohydrogenation of c18 unsaturated fatty acids. four steers (373 kg +/- 30.7) with ruminal, duodenal, and ileal cannulas were fed each of five diets during five periods. diets contained (percentage of dm) corn silage (50%), alfalfa hay (24%), and a corn-urea concentrate (6%). cornstar ...	1995	7592100
the effects of roasting temperature applied to whole soybeans on site of digestion by steers: ii. protein and amino acid digestion.	a 4 x 5 youden square design was used to determine the effect of roasting temperature of whole soybeans on escape of cp from the rumen and disappearance of n from the small intestine in steers. four steers (average bw 373 kg +/- 30.7) cannulated at the rumen, duodenum, and ileum were fed each of five diets (1.8% of bw) over five periods. the basal diet contained corn silage (50% of diet dm), alfalfa hay (24%), corn-urea premix (6%), corn starch grits (16.6%), and soybean oil (3.4%). soybeans (16 ...	1995	7592101
[histochemical study of biotinylated lectins in prostatic cancer].	we examined the staining pattern of various lectins in the malignant and benign prostatic tissues to clarify the difference of sugar residues of mucosubstances between them. the binding of different lectins (concanavalia ensiformis [con a], triticum vulgaris [wag], ricinus communis [rca-i], arachis hypogaea [pna], ulex europaeus [uea-i], glycine max [sba], and gliffonia simplicifolia ii [gsa-ii]) to cells of benign prostatic tissues (17 cases) and adenocarcinoma (54 cases) was evaluated immunohi ...	1995	7596076
effect of dietary raw soybeans on coccidiosis in chickens.	six battery experiments determined the effect of feeding raw soybeans (rsb) in corn-soybean diets on coccidiosis in broiler chickens. the experimental design represents a 2 x 3 factorial arrangement of treatments with three dietary levels of incorporation of rsb (0, 50, and 100%), and chickens either infected or uninfected. the coccidial species tested singularly were eimeria acervulina, eimeria maxima, eimeria necatrix, eimeria brunetti, and eimeria tenella. with all species of eimeria a signif ...	1995	7603956
purification and characterization of an nad(p)h:quinone oxidoreductase from glycine max seedlings.	an nad(p)h:(quinone acceptor) oxidoreductase (ec 1.6.99.2) was purified from glycine max seedlings by means of chromatographic procedures. after 1371-fold purification, the enzyme showed a single band in ief corresponding to an isoelectric point of 6.1. a single band was also found in native-page both by activity staining and coomassie brilliant blue staining. the molecular mass determined in sds-page was 21900 da, while in hplc gel-filtration it was 61000 da. the nad(p)h:quinone oxidoreductase ...	2013	7603972
two classes of cortical neurones labelled with vicia villosa lectin in the guinea-pig.	cell surface substance(s) containing terminal n-acetylgalactosamine (gainac) was localized in the cerebral cortex of the guinea-pig. vicia villosa and glycine max, both specific to gainac, labelled the surface of the cell body of multipolar and triangular neurones. the labelling extended to the apical dendrite and axon initial segment in the triangular neurones. they were distributed exclusively in layer 5. most of the labelled multipolar neurones contained a calcium-binding protein, parvalbumin ...	1995	7605912
effect of protein and starch degradation rates on rumen metabolism in taiwan native goat.	six ruminal and duodenal cannulated taiwan native goats (body weight = 20 kg) were fed 35% roughage, 65% concentrate diet (crude protein = 9.5%) in a 6 x 6 latin square design to study the effect of the combination of 3 varying starch (corn) and 2 varying protein (soybeans) ruminal degradation rates on ruminal microbial density and ruminal nutrient digestibilities. goats in treatment consisting of both rapid starch and protein ruminal degradation rates had higher total bacterial and protozoal nu ...	1995	7624449
changes in the iron coordination sphere of fe(ii) lipoxygenase-1 from soybeans upon binding of linoleate or oleate.	fe k-edge x-ray absorption spectra of the non-heme iron constituent of lipoxygenase-1 from soybeans were obtained. the spectrum of 2.5 mm fe(ii) lipoxygenase, mixed with 1.2 m linoleate in the absence of o2, was compared to the spectrum of the native (i.e. untreated) enzyme. in the lipoxygenase-linoleate complex, an edge shift to lower energy was observed. this indicated that the iron-ligand distances in this complex are slightly longer than those in the untreated enzyme species. the extended x- ...	1995	7628469
the soybean gh2/4 gene that encodes a glutathione s-transferase has a promoter that is activated by a wide range of chemical agents.	transcriptional activation of the soybean (glycine max) gh2/4 gene (also referred to as gmhsp26-a) and increase in abundance of the gh2/4 mrna (also referred to as pce54) have been previously shown to occur following treatment of soybean seedlings with auxins, nonauxin analogs, heavy metals, and a variety of other agents. to determine whether the gh2/4 promoter is responsive to an array of different agents, we have analyzed the inducibility of the gh2/4 promoter fused to the beta-glucuronidase r ...	1995	7630972
the utilization of lupin (lupinus angustifolius) and faba bean globulins by rats is poorer than of soybean globulins or lactalbumin but the nutritional value of lupin seed meal is lower only than that of lactalbumin.	the effects of dietary sweet lupin (lupinus angustifolius, unicrop) seed meal or its insoluble fiber (nonstarch polysaccharides + lignin) on performance, digestibility and nitrogen utilization in growing rats were studied in four experiments. globulin proteins isolated from lupin, faba bean (vicia faba l. minor) or soybean (glycine max) were also incorporated into purified diets as replacements for lactalbumin (control) and the nutritional effects were evaluated. isocaloric, legume-based diets s ...	1995	7643249
genetic damage and the inhibition of 7,12-dimethylbenz[a]anthracene-induced genetic damage by the phytoestrogens, genistein and daidzein, in female icr mice.	populations consuming soybeans have reduced rates of breast, colon and prostate cancer possibly due, in part, to the presence in soybeans of two estrogenic isoflavones, genistein and daidzein. this study investigated the genotoxicity of these soya isoflavones and their interactions with 7,12-dimethylbenz[a]anthracene (dmba)-induced sister chromatid exchanges (sce) in bone marrow cells and dna adduct formations in liver and mammary glands of mice. groups of female icr mice were pretreated i.p. wi ...	1995	7656220
effects of gamma irradiation on mycotoxin disappearance and amino acid contents of corn, wheat, and soybeans with different moisture contents.	the effects of gamma irradiation on degradation of aflatoxin b1 in wheat, corn, and soybeans and of t-2 toxin in wheat, deoxynivalenol (don) in soybeans, and zearalenone in corn at 9, 13, and 17% moisture were studied. radiation doses of 5, 7.5, 10 or 20 kgy were applied to spiked grain samples, and the residual toxins were measured using an enzyme linked immunosorbent assay (elisa). irradiation does of up to 20 kgy did not significantly affect aflatoxin b1 in any of the three grains, but signif ...	1995	7659700
procedures for measuring the quality of heat-treated soybeans.	soybeans were subjected to 12 heat treatments, differing in temperature and steeping (conditioning) time: control (no heat); 110 degrees c, 0 min; 110 degrees c, 30 min; 123 degrees c, 0 min; 123 degrees c, 30 min; 135 degrees c, 0 min; 135 degrees c, 30 min; 146 degrees c, 0 min; 146 degrees c, 15 min; 146 degrees c, 30 min; 153 degrees c, 30 min; and 160 degrees c, 30 min. these soybean treatments were tested for protein dispersibility index, absorbance at 420 nm, adin, pepsin-insoluble n, and ...	1995	7673525
deletion analysis and localization of sbprp1, a soybean cell wall protein gene, in roots of transgenic tobacco and cowpea.	sbprp1 is a member of the soybean (glycine max l. merr) proline-rich cell wall protein family and is expressed at high levels in root tissue. to characterize the sequences required for this expression, we have fused 1.1 kb of upstream flanking dna sequence from an sbprp1 genomic clone to a gene encoding beta-glucuronidase (gus). this construct was introduced into tobacco using agrobacterium tumefaciens-mediated transformation. histochemical staining of gus activity in transgenic tobacco indicate ...	1993	7678758
comparison of the mast chemiluminescent assay system with rast and skin tests in allergic children.	the mast chemiluminescent assay (cla) system uses enzyme-linked antihuman ige and a chemiluminogenic substance to determine specific ige antibodies. the mast cla system therefore not only makes unnecessary the precautions required for handling isotopic materials but has the advantage of long-term reagent stability. the mast cla system assay protocol consists of three steps: overnight incubation of serum, a 4-hour incubation with enzyme-labeled antibody, and a 30-minute chemiluminescent reaction, ...	1993	7679259
postnatal development and localization of an n-acetylgalactosamine containing glycoconjugate associated with nonpyramidal neurons in cat visual cortex.	we have analyzed the appearance of n-acetylgalactosamine containing glycoconjugates by staining sections of fixed cat visual cortex with the lectins from vicia villosa (vva) and glycine max (sba) conjugated to fluorescent labels, horseradish peroxidase, or biotin. the appearance of lectin staining during postnatal development followed an inside-out gradient starting in the deep cortical layers at the fourth postnatal week, successively including more of the superficial layers during the first th ...	1993	7681454
[cysteine proteinase inhibitors from soy seeds].	protein inhibitors of cysteine proteinases possessing unusual properties have been found in soya (glycine max) seeds. one of the inhibitor forms has also been detected in bowman-birk inhibitor preparations (both commercial and purified by affinity chromatography on chymotrypsin-sepharose ones). a peculiarity of the inhibitors is that they irreversibly lose their activity in the presence of reducing agents; therefore their effects are normally unobserved under standard conditions of cysteine prot ...	1995	7696428
interaction of the soybean (glycine max) seed lectin with components of the soybean protein body membrane.	in leguminosae, lectins occur in the protein bodies which are membrane-lined storage organelles. studies of the interaction between lectins and other protein body components could provide information about the internal organization of protein bodies and give indication of the biological function of lectins. from soybean (glycine max) seeds, protein bodies and protein body membranes therefrom were isolated. by a technique similar to the elisa procedure, it was shown that the membranes interact wi ...	1994	7710699
x-ray crystal structure of the soybean agglutinin cross-linked with a biantennary analog of the blood group i carbohydrate antigen.	soybean agglutinin (sba) (glycine max), which is a tetrameric galnac/gal-specific lectin, has recently been reported to form unique, highly organized cross-linked complexes with a series of naturally occurring and synthetic multiantennary carbohydrates with terminal galnac or gal residues [gupta, d., bhattacharyya, l., fant, j., macaluso, f., sabesan, s., & brewer, c. f. (1994) biochemistry 33, 7495-7504]. in order to elucidate the nature of these complexes, the x-ray crystallographic structure ...	1995	7711015
characterization of glycoconjugate expression during development of meckel's cartilage in the rat.	the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ...	1995	7717533
immunoaffinity purification and comparison of allantoinases from soybean root nodules and cotyledons.	allantoinase (allantoin amidohydrolase, ec 3.5.2.5) catalyzes the conversion of allantoin to allantoic acid in the final step of ureide biogenesis. we have purified allantoinase more than 4000-fold by immunoaffinity chromatography from root nodules and cotyledons of soybean (glycine max [l] merr.). we characterized and compared properties of the enzyme from the two sources. seed and nodule allantoinases had 80% identity in the first 24 amino acid residues of the n terminus. two-dimensional gel e ...	1995	7724672
native and asialo-tamm-horsfall glycoproteins as important ligands for the detection of galnac beta 1-->and gal beta 1-->4glcnac active lectins.	the binding properties of human tamm-horsfall sd(a+) urinary glycoprotein(thgp) and asialo-thgp with various applied lectins was investigated by quantitative precipitin and precipitin inhibition assays. both glycoproteins completely precipitated abrus precatorius agglutinin(apa). they also reacted well with wistaria floribunda (wfa), glycine max (soybean, sba), and ricinus communis agglutinins and precipitated over 78% of the lectin nitrogen added, but reacted poorly or weakly with all alpha-ano ...	1995	7726822
changes in glycan distribution within the porcine interhaemal barrier during gestation.	changing patterns of glycan distribution are described in porcine placentae at 15, 19, 26, 43, 58, 69 and 109 days gestation, using a carefully selected panel of lectins that allowed partial analysis of saccharide classes and sequences. the lectins used were from galanthus nivalis, pisum sativum, phaseolus vulgaris (leukohaemagglutinin), triticum vulgaris, tetragonolobus purpureus, ulex europaeus-1, arachis hypogaea, erythrina cristagalli, glycine max, maclura pomifera, wisteria floribunda, doli ...	1995	7736552
cloning, nucleotide sequence and expression of the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max.	cdnas encoding the bifunctional dihydrofolate reductase-thymidylate synthase from glycine max were isolated and sequenced. the 1794 base full length cdna contains a single open reading frame of 1593 bases. the predicted size of the encoded protein is 530 amino acids with a molecular weight of 59,707. the protein has two domains: a 226 residue dhfr domain in the n-terminus, which is over 30% identical to human dhfr or the dhfr domain of protozoal dhfr-ts, and a 304 residue thymidylate synthase (t ...	1995	7742362
cdna cloning for a putative cysteine proteinase from developing seeds of soybean.	cdna clones for a putative cysteine proteinase were isolated from developing cotyledons of soybean (glycine max.) using pcr-based techniques. the full-length clone of 1441 bp encodes a proteinase pre-propolypeptide of 380 amino acids. it belongs to the commonly known papain family and shows the highest sequence homology (up to 53% identity) to the protein 15a, a turgor-responsive cysteine proteinase of pea, as well as to several other stress inducible proteinases. biosynthesis of the correspondi ...	1995	7742372
isolation and characterization of a novel immunomodulating fraction from soybeans.	a novel immunomodulating fraction, sp-maf1, was isolated from soybeans as a polysaccharide-protein complex by hot water extraction followed by acid treatment, ethanol treatment, and chromatography on a deae-sepharose cl-6b column, and was characterized in some biological activities. sp-maf1 increased glycolysis and il-1 production by macrophages, but did not affect tnf production. sp-maf1 also increased splenocyte proliferation that was induced by lps and cona, and cona-induced production of il- ...	1993	7763539
physiological and biochemical characterization of glyoxalase i, a general marker for cell proliferation, from a soybean cell suspension.	using a strictly auxin-dependent soybean (glycine max (l.) merr.) cell suspension, we studied the correlation of auxin-dependent cell proliferation and the activity of glyoxalase i (s-lactoylglutathione-lyase ec 4.4.1.5), and enzyme generally associated with cell proliferation in animal, microbial and, as reported recently, also plant systems. we found the activity of glyoxalase i to be modulated during the proliferation cycle, with a maximal activity between day 2 and day 4 of culture growth. a ...	1993	7763577
characterization of chilling-acclimation-related proteins in soybean and identification of one as a member of the heat shock protein (hsp 70) family.	through a 5-d exposure at 14 degrees c/8 degrees c (day/night), soybean (glycine max [l.] merr.) was acclimated to a lower temperature of 8 degrees c. in order to assess changes in protein synthesis related to chilling acclimation, proteins were labeled in vivo with [35s]methionine, separated by two-dimensional gel electrophoresis, and the derived autoradiograms were subjected to computer analysis. two sets of chilling-acclimation-related proteins were characterized following exposure and labeli ...	1993	7763662
genetic and chemical polymorphisms of saponins in soybean seed.	the variation in saponin composition in soybean seeds is explained by different combinations of five genes controlling the utilization of soyasapogenol glycosides as substrates. the function of these genes is variety-specific and organ-specific. phenotypes of over 1000 soybeans were classified into eight saponin types, and the frequency of phenotypes was different between the cultivated [glycine max (l.) merr.] and the wild soybean (g. soja sieb. & zucc.). the aabc saponin type predominated in g ...	1993	7764284
simultaneous induction of postabscission and germination mrnas in cultured dicotyledonous embryos.	cloned mrnas identify three programs of gene expression in cotton (gossypium hirsutum l.) embryos that are associated with the maturation (reserve accumulation) stage, the postabscission stage, which is marked by expression of late-embryogenesis-abundant (lea) mrnas, and germination (broadly defined as including all events through early postgerminative growth). in order to test if the regulation of these programs is the same in other dicotyledonous species, their expression was studied in normal ...	1994	7764404
expression of desiccation-induced and lipoxygenase genes during the transition from the maturation to the germination phases in soybean somatic embryos.	transitions between developmental programs during plant embryogenesis are usually characterized by differential gene expression. soybean (glycine max (l.) merr.) somatic embryos from embryogenic suspension cultures germinate poorly on a germination medium even after being grown on medium containing abscisic acid until developmental changes are induced by desiccation. the objectives of this study were to investigate: (i) the relationship between water loss and germination of somatic embryos, (ii) ...	1994	7764927
purification and molecular characterization of a soybean polygalacturonase-inhibiting protein.	a polygalacturonase-inhibiting protein (pgip) was detected in soybean (glycine max (l.) merr.) seedlings. the protein was purified from germinating seeds and appeared to consist of at least three components with very close molecular weights (between 37 and 40 kda) but each showing a unique n-terminal sequence. primers specific for n-terminal and c-terminal nucleotide sequences of field bean (phaseolus vulgaris l.) pgip were used in a polymerase chain reaction (pcr) on soybean dna, and only one a ...	1994	7765794
binding-protein expression is subject to temporal, developmental and stress-induced regulation in terminally differentiated soybean organs.	binding protein (bip) is a widely distributed and highly conserved endoplasmic-reticulum luminal protein that has been implicated in cotranslational folding of nascent polypeptides, and in the recognition and disposal of misfolded polypeptides. analysis of cdna sequences and genomic blots indicates that soybeans (glycine max l. merr.) possess a small gene family encoding bip. the deduced sequence of bip is very similar to that of other plant bips. we have examined the expression of bip in severa ...	1995	7766051
isolation and characterization of gmsti, a stress-inducible gene from soybean (glycine max) coding for a protein belonging to the tpr (tetratricopeptide repeats) family.	in close vicinity of two fus nuclear genes (chloroplast-specific translation elongation factor cef-g) of soybean (glycine max) we localized a split nuclear gene coding for a protein with tetratricopeptide repeats (tpr). a full-length cdna was sequenced (1871 nucleotides). it encodes a protein (569 amino acids) with high sequence identity to the yeast sti1 stress-inducible and the human transformation-sensitive ief ssp 3521 protein which both carry tpr elements. the soybean gene is heat-inducible ...	1995	7766904
lectin histochemistry of normal and herpesvirus-infected bovine nasal mucosa.	proliferation of pasteurella haemolytica serotype 1 in the nasal cavity following stress or viral infection is an important event in the pathogenesis of bovine pneumonic pasteurellosis. enhanced adhesion of p. haemolytica to nasal mucosa could be one factor that predisposes animals to this proliferation. nasal mucosa from normal and bovine herpesvirus-1 (bhv1)-infected cattle were examined histochemically for their glycoconjugate composition. twenty lectins were screened, six of which were chose ...	1995	7771054
purification and characterization of protein disulfide isomerase from soybean.	protein disulfide isomerase (pdi), which catalyses the folding of newly synthesized or denatured proteins through correct disulfide formation, was purified from soybean (glycine max). the enzyme was purified 12,000-fold over crude extracts to apparent homogeneity in six purification steps: 60-70% ammonium sulfate fractionation, and chromatography on deae toyopearl 650m, q-sepharose fast flow, hiload superdex 200 pg, phenyl sepharose hp, and tsk g-3000 sw. the native enzyme had a molecular weight ...	1995	7775391
estrous cycle-related alterations in the expression of glycoconjugates and lectins in the mouse endometrium shown histochemically.	alterations of the hormonal status may influence diverse cell features relevant to intra- and intercellular communication. we studied histochemically the expression of glycans and endogenous sugar-binding proteins (endolectins) in the mouse endometrium during the estrous cycle. the avidin-biotin-peroxidase complex (abc) technique was used on paraffin sections with a panel of biotinylated lectins and neoglycoproteins. stage-specific changes were observed with galanthus nivalis agglutinin (gna), u ...	1995	7778095
how to reduce the risk factors of osteoporosis in asia.	osteoporosis can be predicted to be a new burden to public health in asia. currently, the incidence of osteoporosis-related fractures is lower there than in most western communities. by the year 2050, however, 50% of the 6.3 million hip fractures which occur worldwide will be in asians as a result of an aging population, a decrease in physical activity and westernization of lifestyles. the cost of treatment and cure of these patients will be enormous, a sufficient financial burden to consume cur ...	1995	7780876
narbonin, a novel 2s protein from vicia narbonensis l. seeds: cdna, gene structure and developmentally regulated formation.	cdna and genomic clones encoding narbonin, a 2s globulin from the seed of narbon bean (vicia narbonensis l.), were obtained using the polymerase chain reaction (pcr) and sequenced. the full-length cdna as well as genomic clones contain a single open reading frame (orf) of 873 bp that encodes a protein with 291 amino acids comprising the mature narbonin polypeptide (m(r) ca. 33 100) and an initiation methionine. the deduced amino acid sequence lacks a transient n-terminal signal peptide. the geno ...	1995	7787188
alpha-subunit of beta-conglycinin, an allergenic protein recognized by ige antibodies of soybean-sensitive patients with atopic dermatitis.	one of the major allergenic proteins in the soybean 7s-globulin fraction, which was recognized by sera of about 25% soybean-sensitive patients with atopic dermatitis, was identified as alpha-subunit of beta-conglycinin. the ige antibodies recognizing the alpha-subunit did not show a cross-reactivity against both alpha'- and beta-subunits known to be highly homologous to alpha-subunit, and also against other allergenic components identified in soybeans [ogawa et al., j. nutr. sci. vitaminol., 35, ...	1995	7787297
normal protein and glycoprotein profiles of reflex tears and trace element composition of basal tears from heavy and slight deposits on soft contact lenses.	we investigated the hypothesis that lacrimal component accumulation on soft contact lenses (scl) may be induced by an abnormal protein, glycoprotein, or trace element composition of the tear fluid. individual tear samples were collected from healthy non-scl wearers (normal patients) and scl wearers grouped as either "slight-depositor" or "heavy-depositor" following scl spoilage rate and frequency. the reflex tear proteins were analyzed by three electrophoretic procedures: sodium dodecyl sulfate- ...	1995	7796521
galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea in capsular glaucoma. a lectin histochemical study.	the aim of the study was to compare galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea with exfoliation material in capsular glaucoma.	1995	7797082
a new s-adenosylmethionine decarboxylase from soybean axes.	a new active s-adenosylmethionine decarboxylase (ec 4.1.1.50) (samdc ii) was extracted from soybean (glycine max) axes. the enzyme was purified to homogeneity by ammonium sulfate fractionation, deae-sepharose and methylglyoxalbis(guanylhydrazone) (mgbg)-sepharose 6b chromatographies. the molecular weight of the native enzyme was 110,000, while the subunit molecular weights were 66,000 and 58,000, indicating a heterodimeric structure. the km value of the enzyme for s-adenosylmethionine was 16 mic ...	1994	7803479
the normal mycoflora of commodities from thailand. 2. beans, rice, small grains and other commodities.	part of a comprehensive study of fungi occurring in commodities in thailand, this paper reports results from 276 samples of mung beans, rice, sorghum and soybeans as well as other minor crops. samples for major commodities were taken from farmers' stocks, middlemen and retail outlets, while those for minor crops were mostly from retail. each sample was divided into two portions, one being examined in bangkok and the second at north ryde. mycological examinations were carried out by direct platin ...	1994	7811571
release of free fatty acids from raw or processed soybeans and subsequent effects on fiber digestibilities.	two in vitro experiments were conducted to determine the rates of lipolysis and the extent of biohydrogenation of fat from raw or processed soybeans and to examine the subsequent effects on fiber digestibilities. in experiment 1, substrates containing soy oil, raw soybeans, extruded soybeans, and soybeans roasted at 132, 146, or 163 degrees c were incubated with ruminal contents for 2, 4, 6, 12, or 24 h; and release of ffa was measured. the ffa released from substrates containing soy oil, extrud ...	1994	7814716
evaluation of a new high protein variety of soybeans as a source of protein and energy for dairy cows.	twenty holstein cows in midlactation were used in a latin square design to evaluate the nutritional quality of a high protein soybean (cp 45%) fed raw or roasted. treatments were 1) control (soybean meal); 2) conventional soybean (maple isle), raw; 3) conventional soybean, roasted; 4) high protein soybean (ac proteus), raw; and 5) high protein soybean, roasted. diets were formulated to be isonitrogenous and consisted of approximately 10% whole crop barley silage, 15% corn silage, 19% alfalfa sil ...	1994	7814730
a serine protease in soybean seeds that acts specifically on the native alpha subunit of beta-conglycinin.	a proteolytic activity directed against the alpha subunit of beta-conglycinin was detected in resting mature seeds of the soybean [glycine max (l.) merrill] cultivar keburi. the relationship between ph and activity and the effect of protease inhibitors revealed that the enzyme was a neutral/alkaline serine protease. the proteolysis of the alpha subunit of beta-conglycinin yielded a specific product with a molecular weight of about 47,000, as determined by sds-page, but the enzyme had no activity ...	1994	7820374
lectin-histochemical analysis of glycans in ovine and bovine near-term placental binucleate cells.	chorionic binucleate cells (bnc) occur in several ruminants including cow, deer, goat and sheep. they migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. we have compared the bnc of near-term, resin-embedded, ovine and bovine placentae using 15 biotinylated lectins and an avidinperoxidase revealing system. there was pronounced conservation of saccharides between the two species. several sub-types of n-gl ...	1994	7850870
consumption of diets containing raw soya beans (glycine max), kidney beans (phaseolus vulgaris), cowpeas (vigna unguiculata) or lupin seeds (lupinus angustifolius) by rats for up to 700 days: effects on body composition and organ weights.	feeding trials have been done with rats to assess the effects of long-term (700 d) consumption of diets based on raw cowpeas (vigna unguiculata; moderate bowman-birk inhibitor content, low lectin content), lupin seeds (lupinus angustifolius; low lectin and protease inhibitor content) or soya beans (glycine max; high kunitz inhibitor content, moderate bowman-birk inhibitor content, moderate lectin content) or diets containing low levels of raw kidney bean (phaseolus vulgaris; high lectin content, ...	1995	7857911
role of chemopreventers in human diet.	recent research has confirmed that many common foods contain nonnutritive components that may provide protection against chronic disease including some forms of cancer. these naturally occurring compounds, which possess anticarcinogenic and other beneficial properties, are referred to as chemopreventers. the predominant mechanism of their protective action is due to their antioxidant activity and the capacity to scavenge free radicals. among the most investigated chemopreventers are some vitamin ...	1994	7867212
duodenase, a new serine protease of unusual specificity from bovine duodenal mucosa. purification and properties.	in this paper, data are presented on purification and properties of a new serine endopeptidase (duodenase) isolated from bovine duodenum mucosa. the enzyme has been purified to homogeneity by combinations of ammonium sulphate fractionation, carboxymethyl-cellulose 52 chromatography, and affinity chromatography on sepharose 4b with kunitz soybean trypsin inhibitor as a ligand. some physicochemical properties of this protease have been investigated. the molecular mass of the purified duodenase was ...	1995	7867648
[influence of foods on the posterior longitudinal ligament of the cervical spine and serum sex hormones].	to investigate the involvement of sex hormones as a potential cause of ossification in the posterior longitudinal ligament (opll) of the cervical spine, we experimentally examined the influence of ingested foods on the serum levels of sex hormones and changes in the spinal ligament. castrated rabbits were administered a sex hormone and raised with special feed. assay of the serum sex hormone levels, analysis of the carbon and nitrogen isotope ratios in body hairs, and histological studies were c ...	1994	7876685
dietary soybean may be antiestrogenic in male mice.	we tested whether dietary soybeans alter prostatic growth and development of prostatic dysplasia in mice that were treated with a synthetic nonsteroidal estrogen, diethylstilbestrol during the first 3 d after birth. soybeans were chosen because they contain substantial amounts of isoflavonic estrogens. the presence of estrogenic isoflavonoids in soybean-containing feed was confirmed by measuring the excretion of seven different plant estrogens in the urine of normal adult male mice. estrogenicit ...	1995	7876918
the effects of phytoestrogens on neonatal rat uterine growth and development.	phytoestrogens found in clover, alfalfa, and soybeans have caused reproductive toxicity in several mammalian species. other estrogens, such as diethylstilbestrol (des), are developmental toxicants, reducing uterine estrogen receptor (er) concentration, altering uterine growth, and eliciting reproductive tract abnormalities in the rat. the present study examines the effects of the phytoestrogens coumestrol and equol on the developing rat uterus. various doses of these compounds were injected sc o ...	1995	7878071
[the human spleen in idiopathic thrombocytopenic purpura].	idiopathic (autoimmune) thrombocytopenic purpura (itp, aitp) represents a relatively frequent impairment. it involves a syndrome of various diseases with a shortened thrombocytes survival caused by anti-platelet antibodies. the majority of cases are of secondary character. spleenectomy often evokes a complete remission of thrombocytopenia. the study describes morphologic findings in spleens of 30 patients with the clinical diagnosis of itp/aitp. the findings were gained by light microscopy from ...	1994	7882065
effect of dietary canola on fatty acid composition of bovine adipose tissue, muscle, kidney, and liver.	the purpose of this study was to determine effects of feeding canola and soybean products as protein supplements on fatty acid composition of adipose tissue and muscle of slaughter bulls and steers and on fatty acid composition of kidney and liver total lipids of bulls. products included canola meal (cm), extruded canola (ec, full-fat), ground canola (gc, full-fat), soybean meal (sbm), and extruded soybeans (es, full-fat). tissues were obtained at slaughter from 75 crossbred beef bulls and 50 cr ...	1994	7883635
modern applications for an ancient bean: soybeans and the prevention and treatment of chronic disease.	soybeans have played an integral part in asian culture, both as a food and as a medicine, for many centuries. in the west, soybeans are best known for their protein content but increasingly, soyfoods are being recognized as having potential roles in the prevention and treatment of chronic diseases, most notably cancer and heart disease. there are also potential roles for soyfoods with respect to osteoporosis and kidney disease. although more research is needed, the existing database on the healt ...	1995	7884533
traditional soyfoods: processing and products.	although soyfoods have been consumed for more than 1000 years, only for the past 15 years have they made an inroad into western cultures and diets. soyfoods are typically divided into two categories: nonfermented and fermented. traditional nonfermented soyfoods include fresh green soybeans, whole dry soybeans, soy nuts, soy sprouts, whole-fat soy flour, soymilk and soymilk products, tofu, okara and yuba. traditional fermented soyfoods include tempeh, miso, soy sauces, natto and fermented tofu an ...	1995	7884535
soy protein products: processing and use.	soy protein products are mainly used as ingredients in formulated foods and seldom are seen by the public. they consist of four broad categories. (1) most soy proteins are derived from "white flakes," made by dehulling, flaking and defatting soybeans by hexane extraction. these may then be milled into defatted flours or grits containing approximately 50-54% protein; extracted with ethanol or acidic waters to remove flavor compounds and flatulence sugars, producing soy protein concentrates contai ...	1995	7884536
compositional changes in trypsin inhibitors, phytic acid, saponins and isoflavones related to soybean processing.	soybeans are high in protein but also contain a number of minor constituents traditionally considered to be antinutritional factors. these include trypsin inhibitors, phytic acid, saponins and isoflavones. these compounds are now thought to have beneficial biological effects in the diet, such as lowering blood cholesterol or preventing cancer. soybean processing changes the content of these minor constituents in various ways. this review discusses the changes in content of trypsin inhibitors, ph ...	1995	7884537