Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| use of serological and mucosal immune responses to mycoplasma hyopneumoniae antigens p97r1, p46 and p36 in the diagnosis of infection. | currently available elisas used to diagnose mycoplasma hyopneumoniae infection in pigs have high specificity but low sensitivity. to develop more sensitive assays, the kinetics of specific serum igg and respiratory mucosal siga responses against three m. hyopneumoniae antigens, namely, p97r1 (an adhesin protein), p46 (a membrane protein), and p36 (a cytosolic protein), were characterised over 133 days following experimental infection. immunoglobulin g against the three proteins remained at high ... | 2014 | 25066030 |
| the mycoplasma hyopneumoniae recombinant heat shock protein p42 induces an immune response in pigs under field conditions. | enzootic pneumonia (ep), resulting from mycoplasma hyopneumoniae infection is one of the most prevalent diseases in pigs and is a major cause of economic losses to the swine industry worldwide. ep is often controlled by vaccination with inactivated, adjuvanted whole-cell bacterin. however, these bacterins provide only partial protection and do not prevent m. hyopneumoniae colonization. attempts to develop vaccines that are more efficient have made use of the recombinant dna technology. the objec ... | 2014 | 25082621 |
| local and systemic immune responses induced by a recombinant chimeric protein containing mycoplasma hyopneumoniae antigens fused to the b subunit of escherichia coli heat-labile enterotoxin ltb. | a multi-antigen chimera composed of three antigens of mycoplasma hyopneumoniae (r1, p42, and nrdf) and the mucosal adjuvant escherichia coli heat-labile enterotoxin b subunit (ltb) was constructed, and its antigenic and immunogenic properties were evaluated in mice and pigs. in addition, we compared the effect of the fusion and co-administration of these proteins in mice. antibodies against each subunit recognized the chimeric protein. intranasal and intramuscular immunization of mice with the c ... | 2014 | 25091529 |
| transcription analysis of the porcine alveolar macrophage response to mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae is considered the major causative agent of porcine respiratory disease complex, occurs worldwide and causes major economic losses to the pig industry. to gain more insights into the pathogenesis of this organism, the high throughput cdna microarray assays were employed to evaluate host responses of porcine alveolar macrophages to m. hyopneumoniae infection. a total of 1033 and 1235 differentially expressed genes were identified in porcine alveolar macrophages in response ... | 2014 | 25098731 |
| effect of feed restriction on performance and postprandial nutrient metabolism in pigs co-infected with mycoplasma hyopneumoniae and swine influenza virus. | as nutritional status and inflammation are strongly connected, feeding and nutritional strategies could be effective to improve the ability of pigs to cope with disease. the aims of this study were to investigate the impact of a feed restriction on the ability of pigs to resist and be tolerant to a coinfection with mycoplasma hyopneumoniae (mhp) and the european h1n1 swine influenza virus, and the consequences for nutrient metabolism, with a focus on amino acids. two groups of specific pathogen- ... | 2014 | 25101681 |
| a comparative pathological finding in pigs exposed to fumonisin b1 and/or mycoplasma hyopneumoniae. | a more complicated pathology was observed in female pigs infected with mycoplasma hyopneumoniae, when the same were exposed to 20 ppm dietary levels of fumonisin b1 (fb1) starting 14 days before infection for a period of 42 days as was assessed by gross pathology and pathomorphological examinations or computed tomography, and also manifested by the strong deterioration of the pneumonic process in two pigs and the subsequent euthanizing of one pig. typical damages in fb1-fed pigs were a strong oe ... | 2016 | 25107460 |
| immune responses elicited by mycoplasma hyopneumoniae recombinant antigens and dna constructs with potential for use in vaccination against porcine enzootic pneumonia. | mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (pep) and causes major economic losses to the pig industry worldwide. commercially available vaccines provide only partial protection and are relatively expensive. in this study, we assessed the humoral and cellular immune responses to three recombinant antigens of m. hyopneumoniae. immune responses to selected domains of the p46, hsp70 and mnua antigens (p46102-253, hsp70212-601 and mnua182-378), delivered as recomb ... | 2014 | 25148775 |
| association of swine influenza h1n1 pandemic virus (siv-h1n1p) with porcine respiratory disease complex in sows from commercial pig farms in colombia. | porcine respiratory disease complex (prdc) is a serious health problem that mainly affects growing and finishing pigs. prdc is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus (prrsv), swine influenza virus (siv), mycoplasma hyopneumoniae (myh), actinobacillus pleuropneumoniae (app), pasteurella multocida and porcine circovirus 2 (pcv2). to characterize the specific role of swine influenza virus in prdc presentation in colombia, 1 ... | 2014 | 25160760 |
| in vitro activity of tylvalosin against spanish field strains of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae is involved in the porcine enzootic pneumonia and respiratory disease complex; therefore, the search for new treatment options that contribute to the control of this organism is relevant. the minimal inhibitory concentrations (mics) and minimal bactericidal concentrations of tylvalosin and 19 other antimicrobial agents against 20 spanish field isolates of m. hyopneumoniae were determined using the broth microdilution method, with the type strain (j) as a control strain. ... | 2014 | 25185108 |
| molecular epidemiology of mycoplasma hyopneumoniae from outbreaks of enzootic pneumonia in domestic pig and the role of wild boar. | mycoplasma hyopneumoniae is the major cause of enzootic pneumonia (ep) in domestic pigs, a disease with low mortality but high morbidity, having a great economic impact for producers. in switzerland ep has been successfully eradicated, however, sporadic outbreaks are observed with no obvious source. besides the possibility of recurrent outbreaks due to persisting m. hyopneumoniae strains within the pig population, there is suspicion that wild boars might introduce m. hyopneumoniae into swine her ... | 2014 | 25236987 |
| innate and adaptive immune responses of snatch-farrowed porcine-colostrum-deprived pigs to mycoplasma hyopneumoniae vaccination. | the snatch-farrowed porcine-colostrum-deprived (sf-pcd) pig model, in which neonates are raised on commercially available bovine colostrum, is an alternative model for porcine infectious disease research. it is not known if sf-pcd pigs possess growth performance and immunity comparable to conventional, farm-raised pigs. the current experiment compared growth performance and immune responses of sf-pcd pigs to their farm-raised siblings following mycoplasma hyopneumoniae (mhyo) vaccination. twelve ... | 2014 | 25239088 |
| antibody responses of swine following infection with mycoplasma hyopneumoniae, m. hyorhinis, m. hyosynoviae and m. flocculare. | several mycoplasma species possessing a range of virulence have been described in swine. the most commonly described are mycoplasma hyopneumoniae, mycoplasma hyorhinis, mycoplasma hyosynoviae, and mycoplasma flocculare. they are ubiquitious in many pig producing areas of the world, and except for m. hyopneumoniae, commercial antibody-based assays are lacking for most of these. antibody cross-reactivity among these four mycoplasma species is not well characterized. recently, the use of pen-based ... | 2014 | 25240775 |
| proteolytic processing of the cilium adhesin mhj_0194 (p123j ) in mycoplasma hyopneumoniae generates a functionally diverse array of cleavage fragments that bind multiple host molecules. | mycoplasma hyopneumoniae, the aetiological agent of porcine enzootic pneumonia, regulates the presentation of proteins on its cell surface via endoproteolysis, including those of the cilial adhesin p123 (mhj_0194). these proteolytic cleavage events create functional adhesins that bind to proteoglycans and glycoproteins on the surface of ciliated and non-ciliated epithelial cells and to the circulatory host molecule plasminogen. two dominant cleavage events of the p123 preprotein have been previo ... | 2015 | 25293691 |
| unravelling the transcriptome profile of the swine respiratory tract mycoplasmas. | the swine respiratory ciliary epithelium is mainly colonized by mycoplasma hyopneumoniae, mycoplasma flocculare and mycoplasma hyorhinis. while colonization by m. flocculare is virtually asymptomatic, m. hyopneumoniae and m. hyorhinis infections may cause respiratory disease. information regarding transcript structure and gene abundance provides valuable insight into gene function and regulation, which has not yet been analyzed on a genome-wide scale in these mycoplasma species. in this study, w ... | 2014 | 25333523 |
| severity of bovine tuberculosis is associated with co-infection with common pathogens in wild boar. | co-infections with parasites or viruses drive tuberculosis dynamics in humans, but little is known about their effects in other non-human hosts. this work aims to investigate the relationship between mycobacterium bovis infection and other pathogens in wild boar (sus scrofa), a recognized reservoir of bovine tuberculosis (btb) in mediterranean ecosystems. for this purpose, it has been assessed whether contacts with common concomitant pathogens are associated with the development of severe btb le ... | 2014 | 25350002 |
| survey of surface proteins from the pathogenic mycoplasma hyopneumoniae strain 7448 using a biotin cell surface labeling approach. | the characterization of the repertoire of proteins exposed on the cell surface by mycoplasma hyopneumoniae (m. hyopneumoniae), the etiological agent of enzootic pneumonia in pigs, is critical to understand physiological processes associated with bacterial infection capacity, survival and pathogenesis. previous in silico studies predicted that about a third of the genes in the m. hyopneumoniae genome code for surface proteins, but so far, just a few of them have experimental confirmation of their ... | 2014 | 25386928 |
| transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae. | porcine respiratory disease complex (prdc) is of great concern economically, for swine producers worldwide. co-infections with porcine reproductive and respiratory syndrome virus (prrsv) and mycoplasma hyopneumoniae (mhp) are considered the major causative agents of prdc, and responsible for mass mortality in pigs. nevertheless, the molecular mechanisms underlying the host factors involved in pathogenesis and persistent infection have not been clearly established because of a lack of information ... | 2015 | 25445346 |
| mycoplasma hyopneumoniae-derived lipid-associated membrane proteins induce inflammation and apoptosis in porcine peripheral blood mononuclear cells in vitro. | mycoplasma hyopneumoniae is the causative agent of swine enzootic pneumonia (ep), a disease that causes considerable economic losss in swine industry. lipid-associated membrane proteins (lamps) of mycoplasma play important roles in causing mycoplasma diseases. the present study explores the pathogenic mechanisms of m. hyopneumoniae lamps by elucidating their role in modulating the inflammation, apoptosis, and relevant signaling pathways of peripheral blood mononuclear cells (pbmcs) of pig. lamp ... | 2015 | 25481242 |
| genotype distribution of mycoplasma hyopneumoniae in swine herds from different geographical regions. | genetic heterogeneity of mycoplasma hyopneumoniae in pigs has been reported, however there has been limited reproducibility on the molecular methods employed so far. the aim of this study was to modify and standardize a high-resolution multiple locus variable number tandem repeat analysis (mlva), to investigate the genetic variability of m. hyopneumoniae circulating in the united states of america (usa), brazil, mexico and spain. the mlva was standardized on the basis of the number of tandem rep ... | 2015 | 25497236 |
| evaluation of adjuvant effects of fucoidan for improving vaccine efficacy. | fucoidan is a sulfated polysaccharide derived from brown seaweed, including fucus vesiculosus. this compound is known to have immunostimulatory effects on various types of immune cells including macrophages and dendritic cells. a recent study described the application of fucoidan as a vaccine adjuvant. vaccination is regarded as the most efficient prophylactic method for preventing harmful or epidemic diseases. to increase vaccine efficacy, effective adjuvants are needed. in the present study, w ... | 2015 | 25549218 |
| mhj_0461 is a multifunctional leucine aminopeptidase on the surface of mycoplasma hyopneumoniae. | aminopeptidases are part of the arsenal of virulence factors produced by bacterial pathogens that inactivate host immune peptides. mycoplasma hyopneumoniae is a genome-reduced pathogen of swine that lacks the genetic repertoire to synthesize amino acids and relies on the host for availability of amino acids for growth. m. hyopneumoniae recruits plasmin(ogen) onto its cell surface via the p97 and p102 adhesins and the glutamyl aminopeptidase mhj_0125. plasmin plays an important role in regulating ... | 2015 | 25589579 |
| erysipelothrix rhusiopathiae and mycoplasma hyopneumoniae: the sensitivities of enzyme-linked immunosorbent assays for detecting vaccinated sows of unknown disease status using serum and colostrum, and the correlation of the results for sow serum, colostrum, and piglet serum. | due to relatively high concentrations of immunoglobulins, colostrum has the potential to improve the sensitivity of diagnostic tests for diseases in pigs when compared with serum. it is possible that colostrum could improve the sensitivity of the antibody enzyme-linked immunosorbent assay (elisa) compared with serum. colostrum is also essential for piglets, providing protection against infections in the first few weeks and months of life. the sensitivity of 2 commercially available elisas, one f ... | 2015 | 25613041 |
| comparison of immune responses to intranasal and intrapulmonary vaccinations with the attenuated mycoplasma hyopneumoniae 168 strain in pigs. | the aim of this study was to evaluate the immune responses to intranasal and intrapulmonary vaccinations with the attenuated mycoplasma hyopneumoniae (mhp) 168 strain in the local respiratory tract in pigs. twenty-four pigs were randomly divided into 4 groups: an intranasal immunization group, an intrapulmonary immunization group, an intramuscular immunization group and a control group. the levels of local respiratory tract cellular and humoral immune responses were investigated. the levels of i ... | 2015 | 25649413 |
| exposure of feral swine (sus scrofa) in the united states to selected pathogens. | feral swine (sus scrofa) are widely distributed in the united states. in 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the us to evaluate exposure to important swine endemic pathogens. antibodies against porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) were found in 2.5% and 25.3% of tested sera, respectively. positive serological reactions against mycoplasma hyopneumoniae and actinobacillus p ... | 0 | 25673913 |
| investigating the role of free-ranging wild boar (sus scrofa) in the re-emergence of enzootic pneumonia in domestic pig herds: a pathological, prevalence and risk-factor study. | enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae has a significant economic impact on domestic pig production. a control program carried out from 1999 to 2003 successfully reduced disease occurrence in domestic pigs in switzerland, but recurrent outbreaks suggested a potential role of free-ranging wild boar (sus scrofa) as a source of re-infection. since little is known on the epidemiology of ep in wild boar populations, our aims were: (1) to estimate the prevalence of m. hyopneumoniae ... | 2015 | 25747151 |
| complete genome sequence of mycoplasma flocculare strain ms42t (atcc 27399t). | mycoplasma flocculare is a commensal or low-virulence pathogen of swine. the complete 778,866-bp genome sequence of m. flocculare strain ms42(t) has been determined, enabling further comparison to genomes of the closely related pathogen mycoplasma hyopneumoniae. the absence of the p97 and glpd genes may contribute to the attenuated virulence of m. flocculare. | 2015 | 25767245 |
| exposure of extensively farmed wild boars (sus scrofa scrofa) to selected pig pathogens in greece. | increased density and distribution of wild boar populations are likely to promote interactions and transmission of certain pathogens, not only among wild boar but also from wild boar to livestock or humans and vice versa. | 2015 | 25774434 |
| intrinsic terminators in mycoplasma hyopneumoniae transcription. | mycoplasma hyopneumoniae, an important pathogen of swine, exhibits a low guanine and cytosine (gc) content genome. m. hyopneumoniae genome is organised in long transcriptional units and promoter sequences have been mapped upstream of all transcription units. these analysis provided insights into the gene organisation and transcription initiation at the genome scale. however, the presence of transcriptional terminator sequences in the m. hyopneumoniae genome is poorly understood. | 2015 | 25886614 |
| characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and mycoplasma hyopneumoniae vaccines. | vaccines consisting of subunit or inactivated bacteria/virus and potent adjuvants are widely used to control and prevent infectious diseases. because inactivated and subunit antigens are often less antigenic than live microbes, a growing need exists for the development of new and improved vaccine adjuvants that can elicit rapid and long-lasting immunity. here we describe the development and characterization of a novel oil-in-water emulsion, ow-14. ow-14 contains low-cost plant-based emulsifiers ... | 2015 | 25936722 |
| a rapid and sensitive loop-mediated isothermal amplification procedure (lamp) for mycoplasma hyopneumoniae detection based on the p36 gene. | the aim of this study was to establish a method for sensitive and rapid diagnosis of mycoplasma hyopneumoniae in clinical specimens. to this effect, we employed three sets of primers specifically designed for amplification of nucleic acids under isothermal conditions. after optimization of reaction conditions, m. hyopneumoniae could be successfully detected at 63°c in 45 min through use of the loop-mediated isothermal amplification (lamp) assay. a positive reaction was identified visually as whi ... | 2015 | 25966242 |
| mycoplasma hyopneumoniae infections in peri-weaned and post-weaned pigs in belgium and the netherlands: prevalence and associations with climatic conditions. | mycoplasma hyopneumoniae (m. hyo) is an important pathogen in modern intensive pig farming in europe. the objectives of the present study were (1) to use the tracheobronchial swab (tbs) technique to obtain data on the distribution of m. hyo infections in recently weaned pigs in belgium and the netherlands, and (2) to look for associations between infection prevalence and specific climatic conditions. one hundred and seventy-six pig herds were randomly selected and 30 piglets sampled on each farm ... | 2015 | 25981930 |
| impact of particulate matter and ammonia on average daily weight gain, mortality and lung lesions in pigs. | the present study investigated the simultaneous influence of particulate matter (pm10) and ammonia (nh3) on performance, lung lesions and the presence of mycoplasma hyopneumoniae (m. hyopneumoniae) in finishing pigs. a pig herd experiencing clinical problems of m. hyopneumoniae infections was selected. in total, 1095 finishing pigs of two replicates in eight compartments each were investigated during the entire finishing period (fp). indoor pm10 and nh3 were measured at regular intervals during ... | 2015 | 26148844 |
| assessment of litter prevalence of mycoplasma hyopneumoniae in preweaned piglets utilizing an antemortem tracheobronchial mucus collection technique and a real-time polymerase chain reaction assay. | the swine industry currently lacks validated antemortem methods of detecting baseline herd prevalence of mycoplasma hyopneumoniae. the focus of our study was to evaluate alternative antemortem detection techniques and to determine baseline litter prevalence in preweaned pig populations utilizing the selected technique and a real-time polymerase chain reaction (qpcr) assay. preliminary data was analyzed on weaned piglets with evidence of respiratory disease (n = 32). five sample types (antemortem ... | 2015 | 26179099 |
| genetic association of the porcine c9 complement component with hemolytic complement activity. | the complement system is a part of the natural immune regulation mechanism against invading pathogens. complement activation from three different pathways (classical, lectin, and alternative) leads to the formation of c5-convertase, an enzyme for cleavage of c5 into c5a and c5b, followed by c6, c7, c8, and c9 in membrane attack complex. the c9 is the last complement component of the terminal lytic pathway, which plays an important role in lysis of the target cells depending on its self-polymeriz ... | 2015 | 26194222 |
| disease risks associated with free-ranging wild boar in saskatchewan. | this study investigated the disease status of saskatchewan's feral wild boar population. whole carcasses, tissue samples, and/or serum from 81 hunter-killed boars from saskatchewan were submitted to the canadian wildlife health cooperative (cwhc) between 2009 and 2014. serological tests were negative for prrs, h1n1, and h3n2 swine influenza, pcv-2, and tge/prcv in 22/22 boars and for toxoplasma gondii and mycoplasma hyopneumoniae in 20/20 boars. of 20 boars whose sera were tested 20 were positiv ... | 0 | 26246630 |
| immunogenic properties of landrace pigs selected for resistance to mycoplasma pneumonia of swine. | mycoplasma pneumonia of swine (mps) lung lesions and immunogenic properties were compared between a landrace line that was genetically selected for reduced incidence of pulmonary mps lesions, and a non-selected landrace line. the mps-selected landrace line showed significantly lower degrees of pulmonary mps lesions compared with the non-selected landrace line. when changes in immunity before and after vaccination were compared, the percentage of b cells in the peripheral blood of the mps-selecte ... | 2016 | 26260893 |
| swine influenza virus and association with the porcine respiratory disease complex in pig farms in southern brazil. | despite the putative endemic status of swine influenza a virus (swiav) infections, data on the occurrence of swine influenza outbreaks are scarce in brazil. the aim of this study was to detect and subtype swiavs from six outbreaks of porcine respiratory disease complex (prdc) in southern brazil. nasal swabs were collected from 66 piglets with signs of respiratory disease in six herds. lung tissue samples were collected from six necropsied animals. virus detection was performed by pcr screening a ... | 2016 | 26302164 |
| sonicated protein fractions of mycoplasma hyopneumoniae induce inflammatory responses and differential gene expression in a murine alveolar macrophage cell line. | mycoplasma hyopneumoniae is known to cause porcine enzootic pneumonia (ep), an important disease in swine production. the objective of this study was to examine the effects of sonicated protein fractions of m. hyopneumoniae on inflammatory response and gene expression in the murine alveolar macrophage mh-s cell line. the effects of sonicated protein fractions and intact m. hyopneumoniae on the gene expression of cytokines and inos were assessed using rt-pcr. the annealing control primer (acp)-ba ... | 2015 | 26370797 |
| immunogenic properties and mycoplasmal pneumonia of swine (mps) lung lesions in large white pigs selected for higher peripheral blood immune capacity. | immunogenic properties and mycoplasmal pneumonia of swine (mps) lung lesions were compared between the immunity-selected large white line and the non-selected large white line. the selected large white line showed a higher level of pulmonary mps lesions compared with the non-selected large white line. subsequent to vaccination, the percentage of natural killer cells and t cells (cd3(+) cd4(+) cd8(-) and cd3(+) cd4(-) cd8(+) t cells) were significantly increased in the non-selected line but remai ... | 2016 | 26388420 |
| increased mycoplasma hyopneumoniae disease prevalence in domestic hybrids among free-living wild boar. | wildlife immune genes are subject to natural selection exerted by pathogens. in contrast, domestic immune genes are largely protected from pathogen selection by veterinary care. introgression of domestic alleles into the wild could lead to increased disease susceptibility, but observations are scarce due to low introgression rates, low disease prevalence and reduced survival of domestic hybrids. here we report the first observation of a deleterious effect of domestic introgression on disease pre ... | 2015 | 26391376 |
| use of tylvalosin in the control of porcine enzootic pneumonia. | the purpose of this study was to investigate the efficacy of tylvalosin (aivlosin water soluble granules, eco animal health) in drinking water for control of mycoplasma hyopneumoniae (m hyo) on a farm with chronic enzootic pneumonia (ep) problems and high prevalence of mycoplasma-like lesions at slaughter. | 2015 | 26392896 |
| a serosurvey for selected pathogens in greek european wild boar. | serum samples, collected from 94 european wild boar (sus scrofa) during the hunting seasons 2006 -2010 from different regions of greece, were examined in order to estimate the role of these wildlife species as reservoir of pathogens important for livestock and/or public health. | 2015 | 26392908 |
| efficacy of combined vaccination against mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus in dually infected pigs. | porcine respiratory disease complex (prdc) is one of the main causes of economic losses for swine producers. this complex is due to a combination of different pathogens and their interactions. two major pathogens involved in prdc are mycoplasma hyopneumoniae (mhp) and porcine reproductive and respiratory syndrome virus (prrsv). the objectives of this study were (i) to develop an experimental model of dual mhp/prrsv infection in spf pigs with european strains of mhp and prrsv and (ii) to assess a ... | 2015 | 26422712 |
| apoptosis induced by lipid-associated membrane proteins from mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the mapk pathway. | lipid-associated membrane proteins (lamps) are important in the pathogenicity of the mycoplasma genus of bacteria. we investigated whether mycoplasma hyopneumoniae lamps have pathogenic potential by inducing apoptosis in a st. jude porcine lung epithelial cell line (sjpl). lamps from a pathogenic strain of m. hyopneumoniae (strain 232) were used in the research. our investigation made use of diamidino-phenylindole (dapi) and acridine orange/ethidium bromide (ao/eb) staining, terminal dexynucleot ... | 2015 | 26436384 |
| mlva typing of mycoplasma hyopneumoniae bacterins and field strains. | because of the lack of information about both the genetic characteristics of mycoplasma hyopneumoniae commercial vaccines and their relationship with field strains, the authors attempted to identify genetic subtypes of some m hyopneumoniae bacterins, and to compare them with m. hyopneumoniae field strains. six commercial m hyopneumoniae bacterins and 28 bronchoalveolar lavages from pigs at slaughter from three herds were analysed by multiple-locus variable number tandem repeat analysis (mlva) on ... | 2015 | 26495127 |
| efficacy of a new bivalent vaccine of porcine circovirus type 2 and mycoplasma hyopneumoniae (fostera™ pcv mh) under experimental conditions. | the objective of this study was to evaluate the efficacy of a new bivalent vaccine (fostera™ pcv mh, zoetis) of porcine circovirus type 2 (pcv2) and mycoplasma hyopneumoniae in growing pigs under experimental conditions. a total of 80 pigs were randomly divided into 8 groups (10 pigs per group). the pigs were administered the bivalent vaccine intramuscularly as a 2.0 ml dose at 21 days of age based on the manufacturer's instructions. three weeks after vaccination, the pigs were inoculated with e ... | 2016 | 26626212 |
| association between pneumocystis spp. and co-infections with bordetella bronchiseptica, mycoplasma hyopneumoniae and pasteurella multocida in austrian pigs with pneumonia. | in this retrospective study, 218 pig lung tissue samples were analyzed to examine a possible association between pneumocystis spp. using in situ hybridization, bordetella bronchiseptica (b.b.) using immunohistochemistry (ihc), mycoplasma hyopneumoniae (m.h.) by quantitative pcr, and pasteurella multocida (p.m.; ihc). compared to the bacterial agents (b.b., 5%; m.h., 30%; p.m., 23%), pneumocystis occurred with a higher prevalence (51%). co-infections with two or three pathogens were present in 28 ... | 2016 | 26654847 |
| multiplex pcr and microarray for detection of swine respiratory pathogens. | porcine respiratory disease complex (prdc) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. no simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with prdc. furthermore, the detection of most of the bacterial pathogens implicated in prdc currently requires time-consuming culture-based methods that can take several days to obtain results. in this study, ... | 2015 | 26662640 |
| molecular characterisation of mycoplasma hyorhinis isolated from pigs using pulsed-field gel electrophoresis and 16s rrna sequencing. | economic loss in pig breeding is common due to respiratory disorders, and mycoplasma hyopneumoniae and mycoplasma hyorhinis, namely, are the most common infectious agents. the aim of this study is to recover these mollicutes and detect their genotypic variations by pulsed-field gel electrophoresis (pfge) and sequencing the 16 s rrna gene. one hundred and twenty-six swabs from tonsil and nasal mucus of pigs with respiratory disorders were analysed. a total of 78 lungs were sampled, as well as two ... | 2015 | 26688737 |
| a new single-dose bivalent vaccine of porcine circovirus type 2 and mycoplasma hyopneumoniae elicits protective immunity and improves growth performance under field conditions. | the efficacy of the new single-dose bivalent vaccine of porcine circovirus type 2 (pcv2) and mycoplasma hyopneumoniae was evaluated under field conditions for registration as recommended by the republic of korea's animal, plant & fisheries quarantine & inspection agency. three farms were selected based on their history of co-infection with pcv2 and m. hyopneumoniae. on each farm, a total of 80 3-week-old pigs were randomly allocated to one of two treatment groups: (i) vaccinated (n=40) and (ii) ... | 2016 | 26711046 |
| evaluating the risk of pathogen transmission from wild animals to domestic pigs in australia. | wild animals contribute to endemic infection in livestock as well as the introduction, reintroduction and maintenance of pathogens. the source of introduction of endemic diseases to a piggery is often unknown and the extent of wildlife contribution to such local spread is largely unexplored. the aim of the current study was to quantitatively assess the probability of domestic pigs being exposed to different pathogens from wild animals commonly found around commercial piggeries in australia. spec ... | 2016 | 26711303 |
| assessment of mycoplasma hyopneumoniae-induced pneumonia using different lung lesion scoring systems: a comparative review. | mycoplasma hyopneumoniae is the primary aetiological agent of swine enzootic pneumonia (ep) and one of the major contributors to the porcine respiratory disease complex (prdc). gross lung lesions in pigs affected by ep consist of cranioventral pulmonary consolidation (cvpc), usually distributed bilaterally in the apical, intermediate, accessory and cranial parts of the diaphragmatic lobes. several lung scoring methods are currently in place for the evaluation of cvpc. the aims of this study were ... | 2017 | 26774274 |
| mycoplasma hyopneumoniae management in gilts. | 2016 | 26823313 | |
| increased fucosyl glycoconjugate by mycoplasma hyopneumoniae enhances adherences of pasteurella multocida type a in the ciliated epithelial cells of the respiratory tract. | the objective of this study was to elucidate the pathogenic mechanisms of how mycoplasma hyopneumoniae enhances secondary pasteurella multocida type a infection which leads to porcine enzootic pneumonia in infected pigs. sixteen pigs were experimentally infected with m. hyopneumoniae and then euthanized at 7, 14, 21 and 28 days post inoculation. in situ hybridization for m. hyopneumoniae dna and ulex europaeus agglutinin-i (uea-i) lectin histochemistry for fucosyl glycoconjugate, was performed i ... | 2016 | 26841756 |
| a model to investigate the optimal seeder-to-naïve ratio for successful natural mycoplasma hyopneumoniae gilt exposure prior to entering the breeding herd. | due to the significance of mycoplasma hyopneumoniae as a swine respiratory pathogen, acclimation measures are taken into consideration when obtaining replacement gilts from negative sources to be introduced to endemically infected herds. the aim of this study was to evaluate the optimum seeder-to-naïve gilt ratio in a 4-week period for successful natural exposure to m. hyopneumoniae. sixty gilts were divided in two groups, 21 2-week old seeder gilts were inoculated with m. hyopneumoniae, and 39 ... | 2016 | 26854344 |
| post-translational processing targets functionally diverse proteins in mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae is a genome-reduced, cell wall-less, bacterial pathogen with a predicted coding capacity of less than 700 proteins and is one of the smallest self-replicating pathogens. the cell surface of m. hyopneumoniae is extensively modified by processing events that target the p97 and p102 adhesin families. here, we present analyses of the proteome of m. hyopneumoniae-type strain j using protein-centric approaches (one- and two-dimensional gelc-ms/ms) that enabled us to focus on g ... | 2016 | 26865024 |
| relationship between expression of interleukin-5 and interleukin-13 by epithelial cells and bronchiolar changes in pigs infected with mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae (mh) is a bacterium that specifically infects the surface of bronchi and bronchioles of pigs without invading the host cells, and it is considered to be the primary agent of porcine enzootic pneumonia (pen). the present study investigates the morphological and immunohistological changes induced in bronchiolar epithelium by mh infection. lungs from 20 pigs with naturally occurring mh pneumonia were compared with those from 10 uninfected controls. bronchiolar epithelial he ... | 2017 | 26922858 |
| mycoplasma hyopneumoniae genetic variability within a swine operation. | the objective of our study was to characterize the mycoplasma hyopneumoniae genetic diversity within a swine operation comingling weaned pigs. bronchial swabs and tracheal aspirates were collected from 3 nursery-to-finish farms. during the finishing production stages, samples were obtained from mortalities and from live coughing pigs in rooms where mortality was not observed. a total of 105 samples were examined by a m. hyopneumoniae real-time polymerase chain reaction and subjected to genetic t ... | 2016 | 26965239 |
| efficacy of mycoplasma hyopneumoniae vaccination before and at weaning against experimental challenge infection in pigs. | commercial bacterins are widely used at weaning to control mycoplasma hyopneumoniae infections in pigs. however, it is not known whether the efficacy of vaccinating against m. hyopneumoniae can be influenced by the weaning process when vaccination is applied at the day of weaning. the present study assessed the efficacy of a single m. hyopneumoniae vaccination (ingelvac mycoflex®) three days before weaning (v1) or at weaning (v2) against experimental challenge infection. four weeks after vaccina ... | 2016 | 27025924 |
| the effects of mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro pk-15 cells. | porcine circovirus type 2 (pcv2) is the causative agent of postweaning multisystemic wasting syndrome (pmws). mycoplasma hyopneumoniae (mhp) is a very well-known co-factor that potentially enhances pcv2 replication and thus the development of pmws. however, co-infection with mhp and pcv2 in vivo under different conditions can produce divergent clinical signs and lesions. in this study, pcv2 replication could be enhanced by subsequent co-inoculation with mhp (pcv2+mhp) in a time and dose dependen ... | 2016 | 27033909 |
| development of a blocking elisa for detection of mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein p65. | mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. a more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of immunization. we expressed the m. hyopneumoniae protein p65 in escherichia coli and produced a monoclonal antibody (mab) that bound specifically to recombinant p65. using this mab, a blocking enzyme linked immunosorbent assay (elisa) was developed. t ... | 2016 | 27075114 |
| development of mycoplasma hyopneumoniae recombinant vaccines. | mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (ep), a disease that affects swine production worldwide. vaccination is the most cost-effective strategy for the control and prevention of the disease. research using genome-based approach has the potential to elucidate the biology and pathogenesis of m. hyopneumoniae and contribute to the development of more effective vaccines. here, we describe the protocol for developing m. hyopneumoniae recombinant vaccines using r ... | 2016 | 27076288 |
| comparison of 2 commercial single-dose mycoplasma hyopneumoniae vaccines and porcine reproductive and respiratory syndrome virus (prrsv) vaccines on pigs dually infected with m. hyopneumoniae and prrsv. | the objective of this study was to compare the efficacy of 2 different commercial mycoplasma hyopneumoniae vaccines and porcine reproductive and respiratory syndrome virus (prrsv) vaccines in regard to growth performance, microbiological and immunological analyses, and pathological observation from wean to finish (175 d of age). pigs were administered m. hyopneumoniae and prrsv vaccines at 7 and 21 d of age, respectively, or both at 21 d old and then challenged with both m. hyopneumoniae and prr ... | 2016 | 27127338 |
| dynamics of mycoplasma hyopneumoniae seroconversion and infection in pigs in the three main production systems. | in this study, we investigated the dynamics of mycoplasma hyopneumoniae infections in 66 pig farms, with different production systems (one-, two-, and three-site systems), and considered different risk factors. serological assay was used to detect serum antibodies against m. hyopneumoniae and real time polymerase chain reaction (rt-pcr) was performed to detect m. hyopneumoniae dna in tracheobronchial swabs. results demonstrated that m. hyopneumoniae infection status was predominantly influenced ... | 2016 | 27142053 |
| induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes. | the purpose of this study was to assess the effect of three different inoculation routes into mycoplasmal pneumonia (mp) in pigs challenged with mycoplasma hyopneumoniae (m. hyopneumoniae). thirty six-week-old m. hyopneumoniae seronegative piglets were randomly assigned to four groups: three challenged groups with experimentally inoculated pigs by either the endotracheal (et; n = 8), intranasal (in; n = 8) or aerosol (ae; n = 8) routes and one uninfected group (control; n = 6). blood samples wer ... | 2016 | 27160189 |
| a replicating plasmid-based vector for gfp expression in mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae (m. hyopneumoniae) causes porcine enzootic pneumonia (pep) that significantly affects the pig industry worldwide. despite the availability of the whole genome sequence, studies on the pathogenesis of this organism have been limited due to the lack of a genetic manipulation system. therefore, the aim of the current study was to generate a general gfp reporter vector based on a replicating plasmid. here, we describe the feasibility of gfp reporter expression in m. hyopneum ... | 2016 | 27173288 |
| insights on the virulence of swine respiratory tract mycoplasmas through genome-scale metabolic modeling. | the respiratory tract of swine is colonized by several bacteria among which are three mycoplasma species: mycoplasma flocculare, mycoplasma hyopneumoniae and mycoplasma hyorhinis. while colonization by m. flocculare is virtually asymptomatic, m. hyopneumoniae is the causative agent of enzootic pneumonia and m. hyorhinis is present in cases of pneumonia, polyserositis and arthritis. the genomic resemblance among these three mycoplasma species combined with their different levels of pathogenicity ... | 2016 | 27178561 |
| in vitro protective efficacy of lithium chloride against mycoplasma hyopneumoniae infection. | mycoplasma hyopneumoniae (m. hyopneumoniae) infection affects the swine industry. lithium chloride (licl), is a drug used to treat bipolar disorder and has also shown activity against bacterial and viral infections. herein, we evaluated the antibacterial activity of licl on pk-15 cells infected with m. hyopneumoniae. incubation of licl (40mm) with cells for 24h, did not significantly affect the cell viability. the qrt-pcr showed ~80% reduction in m. hyopneumoniae genome when licl added post-infe ... | 2016 | 27234543 |
| porcine respiratory disease complex: interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, and mycoplasma hyopneumoniae. | porcine respiratory disease is a multifactorial and complex disease caused by a combination of infectious pathogens, environmental stressors, differences in production systems, and various management practices; hence the name porcine respiratory disease complex (prdc) is used. porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), and mycoplasma hyopneumoniae are considered to be the most important pathogens that cause prdc. although interactions among the ... | 2016 | 27256017 |
| mycoplasma hyopneumoniae and mycoplasma flocculare differential domains from orthologous surface proteins induce distinct cellular immune responses in mice. | mycoplasma hyopneumoniae and mycoplasma flocculare are two genetically close species found in the swine respiratory tract. despite their similarities, while m. hyopneumoniae is the causative agent of porcine enzootic pneumonia, m. flocculare is a commensal bacterium. genomic and transcriptional comparative analyses so far failed to explain the difference in pathogenicity between these two species. we then hypothesized that such difference might be, at least in part, explained by amino acid seque ... | 2016 | 27283856 |
| genetic variability and limited clonality of mycoplasma hyorhinis in pig herds. | mycoplasma hyorhinis is a common inhabitant of the upper respiratory tract and tonsils of pigs. its role as a possible pathogen remains controversial. in order to gain more insight into the epidemiology and population structure of m. hyorhinis we genetically characterized 60 isolates by multi locus sequence typing (mlst). the m. hyorhinis strains originated from swiss and german pig herds with knowledge on the clinical background. the mlst scheme of tocqueville et al. (j. clin. microbiol. 2014) ... | 2016 | 27374901 |
| cyp1a1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (pam) cell lines caused by mycoplasma hyponeumoniae. | mycoplasmal pneumonia is a lung infection disease caused by mycoplasma hyopneumoniae in swine. we previously reported that cytochrome p450 1a1 (cyp1a1) expression was significantly downregulated in pigs infected with m. hyopneumoniae compared to the healthy controls. in this study, pulmonary alveolar macrophage (pam) cell lines with cyp1a1 overexpression or sirna-mediated cyp1a1 silencing were used to explore the biological function and regulatory mechanism of cyp1a1 gene expression changed on t ... | 2016 | 27377584 |
| retrospective analysis of bacterial and viral co-infections in pneumocystis spp. positive lung samples of austrian pigs with pneumonia. | aim of this study was the retrospective investigation of viral (porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), torque teno sus virus type 1 and 2 (ttsuv1, ttsuv2)) and bacterial (bordetella bronchiseptica (b. b.), mycoplasma hyopneumoniae (m. h.), and pasteurella multocida (p. m.)) co-infections in 110 pneumocystis spp. positive lung samples of austrian pigs with pneumonia. fifty-one % were positive for pcv2, 7% for prrsv, 22% for ttsuv1, 48% for t ... | 2016 | 27428002 |
| mycoplasma hyorhinis is a potential pathogen of porcine respiratory disease complex that aggravates pneumonia caused by porcine reproductive and respiratory syndrome virus. | the porcine respiratory disease complex (prdc) caused by numerous bacterial and viral agents has a great impact on pig industry worldwide. although mycoplasma hyorhinis (mhr) has been frequently isolated from lung lesions from pigs with prdc, the pathological importance of mhr may have been underestimated. in this study, 383 serum samples obtained from seven herds with a history of prdc were tested for specific antibodies to mhr, mycoplasma hyopneumoniae (mhp), and porcine reproductive and respi ... | 2016 | 27436444 |
| mycoplasma hyopneumoniae does not affect the interferon-related anti-viral response but predisposes the pig to a higher level of inflammation following swine influenza virus infection. | in pigs, influenza a viruses and mycoplasma hyopneumoniae (mhp) are major contributors to the porcine respiratory disease complex. pre-infection with mhp was previously shown experimentally to exacerbate the clinical outcomes of h1n1 infection during the first week after virus inoculation. in order to better understand the interactions between these pathogens, we aimed to assess very early responses (at 5, 24 and 48 h) after h1n1 infection in pigs pre-infected or not with mhp. clinical signs and ... | 2016 | 27498789 |
| factors associated with herd-level prrsv infection and age-time to seroconversion in farrow-to-finish herds. | factors associated with porcine reproductive and respiratory syndrome virus (prrsv) infection were investigated in 109 herds. serums from four batches of pigs (4, 10, 16 and 22 weeks, 15 pigs/batch) were tested by elisa for prrsv antibodies. infection by mycoplasma hyopneumoniae (mhp), actinobacillus pleuropneumoniae, h1n1 and h1n2 swine influenza a viruses (swiav) and pcv2 were detected by specific serological or pcr tests. data related to herd characteristics, biosecurity, management housing a ... | 2016 | 27527759 |
| cross reactivity among the swine mycoplasmas as identified by protein microarray. | mycoplasmas are cell wall-less bacteria that infect a variety of animals in a species-specific manner. in swine, mycoplasma hyopneumoniae is the most virulent and presents the most disease and economic problems to the swine industry. serological assays are commonly used to assess colonization and disease, but antigenic cross-reactivity between m. hyopneumoniae and other mycoplasma species, most notably mycoplasma hyorhinis, mycoplasma hyosynoviae and mycoplasma flocculare, is a concern. the exte ... | 2016 | 27527784 |
| a survey of porcine respiratory disease complex (prdc) associated pathogens among commercial pig farms of korea via oral fluid method. | oral fluid analysis for monitoring herd is an interest in commercial pig farms in korea. the aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (prdc) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. the samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (rt-pcr) or standard pcr depending on microorganisms. the pathogens ... | 2016 | 27586468 |
| in vitro antimicrobial activities of animal-used quinoxaline 1,4-di-n-oxides against mycobacteria, mycoplasma and fungi. | the quinoxaline 1,4-di-n-oxides (qdnos) were known as potent antibacterial agents. for the purpose of evaluating the bioactivity of existing animal-used qdnos drugs against representative pathogenic microorganism, the representative drugs of quinoxalines including cyadox, mequindox, quinocetone and their metabolites were submitted to the in vitro evaluation for antituberculosis, antimycoplasma, antifungal and antiviral activities. | 2016 | 27600955 |
| bordetella bronchiseptica antigen enhances the production of mycoplasma hyopneumoniae antigen-specific immunoglobulin g in mice. | we previously demonstrated that bordetella bronchiseptica (b. bronchiseptica) antigen (ag) showed high immunostimulatory effects on mouse bone marrow cells (bms) while mycoplasma hyopneumoniae (m. hyopneumoniae) ag showed low. the focus of this study was if b. bronchiseptica ag may enhance m. hyopneumoniae ag-specific immune response, and also the host immune system can recognize both ags. mtt assay revealed that each or both ags did not significantly change the cell metabolic activity of bms. f ... | 2016 | 27659847 |
| serological patterns of actinobacillus pleuropneumoniae, mycoplasma hyopneumoniae, pasteurella multocida and streptococcus suis in pig herds affected by pleuritis. | respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. these bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. the aim of this study was to evaluate the presence of a. pleuropneumoniae and m. hyopneumoniae, ... | 2016 | 27716292 |
| low temperature and dust favour in vitro survival of mycoplasma hyopneumoniae: time to revisit indirect transmission in pig housing. | porcine enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae adversely affects pig welfare and is associated with major economic losses in the pig industry worldwide. transmission is predominantly by direct contact, but the role of indirect transmission remains poorly understood. this study examined survival of six m. hyopneumoniae isolates dried onto five different surfaces encountered in pig units and exposed to temperatures of 4, 25 and 37°c for up to 12 days. survival of the organisms ... | 2017 | 27759918 |
| selective medium for culture of mycoplasma hyopneumoniae. | the fastidious porcine respiratory pathogen mycoplasma hyopneumoniae has proven difficult to culture since it was first isolated in 1965. a reliable solid medium has been particularly challenging. moreover, clinical and pathological samples often contain the fast-growing m. hyorhinis which contaminates and overgrows m. hyopneumoniae in primary culture. the aim of this study was to optimise the culture medium for recovery of m. hyopneumoniae and to devise a medium for selection of m. hyopneumonia ... | 2016 | 27771062 |
| mycoplasma non-coding rna: identification of small rnas and targets. | bacterial non-coding rnas act by base-pairing as regulatory elements in crucial biological processes. we performed the identification of trans-encoded small rnas (srna) from the genomes of mycoplama hyopneumoniae, mycoplasma flocculare and mycoplasma hyorhinis, which are mycoplasma species that have been identified in the porcine respiratory system. | 2016 | 27801290 |
| evolution and function of the mycoplasma hyopneumoniae peroxiredoxin, a 2-cys-like enzyme with a single cys residue. | the minimal genome of the mollicute mycoplasma hyopneumoniae, the etiological agent of porcine enzootic pneumonia, encodes a limited repertoire of antioxidant enzymes that include a single and atypical peroxiredoxin (mhprx), whose evolution and function were studied here. mhprx has only one catalytic cysteine, in contrast with some of its possible ancestors (2-cys peroxiredoxins), which have two. although it is more similar to 2-cys orthologs, mhprx can still function with a single peroxidatic c ... | 2017 | 27858147 |
| secretomes of mycoplasma hyopneumoniae and mycoplasma flocculare reveal differences associated to pathogenesis. | mycoplasma hyopneumoniae and mycoplasma flocculare cohabit the porcine respiratory tract. however, m. hyopneumoniae causes the porcine enzootic pneumonia, while m. flocculare is a commensal bacterium. comparative analyses demonstrated high similarity between these species, which includes the sharing of all predicted virulence factors. nevertheless, studies related to soluble secretomes of mycoplasmas were little known, although they are important for bacterial-host interactions. the aim of this ... | 2017 | 28003119 |
| repetitive elements in mycoplasma hyopneumoniae transcriptional regulation. | transcriptional regulation, a multiple-step process, is still poorly understood in the important pig pathogen mycoplasma hyopneumoniae. basic motifs like promoters and terminators have already been described, but no other cis-regulatory elements have been found. dna repeat sequences have been shown to be an interesting potential source of cis-regulatory elements. in this work, a genome-wide search for tandem and palindromic repetitive elements was performed in the intergenic regions of all codin ... | 2016 | 28005945 |
| effects of mycoplasma hyopneumoniae on porcine nasal cavity dendritic cells. | mycoplasma hyopneumoniae (mhp) is the primary etiological agent responsible for swine enzootic pneumonia (ep), a disease that cause tremendous economic losses all over the swine industry. dendritic cells (dcs), the most effective antigen-presenting cells, are widely distributed beneath respiratory epithelium. dcs uptake and present antigens to t cells, to initiate protective immune responses or generate immune-mediated pathology in different infections. in this study, we investigated the changes ... | 2017 | 28061998 |
| impact of diversity of mycoplasma hyopneumoniae strains on lung lesions in slaughter pigs. | the importance of diversity of mycoplasma hyopneumoniae (m. hyopneumoniae) strains is not yet fully known. this study investigated the genetic diversity of m. hyopneumoniae strains in ten pig herds, and assessed associations between the presence of different strains of m. hyopneumoniae and lung lesions at slaughter. within each herd, three batches of slaughter pigs were investigated. at slaughter, from each batch, 20 post mortem bronchoalveolar lavage fluid samples were collected for multiple lo ... | 2017 | 28095890 |
| no clear effect of initiating vaccination against common endemic infections on the amounts of prescribed antimicrobials for danish weaner and finishing pigs during 2007-2013. | it is often stated that vaccines may help reduce antimicrobial use in swine production. however, limited evidence is available outside clinical trials. we studied the change in amounts of antimicrobials prescribed for weaners and finishers in herds following initiation of vaccination against five common endemic infections: mycoplasma hyopneumoniae, actinobacillus pleuropneumoniae, porcine circovirus type ii, porcine reproductive and respiratory syndrome virus, and lawsonia intracellularis. compa ... | 2016 | 28138438 |
| assessment of the adjuvant activity of mesoporous silica nanoparticles in recombinant mycoplasma hyopneumoniae antigen vaccines. | the adjuvant potential of two mesoporous silica nanoparticles (msns), sba-15 and sba-16, was assessed in combination with a recombinant hsp70 surface polypeptide domain from mycoplasma hyopneumoniae, the etiological agent of porcine enzootic pneumonia (pep). the recombinant antigen (hsp70212-600), previously shown as immunogenic in formulation with classic adjuvants, was used to immunize balb/c mice in combination with sba-15 or sba-16 msns, and the effects obtained with these formulations were ... | 2017 | 28194450 |
| a novel chimeric protein composed of recombinant mycoplasma hyopneumoniae antigens as a vaccine candidate evaluated in mice. | enzootic pneumonia (ep) is caused by the mycoplasma hyopneumoniae pathogenic bacteria, and it represents a significant respiratory disease that is responsible for major economic losses within the pig industry throughout the world. the bacterins that are currently commercially available have been proven to offer only partial protection against m. hyopneumoniae, and the development of more efficient vaccines is required. several recombinant antigens have been evaluated via different immunization s ... | 2017 | 28284602 |
| pro-apoptotic effect of a mycoplasma hyopneumoniae putative type i signal peptidase on pk(15) swine cells. | mycoplasma hyopneumoniae is an economically significant swine pathogen that causes porcine enzootic pneumonia (pep). important processes for swine infection by m. hyopneumoniae depend on cell surface proteins, many of which are secreted by secretion pathways not completely elucidated so far. a putative type i signal peptidase (spase i), a possible component of a putative sec-dependent pathway, was annotated as a product of the sips gene in the pathogenic m. hyopneumoniae 7448 genome. this m. hyo ... | 2017 | 28284605 |
| serum acute phase response induced by different vaccination protocols against circovirus type 2 and mycoplasma hyopneumoniae in piglets. | the purpose of this study was to compare the acute phase reaction (apr) induced by different vaccination protocols used against porcine circovirus (pcv) type-2 and mycoplasma hyopneumoniae (m.hyo), studying two acute phase proteins (apps) and changes in rectal temperature (rt). in addition, the possible influence of the time of vaccination and breed were analysed. in the first experiment, 40 commercial crossbred piglets were vaccinated, on the day of weaning, with flexcombo® (group a, n=20) or p ... | 2017 | 28324811 |
| potential use of local and systemic humoral immune response parameters to forecast mycoplasma hyopneumoniae associated lung lesions. | immunopathological events are key for the development of enzootic pneumonia (ep), which is macroscopically observed as cranioventral pulmonary consolidation (cvpc). this study aimed to investigate the putative association between the humoral immune response against mycoplasma hyopneumoniae (m. hyopneumoniae) and prevalence and extension of cvpc in 1) experimentally infected pigs, 2) slaughtered pigs and 3) sequentially necropsied pigs in a longitudinal study. cvpc was scored by means of the euro ... | 2017 | 28380065 |
| a randomized controlled study on the efficacy of a novel combination vaccine against enzootic pneumonia (mycoplasma hyopneumoniae) and porcine circovirus type 2 (pcv2) in the presence of strong maternally derived pcv2 immunity in pigs. | mycoplasma hyopneumoniae (m. hyo) and porcine circovirus type 2 (pcv2) are major pathogens that cause significant health problems in swine worldwide. maternal derived immunity (mdi) has been suggested as a significant immediate defence factor for newborn piglets and may interfere with piglet's vaccination-induced immunity. the study aimed to assess the efficacy of a novel combination vaccine (consisting of pcv2 subunits and inactivated m. hyo strain j), against pcv2 and m. hyo natural infection ... | 2017 | 28388953 |
| field efficacy study of a novel ready-to-use vaccine against mycoplasma hyopneumoniae and porcine circovirus type 2 in a greek farm. | the primary objective of this study was to assess the efficacy, under field conditions, of a novel ready-to use mycoplasma hyopneumoniae (m hyo) and porcine circovirus type 2 (pcv2) combination vaccine given to piglets as one vaccination (1-shot) at 3 weeks of age. the study was carried out according to a controlled, randomised, and blinded design in a greek pig herd with clinical m. hyo and subclinical pcv2 infection. moreover, based on serology at the time of vaccination, the average pcv2 titr ... | 2015 | 28405421 |
| influence of spray dried porcine plasma in starter diets associated with a conventional vaccination program on wean to finish performance. | conventional vaccination programs using a single injection of a combined vaccine against porcine circovirus type 2 (pcv2) and mycoplasma hyopneumoniae (mhyo) can promote a strong immune response that reduces feed intake for 24 to 48 h post injection. often such vaccines are given around the time of weaning during a critical stress period in which feed intake is already compromised. spray dried porcine plasma (sdpp) is a protein source used in starter diets that increases post-weaning feed intake ... | 2016 | 28405430 |
| modelling the within-herd transmission of mycoplasma hyopneumoniae in closed pig herds. | a discrete time, stochastic, compartmental model simulating the spread of mycoplasma hyopneumoniae within a batch of industrially raised pigs was developed to understand infection dynamics and to assess the impact of a range of husbandry practices. a 'disease severity' index was calculated based on the ratio between the cumulative numbers of acutely and chronically diseased and infectious pigs per day in each age category, divided by the length of time that pigs spent in this age category. this ... | 2016 | 28405436 |
| comparison of the antimicrobial consumption in weaning pigs in danish sow herds with different vaccine purchase patterns during 2013. | there is growing concern about development of antimicrobial resistance due to use of antimicrobials (ams) in livestock production. identifying efficient alternatives, including vaccination, is a priority. the objective of this study was to compare the herd-level amount of ams prescribed for weaner pigs, between danish sow herds using varying combinations of vaccines against porcine circovirus type 2 (pcv2), mycoplasma hyopneumoniae (myc) and lawsonia intracellularis (law). it was hypothesised th ... | 2016 | 28405449 |