Publications

regression of a hamster melanoma with intralesional corynebacterium granulosum.	the present study was undertaken to compare the antitumour effects of intralesionally administered corynebacterium granulosum, corynebacterium parvum, bcg and saline on a hamster melanoma. forty-eight golden hamsters were inoculated subcutaneously with forthers melanotic melanoma no. 1. twenty-four animals bore palpable tumours which were excised. no non-excised group animal showed primary tumour regression. in the excised group, the tumours generally recurred at the excision site in the c. parv ...	1976	949459
treatment of rat fibrosarcoma by radiotherapy plus immune adjuvant.	combined radiotherapy and nonspecific adjuvant c. parvum or piromen treatment of rat tumors show improvement over radiotherapy alone. the most effective protocol, resulting in complete remission in 6 of 6 rats was obtained with c. parvum given i.p. in three doses 1 day prior to tumor x-irradiation of three doses of 1500 r each given on days 1, 4, and 8. animals receiving the same dose schedule without adjuvant had only partial regression of their tumor. without adjuvant, increasing the dose to 6 ...	1976	949523
radiation therapy and corynebacterium parvum in the treatment of murine tumors.	the relative effectiveness of local irradiation alone or combined with corynebacterium parvum (c parvum) treatment has been investigated employing four tumors: a mammary carcinoma (mca) (nonimmunogenic), a fibrosarcoma (moderately strongly immunogenic), and two squamous cell carcinomas (scc-2 being weakly and scc-4 being very weakly or nonimmunogenic). c parvum treatment was started when the isotransplanted tumor growing in the mouse leg was 5 mm in diameter and the local irradiation was adminis ...	1976	949676
differential quantitation of surface and subsurface bacteria of normal skin by the combined use of the cotton swab and the scrub methods.	by testing adjacent sites on the hypothenar eminence of the palm, enriched with bacteria by massaging the forehead, we found that the numbers of bacteria recovered from the skin surface by a wet cotton swab in 30 s were not significantly different from the numbers obtained by a brisk scrubbing with a blunted teflon policeman for 120 s. this was true of aerobes (gram-positive cocci) and anaerobes (propionibacteria). if the same site on the palm was swabbed two times for 15 s each time, 67 to 94% ...	1976	950377
vitamin b12 biosynthesis: in vitro formation of cobinamide from cobyric acid and l-threonine.		1976	952513
proceedings: local corynebacterium parvum therapy in early breast cancer.		1976	953485
proceedings: the effect of corynebacterium parvum on a subcutaneous tumour.		1976	953487
intra-lesional injection of immunostimulants in bilateral rat tumors.	rats grafted with either two or six fragments of isogenic methylcholanthrene-induced transplantable fibrosarcomas were treated it1 with living bcg or killed c. parvum or a mixture of both immunostimulants. various tumor combination and therapeutic agent dosages were compared. when two fragments of the same tumor mcfifi2 (s) were grafted simultaneously, it treatment of one of these with 2 mg of bcg induced cure of both in 50% of the animals, but it injection of 2 x 10(9) c. parvum was completely ...	1976	955748
rapid separation of cobyrinic acid and its biosynthetic precursors by ion-exchange paper chromatography.		1976	962120
inhibition of acetate and propionate formation upon aeration of resting cells of the anaerobic propionibacterium shermanii: evidence of the pasteur reaction.	when resting cell suspensions of the anaerobic p. shermanii were brought to an oxygen concentration of 0.64 mumoles/ml, acid formation was completely inhibited. the cells started to respire on the propionic acid previously accumulated furing anaerobiosis. glucose consumption was concomitantly decreased to about 60 percent of the rate during anaerobiosis. as the viability of the cells was not affected by the transition to aerobic conditions, the changes observed upon aeration were ascribed to the ...	1976	969569
identification of propionibacterium acnes and related organisms by precipitin tests with trichloroacetic acid extracts.	the serological identification of propionibacterium acnes, p. granulosum, and p. avidum, using trichloroacetic acid extracts, is described. with antisera prepared against reference strains, the method has been tested on 142 strains recently isolated from human skin. all except two of the strains could be identified serologically, and there was excellent agreement between the serological results and the fermentation pattern of the strains. two serological types of p. acnes and two of p. avidum we ...	1976	972178
immunological enhancement of leukemia l1210 by corynebacterium parvum in allogeneic mice.	the effect of corynebacterium parvum on the immune response of c57bl/6 mice (h-2b) to the allogeneic leukemia l1210 (h-2d) was investigated. mice were either left untreated or given c. parvum i.v. or i.p. in various dosages. seven days later they were challenged with 2.5 to 10 x 10(6) live l1210 cells i.p. control animals almost always rejected the challenge. in contrast, most mice pretreated with either 1.0, 0.5, or 0.25 mg of c. parvum i.v. and 1.0 or 0.5 mg i.p. exhibited enhanced growth of l ...	1976	975053
systemic treatment of breast cancer: past, present and future.		1976	977407
mechanisms of antitumor action of corynebacterium parvum: the generation of cell-mediated tumor specific immunity.		1976	978659
comparison of the potentiation of specific tumor immunity in mice by corynebacterium parvum or bcg.	two independent studies have compared killed c. parvum (cp) vaccine with viable bcg (pasteur) and bcg (glaxo) vaccines, respectively, for potentiation of antitumor immunity when injected with irradiated tumor cells into b6d2f1 and cba-t6t6 mice. both studies concurred that, for a given number of irradiated tumor cells, larger amounts (dry weight equivalent) of bcg than cp were required to produce similar degrees of tumor immunity. evidence also showed that stronger and more protracted immunity w ...	1976	978769
biotyping and serotyping of propionibacterium acnes strains isolated from different diagnostic materials.	fifty strains of propionibacterium acnes, five strains with the previous designation corynebacterium parvum and three strains of propionibacterium avidum were agglutinated by bacto-corynebacterium acnes 554 antiserum (difco); it was not possible to isolate any strain reacting only with 605 antiserum. only one strain of c. parvum reacted with both sera. 48 (88 per cent) out of 54 strains (p. acnes and c. parvum) belonged to voss' group i and 25 (47 per cent) belonged to pulverer's biotype a. str ...	1976	983535
antimicrobiol susceptibility of propinibacterium acnes and related microbial species.	the minimal inhibitory concentrations of 32 antimicrobial agents were established for 73 strains of propionibacterium acnes and four related species (p. granulosum, p. avidum, corynebacterium minutissimum, and c. parvum). most strains showed good susceptibility to those agents usually considered active against gram-positive organisms. with the exception of c. minutissimum, the strains tested revealed more or less identical susceptibility ranges. the lowest minimal inhibitory concentrations were ...	1976	984781
the characteristics of binding of corynebacterium parvum to glass-adherent mouse peritoneal exudate cells.	corynebacterium parvum, strain 10390, whole organisms were shown to bind to the surface of glass-adherent mouse peritoneal exudate cells in vitro. an hcl extract and a lipid extract of the organism were both capable of inhibiting this binding. the attachment of organisms was not affected by trypsin treatment of the cells, indicating that the plasma membrane receptor is not cell-bound antibody in nature. the binding was inhibited by various sugars, most of which are major components of the cell w ...	1976	991461
effects of corynebacterium parvum treatment and toxoplasma gondii infection on macrophage-mediated cytostasis of tumour target cells.	injection of mice with corynebacterium parvum or living or killed toxoplasma gondii was studied to determine the efficacy of these treatments in activating peritoneal macrophages to inhibit the uptake of [3h]tdr (cytostasis) by tumour target cells in vitro. in the presence of activated macrophages from mice treated i.p. with a wide dose range of either c. parvum or living toxoplasma, cytostasis was usually greater than 99%. this population of activated macrophages was transient in c. parvum-trea ...	1976	992714
experimental cancer immunotherapy: comparison of tumor rejection if f344 rats given live mycobacterium bovis (strain bcg) and killed corynebacterium parvum.	f344 rats received grafts of syngeneic 13762 mammary adenocarcinoma cells previously admixed with either living bcg of killed corynebacterium parvum administered sc or intradermally (id). animals given id transplants of tumor cells admixed with either bcg or killed c. parvum exhibited tumor growth for an average of 10 days, then regression in size and rejection of the tumor nodules. lesions were found in rats given sc transplants of tumor cells admixed with the killed microorganism for an averag ...	1976	994209
antitumor activity of killed corynebacterium parvum suspensions in a murine mammary adenocarcinoma cad2) system.	we studied the antitumor activity of killed corynebacterium parvum on the cad2 mammary adenocarcinoma in dba/2 mice. intratumor treatment had little or no effect on subcutaneous tumor growth. admixture of tumor cells with c. parvum before inoculation completely suppressed tumor growth. no tumor transplantation immunity was detected in mice inoculated with admixtures of c. parvum and tumor cells, but tumors were enhanced under certain circumstances. growth of a tumor inoculated sc or iv was consi ...	1976	994219
complement activation in vivo in cancer patients receiving c. parvum immunotherapy.	serum complement levels were assayed in 26 patients with disseminated cancer, who received immunotherapy with infusion of c. parvum. complement activation, indicated by the consumption of c3 or c4 or both, was found in 46% of the patients. serum samples showed direct correlation between decreased c3 and conversion of c3 proactivator, whereas such conversion did not occur when c4 alone was decreased. it is concluded that the bypass (properdin) pathway was activated in patients in whom c3 consumpt ...	1976	999783
decreased liver and lung drug-metabolizing activity in mice treated with corynebacterium parvum.	injections of killed suspensions of corynebacterium parvum (i.p.) in young male mice were followed by time- and dose-dependent decreases in the drug-metabolizing activity of liver microsomes and lung homogenates. in vitro assays with model substrates [aminopyrine, aniline, p-nitroanisole, and benzo(a)pyrene] were used to quantitate drug-metabolizing activity. it is likely that such decreases in mixed function oxidases activity will act to significantly alter the pharmacokinetics of concurrently ...	1976	1000491
in vitro behavior of corynebacterium parvum-activated cytotoxic macrophages.		1976	1003401
further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide. iii. effect of c. parvum on cyclophosphamide metabolism.	results of investigations employing [14c]cyclophosphamide (cy) demonstrated that even though total plasma radioactivity was similar in female c3heb/fej mice treated either with cy alone or corynebacterium parvum and cy, the proportion of total activity due to nonmetabolized cy was greater when c. parvum was used. these findings indicated that the antitumor effect of c. parvum may not have been entirely due to its immunopotentiating properties but may also have been related to its effect on the m ...	1976	1003505
further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide.	the cytotoxic macrophage was further characterized as an important effector cell in the inhibition of tumor growth. when we administered rifampin (a semisynthetic antibiotic that interferes with macrophage function but not viability) with corynebacterium parvum and/or cyclophosphamide to tumor-bearing c3heb/fej female mice, the tumor growth-inhibitory effects of the c. parvum were reduced. moreover, when bone marrow cells from those animals were cultured, we found a marked decrease in the cytoto ...	1976	1003514
effects of corynebacterium parvum alone and in combination with adriamycin in experimental tumor systems.	corynebacterium parvum (c. parvum) was used in antitumor tests against four murine tumor models in b6d2f1 mice. the c. parvum was effective at all doses and schedules tested against p388 leukemia, b16 melanoma, and lewis lung carcinoma but was ineffective against l1210 leukemia. combination immunochemotherapy of p388 leukemia and lewis lung carcinoma with c. parvum and adriamycin was better than either regimen alone in increasing the lifespan of mice with tumors. the results show that the effect ...	1976	1009517
the role of nonspecific immunotherapy in the treatment of breast cancer.		1976	1013508
the immunostimulant corynibacterium parvum and hematopoietic toxicity of chemotherapy.		1976	1019832
mouse strain differences in macrophage activation and anti-tumor activity induced by propionibacterium acnes (anaerobic coryneforms).	effect of propionibacterium acnes on macrophage activation and antitumor activity was examined in ddn and sl mice. (1) carbon clearance was enhanced to the same extent by p. acnes treatment in both strains. (2) number of peritoneal macrophages increased to the same extent by p. acnes treatment in both strains. (3) adhesiveness of peritoneal macrophages, as demonstrated by inhibition or migration or an increase in spreading cells, was enhanced more efficiently in ddn than in sl mice p. acnes tre ...	1976	1021506
[effect of porphyrins and their derivatives on biosynthesis of vitamin b 12 and the development of propionibacterium shermanii].	the effect of uroporphyrin, coproporphyrin and their cobalt-containing derivatives on the biosynthesis of vitamin b12 and development of propionibacterium shermanii was studied. the compounds under study stimulated the vitamin synthesis by growing cultures and resting suspensions of these bacteria. cobalt porphyrins as the sole source of cobalt were used in the vitamin b12 biosynthesis. an addition of cobalt porphyrins to the growing culture of propionic bacteria increased in accumulation of the ...	1976	1026936
[changes of antimicrobial susceptibility of anaerobic bacteria from clinical specimens (author's transl)].	the susceptibility to several commonly used antimicrobial agents was examined on approximately 900 isolates of various anaerobes from clinical specimens between october 1965 to may 1975, and the changes of susceptibility were investigated. antimicrobial agents tested were penicillin g (pc-g), ampicillin (ab-pc), cephaloridine (cer), erythromycin (em), josamycin (jm), lincomycin (lcm), clindamycin (cldm), chloramphenicol (cp) and tetracycline (tc). and thiamphenicol (tp) and doxycycline (dotc) we ...	1975	1042183
a simple method for estimating viable filamentous bacteria in plaque.		1975	1053770
concerning the biosynthesis of vitamin b12.		1975	1056170
[management of acute leukemias and linphosarcomas with active immunotherapy (author's transl)].		1975	1056311
enhancement by drugs of metastatic lung nodule formation after intravenous tumour cell injection.	in studies on a model of induced pulmonary metastasis in mice a tumour host system was analysed which was not affected by immunogenicity of the tumour for the host; neither intensive immunosuppression nor immunization caused a significant change in the quantity of pulmonary metastatic nodules. in contrast the application of cytostatic drugs and of corynebacterium parvum could modify the pulmonary resistance to the formation of tumour nodules by a factor greater than 100 in either direction. this ...	1975	1056314
antibiotic treatment of oral anaerobic infections.		1975	1056466
new experimental and clinical data on leukaemia immunotherapy.	the present results of our treatment of acute lymphoid leukaemia patients are summarized: 7 out of 20 randomized patients given active immunotherapy after chemoradiotherapy are still in complete remission after periods varying from seven to ten years (compared to none in the control group). the actuarial results on 100 patients show remission and survival curves presenting a plateau between three and five years for a certain percentage, suggesting a possible cure. several parameters studied in 2 ...	1975	1060146
studies on the enhancement by cyclophosphamide (nsc-26271) of artificial lung metastasis after labeled cell inoculation.	in a mouse model the retention of125i-5-iodo-2' -deoxyuridine-labeled tumor cells in the lung after iv injection was compared with the formation of tumor colonies in the lung 15 days after injection. the modification in lung retention after treatment with cyclophosphamide (cp), isophosphamide, corynebacterium parvum, and heparin paralleled the changes in lung-colony numbers by these treatments. since the modifications could be identified as early as 1 hour after iv administration of tumor cells, ...	1976	1064469
macrophage mediated tumor cell cytotoxicity.	the following evidence from our research has implicated the macrophage as an important effector cell in pyran and/or c. parvum induced host resistance to solid tumors: (1) increased infiltration of tumors with histiocytes following systemic treatment with pyran;17 (2) activated peritoneal macrophages with tumoricidal activity have been recovered from the peritoneal cavity of normal or tumor bearing mice treated with pyran or c. parvum;17 (3) activated peritoneal macrophages mixed with tumor cell ...	1976	1071961
[taxonomic position of oral anaerobic diphtheroid group (author's transl)].		1975	1074278
taxonomical position of anaerobic diphtheroids from human oral cavity.		1976	1076074
mechanisms of macrophage activation by corynebacterium parvum. ii. in vivo experiments.		1975	1079165
daily intravenous infusions of corynebacterium parvum in twenty patients with disseminated cancer: a preliminary report of clinical and biologic findings.	twenty terminally ill patients with various disseminated tumors were treated with daily iv infusions of corynebacterium parvum given alone at doses of 4 mg/day, 5 days/week, for 4-16 weeks. in 8 patients (40%), the lesions partially regressed to less than 50% of their original size. another patient who did not improve with c. parvum therapy had a complete remission after the first course of chemotherapy. skin tests, total leukocyte counts, and t- and b-cell counts revealed variable and unpredict ...	1975	1080521
effect of local injection of corynebacterium parvum on the growth of a murine fibrosarcoma.	local injection (i.e. injection at the site of tumour inoculation) of strains of c. parvum which have a significant anti-tumour effect when given systemically (i.e. intravenously or, in the case of subcutaneous tumour transplant, intraperitoneally) strongly inhibits the growth of isogeneic transplants of a fibrosarcoma in intact cba mice but has little or no effect on subcutaneous transplants of the same tumour in t-cell deprived mice. the anti-tumour effect of local injection of c. parvum, unli ...	1975	1080671
active specific immunotherapy of mouse methylcholanthrene induced tumours with corynebacterium parvum and irradiated tumour cells.	the relative efficiency of active nonspecific or specific immunotherapy of developing methylcholanthrene induced fibrosarcomata with c. parvum was compared. for nonspecific immunotherapy, mice were challenged with tumour cells s.c. or i.v., and 2 days later injected i.v. with dilutions of c. parvum. the only significant effect was a retardation of s.c. tumour growth by the highest concentration of c. parvum (350 mug). however, active specific immunotherapy, using mixtures of c. parvum and irradi ...	1975	1082344
further observations on the diagnosis cause, and treatment of endophthalmitis.	in our study of 54 suspected cases of endophthalmitis, vitreous aspiration was more sensitive in making a culture-proven diagnosis than anterior chamber paracentesis; staphylococcus epidermidis was a more common cause of endophthalmitis than previously appreciated; and intraocular antibiotics in the recommended dosage are reasonably safe clinically and add a new dimension to the treatment of endophthalmitis.	1976	1082716
immunotherapy: concepts and nursing implications.	concepts of immunlogy and the human immune system are essential for an understanding of the rationale of immunotherapy. immunotherapy is presently being investigated as adjunct therapy for patients with cancer. it is therefore essential for the professional nurse to become cognizant of the various modalities and possible complications of immunotherapy in order that the nursing implications may be incorporated into clinical practice.	1976	1083016
the accumulated effects of repeated systemic or local injections of low doses of corynebacterium parvum in mice.	the effects of 14 weekly injections, s.c. or i.v., of "human equivalent" doses (5.25 mg/sq m) of corynebacterium parvum (cp) in mice have been compared. both s.c. and i.v. cp caused significant splenomegaly and antibody to cp, but stimulation was considerably greater after i.v. cp. delayed hypersensitivity levels to cp were similar after s.c. and i.v. injection. t-cell competence, as judged by phytohemagglutinin reactivity and delayed hypersensitivity to sheep cells, was unimpaired after s.c. cp ...	1976	1083289
effect of corynebacterium parvum on cellular and humoral antitumoral immune effector mechanisms.		1976	1083341
the effect of corynebacterium parvum on the humoral and cellular immune systems in patients with breast cancer.	corynebacterium parvum, a gram-positive anaerobic bacillus thought to be a strong immunological stimulant, has been shown to decrease tumour growth and prolong survival in patients with metastatic disease. study of the effect of a single injection of a strain of c. parvum (cn. 6134) in six patients with stage iv metastatic breast cancer is reported. results of laboratory tests to judge the physical and immunological effects of the drug infusion 24 hr post-treatment and weekly thereafter for 3 we ...	1976	1084821
abrogation of the anti-metastatic activity of c. parvum by antilymphocyte serum.		1976	1086096
mechanisms of antitumor action of corynebacterium parvum: replicating short-lived t cells as the mediators of potentiated tumor-specific immunity.		1976	1086362
lactate metabolism in propionibacterium pentosaceum growing with nitrate or oxygen as hydrogen acceptor.	when anaerobic cultures of propionibacterium pentosaceum were shifted to low dissolved-oxygen concentration (d.o.c.), acetate production from lactate diminished and propionate production stopped, whereas pyruvate accumulated and oxygen was consumed. assuming that energy is generated in the electron transfer to oxygen, yatp values (g dry wt bacteria/mole atp) of between 7.2 and 11.9 were calculated from molar growth yields and product formation. when oxidative phosphorylation in the electron tran ...	1976	1086638
osteosarcoma: a reconnaissance.		1975	1091399
mechanisms of macrophage activation by corynebacterium parvum. i. in vitro experiments.		1975	1092470
effect of corynebacterium parvum on the class and subclass of antibody produced in the response of different strains of mice to sheep erythrocytes.	several strains of mice were injected with sheep erythrocytes (srbc) using c. parvum as adjuvant. the adjuvant effects on the amounts of class and subclass of antibody produced were ranked in the order igg2b greater than igg2a and igm greater than igg1. in addition, these effects were shown to vary depending on the time of administration of c. parvum relative to antigen. c parvum was shown to have no adjuvant effect on the response of congenitally athymic mice when given at the same time as the ...	1975	1092609
rejection of mammary adenocarcinoma cell tumors in dba/2 mice immunocompromised by thymectomy and treatment with antithymocyte serum.	syngeneic mammary adenocarcinoma cells were mixed with killed corynebacterium parvum organisms and were then transplanted subcutaneously into groups of normal and immunocompromised mice. the tumors formed at the site of injection exhibited normal growth for approximately 12 days followed by rapid and lasting rejection in both normal and immunocompromised animals. the control mice were protected against reinjections of 10-4 to 10-8 cells of the same line of tumor cells. the immunocompromised mic ...	1975	1092759
tumor inhibitory properties of anaerobic corynebacteria.		1975	1093294
suppression of the immune response by microorganisms.		1975	1095003
[a study on the nature of corynebacterium acnes (author's transl)].		1975	1099281
the role of macrophages in the amplified in vitro response to sheep red blood cells by spleen cells from corynebacterium parvum treated mice.		1975	1102113
nonspecific enhancers of resistance in man.	nonspecific enhancers of resistance may include (1) viral interference, (2) interferon, (3) interferon inducers, (4) bacterial interference, (5) bacterial products such as coley's "toxins," endotoxins, or staphylococcal, bcg, and corynebacterium parvum vaccines, (6) transfer factor, and (7) well-defined chemicals such as dinitrochlorbenzene, levamisole, and vitamin c. these are discussed only as they have been applied to man to learn whether or not they have enhanced his ability to resist infect ...	1975	1102645
evaluation of a test kit for identification of anaerobic bacteria.	a test kit for identification of anaerobic bacteria--api--has been compared for accuracy in individual tests and for identification on the genus or species level with pre-reduced anaerobically sterilized media methods on 241 anaerobic strains. the microsystem was found to be reliable and permits identification of the clinically most significance anaerobic bacteria if it is supplemented with other tests such as gaschromatographic analysis, morphology, lecithinase, lipase and antibiotic sensitivit ...	1975	1105128
structural properties of pyruvate carboxylases from chicken liver and other sources.	varieties of pyruvate carboxylase [pyruvate: co2 ligase (adp-forming), ec 6.4.1.1] obtained from the livers of several species of vertebrates, including humans, all show the same basic structure. they are composed of large polypeptide chains of molecular weights ranging from 1.2 to 1.3 x 10(5) for the different varieties of the enzyme. the native form of the enzyme appears to be a tetramer with a molecular weight of about 5 x 10(5). in the case of pyruvate carboxylase from chicken liver each pol ...	1975	1105579
effects of bcg, corynebacterium parvum, and methanol-extration residue in the reduction of mortality from staphylococcus aureus and candida albicans infections in immunosuppressed mice.	an immunosuppressed mouse model was devised to test the effects of immunopotentiators on the prevention of bacterial and fungal infections. the effects of bcg and corynebacterium were tested against staphylococcus aureus and candida albicans infection. the effect of methanol-extraction residue (mer-bcg) was tested against s. aureus septicemia. cdf mice were given various doses of bcg, 1.0 mg of c. parvum, or 0.5 mg of mer intraperitoneally at varying intervals before injection of an intravenous ...	1975	1107224
inhibitory effect of prolonged corynebacterium parvum and cyclophosphamide administration on the growth of established tumors.	the present study was carried out to investigate the effects of prolonged administration of c. parvum alone and in combination with cyclophosphamide for the treatment of established, measurable c3h tumors. the continued weekly administration of c. parvum by itself provided a limited but significant inhibitory effect on tumor growth and significantly prolonged survival. intraperitoneal and intravenous administration was found to be more effective than the subcutaneous route. when c. parvum was ad ...	1975	1109767
specific and nonspecific stimulation of resistance to the growth and metastasis of the line 1 lung carcinoma.	the effects of specific dead tumor cell immunization and nonspecific immunostimulation with corynebacterium parvum on the s.c. growth of the line 1 carcinoma in syngeneic balb/c mice have been studied. injection of heavily irradiated line ) carcinoma cells did not inhibit the transplantability or growth of the line 1 carcinoma, and in certain cases these treatments actually prolonged the period of rapid growth. this latter observation was traced to a mild inhibition of metastatic spread, which i ...	1975	1109793
purification of the subunits of transcarboxylase by affinity chromatography on avidin-sepharose.	transcarboxylase consists of a central 12 sh subunits each of which is linked to the central subunit by two similar to 1.3 se biotin carboxyl carrier proteins. the subunits from dissociated transcarboxylase have been difficult to isolate because conditions which stabilize them also promote their reassociation to the intact enzyme. in this paper, we describe the use of avidin-sepharose to adsorb the enzyme from crude extracts or partially purified transcarboxylase of propionibacteria. after remov ...	1975	1112797
proceedings: combined surgery and corynebacterium parvum for treatment of metastasizing tumour in mice.		1975	1115892
proceedings: the action of subcutaneous and intravenous corynebacterium parvum on the "lymphocyte trap".		1975	1115893
a comparative study of anaerobic coryneforms. attempts to correlate their anti-tumour activity with their serological properties and ability to stimulate the lymphoreticular system.	various strains of anaerobic coryneforms and the closely related propionibacteria have been compared in vivo with respect to their anti-tumour activity. their effectiveness has been correlated with their serological relationship and to some extent with their ability to stimulate the lymphoreticular system. organisms belonging to corynebacterium acnes groups i and ii and c. avidum group iv were active anti-tumour agents, although of varying effectiveness. these strains are serologically closely r ...	1975	1116870
enzymatic determination of microquantities of acetate.		1975	1121284
effect of corynebacterium parvum on bone marrow cell cultures (38557).	the effect of c. parvum on in vitro colony-forming cell was evaluated. a single ip injection of the vaccine had no effect during the first 4 hr either on colony stimulating factor or on colony-forming cells. the enhancement of the colony-forming capacity of the bone marrow by c.parvum occurred after two injections of the vaccine within 48 hr. the stimulation of colony formation by c. parvum was time related function and occured 5 days after the initiation of the cultures. the results of this ...	1975	1121493
isolation of peptides from the carboxyl carrier subunit of transcarboxylase. role of the non-biotinyl peptide in assembly.	transcarboxylase is made up of a central hexameric subunit (s20,w similar 12 s), three peripheral dimeric metallo subunits (s20,w similar to 5 s), and six biotinyl carboxyl carrier subunits (s20,w similar to 1.3 s). the results presented here show that the carboxyl carrier subunit is required for assembly of the 12s and 5s subunits into the oligomer. however, only a portion of the subunit is required for assembly. on treatment of transcarboxylase briefly with trypsin at ph 6.3 extremely suscepti ...	1975	1125190
evidence that the two partial reactions of transcarboxylation are catalyzed by two dissimilar subunits of transcarboxylase.	the results presented here show that isolated subunits of transcarboxylase specifically catalyze the two partial reactions of transcarboxylation as shown in eq 1-3. the 12s central subunit is active in the transcarboxylation with methylmalonyl-coa but inactive with oxalacetate and the peripheral metallo 5s subunit is active in the transcarboxylation with oxalacetate but inactive with methylmalonyl-coa. these subunits, likewise, are specific for the reverse partial reactions; the central subunit ...	1975	1125191
letter: concerning the intermediacy of uro'gen iii and of a heptacarboxylic uro'gen in corrinoid biosynthesis.		1975	1133422
cancer immunology.		1975	1135940
diphtheroid infection of the cervical spine.	a unique case in which cervical osteomyelitis was caused by a diphtheroid is reported and the previously recorded clinical infections due to similar organisms are briefly reviewed.	1975	1136738
radiation response of c3h fibrosarcoma enhanced in mice stimulated by corynebacterium parvum.		1975	1138200
the in vitro killing of syngeneic cells by peritoneal cells from adjuvant-stimulated mice.		1975	1139640
esterification of (4--14c)cholesterol by cutaneous bacteria (staphylococcus epidermidis, propionibacterium acnes, and propionibacterium granulosum).	cholesterol and cholesteryl esters constitute 3 to 5% of the lipids derived from human sebaceous glands. the present study demonstrates that cutaneous bacteria, staphylococcus epidermidis, and to a lesser extent propionibacterium acnes, and propionibacterium granulosum, esterify [4--14c]cholesterol to steryl esters in vitro. the degree of esterification is a characteristic of the strain of organism, ranging from 0 to 32.7% in the organisms tested. cholesterol esterification in these experiments ...	1975	1141713
further analysis of the anti-tumour effect in vitro of peritoneal exudate cells from mice treated with corynebacterium parvum.	administration of c. parvum to both intact and thymectomized mice resulted in the appearance in the peritoneal exudate of cells which inhibited tumour growth in vitro. this effect was mediated by intact, viable adherent cells, which it seems reasonable to categorize as macrophages, and was contingent on contact between the effector and target cells. no co-operation was observed between lymph node cells from c. parvum treated mice and peritoneal exudate cells from normal mice.	1975	1156505
lack of immunological and anti-tumour effects of orally administered corynebacterium papvum in mice.		1975	1156520
stereochemistry of propionyl-coenzyme a and pyruvate carboxylations catalyzed by transcarboxylase.	the stereochemistry of the two half-reactions catalyzed by the biotin-containing enzyme, transcarboxy-lase from propionobacteria shermanii, has been determined. the pro-r hydrogen at c-2 of propionyl-coenzyme a is replaced by co2 in formation of the s isomer of methylmalonyl-coa, defining the process as retention of configuration. this c-2 hydrogen is abstracted at a rate identical with product formation. for the other half-reaction, pyruvate to oxalacetate, the chiral methyl group methodology o ...	1975	1156576
modifications in the handling in vitro of 125i-labelled keyhole limpet haemocyanin by peritoneal macrophages from mice pretreated with the adjuvant corynebacterium parvum.	peritoneal macrophages were isolated from c. parvum-pretreated (cp) and normal cbat6t6 mice and their in vitro handling of 125i-labelled keyhole limpet haemocyanin (125i-labelled klh) studied in relation to the humoral anti-klh responses induced in corresponding animals. cp pretreatment exerted an adjuvant effect on the production of anti-klh antibodies, both igm and igg, which was also demonstrable with a normally subimmunogenic dose of antigen. there was a clear difference between the handling ...	1975	1158386
[oxidative phosphorylation in propionibacterium].	oxidative phosphorylation during electron transport in the respiratory chain was found in two propionic bacteria, p. shermanii and p. petersonii. its effectiveness, with oxygen as the terminal acceptor of electrons, was higher in p. petersonii, a more aerobic culture, than in p. shermanii. oxidative phosphorylation with the participation of the electron transport chain was not found in p. petersonii in the absence of oxygen. oxidative phosphorylation can take place together with the reactions of ...	1975	1160625
proceedings: treatment of a metastasizing murine tumour with corynebacterium parvum.		1975	1164486
evaluation of human oral organisms and pathogenic streptococcus for production of iga protease.	iga protease is a proteolytic enzyme found in whole human saliva and in dental plaque that cleaves both secretory and myeloma iga of human origin to yield intact fabalpha and fcalpha fragments. to determine which bacteria are capable of producing this enzyme, we have examined a variety of strains normally found in the human oral cavity and a number of streptococci of known lancefield group serotype. streptococci of groups a, b, c, d, f, g, h, m, and n, streptococcus mutans, streptococcus sanguis ...	1975	1168676
serological studies of actinomyces israelii by crossed immunoelectrophoresis: taxonomic and diagnostic applications.	crossed immunoelectrophoresis (cie) with intermediate gel was applied to the serological analysis of actinomyces israelii to develop a test with high efficiency in the laboratory diagnosis of human actinomycosis and classification of a. israelii. recently developed standard antigen-antibody systems for a. israelii by cie were used as reference. the reference systems were based on standard preparations of cytoplasmic and whole cell-associated antigens of a. israelii and a standard immunoglobulin ...	1975	1171825
role of cellular proliferation in the stimulation of mps phagocytic activity.	administration of agents such as stilboestrol, endotoxin, c. parvum and zymosan, which enhance the phagocytic activity of the mononuclear phagocyte system, also stimulated cellular proliferation in the liver and spleen as assessed by [13h] thymidine incorporation into these organs, and by autoradiography and the number of mitoses in the liver. sublethal whole body irradiation abolished the proliferative response to stimulation and also reduced or prevented the increase in phagocytic activity. th ...	1975	1174456
bacteriology of normal and infected conjunctiva.	the conjunctivas of 273 inflamed eyes were cultured by both aerobic and anaerobic techniques. isolations were obtained from 267 (97.8%) of the eyes. aerobic organisms were isolated from 237 (86.8%) of the conjunctivas. staphylococcus aureus from 63 (23.1%) of the diseased eyes was the aerobic pathogen most often isolated. anaerobic bacteria were isolated from 172 (63.0%) of the conjunctivas. propionibacterium acnes was isolated from 126 (46.2%) of the conjunctivas, and peptostreptococcus species ...	1975	1176598
splenic suppressor macrophages induced in mice by injection of corynebacterium parvum.	spleen cells from c57bl/6n mice injected with killed corynebacterium parvum (cp) had a marked growth inhibitory effect on the in vitro proliferation of rbl-5 murine lymphoma cells. it was most marked 12 to 14 days after injection and was usually no longer detectable later than 21 days. it could be demonstrated at effector cell to target ratios between 20:1 and 5:1 at which normal spleen cells had a growth-promoting effect. addition of cp to an in vitro mixture of spleen cells and tumor cells aug ...	1975	1176773
age-related changes in the resident bacterial flora of the human face.	quantitative levels of resident aerobic and anaerobic bacteria of the face, show a characteristic age-related pattern. the density of anaerobic diptheroids and surface aerobic micrococci is higher in infancy than in early childhood. at puberty the quantity of organisms increases, with significantly higher levels achieved in late adolescence. maximum counts are attained in early adulthood and remain constant until old age when a trend toward lower numbers occurs. these changes seem to correlate w ...	1975	1176788
infection after total knee arthroplasty.		1975	1178156
combination of c. parvum and specific immunization against artificial pulmonary metastases in mice.	we have studied whether specific immunization administered intraperitoneally can augment the activity of c. parvum (0.25 mg intraperitoneally or intravenously) against intravenously injected cells of a syngeneic fibrosarcoma in c3hf/bu mice as expressed by the reduction of pulmonary metastases (nodules, colonies) and/or by the prolongation of the survival of recipients. combination of specific immunization and c. parvum, applied either before or after iv inoculation of viable tumor cells, was mo ...	1975	1184241
[letter: immunotherapy in malignant melanoma].		1975	1188912
studies on bacteriophages of propionibacterium acnes.	with the help of adaptation experiments, 61 phage preparations out of 36 propionibacterium acnes bacteriophages (32 isolated by us and 4 sent from abroad) were established. on the basis of their stability, spectrum of activity, and virulence, 13 phages were selected for phagetyping. 58 well-classified p. acnes strains were grouped into 7 phage-types. 7 strains of p. granulosum, two strains of p. avidum, and one yet ungroupable microserophilic propionibacterium strain were resistant to all 61 pha ...	1975	1196261
parameters of anaerobiosis of propionibacterium shermanii and clostridium sticklandii.		1975	1199136
numerical taxonomy and laboratory identification of actinomyces and arachnia and some related bacteria.	a numerical taxonomic study was made on 49 facultative anaerobic gram-positive filamentous and/or diphtheroidal organisms isolated from dental plaques, carious dentin and faeces, together with 63 reference strains belonging to the genera actinomyces, arachnia, bifidobacterium, actinobacterium, propionibacterium, eubacterium and lactobacillus. they were examined for 90 unit characters covering a wide range of tests and properties. the data were subjected to computer analysis in which the simple m ...	1975	1202157