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comparison of three dna extraction methods for detection of erysipelothrix rhusiopathiae in chicken blood by polymerase chain reaction.a previously reported erysipelothrix-specific polymerase chain reaction (pcr) was used to detect erysipelothrix bacteremia in chickens. the sensitivity of pcr using 3 dna extraction methods (boiling method, commercial gene matrix, and dna extractor kit) was compared by using a serial 10-fold dilution of a chicken isolate of erysipelothrix rhusiopathiae strain in chicken blood. of the techniques used, the dna extractor kit, followed by pcr, provided the most sensitive method for the detection of ...200919407089
oral vaccination against mycoplasmal pneumonia of swine using a live erysipelothrix rhusiopathiae vaccine strain as a vector.erysipelothrix rhusiopathiae koganei 65-0.15 strain, the live swine erysipelas vaccine for subcutaneous injection, has been shown to colonize the tonsils of pigs after oral inoculation. we thus evaluated the possible use of the strain as a vector for oral vaccination against mycoplasmal pneumonia of swine. recombinant e. rhusiopathiae strains expressing the c-terminal domain of the p97 adhesin of mycoplasma hyopneumoniae were constructed and examined for vaccine efficacy in mice and pigs. mice s ...200919433128
erysipelothrix rhusiopathiae intra-abdominal abscess.erysipelothrix rhusiopathiae is a gram-positive bacillus that is infrequently responsible for infections in humans. most human cases present as localized or generalized cutaneous infections. an invasive septic form, usually associated with endocarditis, has rarely been described. we report here an invasive infection caused by e. rhusiopathiae without endocardium involvement. to our knowledge, this is the first report of an intra-abdominal abscess due to this pathogen.201019481488
endocarditis in a british heifer due to erysipelothrix rhusiopathiae infection. 200919578194
etiological and biological characteristics of erysipelothrix rhusiopathiae isolated between 1994 and 2001 from pigs with swine erysipelas in japan.we investigated 66 erysipelothrix rhusiopathiae strains isolated from pigs affected with swine erysipelas in japan from 1994 to 2001 for serotype, pathogenicity towards mice, protection in vaccinated mice and antimicrobial susceptibility. most of the isolates (84.8%) were serotype 1 or 2. for the first time, strains belonging to serotype 21 were isolated from cases of septicemia. fifty isolates (75.8%) were highly virulent, 12 isolates (18.2%) were weakly virulent and 4 isolates were avirulent s ...200919578275
erysipeloid: a review.erysipeloid is an occupational infection of the skin caused by traumatic penetration of erysipelothrix rhusiopathiae. the disease is characterized clinically by an erythematous oedema, with well-defined and raised borders, usually localized to the back of one hand and/or fingers. vesicular, bullous and erosive lesions may also be present. the lesion may be asymptomatic or accompanied by mild pruritus, pain and fever. in addition to cutaneous infection, e. rhusiopathiae can cause endocarditis, wh ...200919663854
erysipelothrix rhusiopathiae.erysipelothrix rhusiopathiae is a facultative, non-spore-forming, non-acid-fast, small, gram-positive bacillus. the organism was first established as a human pathogen late in the nineteenth century. three forms of human disease have been recognised since then. these include a localised cutaneous lesion form, erysipeloid, a generalised cutaneous form and a septicaemic form often associated with endocarditis. the organism is ubiquitous and able to persist for a long period of time in the environme ...201019733019
development of quantitative real-time polymerase chain reaction for detection of and discrimination between erysipelothrix rhusiopathiae and other erysipelothrix species.quantitative real-time polymerase chain reaction (qpcr) assays were developed and validated in combination with enrichment culture for the detection and discrimination of erysipelothrix rhusiopathiae and other erysipelothrix species from tissue samples. the targets for sybr green qpcr assays were the 16s ribosomal rna gene for erysipelothrix species and a gene involved in capsular formation for e. rhusiopathiae. the specificity of the assays was assessed with erysipelothrix species and other rel ...200919737769
experimental study on possible transmission of the bacterium erysipelothrix rhusiopathiae to chickens by the poultry red mite, dermanyssus gallinae.the vector potential of the poultry red mite, dermanyssus gallinae de geer (acari: dermanyssidae), in relation to chicken erysipelas was investigated under experimental conditions. chickens were inoculated intramuscularly with the bacterium erysipelothrix rhusiopathiae, and mites were allowed to feed on the inoculated chickens for 5 days. after 20 days of starvation, the mites were allowed to feed on healthy chickens to enable transmission of bacteria. blood samples were collected from the birds ...201019777357
rapid detection and differentiation of erysipelothrix spp. by a novel multiplex real-time pcr assay.the aim of this study was to develop a multiplex real-time pcr assay for the identification and discrimination of erysipelothrix rhusiopathiae, tonsillarum and erysipelothrix sp. strain 2 for direct detection of erysipelothrix spp. from animal specimens.201019840181
changes of immunophysiological characteristics in neonatal calves experimentally challenged with mixture of live bacteria and virus.the aim of the present study was to define efficient immunophysiological parameters in neonatal holstein calves with an experimentally induced microbial infection. calves (n = 15) were challenged with classical swine fever virus (lom strain) and erysipelothrix insidiosa live vaccine by intravenous injection at 3 wk of age except for control calves (n = 4). the level of total serum iga was significantly increased at 14 and 19 d post-experimental challenge (dpec) compared with that in calves at -2 ...200919841216
[enhancement of antibodies to protective domain of surface protective antigen a of erysipelothrix rhusiopathiae by dna immunization with plasmids expressing spaa-chimeras].dna vaccines expressing protective domain of surface protective antigen a(spaa)of erysipelothrix rhusiopathiae have been relatively ineffective at generating high-titer, long-lasting, neutralizing antibodies in murine models.200919900362
septic arthritis caused by erysipelothrix rhusiopathiae in a prosthetic knee joint.we describe the third case of prosthetic infection due to erysipelothrix rhusiopathiae. the patient, a 68-year-old woman, had had total knee arthroplasty 12 months before diagnosis. she had been in contact with swine at home. we review the seven previous reports of septic arthritis due to e. rhusiopathiae.201019923477
conjugative transposition of tn916 and detection of tn916-like transposon in erysipelothrix rhusiopathiae.in order to investigate the origin of tetracycline resistance in erysipelothrix rhusiopathiae, conjugative transpositions of tn916 were tested. the frequency of transfer between strains of e. rhusiopathiae was about 10-fold higher than that between enterococcus faecalis and e. rhusiopathiae. in addition, detection of a tn916-like transposon was performed by pcr assay and dna sequencing in e. rhusiopathiae field isolates. of 49 tetracycline-resistant isolates, 38 (77.6 %) carried a tn916-like tra ...200919959909
septic polyarthritis caused by erysipelothrix rhusiopathiae in a dog.a 14-month-old, male german shepherd dog was admitted with a six-week history of lameness and swelling of the right hindlimb. clinical examination revealed polyarthritis, fever, petechiae and ecchymoses of the abdominal skin and prepuce. the haematology and blood chemistry were indicative of sepsis. mediolateral radiographic views of both of the stifle joints revealed signs of bilateral articular capsule swelling. the radiographical, bacteriological and necropsy findings confirmed a diagnosis of ...201019997677
antimicrobial susceptibility of erysipelothrix rhusiopathiae isolated from pigs in southern japan with a modified agar dilution method.the determination of antimicrobial minimum inhibitory concentration (mic) in erysipelothrix rhusiopathiae by using the agar dilution method has not been covered by the clinical and laboratory institute (clsi). only the broth microdilution method has been outlined. this report describes a modification of the agar dilution procedure for e. rhusiopathiae using trypto-soy agar supplemented with 0.1% tween 80 and incubation in ambient air at 37 degrees c for 24 hr. the mics of the assay were in agree ...201020068270
development and validation of an immunohistochemical method for rapid diagnosis of swine erysipelas in formalin-fixed, paraffin-embedded tissue samples.the objective of the study was to develop an immunohistochemical (ihc) assay for rapid detection of erysipelothrix rhusiopathiae. serotypes 1a, 1b, and 2 are most frequently associated with clinical disease in pigs. antiserum against serotypes 1a, 1b, and 2 was produced in rabbits, pooled, and applied to formalin-fixed, paraffin-embedded tissue sections of pigs (lungs, heart, spleen, and skin). the results obtained with the ihc assay were compared with direct culture on tissue samples from exper ...201020093690
erysipelothrix rhusiopathiae: association of spa-type with serotype and role in protective immunity.a collection of swine, fish, and cetacean erysipelothrix rhusiopathiae strains representing 16 serotypes was analyzed for possession of the three currently recognized surface protective antigen (spa)-types: spaa, spab, and spac. polymerase chain reaction (pcr) assays and western blotting with a spaa-specific monoclonal antibody demonstrated that spa-type is not confined to specific serotype groups. in particular, the spa-type of strains of aquatic origin was more variable than those of terrestri ...201020117264
cloning, expression, purification, crystallization and preliminary x-ray diffraction analysis of the collagen-binding region of rspb from erysipelothrix rhusiopathiae.rspb is a surface adhesin of erysipelothrix rhusiopathiae. a recombinant form of the collagen-binding region of this protein, rspb((31-348)), has been overexpressed in escherichia coli in native and selenomethionine-derivative forms and purified using affinity and gel-permeation chromatography. thin plate-like crystals were obtained by the hanging-drop vapour-diffusion method using the same condition for both forms. the native crystals diffracted to a resolution of 2.5 a using an in-house x-ray ...201020124711
[baker rosenbach erysipeloid appearing as a granulomatous cheilitis].baker-rosenbach's erysipeloid is a skin infection caused by erysipelothrix rhusiopathiae. it occurs essentially in humans exposed to animals colonized with this germ such as swine. the typical skin lesion, an erythematous macule generally localized to inoculation site, frequently in the extremities, quickly resolves spontaneously. the lips are an atypical site of this infection. we describe a case of chronic granulomatosis cheilitis in a farmer caused by e. rhusiopathiae.201020171435
[septic arthritis caused by erysipelotrix rhusiopathiae: case report and review of the literature].a 68-year-old man presented at our department with a suspected bacterial arthritis of the left knee. all diagnostic measures confirmed this diagnosis and the detected germ was erysipelothrix rhusiopathiae. after arthroscopic debridement of the left knee, intravenous and oral antibiotic treatment for a total of 6 weeks, the patient was treated by total knee arthroplasty 6 months after the first presentation. the postoperative follow-up was without any pathological findings. there are only 4 other ...200920183753
streptomycin in the treatment of erysipelothrix rhusiopathiae-infected turkeys. 194720244039
penicillin and streptomycin in the treatment of experimental erysipelothrix rhusiopathiae infection of mice; with observations on immunologic reaction to the infection. 194620244436
some properties of a thermolabile antigen of erysipelothrix rhusiopathiae. 194720251281
the common rat as a source of erysipelothrix rhusiopathiae. 194720252863
sulphonamide investigations; treatment of experimental pasteurella septica, ery. rhusiopathiae and s. cholerae-suis infections in mice with sulphathiazole, sulphadiazine, sulphamezathine and sulphapyridine. 194720265764
erysipelothrix rhusiopathiae infection in man; report of a case with cutaneous bullae, in which cure was achieved with penicillin. 194620276372
effects of penicillin on erysipelothrix rhusiopathiae and on mice infected with that organism. 194720279634
penicillin in the treatment of erysipelothrix rhusiopathiae-infected turkeys. 194720294062
chemotherapy of erysipelothrix rhusiopathiae infections in mice with streptomycin. 194720295952
identification of surface protective antigen (spa) types in erysipelothrix reference strains and diagnostic samples by spa multiplex real-time and conventional pcr assays.to develop spa multiplex real-time and conventional pcr assays to detect and differentiate between spaa, spab and spac genes within erysipelothrix spp.201020477888
a commendation on "septic polyarthritis caused by erysipelothrix rhusiopathiae in a dog". 201020485860
[immunity of native spaa and recombinant spaa-n against erysipelothrix rhusiopathiae infection in mice].we evaluated the effects of surface protective antigen a (spaa) and its n-teminal protective domain (rspaa-n) against erysipelothrix rhusiopathiae infection in mice.201020499642
cellular immune responses in cetaceans immunized with a porcine erysipelas vaccine.clinical erysipelas represents a significant health problem in managed cetacean species. vaccination was suspended in many oceanariums in the past due to losses associated with vaccine-induced hypersensitivities which were deemed to be a greater threat than clinical erysipelas. a perceived shift in clinical presentation of erysipelas from a chronic dermatologic form to an acute systemic form in dolphins sparked interest in re-initiating vaccination with improved subunit vaccines of erysipelothri ...201020579745
erysipelothrix rhusiopathiae peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis.erysipelothrix rhusiopathiae is known as a pathogen of occupational diseases or a zoonosis. we report a case of e. rhusiopathiae peritonitis in a 50-yr-old male undergoing continuous ambulatory peritoneal dialysis (capd). he was suffered from mild abdominal pain with a distinctive erysipeloid skin lesion. e. rhusiopathiae was considered to be introduced through a lacerated wound on his hand when he was exposed to contaminated materials. he was treated successfully with a first generation cephalo ...201020676340
characterization and identification of erysipelothrix rhusiopathiae isolated from an unnatural host, a cat, with a clinical manifestation of depression.erysipelothrix rhusiopathiae is pathogenic for humans, many domestic animals and wild birds, but infectious cases with clinical symptoms in cats have not been reported. e. rhusiopathiae was recovered from a 4-month russian blue breed cat with a very poor body condition score of 1 (bcs: 1/5). the isolate was typed as serotype 2b. mice experimentally infected with the clinical isolate of e. rhusiopathiae through subcutaneous or intraperitoneal routes survived, and the organism was recovered from t ...201120834197
symposium iii report: current status of veterinary vaccines.the 13th annual meeting of the japanese society of vaccinology convened the symposium on current status of veterinary vaccines on september 27, 2009 in sapporo, japan. a broad overview of veterinary vaccines from the past to the present and future as well as the latest developments such as erysipelothrix rhusiopathiae and mycoplasma hyopneumoniae for a platform vector for mucosa vaccine, inactivated avian influenza vaccine prepared from reassortant virus, and inactivated feline immunodeficiency ...201020888441
bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion.due to the environmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. however, little is known about the microbial risk present in sludge and its stabilized products. this study microbiologically explored the effects of anaerobic lagoon fermentation (alf) and autothermal thermophilic aerobic digestion (atad) on pathogen-related risk of raw swine manure by using culture-independent 16s rdna cloning and ...201020922382
immunization with truncated recombinant protein spac of erysipelothrix rhusiopathiae strain 715 serovar 18 confers protective immunity against challenge with various serovars.previously, we showed that surface protective antigen (spa) proteins of erysipelothrix rhusiopathiae can be classified into three molecular species-spaa, spab, and spac-and that spac is the most broadly cross-protective antigen among the three spa proteins. in this study, we examined the ability of the α-helical domain, which comprises the n-terminal half of spac, to elicit cross-protective immunity in mice and pigs. mice actively immunized with the full-length protein (rspac664) or the α-helica ...201020926696
oral use of penicillin in treatment of experimental erysipelothrix rhusiopathiae infection in mice. 194521010272
chemotherapy of erysipelothrix rhusiopathiae infections in mice. 194621024782
normal bacterial flora from vaginas of criollo limonero cows.in order to describe the normal bacterial flora in vaginas of criollo limonero cows, 51 healthy multiparous cows, at least 90-day postpartum, were selected. duplicated swabs (n = 102) were taken from the vaginal fornix of cows to perform aerobic and anaerobic cultures as well as conventional biochemical tests. out of 102 swabs, bacterial growth was obtained in 55 (53.9%) while the remaining 47 (46.1%) did not exhibited any bacterial growth. of the 55 bacterial growths, 23 (41.8%) were aerobic wh ...201021082249
histologic and bacteriologic findings in valvular endocarditis of slaughter-age pigs.endocarditis lesions from 117 slaughter pigs were examined pathologically and etiologically in addition to 90 control hearts with cardiac valves. lesions were located on the valves; however, the lesions had extended to the walls in 21 cases (18%). lesions predominated on the mitral valve (59%). a total of 28 cases, from which no growth was obtained or a contamination flora was grown, were screened by fluorescence in situ hybridization (fish) for bacteria (general bacterial probe) and probes spec ...201021088176
[mitral valve endocarditis after turkish "festival of sacrifice"]erysipelothrix rhusiopathiae is the causative agent of swine erysipelas. systemic infections caused by e. rhusiopathiae are rare, but often (90%) associated with endocarditis. in about 60% of cases endocarditis develops on normal heart valves, and despite appropriate antibiotic therapy about one-third of the patients requires valve replacement. we report the case of a housewife, who developed a mitral valve endocarditis due to e. rhusiopathiae after preparing meat for the turkish "festival of sa ...201021152883
characterization of erysipelothrix rhusiopathiae isolates from laying hens and poultry red mites (dermanyssus gallinae) from an outbreak of erysipelas.infection with the zoonotic bacterium erysipelothrix rhusiopathiae causes severe disease outbreaks (erysipelas) in poultry flocks. as this bacterium has been isolated from the poultry red mite (dermanyssus gallinae), this parasite has been suggested as a possible means of transmission of e. rhusiopathiae on and between poultry farms. to further elucidate the capacity of the mite as a reservoir, we analysed and compared 56 bacterial isolates from laying hens and nine isolates from mites by pulsed ...201021154061
endocarditis-associated brain lesions in slaughter pigs.left-sided valvular endocarditis (lsve) is a common finding in slaughter pigs. the lesion is often associated with renal thromboembolism, but information on embolization to other organs is sparse. this study focuses on the presence and type of endocarditis-associated brain lesions (eabls). the brains of 20 slaughter pigs with spontaneously arising lsve and 11 controls were examined by sectioning half of a formalin-fixed brain into 4mm slices for histological examination. the aetiology of the end ...201021168147
[microbial portrait. erysipelothrix rhusiopathiae]. 201021186506
erysipelothrix spp. genotypes, serotypes, and surface protective antigen types associated with abattoir condemnations.the objective of the current study was to investigate characteristics of erysipelothrix spp. from slaughter condemnations. specimens from 70 carcasses with lesions suspect for swine erysipelas were collected at an abattoir in iowa from october 2007 to february 2009. erysipelothrix spp. were isolated from 59 of 70 carcasses (84.3%). abattoir inspectors classified lesions as acute, subacute, or chronic; 8 of 8 (100%) were acute cases, 31 of 32 (96.9%) were subacute cases, and 20 of 30 (66.6%) were ...201121217046
causes of mortality in commercial organic layers in denmark.a longitudinal study investigated the courses of mortality in commercial free-range organic layer flocks in denmark. in total, 15 organic egg-producing flocks from 11 farms were randomly selected among 80 farms registered in denmark. four farms with confined egg production on deep litter were included for comparison. flock sizes ranged from 2260 to 5940 layers. the flocks were monitored from introduction to the layer farm until slaughter. flock mortalities ranged from approximately 2% to 91%, wi ...201021313846
acute meningitis as an initial manifestation of erysipelothrix rhusiopathiae endocarditis.erysipelothrix rhusiopathiae is a gram-positive bacillus which is found worldwide. although bloodstream infections caused by e. rhusiopathiae are not common, there is a strong association between bacteremia and the development of infective endocarditis. the risk of human infection with erysipelothrix is closely related to the opportunity for exposure to the organisms. we report a case of community-acquired meningitis as an initial manifestation of e. rhusiopathiae endocarditis in a 56-year-old w ...201121409531
the genome of erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, reveals new insights into the evolution of firmicutes and the organism's intracellular adaptations.erysipelothrix rhusiopathiae is a gram-positive bacterium that represents a new class, erysipelotrichia, in the phylum firmicutes. the organism is a facultative intracellular pathogen that causes swine erysipelas, as well as a variety of diseases in many animals. here, we report the first complete genome sequence analysis of a member of the class erysipelotrichia. the e. rhusiopathiae genome (1,787,941 bp) is one of the smallest genomes in the phylum firmicutes. phylogenetic analyses based on th ...201121478354
robust artificial intelligence tool for automatic start-up of the supplementary medium feeding in recombinant e. coli cultivations.one of the most important events in fed-batch fermentations is the definition of the moment to start the feeding. this paper presents a methodology for a rational selection of the architecture of an artificial intelligence (ai) system, based on a neural network committee (nnc), which identifies the end of the batch phase. the ai system was successfully used during high cell density cultivations of recombinant escherichia coli. the ai algorithm was validated for different systems, expressing thre ...201121479596
outbreak of swine erysipelas in a semi-intensive wild boar farm in spain.swine erysipelas (se) is a disease caused by the bacterium erysipelothrix rhusiopathiae and is one of the best-known and most serious diseases affecting domestic pigs. however, few studies exist concerning the susceptibility of wild boars to this disease and the role of this species as a reservoir. this study investigates and describes an outbreak of se that occurred on a semi-intensive wild boar breeding farm housing 40 boars in extremadura (sw spain) on 11-18 february 2010. seven animals died, ...201121649879
a retrospective study of post-mortem examination findings in takahe (porphyrio hochstetteri).to identify the major diseases or disease processes affecting both captive and wild populations of takahe (porphyrio hochstetteri) in birds submitted for post-mortem examination between 1992 and 2007, and to survey archived wild dead takahe for the presence of erysipelothrix rhusiopathiae.201121660844
soft tissue infections caused by marine bacterial pathogens: epidemiology, diagnosis, and management.skin and soft tissue infections (sstis) are one of the most common infection syndromes and may be caused by a large number of microorganisms. some principles of aquatic injuries are different than those of land-based trauma. wounds sustained in marine environment are exposed to a milieu of bacteria rarely encountered in different settings. these include vibrio spp., aeromonas spp., shewanella spp., erysipelothrix rhusiopathiae, mycobacterium marinum, streptococcus iniae, and other microbes. fail ...201121785929
infective endocarditis caused by erysipelothrix rhusiopathiae involving three native valves.human infection with erysipelothrix rhusiopathiae is extremely rare and occupationally related. this paper presents for the first time a case of a 47 year-old male with endocarditis involving three valves simultaneously.201121850630
outbreak of mortality in psittacine birds in a mixed species aviary associated with erysipelothrix rhusiopathiae infection.erysipelothrix rhusiopathiae septicemia, associated with an increased mortality of captive psittacines in a mixed-species aviary, was diagnosed by histopathology, gram staining, bacterial culture and sequencing, immunohistochemistry, and real-time polymerase chain reaction (pcr). over a period of 23 days with no premonitory signs, 2 rainbow lorikeets and an eclectus parrot died. of these birds, one lorikeet and the eclectus were submitted for necropsy. the main pathologic findings were thrombosi ...201121878682
characterization of erysipelothrix rhusiopathiae isolated from brazilian tayassu pecari.erysipelothrix rhusiopathiae causes a variety of diseases in many animal species, including human beings. most human infections caused by this pathogen are related to occupational exposure, and swine are considered to be the most important reservoir of e. rhusiopathiae. the white-lipped peccary (tayassu pecari) is an ungulate that has some genetic relationship to swine, and since the demand for t. pecari meat has recently increased in brazil and nothing is known about the relationship of this pe ...201121915617
deferment of slaughtering in swine affected by cutaneous erysipelas.according to the european union rules for meat inspection (regulation (ec) no. 854/2004 of the european parliament and of the council of 29 april 2004, that will be enforced in january 2006 in place of council directive 91/497/eec of 29 july 1991 and council directive 91/498/eec of 29 july 1991), any swine carcase affected by erysipelas must be destroyed. when pigs show erysipelas during antemortem inspection, the slaughtering must be deferred. legislation however does not specify how long shoul ...200622061544
erysipelothrix rhusiopathiae pneumonia in an immunocompetent patient.erysipelothrix rhusiopathiae is a gram-positive bacillus that causes infections primarily in animals. in humans, this organism usually causes localized cutaneous infections called erysipeloid. herein we report a case of pneumonia with isolation of e. rhusiopathiae from bronchoalveolar lavage and sputum. to our knowledge, this is the first report of pneumonia case caused by e. rhusiopathiae confirmed by culture.201122096132
Characterization of ß-galactoside phosphorylases with diverging acceptor specificities.Glycoside phosphorylases are a special group of carbohydrate-active enzymes, with characteristics in between those of glycoside hydrolases and glycosyl transferases. The phosphorylases from family GH-112 are exceptional because they employ galactose-1-phosphate instead of glucose-1-phosphate as glycosyl donor. Different acceptor specificities have been observed in this family, ranging from l-rhamnose to GlcNAc, GalNAc and a combination of the latter. Three new phosphorylases from previously unex ...201122112272
serological and pathogenic characterization of erysipelothrix rhusiopathiae isolates from two human cases of endocarditis in japan.we characterized the serological and pathogenic properties of two erysipelothrix rhusiopathiae isolates from human cases of infective endocarditis in japan. one isolate was recovered from a fisherman, and was identified as serovar 3, which is known to be prevalent among fish isolates. this strain exhibited high virulence in mice but was avirulent in swine. another was untypable, and avirulent in both mice and swine. our results suggest that various serological and athogenical types of e. rhusiop ...201122143815
seroprevalence evolution of selected pathogens in iberian wild boar.a total of 1279 eurasian wild boar (sus scrofa) sera were collected from 2000 to 2011 in the iberian peninsula to reveal time changes in serum antibody prevalences against selected infectious agents (porcine circovirus type 2, pcv2; porcine reproductive and respiratory syndrome virus, prrsv; hepatitis e virus, hev; and erysipelothrix rhusiopathiae) and to identify putative individual or population factors driving such changes. overall seroprevalences were 48%, 26%, 2% and 15% for pcv2, hev, prrs ...201122168900
erysipeloid (erysipelothrix rhusiopathiae infection) acquired from a dead kakapo. 201122184776
serological survey of exposure to erysipelothrix rhusiopathiae in poultry in new zealand.abstract aims: to estimate the seroprevalence of antibodies to erysipelothrix rhusiopathiae in chickens in new zealand, and to estimate the effect of housing type, geographical location and age on seroprevalence. methods: a cross-sectional serological survey of a convenience sample of 545 broiler, breeder, and layer chickens in 55 flocks was conducted in 2010-2011. birds were aged 5-83 weeks; housing types were free-range, shed, caged, and unknown; and flocks were located in the auckland, ma ...201122224872
[preparation and property study of the recombinant camel anti-spaa-n single domain antibodies].in order to obtain single domain antibody against surface protective antigen a (spaa)of erysipelothrix rhusiopathiae.201222230504
first case of erysipelas in a free-ranging bottlenose dolphin (tursiops truncatus) stranded in the mediterranean sea.an adult female bottlenose dolphin (tursiops truncatus) stranded alive and subsequently died several minutes later on the mediterranean coast of spain on 14 july 2010. clinical examination revealed foam through the blowhole and rales upon lung auscultation. on venipuncture, the blood was abnormally dense and dark. hematological and biochemical abnormalities included dehydration, leukocytosis (48 600 leukocytes microl(-1)) characterized by neutrophilia (48 200 neutrophils microl(-1)), and elevate ...201122303633
development of an enzyme-linked immunosorbent assay for the serological detection of exposure of poultry in new zealand to erysipelothrix rhusiopathiae and their serological response to vaccination.to modify and validate an existing swine erysipelas elisa for use with poultry serum and to assess the safety of a swine erysipelas vaccine for use in new zealand layer birds.201222352927
characterization of erysipelothrix rhusiopathiae strains isolated from recent swine erysipelas outbreaks in japan.the objective of the present study was to characterize erysipelothrix sp. strains from recent erysipelas outbreaks in japan. eighty-three (100%) strains were identified as e. rhusiopathiae, based on serotyping and spaa pcr. fifty (60.3%), 5 (6.0%), and 28 (33.7%) strains were isolated from animals with acute, subacute and chronic outbreaks, respectively, of which 79 (95.2%), 1 (1.2%), and 3 (3.6%) belonged to serotypes 1a, 2a, and untypeable, respectively. fifteen strains (including 3, 2, and 10 ...201222446396
effect of age and maternally-derived antibody status on humoral and cellular immune responses to vaccination of pigs against erysipelothrix rhusiopathiae.the effects of age and maternally-derived antibodies (mda) on the immune response to erysipelothrix rhusiopathiae were investigated in piglets orally vaccinated with a live e. rhusiopathiae vaccine at 6, 8 or 10 weeks of age. seroconversion, determined by elisa, was evident in mda positive piglets vaccinated at 8 or 10 weeks of age and in all mda negative vaccinates. two weeks after vaccination in the presence of mda, a t cell response, measured by a lymphocyte proliferation assay, was observed ...201222498786
successful medical treatment of erysipelothrix rhusiopathiae-induced lumbosacral diskospondylitis in a dog. 201222505240
empyema in spinal canal in thoracic region, abscesses in paravertebral space, spondylitis: in clinical course of zoonosis erysipelothrix rhusiopathiae.erysipelas is an animal disease caused by gram-positive bacteria erysipelothrix rhusiopathiae. among the domestic animals, domestic pig (sus scrofa f. domestica) suffers most frequently from the disease in human environment. this is a typical animal-borne disease observed mainly in occupational groups employed in agriculture, farming (of animals and birds), fishing and manufacturing industry.201222526696
collagen adhesin-nanoparticle interaction impairs adhesin's ligand binding mechanism.pathogenic bacteria specifically recognize extracellular matrix (ecm) molecules of the host (e.g. collagen, fibrinogen and fibronectin) through their surface proteins known as mscramms (microbial surface components recognizing adhesive matrix molecules) and initiate colonization. on implantation, biomaterials easily get coated with these ecm molecules and the mscramms mediate bacterial adherence to biomaterials. with the rapid rise in antibiotic resistance, designing alternative strategies to re ...201222538248
genetic variability in swine leukocyte antigen class ii and toll-like receptors affects immune responses to vaccination for bacterial infections in pigs.the genes encoding swine leukocyte antigen (sla) and toll-like receptor (tlr) are highly polymorphic in pig populations, and likely have influences on infection and the effects of vaccination. we explored the associations of different genotypes of sla class ii and of the genes tlr1, tlr4, tlr5, and tlr6 with antibody responses after vaccination against erysipelothrix rhusiopathiae (er) and actinobacillus pleuropneumoniae (app) serotypes 1, 2, and 5 in 191 duroc pigs maintained under specific pat ...201222658914
active infective endocarditis due to erysipelothrix rhusiopathiae: zoonosis caused by vancomycin-resistant gram-positive rod.a 42-year-old female who was a voluntary worker in a school for handicapped children was referred to us for surgery for active infective endocarditis. trans-esophageal echocardiography showed 2 large mobile vegetations on the aortic valve and severe aortic regurgitation. aortic valve replacement was performed to prevent septic embolism and deterioration of congestive heart failure. the empiric therapy with vancomycin, ampicillin, and gentamycin was initiated because a pathogen was not identified ...201322664947
evaluation of the andromas matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of aerobically growing gram-positive bacilli.matrix-associated laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is a rapid and simple microbial identification method. previous reports using the biotyper system suggested that this technique requires a preliminary extraction step to identify gram-positive rods (gprs), a technical issue that may limit the routine use of this technique to identify pathogenic gprs in the clinical setting. we tested the accuracy of the maldi-tof ms andromas strategy to identify a set o ...201222692743
cloning, auto-induction expression, and purification of rspaa swine erysipelas antigen.this work reports the cloning, expression, and purification of a 42-kda fragment of the spaa protein from erysipelothrix rhusiopathiae, the main antigenic candidate for a subunit vaccine against swine erysipelas. the use of an auto-induction protocol to improve heterologous protein expression in recombinant escherichia coli cultures was also investigated. the cellular growth pattern and metabolite formation were evaluated under different induction conditions. the his-tagged protein was over-expr ...201222729211
immunostimulatory effects of recombinant erysipelothrix rhusiopathiae expressing porcine interleukin-18 in mice and pigs.interleukin-18 (il-18), which was originally called gamma interferon (ifn-γ)-inducing factor, has been shown to play an important role in innate and acquired immune responses. in this study, attenuated erysipelothrix rhusiopathiae strains were engineered to produce porcine il-18 (poil-18) and evaluated for their potential immunostimulatory effect in animals. recombinant poil-18 was successfully expressed in the recombinant e. rhusiopathiae strains ys-1/il-18 and ko/il-18. the culture supernatant ...201222761300
development of a novel fluorescent microbead-based immunoassay and comparison with three enzyme-linked immunoassays for detection of anti-erysipelothrix spp. igg antibodies in pigs with known and unknown exposure.a novel fluorescent microbead immunoassay (fmia) using the recombinant polypeptide spaa415 was developed for detection of anti-erysipelothrix spp. igg in pig sera. the diagnostic performance of the fmia was evaluated on samples from pigs with known and unknown erysipelothrix spp. exposure and compared to an in-house enzyme-linked immunosorbent assay (elisa-1) based on the same capture antigen, and two commercially available elisas (elisa-2 and elisa-3). sera from pigs experimentally infected wit ...201222820200
thirty year retrospective evaluation of pneumonia in a bottlenose dolphin tursiops truncatus population.pneumonia is one of the most common causes of morbidity in bottlenose dolphins tursiops truncatus. to better understand associations of pneumonia with demographics, microbiology, pathology, and histopathology, a retrospective study on 42 dolphins from the us navy marine mammal program dolphin population was conducted (1980 to 2010). a total of 21 (50%) of the dolphins evaluated had pneumonia confirmed by histopathology. bacterial and fungal pneumonia was present in 42.9 and 28.6% of cases (9 and ...201222832722
capsular polysaccharide of erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, and its modification with phosphorylcholine.the capsule has been implicated in the virulence of the swine pathogen erysipelothrix rhusiopathiae, a rod-shaped, intracellular gram-positive bacterium that has a unique phylogenetic position in the phylum firmicutes and is a close relative of mollicutes (mycoplasma species). in this study, we analyzed the genetic locus and composition of the capsular polysaccharide (cps) of the fujisawa strain of e. rhusiopathiae. genome analysis of the fujisawa strain revealed that the genetic locus for capsu ...201222949554
cutaneous erysipelothrix rhusiopathiae (erysipeloid) infection in an immunocompromised child.erysipeloid, a cutaneous infection with the gram-positive bacillus erysipelothrix rhusiopathiae, is typically an occupational dermatosis seen in persons working with livestock or involved in commercial fishing (fishmongers). other more-generalized forms of infection with this organism also exist, including a septic form usually associated with endocarditis. many infections may be self-limited. they have rarely been reported in children or in immunocompromised patients. this microbe is sensitive ...201422957967
erysipelothrix rhusiopathiae isolates recovered from fish, a harbour seal (phoca vitulina) and the marine environment are capable of inducing characteristic cutaneous lesions in pigs.in order to determine the diversity and pathogenicity of erysipelothrix spp. isolates recovered from marine fish, a harbour seal (phoca vitulina) and the marine environment, 14 isolates were characterized by genotyping, serotyping, determination of the surface protective antigen (spa) gene type and assessment of virulence in a pig bioassay. all 14 isolates were erysipelothrix rhusiopathiae. isolates were determined to be of serotypes 2 (n = 3), 3 (n = 1), 4 (n = 1), 12 (n = 1), 15 (n = 1) or 21 ...201323083834
a case of apparent canine erysipeloid associated with erysipelothrix rhusiopathiae bacteraemia.erysipelothrix rhusiopathiae is a gram-positive facultative anaerobe found worldwide and is most commonly associated with skin disease in swine, while anecdotal reports of cases in dogs have been associated with endocarditis.201223140319
improving ante mortem diagnosis of erysipelothrix rhusiopathiae infection by use of oral fluids for bacterial, nucleic acid, and antibody detection.swine erysipelas is an economically important disease caused by erysipelothrix rhusiopathiae. pen-based collection of oral fluids has recently been utilized for monitoring infection dynamics in swine operations. the diagnostic performance of bacterial isolation, real-time pcr, and antibody detection by enzyme-linked immunosorbent assay (elisa) and fluorescent microbead-based immunoassay (fmia) methods were evaluated on pen-based oral fluid samples from pigs experimentally infected with e. rhusio ...201323201482
erysipelas in a numbat (myrmecobius fasciatus).an adult, female numbat (myrmecobius fasciatus) was submitted to the perth zoo veterinary department for postmortem examination in november 2011. this radio-collared wild numbat had been found dead in the dryandra woodland, 191 km southeast of perth, western australia. on external examination, the body condition was good. three ticks (ixodes spp.) were found on the thoracic region. the external pouch was contaminated with dirt and palpably flocculent, and the nipples oozed a purulent material. h ...201323505731
erysipelas in laying hens is associated with housing system.following the change from conventional cages to non-cage housing systems and furnished cages, which in sweden was finalised by 2005, problems caused by erysipelothrix rhusiopathiae increased in laying hen flocks. this study aimed to investigate possible associations between housing systems for laying hens and outbreaks of erysipelas. also, sera from 129 flocks in different housing systems, collected during 2005-2007, were analysed for the presence of antibodies to e rhusiopathiae using an indire ...201323542656
mitral regurgitation jet around neoannulus: mitral valve replacement in erysipelothrix rhusiopathiae endocarditis.a 50-year-old male presented with erysipelothrix rhusiopathiae (er) endocarditis of the mitral valve, severe mitral regurgitation, and heart failure. the er endocarditis destroyed the native mitral annulus therefore a new annulus was created for the suspension of the mitral bioprosthesis. postoperative neoannulus dehiscence and leak prompted to redo surgery where transesophageal echocardiography (tee) played an important role in pointing out the exact location of perineoannular leaks for repair.201623545868
something fishy: an unusual erysipelothrix rhusiopathiae infection in an immunocompromised individual.a 39-year-old man with a history of crohn's disease presented to the emergency department with a 12-h history of worsening febrile illness. he deteriorated rapidly with progression to sepsis and was admitted to the intensive care unit. initial treatment comprised broad spectrum antibiotics and inotropic support resulting in rapid improvement. with the immunocompromised state of the patient, and multiple blood cultures revealing erysipelothrix rhusiopathiae, a detailed history disclosed a recent ...201323559657
a child with erysipelothrix arthritis-beware of the little known.erysipelothrix rhusiopathiae is an established animal pathogen while the zoonotic infections in humans are rarely reported. infections occur after exposure to animals or animal products that are mostly occupational in adults. here we report in a child for the first time septic arthritis and osteomyelitis without an identifiable risk factor. a 5-year-old male child was admitted with pain in the left hip joint and inability to bear weight on the limb. clinical examination followed by radiological ...201223569959
erysipelothrix rhusiopathiae exploits cytokeratin 18-positive epithelial cells of porcine tonsillar crypts as an invasion gateway.tonsils are important organs for mucosal immunity and are gateways for various pathogens, including bacteria and viruses. the purpose of the present study was to reveal how erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, invades the mucosal epithelium of the tonsils of pigs. two germ-free piglets were orally infected with e. rhusiopathiae koganei 65-0.15, an attenuated vaccine strain in japan, and their tonsils of the soft palate were histologically examined four weeks aft ...201323601839
septicemia and aortic valve endocarditis due to erysipelothrix rhusiopathiae in a homeless man.we report a case of bacterial endocarditis due to erysipelothrix rhusiopathiae in a homeless man with no animal exposure. his course was complicated by an allergic reaction to ampicillin, urinary bladder infection, respiratory failure, and acute kidney injury. he recovered completely after aortic valve replacement and a 6-week course of intravenous ceftriaxone.201323662222
nonspecific erysipelothrix rhusiopathiae bacteremia in a patient with subclinical alcoholic liver disease.erysipelothrix rhusiopathiae, a pleomorphic gram-positive bacillus, is found widely in nature or as a commensal pathogen. it infects domestic animals such as swine, which may be the major reservoir of the organism. e. rhusiopathiae is primarily an occupational illness; 89% of the cases are linked to high-risk epidemiological situations. humans that are infected by this bacillus typically present with one or a combination of the following symptoms: localized skin lesion (erysipeloid), diffuse cut ...201323819078
non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in re. coli fed-batch cultures.in spite of the large number of reports on fed-batch cultivation of e. coli, alternative cultivation/induction strategies remain to be more deeply exploited. among these strategies, it could be mentioned the use of complex media with combination of different carbon sources, novel induction procedures and feed flow rate control matching the actual cell growth rate. here, four different carbon source combinations (glucose, glycerol, glucose + glycerol and auto-induction) in batch media formulation ...201323961396
erysipelothrix bacteremia without endocarditis: rare event or under-reported occurrence?a patient presented with inflamed hands and erysipelothrix rhusiopathiae bacteremia. because a high incidence of endocarditis has been reported with this organism, a transesophageal echocardiogram was obtained, which was normal. treatment with oral moxifloxacin resolved all manifestations of illness. the association between e. rhusiopathiae bacteremia and endocarditis may be spurious.201323988830
characterization and identification of a novel candidate vaccine protein through systematic analysis of extracellular proteins of erysipelothrix rhusiopathiae.erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, is a facultative intracellular gram-positive bacterium. it has been shown that animals immunized with a filtrate from e. rhusiopathiae cultures are protected against lethal challenge. in this study, we identified and characterized the extracellular proteins of e. rhusiopathiae to search for novel vaccine antigens. a concentrated culture supernatant from the e. rhusiopathiae fujisawa strain, which has been found to induce prot ...201324019408
prevalence of igy antibodies against erysipelothrix rhusiopathiae in a critically endangered parrot (kakapo, strigops habroptilus) and associated responses to vaccination.an enzyme-linked immunosorbent assay (elisa) was developed to estimate levels of igy antibody against the bacterium erysipelothrix rhusiopathiae in serum samples collected from the critically endangered kakapo (strigops habroptilus, psittaciformes, aves) before and after vaccination against this bacterium. relative igy antibody titres in pre-vaccination serum samples (n = 71 individual kakapo) were normally distributed with the exception of four outliers which displayed low igy levels. notably a ...201324066897
native valve endocarditis caused by erysipelothrix rhusiopathiae in an immunocompetent individual.infective endocarditis is a very rare clinical form caused by erysipelothrix rhusiopathiae. it is rarely seen in immunocompetent individuals. even after surgery it may entail mortality rates as high as 30-40 %. this report describes a case of native valve endocarditis caused by e. rhusiopathiae and cured with crystallized penicillin g and surgery.201324072768
development of a loop-mediated isothermal amplification assay for rapid and simple detection of erysipelothrix rhusiopathiae.erysipelothrix rhusiopathiae is a causative agent of swine erysipelas. we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for sensitive and rapid detection of e. rhusiopathiae. the lamp assay correctly detected 39 e. rhusiopathiae strains. no lamp products were detected from 14 non-rhusiopathiae erysipelothrix and 16 non-erysipelothrix strains, including e. tonsillarum serovar 10 strains, which are difficult to be discriminated from e. rhusiopathiae stra ...201424261887
prevalence of met-203 type spaa variant in erysipelothrix rhusiopathiae isolates and the efficacy of swine erysipelas vaccines in japan.since 2009, erysipelas infection among pigs in japan has been increasing. this study investigated the prevalence, and characteristics of erysipelothrix rhusiopathiae isolates in japan from 2008 to 2010 and assessed the efficacy of current commercial erysipelas vaccines. based on polymorphisms in a 432-bp hypervariable region in the surface protective antigen a (spaa) gene, 34 isolates were classified into three groups: (i) group 1 with methionine at position 203 (met-203) and isoleucine at posit ...201424405986
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