Publications

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the use of a wate-soluble carbodiimide as a coupling reagent in the passive hemagglutination test. 19664163006
detection of intracellular botulinum e toxin by fluorescent antibody technique. 19684178885
location of bacterial polysaccharide during various phases of growth.polysaccharide-containing material was located by histochemical staining on ultrathin sections of bacillus cereus and several clostridial species during various phases of growth. these components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. in b. cereus any carbohydrate-containing material present along the forespore membrane was too small to be detected by direct staining. however, in three clostridial species such material was ...19694182530
[colorimetric method of determination of polysaccharides in bacterial cells]. 19674183519
[spectral characteristic of the products of biuret reaction as an indicator in the study of the metabolism of microorganisms]. 19684184037
[role of axoplasm synthesis disorders in the nerve cell in lesions of the peripheral neuromuscular apparatus induced by botulinum toxin]. 19664194255
[the isoelectric point of extracellular proteases of certain bacterial species]. 19724200183
proteases of clostridium botulinum. iv. inhibitors against proteases from clostridium botulinum. 19734202008
a 'new' saliva substance, probably inherited, and serologically independent of abh, lewis, and sda blood group substances. 19734204386
direct enzymatic repair of deoxyribonucleic acid single-strand breaks in dormant spores.with the alkaline sucrose gradient centrifugation method, it was found that dormant spores of clostridium botulinum subjected to 300 krads of gamma radiation showed a distinct decrease in deoxyribonucleic acid (dna) fragment size, indicating induction of single-strand breaks (ssb). a two- to threefold difference in radiation resistance of spores of two strains of c. botulinum, 33a (37% survival dose [d(37)] = 110 krads) and 51b (d(37) = 47 krads), was accompanied by relatively larger dna fragmen ...19744206867
[alteration of the fractional composition and antigenic structure of botulin toxin e in the process of detoxication]. 19734207091
[use of the fluorescent antibody method for microbiological control in the food-processing industry (review)]. 19724207751
rapid detection of clostridium botulinum toxin by capillary tube diffusion.a micro capillary agar-gel diffusion system for the detection of botulinal toxin in foods and cultures was developed and evaluated. toxins types a, b, and e, produced in culture broth with and without added trypsin, and type e toxin, produced in inoculated canned clams, were tested with this system and with the mouse bioassay procedure. with nontrypsinized toxin, the capillary diffusion system detected as little as 100 minimal lethal doses (mld) per ml but was effective only at higher levels, 10 ...19744208636
[possibilities of combined vaccination of minks against aujeszk's disease, distemper and botulism]. 19724210743
isolation of a common cell wall antigen from the proteolytic strains of clostridium botulinum. 19744212113
purification and some properties of progenitor toxins of clostridium botulinum type b.purification of progenitor toxin of clostridium botulinum type b strain okra was undertaken by sequential steps of acid precipitation, extraction, ammonium sulfate precipitation, ribonuclease digestion, acid precipitation, protamine treatment, sulphopropyl-sephadex chromatography, and sephadex g-200 gel filtration. two different molecular-sized toxins, named large (l) and medium (m) toxins, were obtained. l toxin was centrifugally homogeneous but electrophoretically heterogeneous. it contained 2 ...19744214781
[preparative separation of toxin and prototoxin from cl. botulinum type b cells]. 19744218008
[distribution of cl. botulinum in the soil of the shores of lake balkhash]. 19694245029
[distribution of clostridium botulinum in mendoza, argentina]. 19704250723
antigen and antibody in aleutian disease in mink. ii. the reaction of antibody with the aleutian disease agent using immunodiffusion and immunoelectroosmophoresis.aleutian disease viral (adv) antigen was prepared by fluorocarbon extraction of spleen, liver, and lymph nodes from mink experimentally infected ten days previously. using a potent adv antigen, antibody was detected by immunodiffusion (id) and immunoelectroosmophoresis (ieop). utilizing these precipitin tests, antibody was detected in all the mink sera tested as early as seven days after experimental infection. titer of antibody increased throughout the infection period. titers of more than 100 ...19734270428
125i-labeled neurotoxin from clostridium botulinum a: preparation, binding to synaptosomes and ascent to the spinal cord. 19744273827
antagonistic effect on clostridium botulinum type e by organisms resembling it.a bacteriocin-like substance, active against strains of clostridium botulinum type e, is produced by certain nontoxic organisms whose biochemical properties and morphological characteristics are similar to type e. the substance, for which the name "boticin e" is proposed, is bacteriolytic for vegetative cells and bacteriostatic for spores of type e. its spectrum of activity is somewhat strain-specific. of the clostridial species tested, only c. botulinum type e and, to a lesser extent, c. perfri ...19664288828
agar concentration in counting clostridium colonies.decreasing the agar concentration of a counting medium from the usual 1.5% resulted in larger colonies with less interference from gas in clostridium botulinum 115b and c. sporogenes pa 3679. optimal agar concentration was 0.65% for c. botulinum with 24-hr incubation and 0.50% for c. sporogenes with 48-hr incubation. lower concentrations yielded growth too diffuse for counting. motility was considered the explanation for increased colony size in softer agar. the greater the degree of motility, t ...19674291671
experimental production of diarrhea and its prevention by malethamer in monkeys. 19674293386
sporulation of mesophilic clostridia. 19674295013
low temperature growth characteristics of clostridia. 19684296971
the effects of botulinum toxin on the pattern of innervation of skeletal muscle in the mouse. 19684297234
partial antagonism of experimental botulinal intoxication. 19674297676
drugs affecting transmitter release from motor nerve terminals. 19674297759
culture isolation and identification of clostridia. 19674300051
proline as an intermediate in the reductive deamination of ornithine to delta-aminovaleric acid.fresh extracts of cells of clostridium botulinum reduced a limited amount of ornithine to delta-aminovaleric acid, but at high substrate concentrations a considerable amount of an amino compound accumulated which was neutral at ph 4.2. aging of the extracts at -10 c or freezing and thawing resulted in the loss of the ability to produce delta-aminovaleric acid, but the ability to produce the neutral compound was retained. this compound was separated by column chromatography, and was found to be i ...19684301045
collagenolytic activity of bacteria.actively growing aerobic and anaerobic bacteria were screened by a plate assay, with reconstituted guinea pig collagen as a substrate, for their ability to produce a collagenolytic factor. collagenolytic activity was not demonstrated among the aerobic organisms tested, with the exception of one strain of staphylococcus aureus (only when grown under anaerobic conditions). collagenolytic activity, however, was detected in cultures of clostridium tetani and bacteroides species other than b. melanin ...19694306539
[on the localization of ag and lp by means of density gradient ultracentrifugation]. 19694306550
effect of botulinum toxin on speed of skeletal muscle contraction. 19694306768
inhibition of protease production of various bacteria by ammonium salts: its effect on toxin production and virulence.production of protease by many bacteria was found to be inhibited by ammonium salts, and the enzyme production was more sensitive to the salts than was growth of the organisms. inhibition of protease production by some pathogenic bacteria may result in the recognition of an exotoxin which otherwise would have been digested by the protease. in the case of pseudomonas aeruginosa, qualitatively different toxicities could be demonstrated in the culture fluids, depending on the presence or absence of ...19694309097
neomycin-containing media in the isolation of clostridium botulinum and food poisoning strains of clostridium welchii. 19694309920
reactions involved in the conversion of ornithine to proline in clostridia.an enzyme system which converts ornithine to proline was partially purified from extracts of cells of clostridium botulinum and of clostridium pa 3670 by fractionation with ammonium sulfate and by dialysis in the presence of 0.01 m ornithine. nicotinamide adenine dinucleotide (nad) was the only cofactor required for maximal activity of the partially purified system. a possible intermediate in the conversion was accumulated when a high concentration of proline was used as substrate and the nad wa ...19694311194
gas chromatographic analysis of amines and other compounds produced by several species of clostridium. 19694312933
a study of the effect of ionizing radiation on resistance, germination, and toxin synthesis of clostridium botulinum spores, types a, b, and e. coo-1095-3. 19664312998
microbiological aspects of radiopasteurized chicken. nyo-3733-4. 19694313242
microbiological aspects of radiopasteurized chicken. nyo-3733-7. 19694313243
microbiological aspects of radiopasteurized chicken. nyo-3733-6. 19694313244
microbiological aspects of radiopasteurized chicken. nyo-3733-5. 19694313245
microbiological aspects of radiopasteurized chicken. nyo-3733-3. 19694313246
[symposium: welchii and botulinum bacilli]. 19694314558
the pathogenic actions of exotoxins. 19704315443
[a medium for the differentiation of some pathogenic clostridia]. 19684317009
characterization of clostridia by gas chromatography differentiation of species by trimethylsilyl derivatives of whole-cell hydrolysates.trimethylsilyl (tms) derivatives prepared from whole-cell hydrolysates of 36 strains, representing 10 species of clostridium were examined by gas-liquid chromatography (glc). the tms profile of each species contained a group of peaks which characterized the species. variation among strains within a species was much lower than variation between species. some of the closely related clostridia could be differentiated by comparing their tms profiles. strains of clostridium botulinum were distinguish ...19704318575
[nature of the epr spectrum of concentrated diphtheria toxins and antitoxins]. 19694319324
clostridia isolated from human mouths. 19704319864
[action of the amino acids plus sulfite combination on the growth of clostridium. trypticase-glucose-tyrosine-sulfite (tgts) medium]. 19704320222
incidence of the food poisoning clostridia in meat animals. 19674322513
tolerance of bacteria to high concentrations of nacl and glycerol in the growth medium.when compared at similar levels of water activity, glycerol was more inhibitory than sodium chloride to relatively salt-tolerant bacteria and less inhibitory than salt to salt-sensitive species.19714322678
fine structure of protoplasts and l-forms of clostridium botulinum types a and e.spherical bodies, that are obtained by adding penicillin and lysozyme to clostridium botulinum types e and a cultures which are growing in an osmotically stabilized medium, are shown to be protoplasts by electron microscopy. the l-forms of these two culture types have morphologically different inclusion bodies.19714323294
analysis of clostridium botulinum toxigenic types a, b, and e for fatty and carbohydrate content.lyophilized, 48-hr log-phase vegetative cells were extracted with chloroform-methanol (2:1, v/v) and ethanol-ether (3:1, v/v) and then saponified with methanolic koh. gas-liquid chromatography of the methyl esters of extractable fatty acids revealed distinctive "pattern profiles" of clostridium botulinum toxigenic types "a," "b," and "e." c. perfringens type "a" and escherichia coli strain "b" were also studied in a similar manner and were found to give pattern profiles which were distinct even ...19714324194
symposium on microbial changes in foods. factors affecting the production of bacterial food poisoning toxins. 19714327570
the effect of type d botulinum toxin on frog neuromuscular junctions.1. botulinum toxin type d blocks neuromuscular transmission in frogs. motor nerve impulses continue to invade the nerve terminals but cease to evoke the phasic release of transmitter normally associated with them.2. sensory receptors in the muscle continue to generate impulses even after 4 days of continuous exposure to botulinum toxin.3. contrary to expectations, spontaneous miniature end-plate potentials did not disappear completely after botulinum intoxication; they still occurred, although w ...19714329008
ganglioside inactivation of botulinum toxin. 19714330291
clostridium perfringens. i. sporulation in a biphasic glucose-ion-exchange resin medium.a biphasic culture medium suitable for cultivation and sporulation of clostridium perfringens, c. botulinum, and c. sporogenes was devised. the medium designed for use in a disposable, compartmented, plastic film container contained peptones, yeast extract, minerals, an anion exchange resin, and glucose in 4% agar as the solid phase and (nh(4))(2)so(4) and 0.1% agar as the liquid phase. with the biphasic system, it was not necessary to use active cultures as inocula. growth was at least equal to ...19714332043
[botulism]. 19724338407
bacterial contamination of food. 19724340202
[lipase and tweenase activity of cl. perfringens and cl. botulinum]. 19724344071
[circulating toxin, an aid in the diagnosis of type b botulism in man]. 19734353197
neuromuscular physiology of wound botulism. 19734357216
proteases of clostridium botulinum. v. studies on the serological relationship between proteases from clostridium botulinum and other spore-forming bacteria. 19734359093
report on microbiological methods. 19744361305
mesophilic clostridia in puget sound. 19744362754
toxic proteins produced by clostridium botulinum. 19744365698
complementation in theta-toxin production between mutants of two groups of clostridium perfringens. 19744366423
effect of potassium sorbate on salmonellae, staphylococcus aureus, clostridium perfringens, and clostridium botulinum in cooked, uncured sausage.skinless precooked, uncured sausage links with and without potassium sorbate (0.1% wt/wt) were inoculated with salmonellae, staphylococcus aureus, clostridium perfringens, and clostridium botulinum and held at 27 c to represent temperature abuse of the product. total counts of uninoculated product showed that the normal spoilage flora was delayed 1 day when sorbate was present. growth of salmonellae was markedly retarded by sorbate. growth of s. aureus was delayed 1 day in the presence of sorbat ...19744368631
incidence of clostridia in meat products. 19744368805
[results of an inquiry into the types of packed meat and their microbiological requirements]. 19734369665
an intracellular alpha-d-glucan from clostridium botulinum, type e. 19724369776
[recent developments in the diagnosis of bacterial food intoxications (author's transl)]. 19744376320
[botulin intoxication in the child]. 19704397428
urinalysis as an aid in the field diagnosis of clostridium botulinum intoxication in cattle. 19734490302
comparative studies of an asporogenic mutant and a wild type strain of clostridium botulinum type e 1 . 19724551615
thermal stability of the deoxyribonucleic acid hybrids between the proteolytic strains of clostridium botulinum and clostridium sporogenes. 19724551616
fixation of mature spores of clostridium botulinum.a triple fixation method using a sequential application of 15 or 30% formaldehyde, 6% glutaraldehyde, and 1% osmium tetroxide resulted in excellent fixation of mature spores of clostridium botulinum.19724551752
survival and dormancy of clostridia spores. 19724552272
[botulism in a mink ranch in quebec]. 19724552746
possible origin of clostridium botulinum contamination of eskimo foods in northwestern alaska.soils from beaches in northwestern alaska have been found to contain clostridium botulinum type e, providing evidence of one environmental source of food contamination.19724552895
[ultrastructure of the cell wall of cl. botulinum type a]. 19724553062
interrelationship of heat and relative humidity in the destruction of clostridium botulinum type e spores on whitefish chubs.heat destruction of types b and e clostridium botulinum spores on whitefish chubs was observed to be dependent upon the relative humidity (rh) in the chamber in which fish were heated. experimental conditions were designed to simulate those attainable in commercial fish-smoking plants. low numbers of type e spores were destroyed with regularity, within 30 min, on fish which were held at an internal temperature of 77 c (170.6 f) in an atmosphere of at least 70% rh. however, an internal temperatur ...19724553143
the role of intracellular glucan in endogenous fermentation and spore maturation in clostridium botulinum type e. 19724553163
bacteriophage and the toxigenicity of clostridium botulinum type d. 19724553461
[growth of cl. botulinum type b in preserved mushroom sauce]. 19724554867
[effect of increased chloride on the spontaneous release of acetylcholine in the neuromuscular synapses of amphibia poisoned with clostridium toxin type a]. 19724555317
distribution of clostridium botulinum type e in the gulf of st. lawrence in relation to the physical environment. 19724556099
isolation and taxonomic significance of bacteriophages for non-proteolytic clostridium botulinum. 19724556256
isolation and characterization of a protease from clostridium botulinum type b. 19724556539
[biological method of metronidazole and dimetridazole determination on anaerobic germs]. 19724558580
botulism in birds and mammals in great britain.. 19724558592
activation of a toxic component of clostridium botulinum types c and d by trypsin.when the stockholm and 468c strains of type c and the 1873 strain of type d clostridium botulinum are "cured" of their prophages, they simultaneously discontinue the production of their dominant toxins (c(1) and d), but they continue to produce a second antigenically monospecific toxin (c(2)). these "cured" strains of types c and d therefore become indistinguishable with respect to the toxin produced. fifteen type c cultures received from other laboratories discontinued to produce the dominant t ...19724560464
clostridium botulinum type f: isolation from venison jerky.a clostridium botulinum type f was isolated from the venison jerky responsible for the only type f botulism outbreak reported in the united states. the isolate differed from the prototype langeland type f strain in being nonproteolytic.19724561099
exosporium formation in sporulating cells of clostridium botulinum 78a.in sporulating cells of clostridium botulinum type a (nca strain 78a), formation of exosporia was initiated laterally in the sporangia before the synthesis of the spore cortex. mature spores were enveloped by multilayered exosporia; the layers were uniform in appearance, approximately 3 nm thick, with a center-to-center distance of 7 nm.19724562412
radiation sterilization of prototype military foods: low-temperature irradiation of codfish cake, corned beef, and pork sausage."screening" packs comprising 10 lots each of codfish cake, corned beef, and pork sausage, each lot containing about 10(6) spores of a different strain (five type a and five type b) of clostridium botulinum per can, were irradiated at -30 +/- 10 c with a series of increasing doses (20 replicate cans/dose) of (60)co gamma rays. the cans were incubated for 3 months at 30 c and examined for swelling, toxin, and recoverable botulinal cells. based on the latter criterion of spoilage, median lethal dos ...19724562483
[identification of toxin and isolation of the strain in a case of botulism]. 19724562725
variability in ultrastructure of clostridium botulinum spores. 19724563855
radiometric detection of some food-borne bacteria.studies on detection of bacteria by radiometric techniques have been concerned primarily with aerobic species in clinical specimens. the data presented here are related to detection of aerobic and anaerobic species that are of significance in foods, by measurement of (14)co(2) evolved from the metabolism of (14)c-glucose. salmonella typhimurium and staphylococcus aureus were inoculated into tryptic soy broth containing 0.0139 muci of (14)c glucose/ml of medium. detection times ranged from 10 to ...19724564040
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