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glutamine synthetase of bacillus stearothermophilus. ii. regulation and thermostability. 19744152245
purification and properties of glutamine synthetase from bacillus stearothermophilus. 19744152611
conformational change with temperature and thermostability of glutamine synthetase from bacillus stearothermophilus. 19744153301
[dissociation of 2 enzymatic activities from isoleucyl-ribonucleic acid synthetase of "bacillus stearothermophilus"]. 19664165990
the effect of temperature on the glucose utilization by bacillus stearothermophilus. 19674166066
[the differential effect of heat on two enzymatic activities of isoleucyl-acid ribonucleic-synthetase of "bacillus stearothermophilus"]. 19674167023
[comparison of the isoleucyl, leucyl- and valyl-srna synthetases of "escherichia coli" and "bacillus stearothermophilus". ii. stability of the complexes (aminoacyl-adenylate-enzyme)]. 19684175042
[on the thermal inactivation of isoleucyl-trna synthetase of "bacillus stearothermophilus". rectification]. 19684175051
[comparison of the isoleyl- and leucyl-trna synthetases of "bacillus stearothermophilus" and "escherichia coli". 1. properties of the enzymes]. 19684175052
[effects of trna on thermal denaturation of escherichia coli and bacillus stearothermophilus leucyl-trna synthetases]. 19684184438
[purification and some properties of bacillus stearothermophilus leucyl-trna synthetase]. 19694186386
functional correspondence between 30s ribosomal proteins of escherichia coli and bacillus stearothermophilus.30s ribosomal proteins from bacillus stearothermophilus (b. proteins) have been fractionated and characterized with respect to their ability to replace various e. coli 30s proteins (e. proteins) in the e. coli 30s ribosome reconstitution system. the functional counterparts of all the e. proteins, except s1, s6, s9, and s13, have been tested. in all cases, b. proteins can substitute for e. proteins. several purified b. proteins are chemically different from their functionally homologous e. protei ...19734197630
additional antibiotic inhibitors of peptidoglycan synthesis.diumycin, janiemycin, nisin, and subtilin inhibited peptidoglycan synthesis catalyzed by particulate enzyme systems from bacillus stearothermophilus and escherichia coli. all of these, except for nisin, also induced accumulation of the lipid intermediate in peptidoglycan synthesis. concentrations required for 50% inhibition of peptidoglycan synthesis were less than 0.1 mug/ml for diumycin and in the range of 10 to 100 mug/ml for janiemycin, nisin, and subtilin in both organisms. the discrepancy ...19734202341
separation of transfer rna's from bacillus subtilis, bacillus stearothermophilus and chicken liver by polyacrylamide gel electrophoresis. 19744209781
purification and characterization of the thermophilic d-alanine carboxypeptidase from membranes of bacillus stearothermophilus. 19744211099
binding of (14c)penicillin g to the membrane-bound and the purified d-alanine carboxypeptidases from bacillus stearothermophilus and bacillus subtilis and its release. 19744214007
characterization of a bacteriophage-induced, host-specific lytic enzyme from lysates of bacillus stearothermophilus infected with bacteriophage tp-8.phage tp-8 lysates of bacillus stearothermophilus 4s or 4s(8) contain lytic activity exhibiting two ph optima, one at ph 6.5 and the other at ph 7.5. using a variety of fractionation procedures, the two lytic activities could not be separated. at ph 7.5 the lytic enzyme is an endopeptidase which hydrolyzes the l-alanyl-d-glutamyl linkage in the peptide subunits of the cell wall peptidoglycan and at ph 6.5 it exhibits n-acetylmuramidase activity. endopeptidase activity is inhibited by nacl and ne ...19744218232
autolysis of cell walls of bacillus stearothermophilus b65 and the chemical structure of the peptidoglycan.1. the cell walls of bacillus stearothermophilus b65 contain glucosamine, muramic acid, alanine, alphain-diaminopimelic acid (dap), glutamic acid, aspartic acid, glycine, and serine in the molecular proportions 0.60:0.64:2.30:0.85:1.00:0.11:0.13:0.31. 2. both d- and l-alanine are present, but glutamic acid and diaminopimelic acid are present only as the d- and meso-isomers respectively. 3. the peptide fragments ala-dap, dap-ala, and dap-ala-dap have been isolated from a partial acid hydrolysate ...19704248963
peptidoglycan synthesis in bacilli. i. effect of temperature on the in vitro system from bacillus megaterium and bacillus stearothermophilus. 19714255188
structure of the cell wall of bacillus stearothermophiluys: mode of action of a thermophilic bacteriophage lytic enzyme.the mode of action of a bacteriophage lytic enzyme on cell walls of bacillus stearothermophilus (nca 1503-4r) has been investigated. the enzyme is an endopeptidase which catalyzes the hydrolysis of the l-alanyl-d-glutamyl linkage in peptide subunits of the cell wall peptidoglycan. preliminary studies on the soluble components in lytic cell wall digests indicate that the glycan moiety is composed of alternating glucosamine and muramic acid; one half of the muramic acid residues contain the tripep ...19714255338
[bacillus stearothermophilus strain growing on an agarized mineral medium with liquid paraffin]. 19714256891
in search of lactate dehydrogenase from bacillus stearothermophilus. 19714257057
preliminary separation of enzymes extracted from bacillus stearothermophilus. 19724263100
nitrate reductase and respiratory adaptation in bacillus stearothermophilus.downey, r. j. (university of notre dame, notre dame, ind.). nitrate reductase and respiratory adaptation in bacillus stearothermophilus. j. bacteriol. 91:634-641. 1966.-bacillus stearothermophilus 2184 required nitrate to grow in the absence of oxygen. like many facultative microorganisms, the growth obtained anaerobically was considerably less than that obtained aerobically, even though the dissimilatory reduction of nitrate is, in effect, anaerobic respiration. the ability to reduce nitrate de ...19664286885
the glycerol teichoic acid from the cell wall of bacillus stearothermophilus b65.1. a glycerol teichoic acid has been extracted from cell walls of bacillus stearothermophilus b65 and its structure examined. 2. trichloroacetic acid-extractable teichoic acid accounted for 68% of the total cell-wall phosphorus and residual material could be hydrolysed to a mixture of products including those characteristic of glycerol teichoic acids. 3. the extracted polymer is composed of glycerol, phosphoric acid, d-glucose and d-alanine. 4. hydrolysis of the polymer with alkali gave glycerol ...19664290549
some chemical and physical properties of thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. 19674291833
the amino acid composition and other properties of thermostable glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. 19694304231
homology of ribosomal ribonucleic acid diverse bacterial species with escherichia coli and bacillus stearothermophilus.hybridization competition experiments were used to examine the ribosomal ribonucleic acid (rrna) homologies of 22 bacteria and 3 higher organisms with escherichia coli and bacillus stearothermophilus. although little or no homology was observed with the higher organisms, the bacteria showed a wide range of homologies. organisms whose rrna showed closer homology to e. coli rrna showed less rrna homology to b. stearothermophilus rrna and vice versa.19714329734
localization and solubilization of the respiratory nitrate reductase of bacillus stearothermophilus.membranes were isolated from bacillus stearothermophilus 2184d by lysozyme digestion of the cell wall and subsequent differential centrifugation. observations with the electron microscope indicate that such membranes are relatively intact and have a typical membrane appearance. nitrate will preferentially oxidize the cytochrome b of such membranes. approximately 80% of the total respiratory nitrate reductase activity of whole cells can be localized in the washed membrane fraction and the process ...19724333609
physical aggregation and functional reconstitution of solubilized membranes of bacillus stearothermophilus.isolated membranes of bacillus stearothermophilus 2184d can be disrupted by treatment with sodium dodecyl sulfate (sds). this disruption is attended by a decreased turbidity of membrane suspensions and a differential loss of activities of the electron transport system. reduced methyl viologen (mvh)-nitrate reductase activity is insensitive to sds treatment, whereas reduced nicotinamide adenine dinucleotide (nadh)-nitrate reductase and cyanide-sensitive nadh oxidase activities are decreased by 80 ...19724333610
isoleucyl-transfer ribonucleic acid synthetase from bacillus stearothermophilus. i. properties of the enzyme. 19724336851
isoleucyl-transfer ribonucleic acid synthetase from bacillus stearothermophilus. 2. dynamics of the enzyme-substrate interactions. 19724336852
thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. i. immunochemical studies. 19724338311
arginyl-transfer ribonucleic-acid synthetase from bacillus stearothermophilus. purification, properties and mechanism of action. 19724351436
isolation of different thermophilic enzymes from bacillus stearothermophilus. 19734354752
atpase and gtpase activities associated with a specific 5s rna-protein complex.an rna-protein complex consisting of 5s rna and two ribosomal proteins, b-l5 and b-l22, was isolated from bacillus stearothermophilus ribosomes and found to be active in gtp hydrolysis. this activity was not influenced by elongation factor g. further analysis of this complex showed that it was also able to hydrolyze atp. inhibition studies revealed that atp was a noncompetitive inhibitor of gtp and that gtp was also a noncompetitive inhibitor of atp, indicating that two different enzymatic sites ...19734355369
glyceraldehyde 3-phosphate dehydrogenase of bacillus stearothermophilus. kinetics and physicochemical studies. 19734358785
a homogeneous, thermostable deoxythymidine kinase from bacillus stearothermophilus. 19744371810
correlation between thermal death and membrane fluidity in bacillus stearothermophilus.paramagnetic resonance spectra of spin labels partitioned into spheroplast membranes of bacillus stearothermophilus indicate lateral lipid phase separations. cells adjust their lipid composition in response to temperature changes so that the same change of state in membrane phospholipids is achieved at the respective growth temperature. a temperature-sensitive mutant that fails to change its lipid composition above a certain temperature can survive only up to the higher temperature boundary for ...19744372606
thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus: ii. kinetic and immunokinetic analysis. 19744376605
the effect of yeast cells in the heating medium on the heat resistance of bacillus stearothermophilus spores. 19654380442
activation of bacillus stearothermophilus spores and release of dipicolinic acid after hydrochloric acid treatment. 19684384158
the effect of storage conditions on the heat resistance and heat activation response of bacillus stearothermophilus spores. 19684386550
isolation and some properties of the triphosphopyridine nucleotide isocitrate dehydrogenase from bacillus stearothermophilus. 19704393512
properties and regulation of pyruvate carboxylase from bacillus stearothermophilus. 19704394197
purification and metal ion activation of an aminopeptidase (aminopeptidase ii) from bacillus stearothermophilus. 19744407342
bacillus stearothermophilus in soils of iceland.bacillus stearothermophilus was found in icelandic soils. of the 33 strains studied, only four grew at 25 c. temperatures as high as 27 c in bare soil at a depth of 1 to 1.5 cm were measured at the collection site. however, to demonstrate growth the cultures had to be incubated for 3 weeks, and soil temperatures remained at 27 c only for shorter periods of time.19744421871
altered 30s ribosomal proteins in streptomycin resistant, dependent and independent mutants of bacillus stearothermophilus. 19744427590
teichoic acid synthesis in bacillus stearothermophilus.1. particulate enzyme preparations obtained from bacillus stearothermophilus b65 by digestion with lysozyme were shown to catalyse teichoic acid synthesis. with cdp-glycerol as sole substrate the preparations synthesized 1,3-poly(glycerol phosphate). it was characterized by alkaline hydrolysis, by glucosylation to the alkali-stable 2-glucosyl-1,3-poly(glycerol phosphate) with excess of udp-glucose and a bacillus subtilis marburg enzyme system, by degradation of this latter product with 60%hf and ...19744429546
production of a new thermostable neutral alpha-galactosidase from a strain of bacillus stearothermophilus. 19744429799
phosphoglycerate kinase of bacillus stearothermophilus. 19744436288
relationship of soil constituents to spore counts and heat resistance of bacillus stearothermophilus. 19744441977
thermophilic aminopeptidase from bacillus stearothermophilus. iv. 19744455642
[heat resistance of bacillus stearothermophilus spores depending on their dipicolinic acid and calcium concentration]. 19744458258
the thermal resistance of bacillus stearothermophilus spores. the effects of temperature and ph of the heating medium. 19744462138
[heat resistance of dryed bacillus stearothermophilus spores during storage at different temperatures and different relative humidities (author's transl)]. 19744479585
the function of the two subunits of thermophilic aminopeptidase i.the thermophilic high-molecular-weight aminopeptidase i (ec 3.4.11.1) from bacillus stearothermophilus is composed of 12 subunits of two different types (alpha,beta) which can combine in various ratios. only one type of subunit (alpha) is needed for the degradation of neutral peptides, but dipeptides having amino-terminal aspartic or gultamic acid are substantially hydrolyzed only by enzyme containing the other subunit (beta) as well. asp-gly inhibits the enzymatic hydrolysis of glutamic acid 1- ...19734521203
studies on variants of bacillus stearothermophilus strain nca 1518.the heat resistance, fermentation reactions, nutritional requirements, and phage sensitivity of 18 selected morphological variants of bacillus stearothermophilus nca 1518 were studied. it was found that when smooth variants mutated to rough colonial morphology, there was no concurrent change in fermentation reactions, nutritional requirements, or heat resistance. the smooth variant, and the rough mutants derived directly from it, presented a uniform pattern of biochemical capabilities which diff ...19724553138
identification of named cultures of bacillus calidolactis as bacillus stearothermophilus.a study was made of 23 cultures of bacillus calidolactis. by using cultural, biochemical, and per cent guanine plus cytosine methods, the cultures were found to be b. stearothermophilus.19724553846
sporulation and heat resistance of bacillus stearothermophilus spores produced in chemically defined media.the effect of amino acids on sporulation is discussed. heat-resistant spores were produced in a chemically defined medium.19724553847
hazard inherent in microbial tracers: reduction of risk by the use of bacillus stearothermophilus spores in aerobiology.the use of a "biological tracer" forms an essential part of many aerobiological experiments. where release of such tracers is likely to result in deliberate or inadvertent human exposure, safety becomes a primary consideration in the selection of the tracer organism. of the three most commonly used organisms, namely bacillus subtilis, escherichia coli, and serratia marcescens, only the first comes near to satisfying the need for nonpathogenicity and even it has been incriminated as a cause of hu ...19724557557
studies on a thermophilic bacillus: its isolation, properties, and temperature coefficient of growth.a thermophilic bacillus with minimal, optimal, and maximal growth temperatures of 40, 64.5, and 72 c, respectively, was isolated from soil. biochemical and morphological studies place the isolate in group 1 of the classification of walker and wolf. after adaption to nitrate broth, the temperature coefficient for growth was found to be 20,400 cal/mol. when the temperature coefficient for growth of the isolate, psychrophilic bacteria, mesophilic bacteria, and a strain of bacillus stearothermophilu ...19724565524
isolation and characterization of 5s rna-protein complexes from bacillus stearothermophilus and escherichia coli ribosomes. 19724567807
specific recognition of non-initiator regions in rna bacteriophage messengers by ribosomes of bacillus stearothermophilus. 19734570382
mode of action of n-tosyl-l-phenylalanylchloromethane on the elongation protein-synthesizing s 3 factor from bacillus stearothermophilus. 19734570973
survival of microorganisms in laundered polyester-cotton sheeting.the effects of wash-water temperature, cold-water or regular detergent, wash-cycle design, drying, and drying temperature on survival of four microorganisms on polyester-cotton sheeting were examined. escherichia coli t3 bacteriophage survived washing at 24, 35, 46, and 57 c, but not at 68 c. serratia marcescens survived only the lowest three wash temperatures. levels of residual staphylococcus aureus were diminished at the highest two wash temperatures, but survival was substantial even at 68 c ...19734572894
inhibition of spore outgrowth and vegetative growth of bacillus stearothermophilus by dipicolinate.spores of bacillus stearothermophilus germinated in the presence of sodium dipicolinate (dpa) but did not elongate after the emergence stage of outgrowth. vegetative growth also was inhibited by na(2)dpa.19734574703
[demonstration of "free spores" of bacillus stearothermophilus through synchronous sporulation with subsequent endotrophic lysis of sporangia]. 19734581213
discriminatory ribosome rebinding of isolated regions of protein synthesis initiation from the ribonucleic acid of bacteriophage r17.to determine whether bacterial ribosomes recognize a distinguishing feature in the immediate vicinity of actual initiator codons or are directed to these sites through involvement of other portion(s) of the mrna molecule, the interaction between ribosomes and defined (32)p-labeled initiator fragments from r17 rna was studied. when incubated with mixtures of the three sites, ribosomes from bacillus stearothermophilus (which initiate only the a protein on intact phage rna) are able to select out t ...19734582190
the amino acid sequence of the n-terminal region of some 30s ribosomal proteins from escherichia coli and bacillus stearothermophilus: homologies in ribosomal proteins. 19734583836
a study of the thermal stability of ribosomes and biologically active subribosomal particles.1. the ability of escherichia coli ribosomes to function in poly(u)-directed protein synthesis was measured at elevated temperatures by using thermostable supernatant factors from bacillus stearothermophilus. the amount of polyphenylalanine synthesized at 55 degrees c was about the same as at 37 degrees c, but the rate of synthesis was increased approximately fivefold. at 60 degrees c the activity of the ribosomes was halved. 2. e. coli ribosomes can sustain the loss of approx. 10% of the double ...19734584137
study of conditions for the inhibitory effect of n-tosyl-l-phenylalanylchloromethane on protein synthesis and the possibility of existence of different forms of the elongation factor s3 in bacillus stearothermophilus. 19734587077
correlation between 30s ribosomal proteins of bacillus stearothermophilus and escherichia coli. 19734589348
specific modification of arginyl-transfer ribonucleic-acid synthetase from bacillus stearothermophilus as a result of substrate binding. 19734589541
germination of spores of bacillus stearothermophilus induced by analogues of dipicolinate di-anion.the structural specificity required for induction of germination of spores of bacillus stearothermophilus by analogues of dipicolinate in buffer at ph 5.5 is similar to that found previously with spores of bacillus megaterium and calcium chelate salts at ph 8. 4h-pyran-2,6-dicarboxylate, but no other analogue tested, is as effective as dipicolinate di-anion.19744591954
amino-terminal sequences of some escherichia coli 30s ribosomal proteins and functionally corresponding bacillus stearothermophilus ribosomal proteins.amino-terminal sequences of five purified escherichia coli 30s ribosomal proteins (s4, s9, s10, s16, and s20) were compared with those of their functionally corresponding bacillus stearothermophilus ribosomal proteins identified previously by the reconstitution technique. an automatic edman degradation method was used for sequence determinations. the sequence of the first 30 residues is presented, except that only the first 25 residues are shown for the s20 pair. substantial (40 to 70%) sequence ...19744595196
cistron specificity of 30s ribosomes heterologously reconstituted with components from escherichia coli and bacillus stearothermophilus. 19744597073
identification of chloramphenicol-binding protein in escherichia coli ribosomes by affinity labeling.monoiodoamphenicol, a synthetic analogue of chloramphenicol, has been shown by competition experiments with chloramphenicol and lincomycin to bind at the same site of 70s ribosomes as chloramphenicol. at - 2 degrees it forms a 1:1 complex with 70s ribosomes having a value of k (7.5 x 10(4) m(-1)) that is one order of magnitude lower than that of chloramphenicol. at 37 degrees , monoiodoamphenicol irreversibly inhibits the protein-synthesizing activity of e. coli ribosomes. it is shown that the a ...19734599619
[heat activation of bacillus stearothermophilus spores endotrophically treated with bivalent cations]. 19744600359
incorrect aminoacylations involving trnas or valyl-trna synthetase from bacillus stearothermophilus. 19744604598
proceedings: regulation of gluconeogenesis in bacillus stearothermophilus. 19734604960
a stringent factor activity associated with the 50 s ribosomal subunit of bacillus stearothermophilus. 19744606153
procaryotic ribosomal proteins: n-terminal sequence homologies and structural correspondence of 30 s ribosomal proteins from escherichia coli and bacillus stearothermophilus. 19744607606
specific ribonucleoprotein fragments from the 30-s ribosomal subunits of halobacterium cutirubrum, escherichia coli and bacillus stearothermophilus. 19724609470
a comparison of purified valyl-transfer ribonucleic acid synthetase from bacillus stearothermophilus and from escherichia coli.the purification of valyl-trna synthetase from bacillus stearothermophilus is described. the protein was greater than 90% homogeneous on polyacrylamide-gel electrophoresis after more than 850-fold purification. it has a molecular weight of 110000, and no evidence was found for the presence of subunit structure. the properties of the purified enzyme were compared with those of purified valyl-trna synthetase from escherichia coli. the thermal stability, ph-stability and dependence of activity on t ...19744614793
the fine-structural properties of the amino-terminal region of the 50s ribosomal "a" protein from bacillus stearothermophilus. 19744619940
substrate inactivation of fructose-i,6-diphosphate aldolase from bacillus stearothermophilus. 19724622933
alumina--atached spores of bacillus stearothermophilus for the control of sterilization process. 19724627038
alumina--attached spores of bacillus stearothermophilus for the control of sterilization process. 19724630308
association of ribosomal subunits. 3. properties of the 30 s-50 s couples produced in vitro by the association factor from bacillus stearothermophilus. 19734633533
isolation of the intact strands of the deoxyribonucleic acid of tphi3, a bacteriophage for bacillus stearothermophilus. 19724639277
subunit structure of the thermophilic aminopeptidase i from bacillus stearothermophilus. 19724655250
the effect of spermine on the thermal denaturation profiles of ribosomal rna and of ribosomes from bacillus stearothermophilus in the presence of physiological concentrations of cations. 19724658856
inhibition of peptidoglycan synthesis by the antibiotic diumycin a.diumycin a, a new antibiotic, was found to inhibit cell wall synthesis by staphylococcus aureus, a phenomenon accompanied by accumulation of uridine-5'-diphosphate-n-acetyl-muramyl-pentapeptide. the antibiotic inhibited in vitro peptidoglycan synthesis by particulate preparations of bacillus stearothermophilus and escherichia coli by preventing the utilization of n-acetyl-glucosamine-n-acetyl-muramyl-pentapeptide. in contrast to vancomycin, the antibiotics diumycin, prasinomycin, moenomycin, 11. ...19724670441
use of bacillus stearothermophilus as a biological indicator. 19734686286
differential heat stabilities of bacillus amylase.seventeen cultures representing eight strains of bacillus coagulans and twelve cultures representing eight strains of bacillus stearothermophilus did not yield a culture that would produce amylase at both 37 and 55 c.19734688668
the effect of growth temperatures on the in vivo ribose methylation of bacillus stearothermophilus transfer rna. 19734689778
restriction and modification of bacteriophage in bacillus stearothermophilus.host-controlled restriction and modification of tp-1c phage and infectious phage dna occurs in bacillus stearothermophilus and is subject to control by tp-8 or tp-12 prophage.19734700514
test objects containing bacillus stearothermophilus spores for the monitoring of antimicrobial treatment in steam autoclaves. results obtained with commercial preparations and with the authors' own test objects. 19734702494
d-alanine carboxypeptidases of bacillus stearothermophilus: solubilisation of particulate enzymes and mechanism of action of penicillin. 19734710761
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