Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| studies of aeromonas formicans crawford comb. nov. from soluble oil emulsions. | 1957 | 13416178 | |
| taxonomic relationships among the pseudomonads. | colwell, r. r. (university of washington, seattle), and j. liston. taxonomic relationships among the pseudomonads. j. bacteriol. 82:1-14. 1961.-an electronic computer technique, utilizing the adansonian principle that every feature should have equal weight, was applied in an effort to derive a taxonomy of the pseudomonas-achromobacter group of gram-negative, asporogenous, rodlike bacteria. the validity of the general method was tested by an analysis of 40 well defined strains, principally derive ... | 1961 | 13694873 |
| taxonomic analysis with the electronic computer of some xanthomonas and pseudomonas species. | colwell, r. r. (university of washington, seattle) and j. liston. taxonomic analysis with the electronic computer of some xanthomonas and pseudomonas species. j. bacteriol. 82:913-919. 1961.-forty organisms, including 16 xanthomonas spp., 5 strains of pseudomonas solanacearum, 4 strains of p. aeruginosa, 6 strains of p. fluorescens, 5 freshly isolated strains of pseudomonads, as well as the american type culture collection strains of flavobacterium aquatile, vibrio cuneatus, vibrio metschnikovii ... | 1961 | 13880721 |
| [the action in vitro of kanamycin on aeromonas punctata]. | 1961 | 13880924 | |
| [the production of putrefaction of the caudal fin in fish by the action of aeromonas punctata]. | 1961 | 13880926 | |
| fermentation reactions of pseudomonas caviae and its serological relationship to aeromonads. | liu, pinghui v. (university of louisville school of medicine, louisville, ky.). fermentation reactions of pseudomonas caviae and its serological relationship to aeromonads. j. bacteriol. 83:750-753. 1962.-pseudomonas caviae was found to ferment carbohydrates instead of oxidizing them. this characteristic would seem to eliminate this organism from the genus pseudomonas and place it in the genus aeromonas. it was identified as a. liquefaciens, by its species-specific extracellular antigens and by ... | 1962 | 14465955 |
| the c-terminal module of chi1 from aeromonas caviae cb101 has a function in substrate binding and hydrolysis. | the chitinase gene chi1 of aeromonas caviae cb101 encodes an 865-amino-acid protein (with signal peptide) composed of four domains named from the n-terminal as an all-beta-sheet domain chin, a triosephosphate isomerase (tim) catalytic domain, a function-unknown a region, and a putative chitin-binding domain (chbd) composed of two repeated sequences. the n-terminal 563-amino-acid segment of chi1 (chi1deltaadeltachbd) shares 74% identity with chia of serratia marcescens. by the homology modeling m ... | 2003 | 14635132 |
| cloning, expression, and cell surface localization of paenibacillus sp. strain w-61 xylanase 5, a multidomain xylanase. | we have shown that a xylan-degrading bacterium, w-61, excretes multiple xylanases, including xylanase 5 with a molecular mass of 140 kda. here, we emend the previously used classification of the bacterium (i.e., aeromonas caviae w-61) to paenibacillus sp. strain w-61 on the basis of the nucleotide sequence of the 16s rrna gene, and we clone and express the xyn5 gene encoding xylanase 5 (xyn5) in escherichia coli and study the subcellular localization of xyn5. xyn5 encodes 1,326 amino acid residu ... | 2003 | 14660338 |
| enteropathogenic activity and invasion of hep-2 cells by aeromonas caviae clinical isolates. | twenty aeromons caviae isolates from stool of children with diarrhea symptoms were examined for virulence-associated properties: production of cytotoxic and cytotonic toxins, and invasive ability. most of a. caviae strains were cytotoxic to vero and cho cells and produced cytotonic toxins which caused elongation of cho cells. moreover, five of a. caviae strains revealed invasive ability towards hep-2 cells. | 2003 | 14743980 |
| [virulence factors of clinical aeromonas caviae isolates]. | aeromonas caviae, an ubiquitous aquatic organism, has long been considered to be of low pathogenicity, and its virulence mechanisms are still not clearly understood. twenty-eight a. caviae isolates of clinical origin, most often monomicrobic, were identified in our university hospital over a four year period. patients, mostly immunocompromised, were: eight diarrhoeal infants, 13 diarrhoeal adults, seven bacteraemic adults. adults were frequently suffering from underlying intestinal malignancy, h ... | 2004 | 14761709 |
| coexpression of genetically engineered 3-ketoacyl-acp synthase iii (fabh) and polyhydroxyalkanoate synthase (phac) genes leads to short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production from glucose in escherichia coli jm109. | polyhydroxyalkanoates (phas) can be divided into three main types based on the sizes of the monomers incorporated into the polymer. short-chain-length (scl) phas consist of monomer units of c3 to c5, medium-chain-length (mcl) phas consist of monomer units of c6 to c14, and scl-mcl phas consist of monomers ranging in size from c4 to c14. although previous studies using recombinant escherichia coli have shown that either scl or mcl pha polymers could be produced from glucose, this study presents t ... | 2004 | 14766582 |
| type iii secretion system genes in clinical aeromonas isolates. | we have identified the genes ascf and ascg, which encode components of a putative type iii secretion system (ttss) in aeromonas: we investigated the distribution of these and other ttss genes in 84 clinical isolates and found hybridizing sequences in 50% of the strains, with a higher prevalence in aeromonas hydrophila and aeromonas veronii than in aeromonas caviae. | 2004 | 15004096 |
| aeromonas caviae keratitis associated with contact lens wear. | we report the first case of bilateral contact lens-related aeromonas caviae keratitis associated with a. caviae contamination of the contact lens case. the presence of virulence factors produced by aeromonas species was also investigated. | 2004 | 15019388 |
| aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces. | aeromonas spp. (gram-negative, aquatic bacteria which include enteropathogenic strains) have two distinct flagellar systems, namely a polar flagellum for swimming in liquid and multiple lateral flagella for swarming over surfaces. only approximately 60% of mesophilic strains can produce lateral flagella. to evaluate flagellar contributions to aeromonas intestinal colonization, we compared polar and lateral flagellar mutant strains of a diarrheal isolate of aeromonas caviae for the ability to adh ... | 2004 | 15039313 |
| a secreted metallo protease from aeromonas hydrophila exhibits prothrombin activator activity. | detection, purification, and partial characterization of a protease from aeromonas hydrophila capable of cleaving prothrombin into active thrombin is described. the protease has been characterized with respect to enzymatic characteristics such as optimum reaction conditions for prothrombin activation, usage of additional substrates, as well as sensitivity against inhibitors. the protease activity can reversibly be inhibited by me2+ chelating agents like ethylenediamine tetraacetic acid. the enzy ... | 2004 | 15091004 |
| prevalence, serotype distribution, antibiotic susceptibility and genetic profiles of mesophilic aeromonas species isolated from hospitalized diarrhoeal cases in kolkata, india. | a comprehensive study was performed to examine incidence, species distribution, drugs sensitivity, virulence genes and molecular fingerprints of aeromonas species isolated from patients with acute diarrhoea over a period of 2 years in kolkata, india. following the aerokey ii scheme, more than 95 % of strains were identified to species level. seven different species were encountered in this study, with aeromonas caviae being dominant, followed by aeromonas hydrophila and aeromonas veronii biovar ... | 2004 | 15150333 |
| purification and characterization of a novel metalloprotease isolated from aeromonas caviae. | a novel protease produced by aeromonas caviae was purified and characterized. the molecular weight of the protease (ap19) was estimated as 19 kda on sds-polyacrylamide gel electrophoresis. the protease activity was not inhibited completely by heating at 100 degrees c for 15 min. the proteolytic activities were inhibited by metalloprotease inhibitor. the n-terminal amino acid sequence of ap19 was vtasvsfsgrctn. ap19 did not activate aeromonas proaerolysin, and did not show fluid accumulation in t ... | 2004 | 15268947 |
| [discordancies between classical and api 20e microtest biochemical identification of vibrio and aeromonas strains]. | the present study reveals some discordancies of diagnosis considering vibrionaceae strains when tested by comparison with api 20e (biomerieux) system and classical biochemical reactions, aspects already mentioned in the literature by other authors. if any misidentification has not an important impact (e.g. identification of aeromonas schubertii as a. sobria), others as misidentification of aeromonas as vibrio strains or the failure to recognize vibrio strains raise major epidemiological problems ... | 2003 | 15341329 |
| increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of saccharomyces cerevisiae through modification of the beta-oxidation cycle. | short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from aeromonas caviae and ralstonia eutropha in the peroxisomes. the quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward r-3-hydroxybutyryl coenzyme a. | 2004 | 15345460 |
| detection, integration and persistence of aeromonads in water distribution pipe biofilms. | the occurrence of aeromonas spp. within biofilms formed on stainless steel (ss), unplasticized polyvinyl chloride (upvc) and glass (gl) substrata was investigated in modified robbins devices (mrd) in potable (mrd-p) and recycled (mrd-r) water systems, a biofilm reactor (br) and a laboratory-scale pipe loop (pl) receiving simulated recycled wastewater. no aeromonads were isolated from the mrd-p whereas 3-10% of ss and upvc coupons (mean 3.85 cfu cm(-2) and 12.8 cfu cm(-2), respectively) were aero ... | 2004 | 15387132 |
| engineered aeromonas hydrophila for enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with alterable monomers composition. | aeromonas hydrophila 4ak4 produces poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) containing 3-hydroxybutyrate (3hb) and about 15 mol% 3-hydroxyhexanoate (3hhx) from dodecanoate. to study the factors affecting the monomer composition and phbhhx content, genes encoding phasin (phap), pha synthase (phac) and (r)-specific enoyl-coa hydratase (phaj) from aeromonas punctata (formerly named aeromonas caviae) were introduced individually or jointly into a. hydrophila 4ak4. the phac gene increas ... | 2004 | 15451119 |
| an unusually high level of quinolone resistance associated with type ii topoisomerase mutations in quinolone resistance-determining regions of aeromonas caviae isolated from diarrhoeal patients. | we examined 158 strains belonging to different aeromonas species isolated from hospitalized acute diarrhoea cases for susceptibility to quinolones. compared to other species, a high percentage of the a. caviae strains were resistant to ciprofloxacin and norfloxacin. based on mic values, 6 a. caviae strains were selected and the nucleotide sequences for the quinolone-resistant-determining regions (qrdrs) of gyra, gyrb and parc genes were analysed. in resistant strains, double mutations (ser(83)-- ... | 2004 | 15567276 |
| complete nucleotide sequence of the conjugative tetracycline resistance plasmid pfbaot6, a member of a group of incu plasmids with global ubiquity. | this study presents the first complete sequence of an incu plasmid, pfbaot6. this plasmid was originally isolated from a strain of aeromonas caviae from hospital effluent (westmorland general hospital, kendal, united kingdom) in september 1997 (g. rhodes, g. huys, j. swings, p. mcgann, m. hiney, p. smith, and r. w. pickup, appl. environ. microbiol. 66:3883-3890, 2000) and belongs to a group of related plasmids with global ubiquity. pfbaot6 is 84,748 bp long and has 94 predicted coding sequences, ... | 2004 | 15574953 |
| antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish. | motile aeromonads isolated from the intestines of farm-raised freshwater fish such as catla catla, labeo rohita and ctenopharyngodon idella have been characterized to species level. morphological and physiological grouping revealed 61% aeromonas hydrophila, 30% aeromonas caviae, 7% aeromonas sobria and 2% which remained unidentified. hemolytic activity was detected mostly in a. hydrophila, while only half of the a. sobria and a. caviae showed this activity. antibiotic resistance patterns of the ... | 2005 | 15747443 |
| [aeromonas caviae infection mimicking inflammatory bowel disease in a child]. | a 10-year-old girl presented with a pancolitis with watery bloody diarrhoea. her family history revealed the presence of inflammatory bowel disease. whilst awaiting the diagnostic tests results, the stool cultures showed aeromonas caviae. the disease resolved spontaneously. after 24 months there were no further signs of illness, notably no inflammatory bowel disease. this case represents a more severe form than the mild diarrhoea that is usually described in the literature. in a retrospective se ... | 2005 | 15819138 |
| adaptational changes in cellular fatty acid branching and unsaturation of aeromonas species as a response to growth temperature and salinity. | the effects of growth temperature and salinity on the cellular fatty acids were investigated on aeromonas caviae, aeromonas hydrophila and aeromonas sobria. under optimal growth conditions, fatty acids patterns were dominated by even-numbered chains c(16:0), c(16:1cis9), c(18:1cis11), c(12:0) and c(14:0). growth temperature modifications induced, in the three aeromonas species, important changes in fatty acid (i) unsaturation, (ii) branching and (iii) chain length. an important decrease in the c ... | 2005 | 15925007 |
| enhancement of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production in the transgenic arabidopsis thaliana by the in vitro evolved highly active mutants of polyhydroxyalkanoate (pha) synthase from aeromonas caviae. | in this study, the enhancement of photosynthetic pha production was achieved using the highly active mutants of pha synthase created by the in vitro evolutionally techniques. the wild-type and mutated pha synthase genes from aeromonas caviae were introduced into arabidopsis thaliana together with the nadph-dependent acetoacetyl-coa reductase gene from ralstonia eutropha. expression of the highly active mutated pha synthase genes, n149s and d171g, led to an 8-10-fold increase in pha content in th ... | 2005 | 16004454 |
| [aeromonas caviae infection mimicking inflammatory bowel disease in a child]. | 2005 | 16008044 | |
| [aeromonas caviae infection mimicking inflammatory bowel disease in a child]. | 2005 | 16008045 | |
| biosynthesis and characterization of poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) from palm oil products in a wautersia eutropha mutant. | palm kernel oil, palm olein, crude palm oil and palm acid oil were used for the synthesis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] by a mutant strain of wautersia eutropha (formerly ralstonia eutropha) harboring the aeromonas caviae polyhydroxyalkanoate (pha) synthase gene. palm kernel oil was an excellent carbon source for the production of cell biomass and p(3hb-co-3hhx). about 87% (w/w) of the cell dry weight as p(3hb-co-3hhx) was obtained using 5 g palm kernel oil/l ... | 2005 | 16215858 |
| the processing of high-molecular-weight xylanase (xyne, 110 kda) from aeromonas caviae me-1 to 60-kda xylanase (xyne60) in escherichia coli and purification and characterization of xyne60. | a xylanase gene (xyne) encoding xyne (110 kda) was cloned from a lambda phage genomic library of aeromonas caviae me-1 which is a multiple-xylanase-producing bacterium. upon nucleotide sequence analysis, we found that xyne comprises 2823 by and encodes a protein of 941 amino acid residues (104,153 da), which was similar to endo-beta-1,4-xylanases which are categorized to glycosyl hydrolase family 10. an escherichia coli transformant that harbored pxed30 carrying xyne produced 110-, 84-, 72-, and ... | 2003 | 16233373 |
| a cytoplasmic xylanase (xynx) of aeromonas caviae me-1 is released from the cytoplasm to the periplasm by osmotic downshock. | aeromonas caviae me-1 is a multiple xylanase-producing gram-negative bacterium which was isolated from the gut contents of a wild silkworm, samia cynthia pryeri. one of the xylanases produced by a. caviae me-1, xynx (38 kda, family 10 xylanase), hydrolyzes xylan to xylobiose and xylotetraose as final degradation products. generally, xylanases are extracellular or cell surface enzymes. however, xynx is not exported to the extracellular fluid by a. caviae me-1 and an escherichia coli transformant ... | 2003 | 16233445 |
| processing of xyne (110-kda) of aeromonas caviae me-1 to 72-kda xylanase in escherichia coli transformant. | an extracellular 72-kda xylanase from an escherichia coli transformant that harbored pxed30 carrying xyne of aeromonas caviae me-1 was purified by extraction following sds-page to electrophoretic homogeneity. the analysis of n-terminal 10 amino acid residues (gly-ala-arg-ala-gln-ala-ala-ala-asp-val) revealed a 72-kda product derived by the cleavage of gly26-gly27 at the n-terminal region of 110 kda xyne. the 72-kda xylanase from a. caviae me-1 was found to have the same sequence as that of the n ... | 2003 | 16233547 |
| putative exposed aromatic and hydroxyl residues on the surface of the n-terminal domains of chi1 from aeromonas caviae cb101 are essential for chitin binding and hydrolysis. | chitinase chi1 of aeromonas caviae cb101 possesses chitin binding sites at both its n and c termini. four putative exposed residues aligned in a line on the surface of the n-terminal domains of chi1 were found to contribute to the enzyme-chitin binding and hydrolysis via site-directed mutagenesis. also, it was found that chi1 requires the cooperation of the n- and c-terminal domains to bind fully with crystalline and colloidal chitin. | 2005 | 16269803 |
| [synthesis of copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate by aeromonas hydrophila wq and its molecular basis]. | aeromonas hydrophila wq isolated from lake water was found to be able to synthesize polyhydroxyalkanoates (pha) copolymer consisting of 3-hydroxybutyrate (hb) and 3-hydroxyhexanoate (hhx) (phbhhx). lauric acid was found to be the most suitable carbon source for cell growth and phbhhx accumulation. the bacteria accumulated 49% phbhhx containing 6% hhx in terms of cell dry weight when grown on lauric acid for 72 h. 42% phbhhx consisting of 14% hhx was obtained with 5 g/l glucose and 10 g/l lauric ... | 2003 | 16276907 |
| identification of unusual bacterial glycosylation by tandem mass spectrometry analyses of intact proteins. | the characterization of protein glycosylation can be a complex and time-consuming procedure, especially for prokaryote o-linked glycoproteins, which often comprise unusual oligosaccharide structures with no known glycosylation motif. in this report, we describe a "top-down" approach that provides information on the extent of glycosylation, the molecular masses, and the structure of oligosaccharide residues on bacterial flagella, important structural proteins involved in the motility of pathogeni ... | 2005 | 16316188 |
| purification and properties of beta-1, 4-xylanase from aeromonas caviae w-61. | aeromonas caviae w-61, which was isolated from water samples at the faculty of agriculture, tohoku university, produced beta-1, 4-xylanase (1,4-beta-d-xylan xylanohydrolase; ec 3.2.1.8) extracellularly. the xylanase was purified to homogeneity by using deae-sephadex a-50, cm-sephadex c-50, and sephadex g-100 column chromatographies. the molecular weight of the purified enzyme was estimated to be 22,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the isoelectric point of the enz ... | 1991 | 16348410 |
| purification and characterization of aeromonas caviae me-1 xylanase v, which produces exclusively xylobiose from xylan. | a xylanase, which produces exclusively xylobiose from oat spelt and birch xylans, was isolated from the culture medium of aeromonas caviae me-1. the enzyme (xylanase v) was purified by ammonium sulfate fractionation, hydrophobic interaction, and ion-exchange and gel filtration chromatographies. the homogeneity of the final preparation was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and agarose gel electrofocusing. the molecular mass and isoelectric point of the xyla ... | 1994 | 16349182 |
| identification of aeromonas species isolated from freshwater fish with the microplate hybridization method. | aeromonas isolates were obtained from the intestinal tracts of six species of cultured freshwater fish and identified on the basis of their genotypic and phenotypic characters. the microplate hybridization method could differentiate type strains of aeromonas species and related bacteria. dna-dna hybridization analysis showed that 65 aeromonad isolates were 72 to 100% related with either aeromonas caviae, aeromonas hydrophila, aeromonas jandaei, aeromonas sobria, or aeromonas veronii. as many as ... | 1994 | 16349363 |
| pulsed-field gel electrophoresis profiles of aeromonads isolated from healthy and diseased helix aspersa from french snail farms. | the xbai digestion patterns of chromosomal dna of 42 aeromonads isolated from french breeding snails during a new epizootic disease, which rapidly progressed to death during the summer of 1994, were analyzed by pulsed-field gel electrophoresis. biochemical identification to species level was also performed. interestingly, we found that 76% of the aeromonads isolated from diseased snails clustered into a unique pulsotype (p1) whatever their geographic origin, and were assessed to belong to aeromo ... | 2005 | 16391663 |
| [beta-lactamases of gram negative bacteria: never-ending clockwork!]. | the acquired resistance against the wide-spectrum and highly stable beta-lactams including third-generation cephalosporins (3gc) and carbapenems is constinuously increasing and widespead with the discovery of various plasmid-encoded, or genes cassette or integrons coding for a novel beta-lactamase, always a major mechanism of resistance. to explain resistance against 3gc, with the continuing story with tem and shv mutated enzymes, several types of esbl (class a) emerge the ctx-m type, at least c ... | 2006 | 16420989 |
| crystallization and preliminary x-ray crystallographic studies of xynx, a family 10 xylanase from aeromonas punctata me-1. | xylanases catalyze the hydrolysis of beta-1,4-glycosidic linkages within the xylan backbone. xynx is a xylanase from aeromonas punctata me-1 and belongs to glycoside hydrolase family 10. while most xylanases show endo-type catalytic activities, xynx shows exo-like catalytic activities, selectively producing xylobiose from birchwood xylan. in this study, xynx was crystallized by the hanging-drop vapour-diffusion method. the crystals belonged to the orthorhombic space group p2(1)2(1)2(1), with uni ... | 2005 | 16511010 |
| xylanase iv, an exoxylanase of aeromonas caviae me-1 which produces xylotetraose as the only low-molecular-weight oligosaccharide from xylan. | a novel xylanase (xylanase iv) which produces xylotetraose as the only low-molecular-weight oligosaccharide from oat spelt xylan was isolated from the culture medium of aeromonas caviae me-1. by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the xylanase iv molecular weight was 41,000. xylanase iv catalyzed the hydrolysis of oat spelt xylan, producing exclusively xylotetraose. the acid hydrolysate of the product gave d-xylose. the enzyme did not hydrolyze either p-nitrophenyl-(beta)- ... | 1995 | 16535010 |
| distribution of aeromonas species in the intestinal tracts of river fish. | aeromonas isolates were obtained from fish intestines, water, and sediments from an urban river and identified by the dna-dna microplate hybridization method. the isolates were aeromonas veronii (22%), aeromonas caviae (18%), aeromonas hydrophila (13%), aeromonas sobria (8%), aeromonas jandaei (7%), and other aeromonas spp. (33%). aeromonas species occurred at high densities with high incidences, regardless of season. the results strongly suggest that aeromonads are indigenous in fish intestines ... | 1995 | 16535171 |
| [aeromonas associated with an acute diarrhea outbreak in são bento do una, pernambuco]. | an acute diarrhea outbreak, with 2170 cases, was described during january to july, 2004, in são bento do una, pernambuco. 582 stools were examined and an enteric pathogen was recovered in 25% (145 patients). aeromonas species were the most frequent (114-19.5%) and the main isolates were aeromonas caviae (57-9.8%), aeromonas veronii biovar sobria (23-3.9%), aeromonas veronii biovar veronii (15-2.6%) and other species (19-3.2%). the other isolated enteropathogens were vibrio cholerae o1-ogawa toxi ... | 2006 | 16699653 |
| rabbit meat as a source of bacterial foodborne pathogens. | even though worldwide production of rabbit meat is >1,000,000 tons, little information is available for rabbit meat microbiology. this study provides data on the prevalence of salmonella, escherichia coli o157:h7, yersinia enterocolitica, listeria spp., motile aeromonas spp., and staphylococcus aureus on rabbit meat. a total of 24 rabbit carcasses from two abattoirs and 27 rabbit meat packages from supermarket displays were examined. in addition to culturing methods, associated virulence genes w ... | 2006 | 16715811 |
| clinical significance and distribution of putative virulence markers of 116 consecutive clinical aeromonas isolates in southern taiwan. | the objectives of this study were to elucidate the clinical manifestations of aeromonas infections and the association of putative virulence genes with clinical invasiveness. | 2007 | 16716402 |
| biochemical and molecular characterization of tetracycline-resistant aeromonas veronii isolates from catfish. | eighty-one tetracycline-resistant aeromonas sp. strains were isolated from farm-raised catfish. morphological and biochemical characteristics indicated that 23 of the 81 aeromonads were aeromonas hydrophila, 7 isolates were aeromonas trota, 6 isolates were aeromonas caviae, 42 isolates were aeromonas veronii, and 3 isolates were aeromonas jandaei. however, the alui and mboi restriction fragment length polymorphism (rflp) patterns of the pcr-amplified 1.4-kb 16s rrna gene from all 81 tetracycline ... | 2006 | 17021193 |
| virulence factors of a. caviae strains isolated from acute diarrheic disease in cuba. | fifty aeromonas caviae strains from intestinal infection in different cuban provinces were identified by the aerokey ii method and virulence factors were investigated. the strains did not produce haemolysins but other exoenzymes such as proteases, lipases, and dnases; additionally, all isolates adhered to the hep-2 cell line by the carrello method and this did not correlate with other virulence factors presence which demonstrates that the haemolysin phenotypic expression is not necessary for the ... | 2002 | 17061509 |
| occurrence and diversity of integrons and beta-lactamase genes among ampicillin-resistant isolates from estuarine waters. | the aim of the present study was to assess the occurrence and molecular diversity of beta-lactamase genes and integrons among gram-negative ampicillin-resistant bacteria from ria de aveiro. ampicillin-resistant isolates were selected and subjected to genotyping using rep-pcr. representatives from each rep pattern were affiliated with the following taxa by sequencing the 16s rrna gene: aeromonas caviae, a. hydrophila, a. media, a. molluscorum, a. veronii, a. salmonicida, aeromonas sp., pseudomona ... | 2006 | 17125975 |
| assessment of toxicity of a glyphosate-based formulation using bacterial systems in lake water. | a new aeromonas bioassay is described to assess the potential harmful effects of the glyphosate-based herbicide, roundup, in the albufera lake, a protected area near valencia. viability markers as membrane integrity, culturability and beta-galactosidase production of aeromonas caviae were studied to determine the influence of the herbicide in the bacterial cells. data from the multifactor analysis of variance test showed no significant differences (p>0.05) between a. caviae counts of viability m ... | 2007 | 17270238 |
| poly[(r)-3-hydroxybutyrate] formation in escherichia coli from glucose through an enoyl-coa hydratase-mediated pathway. | in this study, a new metabolic pathway for the synthesis of poly[(r)-3-hydroxybutyrate] [p(3hb)] was constructed in a recombinant escherichia coli strain that utilized forward and reverse reactions catalyzed by two substrate-specific enoyl-coa hydratases, r-hydratase (phaj) and s-hydratase (fadb), to epimerize (s)-3hb-coa to (r)-3hb-coa via a crotonyl-coa intermediate. the r-hydratase gene (phaj(ac)) from aeromonas caviae was coexpressed with the pha synthase gene (phac(re)) and 3-ketothiolase g ... | 2007 | 17298899 |
| characterization of cytotoxin-producing aeromonas caviae (strain ht10) isolated from a sulfur spring in orissa, india. | during a study of microbial diversity, a bacterial strain designated ht10, was isolated from sediment collected from an unexplored sulfur spring at athamallik, orissa, india. various biochemical tests and 16s rdna sequence analysis revealed that strain ht10 is aeromonas caviae. the growth temperature of this strain ranged from 12 to 43 degrees c and the optimum temperature was 30 degrees c. the strain ht10 showed cytotoxic and alpha-hemolytic activity. this is the first report on the isolation o ... | 2007 | 17309514 |
| screening of genes involved in chitinase production in aeromonas caviae cb101 via transposon mutagenesis. | to find genes involved in chitinase production in chitinolytic bacterium aeromonas caviae cb101. | 2007 | 17309612 |
| non-structural flagella genes affecting both polar and lateral flagella-mediated motility in aeromonas hydrophila. | an aeromonas hydrophila ah-3 minitn5 mutant unable to produce polar and lateral flagella was isolated, in which the transposon was inserted into a gene whose encoded protein was an orthologue of the campylobacter jejuni motility accessory factor (maf) protein. in addition to this gene, several other related genes were found in this cluster that was adjacent to the region 2 genes of the polar flagellum. mutation of the a. hydrophila ah-3 maf-2, neub-like, flmd or neua-like genes resulted in non-m ... | 2007 | 17379726 |
| importance of flagella and enterotoxins for aeromonas virulence in a mouse model. | a genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaa/flab, flag), lateral flagella (lafa), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. when 16 aeromonas hydrophila strains, seven aeromonas veronii strains, and seven aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal inj ... | 2007 | 17496975 |
| cystitis caused by aeromonas caviae. | aeromonas sp. organisms rarely cause urinary tract infection. we report for the first time a case of urinary tract infection caused by a. caviae in an adult patient with a history of increased frequency of urination, dysuria, hematuria, and weight loss. | 2007 | 17522269 |
| variation in copolymer composition and molecular weight of polyhydroxyalkanoate generated by saturation mutagenesis of aeromonas caviae pha synthase. | amino acid substitutions at two residues downstream from the active-site histidine of polyhydroxyalkanoate (pha) synthases are effective for changing the composition and the molecular weight of pha. in this study, saturation mutagenesis at the position ala505 was applied to pha synthase (phacac) from aeromonas caviae to investigate the effects on the composition and the molecular weight of pha synthesized in ralstonia eutropha. the copolymer composition and molecular weight of pha were varied by ... | 2007 | 17541931 |
| [isolation and identification of species from the genera aeromonas, vibrio, and plesiomonas from extraintestinal samples in cuba]. | extraintestinal infections caused by the genera aeromonas, vibrio and plesiomonas have high morbidity and mortality rates in different areas of world. from january 2002 to december 2003, the national reference laboratory for acute diarrhoeal diseases of the pedro kourí tropical medicine institute received 95 gram-negative, facultative anaerobic, oxidase positive bacilli strains from different extraintestinal specimen (blood, ear exudates, infected wounds, conjunctive exudates, urine, and cathete ... | 2007 | 17554439 |
| characterization of aeromonas caviae and a. veronii by standardized cellular protein electrophoretic patterns. | aeromonas caviae and a. veronii recovered from clinical and environmental samples were characterized by sds-page of whole cell proteins. all strains were typable and showed unique banding patterns. a high intra-specific diversity within these two microorganisms was revealed. separation of the polish strains from the hong-kong isolates was observed. a. caviae and a. veronii were not distinguishable with respect to their ecological origin. however, strains with very similar protein patterns could ... | 2007 | 17571798 |
| interactions of clinical and environmental aeromonas isolates with caco-2 and ht29 intestinal epithelial cells. | evaluation of adherence and invasion of aeromonas spp. to human colon carcinoma cell lines caco-2 and ht29 and assessment of cytotoxic activity. | 2007 | 17897383 |
| virulence potential and genetic diversity of aeromonas caviae, aeromonas veronii, and aeromonas hydrophila clinical isolates from mexico and spain: a comparative study. | a comparative study of 109 aeromonas clinical isolates belonging to the 3 species most frequently isolated from patients with diarrhea in mexico and spain was performed to investigate the distribution of 3 prominent toxin genes and the gene encoding flagellin of lateral flagella; 4 well-established virulence factors in the genus aeromonas. the aerolysin-hemolysin toxin genes were the most prevalent, being present in 89% of the total isolates. the ast toxin gene was conspicuously absent from the ... | 2007 | 17898843 |
| construction of a chimeric aminopeptidase by a combination of gene shuffling and mutagenesis. | three chimeric genes were constructed by gene shuffling of aminopeptidases from aeromonas caviae and vibrio proteolyticus. although expressed chimeric enzymes formed inclusion bodies in escherichia coli, the introduction of two amino acid mutations into the chimeric genes by site-saturated mutagenesis and a random mutation on error-prone pcr resulted in solubilization of the chimeric enzyme. in addition, active chimeric enzyme showed a different thermostability and thermoactivity to parental enz ... | 2008 | 17934695 |
| first occurrence of an imp metallo-beta-lactamase in aeromonas caviae: imp-19 in an isolate from france. | we describe the first imp metallo-beta-lactamase in aeromonas caviae: imp-19, which differed from imp-2 by a single amino acid change (arg to ala at position 38). bla(imp-19) was found within a class 1 integron located on a 35-kb plasmid. this is also the first description of an imp producer in france. | 2007 | 17938180 |
| [factors of virulence associated with enteropathogenicity in strains of aeromonas spp. isolated from children with diarrhea in mérida, venezuela]. | the feces of 397 patients with acute diarrheal disease (add) and of other 121 patients without diarrea (control group) were studied in the state of mérida, venezuela, from june 1993 to december 1994. the genus aeromonas was identified in patients with add in 11.83% and in 5.78% of the patients from the control group. on studying the virulence factors described for aeromonas (enterotoxin, cytotoxin, hemaglutinins, cellular hydrofibrosity, and hemolytic activity) in the isolated strains, it was de ... | 2005 | 17966577 |
| sensitive and rapid detection of aeromonas caviae in stool samples by loop-mediated isothermal amplification. | in this study, a loop-mediated isothermal amplification assay targeting the 16s-23s intergenic spacer regions (internal transcribed spacer) of aeromonas caviae was developed. eighteen reference strains and 109 clinical samples were analyzed. the results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods. | 2008 | 18024054 |
| combination of n149s and d171g mutations in aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis. | aeromonas caviae polyhydroxyalkanoate synthase (phac(ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)]. in a previous study, two phac(ac) mutants that have a single amino acid substitution of either asparagine 149 by serine (n149s) or aspartate 171 by glycine (d171g) were isolated as higher active enzymes by means of evolutionary engineering. in this study, ... | 2007 | 18031343 |
| [identification of closely related bacteria via phylogenetic methods]. | to differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrb gene sequences. | 2007 | 18067224 |
| structural characterization of the o-chain polysaccharide of aeromonas caviae atcc 15468 lipopolysaccharide. | the o-chain polysaccharide produced by a mild acid degradation of aeromonas caviae atcc 15468 lipopolysaccharide was found to be composed of l-rhamnose, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose and phosphoglycerol. subsequent methylation and ce-esims analyses and 1d/2d nmr ((1)h, (13)c and (31)p) spectroscopy showed that the o-chain polysaccharide is a high-molecular-mass acidic branched polymer of tetrasaccharide repeating units with a phosphoglycerol substituent having th ... | 2008 | 18068695 |
| unexpected occurrence of plasmid-mediated quinolone resistance determinants in environmental aeromonas spp. | we searched for plasmid-mediated quinolone resistance determinants of the qnr type in several water samples collected at diverse locations from the seine river (paris, france). the qnrs2 genes were identified from aeromonas punctata subsp. punctata and a. media. the qnrs2 gene was located on incu-type plasmids in both isolates, which resulted in increased mic values of quinolones and fluoroquinolones, once they were transferred into escherichia coli. the qnrs2 gene identified in a. punctata was ... | 2008 | 18258115 |
| isolation, identification and application of novel bacterial consortium tj-1 for the decolourization of structurally different azo dyes. | a novel bacterial consortium (tj-1), which could decolorize acid orange 7 (ao7) and manyother azo dyes, was developed. in tj-1 three bacterial strains were identified as aeromonas caviae, proteus mirabilis and rhodococcus globerulus by 16s rrna gene sequence analysis. ao7 decolorization was significantly higher with the use of consortium as compared to the use of individual strains, indicating complementary interactions among these strains. ao7 decolorization was observed under microaerophilic c ... | 2008 | 18289845 |
| degradation of linear alkylbenzene sulfonate by a bacterial consortium isolated from the aquatic environment of argentina. | to isolate and identify linear alkylbenzene sulfonate (las)-degrading bacteria from río de la plata and adjacent waters, and to assay their degradation capability as a consortium and as single organisms. | 2008 | 18355233 |
| cloning, expression, and transglycosylation reaction of paenibacillus sp. strain w-61 xylanase 1. | a xylanase gene, xyn1, which encodes paenibacillus sp. strain w-61 xylanase 1 (xyn1), was cloned in escherichia coli. xyn1 encodes 211 amino acid residues, including 28 amino acid residues of a signal peptide. the deduced amino acid sequence of the mature xyn1 showed 95.7%, 84.0%, and 83.7% identity to family 11 xylanases of aeromonas caviae me-1, paenibacillus sp., and bacillus stearothermophilus respectively. the physico-chemical properties of recombinant xyn1 were very similar to those of int ... | 2008 | 18391461 |
| [characterization of aeromonas spp isolates from newborns hospitalized]. | aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ... | 2008 | 18545840 |
| insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. | wastewater treatment plants (wwtps) are a reservoir for bacteria harbouring antibiotic resistance plasmids. to get a comprehensive overview on the plasmid metagenome of wwtp bacteria showing reduced susceptibility to certain antimicrobial drugs an ultrafast sequencing approach applying the 454-technology was carried out. one run on the gs 20 system yielded 346,427 reads with an average read length of 104 bases resulting in a total of 36,071,493 bases sequence data. the obtained plasmid metagenom ... | 2008 | 18586057 |
| the dihydrolipoamide dehydrogenase of aeromonas caviae st exhibits nadh-dependent tellurite reductase activity. | potassium tellurite (k(2)teo(3)) is extremely toxic for most forms of life and only a limited number of organisms are naturally resistant to the toxic effects of this compound. crude extracts prepared from the environmental isolate aeromonas caviae st catalize the in vitro reduction of teo32- in a nadh-dependent reaction. upon fractionation by ionic exchange column chromatography three major polypeptides identified as the e1, e2, and e3 components of the pyruvate dehydrogenase (pdh) complex were ... | 2008 | 18675788 |
| analysis of 16s rrna gene mutations in a subset of aeromonas strains and their impact in species delineation. | characterization of 999 aeromonas strains using a published 16s rdna rflp identification method showed that 8.1% of the strains produced unexpected (hereafter called "atypical") restriction patterns, making their identification uncertain. atypical patterns were due to the presence of nucleotide polymorphisms among the rrn operons of the 16s rrna gene (so-called microheterogeneities). double sequencing signals at certain positions revealed the nucleotide composition was responsible for the microh ... | 2008 | 18843597 |
| multiplex pcr detection of enterotoxin genes in aeromonas spp. from suspect food samples in northern taiwan. | aeromonads possess an array of virulence factors and are causative agents of a number of human infections. among them, genes of one cytotoxic (act) and two cytotonic (alt, ast) enterotoxins are implicated in a human diarrheal disease. a rapid, specific, simultaneous detection of these enterotoxin genes in suspected food poisoning samples is not yet reported. hence, a multiplex pcr assay was designed to amplify the cytotoxic (act), heat-labile cytotonic (alt), and heat-stable cytotonic (ast) ente ... | 2008 | 18939759 |
| aeromonas spp. human isolates induce apoptosis of murine macrophages. | interactions of aeromonas caviae, aeromonas veronii biotype sobria, and aeromonas hydrophila strains isolated from fecal specimens of humans with gastroenteritis on murine macrophages, j774 cells, were investigated. analyses of cellular morphology and dna fragmentation in phagocytes infected with these strains exhibited typical characteristic features of cells undergoing apoptosis. we observed the morphological changes, including condensation of nuclear chromatin, formation of apoptotic bodies a ... | 2009 | 19005723 |
| characterization of aeromonas encheleia strains isolated from aquatic environments in the czech republic. | characterization and identification of aeromonas strains isolated from surface and underground waters using phenotypic and genotyping methods. | 2009 | 19055626 |
| effects of c-terminal amino acids truncation on enzyme properties of aeromonas caviae d1 chitinase. | c-terminal truncation mutagenesis was used to explore the functional and structural significance of the c-terminal region of aeromonas caviae d1 chitinase (acd1chia). comparative studies between the engineered full-length acd1chia and the truncated mutant (acd1chiak606) included initial rate kinetics, fluorescence and circular dichroism (cd) spectrometric properties, and substrate binding and hydrolysis abilities. the overall catalytic efficiency, k(cat)/k(m), of acd1chiak606 with the 4mu-(glcna ... | 2009 | 19089411 |
| interaction of aeromonas spp. human isolates with murine macrophages. | this study aimed to evaluate phagocytic and bactericidal activity of macrophages originated from murine j774 macrophage cell line against 26 strains of aeromonas caviae, aeromonas veronii biotype sobria and aeromonas hydrophila isolated from human fecal specimens. the analysis of phagocytic index (pi) indicated these strains were phagocytosed in low numbers, below 1.1 x l0(-3) for 65% strains of a. veronii biotype sobria and a. hydrophila. the highest pi value (0.019) was noted in murine macroph ... | 2008 | 19123303 |
| expression of aeromonas caviae st pyruvate dehydrogenase complex components mediate tellurite resistance in escherichia coli. | potassium tellurite (k(2)teo(3)) is harmful to most organisms and specific mechanisms explaining its toxicity are not well known to date. we previously reported that the lpda gene product of the tellurite-resistant environmental isolate aeromonas caviae st is involved in the reduction of tellurite to elemental tellurium. in this work, we show that expression of a. caviae st acee, acef, and lpda genes, encoding pyruvate dehydrogenase, dihydrolipoamide transacetylase, and dihydrolipoamide dehydrog ... | 2009 | 19168030 |
| an aeromonas caviae genomic island is required for both o-antigen lipopolysaccharide biosynthesis and flagellin glycosylation. | aeromonas caviae sch3n possesses a small genomic island that is involved in both flagellin glycosylation and lipopolysaccharide (lps) o-antigen biosynthesis. this island appears to have been laterally acquired as it is flanked by insertion element-like sequences and has a much lower g+c content than the average aeromonad g+c content. most of the gene products encoded by the island are orthologues of proteins that have been shown to be involved in pseudaminic acid biosynthesis and flagellin glyco ... | 2009 | 19218387 |
| chimeric enzyme composed of polyhydroxyalkanoate (pha) synthases from ralstonia eutropha and aeromonas caviae enhances production of phas in recombinant escherichia coli. | chimeric enzymes composed of polyhydroxyalkanoate (pha) synthases from ralstonia eutropha (cupriavidus necator) (phac(re)) and aeromonas caviae (phac(ac)) were constructed. phac(re) is known for its potent enzymatic activity among the characterized pha synthases. phacac has broad substrate specificity and synthesizes short-chain-length (scl)/medium-chain-length (mcl) pha. we attempted to create chimeric enzymes inheriting both of the advantageous properties. among eight chimeras, acre12, with 26 ... | 2009 | 19226108 |
| microbiology of the external ear canal in six african elephants (loxodonta africana). | samples collected from both external ear canals of six adult female african elephants (loxodonta africana) were cultured for fungi, yeasts and aerobic and anaerobic bacteria. all the samples produced heavy growths of several aerobic bacteria, but anaerobic bacteria were rare and no fungi or yeasts were isolated. the most common bacterium isolated was staphylococcus epidermidis, which was cultured from 11 of the 12 ears. acinetobacter calcoaceticus lwoffi, alpha-haemolytic streptococcus and coryn ... | 2009 | 19234325 |
| aeromonas caviae septicemia in immunocompetent gastrointestinal carriers. | aeromonas caviae strains have been isolated from blood and stool cultures of three immunocompetent patients, residents of northern india, who presented with community acquired septicemia without any recent history of diarrhea. cell culture infectivity test performed on hep-2 cells have shown substantial degree of invasiveness in the isolated strains. this case unleashes a possibility of asymptomatic gastrointestinal carriage of such strains of a. caviae in a very large population of india, as se ... | 2008 | 19287850 |
| fermentation and growth kinetic study of aeromonas caviae under anaerobic conditions. | although aeromonas caviae is pathogenic to a broad range of invertebrates including human, frequent in aquatic environments, and potentially vital for acidogenesis in anaerobic digestion, virtually no biokinetic information on its anaerobic growth is at hand. therefore, this study focused on evaluating its anaerobic growth kinetics on glucose. to provide a set of relevant biokinetic coefficients for modeling, a combination of curve fitting and numerical modeling was used. microcultivations were ... | 2009 | 19343339 |
| prevalence and transmission of antimicrobial resistance among aeromonas populations from a duckweed aquaculture based hospital sewage water recycling system in bangladesh. | in order to investigate the influence of a duckweed aquaculture based hospital sewage water recycling plant on the prevalence and dissemination of antibiotic resistance, we made use of an existing collection of 1,315 aeromonas isolates that were previously typed by the biochemical fingerprinting php-ae system. in these treatment plant, hospital raw sewage water is first collected in a settlement pond (referred to as sewage water in this study) and is then transferred to a lagoon, where the duckw ... | 2009 | 19459063 |
| aeromonas taiwanensis sp. nov. and aeromonas sanarellii sp. nov., clinical species from taiwan. | two clinical aeromonas strains (a2-50(t) and a2-67(t)) recovered from the wounds of two patients in taiwan could not be assigned to any known species of this genus based on their 16s rrna gene sequences, which showed similarities of 99.6-99.8 % to those of the type strains of aeromonas caviae, a. trota and a. aquariorum. the rpod phylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species being a. caviae, the type strain of which showed 93. ... | 2010 | 19819994 |
| plasmid-mediated qnrs2 determinant in an aeromonas caviae isolate recovered from a patient with diarrhoea. | a qnrs2 gene was identified in an aeromonas caviae isolate (mics of ciprofloxacin, norfloxacin and ofloxacin >32 mg/l) from a stool sample collected from a patient with gastroenteritis. the analysis of the gyra and parc genes revealed amino acid substitutions ser83-ile and ser80-thr, respectively. in addition, five out of 41 nalidixic acid-resistant aeromonas isolates studied (26 identified as aeromonas veronii bv sobria and 15 identified as a. caviae) showed ciprofloxacin resistance. the identi ... | 2010 | 19863591 |
| mechanisms of quinolone resistance in aeromonas species isolated from humans, water and eels. | mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species aeromonas hydrophila, aeromonas caviae, aeromonas media, aeromonas salmonicida, aeromonas popoffii and aeromonas veronii, recovered from humans, freshwater and eels. the quinolone resistance-determining regions (qrdrs) of gyra and parc genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive aeromonas used as controls. all quinolone-resistant aeromonas carried point mutations in ... | 2010 | 19900538 |
| aeromonas caviae cb101 contains four chitinases encoded by a single gene chi1. | aeromonas caviae cb101 secretes four chitinases (around 92, 82, 70, and 55 kda) into the culture supernatant. a chitinase gene chi1 (92 kda) was previously studied. to identify the genes encoding the remaining three chitinases, a cosmid library of cb101 was constructed to screen for putative chitinase genes. nine cosmid clones were shown to contain a chitinase gene on chitin plates. surprisingly, all the positive clones contained chi1. in parallel, we purified the 55-kda chitinase (chi55) from t ... | 2010 | 19960373 |
| diversity of extracellular proteases among aeromonas determined by zymogram analysis. | the current research was aimed at comparing extracellular proteolytic activities and zymogram profiles among aeromonas spp. | 2010 | 20059617 |
| aeromonas caviae strain induces th1 cytokine response in mouse intestinal tract. | aeromonas caviae has been associated with human gastrointestinal disease. strains of this species typically lack virulence factors (vfs) such as enterotoxins and hemolysins that are produced by other human pathogens of the aeromonas genus. microarray profiling of murine small intestinal extracts, 24 h after oral infection with an a. caviae strain, provides evidence of a th1 type immune response. a large number of gamma-interferon (gamma-ifn) induced genes are up-regulated as well as several tumo ... | 2010 | 20130691 |
| emergence of ctx-m-3, tem-1 and a new plasmid-mediated mox-4 ampc in a multiresistant aeromonas caviae isolate from a patient with pneumonia. | aeromonas species rarely cause pulmonary infection. we report, for what is believed to be the first time, a case of severe pneumonia in a cancer patient caused by aeromonas caviae. detailed microbiological investigation revealed that this isolate carried three beta-lactamase-encoding genes (encoding mox-4, ctx-m-3 and tem-1) conferring resistance to all beta-lactams but imipenem. the beta-lactamase with a pi of 9.0 was transferred by conjugation and associated with a 7.3 kb plasmid, as demonstra ... | 2010 | 20339022 |
| [cloning and molecular organization of the polyhydroxyalkanoic acid synthase gene (phac) of ralstonia eutropha strain b5786]. | class i polyhydroxyalkanoic acid (pha) synthase gene (phac) of ralstonia eutropha strain b5786 was cloned and characterized. r. eutropha b5786 features the ability to synthesize multicomponent phas with short- and medium-chain-length monomers from simple carbohydrate substrate. a correlation was made between the molecular structure of pha synthase and substrate specificity and the ability of strain-producers to accumulate phas of this or that structure. a strong similarity of pha synthase of r. ... | 2010 | 20391757 |
| comonomer compositional distribution, physical properties, and enzymatic degradability of bacterial poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) copolyesters. | poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) (p(3hb-co-3h4mv)) was synthesized by using ralstonia eutropha strain phb(-)4 (pha-negative mutant) harboring with the n149s/d171g double mutation of pha synthase gene from aeromonas caviae (phac(ac) nsdg mutant) with 4-methylvaleric acid and fructose as the carbon sources. because the microbially synthesized p(3hb-co-3h4mv) samples were found to have broad comonomer compositional distribution, p(3hb-co-3h4mv) samples with 3h4mv content of 7, ... | 2010 | 20441146 |
| validation of a partial rpob gene sequence as a tool for phylogenetic identification of aeromonads isolated from environmental sources. | a collection of 50 aeromonads isolated from environmental sources were studied, together with all known aeromonas nomenspecies, by phenotypic, amplified 16s rdna restriction analysis (16s rdna rflp) and by partial sequence alignment of both 16s rdna and rpob genes. although most of the type strain showed a unique phenotypic pattern, a database constructed on type strain phenotype allowed the identification of only 24% of the isolates. analysis of 16s rdna rflp and the rpob sequence were almost c ... | 2010 | 20453908 |
| necrotising fasciitis in both calves caused by aeromonas caviae following aesthetic liposuction. | liposuction is the most widely performed cosmetic procedure in the world, and is considered safe and without serious complications. however, necrotising fasciitis has been documented as a rare complication following abdomen and thigh liposuction. we present a case of necrotising fasciitis in a 22-year-old female who underwent cosmetic liposuction in both calves. the diagnosis of necrotising fasciitis was delayed, leading to multi-organ dysfunction and skin necrosis with consequent massive skin l ... | 2010 | 20471929 |