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effects of moraxella bovis and culture filtrates on 51cr-labeled bovine neutrophils.the cytotoxicity of moraxella bovis whole cells and culture filtrates was studied, using 51cr-labeled bovine and human blood neutrophils. the cytotoxicity of living m bovis was directly related to the concentration of bacteria in the neutrophil cultures, and was maximal at an approximate neutrophil to bacteria ratio of 1:10. cytotoxicity was maximal by 30 minutes after living bacteria were added to the suspension of the 51cr-labeled neutrophils. expression of the cytotoxicity was dependent on th ...19892919823
investigation of an outbreak of moraxella conjunctivitis at a navajo boarding school.in 1986, an outbreak of moraxella follicular conjunctivitis occurred in girls attending a navajo boarding school in new mexico. we diagnosed 19 cases of culture-proven, and 21 of clinical conjunctivitis based on isolation of moraxella from conjunctival cultures and the occurrence of symptoms significantly associated with positive culture. sharing eye makeup was significantly associated with moraxella-positive conjunctivitis (odds ratio [or] = 7.2, p = .004) and showed a trend toward significance ...19892929704
identification of eikenella corrodens and cardiobacterium hominis by genetic transformation.genetic transformation was employed to attain exact identification of eikenella corrodens and cardiobacterium hominis. the two species appeared both homogeneous and without genetic affinity to each other or to species of kingella, neisseria, and moraxella, as tested for by rather sensitive procedures with streptomycin- and spectinomycin-resistance markers.19852939687
in vitro activity of ciprofloxacin against aminoglycoside-sensitive and -resistant gram-negative rods.ciprofloxacin's activity against 233 clinically isolated strains of both fermentative and non-fermentative bacilli was tested. in particular the authors studied the sensitivity of aminoglycoside-resistant gram-negative bacilli to ciprofloxacin. the results showed that all fermentative gram-negative bacteria were sensitive to ciprofloxacin (mics range less than or equal to 0.02 - 1.56 mg/l). among the non-fermentative bacilli, pseudomonas were generally susceptible to ciprofloxacin (mic90 less th ...19862941174
comparative in vitro activity of a-56268 against respiratory tract pathogens. 19872959474
purification of mbo ii methylase (gaagma) from moraxella bovis: site specific cleavage of dna at nine and ten base pair sequences.the restriction modification methylase m. mbo ii has been purified using a sensitive oligonucleotide linker assay. the enzyme methylates the mbo ii recognition sequence* gaaga at adenine to produce gaagma. m. mbo ii can be used in conjunction with the methylation dependent restriction endonuclease dpn i (gmatc) to produce cleavage at the 10 base sequence gaagatcttc. when m. mbo ii is used in combination with m. cla i (atcgatcgat), cleavage by dpn i occurs at the four ten base sequences gaagatctt ...19852997742
[treatment of peritonitis in continuous ambulatory peritoneal dialysis with intraperitoneal ceftriaxone].in 16 patients under capd, 29 cases of bacterial peritonitis were observed, with clinical manifestations in 23. the mean cell count in peritoneal dialysis fluid was 5608/mm3 with 4991/mm3 polymorphonuclear, leukocytes causative pathogens were staphylococcus in 14 cases, streptococcus in 6, bacillus in one, enterobacteria in 5, pseudomonas aeruginosa in 1 and moraxella in 1. three cultures were negative. first choice treatment was a daily intraperitoneal injection of 1 g of ceftriaxone. 79.3% of ...19883043356
[species-forming involution of pathogenic bacteria as a biological pattern (exemplified by bacteria of the genus moraxella)].the author advances a hypothesis stating that a species disappearing as a result of new conditions evolving in a colonized animal or human body, unfavorable for the existence of this species, does not become extinct, but reverts into newly developing species due to the loss of its properties and acquiring new properties corresponding to new conditions. thus the genus is preserved through the loss of species characteristics by individual organisms. the advanced hypothesis is substantiated by the ...19883061260
bacterial keratitis after penetrating keratoplasty.bacterial keratitis continues to be a serious problem in developing countries. the authors studied 881 patients who had undergone penetrating keratoplasty (total of 947 procedures) from january 1983 to march 1986 at the king khaled eye specialist hospital, riyadh, saudia arabia. all patients were followed for at least 6 months. clinical evidence of bacterial keratitis developed in 113 (11.9%) eyes with penetrating keratoplasties in 108 patients. the causative organisms among those patients inclu ...19883062524
increase in bronchopulmonary infection due to branhamella catarrhalis.in a six month prospective study during the winter branhamella catarrhalis was isolated from the sputum of 63 patients with symptoms of bronchopulmonary infection: 49 isolates were in pure culture and 14 were with another pathogen, haemophilus influenzae being the commonest (found with 10 of the 14 b catarrhalis isolates). of 36 patients infected in the community, 26 required admission to hospital. the remaining 27 patients were infected while in hospital. forty four of the 63 isolates produced ...19863084017
in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics.a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ...19863088346
automation of microbial enumeration: development of a disposable hydrophobic grid-membrane filter unit.a disposable filter unit containing a hydrophobic grid-membrane filter (hgmf) was developed. the unit is liquid tight to serve as a specimen transport container and, by removal of the funnel extender (175- or 300-ml capacity), the unit becomes less than the height of two stacked petri plates to save space during in situ incubation. the polyethylene mesh which supports the hgmf facilitates rinse removal of any substance(s) that would interfere with microbial growth. the correlations between a pou ...19863096206
antimicrobial activity of cefmetazole (cs-1170) and recommendations for susceptibility testing by disk diffusion, dilution, and anaerobic methods.cefmetazole, formerly cs-1170, was found to have antimicrobial activity slightly superior to that of cefoxitin but a clinically usable antimicrobial spectrum that should be considered identical to that of cefoxitin. disk diffusion and dilution test methods with cefmetazole correlated highly (r, greater than or equal to 0.95) with cefoxitin results. the recommended 30-micrograms cefmetazole disk interpretive breakpoints for susceptibility and resistance were greater than or equal to 18 mm (mic, l ...19863097064
bacterial colonization and occurrence of legionella pneumophila in warm and cold water, in faucet aerators, and in drains of hospitals.warm and cold water as well as water from wash basin drains and faucet aerators was examined to determine the number of viable and dead bacteria by culture and by staining and to establish the spectrum of species with special consideration of legionella pneumophila. the relation between the number of legionella pneumophila, the temperature, and the iron content of the water was determined in three separate warm water systems. high colony counts (up to 8.9 x 10(5) colony-forming units), were dete ...19863107260
supplement peptone agar--a simple carbohydrate degradation plate medium for the identification of neisseria species.a carbohydrate degradation medium was developed for the detection of acid production by neisseria species and branhamella catarrhalis. a total of 223 clinical isolates were identified by supplemented peptone agar and the results were compared with those of cystine trypticase agar. supplemented peptone agar and cystine trypticase agar correctly identified 99.1% and 93.7% of the total strains respectively within 24 h. with cystine trypticase agar method another 4% of the isolates could be identifi ...19873115001
murine monoclonal antibodies for detection of antigens and culture identification of neisseria meningitidis group b and escherichia coli k-1.four murine monoclonal antibodies which reacted with a (2----8)alpha-linked sialic acid polysaccharide were produced. three of the antibodies reacted specifically with neisseria meningitidis serogroup b and escherichia coli k-1 polysaccharide antigens, whereas one antibody cross-reacted with n. meningitidis group c polysaccharide antigen, a (2----9)alpha-linked homopolymer of sialic acid. by using the most avid antibody (mb 62), a latex particle agglutination test was developed which could detec ...19873116036
the influence of culture medium on the comparative in-vitro activity of amoxycillin and amoxycillin/clavulanic acid against branhamella catarrhalis. 19873117763
an analysis of the organization and evolution of type 4 fimbrial (mephe) subunit proteins.we have analyzed and compared the amino acid sequences of the type 4 fimbrial subunits from pseudomonas aeruginosa. moraxella bovis, m. nonliquefaciens, bacteroides nodosus, neisseria gonorrhoeae, and n. meningitidis. we propose a consensus sequence for the highly conserved amino-terminal regions of these proteins. in the variable regions, a domain corresponding to an epitope common to n. gonorrhoeae and n. meningitidis fimbriae is conserved, both in sequence and in environment, in fimbrial subu ...19873118043
respiratory tract infections due to branhamella catarrhalis: epidemiological data from western australia.during a 3-year period branhamella catarrhalis was isolated in significant numbers from 239 (1.3%) of 19,488 specimens of sputum sent for routine microbiological examination at a 700-bed general hospital. the majority of patients (83%) were over 60 years of age and 65% were male. there was a distinct seasonal variation in isolations with a peak incidence during the winter and early spring, a pattern not found with other pathogens. susceptibility to amoxycillin decreased by approximately 50% over ...19873119360
correlation between branhamella catarrhalis adherence to oropharyngeal cells and seasonal incidence of lower respiratory tract infections.bacterial colonization of the oropharynx is the initial event in most lower respiratory tract infections. to study the role of bacterial adherence in lower respiratory tract infections caused by branhamella catarrhalis (b. catarrhalis) in winter, in vitro adherence assays of the organism to human oropharyngeal cells were carried out in winter, spring, summer and autumn. a total of 57 adults of both sexes were studied from january to december 1985. forty eight persons of 57 had chronic pulmonary ...19873120353
secondary bacterial keratitis in herpes zoster ophthalmicus.we report a case of an unusual complication of herpes zoster ophthalmicus, secondary bacterial keratitis. compared with previously reported cases, ours is unique in its early occurrence in the course of zoster and the lack of predisposing factors such as steroid use, contact lens use, or prior corneal disease or surgery. the opportunistic pathogen branhamella cattarhalis responded well to medical therapy. we feel that bacterial superinfection must always be a concern in patients with herpes zost ...19873121255
rapid identification of pathogenic neisseria species and branhamella catarrhalis.two systems, the identicult-neisseria (idn; scott laboratories, inc., fiskeville, r.i.) strip and the neisseria/haemophilus identification test kit (nhi; vitek systems, inc., hazelwood, mo.) card, were compared with the 4-h minitek system (bbl microbiology systems, cockeysville, md.) for their ability to rapidly identify 157 pathogenic neisseria and branhamella catarrhalis isolates. idn, limited in its identification to four species, when incubated at 35 degrees c for 10 min identified 99% of th ...19873121668
branhamella catarrhalis as a cause of bacterial tracheitis. 19873122157
branhamella catarrhalis and streptococcus pneumoniae type 9 causing recurrent coalescent mastoiditis. 19873122159
branhamella catarrhalis respiratory infections.branhamella catarrhalis, a normal commensal of the oropharynx, is increasingly recognized as an important cause of bronchitis and bacterial pneumonia. six patients with b. catarrhalis pneumonia documented by transtracheal aspirate or blood culture were studied, and 429 previously reported cases of b. catarrhalis bronchitis and pneumonia were reviewed. the mean age of patients with b. catarrhalis infection was 64.8 years, and preexisting chronic obstructive pulmonary disease was common. the typic ...19873122301
branhamella catarrhalis: beta-lactamase production and sensitivity to oral antibiotics, including new cephalosporins. 19873123451
disk diffusion susceptibility testing of branhamella catarrhalis with ampicillin and seven other antimicrobial agents.a total of 74 clinical isolates of branhamella catarrhalis were characterized with respect to their ampicillin, amoxicillin-clavulanate, cephalothin, cefaclor, erythromycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole mics and zones of inhibition. disk diffusion tests were performed according to the guidelines of the national committee for clinical laboratory standards with two different media (mueller-hinton agar and chocolate mueller-hinton agar) and plates incubated under ...19873124732
middle ear fluid bacteriology of acute otitis media in neonates and very young infants.the middle ear fluid (mef) bacteriology of 107 attacks of acute otitis media (aom) in 101 infants less than 3 months old was analyzed. a total of 108 bacteria were isolated from 85 attacks. major aom-pathogens, s. pneumoniae (19%), h. influenzae (9%) or b. catarrhalis (7%) were cultured in approximately one-third of all the attacks. s. aureus (17%) and coagulase-negative staphylococci (22%) without the above pathogens were commonly found, whereas gram-negative enteric bacteria were culturable fr ...19873125118
branhamella catarrhalis as a possible skin pathogen. 19873128412
selective medium with dnase test agar and a modified toluidine blue o technique for primary isolation of branhamella catarrhalis in sputum.a selective medium with dnase test agar and incorporating vancomycin (10 micrograms/ml), trimethoprim (8 micrograms/ml), and amphotericin b (2 micrograms/ml) supported the growth of 305 branhamella catarrhalis isolates. a modified toluidine blue o technique was used after 48 h of incubation in co2 to overlay suspected b. catarrhalis colonies. a metachromatic color change was observed in 15 min, indicating dnase production. in 200 unselected sputum samples of hospitalized patients, this method wa ...19883128575
evaluation of eight methods for identification of pathogenic neisseria species: neisseria-kwik, rim-n, gonobio-test, minitek, gonochek ii, gonogen, phadebact monoclonal gc omni test, and syva microtrak test.the performance of eight methods in identifying neisseria species, particularly n. gonorrhoeae, was evaluated. these methods included four rapid carbohydrate utilization tests (gonobio-test, neisseria-kwik, rim-n, and minitek); the gonochek ii, a test which is based on the utilization of chromogenic substrates; and three monoclonal antibody tests (syva microtrak, gonogen, and phadebact monoclonal gc omni test). in all, 182 isolates comprised in six species of neisseria as well as branhamella cat ...19883128578
antimicrobial resistance in branhamella catarrhalis. 19883129387
enoxacin in acute exacerbations of chronic bronchitis: a comparison with amoxycillin.a total of 43 hospitalized adult patients with acute exacerbations of chronic bronchitis or bronchiectasis due to gram-negative bacteria were randomized to receive either enoxacin (400 mg bd) or amoxycillin (1,000 mg tid) for 7-12 days. micro-organisms isolated included 24 haemophilus influenzae (three beta-lactamases positive), 11 branhamella catarrhalis (six beta-lactamase positive), two pseudomonas aeruginosa and two neisseria meningitidis in 37 evaluable patients. in the enoxacin group (23 p ...19883129390
immunoglobulin g (igg) serological response to branhamella catarrhalis in patients with acute bronchopulmonary infections.over 12 months serum was collected from 45 inpatients, with acute bronchopulmonary infection, in whose sputum branhamella catarrhalis predominated, or was the sole pathogen. serum was examined for igg against b catarrhalis using an immunofluorescence antibody test. acute and convalescent sera were compared with sera of age and sex matched controls. the convalescent sera had significantly higher titres than the acute sera which in turn had higher titres than the controls. the findings confirm the ...19883129454
newer oral antimicrobials and newer etiologic agents of acute bronchitis and acute exacerbations of chronic bronchitis.although the role of antimicrobial therapy in the treatment of chronic bronchitis is unproven, physicians continue to look for microbial etiologies to explain episodes of clinical acute bronchitis and better antimicrobial agents with which to treat these episodes. the newest major pathogen of acute bronchitis is branhamella catarrhalis, a neisseriae-like organism that has become the third most commonly recognized cause of this disease after haemophilus influenzae and streptococcus pneumoniae. be ...19883129770
in vitro activities of 39 antimicrobial agents for branhamella catarrhalis and comparison of results with different quantitative susceptibility test methods.the in vitro activities of 39 antimicrobial agents were assessed versus 74 clinical isolates of branhamella catarrhalis. resistance was observed only with penicillin and ampicillin and then only with beta-lactamase-producing strains. the results of in vitro susceptibility tests with agar dilution and broth microdilution procedures were found to be comparable. the results of broth tube macrodilution tests were, in general, one twofold-concentration increment higher.19883129988
in vitro susceptibilities of common pediatric pathogens to ly163892.ly163892 is a carbacephem antibiotic for oral administration with an antibacterial spectrum similar to that of cefaclor and amoxicillin-clavulanic acid. it has greater stability than cefaclor and greater activity against beta-lactamase-producing haemophilus influenzae and escherichia coli. ly163892 is less active than amoxicillin against streptococci and less active than amoxicillin-clavulanic acid against branhamella catarrhalis but comparable against other pathogens.19883129989
emergence and persistence of beta-lactamase-producing bacteria in the oropharynx following penicillin treatment.the emergence and persistence of aerobic and anaerobic beta-lactamase-producing bacteria (blpb) were investigated in 26 children treated with penicillin for otitis media or pharyngitis and in 28 nontreated control children. beta-lactamase-producers were isolated in three (12%) of the treated children before therapy, in 12 (46%) seven to ten days after completion of therapy, in nine (35%) 40 to 45 days after therapy, and in seven (27%) 85 to 90 days after therapy. these organisms were present in ...19883130087
sputum microbiology in a district general hospital. the role of branhamella catarrhalis.evidence is presented that supports the view that branhamella catarrhalis has a pathogenic role in chronic chest disease, similar in many respects to haemophilus influenzae. it was isolated from 4% of sputum specimens (compared to 10% for haemophilus influenzae). fifty-three per cent produced beta-lactamase, and all were resistant to trimethoprim. the relevance of these findings to antibiotic treatment is discussed.19873130089
branhamella catarrhalis septicemia in patients with leukemia.during a 10-year period, four patients with leukemia were identified who had branhamella catarrhalis septicemia. two patients had acute leukemia and the remaining two had chronic myelogenous leukemia with blastic transformation. all patients were febrile and neutropenic at the onset of the septicemia. after appropriate antibiotic therapy, they recovered from their infection despite persistence of neutropenia. because beta-lactamase-producing bacteria are an increasing cause of nosocomial infecti ...19883130177
proposed changes in interpretive criteria and potency of ampicillin and ampicillin-sulbactam disks for susceptibility tests.the accuracy of disk susceptibility tests with ampicillin and ampicillin-sulbactam was not improved when the amount of ampicillin was increased from 10 to 20 or 30 micrograms per disk. for testing members of the family enterobacteriaceae, ampicillin disk tests correlated better with broth microdilution tests when the zone size standards were altered from greater than or equal to 14 greater than or equal to 17 mm for susceptible and from less than or equal to 11 to less than or equal to 13 mm for ...19883130390
evaluation of a rapid method for identifying branhamella catarrhalis.the speed and precision of hydrolysis of tributyrin to butyric acid as a test to detect branhamella catarrhalis were evaluated. the test proved consistently reliable in the identification of strains and correctly differentiated b catarrhalis from neisseria sp. the combination of gram stain, oxidase, and catalase tests to tributyrin hydrolysis provides a means of positive same day identification of b catarrhalis.19883130412
[acute laryngobronchitis by moraxella subgenus branhamella catarrhalis (infantile case report)]. 19873130436
[infective endocarditis due to kingella kingae in a patient with systemic lupus erythematosus]. 19873130447
beta-lactamase interference with antibiotic activity. 19883130608
comparison of the microbiology of group a and non-group a streptococcal tonsillitis.we studied the microbial flora of tonsils removed from 20 children who suffered from recurrent group a beta-hemolytic streptococcal (gabhs) tonsillitis and 20 who had tonsillar hypertrophy following recurrent non-gabhs tonsillitis. similar polymicrobial aerobic and anaerobic flora were recovered from the cores of the tonsils in each group. beta-lactamase-producing bacteria (blpb) were recovered more often in the group with gabhs. this difference was due mostly to the lower incidence of beta-lact ...19883132074
evaluation of a ten-minute chromogenic substrate test for identification of pathogenic neisseria species and branhamella catarrhalis.a ten-minute chromogenic substrate test was evaluated for its ability to rapidly identify pathogenic neisseria spp. and branhamella catarrhalis. identifications obtained with this system were compared to those obtained using conventional procedures. the test correctly identified 98.9% of 90 neisseria gonorrhoeae, 98.3% of 60 neisseria meningitidis, 96.2% of 26 neisseria lactamica, and 100% of 36 branhamella catarrhalis strains. eight neisseria subflava strains that grew on modified thayer-martin ...19883132371
comparative antimicrobial activity of the new macrolide flurithromycin against respiratory pathogens.the activity of flurithromycin against haemophilus influenzae, streptococcus pneumoniae, streptococcus pyogenes, branhamella catarrhalis and staphylococcus aureus was determined by the agar dilution method. flurithromycin showed high activity against streptococcus pneumoniae, streptococcus pyogenes and branhamella catarrhalis (mic90 = 0.032-0.25 mg/l). its mic90 value against haemophilus influenzae strains was 4.0 mg/l and 16 mg/l against staphylococcus aureus strains. flurithromycin has promisi ...19883132384
the effects of sinus bacteria on human ciliated nasal epithelium in vitro.the mechanisms by which bacteria colonize and damage ciliated epithelium are important in understanding the pathophysiology of rhinitis, sinusitis, and otitis. bacteria that have the ability to impair mucociliary clearance would be at an advantage in establishing infection of ciliated surfaces. this study investigates the effect of hemophilus influenzae, streptococcus pneumoniae, branhamella catarrhalis, and staphylococcus epidermidis on the ciliary activity of normal ciliated nasal epithelium i ...19883132682
endotoxin and otitis media. 19883132888
[branhamella catarrhalis infection]. 19883133094
rapid identification of branhamella catarrhalis with 4-methylumbelliferyl butyrate.branhamella catarrhalis can be distinguished from neisseria spp. by the presence of butyrate esterase. this enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. all b. catarrhalis strains tested gave a positive fluorescence reaction within 5 min, while neisseria spp. remained negative, even after 18 h of incubation.19883133392
profiles of amino-acid utilisation and production amongst strains of branhamella catarrhalis.utilisation and production of amino acids by isolates of branhamella catarrhalis was studied by ion exchange chromatography after cells had been grown in nutrient broth and mueller-hinton broth. the profiles of amino acids used and produced by each strain were compared by a single linkage cluster algorithm. the results of this study reflect the biochemical and physiological heterogeneity amongst strains of b. catarrhalis.19883133481
kingella kingae osteomyelitis: report of two cases involving the epiphysis. 19883134646
[branhamella catarrhalis: incidence in pulmonary infections and determination of sensitivity to 5 antibiotics].this study reports 45 cases of respiratory tract infection associated with branhamella catarrhalis, diagnosed by bacteriological examination out of 980 sputum samples studied over a 6 months period. these infections were observed mainly in patients with chronic respiratory disease (68.9%). more than half of the isolates were found in pure culture, others were isolated from mixed infections most often with haemophilus influenzae, streptococcus pneumoniae or h. influenzae plus s. pneumoniae. 64.7% ...19883136431
evaluation of three commercial systems for the identification of pathogenic neisseria and branhamella species against the conventional method.we compared three commercial systems for the identification of neisseria and branhamella spp. with the conventional method using cystine-tryptic digest agar (cta) supplemented with carbohydrates, dnase production, and nitrate reduction. we evaluated the api quadferm+ [( api], analytab products, inc., plainview, n.y.), neisseria [( pasteur], diagnostics pasteur, marnes-la-coquette, france), and neisseria identification discs [( oxoid], oxoid ltd., basingstoke, england) using the conventional meth ...19883136606
normal aerobic bacterial flora of throat in the population of dhaka city. 19873136762
bactericidal activity of bmy-28100 versus amoxicillin/clavulanic acid against branhamella catarrhalis. 19883137055
in vitro activity of ly163892 against pathogens isolated from pediatric patients. 19883137056
[respiratory infections caused by branhamella catarrhalis: clinical analysis concerning its pathogenicity]. 19883137299
adherence of branhamella catarrhalis to human pharyngeal cells: its role in the pathogenesis of lower respiratory infection. 19883138342
the epidemiology of respiratory tract pathogens in southern netherlands.results of sputum cultures and susceptibility tests have been reviewed for the years 1977-1986, with approximately 1,700 positive cultures per year. haemophilus influenzae always occupied the first place, being grown in 65% of positive specimens in 1977 and 37% in 1986. streptococcus pneumoniae occupied the second place, rising from 17 to 27% in the same period, during which branhamella catarrhalis rose from 5 to 26%. based on the frequency of occurrence of individual bacterial species in the sp ...19883139445
laboratory diagnosis of branhamella catarrhalis. 19883139719
comparison of the outer membrane proteins of 50 strains of branhamella catarrhalis.branhamella catarrhalis colonizes the respiratory tract of humans and commonly causes otitis media in children and respiratory infections in adults with chronic lung disease. in view of the emergence of this organism as an important human pathogen, we used sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) to examine the outer membrane proteins (omps) of 50 strains of b. catarrhalis. omps were isolated from broth culture supernatants. typical of other gram-negative bacteria, ei ...19883139777
branhamella catarrhalis sepsis: a case report and review of the literature. 19883139780
recurrent and penicillin v-resistant otitis media. a treatment study with amoxycillin/clavulanate and cefaclor.clinical and bacteriological studies were performed in 122 children, aged 1-10 years, with recurrent otitis media or failed therapy with phenoxymethyl penicillin. a specimen for bacteriological culture was taken from the nasopharynx in all patients, and in our material haemophilus influenzae, branhamella catarrhalis and streptococcus pneumoniae were equally distributed, each forming one-third of the total bacterial count. thus, h. influenzae and b. catarrhalis (as well as their betalactamase-pro ...19883140574
branhamella catarrhalis meningitis following otolaryngologic surgery.a case of pyogenic meningitis caused by branhamella catarrhalis which occurred after pharyngoplasty, adenoidectomy and tympanostomy tube placement is reported. this organism is morphologically similar to neisseria meningitidis but often produces beta lactamase. therefore therapy should be directed by proper microbiologic identification and sensitivity results.19883140851
efficacy of penicillin treatment in purulent maxillary sinusitis. a european multicenter trial.three hundred and one patients with maxillary sinusitis participated in a double-blind, randomized study at 11 ent-clinics in europe. sinusitis was diagnosed by the presence of at least two signs and symptoms and sinus x-ray showing more than 6 mm swelling of the maxillary mucosa. a microbiological specimen was obtained by intrasinusal aspiration. the patients were randomly assigned to treatment either with bacampillin 800 mg b. i. d. or with amoxicillin 500 mg t. i. d. for ten days. the most fr ...19883141290
in-vitro antimicrobial activity of 7432-s (sch39720) against commonly isolated respiratory tract pathogens. 19883141348
spiramycin: in-vitro activity on branhamella catarrhalis.this in-vitro study of susceptibility to spiramycin of 103 strains of branhamella catarrhalis isolated between 1982 and 1987 was performed by evaluation of their mics. more than 97% of strains remained susceptible with mic less than or equal to 8 mg/l (two strains). one strain presented a mic of 16 mg/l. there were no significant differences of susceptibility to spiramycin between penicillinase-producing and non-producing strains.19883141349
[in vitro activity of 3 fluoroquinolones on branhamella catarrhalis].the in vitro antibacterial activities of three fluoroquinolones (pefloxacin, ofloxacin, ciprofloxacin) against 90 clinical isolates of branhamella catarrhalis were assessed by determination of minimum inhibitory concentrations (mics). 73.5% of the strains were producing penicillinase. the mics90 were as follow: ciprofloxacin = 0.2 mg/l. the mic50 and the mic90 were similar. there was no correlation of mics with beta-lactam resistance. lung parenchyma and bronchial mucus diffusion of these three ...19883141885
branhamella catarrhalis bacteremia in children. 19883141896
beta-lactamase-producing branhamella in beijing, china. 19883141897
differentiation of some gram-negative glucose nonfermenting bacteria using miniaturized carbon sources assimilation tests.in water and soil the gram-negative nonfermenting bacteria play an important role in the biological mineralization process. to improve the methods for species differentiation of these heterogenous bacterial group, a total of 481 reference strains of gram-negative glucose nonfermenting bacteria belonging to the genera pseudomonas, alcaligenes, bordetella, agrobacterium, moraxella, acinetobacter, flavobacterium and some cdc groups have been investigated for their ability to utilize 42 different ca ...19883142164
plasma bactericidal activity after administration of erythromycin estolate and erythromycin ethylsuccinate to healthy volunteers.in a crossover design study, we compared the plasma bactericidal activities of erythromycin estolate (500 mg) and erythromycin ethylsuccinate (600 mg) after administration of a single oral dose to 12 healthy volunteers. both erythromycin esters displayed very good plasma bactericidal activities against streptococcus pneumoniae. the median bactericidal titers produced in plasma against streptococcus pyogenes and streptococcus pneumoniae were significantly higher with erythromycin estolate than wi ...19883142349
branhamella keratitis resistant to penicillin and cephalosporins. case report. 19883142449
acute laryngitis in adults: diagnosis, etiology, treatment. 19883144113
secretory otitis media--infectious background and its implications for treatment. 19883144115
rate of bactericidal activity for branhamella catarrhalis of fleroxacin compared with that of amoxycillin-clavulanic acid.the rate of bactericidal activity of fleroxacin was compared with that of the combination of amoxycillin and clavulanic acid (in the proportion of 4 to 1) on beta-lactamase producing strains of branhamella catarrhalis. the rate of bactericidal activity of 1 mg/l was as rapid as that of 1 mg/l of amoxycillin-clavulanic acid combination. this rate was not significantly more rapid if the concentrations of fleroxacin were increased to 10 mg/l. in conclusion, the bactericidal activity of fleroxacin i ...19883144548
the antibiotic susceptibilities and beta-lactamase production of clinical isolated branhamella catarrhalis from acute otitis media in children.branhamella catarrhalis has been misconsidered as a normal resident in human respiratory tract for a long time. however, many authors recently have reported its pathogenecity and isolated it from the otolaryngological region. in our study, this organism can be isolated from the ear and nasal discharge in the child with acute otitis media by the rate of 7.5% and 21.4% respectively. out of this 107 isolated strains, 97 strains (90.7%) were found to be beta-lactamase producing organisms. the mic me ...19883144965
branhamella catarrhalis septicemia in an infant with aids.branhamella catarrhalis was isolated in blood culture from a 6-month-old infant with aids. the child had a nasopharyngeal infection with respiratory syncytial virus and pneumonitis with pneumocystis carinii. commensal organisms such as b. catarrhalis may be potential pathogens in infants with aids.19883146808
pneumonia due to branhamella catarrhalis.in 12 of 451 patients diagnosed as having pneumonia in a single hospital over 18 months the causative organism appeared to be branhamella catarrhalis.19883146819
in vitro evaluation of ceftibuten (7432-s, sch 39720), a novel orally administered cephalosporin.7432-s (sch 39720) was the most active beta-lactam tested against the enterobacteriaceae, inhibiting 92% of strains at less than or equal to 8.0 micrograms/ml compared to 82%, 65% and 39% of strains inhibited by cefixime, cefuroxime and cefaclor, respectively. 7432-s was also very effective against haemophilus influenzae (mic90, less than or equal to 0.25 microgram/ml), branhamella catarrhalis (mic90, 4.0 micrograms/ml) and neisseria meningitidis (mic90, less than or equal to 0.25 microgram/ml). ...19883147148
branhamella catarrhalis: fatty acid and lipopolysaccharide analysis of an atypical strain from blood culture.a branhamella catarrhalis-like organism was isolated from blood cultures; it was atypical in showing negative nitrate and nitrite and positive gamma-glutamylaminopeptidase reactions, in agglutinating with neisseria meningitidis antisera, and in forming butyrous colonies. cell-wall fatty-acid and lipopolysaccharide analysis provided evidence that this isolate was an atypical b. catarrhalis.19883147160
selective medium for branhamella catarrhalis with acetazolamide as a specific inhibitor of neisseria spp.several semiselective media for branhamella catarrhalis have been proposed. these media allow growth of all members of the family neisseriaceae, and further differentiation is necessary. by addition of 10 micrograms of acetazolamide, a carbonic anhydrase inhibitor, per ml and incubation in air, a medium was created which reduced growth of neisseria spp. when saliva samples from 178 healthy schoolchildren were screened for the presence of b. catarrhalis, the carrier rate for this organism was est ...19883147992
effect of autogenic bacterial antigens on the production of histamine releasing factor by mononuclear cells from intrinsic asthmatic patients.the production of histamine releasing factor (hrf) by mononuclear cells (mnc) from intrinsic asthmatic patients has previously been reported. in this study, we investigated the effect of preincubation of lymphocytes with autogenic killed bacteria upon the production of hrf. bacteria were isolated from the sputum, and nasopharyngeal swab obtained from patients and control subjects. mnc from intrinsic asthmatics and healthy controls were preincubated with killed bacteria for 4 h, then washed and c ...19883148281
purification and properties of cytochrome p-450 from moraxella sp.a cytochrome p-450 has been purified to homogeneity from a moraxella species that is able to grow on guaiacol as the sole source of carbon and energy. the pure cytochrome was a monomeric protein of about 52 kda, with no catalytic activity towards guaiacol. the difference in mm extinction coefficients between 450 and 490 nm in the co-difference spectrum was 89.5 mm-1.cm-1. the typical shift of the soret band from 415 to 390 nm that is attributed to the high-spin state of the cytochrome was observ ...19883148326
[changing pattern of respiratory pathogens due to the development of antibacterial agents]. 19883148770
characterization of a plasmid isolated from branhamella catarrhalis and detection of plasmid sequences within the genome of a b. catarrhalis strain.we isolated a 12.2-kb plasmid from two clinical strains of branhamella catarrhalis and evaluated its distribution among other b. catarrhalis strains by colony hybridization experiments using the whole plasmid as a probe. homology was detected with the two plasmid-bearing strains and also with a third b. catarrhalis strain named e7, which is plasmidless. southern transfer analysis of total digested e7 dna using the purified plasmid as a probe revealed a single band of hybridization, different fro ...19883148947
neisseria meningitidis and moraxella osloensis: dual infection in blood and peritoneal fluid.the clinical course of a malnourished alcoholic in which neisseria meningitidis was isolated from the blood and moraxella osloensis from the peritoneal fluid is described. following bacteriologic diagnosis, the patient was treated and responded to a course of penicillin therapy. to our knowledge, this represents the first case of peritonitis associated with m. osloensis. clinical reports of the isolation of this organism are rare; its pathogenicity is not clearly established, and the presence of ...19883149224
[recent trend of incidence of respiratory pathogenic bacteria and its susceptibility to antimicrobial agents: studies in the year 1984-1986].based on a quantitative analysis of sputum cultures, pathogenic bacteria in respiratory ailments isolated in our laboratory during 1984 to 1986 were classified and analyzed. during the study period, the most frequently isolated agent was haemophilus influenzae followed by pseudomonas aeruginosa, branhamella catarrhalis and streptococcus pneumoniae. they together consisted of 70 approximately 74% of all the respiratory pathogenic bacteria isolated in our study. susceptibilities of above pathogens ...19883149325
in-vitro activity of amoxycillin/clavulanic acid and five other oral antibiotics against clinical isolates of haemophilus influenzae and branhamella catarrhalis.the in-vitro activity of amoxycillin/clavulanic acid, amoxycillin, erythromycin, doxycycline, chloramphenicol and cephalexin against 183 clinical isolates of haemophilus influenzae and 61 of branhamella catarrhalis was determined. amoxycillin/clavulanic acid showed the greatest activity against h. influenzae. all isolates of b. catarrhalis were susceptible to amoxycillin/clavulanic acid, chloramphenicol, cephalexin and doxycycline.19883149634
[branhamella catarrhalis and bronchopulmonary infection]. 19883149788
antimicrobial susceptibility testing of haemophilus influenzae, branhamella catarrhalis, and neisseria gonorrhoeae. 19883149883
respiratory tract infections due to branhamella catarrhalis and neisseria species. 19873151758
in vitro antibacterial activity of norfloxacin and other agents against ocular pathogens.302 clinical isolates representing 16 bacterial species most often implicated in ocular infections were tested in vitro against norfloxacin and a panel of antibacterial agents. on the basis of the 90% minimal inhibitory concentration (mic90) data, norfloxacin was 4-32 times more active than the next best antimicrobial tested against citrobacter freundii, escherichia coli, morganella morganii, proteus mirabilis, proteus vulgaris, haemophilus influenzae, neisseria gonorrhoeae and staphylococcus ep ...19853157551
moraxella ovis in cases of infectious bovine keratoconjunctivitis (ibk) in israel. 19883188722
kingella kingae bacteremia and adult epiglottitis in a granulocytopenic host. 19883189372
[aerobic and aero-anaerobic bacterial flora of the nasal cavities of lambs].on the basis of bacteriological examinations carried out on 415 intranasal swabs, aerobic and aero-anaerobic respiratory microbes were studied in lambs. a great diversity of bacteria has been found (in total 35 bacterial species and genus have been identified). this flora is characterised by the presence of gram positive cocci with a gram negative strain included in genus moraxella and connected with moraxella bovis species.19883190138
comparative activities of cefuroxime, amoxicillin-clavulanic acid, ciprofloxacin, enoxacin, and ofloxacin against aerobic and anaerobic bacteria isolated from bite wounds.we studied the comparative in vitro activities of 10 oral antimicrobial agents against 147 aerobic and 61 anaerobic bacteria making up species in 13 genera (staphylococcus aureus, streptococci, eikenella corrodens, pasteurella multocida, haemophilus-actinobacillus spp., m-5, ef-4, moraxella spp., flavobacterium iib, bacteroides melaninogenicus, bacteroides spp., fusobacterium spp., and peptostreptococcus spp.) that were isolated from bite wounds. cefuroxime was generally greater than fourfold mo ...19883190202
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