Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| signature proteins that are distinctive of alpha proteobacteria. | the alpha (alpha) proteobacteria, a very large and diverse group, are presently characterized solely on the basis of 16s rrna trees, with no known molecular characteristic that is unique to this group. the genomes of three alpha-proteobacteria, rickettsia prowazekii (rp), caulobacter crescentus (cc) and bartonella quintana (bq), were analyzed in order to search for proteins that are unique to this group. | 2005 | 15960851 |
| predictive value of clinical and microbiological parameters for the treatment outcome of scaling and root planing. | to compare the clinical and microbiological outcome of non-surgical periodontal therapy after 6 months with data obtained after hygienic phase or 6 weeks after completion of non-surgical therapy, in order to evaluate the value of clinical and microbiological parameters to predict treatment success. | 2005 | 15966873 |
| association between coinfection of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and treponema denticola and periodontal tissue destruction in chronic periodontitis. | the association between the infection of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and treponema denticola in chronic periodontitis (cp) and the severity of periodontal disease remains to be elucidated. the aim of this study was to investigate the subgingival infection frequencies of three periodontopathic bacteria in chinese cp patients and to evaluate the correlations between infection by these bacteria and periodontal destruction. | 2005 | 15978192 |
| diagnostic biomarkers for oral and periodontal diseases. | this article provides an overview of periodontal disease diagnosis that uses clinical parameters and biomarkers of the disease process.this article discusses the use of biomarkers of disease that can be identified at the tissue, cellular, and molecular levels and that are measurable in oral fluids such as saliva and gingival crevicular fluid. biomarkers identified from these biologic fluids include microbial, host response, and connective tissue-related molecules that can target specific pathway ... | 2005 | 15978241 |
| the bioinformatics resource for oral pathogens. | complete genomic sequences of several oral pathogens have been deciphered and multiple sources of independently annotated data are available for the same genomes. different gene identification schemes and functional annotation methods used in these databases present a challenge for cross-referencing and the efficient use of the data. the bioinformatics resource for oral pathogens (brop) aims to integrate bioinformatics data from multiple sources for easy comparison, analysis and data-mining thro ... | 2005 | 15980574 |
| periodontal disease and coronary heart disease: a reappraisal of the exposure. | results from studies relating periodontal disease to cardiovascular disease have been mixed. residual confounding by smoking and use of clinical measures of periodontal disease rather than measures of infection have been 2 major criticisms. the aims of this study were to investigate relationships between prevalent coronary heart disease (chd) and 2 exposures, (1) clinical periodontal disease and (2) igg antibodies to 17 oral organisms, and to evaluate the role of smoking in these relationships. | 2005 | 15983248 |
| the salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects. | the purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (oscc) lesion would differ from those found in cancer-free (oscc-free) controls. | 2005 | 15987522 |
| a leptospira interrogans enzyme with similarity to yeast ste14p that methylates the 1-phosphate group of lipid a. | distinct from other spirochetes, cells of leptospira interrogans contain orthologues of all the escherichia coli lpx genes required for lipid a biosynthesis, but they synthesize a modified form of lipopolysaccharide that supposedly activates toll-like receptor 2 (tlr2) instead of tlr4. the recent determination of the l. interrogans lipid a structure revealed an unprecedented o-methylation of its 1-phosphate group (que-gewirth, n. l. s., ribeiro, a. a., kalb, s. r., cotter, r. j., bulach, d. m., ... | 2005 | 15994324 |
| prevalence of putative periodontopathogens in subgingival dental plaques from gingivitis lesions in korean orthodontic patients. | the objective of this study was to detect and compare the presence of periodontopathogens in the subgingival plaques of gingivitis lesions in adults who wore fixed orthodontic appliances, as opposed to adults who did not wear any orthodontic appliances. thirty-six individuals participated in this study. nineteen of these subjects did not wear any orthodontic appliances, and these subjects comprised the control group. the other 17 individuals had been wearing fixed orthodontic appliances for at l ... | 2005 | 15995644 |
| uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. | endodontic infections have been traditionally studied by culture methods, but recent reports showing that over 50% of the oral microbiota is still uncultivable (b. j. paster et al., j. bacteriol. 183:3770-3783, 2001) raise the possibility that many endodontic pathogens remain unknown. this study intended to investigate the prevalence of several uncultivated oral phylotypes, as well as newly named species in primary or persistent endodontic infections associated with chronic periradicular disease ... | 2005 | 16000454 |
| oral bacteria in the occluded arteries of patients with buerger disease. | recent studies have suggested that infectious organisms play a role in vascular diseases. in this study, to explore a possible link between oral infection and buerger disease, we investigated whether oral (periodontal) bacteria were present in occluded arteries removed from patients with characteristic buerger disease. | 2005 | 16012459 |
| origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new members. | we report an in-depth computational study of the protein sequences and structures of the superfamily of archaeo-eukaryotic primases (aeps). this analysis greatly expands the range of diversity of the aeps and reveals the unique active site shared by all members of this superfamily. in particular, it is shown that eukaryotic nucleo-cytoplasmic large dna viruses, including poxviruses, asfarviruses, iridoviruses, phycodnaviruses and the mimivirus, encode aeps of a distinct family, which also includ ... | 2005 | 16027112 |
| selective capture of salmonella enterica serovar typhi genes expressed in macrophages that are absent from the salmonella enterica serovar typhimurium genome. | thirty-six salmonella enterica serovar typhi-specific genes, absent from the salmonella enterica serovar typhimurium genome, that were expressed in human macrophages were identified by selective capture of transcribed sequences. these genes are located on 15 unique loci of the serovar typhi genome, including salmonella pathogenicity islands (spi-7, spi-8, and spi-10) and bacteriophages (st15, st18, and st35). | 2005 | 16041043 |
| antimicrobial peptides in the oral environment: expression and function in health and disease. | the oral cavity is a unique environment in which antimicrobial peptides play a key role in maintaining health and may have future therapeutic applications. present evidence suggests that alpha-defensins, beta-defensins, ll-37, histatin, and other antimicrobial peptides and proteins have distinct but overlapping roles in maintaining oral health and preventing bacterial, fungal, and viral adherence and infection. the expression of the inducible hbd-2 in normal oral epithelium, in contrast to other ... | 2005 | 16053246 |
| the leptospira interrogans lexa gene is not autoregulated. | footprinting and mutagenesis experiments demonstrated that leptospira interrogans lexa binds the palindrome tttgn(5)caaa found in the reca promoter but not in the lexa promoter. in silico analysis revealed that none of the other canonical sos genes is under direct control of lexa, making the leptospiral lexa gene the first described which is not autoregulated. | 2005 | 16077133 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| statistical analyses of complex denaturing gradient gel electrophoresis profiles. | studies using molecular techniques have demonstrated that a culture-based approach can severely underestimate the bacterial diversity in most environments. one of the molecular techniques that has been applied in microbial ecology is denaturing gradient gel electrophoresis (dgge). the purpose of this study was to investigate differences in the microbiota of plaque, using a number of analysis techniques, from children without gingivitis (n = 30) and from those with gingivitis (n = 30). extracted ... | 2005 | 16081938 |
| synergistic biofilm formation by treponema denticola and porphyromonas gingivalis. | biofilm formation is an important step in the etiology of periodontal diseases. in this study, in vitro biofilm formation by treponema denticola and porphyromonas gingivalis 381 displayed synergistic effects. confocal microscopy demonstrated that p. gingivalis attaches to the substratum first as a primary colonizer followed by coaggregation with t. denticola to form a mixed biofilm. the t. denticola flagella mutant as well as the cytoplasmic filament mutant were shown to be essential for biofilm ... | 2005 | 16085371 |
| evolution of selenium utilization traits. | the essential trace element selenium is used in a wide variety of biological processes. selenocysteine (sec), the 21st amino acid, is co-translationally incorporated into a restricted set of proteins. it is encoded by an uga codon with the help of trnasec (selc), sec-specific elongation factor (selb) and a cis-acting mrna structure (secis element). in addition, sec synthase (sela) and selenophosphate synthetase (seld) are involved in the biosynthesis of sec on the trnasec. selenium is also found ... | 2005 | 16086848 |
| detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in italian patients. | the presence of selected bacteria (enterococcus faecalis, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, treponema denticola) in infected root canals was studied using polymerase chain reaction (pcr) assays, and the association of bacteria with clinical signs of endodontic disease was assessed. the null hypothesis, that no difference could be observed between clinical signs of apical periodontitis and a specific bacterial strain, was tested. | 2005 | 16101964 |
| impact of smoking on the clinical, microbiological and immunological parameters of adult patients with periodontitis. | the aim of the current study was to assess the impact of smoking on the clinical indices, the humoral immune response and the detection frequency of putative periodontal pathogens in patients with periodontitis cross-sectionally and following therapy. | 2005 | 16104962 |
| identification of genes of vsh-1, a prophage-like gene transfer agent of brachyspira hyodysenteriae. | vsh-1 is a mitomycin c-inducible prophage of the anaerobic spirochete brachyspira hyodysenteriae. purified vsh-1 virions are noninfectious, contain random 7.5-kb fragments of the bacterial genome, and mediate generalized transduction of b. hyodysenteriae cells. in order to identify and sequence genes of this novel gene transfer agent (gta), proteins associated either with vsh-1 capsids or with tails were purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the n-terminal amino ... | 2005 | 16109929 |
| gene organization and transcriptional analysis of the tprj, tpri, tprg, and tprf loci in treponema pallidum strains nichols and sea 81-4. | the tpr gene family of treponema pallidum subsp. pallidum, the causative agent of syphilis, has recently become the focus of intensive investigation. tprf and tpri sequences are highly conserved among different isolates and are the targets of strong humoral and cellular immune responses of the host, and immunization with a recombinant peptide from the amino terminus of these antigens has been shown to alter significantly lesion development following homologous challenge. this indicates that thes ... | 2005 | 16109950 |
| horizontally acquired genes for purine salvage in borrelia spp. causing relapsing fever. | unlike borrelia burgdorferi, the relapsing fever agent borrelia hermsii and the related borrelia miyamotoi had pura and purb genes of the purine salvage pathway. these were located among the rrna genes. phylogenetic analysis indicated that these genes had a different evolutionary history than those of orthologs in other spirochetes. | 2005 | 16113341 |
| discrimination of streptococcus pneumoniae from viridans group streptococci by genomic subtractive hybridization. | two oligonucleotide primer sets for the discrimination of streptococcus pneumoniae from "pneumococcus-like" oral streptococcal isolates by pcr were developed. genomic subtractive hybridization was performed to search for differences between streptococcus pneumoniae strain wu2 and the most closely related oral streptococcus, streptococcus mitis strain 903. we identified 19 clones that contained s. pneumoniae-specific nucleotide fragments that were absent from the chromosomal dna of typical labora ... | 2005 | 16145102 |
| amylomaltase of pyrobaculum aerophilum im2 produces thermoreversible starch gels. | amylomaltases are 4-alpha-glucanotransferases (ec 2.4.1.25) of glycoside hydrolase family 77 that transfer alpha-1,4-linked glucans to another acceptor, which can be the 4-oh group of an alpha-1,4-linked glucan or glucose. the amylomaltase-encoding gene (pae1209) from the hyperthermophilic archaeon pyrobaculum aerophilum im2 was cloned and expressed in escherichia coli, and the gene product (pyamase) was characterized. pyamase displays optimal activity at ph 6.7 and 95 degrees c and is the most ... | 2005 | 16151092 |
| inflammatory responses of a macrophage/epithelial cell co-culture model to mono and mixed infections with porphyromonas gingivalis, treponema denticola, and tannerella forsythia. | accumulated evidence points to porphyromonas gingivalis, treponema denticola, and tannerella forsythia as three major etiologic agents of chronic periodontitis. epithelial cells and macrophages play a major role in the host response to periodontopathogens, and the secretion of inflammatory mediators and matrix metalloproteinases (mmps) by these host cells is believed to contribute to periodontal tissue destruction. the aim of this study was to investigate the inflammatory response of a macrophag ... | 2006 | 16153871 |
| quantitative analysis of multi-species oral biofilms by taqman real-time pcr. | oral infectious diseases, including dental caries, various forms of periodontitis and oral malodor, are not caused by a single pathogen. the etiology of these diseases is known to be associated with bacterial accumulation and plaque composition on the hard and soft tissues of the oral cavity. therefore, the quantitative, as well as qualitative, analysis of the microorganisms present in oral biofilms, namely dental plaque, subgingival plaque and tongue debris, is important for diagnosis and ratio ... | 2005 | 16160072 |
| a computational method to predict genetically encoded rare amino acids in proteins. | in several natural settings, the standard genetic code is expanded to incorporate two additional amino acids with distinct functionality, selenocysteine and pyrrolysine. these rare amino acids can be overlooked inadvertently, however, as they arise by recoding at certain stop codons. we report a method for such recoding prediction from genomic data, using read-through similarity evaluation. a survey across a set of microbial genomes identifies almost all the known cases as well as a number of no ... | 2005 | 16168086 |
| homologues of insecticidal toxin complex genes in yersinia enterocolitica biotype 1a and their contribution to virulence. | yersinia enterocolitica is an enteric pathogen that consists of six biotypes: 1a, 1b, 2, 3, 4, and 5. strains of the latter five biotypes can carry a virulence plasmid, known as pyv, and several well-characterized chromosomally encoded virulence determinants. y. enterocolitica strains of biotype 1a lack the virulence-associated markers of pyv-bearing strains and were once considered to be avirulent. there is growing epidemiological, clinical, and experimental evidence, however, to suggest that s ... | 2005 | 16177365 |
| evaluation of liaison treponema screen, a novel recombinant antigen-based chemiluminescence immunoassay for laboratory diagnosis of syphilis. | the purpose of this study was to evaluate the diagnostic performance of liaison treponema screen (diasorin, saluggia, italy), a new automated chemiluminescence immunoassay (clia), in comparison with that of rapid plasma reagin (rpr) and the following currently used treponemal tests: hemagglutination test (tpha), immunoenzymatic assay (eia), and western blot (wb). first, a retrospective study was performed with a panel of 2,494 blood donor sera, a panel of 131 clinical and serologically character ... | 2005 | 16210488 |
| probing the role of tyr 64 of treponema denticola cystalysin by site-directed mutagenesis and kinetic studies. | tyr 64, hydrogen-bonded to coenzyme phosphate in treponema denticola cystalysin, was changed to alanine by site-directed mutagenesis. spectroscopic and kinetic properties of the tyr 64 mutant were investigated in an effort to explore the differences in coenzyme structure and kinetic mechanism relative to those of the wild-type enzyme. the wild type displays coenzyme absorbance bands at 418 and 320 nm, previously attributed to ketoenamine and substituted aldamine, respectively. the tyr 64 mutant ... | 2005 | 16229486 |
| demonstration of factor h-like protein 1 binding to treponema denticola, a pathogen associated with periodontal disease in humans. | treponema denticola is an important contributor to periodontal disease. in this study we investigated the ability of t. denticola to bind the complement regulatory proteins factor h and factor h-like protein 1 (fhl-1). the binding of these proteins has been demonstrated to facilitate evasion of the alternative complement cascade and/or to play a role in adherence and invasion. here we demonstrate that t. denticola specifically binds fhl-1 via a 14-kda, surface-exposed protein that we designated ... | 2005 | 16239506 |
| defining the interaction of the treponema pallidum adhesin tp0751 with laminin. | various invasive pathogens attach to host tissues via the extracellular matrix component laminin, the major glycoprotein found within basement membranes. previous investigations identified the laminin-binding adhesin tp0751 within the spirochete bacterium treponema pallidum. in the current study, tp0751 was shown to attach to a variety of laminin isoforms that are widely distributed throughout the host, including laminins 1, 2, 4, 8, and 10. such universal attachment is conducive for an adhesin ... | 2005 | 16239550 |
| treponema denticola infection is not a cause of false positive treponema pallidum serology. | it has long been assumed that parodontal disease can be a cause of false positive results in syphilis serology, but so far there are no definitive data supporting this hypothesis. in this study we tested 250 serum samples obtained from blood donors. all of them were negative when routinely screened for antibodies against treponema pallidum. then, all these samples were tested by immunoenzymatic (elisa) and western blot (wb) assays to investigate reactivities against t. denticola. thirteen sample ... | 2005 | 16240693 |
| dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks. | bacterial response to nitric oxide (no) is of major importance since no is an obligatory intermediate of the nitrogen cycle. transcriptional regulation of the dissimilatory nitric oxides metabolism in bacteria is diverse and involves fnr-like transcription factors hcpr, dnr, and nnrr; two-component systems narxl and narqp; no-responsive activator norr; and nitrite-sensitive repressor nsrr. using comparative genomics approaches, we predict dna-binding motifs for these transcriptional factors and ... | 2005 | 16261196 |
| distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers. | we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ... | 2006 | 16269128 |
| defining the normal bacterial flora of the oral cavity. | more than 700 bacterial species or phylotypes, of which over 50% have not been cultivated, have been detected in the oral cavity. our purposes were (i) to utilize culture-independent molecular techniques to extend our knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-cultivated bacteria species, and (ii) to determine the site and subject specificity of bacterial colonization. nine sites from five clinically healthy subjects were analyzed. sites i ... | 2005 | 16272510 |
| biofilm formation by the periodontopathic bacteria treponema denticola and porphyromonas gingivalis. | periodontitis develops as a result of the interaction of the host with subgingival plaque bacteria. both porphyromonas gingivalis and treponema denticola are frequently associated together in these oral biofilms. | 2005 | 16277575 |
| involvement of periodontopathic anaerobes in aspiration pneumonia. | increasing evidence has linked the anaerobic bacteria forming periodontopathic biofilms with aspiration pneumonia in elderly persons. in experiments designed to eliminate the potent respiratory pathogens forming biofilms in the oral cavity, we have shown that the mechanical and chemical oral cleansing using povidone-iodine effectively reduced the detection rates and numbers of methicillin-sensitive staphylococcus species, streptococcus pneumoniae, and haemophilus influenzae in patients scheduled ... | 2005 | 16277588 |
| chex is a phosphorylated chey phosphatase essential for borrelia burgdorferi chemotaxis. | motility and chemotaxis are believed to be important in the pathogenesis of lyme disease caused by the spirochete borrelia burgdorferi. controlling the phosphorylation state of chey, a response regulator protein, is essential for regulating bacterial chemotaxis and motility. rapid dephosphorylation of phosphorylated chey (chey-p) is crucial for cells to respond to environmental changes. chey-p dephosphorylation is accomplished by one or more phosphatases in different species, including chez, che ... | 2005 | 16291669 |
| streptococci dominate the diverse flora within buccal cells. | previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ... | 2005 | 16304448 |
| evaluation of gel-pad oligonucleotide microarray technology by using artificial neural networks. | past studies have suggested that thermal dissociation analysis of nucleic acids hybridized to dna microarrays would improve discrimination among duplex types by scanning through a broad range of stringency conditions. to more fully constrain the utility of this approach using a previously described gel-pad microarray format, artificial neural networks (nns) were trained to recognize noisy or low-quality data, as might derive from nonspecific fluorescence, poor hybridization, or compromised data ... | 2005 | 16332861 |
| identification and localization of extraradicular biofilm-forming bacteria associated with refractory endodontic pathogens. | bacterial biofilms have been found to develop on root surfaces outside the apical foramen and be associated with refractory periapical periodontitis. however, it is unknown which bacterial species form extraradicular biofilms. the present study aimed to investigate the identity and localization of bacteria in human extraradicular biofilms. twenty extraradicular biofilms, used to identify bacteria using a pcr-based 16s rrna gene assay, and seven root-tips, used to observe immunohistochemical loca ... | 2005 | 16332869 |
| comparative genomic analyses of the bacterial phosphotransferase system. | we report analyses of 202 fully sequenced genomes for homologues of known protein constituents of the bacterial phosphoenolpyruvate-dependent phosphotransferase system (pts). these included 174 bacterial, 19 archaeal, and 9 eukaryotic genomes. homologues of pts proteins were not identified in archaea or eukaryotes, showing that the horizontal transfer of genes encoding pts proteins has not occurred between the three domains of life. of the 174 bacterial genomes (136 bacterial species) analyzed, ... | 2005 | 16339738 |
| the 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism. | the 2-hydroxycarboxylate transporter family is a family of secondary transporters found exclusively in the bacterial kingdom. they function in the metabolism of the di- and tricarboxylates malate and citrate, mostly in fermentative pathways involving decarboxylation of malate or oxaloacetate. these pathways are found in the class bacillales of the low-cg gram-positive bacteria and in the gamma subdivision of the proteobacteria. the pathways have evolved into a remarkable diversity in terms of th ... | 2005 | 16339740 |
| prospective study of the long term change of the oral flora after radiation therapy. | the aim of this prospective study was to evaluate the long term change in oral pathogens following radiation therapy. | 2006 | 16341728 |
| [experience in medical ozone use for root canal treatment]. | the results of clinical and laboratory assessment of effectiveness of psr-diagnosis are presented. the high efficacy of ozone therapy is revealed. the findings demonstrate the reduction of number in the micro-organisms in root canal: actinobacillus actinomycetemcomitans from 31.25% to 10.21%; bacteriodes forsythus from 68.75% to 15.50%; treponema denticola from 37.5% to 11.4%; porphyromonas gingivalis from 56.25% to 45%; prevotella intermedia from 16% to 0%. | 2005 | 16353031 |
| characterization, distribution, and expression of novel genes among eight clinical isolates of streptococcus pneumoniae. | eight low-passage-number streptococcus pneumoniae clinical isolates, each of a different serotype and a different multilocus sequence type, were obtained from pediatric participants in a pneumococcal vaccine trial. comparative genomic analyses were performed with these strains and two s. pneumoniae reference strains. individual genomic libraries were constructed for each of the eight clinical isolates, with an average insert size of approximately 1 kb. a total of 73,728 clones were picked for ar ... | 2006 | 16368987 |
| fusobacterium nucleatum transports noninvasive streptococcus cristatus into human epithelial cells. | analysis of human buccal epithelial cells frequently reveals an intracellular polymicrobial consortium of bacteria. although several oral bacteria have been demonstrated to invade cultured epithelial cells, several others appear unable to internalize. we hypothesized that normally noninvasive bacteria may gain entry into epithelial cells via adhesion to invasive bacteria. fusobacterium nucleatum is capable of binding to and invading oral epithelial cells. by contrast, streptococcus cristatus bin ... | 2006 | 16369022 |
| lipoprotein computational prediction in spirochaetal genomes. | lipoproteins are of great interest in understanding the molecular pathogenesis of spirochaetes. because spirochaete lipobox sequences exhibit more plasticity than those of other bacteria, application of existing prediction algorithms to emerging sequence data has been problematic. in this paper a novel lipoprotein prediction algorithm is described, designated splip, constructed as a hybrid of a lipobox weight matrix approach supplemented by a set of lipoprotein signal peptide rules allowing for ... | 2006 | 16385121 |
| relationship of periodontal bacteria and porphyromonas gingivalis fima variations with phenytoin-induced gingival overgrowth. | we investigated the relationship between phenytoin-induced gingival overgrowth (go) and the harboring of periodontal bacteria. | 2006 | 16390469 |
| [microbiological study on the prognosis of immediate implant and periodontal disease]. | the aim of the present study was to investigate the prevalence of periodontal marker organisms and specific interleukin-1 (il-1) gene polymorphisms (which show a close association with periodontitis) and their effect on the success of immediate implant placement postextraction in the patient with periodontal disease. | 2006 | 16395549 |
| recovery and evolutionary analysis of complete integron gene cassette arrays from vibrio. | integrons are genetic elements capable of the acquisition, rearrangement and expression of genes contained in gene cassettes. gene cassettes generally consist of a promoterless gene associated with a recombination site known as a 59-base element (59-be). multiple insertion events can lead to the assembly of large integron-associated cassette arrays. the most striking examples are found in vibrio, where such cassette arrays are widespread and can range from 30 kb to 150 kb. besides those found in ... | 2006 | 16417647 |
| biological basis for syphilis. | syphilis is a chronic sexually transmitted disease caused by treponema pallidum subsp. pallidum. clinical manifestations separate the disease into stages; late stages of disease are now uncommon compared to the preantibiotic era. t. pallidum has an unusually small genome and lacks genes that encode many metabolic functions and classical virulence factors. the organism is extremely sensitive to environmental conditions and has not been continuously cultivated in vitro. nonetheless, t. pallidum is ... | 2006 | 16418521 |
| regulation and physiologic significance of the agmatine deiminase system of streptococcus mutans ua159. | we previously demonstrated that streptococcus mutans expresses a functional agmatine deiminase system (agds) encoded by the agmatine-inducible agubdac operon (a. r. griswold, y. y. chen, and r. a. burne, j. bacteriol. 186:1902-1904, 2004). the agds yields ammonia, co2, and atp while converting agmatine to putrescine and is proposed to augment the acid resistance properties and pathogenic potential of s. mutans. to initiate a study of agu gene regulation, the agub transcription initiation site wa ... | 2006 | 16428386 |
| function and evolution of plasmid-borne genes for pyrimidine biosynthesis in borrelia spp. | the thyx gene for thymidylate synthase of the lyme borreliosis (lb) agent borrelia burgdorferi is located in a 54-kb linear plasmid. in the present study, we identified an orthologous thymidylate synthase gene in the relapsing fever (rf) agent borrelia hermsii, located it in a 180-kb linear plasmid, and demonstrated its expression. the functions of the b. hermsii and b. burgdorferi thyx gene products were evaluated both in vivo, by complementation of a thymidylate synthase-deficient escherichia ... | 2006 | 16428394 |
| the transmission of anaerobic periodontopathic organisms. | the oral microbial flora is unique, and available evidence indicates that it is passed vertically from parents to children. in this investigation, we used a chairside assay for the n-benzoyl-dl-arginine-2-naphthylamide (bana)-sensitive enzyme found in porphyromonas gingivalis, treponema denticola, and tannerella forsythensis, to determine the prevalence of these bana-positive species in young children and their caregivers. we predicted that if the bana enzyme was found in plaque samples of child ... | 2006 | 16434739 |
| the metabolism of cholesterol and certain hormonal steroids by treponema denticola. | the aim was to investigate whether reference cultures and fresh isolates of treponema denticola are able to 5alpha-reduce and further metabolise testosterone, 4-androstenedione, progesterone, corticosterone, cortisol or cholesterol. two reference and five freshly isolated cultures of t. denticola were incubated with either radiolabeled or unlabeled steroid substrates; in the first case products were identified by thin layer chromatography and in the latter by gas chromatography-mass spectroscopy ... | 2006 | 16436288 |
| supportive periodontal therapy using mechanical instrumentation or 2% minocycline gel: a 12 month randomized, controlled, single masked pilot study. | to compare the short-term performance of subgingival local delivery of 2% minocycline gel and conventional subgingival debridement in supportive periodontal therapy (spt) patients. | 2006 | 16441740 |
| comparison of bacterial plaque samples from titanium implant and tooth surfaces by different methods. | studies have shown similarities in the microflora between titanium implants or tooth sites when samples are taken by gingival crevicular fluid (gcf) sampling methods. the purpose of the present study was to study the microflora from curette and gcf samples using the checkerboard dna-dna hybridization method to assess the microflora of patients who had at least one oral osseo-integrated implant and who were otherwise dentate. plaque samples were taken from tooth/implant surfaces and from sulcular ... | 2006 | 16441779 |
| early colonization of non-submerged dental implants in patients with a history of advanced aggressive periodontitis. | the aim of the study was to evaluate the early colonization of non-submerged implants over a 6-month period in partially edentulous patients treated for advanced aggressive periodontal disease. in 22 patients treated for advanced aggressive periodontitis and in a supportive maintenance program for a period between 12 and 240 months at implant surgery, a total of 68 non-submerged dental implants were installed. patients had a plaque score below 20%, and less than 20% of the pockets around the tee ... | 2006 | 16441780 |
| inhibition of periodontopathogen-derived proteolytic enzymes by a high-molecular-weight fraction isolated from cranberry. | porphyromonas gingivalis, tannerella forsythia and treponema denticola are three major aetiological agents of chronic periodontitis. the strong proteolytic activities of these bacteria are critical to their survival since their energy source is obtained from peptides and amino acids derived from proteins. in addition, proteases are important factors contributing to periodontal tissue destruction by a variety of mechanisms, including direct tissue degradation and modulation of host inflammatory r ... | 2006 | 16473919 |
| algebraic comparison of metabolic networks, phylogenetic inference, and metabolic innovation. | comparison of metabolic networks is typically performed based on the organisms' enzyme contents. this approach disregards functional replacements as well as orthologies that are misannotated. direct comparison of the structure of metabolic networks can circumvent these problems. | 2006 | 16478540 |
| brucella abortus synthesizes phosphatidylcholine from choline provided by the host. | the brucella cell envelope is characterized by the presence of phosphatidylcholine (pc), a common phospholipid in eukaryotes that is rare in prokaryotes. studies on the composition of brucella abortus 2308 phospholipids revealed that the synthesis of pc depends on the presence of choline in the culture medium, suggesting that the methylation biosynthetic pathway is not functional. phospholipid composition of pmta and pcs mutants indicated that in brucella, pc synthesis occurs exclusively via the ... | 2006 | 16484204 |
| is pre-term labour associated with periodontitis in a danish maternity ward? | to reveal differences in periodontal status and presence of subgingival bacteria in a scandinavian population of women with pre-term birth compared with women who delivered at term. | 2006 | 16489943 |
| modulation of human neutrophil functions in vitro by treponema denticola major outer sheath protein. | in this study of human polymorphonuclear leukocytes (pmns), pretreatment with treponema denticola major outer sheath protein (msp) inhibited formyl-methionyl-leucyl-phenylalanine (fmlp)-induced chemotaxis, phagocytosis of immunoglobulin g-coated microspheres, fmlp-stimulated calcium transients, and actin assembly. msp neither altered oxidative responses to phorbol myristate or fmlp nor induced apoptosis. msp selectively impairs chemotaxis and phagocytosis by impacting the pmn cytoskeleton. | 2006 | 16495573 |
| selective binding of borrelia burgdorferi ospe paralogs to factor h and serum proteins from diverse animals: possible expansion of the role of ospe in lyme disease pathogenesis. | the binding of borrelia burgdorferi ospe, ospf, and family 163 (elp) proteins to factor h/factor h-like protein 1 (fhl-1) and other serum proteins from different animals was assessed. ospe paralogs bound factor h and unidentified serum proteins from a subset of animals, while ospf and elp proteins did not. these data advance our understanding of factor h binding, the host range of the lyme spirochetes, and the expanding role of ospe in pathogenesis. | 2006 | 16495576 |
| anti-inflammatory activity of a high-molecular-weight cranberry fraction on macrophages stimulated by lipopolysaccharides from periodontopathogens. | periodontitis is a chronic inflammatory disease affecting oral tissues. the continuous, high production of cytokines by host cells triggered by periodontopathogens is thought to be responsible for the destruction of tooth-supporting tissues. macrophages play a critical role in this host inflammatory response to periodontopathogens. the aim of this study was to investigate the effect of non-dialyzable material prepared from cranberry juice concentrate on the pro-inflammatory cytokine response of ... | 2006 | 16498070 |
| description of the subgingival microbiota of periodontally untreated mexican subjects: chronic periodontitis and periodontal health. | recent studies have suggested that changes in the prevalence and/or proportion of distinct microorganisms characterize the subgingival microbial profiles of populations around the world. at present, no information is available on the subgingival microbiota of mexican subjects. the purpose of the present study was to determine the microbial composition of subgingival plaque in mexican subjects with untreated chronic periodontitis. | 2006 | 16512761 |
| detection of bacterial dna in atheromatous plaques by quantitative pcr. | this is the first study to analyze atheromatous plaques for the presence of bacterial dna from ten species, including periodontal species and chlamydia pneumoniae. we examined 129 samples of dna extracted from atheromas from 29 individuals for the presence of bacterial 16s rdna sequences from ten different species: porphyromonas gingivalis, actinobacillus actinomycetemcomitans (a.a.), tannerella forsythensis, eikenella corrodens, prevotella intermedia, staphylococcus aureus, staphylococcus epide ... | 2006 | 16513386 |
| paths of lateral gene transfer of lysyl-aminoacyl-trna synthetases with a unique evolutionary transition stage of prokaryotes coding for class i and ii varieties by the same organisms. | while the premise that lateral gene transfer (lgt) is a dominant evolutionary force is still in considerable dispute, the case for widespread lgt in the family of aminoacyl-trna synthetases (aars) is no longer contentious. aarss are ancient enzymes, guarding the fidelity of the genetic code. they are clustered in two structurally unrelated classes. only lysine aminoacyl-trna synthetase (lysrs) is found both as a class 1 and a class 2 enzyme (lysrs1-2). remarkably, in several extant prokaryotes b ... | 2006 | 16529662 |
| periodontal infections and coronary heart disease: role of periodontal bacteria and importance of total pathogen burden in the coronary event and periodontal disease (corodont) study. | chronic inflammation from any source is associated with increased cardiovascular risk. periodontitis is a possible trigger of chronic inflammation. we investigated the possible association between periodontitis and coronary heart disease (chd), focusing on microbiological aspects. | 2006 | 16534043 |
| identification of a homozygous deletion in the ap3b1 gene causing hermansky-pudlak syndrome, type 2. | we report on the molecular etiology of an unusual clinical phenotype associating congenital neutropenia, thrombocytopenia, developmental delay, and hypopigmentation. using genetic linkage analysis and targeted gene sequencing, we defined a homozygous genomic deletion in ap3b1, the gene encoding the beta chain of the adaptor protein-3 (ap-3) complex. the mutation leads to in-frame skipping of exon 15 and thus perturbs proper assembly of the heterotetrameric ap-3 complex. consequently, trafficking ... | 2006 | 16537806 |
| clinical review: molecular mechanisms underlying the role of antithrombin in sepsis. | in disseminated intravascular coagulation (dic) there is extensive crosstalk between activation of inflammation and coagulation. endogenous anticoagulatory pathways are downregulated by inflammation, thus decreasing the natural anti-inflammatory mechanisms that these pathways possess. supportive strategies aimed at inhibiting activation of coagulation and inflammation may theoretically be justified and have been found to be beneficial in experimental and initial clinical studies. this review ass ... | 2006 | 16542481 |
| molecular analyses of the interaction of borrelia hermsii fhba with the complement regulatory proteins factor h and factor h-like protein 1. | borrelia hermsii, the primary etiological agent of tick-borne relapsing fever in north america, binds the complement regulatory protein factor h (fh) as a means of evading opsonophagocytosis and the alternative complement pathway. the ability of fh-binding protein a (fhba) to bind fh-like protein 1 (fhl-1) has not been assessed previously. in this study, using a whole-cell absorption assay, we demonstrated that b. hermsii absorbs both fh and fhl-1 from human serum. consistent with this, affinity ... | 2006 | 16552029 |
| exposure of lymphocytes to high doses of actinobacillus actinomycetemcomitans cytolethal distending toxin induces rapid onset of apoptosis-mediated dna fragmentation. | we have shown previously that treatment of human lymphocytes with the actinobacillus actinomycetemcomitans cytolethal distending toxin (cdt) results in dose-dependent g2 arrest, followed 24 h later by apoptotic cell death. here we demonstrated that for jurkat cells exposed to high concentrations of cdt (>0.2 ng/ml) there was a dose-dependent increase in the level of s-phase cells and a concomitant decrease in the level of g2 cells. fluorescence-activated cell sorter analysis demonstrated that th ... | 2006 | 16552037 |
| topical h2 antagonist prevents periodontitis in a rabbit model. | cimetidine is a powerful h2 receptor antagonist that eliminates histamine's effects on chemotaxis, phagocytosis, and superoxide anion production by phagocytes. the purpose of this study was to analyze the clinical and histopathological changes associated with experimental periodontitis in rabbits in response to topically applied cimetidine. experimental periodontitis was induced in 21 new zealand white rabbits using porphyromonas gingivalis (10(9) cfu) topically applied three times a week for a ... | 2006 | 16552070 |
| the treponema denticola surface protease dentilisin degrades interleukin-1 beta (il-1 beta), il-6, and tumor necrosis factor alpha. | dentilisin is a major surface protease and virulence factor of the bacterium treponema denticola. in this study, we found that t. denticola reduced inflammatory cytokines, including interleukin-1beta (il-1beta), il-6, and tumor necrosis factor alpha, in peripheral blood mononuclear cells through degradation by dentilisin. | 2006 | 16552080 |
| clinical and microbiological effects of different antimicrobials on generalized aggressive periodontitis. | to evaluate and compare the effects of adjunctive metronidazole plus amoxicillin, doxycycline and metronidazole on clinical and microbiological parameters in patients with generalized aggressive periodontitis. | 2006 | 16553634 |
| protease-activated receptor-2 activation: a major role in the pathogenesis of porphyromonas gingivalis infection. | we have investigated the specific contribution of protease-activated receptor-2 (par(2)) to host defense during porphyromonas gingivalis infection. culture supernatants from p. gingivalis strains 33277 and w50 provoked ca(2+) mobilization in cells transfected with par(2) (par(2)-knrk) and desensitized the subsequent responses to par(2)-selective agonist. in addition, culture supernatants of p. gingivalis e8 (rgpa/rgpb double knockout) did not cause calcium response in par(2)-knrk cells, evidenci ... | 2006 | 16565494 |
| subgingival and tongue microbiota during early periodontitis. | periodontal infections have a microbial etiology. association of species with early disease would be useful in determining which microbes initiate periodontitis. we hypothesized that the microbiota of subgingival and tongue samples would differ between early periodontitis and health. a cross-sectional evaluation of 141 healthy and early periodontitis adults was performed with the use of oligonucleotide probes and pcr. most species differed in associations with sample sites; most subgingival spec ... | 2006 | 16567551 |
| relationship of serum advanced glycation end products with deterioration of periodontitis in type 2 diabetes patients. | a close relationship between diabetes and chronic periodontitis has been demonstrated. we previously found that porphyromonas gingivalis with the type ii fima gene is an infectious factor closely associated with the deterioration seen in diabetic periodontitis patients. in the present study, we examined whether other biomarkers are related to the development and deterioration of periodontitis often seen in type 2 diabetic individuals. | 2006 | 16579698 |
| local oxygen therapy for treating acute necrotizing periodontal disease in smokers. | the main aim of treatment for acute necrotizing periodontal disease is fast and effective reduction of anaerobic destructive microorganisms to avoid periodontal damage. the effect of adjunctive local oxygen therapy in the treatment of necrotizing periodontal disease was examined in this study. | 2006 | 16579700 |
| langerhans' cell histiocytosis in a 5-year-old girl: evidence of periodontal pathogens. | langerhans' cell histiocytosis (lch) is a rare disorder characterized by langerhans' cell proliferation in various organs or tissues. when periodontal tissue is involved, clinical manifestations can vary from gingival recession and pocket formation to severe alveolar bone loss. this case report describes periodontal pathogens found in the pockets of involved primary teeth. | 2006 | 16584357 |
| identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions. | bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. this study determined the prevalence of a group of oral bacterial species on or in epithelial cells derived from periodontal pockets and the gingival crevice of subjects with periodontitis. samples of epithelial cells were obtained from 120 sites with periodontal pockets > or ... | 2006 | 16585650 |
| a database of bacterial lipoproteins (dolop) with functional assignments to predicted lipoproteins. | lipid modification of the n-terminal cys residue (n-acyl-s-diacylglyceryl-cys) has been found to be an essential, ubiquitous, and unique bacterial posttranslational modification. such a modification allows anchoring of even highly hydrophilic proteins to the membrane which carry out a variety of functions important for bacteria, including pathogenesis. hence, being able to identify such proteins is of great value. to this end, we have created a comprehensive database of bacterial lipoproteins, c ... | 2006 | 16585737 |
| the hemoglobin receptor protein of porphyromonas gingivalis inhibits receptor activator nf-kappab ligand-induced osteoclastogenesis from bone marrow macrophages. | extracellular proteinaceous factors of porphyromonas gingivalis, a periodontal pathogen, that influence receptor activator of nuclear factor-kappab (nf-kappab) ligand (rankl)-induced osteoclastogenesis from bone marrow macrophages were investigated. the culture supernatant of p. gingivalis had the ability to inhibit rankl-induced in vitro osteoclastogenesis. a major protein of the culture supernatant, hemoglobin receptor protein (hbr), suppressed rankl-induced osteoclastogenesis in a dose-depend ... | 2006 | 16622189 |
| inhibitory effects of macrocarpals on the biological activity of porphyromonas gingivalis and other periodontopathic bacteria. | macrocarpals, which are phloroglucinol derivatives contained in eucalyptus leaves, exhibit antimicrobial activity against a variety of bacteria including oral bacteria. this study examined effects of macrocarpals a, b, and c on periodontopathic bacteria, especially porphyromonas gingivalis. | 2006 | 16626372 |
| [quantitative analysis of periodontal pathogens in aggressive periodontitis patients in a japanese population]. | the aim of the present study was to clarify the relationship between the relative/absolute numbers of periodontal bacteria and different types of periodontitis. fifteen patients with localized aggressive periodontitis (lagp), 25 patients with generalized aggressive periodontitis (gagp) and 28 patients with chronic periodontitis (cp) were included in this study. saliva and subgingival plaque samples were collected from all subjects for microbiological analysis. the prevalence and proportions of a ... | 2006 | 16629473 |
| escherichia coli acid resistance: ph-sensing, activation by chloride and autoinhibition in gadb. | escherichia coli and other enterobacteria exploit the h+ -consuming reaction catalysed by glutamate decarboxylase to survive the stomach acidity before reaching the intestine. here we show that chloride, extremely abundant in gastric secretions, is an allosteric activator producing a 10-fold increase in the decarboxylase activity at ph 5.6. cooperativity and sensitivity to chloride were lost when the n-terminal 14 residues, involved in the formation of two triple-helix bundles, were deleted by m ... | 2006 | 16675957 |
| prevalence of periodontopathic bacteria on the tongue dorsum of elderly people. | the aim of this study was to analyse the prevalence of oral bacteria on the dorsum of the tongue. in addition, the relationship between the number of teeth and the microflora present on the coating of the tongue in a population of 85-year-old people was assessed. | 2006 | 16677187 |
| structural classification of bacterial response regulators: diversity of output domains and domain combinations. | chey-like phosphoacceptor (or receiver [rec]) domain is a common module in a variety of response regulators of the bacterial signal transduction systems. in this work, 4,610 response regulators, encoded in complete genomes of 200 bacterial and archaeal species, were identified and classified by their domain architectures. previously uncharacterized output domains were analyzed and, in some cases, assigned to known domain families. transcriptional regulators of the ompr, narl, and ntrc families w ... | 2006 | 16740923 |
| whole-genome transcription profiling reveals genes up-regulated by growth on fucose in the human gut bacterium "roseburia inulinivorans". | "roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. r. inulinivorans a2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing propionate and propanol as additional fermentation products. a shotgun genomic microarray was constructed and used to investigate the switch in gene expression that is involved in chan ... | 2006 | 16740940 |
| usefulness of real time pcr for the differentiation and quantification of 652 and jp2 actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva. | the aim of our study is to describe a fast molecular method, able to distinguish and quantize the two different genotypes (652 and jp2) of an important periodontal pathogen: actinobacillus actinomycetemcomitans. the two genotypes show differences in the expression of an important pathogenic factor: the leukotoxin (ltx). in order to evidence this, we performed a real time pcr procedure on the ltx operon, able to recognize aa clinical isolates with different leukotoxic potentials. | 2006 | 16772039 |
| comparative genomics and evolution of the hsp90 family of genes across all kingdoms of organisms. | hsp90 proteins are essential molecular chaperones involved in signal transduction, cell cycle control, stress management, and folding, degradation, and transport of proteins. hsp90 proteins have been found in a variety of organisms suggesting that they are ancient and conserved. in this study we investigate the nuclear genomes of 32 species across all kingdoms of organisms, and all sequences available in genbank, and address the diversity, evolution, gene structure, conservation and nomenclature ... | 2006 | 16780600 |
| t-cell expression cloning of porphyromonas gingivalis genes coding for t helper-biased immune responses during infection. | exposure of the mouse oral cavity to porphyromonas gingivalis results in the development of gingivitis and periapical bone loss, which apparently are associated with a th1 response to bacterial antigens. we have used this infection model in conjunction with direct t-cell expression cloning to identify bacterial antigens that induce a preferential or biased t helper response during the infectious process. a p. gingivalis-specific cd4 t-cell line derived from mice at 3 weeks postchallenge was used ... | 2006 | 16790769 |
| omega-3 fatty acid effect on alveolar bone loss in rats. | gingival inflammation and alveolar bone resorption are hallmarks of adult periodontitis, elicited in response to oral micro-organisms such as porphyromonas gingivalis. we hypothesized that omega (omega)-3 fatty acids (fa) dietary supplementation would modulate inflammatory reactions leading to periodontal disease in infected rats. rats were fed fish oil (omega-3 fa) or corn oil (n-6 fa) diets for 22 weeks and were infected with p. gingivalis. rats on the omega-3 fa diet exhibited elevated serum ... | 2006 | 16798867 |
| bacterial profile and burden of periodontal infection in subjects with a diagnosis of acute coronary syndrome. | periodontitis has been identified as a potential risk factor in cardiovascular diseases. it is possible that the stimulation of host responses to oral infections may result in vascular damage and the inducement of blood clotting. the aim of this study was to assess the role of periodontal infection and bacterial burden as an explanatory variable to the activation of the inflammatory process leading to acute coronary syndrome (acs). | 2006 | 16805672 |
| surface protease of treponema denticola hydrolyzes c3 and influences function of polymorphonuclear leukocytes. | treponema denticola is a dominant microorganism in human periodontal lesions. one of the major virulence factors of this microorganism is its chymotrypsin-like surface protease, dentilisin. the purpose of this study was to evaluate the effect of dentilisin on human polymorphonuclear leukocytes (pmns). we used chemiluminescence to assess production of o(-)(2) by pmns against t. denticola atcc 35405 and dentilisin-deficient mutant k1. t. denticola atcc 35405 induced production of o(-)(2), whereas ... | 2006 | 16815066 |