Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| monoreactive and polyreactive rheumatoid factors produced by in vitro epstein-barr virus-transformed peripheral blood and synovial b lymphocytes from rheumatoid arthritis patients. | the cd5 membrane molecule, initially identified as an exclusive t-cell marker, also defines a phenotypically and functionally distinct b-lymphocyte population. in normal individuals, cd5+ b cells are committed to secrete 'natural' polyreactive (auto)antibodies, that is antibodies, mainly igm, endowed with multiple antigen-binding capabilities, including rheumatoid factor (rf) activity. at variance with this, in rheumatoid arthritis (ra) as well as in other autoimmune conditions, monoreactive aut ... | 1990 | 1700467 |
| ligation of membrane immunoglobulin leads to inactivation of the signal-transducing ability of membrane immunoglobulin, cd19, cd21, and b-cell gp95. | we have examined the ability of membrane immunoglobulin-binding ligands to desensitize several human b-cell surface molecules that normally transduce signals leading to ca2+ mobilization. ligation of membrane igm or igd leads to heterologous desensitization of the reciprocal receptor in epstein-barr virus-transformed b-cell lines and peripheral blood b cells, as evidenced by a failure of cells to mobilize in response to receptor ligation. under these conditions cd19, cd21, and b-cell gp95 ligati ... | 1990 | 1701054 |
| long-term human b cell lines dependent on interleukin-4 and antibody to cd40. | cd40 is a 45- to 50-kilodalton transmembrane glycoprotein expressed on b lymphocytes, epithelial cells, and some carcinoma cell lines. human resting b lymphocytes entered a state of sustained proliferation when incubated with both the mouse fibroblastic ltk- cell line that had been transfected with the human fc receptor (fc gamma rii/cdw32) and monoclonal antibodies to cd40. in combination with interleukin-4, factor-dependent long-term normal human b cell lines were generated that were consisten ... | 1991 | 1702555 |
| complement alternative pathway activation and control on membranes of human lymphoid b cell lines. | membrane regulatory molecules normally prevent complement activation by autologous cells, therefore we compared the membrane control system of human lymphoid cell lines which activate or not human complement through the alternative pathway (ap). membrane expression of decay-accelerating factor (daf), membrane cofactor protein (mcp), complement receptors (cr)1, cr2 and h was measured either by radioimmunoassay or enzyme-linked immunosorbent assay on cell lysates. soluble extracts of isolated memb ... | 1990 | 1702720 |
| effect of fk 506 on epstein-barr virus specific cytotoxic t cells. | 1991 | 1703259 | |
| cd40 stimulation provides an ifn-gamma-independent and il-4-dependent differentiation signal directly to human b cells for ige production. | ige induction from human cells has generally been considered to be t cell dependent and to require at least two signals: il-4 stimulation and t cell/b cell interaction. in the present study we report a human system of t cell-independent ige production from highly purified b cells. when human cells were co-stimulated with a mab directed against cd40 (mab g28-5), there was induction of ige secretion from purified blood and tonsil b cells as well as unfractionated lymphocytes. anti-cd40 alone faile ... | 1991 | 1706382 |
| auto- and polyreactivity of igm from cd5+ and cd5- cord blood b cells. | the presence of the cd5 (67 kda) molecule on the surface of b cells has been considered a marker for cells producing auto- and polyreactive antibodies. cord blood b lymphocytes (rich in cd5+ b cells) have been sorted into cd5 positive and negative populations by flow cytometry using monoclonal antibodies to cd20 and cd5. clones of these populations were obtained by immortalization with epstein-barr virus. clones derived from both cd5+ and cd5- b cells produced igm which was auto- and polyreactiv ... | 1991 | 1707183 |
| expression of the interleukin 6 gene in rheumatoid synovial fibroblasts. | a number of fibroblastoid synovial cell lines have been established from rheumatoid joints. these cell lines were shown to express the interleukin 6 (il-6) gene constitutively, and exposure of these cells to 5 ng/ml of recombinant human interleukin 1 beta (il-1 beta) increased il-6 gene expression. other recombinant human lymphokines, namely interferon-gamma, tumor necrosis factor-alpha, and granulocyte-macrophage colony stimulating factor had no enhancing effect on il-6 gene expression. dexamet ... | 1990 | 1707461 |
| human cytotoxic t lymphocytes against the plasmodium falciparum circumsporozoite protein. | cytotoxic t lymphocytes (ctl) against the circumsporozoite (cs) protein of malaria sporozoites protect against malaria in rodents. although there is interest in developing human vaccines that induce ctl against the plasmodium falciparum cs protein, humans have never been shown to produce ctl against any plasmodium species protein or other parasite protein. we report that when peripheral blood mononuclear cells (pbmc) from three of four volunteers immunized with irradiated p. falciparum sporozoit ... | 1991 | 1707538 |
| an epstein-barr virus protein associated with cell growth transformation interacts with a tyrosine kinase. | epstein-barr virus (ebv) encodes two integral membrane proteins in latently infected growth-transformed cells. one of these, lmp1, can transform rodent fibroblasts and induce markers of b-lymphocyte activation. the second, lmp2, colocalizes with lmp1 in a constitutive patch in the ebv-transformed b-lymphocyte plasma membrane. the experiments reported here demonstrate that lmp2 may biochemically interact with lmp1 and that lmp2 closely associates with and is an important substrate for a b-lymphoc ... | 1991 | 1710288 |
| identification of two t-cell epitopes on the candidate epstein-barr virus vaccine glycoprotein gp340 recognized by cd4+ t-cell clones. | current efforts to develop an epstein-barr virus subunit vaccine are based on the major envelope glycoprotein gp340. given the central role of cd4+ t cells in regulating immune responses to subunit vaccine antigens, the present study has begun the work of identifying linear epitopes which are recognized by human cd4+ t cells within the 907-amino-acid sequence of gp340. a panel of gp340-specific cd4+ t-cell clones from an epstein-barr virus-immune donor were first assayed for their proliferative ... | 1991 | 1710291 |
| differential expression of epstein barr viral transcripts for two proteins (tp1 and lmp) in lymphocyte and epithelial cells. | studies presented here show that some functions of the human herpesvirus, ebv, may be transcriptionally differentially expressed in two cell types which carry the same (c15) isolate of this virus. of the 'latent' viral functions investigated, only one (tp2) of the episomally-specific genes that encode terminal proteins (tp1 and tp2) is found to be expressed in the c15 epithelial cell tumour environment, whereas both are transcribed--as different, but related, messengers--in a b-cell line generat ... | 1991 | 1710355 |
| allo-cross-reactivity of a human neuraminidase-specific t cell clone dependent on presentation of an endogenous b cell-specific antigen. | t cells specific for foreign antigen recognize a complex of peptides and self-major histocompatibility complex (mhc) molecules and can also cross-react with allo-mhc molecules. it remains controversial, however, what alloreactive t cells exactly recognize. it has been proposed that alloreactive t cells recognize endogenous peptides presented by allo-mhc molecules. to test this hypothesis, we examined an influenza virus-specific t cell clone (6h5), specific for neuraminidase n2 and restricted by ... | 1991 | 1710566 |
| demonstration of epstein-barr virus in scrape material of lateral border of tongue in heart transplant patients by negative staining electron microscopy. | scrape material from the lateral border of the tongue of 50 heart transplant patients and 20 controls was studied for the presence of ebv by negative staining electron microscopy. mild oral hairy leukoplakia was observed in two cases. particles of the herpes virus were found in 20% of the specimens. controls were negative for ebv. the study has shown that ebv may be expressed at the lateral border of the tongue during immunosuppression, occasionally resulting in the clinical appearance of hairy ... | 1991 | 1712389 |
| autoantigen recognition by thyroid-infiltrating t cells in graves disease. | graves disease is a common form of human autoimmune thyroiditis. it shares many pathological features and hla associations with other, less easily studied, organ-specific autoimmune conditions such as insulin-dependent diabetes mellitus, and hence it is also a useful model for understanding these other diseases. we have previously shown that thyroid-infiltrating t cells in graves disease that have been recently activated in vivo specifically recognize autologous thyroid epithelial cells. however ... | 1991 | 1714602 |
| antibody bnh9 detects red blood cell-related antigens on anaplastic large cell (cd30+) lymphomas. | two new monoclonal antibodies--bnh9 and bnf13--were generated by using a human lung adenocarcinoma cell line and standard hybridoma techniques. both were found to react with epithelial and endothelial cells in routinely fixed and embedded tissues. unexpected membrane labelling of some large cell lymphomas while non-reacting with normal lymphoid cells, prompted further characterisation. the antibodies were found to recognise red blood cell-related oligosaccharide antigens. the specificities were ... | 1991 | 1716454 |
| lymphokine secretion and cytotoxic activity of human cd4+ t-cell clones against bordetella pertussis. | human cd4+ t-cell clones specific for pertussis toxin and other bordetella pertussis antigens have been tested for their cytotoxic activity, lymphokine production, and capacity to induce immunoglobulin synthesis. clones specific for the s1 subunit of pertussis toxin were cytotoxic for autologous epstein-barr virus-transformed b cells, which had been pulsed with the native antigen, the recombinant s1 subunit of pertussis toxin, or synthetic peptides derived from the s1 amino acid sequence. the ki ... | 1991 | 1716614 |
| growing human b lymphocytes in the cd40 system. | one approach to generating human monoclonal antibodies relies on the development of culture techniques allowing the long-term growth of most b lymphocytes, a property of the cd40 system. | 1991 | 1717852 |
| nucleotide sequence and transcriptional mapping of the major capsid protein gene of pseudorabies virus. | the gene encoding the 142-kda major capsid protein (mcp142) of pseudorabies virus (prv) was isolated and sequenced. nucleotide sequence analysis revealed that the mcp142 gene has a single open reading frame of 3993 nucleotides (nt) encoding 1330 amino acids. the 4400-nt major rna from the mcp142 gene was detected in prv-infected cells. the 5' end of the transcript was located 60 nt upstream of the initiation codon. the 3' end of the transcript was located 18 nt downstream of a putative poly(a) s ... | 1991 | 1718089 |
| characterization of two monoclonal antibodies to epstein-barr virus diffuse early antigen which react to two different epitopes and have different biological function. | five monoclonal antibodies (mabs) were identified using immunofluorescence that were specific for the epstein-barr virus (ebv) encoded 52/50 kda early antigen (ea-d) protein complex. evidence to suggest that these mabs react with the same 52/50 kda ea-d protein was obtained by western blotting, immunoprecipitation and elisa. two of the mabs, 90e2 and 214a9, neutralized ebv dna polymerase activity. the 214a9 mab also inhibited the activity of bacteriophage t4 dna polymerase while the 90e2 mab did ... | 1991 | 1719014 |
| evidence for defective repair of cyclobutane pyrimidine dimers with normal repair of other dna photoproducts in a transcriptionally active gene transfected into cockayne syndrome cells. | cockayne syndrome (cs) and xeroderma pigmentosum (xp), autosomal recessive diseases with clinical and cellular hypersensitivity to uv radiation, differ in ability to repair uv dna photoproducts in their overall genome: normal repair in cs, defective repair in xp. in order to characterize a dna repair defect in an active gene in cs, we measured the capacity of cells from patients with cs and xp to reactivate 2 major types of uv-induced dna damage, photoreactivatable (i.e., cyclobutane pyrimidine ... | 1991 | 1719400 |
| nonresponsiveness to an immunodominant epstein-barr virus-encoded cytotoxic t-lymphocyte epitope in nuclear antigen 3a: implications for vaccine strategies. | an immunodominant epstein-barr virus (ebv)-encoded cytotoxic t lymphocyte (ctl) epitope has been mapped to the ebv nuclear antigen 3a. the epitope, represented by the peptide sequence awnagflrgraygld (hereafter termed awna), is restricted through the hla-b8 allele and is expressed by type a but not type b-infected transformants. herein, we show that ebv-specific memory ctls from an hla-b8+ healthy virus carrier, js, did not respond in vitro to awna, even though that individual's endogenously inf ... | 1991 | 1719531 |
| expression of epstein-barr virus nuclear antigens in anti-igm-stimulated b cells following recombinant vaccinia infection and their recognition by human cytotoxic t cells. | cytotoxic t lymphocytes (ctl) recognizing epstein-barr virus (ebv) nuclear antigens (ebna) are an important host defence mechanism in restricting the proliferation of ebv-infected b cells. previously, b-type lymphoblastoid cell lines (lcl) infected with vaccinia recombinants encoding for the ebna proteins have been used to identify a-type-specific ctl epitopes. however, to localize the ctl epitopes encoded by both a- and b-type transformants, b-type lcl are an inappropriate host for vaccinia. in ... | 1991 | 1722774 |
| cross reaction of antibodies to a glycine/alanine repeat sequence of epstein-barr virus nuclear antigen-1 with collagen, cytokeratin, and actin. | p62 is a synthetic peptide which corresponds to the glycine/alanine repeat sequence of epstein-barr virus nuclear antigen-1. it is the main epitope recognised by anti-rheumatoid arthritis nuclear antigen antibodies. it was shown previously that anti-p62 antibodies were raised fourfold in patients with rheumatoid arthritis compared with controls. to examine the possibility that this increase was due to cross reactive autoantibodies binding to p62, anti-p62 antibodies from serum samples taken from ... | 1991 | 1722966 |
| [expression of latent membrane proteins (lmp) of epstein-barr virus in malignant lymphomas]. | the presence of epstein-barr virus (ebv) dna and antigens was assessed by polymerase chain reaction and immunohistology, respectively, in a total of 92 cases of hodgkin's disease, angioimmunoblastic lymphadenopathy, cd30-positive anaplastic large cell (alc) lymphomas, and aids-associated atypical lymphoproliferations (alp). proportions of the ebv dna-positive lesions showed latent membrane protein (lmp) expression; some of the lmp-positive alc lymphomas and alp cases also displayed ebna2 immunos ... | 1991 | 1724825 |
| in vitro production of anti-neutrophilocyte-cytoplasm-antibodies (anca) by epstein-barr virus-transformed b-cell lines in wegener's granulomatosis. | the frequent detection of anti-neutrophilocyte-cytoplasm-antibodies (anca) in patients with wegener's granulomatosis (wg) led to the supposition that this disease might be of autoimmune nature. for some authors assume that epstein-barr virus (ebv) infection of human b-lymphocytes besides polyclonal activation could reveal the cryptic immune status against different autoantigens in patients with autoimmune diseases we investigated ebv-transformed b-lymphocytes from patients with sjögren's syndrom ... | 1991 | 1725964 |
| establishment and characterization of a thymic carcinoma cell line (ty-82) carrying t(15;19)(q15;p13) chromosome abnormality. | a new human cell line, designated ty-82, was established from the pleural effusion of a 22-year-old woman with undifferentiated thymic carcinoma. this cell line consisted of primitive cells that were positive for alpha-naphthyl butyrate esterase and acid phosphatase. the cells were shown to express epithelial membrane antigen, but were completely negative for cytokeratin, carcinoembryonic antigen, glial fibrillary acidic protein, desmin, s-100 protein, lysozyme, leu-7, hla-dr (ia), leukocyte com ... | 1992 | 1730520 |
| production of heterohybridomas secreting autoreactive and polyreactive human monoclonal antibodies. | peripheral blood lymphocytes from two polytransfused renal dialysis patients were transformed by epstein-barr virus, fused to a heteromyeloma and cloned. eight human monoclonal antibodies from the resulting clones were tested for their binding to a variety of antigens by elisa, indirect immunofluorescence and immunoblotting. antigens tested included b-cell lines, t and b lymphocytes, red blood cells, chronic lymphocytic leukaemic b cells, igg, ssdna, dsdna, histones, nucleoprotamine, sperm nucle ... | 1992 | 1734494 |
| severe herpes simplex virus hepatitis following autologous bone marrow transplantation: successful treatment with high dose intravenous acyclovir. | a 17-year-old male patient with t-cell type lymphoblastic lymphoma in complete remission underwent high dose chemotherapy (busulfan 16 mg/kg and cyclophosphamide 120 mg/kg) followed by autologous bone marrow transplantation (abmt). the patient had been taking oral acyclovir (200 mg x 5) daily from seven days prior to the abmt (day -7). on day +24, he complained of epigastralgia and general malaise, and the next day his got and gpt rose to 570 u/l and 397 u/l, respectively. although he had no muc ... | 1991 | 1753418 |
| intrathoracic carcinoma in an 11-year-old girl showing a translocation t(15;19). | we report here a rare case of intrathoracic undifferentiated carcinoma in an 11-year-old girl. the biopsy tissue stained with antibodies against cytokeratin and carcino-embryonic antigen. the same staining was obtained using a cell line, per-403, which was derived from the tumor. dna from per-403 cells was tested for the presence of epstein-barr virus genes, yielding a negative result. the cytogenetic analysis found a translocation t(15;19) (p12;q13), which has not previously been described in a ... | 1991 | 1785673 |
| a rabies-specific human monoclonal antibody that protects mice against lethal rabies. | according to a recommendation from who (world health organisation) for prevention of a possible rabies infection, active vaccination has to be combined with application of immunoglobulin to get a fast protective effect. at present, preparations of purified human or equine rabies-specific immunoglobulin are used. we have generated a human rabies-specific monoclonal antibody (humab) by immortalization of human b-cells with epstein barr virus (ebv), followed by fusion with a mouse myeloma cell. the ... | 1991 | 1804770 |
| paucity of humoral response in patients to glioma-associated antigen(s): antigen localization by immunofluorescence. | xenogeneic immunization of freshly-prepared human glioma extracts into goats has yielded a polyclonal antiserum, which after multiple absorptions specifically identifies antigenic entities only in glioma extracts, and not in appropriate controls, both by radioimmunoassays (rias) and western immunoblots. the results from the absorbed polyclonal antiserum have been confirmed by the successful generation of six stable murine monoclonal antibodies (mabs) which recognize a subset of the same antigens ... | 1991 | 1805570 |
| polyreactivity of human monoclonal antibodies: human anti-rh monoclonal antibodies of igm isotype are frequently polyreactive. | the specific aim of this study was to characterize human anti-rh monoclonal antibodies cross-reacting with self-antigens. we studied supernatants from man-mouse hybridomas and from lymphoblastoid cell lines. man-mouse hybridomas were established by fusion of peripheral blood lymphocytes from healthy individuals recently immunized against rh alloantigens, with mouse myeloma (or man-mouse heteromyeloma) cell lines. lymphoblastoid cell lines were produced by epstein-barr virus induction of lymphocy ... | 1991 | 1807061 |
| expression of a human monoclonal anti-(rhesus d) fab fragment in escherichia coli with the use of bacteriophage lambda vectors. | a human anti-(rhesus d) antibody (igg1 lambda) fab fragment was cloned from an epstein-barr-virus-transformed cell line and expressed in escherichia coli with the use of bacteriophage lambda vectors. the cloned protein is active in binding to human erythrocytes and permits the development of a recombinant reagent for the prevention of haemolytic disease of the newborn. the method offers a rapid and effective means of rescuing human fabs from potentially unstable cell lines secreting human antibo ... | 1991 | 1830475 |
| human b cells cannot be triggered to kill target cells through their fc gamma rii or fc epsilon rii receptors. | there has been some controversy as to whether or not b cells can kill target cells through their fc receptors. to address this, we have examined the ability of human b cells from a variety of sources to lyse hybridoma cells with specificity for either the b cell fc gamma rii or fc epsilon rii using a reverse killing assay, as well as their ability to lyse opsonized chicken erythrocytes using a classic adcc assay. tonsil b cells, chronic lymphocytic leukemia b cells, and epstein-barr virus-induce ... | 1991 | 1832141 |
| antibody to human t-lymphotropic virus in a patient with guillain-barré syndrome (case report) | serum sample obtained from a male, 12 year old patient suffering from guillain-barré syndrome (gbs) was positive for human t-lymphotropic virus (htlv-i) antibody by the enzyme-linked immunosorbent assay (elisa) and the western blot analysis (wb). attempts to isolate enteroviruses (including poliovirus) from faecal material in both tissue culture and suckling mice were unsuccessful; in addition, acute and convalescent paired serum samples did not show any evidence of recent poliovirus infection w ... | 1991 | 1844957 |
| regulated expression of the rb "tumor suppressor gene" in normal lymphocyte mitogenesis: elevated expression in transformed leukocytes and role as a "status quo" gene. | expression of the rb retinoblastoma tumor suppressor gene product is regulated early during the stimulation of normal human peripheral blood lymphocytes, suggesting a regulatory role for the amount of this protein in mitogenesis of normal cells. when normal human peripheral blood lymphocytes were mitogenically stimulated with pokeweed mitogen, bivariate flow cytometric measurements of cellular dna and rb protein content showed an early decrease in the amount of rb protein per cell, anteceding on ... | 1991 | 1845796 |
| detection of anti-epstein-barr virus trans-activator (zebra) antibodies in sera from patients with human immunodeficiency virus. | patients with aids and aids-related complex often show symptoms of epstein-barr virus (ebv) reactivation. several ebv-encoded trans-acting factors activate the ebv lytic cycle, and one, zebra (bamhi z ebv replication activator; also called eb1), switches ebv from its latent to productive cycle. indirect immunofluorescence studies were done using human cells transfected with a recombinant dna-harboring cdna sequence spanning bzlf1 (bamhi z left frame 1) that was inserted downstream of the adenovi ... | 1991 | 1845809 |
| chronic fatigue syndrome. | 1991 | 1845816 | |
| identification of a novel ebv-induced membrane glycoprotein of 43 kda with h667 mab. | using purified b95-8 epstein-barr virus (ebv), a mab designated h667 was produced. we demonstrated by indirect membrane immunofluorescence (if) on six ebv producer cell lines and by immunoelectron microscopy that h667 reacted with a membrane antigen. h667 recognized a 43-kda ebv protein (p43) as determined by immunoblotting using purified ebv from the six producer cell lines. phosphonoacetic acid treatment of b95-8 cells was associated with the disappearance of p43, indicating that it was a late ... | 1991 | 1845828 |
| epstein-barr virus (ebv)-associated lymphoproliferative disease in the scid mouse model: implications for the pathogenesis of ebv-positive lymphomas in man. | when human peripheral blood lymphocytes (pbls) from epstein-barr virus (ebv)-seropositive donors are injected intraperitoneally into scid mice, ebv+ b cell tumors develop within weeks. a preliminary report (mosier, d. e., r. j. gulizia, s. m. baird, d. d. richman, d. b. wilson, r. i. fox, and t. j. kipps, 1989. blood. 74(suppl. 1):52a) has suggested that such tumors resemble the ebv-positive malignancy, burkitt's lymphoma. the present work shows that generally the human (hu) pbl-scid tumors are ... | 1991 | 1845872 |
| single-stranded structures are present within plasmids containing the epstein-barr virus latent origin of replication. | the epstein-barr virus (ebv) latent origin of plasmid replication (orip) contains two essential regions, a family of repeats with 20 imperfect copies of a 30-bp sequence and a dyad symmetry element with four similar 30-bp repeats. each of the repeats has an internal palindromic sequence and can bind ebna 1, a protein that together with orip constitutes the only viral element necessary for ebv maintenance and replication. using single-strand-specific nucleases, we have probed plasmids containing ... | 1991 | 1846191 |
| epstein-barr virus (ebv) glycoprotein gp350 expressed on transfected cells resistant to natural killer cell activity serves as a target antigen for ebv-specific antibody-dependent cellular cytotoxicity. | cell surface-associated viral glycoproteins are thought to play a major role as target antigens in cellular cytotoxicity and antiviral immunosurveillance. one such glycoprotein is the epstein-barr virus (ebv)-encoded glycoprotein 350 (gp350), which is expressed on both virion envelope and ebv producer cells and carries the virus attachment protein moiety. although it is known that some antibodies to gp350 can neutralize the virus, the role of this glycoprotein in ebv-specific cellular cytotoxici ... | 1991 | 1846213 |
| inhibition of 12-o-tetradecanoylphorbol-13-acetate-promoted mouse skin papilloma by saponins. | papillomas in mouse skin were initiated with 390 nmol of 7,12-dimethylbenz(a)anthracene and 1 week later, were promoted 2 times a week with 4.1 nmol of 12-o-tetradecanoylphorbol-13-acetate (tpa). five saponin-related compounds used as potential anti-inflammatory agents were applied in the same position with 82 nmol. saponin-related compounds effectively inhibited tumor formation in the sensitive mouse stock even when these compounds were given 1 h prior to tpa treatment. these results suggested ... | 1991 | 1846234 |
| detection of human papillomavirus dna in anal intraepithelial neoplasia and anal cancer. | forty anal paraffin-embedded tissue specimens from 24 subjects were studied for the presence of human papillomavirus (hpv) types 6, 11, 16, 18, 31, and 33, herpes simplex virus (hsv), epstein-barr virus, and cytomegalovirus dna by using the polymerase chain reaction. these tissues ranged from histologically normal to invasive squamous cell carcinoma. hpv dna was detected in the invasive anal cancer tissues of 11 of 13 subjects. hpv types were segregated by histopathological severity, with hpv 16 ... | 1991 | 1846314 |
| expression of epstein-barr virus-encoded growth-transformation-associated proteins in lymphoproliferations of bone-marrow transplant recipients. | the expression of epstein-barr virus (ebv)-encoded, growth-transformation-associated proteins was studied in lymphoproliferations of 9 allogeneic bone-marrow transplant (bmt) recipients. immunoblots of cell lysates were probed with polyspecific and monospecific antisera directed against ebna 1, 2, 3 and 6, and the membrane protein lmp. all tumors expressed ebna 1 and lmp. ebna 2 was detected in the tumors of 8 patients, and ebna 3 and 6 in the tumors of 5 patients. the lmp regulatory sequences, ... | 1991 | 1846349 |
| opportunistic viral hepatitis in liver transplant recipients. | 1991 | 1846476 | |
| ig repertoire of human polyspecific antibodies and b cell ontogeny. | a total of 463 ebv ig-secreting clones were derived from embryonic tissues, cord blood, and adult peripheral blood. subcloning and analysis of the h and k loci (germline vs rearranged dna status) of 44 primary clones insured clonality in at least 92% of cases. whatever the cell origin, a somewhat constant proportion of clones (i.e., 11 to 16%) expressed polyspecific antibodies when tested on a panel of nine ag, including self-ag. the vh and vk repertoires have been studied using vh1-vh6 and vk1- ... | 1991 | 1846900 |
| detection of herpesvirus dna in vitreous and aqueous specimens by the polymerase chain reaction. | members of the herpesvirus family, cytomegalovirus (cmv), epstein-barr virus (ebv), and herpes simplex virus (hsv), have been recognized as causal agents of chorioretinal inflammatory diseases. we investigated the use of the polymerase chain reaction for the detection of cmv, hsv, and ebv genomes in aqueous, subretinal fluid, and vitreous specimens in patients with clinically diagnosed cmv retinitis. cytomegalovirus but not hsv or ebv genomic sequences were detected in all of these clinical spec ... | 1991 | 1847043 |
| aggressive peripheral t-cell lymphomas containing epstein-barr viral dna: a clinicopathologic and molecular analysis. | the epstein-barr virus (ebv) has been shown to be associated with posttransplant lymphoma, hodgkin's disease, and t-cell lymphoma, in addition to african burkitt's lymphoma. in a retrospective study of 56 consecutive cases of t-cell lymphoma, ebv dna was found by southern blot and in situ dna hybridization in 10 (20%) of 50 peripheral t-cell lymphomas, but in none of six cases of t-lymphoblastic lymphoma. peripheral t-cell lymphomas containing ebv dna could be subclassified into three categories ... | 1991 | 1847084 |
| platelet-activating factor induces phospholipid turnover, calcium flux, arachidonic acid liberation, eicosanoid generation, and oncogene expression in a human b cell line. | platelet-activating factor is a potent mediator of the inflammatory response. studies of the actions of platelet-activating factor have centered mainly around neutrophils, monocytes, and platelets. in this report we begin to uncover the influence of platelet-activating factor on b lymphocytes. employing the ebv-transformed human b cell line skw6.4, we demonstrate that platelet-activating factor significantly alters membrane phospholipid metabolism indicated by the incorporation of 32p into phosp ... | 1991 | 1847170 |
| epstein-barr virus infection precedes clonal expansion in burkitt's and acquired immunodeficiency syndrome-associated lymphoma. | the epstein-barr virus (ebv) is associated with distinct forms of human lymphoid malignancies, including the endemic (ebl) and sporadic forms of burkitt's lymphoma (sbl) and acquired immunodeficiency syndrome-associated non-hodgkin lymphoma (aids-nhl). however, whether ebv has a pathogenetic role in these tumors or is a passenger virus has not been conclusively demonstrated. one element to distinguish between these two possibilities is to determine whether ebv infection has preceded and, thus, p ... | 1991 | 1847310 |
| variable expression of latent membrane protein in nasopharyngeal carcinoma can be related to methylation status of the epstein-barr virus bnlf-1 5'-flanking region. | seven virus-coded proteins, the nuclear proteins ebna-1 to ebna-6 and the latent membrane protein (lmp), are regularly expressed in epstein-barr virus (ebv)-transformed lymphoblastoid cell lines. in nasopharyngeal carcinoma (npc), only ebna-1 is regularly expressed; lmp is detected in about 65% of the tumors. in burkitt's lymphoma tumors only ebna-1 is expressed. we have recently shown that the methylation patterns of the ebv genome varied between these cell types. in virally transformed lymphob ... | 1991 | 1847471 |
| characterization of the epstein-barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen blast-1 (cd48). | blast-1 (cd48) (previously referred to as bcm-1 by the human gene nomenclature committee) is an early-activation-associated membrane glycoprotein expressed on the surface of human leukocytes and induced to a high level following infection of b cells by the epstein-barr virus. it is a member of the immunoglobulin superfamily, mediates cell adhesion, and has significant sequence homology to two other adhesion molecules, cd2 and lfa3. here we report the isolation and characterization of the blast-1 ... | 1991 | 1847502 |
| establishment of a lymphoblastoid cell line, sd-1, expressing the p190 bcr-abl chimaeric protein. | a lymphoblastoid cell line (sd-1) has been established by epstein-barr virus immortalisation of philadelphia chromosome positive acute lymphoblastic leukaemia (all) cells. using newly derived anti-bcr monoclonal and anti-abl polyclonal antibodies it was demonstrated that both the original leukaemic cells and the derived cell line expressed the p190 form of the bcr-abl protein found in a proportion of cases of philadelphia chromosome positive all. interestingly, the leukaemia and the derived cell ... | 1991 | 1847983 |
| the bzlf1 protein of ebv has a coiled coil dimerisation domain without a heptad leucine repeat but with homology to the c/ebp leucine zipper. | the ebv transactivator protein bzlf1 can bind many sites in the ebv genome, most of which have homology to a consensus ap-1 site, the binding site for the fos/jun family of transcription factors. here we present evidence that bzlf1 can also recognise the binding site for the ccaat/enhancer binding protein c/ebp and that a bzlf1 binding site within the bzlf1 promoter is recognised by the c/ebp protein. analysis of the bzlf1 dna binding domain suggests that the bzlf1 protein binds to dna as a dime ... | 1991 | 1847997 |
| demonstration of epstein-barr virus in primary central nervous system lymphomas. | 1991 | 1848206 | |
| detection of epstein-barr virus dna by in situ hybridization and polymerase chain reaction in salivary gland biopsy specimens from patients with sjögren's syndrome. | to determine whether epstein-barr virus (ebv) could be involved in the pathogenesis of sjögren's syndrome (ss). | 1991 | 1848394 |
| soluble interleukin-2 receptors in patients with nasopharyngeal carcinoma. | the authors performed a retrospective analysis of serum soluble interleukin-2 receptor (sil-2r) levels in 72 patients with nasopharyngeal carcinoma (npc) using an enzyme immunoassay. their objectives were to determine the value of serum sil-2r in estimating the tumor burden, and its predictive value in response to therapy and prognosis. the data showed that serum sil-2r levels in patients were significantly higher than that of healthy controls. the serum levels correlated with clinical staging a ... | 1991 | 1848475 |
| epstein-barr virus lymphoproliferative disease associated with acquired immunodeficiency. | epstein-barr virus (ebv) lymphoproliferative disease is seen in patients with both congenital and acquired immunodeficiencies. lymphoproliferative disease has been reported in 1 to 3% of renal transplant recipients. most patients presented with solid tumor masses, rather than an infectious mononucleosis-like syndrome. about one third of cases had involvement of the renal allograft with tumor; the small intestine or central nervous system was also frequently affected. about half of the patients s ... | 1991 | 1848644 |
| murine erythroleukemia cell line gm979 contains factors that can activate silent chromosomal human gamma-globin genes. | we introduced a normal chromosome 11 into gm979 murine erythroleukemia cells by fusing them with epstein-barr virus-transformed lymphocytes from a normal individual. in contrast to previous data obtained with other murine erythroleukemia cells, we detected activation of human chromosomal gamma-globin genes in gm979 cells. gm979, unlike previously used murine erythroleukemia cell lines, expresses murine embryonic globin in addition to adult globin. while all the hybrids expressed gamma- and beta- ... | 1991 | 1848707 |
| elevated creatine kinase levels. | 1991 | 1848909 | |
| amino acid sequence homology of epstein-barr virus nuclear antigen-2 to adenovirus e1a, human papilloma virus-16 e7 and sv40 large t antigen in the functional domains for growth transformation. | 1991 | 1849247 | |
| detection of primary epstein-barr virus infection in a patient with x-linked lymphoproliferative disease receiving immunoglobulin prophylaxis. | serologic diagnosis for epstein-barr virus (ebv) infection is problematic when patients receive exogenous immunoglobulin. we recently diagnosed primary ebv infection by detecting ebv-determined nuclear antigen (ebna) and ebv-dna in peripheral blood mononuclear cells (pbmc) using immunofluorescence, in situ hybridization and polymerase chain reaction (pcr) techniques in a patient with x-linked lymphoproliferative disease (xlp) who received prophylactic immunoglobulins for ebv infection. these com ... | 1991 | 1849350 |
| a new non-hodgkin's b-cell line (dohh2) with a chromosomal translocation t(14;18)(q32;q21). | a spontaneously growing ebv-negative b-cell line (dohh2) was established from the pleural fluid cells of a 60-year-old man with centroblastic/centrocytic non-hodgkin's lymphoma, that had transformed into an immunoblastic lymphoma. the pleural fluid cells and the dohh2 cells expressed igg lambda, were reactive with cd10 and cd19 monoclonal antibodies, and showed by cytogenetic analysis 48,xy, +7, +del(12)(q24), t(14;18)(q32;q21). southern blot analysis of mini-satellite dna patterns, and of rearr ... | 1991 | 1849602 |
| early events in epstein-barr virus infection of human b lymphocytes. | the sequence of epstein-barr virus (ebv) and b lymphocyte changes in the 3 days following acute infection was analyzed. by 16 hr the average infected lymphocyte had 1 ebv episome. nuclear protein-2 (ebna-2) and ebna-leader protein (-lp) were detected by 12 hr, and by 32 hr were at the levels of stable ebv infection in lymphoblastoid cell lines (lcls). at 12 hr, all ebna-lp and ebna-2 rnas were initiated from the pw promoter. by 36 hr a significant ebna-lp and ebna-2 rna fraction initiated from t ... | 1991 | 1849678 |
| cellular origins of serum complement receptor type 2 in normal individuals and in hypogammaglobulinaemia. | a soluble form of complement receptor 2 (scr2) is found in normal human serum. amounts present are about 30-90 ng/ml, which is of the same order as reported for soluble cr1. although b cells express surface cr2 and are the main peripheral blood source of scr2 they do not appear to be the major tissue source of serum scr2. serum levels of scr2 of patients with hypogammaglobulinaemia were not significantly different from those of normal individuals even in the case of two brothers with bruton's x- ... | 1991 | 1849806 |
| high recurrence of rearrangements involving chromosome 14 in an ataxia telangiectasia lymphoblastoid cell line and in its mutagen-treated derivatives. | the cytogenetic characterization of ch cell line obtained by epstein-barr-virus transformation of the lymphocytes of a patient affected by ataxia telangiectasia is reported. control ch cells and 2 subcultures treated with the mutagens r7000 or nqo were developed in parallel and studied. a common chromosome anomaly, a der(14) t(11;14) (q13.2;q32), was found in all the studied karyotypes, indicating that it occurred either in vivo or early in vitro. in non-treated cultures, additional anomalies we ... | 1991 | 1849869 |
| epstein-barr virus bhrf1 gene but not the cellular protooncogene bcl-2 is expressed in ataxia-telangiectasia lymphoblastoid lines. | the expression of both the epstein-barr virus (ebv) orf bhrf1 and the cellular protooncogene bcl-2 was studied in ebv-transformed lymphoblastoid b cells from patients with the human genetic disorder ataxia-telangiectasia (a-t). using the northern blot technique, it was found that the pattern of transcription of the bhrf1 gene in a-t lymphoblastoids resembled that in ebv-transformed normal lymphoblastoid lines and burkitt lymphoma (bl) lymphocytes. however, the 1.5-kb mature bhrf1 mrna species pr ... | 1991 | 1850186 |
| an epstein-barr virus-negative burkitt lymphoma cell line (sframos) secretes a prolactin-like protein during continuous growth in serum-free medium. | pituitary human prl (hprl) antiserum inhibits growth of b-lymphoblastoid cells in vitro, but the mechanism of inhibition is unclear. in this study the mechanism of inhibition of human b-cell growth by anti-hprl was explored with an epstein-barr virus nuclear antigen (ebna)-negative burkitt lymphoma cell line (sframos) that proliferates continuously in serum-free medium with human transferrin as the only protein supplement. the data show that antiserum immunoglobulin fraction g (igg) to pituitary ... | 1991 | 1850347 |
| orthotopic liver transplantation, epstein-barr virus, cyclosporine, and lymphoproliferative disease: a growing concern. | lymphoproliferative disease (lpd) is a well-recognized complication of both solid organ and bone marrow transplantations. the occurrence of lpd in these settings is related in part to the use of the immunosuppressive agent cyclosporine. we report 12 cases of lpd after orthotopic liver transplantations in 132 pediatric patients. lymphoproliferative disease occurred as one of three clinical syndromes: (1) lymphadenopathic, (2) systemic, and (3) lymphomatous. effective management of lpd with excisi ... | 1991 | 1850458 |
| detection of epstein-barr virus with molecular hybridization techniques. | the cord-blood transformation assay remains the standard method for detecting epstein-barr virus (ebv) in secretions. however, newer methods are much faster and more sensitive, although most are still regarded as research procedures. the most useful of these are southern blot hybridization, particularly the variation that employs terminal genomic probe analysis; in situ cytohybridization; and polymerase chain reaction analyses. use of these methods alone or in combination should disclose the inf ... | 1991 | 1850538 |
| serologic and immunologic responses in chronic fatigue syndrome with emphasis on the epstein-barr virus. | although patients with chronic fatigue syndrome (cfs) can be diagnosed by clinical criteria, the lack of specific laboratory criteria delays or prevents the diagnosis and contributes to the quasi-disease status of the syndrome. a resurgence of interest in the syndrome has followed reports suggesting that cfs may be associated with chronic active infection due to the epstein-barr virus. analysis of reports to date shows that the mean titers of antibodies to viral capsid antigen and to early antig ... | 1991 | 1850541 |
| chronic fatigue syndrome in northern nevada. | the clinical and laboratory findings from studies of patients with chronic fatigue syndrome (cfs) from northern nevada are summarized. physicians caring for these patients have estimated that greater than 400 patients with cfs from northern nevada and nearby communities in california were identified between 1984 and 1988. as a result of these studies, a cluster of clinical and laboratory features associated with the illness in moderately to severely affected patients has been identified: profoun ... | 1991 | 1850542 |
| characterization of 3'-to 5'-exonuclease activity associated with epstein-barr virus dna polymerase. | epstein-barr virus (ebv) dna polymerase mediates viral dna replication during the lytic phase of the eb virus life cycle. in order to characterize its enzymatic activities ebv dna polymerase was purified more than 1200-fold from chemically induced b95-8 cells. one polypeptide with molecular weight of 110,000 corresponded to the predicted ebv dna polymerase, whereas the other polypeptides did not. a 3'-to-5' exonuclease activity was copurified with the ebv dna polymerase through the course of the ... | 1991 | 1850910 |
| natural killer-mediated lysis of some but not all hsv-1- or vsv-infected targets requires the participation of hla-dr-positive accessory cells. | natural killer (nk)-mediated lysis of herpes simplex virus type 1-infected fibroblasts (hsv-fs) has been previously shown to require the co-operation of cd16-positive nk cells and an hla-dr-positive accessory cell population. in contrast, lysis of k562 tumour cells requires the presence of only the leu-11-positive cells. in the current study, targets of different morphologies, both virally infected and non-infected, were tested in an attempt to dissect out which target characteristics determine ... | 1991 | 1851136 |
| autocrine regulation of normal and malignant b lymphocytes. | 1991 | 1851375 | |
| clustering of residence of multiple sclerosis patients at age 13 to 20 years in hordaland, norway. | geographic and temporal variation and migration studies point to an exogenous agent in the etiology of multiple sclerosis. if infectious etiology is involved, space-time clustering would also be expected. the authors analyzed 381 patients with a clinical onset of multiple sclerosis between 1953 and 1987 in the county of hordaland, norway. patients within the same birth cohort had lived significantly closer to each other than would be expected during ages 13-20 years, with peak clustering at age ... | 1991 | 1851394 |
| transcriptional interference between the ebv transcription factors eb1 and r: both dna-binding and activation domains of eb1 are required. | the switch from latency to a productive infection in ebv-infected b cells is linked to the expression of two viral sequence-specific dna-binding transcription factors called eb1 and r. eb1 shares sequence homologies with the bzip family of proteins in the basic region required for specific dna interaction. here, we provide evidence that eb1 and r can synergistically activate specific transcription, and that overexpressed, unbound eb1, represses the r-induced transcription ('squelching'). in orde ... | 1991 | 1851554 |
| establishment and characterization of an epstein-barr virus spontaneously transformed lymphocytic cell line derived from a hairy cell leukemia patient. | hairy cell leukemia is a rare, b-cell malignancy uniquely sensitive to the antitumor effects of alpha and beta interferons (ifn). in order to further study the effects of ifn in this disease, we derived a cell line (hc1) from the peripheral blood mononuclear cells of a patient with hairy cell leukemia (hcl). cells exhibited the typical morphological features of hcl, including the characteristic cytoplasmic projections by light, transmission, and scanning electron microscopy. hc1 cells were of b- ... | 1991 | 1851909 |
| chronic fatigue in adolescents. | nine female and 6 male adolescents (mean age 14.5 +/- 1.7 [sd] years) were evaluated for chronic fatigue associated with at least three additional symptoms present for 18.4 +/- 8.4 months. eleven subjects experienced the onset of symptoms with an acute illness (seven monospot-positive). medical history, physical examination, and laboratory testing yielded little helpful information. serologic testing for coxsackie b viruses 1 through 6, cytomegalovirus, epstein-barr virus, human herpesvirus 6, a ... | 1991 | 1861915 |
| herpes zoster and controlateral hemiplegia in an african patient infected with hiv-1. | one of the neurologic complications of human immunodeficiency virus infection are cerebrovascular accidents. in hiv infected patients, ischemic strokes have been reported secondary to nonbacterial thrombotic endocarditis and cerebral arteritis. we describe an unusual cause of stroke in hiv-1 infection: herpes zoster ophtalmicus with contralateral hemiplegia. | 1991 | 1867123 |
| common expression of melanoma tumor-associated antigens recognized by human tumor infiltrating lymphocytes: analysis by human lymphocyte antigen restriction. | major histocompatibility complex (mhc) class i antigens (ag), particularly human lymphocyte antigen (hla)-a2, have been shown to function as restriction elements in human cytotoxic t lymphocyte recognition of tumor. this study was undertaken to determine the function of non-a2 mhc class i ag in tumor recognition by tumor-infiltrating lymphocytes (tils) cultured from six melanomas, and to find evidence for shared or unique tumor-associated ag. four predominantly cd8+ and two mixed cd4+, cd8+ popu ... | 1991 | 1868040 |
| marked cell-type-specific differences in glycosylation of human interleukin-6. | human interleukin-6 (il-6) secreted by cytokine- or endotoxin-induced fibroblasts, monocytes, keratinocytes, endometrial stromal cells, and endothelial cells, when analyzed under denaturing and reducing conditions, consists of a set of differentially modified phosphoglycoproteins of molecular mass in the range from 23 to 30 kd (a set of at least three o-glycosylated 23- to 25-kd species and a set of at least three n- and o-glycosylated 28- to 30-kd species). the 23- to 25-kd and 28- to 30-kd fib ... | 1991 | 1883960 |
| synthesis, isolation, and characterization of endogenous beta-galactoside-binding lectins in human leukocytes. | galaptin, a beta-galactoside-binding lectin, was isolated from human buffy coat cells (peripheral leukocytes) and spleen by affinity chromatography. the molecular weight (32k) of the native buffy coat galaptin was similar to that for splenic galaptin. their subunit molecular weight (14.5k), pi (4.60-4.85), and amino acid composition were identical. both galaptins showed the presence of a single polypeptide when subjected to reversed-phase hplc. monospecific rabbit polyclonal antiserum raised aga ... | 1991 | 1888747 |
| postinfectious chronic fatigue syndrome: case history of thirty-five patients in germany. | thirty-five patients with chronic fatigue syndrome according to the criteria of holmes were followed for periods of up to eight years. the most frequent symptoms were severe fatigue, arthralgias and myalgias, recurrent oropharyngitis and various psychiatric disorders. more than half of the patients suffered from neuropathy, lymphadenopathy, gastrointestinal complaints and recurrent low-grade fever. recurrent or persistent activity of human herpesvirus -6 infection was seen in 73% of the patients ... | 1991 | 1893076 |
| tissue distribution of mucosal antibody-producing cells specific for respiratory syncytial virus in severe combined immune deficiency (scid) mice engrafted with human tonsils. | groups of c.b-17 scid mice were reconstituted intraperitoneally with human tonsillar mononuclear cells (hu-tmc) from children seropositive for antibody to respiratory syncytial virus (rsv) and subsequently challenged intraperitoneally with inactivated rsv or sham-immunized. the synthesis and the distribution characteristics of human antibody to rsv in various murine tissues were studied using an enzyme-linked immunospot assay (elispot). no specific antibody was observed in sham-immunized animals ... | 1991 | 1893614 |
| cellular origins of human polymeric and monomeric iga: enumeration of single cells secreting polymeric iga1 and iga2 in peripheral blood, bone marrow, spleen, gingiva and synovial tissue. | using modified elisa and spot-elisa, which permit the parallel determination of heavy chain subclass and the presence of covalently linked j chain, we analysed iga found in cell culture supernatants or secreted by individual cells from peripheral blood, spleen, bone marrow, gingiva and synovial tissue, with respect to its polymeric or monomeric iga form (piga, miga) and iga1 or iga2 subclass. the elisa for determination of j chain in tissue culture supernatants was specific and highly sensitive ... | 1991 | 1907532 |
| molecular definition of a polymorphic antigen (la45) of free hla-a and -b heavy chains found on the surfaces of activated b and t cells. | a monomoprhic monoclonal antibody (la45 antibody) reactive with "a new activation-induced surface structure on human t lymphocytes" (la45 antigen) that resembled free class i heavy chains has recently been described (schnabl, e., h. stockinger, o. majdic, h. gaugitsch, i.j.d. lindley, d. maurer, a. hajek-rosenmayr, and w. knapp. 1990. j. exp. med. 171:1431). this antibody was used to clone a class i-like heavy chain (la45 gene) from the hut 102 tumor cell, which paradoxically did not give rise t ... | 1991 | 1940790 |
| topological effects of ebna 1 on orip. | 1990 | 1963028 | |
| epstein-barr virus, burkitt's lymphoma, and an african tumor promoter. | 1990 | 1963031 | |
| epstein-barr virus terminal protein gene transcription is dependent on ebna2 expression and provides evidence for viral integration into the host genome. | 1990 | 1963585 | |
| effect of tgf-beta on the proliferation of b cell lines and on the immortalisation of b cells by ebv. | 1990 | 1963587 | |
| b-cell lymphoma in organ transplant recipients. | 1990 | 1964395 | |
| a chronic "postinfectious" fatigue syndrome associated with benign lymphoproliferation, b-cell proliferation, and active replication of human herpesvirus-6. | a 17-year-old, previously healthy woman developed an acute "mononucleosis-like" illness with an associated "atypical" pneumonitis, followed by years of debilitating chronic fatigue, fevers, a 10-kg weight loss, night sweats, and neurocognitive symptoms. thereafter, her sister developed a similar but less severe illness. the patient developed marked, chronic lymphadenopathy and splenomegaly, with associated persistent relative lymphocytosis and atypical lymphocytosis and with thrombocytopenia. af ... | 1990 | 1964694 |
| amplification of dna sequences of epstein-barr and human immunodeficiency viruses using dna-polymerase from thermus thermophilus. | using thermophilic dna-polymerase from thermus thermophilus we have amplified by polymerase chain reaction (pcr) specific dna sequences of epstein-barr virus (ebv) and human immunodeficiency virus (hiv). dna-polymerase from thermus thermophilus (molecular mass of 80-86 kda) differs in its physico-chemical properties from dna-polymerase from thermus aquaticus (molecular mass of 62-68 kda). to amplify the specific ebv dna sequence, oligonucleotide primers for the virus replicon region (orip region ... | 1990 | 1965009 |
| [ebv replication and therapy of oral hairy leukoplakia using acyclovir]. | the therapy of choice for oral hairy leucoplakia in hiv-infected patients is treatment with acyclovir. during treatment the replication of epstein-barr virus (ebv) in cells scraped from the epithelium of hairy leucoplakia was investigated using filter-in-situ hybridization. on the 2nd day of treatment a slight and on the 5th day a marked reduction of the replication was observed, and on the 8th day of treatment replication of ebv could hardly be detected. at that time a marked regression of leuc ... | 1990 | 1965185 |
| recent experimental studies in lymphoma: molecular and virologic aspects. | 1990 | 1965693 | |
| acquired immunodeficiency syndrome and epstein-barr virus. | epstein-barr virus is an important aetiological factor in certain hiv-related syndromes, with its opportunist expression related to the level of host immunodeficiency. in asymptomatic people co-infected with hiv, ebv activity is reflected by increased viral shedding and rises in anti-ebv titres; as immunodeficiency ensues ebv manifests as epithelial hyperproliferation in ohl, and later as b-cell lymphoma in aids. the suggested role of ebv as a co-factor in the progression of hiv infection and de ... | 1990 | 1965885 |
| syndromes of inappropriate macrophage activation in childhood. | the concept of inappropriate macrophage activation (ima) embraces the clinicopathologic conditions associated with non-malignant lymphohistiocytic proliferation: familial lymphohistiocytosis (flh) and hemophagocytic syndromes associated with viral, bacterial, fungal or parasitic infections in both immunodeficient and immunocompetent patients. ima is also observed during the clinical course of primary immunodeficiencies (chediak higashi). the mechanisms of ima are still unknown. prognosis varies ... | 1990 | 1966290 |