Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [salmonella multiphasic flagellar antigen]. | in the salmonella antigenic pattern, more than one phase of flagellar antigen is observed. the phase of flagellar antigen depends of the gene which encodes the protein building the filament of flagella. the flic gene encodes the 1st phase of flagellar antigen and the fljb gene encodes the 2nd phase of flagellar antigen. the third phase of flagellar antigen is encoded by one of the genes localized on the plasmid. expression of the fljb gene (part of the hinfljba operon) is regulated by a mechanis ... | 2012 | 22922144 |
| type i interferon induces necroptosis in macrophages during infection with salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium (s. typhimurium) is a virulent pathogen that induces rapid host death. here we observed that host survival after infection with s. typhimurium was enhanced in the absence of type i interferon signaling, with improved survival of mice deficient in the receptor for type i interferons (ifnar1(-/-) mice) that was attributed to macrophages. although there was no impairment in cytokine expression or inflammasome activation in ifnar1(-/-) macrophages, they were h ... | 2012 | 22922364 |
| review of pathogenesis and diagnostic methods of immediate relevance for epidemiology and control of salmonella dublin in cattle. | salmonella enterica subsp. enterica serovar dublin (s. dublin) receives increasing attention in cattle production. it is host-adapted to cattle, and leads to unacceptable levels of morbidity, mortality and production losses in both newly and persistently infected herds. cattle health promoting institutions in several countries are currently constructing active surveillance programmes or voluntary certification programmes, and encourage control and eradication of s. dublin infected cattle herds. ... | 2013 | 22925272 |
| cathelicidin antimicrobial peptide expression is not induced or required for bacterial clearance during salmonella enterica infection of human monocyte-derived macrophages. | salmonella enterica serovar typhimurium is able to resist antimicrobial peptide killing by induction of the phop-phoq and pmra-pmrb two-component systems and the lipopolysaccharide (lps) modifications they mediate. murine cathelin-related antimicrobial peptide (cramp) has been reported to inhibit s. typhimurium growth in vitro and in vivo. we hypothesize that infection of human monocyte-derived macrophages (mdms) with salmonella enterica serovar typhi and s. typhimurium will induce human catheli ... | 2012 | 22927052 |
| interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments. | bacterial microcompartments (mcps) are a widespread family of proteinaceous organelles that consist of metabolic enzymes encapsulated within a protein shell. for mcps to function specific enzymes must be encapsulated. we recently reported that a short n-terminal targeting sequence of propionaldehyde dehydrogenase (pdup) is necessary and sufficient for the packaging of enzymes into a mcp that functions in 1,2-propanediol (1,2-pd) utilization (pdu) by salmonella enterica. here we show that encapsu ... | 2012 | 22927404 |
| receptor diversity and host interaction of bacteriophages infecting salmonella enterica serovar typhimurium. | salmonella enterica subspecies enterica serovar typhimurium is a gram-negative pathogen causing salmonellosis. salmonella typhimurium-targeting bacteriophages have been proposed as an alternative biocontrol agent to antibiotics. to further understand infection and interaction mechanisms between the host strains and the bacteriophages, the receptor diversity of these phages needs to be elucidated. | 2012 | 22927964 |
| spontaneous and transient defence against bacteriophage by phase-variable glucosylation of o-antigen in salmonella enterica serovar typhimurium. | as natural killers of bacteria, bacteriophages have forced bacteria to develop a variety of defence mechanisms. the alteration of host receptors is one of the most common bacterial defence strategies against phage infection, which completely blocks phage attachment but comes at a potential fitness cost to the bacteria. here, we report the cost-free, transient emergence of phage resistance in salmonella enterica subspecies enterica serovar typhimurium through a phase-variable modification of the ... | 2012 | 22928771 |
| residual antibiotics disrupt meat fermentation and increase risk of infection. | fermented sausages, although presumed safe for consumption, sometimes cause serious bacterial infections in humans that may be deadly. not much is known about why and when this is the case. we tested the hypothesis that residual veterinary antibiotics in meat can disrupt the fermentation process, giving pathogenic bacteria a chance to survive and multiply. we found that six commercially available starter cultures were susceptible to commonly used antibiotics, namely, oxytetracycline, penicillin, ... | 2012 | 22930338 |
| the dub-ious lack of alis in salmonella infection: a salmonella deubiquitinase regulates the autophagy of protein aggregates. | ubiquitinated aggregates are formed in eukaryotic cells in response to several external stimuli, including exposure to bacterial lipopolysaccharide (lps). although salmonella enterica serovar typhimurium (s. typhimurium) lps has been shown to induce aggresome-like induced structures (alis) in macrophages, these have not been described in s. typhimurium-infected macrophages. given that lps is present in infection, this suggests that s. typhimurium might suppress the formation of alis. we found th ... | 2012 | 22931829 |
| salmonella enterica serovar agbeni, british columbia, canada, 2011. | 2012 | 22932699 | |
| whole-genome sequences and comparative genomics of salmonella enterica serovar typhi isolates from patients with fatal and nonfatal typhoid fever in papua new guinea. | many of the developing countries of the southeast asian region are significantly affected by endemic typhoid fever, possibly as a result of marginal living standards. it is an important public health problem in countries such as papua new guinea, which is geographically close to some of the foci of endemicity in asia. the severity of the disease varies in different regions, and this may be attributable to genetic diversity among the native strains. genome sequence data on strains from different ... | 2012 | 22933755 |
| insights from the genome sequence of a salmonella enterica serovar typhi strain associated with a sporadic case of typhoid fever in malaysia. | salmonella enterica serovar typhi is the causative agent of typhoid fever, which causes nearly 21.7 million illnesses and 217,000 deaths globally. herein, we describe the whole-genome sequence of the salmonella typhi strain st0208, isolated from a sporadic case of typhoid fever in kuala lumpur, malaysia. the whole-genome sequence and comparative genomics allow an in-depth understanding of the genetic diversity, and its link to pathogenicity and evolutionary dynamics, of this highly clonal pathog ... | 2012 | 22933756 |
| draft genome sequences of eight salmonella enterica serotype newport strains from diverse hosts and locations. | salmonellosis is a major contributor to the global public health burden. salmonella enterica serotype newport has ranked among three salmonella serotypes most commonly associated with food-borne outbreaks in the united states. it was thought to be polyphyletic and composed of independent lineages. here we report draft genomes of eight strains of s. newport from diverse hosts and locations. | 2012 | 22933769 |
| antibacterial effect of phosphates and polyphosphates with different chain length. | the aim of this study was to monitor the antibacterial effect of seven phosphate salts on selected strains of gram-negative and gram-positive bacteria, which could be considered responsible for food-borne diseases (bacillus cereus, bacillus subtilis, enterococcus faecalis, micrococcus luteus, staphylococcus aureus, citrobacter freundii, escherichia coli, proteus mirabilis, salmonella enterica ser. enteritidis and pseudomonas aeruginosa). for these purposes, phosphates differing in chain length w ... | 2012 | 22934995 |
| characterization and differential gene expression between two phenotypic phase variants in salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium strain 798 has previously been shown to undergo phenotypic phase variation. one of the phenotypes expresses virulence traits such as adhesion, while the other phenotype does not. phenotypic phase variation appears to correlate with the ability of this strain to cause persistent, asymptomatic infections of swine. a new method to detect cells in either phenotypic phase was developed using evans blue-uranine agar plates. using this new assay, rates of phenoty ... | 2012 | 22937065 |
| multiplex t-rflp allows for increased target number and specificity: detection of salmonella enterica and six species of listeria in a single test. | a multiplex t-rflp test was developed to detect and identify salmonella enterica and all six species of listeria inoculated into milk at minimal levels. extensive in silico analysis was used to design a fifteen-primer, six-amplimer methodology and in vitro application showed target organism dna, when amplified individually, yielded the predicted terminal restriction fragments (trfs) following digestion. non-target organisms were either not-amplified or yielded trfs which did not interfere with t ... | 2012 | 22937073 |
| [model of chronic salmonellosis: parameters of infection and immune response in inbred mice genetically variable in susceptibility to salmonellosis]. | study parameters of chronic infection and immune response in i/st and a/sn line mice in the model of per oral infection of mice with salmonella enterica serovar typhimurium. | 2013 | 22937698 |
| non-typhoidal salmonella encephalopathy involving lipopolysaccharide in cattle. | this study assessed the involvement of lipopolysaccharide (lps) in the non-typhoidal salmonella encephalopathy (ntse) caused by a unique isolate of salmonella enterica serovar saint-paul (sstpnpg). ntse was prevented by genetic (deletion of mure) or pharmacologic (polymyxin) disruption of lps on sstpnpg although the disruption of lps did not deter brain penetration of the strain. this is the first study to demonstrate that lps is involved in the manifestations of ntse. | 2013 | 22939987 |
| residual viral and bacterial contamination of surfaces after cleaning and disinfection. | environmental surfaces contaminated with pathogens can be sources of indirect transmission, and cleaning and disinfection are common interventions focused on reducing contamination levels. we determined the efficacy of cleaning and disinfection procedures for reducing contamination by noroviruses, rotavirus, poliovirus, parechovirus, adenovirus, influenza virus, staphylococcus aureus, and salmonella enterica from artificially contaminated stainless steel surfaces. after a single wipe with water, ... | 2012 | 22941071 |
| prevalence and relative risk of cronobacter spp., salmonella spp., and listeria monocytogenes associated with the body surfaces and guts of individual filth flies. | although flies are important vectors of food-borne pathogens, there is little information to accurately assess the food-related health risk of the presence of individual flies, especially in urban areas. this study quantifies the prevalence and the relative risk of food-borne pathogens associated with the body surfaces and guts of individual wild flies. one hundred flies were collected from the dumpsters of 10 randomly selected urban restaurants. flies were identified using taxonomic keys before ... | 2012 | 22941079 |
| global transcriptional analysis of dehydrated salmonella enterica serovar typhimurium. | despite the scientific and industrial importance of desiccation tolerance in salmonella, knowledge regarding its genetic basis is still scarce. in the present study, we performed a transcriptomic analysis of dehydrated and water-suspended salmonella enterica serovar typhimurium using microarrays. dehydration induced expression of 90 genes and downregulated that of 7 genes. ribosomal structural genes represented the most abundant functional group with a relatively higher transcription during dehy ... | 2012 | 22941081 |
| a dual tag system for facilitated detection of surface expressed proteins in escherichia coli. | the discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of escherichia coli. we have previously reported the use of the aida-i autotransport system to express the salmonella enterica serovar enteritidis proteins sefa and h:gm. the sefa protein was successfully exposed to the medium, but the orientation of h:gm in the outer membrane could not be determined due to proteolytic cleavage of the n-terminal detection-tag. the goal of ... | 2012 | 22943700 |
| comparative proteome and transcriptome analyses of wild-type and live vaccine strains of salmonella enterica serovar gallinarum. | salmonella enterica serovar gallinarum causes fowl typhoid in chickens and is of economic importance to the chicken industry. a serovar gallinarum live vaccine strain 9r (sg 9r) has been used to control fowl typhoid in many areas where the disease is endemic. because the attenuation mechanism of sg 9r was not defined, there has been continued concern about reversion to virulence. in this study, we examined the molecular characteristics, which might provide better insight into attenuation of sg 9 ... | 2012 | 22944630 |
| increasing quinolone resistance and multidrug resistant isolates among salmonella enterica in hong kong. | to study the antimicrobial susceptibility and molecular epidemiology of salmonella enterica isolates from 2005 to 2010 in hong kong. | 2012 | 22947244 |
| longitudinal dissemination of salmonella enterica clonal groups through the slaughter process of salmonella-positive pig batches. | this study was conducted to assess the dissemination of salmonella clonal groups in slaughterhouses that received batches of salmonella -positive pigs and used different routine processing procedures. eight serial sampling sessions were conducted in three slaughterhouses (a, b, and c). blood was collected randomly (n = 25) from each batch of pigs and processed for serology. carcasses (n = 12) were identified and sampled after dehairing, after singeing, after evisceration, and before chilling. a ... | 2012 | 22947464 |
| filter-based pathogen enrichment technology for detection of multiple viable foodborne pathogens in 1 day. | conventional foodborne pathogen assays currently used in the food industry often require long culture enrichments to increase pathogen levels so they can be detected. even using sensitive real-time pcr assays, culture enrichment at least overnight is necessary especially for detection of pathogens with slow growth rates such as listeria monocytogenes. to eliminate this cumbersome enrichment step and detect minute amounts of pathogens within 1 day, filter-based pathogen enrichment technology was ... | 2012 | 22947467 |
| regulation of rama by ramr in salmonella enterica serovar typhimurium: isolation of a ramr superrepressor. | rama is a transcription factor involved in regulating multidrug resistance in salmonella enterica serovar typhimurium sl1344. green fluorescent protein (gfp) reporter fusions were exploited to investigate the regulation of rama expression by ramr. we show that ramr represses the rama promoter by binding to a palindromic sequence and describe a superrepressor ramr mutant that binds to the rama promoter sequence more efficiently, thus exhibiting a rama inactivated phenotype. | 2012 | 22948865 |
| prevalence of salmonella cerro in laboratory-based submissions of cattle and comparison with human infections in pennsylvania, 2005-2010. | the aim of this study was to identify salmonella serotypes infecting cattle in pennsylvania, to compare infection rates for the predominant serotype, salmonella enterica serotype cerro, with the infection rates for the same serotype in humans, and to study the clonal diversity and antimicrobial resistance for this serotype in cattle from 2005 to 2010. clonal diversity among the selected isolates was studied using pulsed-field gel electrophoresis (pfge) and repetitive (rep)-polymerase chain react ... | 2012 | 22953739 |
| a novel salmonella serovar isolated from peregrine falcon (falco peregrinus) nestlings in sweden: salmonella enterica enterica serovar pajala (salmonella pajala). | a novel salmonella serovar was isolated from peregrine falcon (falco peregrinus) nestlings in northern sweden in 2006. three isolates of the same clone was retrieved from three falcon siblings and characterized as salmonella enterica sub-species enterica: o-phase 13, 23:-: e, n, z 15 and the h-phase was not present. we propose the geographical name salmonella enterica, sub-species enterica serovar pajala to this novel salmonella. | 2012 | 22957136 |
| tigecycline challenge triggers srna production in salmonella enterica serovar typhimurium. | bacteria employ complex transcriptional networks involving multiple genes in response to stress, which is not limited to gene and protein networks but now includes small rnas (srnas). these regulatory rna molecules are increasingly shown to be able to initiate regulatory cascades and modulate the expression of multiple genes that are involved in or required for survival under environmental challenge. despite mounting evidence for the importance of srnas in stress response, their role upon antibi ... | 2012 | 22958399 |
| salmonella enteritidis in shell eggs: evolving concerns and innovative control measures. | the issue of egg contamination with salmonella enterica serovar enteritidis rose to prominence several decades ago with increasing rate of infection around the world. recent outbreaks have assured that this problem maintains a place in the public consciousness. extensive research has been conducted to investigate the factors precipitating contamination events, their avoidance, and mitigation of the threat of contaminated eggs; consequently, regulations have been put in place to increase the safe ... | 2012 | 22958532 |
| analysis of thic variants in the context of the metabolic network of salmonella enterica. | in bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (hmp) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (air), a branch point metabolite of purine and thiamine biosynthesis. thic is a member of the radical s-adenosylmethionine (adomet) superfamily and catalyzes the complex chemical rearrangement of air to hmp-p. as reconstituted in vitro, the thic reaction requires adomet, air, and reductant. this study analyzed variants of thic in vivo and in vitro to probe the metaboli ... | 2012 | 22961850 |
| increased resistance to multiple antimicrobials and altered resistance gene expression in cmy-2-positive salmonella enterica following a simulated patient treatment with ceftriaxone. | salmonellosis is one of the most common causes of food-borne disease in the united states. increasing antimicrobial resistance and corresponding increases in virulence present serious challenges. currently, empirical therapy for invasive salmonella enterica infection includes either ceftriaxone or ciprofloxacin (e. l. hohmann, clin. infect. dis. 32:263-269, 2001). the bla(cmy-2) gene confers resistance to ceftriaxone, the antimicrobial of choice for pediatric patients with invasive salmonella en ... | 2012 | 22961898 |
| lack of direct effects of agrochemicals on zoonotic pathogens and fecal indicator bacteria. | agrochemicals, fecal indicator bacteria (fib), and pathogens frequently contaminate water simultaneously. no significant direct effects of fertilizer, atrazine, malathion, and chlorothalonil on the survival of escherichia coli, enterococcus faecalis, salmonella enterica, human polyomaviruses, and adenovirus were detected, supporting the assertion that previously observed effects of agrochemicals on fib were indirect. | 2012 | 22961900 |
| complete genome sequence of bacteriophage ssu5 specific for salmonella enterica serovar typhimurium rough strains. | salmonella enterica serovar typhimurium rough strain-specific phage ssu5 was isolated, and its whole genome was sequenced. the 103,229-bp-long double-stranded dna genome of ssu5 encodes 130 open reading frames with one trna for asparagine. genomic analysis revealed that ssu5 might be the phylogenetic origin of cryptic plasmid phcm2 harbored by salmonella typhi ct18. | 2012 | 22966187 |
| impact of maternally-derived antibodies against salmonella enterica serovar typhimurium on the bacterial load in suckling piglets. | the significance of maternal immunity against non-typhoid salmonella spp. acquired by piglets via colostrum and milk was evaluated in a salmonella enterica serovar typhimurium challenge experiment. piglets from sows vaccinated with an experimental inactivated vaccine exhibited high levels of serum immunoglobulins g and a against s. typhimurium 4 days after birth, just prior to experimental oral challenge. the s. typhimurium load in the ileal and caecal wall of piglets 3 days after experimental i ... | 2013 | 22967925 |
| mapping the aetiology of non-malarial febrile illness in southeast asia through a systematic review--terra incognita impairing treatment policies. | an increasing use of point of care diagnostic tests that exclude malaria, coupled with a declining malaria burden in many endemic countries, is highlighting the lack of ability of many health systems to manage other causes of febrile disease. a lack of knowledge of distribution of these pathogens, and a lack of screening and point-of-care diagnostics to identify them, prevents effective management of these generally treatable contributors to disease burden. while prospective data collection is v ... | 2012 | 22970193 |
| current fluoroquinolone susceptibility criteria for salmonella needs re-evaluation. | disc diffusion technique is the routine susceptibility testing procedure for isolates of enteric fever, the most common clinical diagnosis among febrile patients in nepal. | 2013 | 22971857 |
| phenotypic analysis of salmonella enterica serovar typhimurium rpoe mutants encoding rna polymerase extracytoplasmic stress response sigma factors σ(e) with altered promoter specificity. | we previously identified mutants in the rpoe gene of salmonella enterica serovar typhimurium (s. typhimurium) encoding rna polymerase extracytoplasmic stress response sigma factors σ(e) with altered promoter specificity. the replacement of the conserved r171 residue in the conserved region 4.2 of σ(e) by different amino acid residues exhibited different phenotypes. while r171a almost completely abolished sigma factor activity, r171g and r171c mutant changes imparted a relaxed recognition phenoty ... | 2013 | 22972381 |
| colistin resistance in escherichia coli and salmonella enterica strains isolated from swine in brazil. | reports about acquired resistance to colistin in different bacteria species are increasing, including e. coli of animal origin, but reports of resistance in wild s. enterica of different serotypes from swine are not found in the literature. results obtained with one hundred and twenty-six e. coli strains from diseased swine and one hundred and twenty-four s. enterica strains from diseased and carrier swine showed a frequency of 6.3% and 21% of colistin-resistant strains, respectively. when compa ... | 2012 | 22973166 |
| membrane topology of salmonella invasion protein sipb confers osmotolerance. | salmonella enterica serovar typhimurium is a major cause of human gastrointestinal illness worldwide. this pathogen can persist in a wide range of environments, making it of great concern to public health. here, we report that the salmonella pathogenicity island (spi)-1 effector protein sipb exhibits a membrane topology that confers bacterial osmotolerance. disruption of the sipb gene or the invg gene (spi-1 component) significantly reduced the osmotolerance of s. typhimurium lt2. biochemical as ... | 2012 | 22975346 |
| survival and growth of salmonella enterica serovar enteritidis in membrane-processed liquid egg white with ph, temperature, and storage conditions as controlling factors. | processing temperature and ph are known to influence the lethality and cell injury in many microbial interventions. a study was undertaken to determine the effects of variations in solution ph and process temperature on the removal and growth of salmonella enterica serovar enteritidis in liquid egg white (lew) by microfiltration (mf) membrane process. the effects of various storage conditions on the growth of salmonella in membrane-separated lew were evaluated. pretreated and ph-adjusted (ph 6 t ... | 2012 | 22980004 |
| ranking the disease burden of 14 pathogens in food sources in the united states using attribution data from outbreak investigations and expert elicitation. | understanding the relative public health impact of major microbiological hazards across the food supply is critical for a risk-based national food safety system. this study was conducted to estimate the u.s. health burden of 14 major pathogens in 12 broad categories of food and to then rank the resulting 168 pathogen-food combinations. these pathogens examined were campylobacter, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, norovirus, salmonella enterica, toxoplasma ... | 2012 | 22980012 |
| annual cost of illness and quality-adjusted life year losses in the united states due to 14 foodborne pathogens. | in this article we estimate the annual cost of illness and quality-adjusted life year (qaly) loss in the united states caused by 14 of the 31 major foodborne pathogens reported on by scallan et al. (emerg. infect. dis. 17:7-15, 2011), based on their incidence estimates of foodborne illness in the united states. these 14 pathogens account for 95 % of illnesses and hospitalizations and 98 % of deaths due to identifiable pathogens estimated by scallan et al. we estimate that these 14 pathogens caus ... | 2012 | 22980013 |
| subtype specific risk factor analyses for sporadic human salmonellosis: a case-case comparison in lower saxony, germany. | with the intention to deepen the knowledge of the vertical transmission of particular subtypes of salmonella enterica from "the stable to the table" a case1-case2 analysis in lower saxony, germany, was conducted. the data collection was based on standardised telephone interviews with 1741 salmonella case persons. single-factor-analyses revealed statistically significant associations between s. typhimurium infections and animal keeping (odds ratio (or): 1.4; 95%-confidence-interval (ci): 1.2-1.7) ... | 2013 | 22981706 |
| synthesis and characterization of vo(ii), co(ii), ni(ii), cu(ii) and zn(ii) complexes of chromone based azo-linked schiff base ligand. | azo-schiff-base complexes of vo(ii), co(ii), ni(ii), cu(ii) and zn(ii) have been synthesized and characterized by elemental analysis, ir, uv-vis, (1)h nmr, mass spectra, molar conductance, magnetic susceptibility measurement, electron spin resonance (epr), cv, fluorescence, nlo and sem. the conductance data indicate the nonelectrolytic nature of the complexes, except vo(ii) complex which is electrolytic in nature. on the basis of electronic spectra and magnetic susceptibility octahedral geometry ... | 2012 | 22982386 |
| reptile pets-associated salmonella enterica subspecies diarizonae gastroenteritis in a neonate. | 2012 | 22982955 | |
| discovery and biological characterization of geranylated rna in bacteria. | a general ms-based screen for unusually hydrophobic cellular small molecule-rna conjugates revealed geranylated rna in escherichia coli, enterobacter aerogenes, pseudomonas aeruginosa and salmonella enterica var. typhimurium. the geranyl group is conjugated to the sulfur atom in two 5-methylaminomethyl-2-thiouridine nucleotides. these geranylated nucleotides occur in the first anticodon position of trna(glu)(uuc), trna(lys)(uuu) and trna(gln)(uug) at a frequency of up to 6.7% (~400 geranylated n ... | 2012 | 22983156 |
| quantification of bacterial indicators and zoonotic pathogens in dairy wastewater ponds. | zoonotic pathogens in land-applied dairy wastewaters are a potential health risk. the occurrence and abundance of 10 pathogens and 3 fecal indicators were determined by quantitative real-time pcr (qpcr) in samples from 30 dairy wastewaters from southern idaho. samples tested positive for campylobacter jejuni, stx(1)- and eaea-positive escherichia coli, listeria monocytogenes, mycobacterium avium subsp. paratuberculosis, and salmonella enterica, with mean recoveries of genomic dna corresponding t ... | 2012 | 22983964 |
| characterization of lactic acid bacteria from naturally-fermented manzanilla aloreña green table olives. | manzanilla aloreña (or aloreña) table olives are naturally fermented traditional green olives with a denomination of protection (dop). the aim of this study was to search for lactic acid bacteria (lab) with technological properties of interest for possible inclusion in a starter or protective culture preparation or also as probiotics. a collection of 144 lab obtained from aloreña green table olives naturally-fermented by four small-medium enterprises (smes) from málaga (spain), including lactoba ... | 2012 | 22986194 |
| persistence of salmonella enterica during dehydration and subsequent cold storage. | despite the fact that salmonella enterica serotype typhimurium sl 1344 has served as a model pathogen in many studies, information regarding its desiccation response is still scarce. in this study, we investigated environmental conditions that affect salmonella survival following dehydration and subsequent cold storage, using a 96-well polystyrene plate model. the sl 1344 strain exhibited high survival compared with other typhimurium isolates and s. enterica serotypes. further characterization o ... | 2012 | 22986208 |
| [meningitis due to salmonella enterica]. | 2012 | 22987268 | |
| transposon mutagenesis of salmonella enterica serovar enteritidis identifies genes that contribute to invasiveness in human and chicken cells and survival in egg albumen. | salmonella enterica serovar enteritidis is an important food-borne pathogen, and chickens are a primary reservoir of human infection. while most knowledge about salmonella pathogenesis is based on research conducted on salmonella enterica serovar typhimurium, s. enteritidis is known to have pathobiology specific to chickens that impacts epidemiology in humans. therefore, more information is needed about s. enteritidis pathobiology in comparison to that of s. typhimurium. we used transposon mutag ... | 2012 | 22988017 |
| ygiw homologous gene from pseudomonas aeruginosa 25w is responsible for tributyltin resistance. | a tributyltin (tbt) resistance gene was isolated from the tbt-resistant marine origin bacterium pseudomonas aeruginosa 25w. this gene was identical to pa0320 deposited in the p. aeruginosa pao1 database (http://www.pseudomonas.com). the deduced amino acid sequence of pa0320 appears to be homologous to the ygiw proteins of escherichia coli and salmonella enterica. the deletion mutant of pa0320 showed a reduction of growth rate in the presence of tbt. a susceptibility test to cadmium, mercury, hyd ... | 2012 | 22990488 |
| immunization with outer membrane protein a from salmonella enterica serovar enteritidis induces humoral immune response but no protection against homologous challenge in chickens. | vaccination of poultry is one promising strategy to mitigate salmonella infection in poultry and, in turn, humans as well. we evaluated the efficacy of outer membrane protein a (ompa) as a novel vaccine candidate against salmonella in poultry. native ompa purified from salmonella enterica serovar enteritidis was mixed with adjuvant and administered intramuscularly to 41-d-old chicks. the vaccinated birds showed no decrease in cecal excretion and tissue colonization compared with the unvaccinated ... | 2012 | 22991526 |
| prevalence of salmonella enterica serovar 4,[5],12:i:- in england and wales, 2010. | difficulties in accurately identifying serovar 4,[5],12:i:- as monophasic variants of salmonella enterica serovar typhimurium mean there is confusion in the reporting of serovars typhimurium and 4,[5],12:i:-. to gain insight into the prevalence and diversity of these monophasic variants in england and wales, screening for fljb, hin and the serovar 4,[5],12:i:- dt193-associated genomic island was conducted on 609 s. enterica isolates designated as definitive phage type (dt) 193, and 142 isolates ... | 2012 | 22995432 |
| natural lysogenization and transduction in salmonella enterica serovar choleraesuis by bacteriophage p1. | it has been reported that bacteriophage p1 injects dna into serovar choleraesuis without evidence of productive infection. however, we found that p1 generates progeny and is capable of transduction in serovar choleraesuis. this is not the case with other serovars of salmonella enterica we tested. therefore, p1 could play a role in serovar choleraesuis evolution and contribute to its genetic manipulation and analysis. | 2012 | 22995628 |
| comparison of dkgb-linked intergenic sequence ribotyping to dna microarray hybridization for assigning serotype to salmonella enterica. | two dna-based methods were compared for the ability to assign serotype to 139 isolates of salmonella enterica ssp. i. intergenic sequence ribotyping (isr) evaluated single nucleotide polymorphisms occurring in a 5s ribosomal gene region and flanking sequences bordering the gene dkgb. a dna microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. serotype was assigned for 128 (92.1%) of submissions by the two dna methods. isr detected mixt ... | 2012 | 22998607 |
| prevalence of quinolone resistance and mutations in the topoisomerase genes in salmonella enterica serotype enteritidis isolates from serbia. | the prevalence of quinolone resistance was studied in salmonella enterica serotype enteritidis isolates collected during 2005-2010 in southern bačka county, serbia. a total of 878 clinical isolates were examined, among which 19 (2.2%) nalidixic acid (nal)-resistant s. enteritidis were detected by selection on agar plates containing 64 mg/l nal. antimicrobial susceptibility of the isolates was tested by the agar dilution method. according to clinical and laboratory standards institute (clsi) brea ... | 2012 | 22999768 |
| inactivation of salmonella enterica by uv-c light alone and in combination with mild temperatures. | the aim of this investigation was to study the efficacy of the combined processes of uv light and mild temperatures for the inactivation of salmonella enterica subsp. enterica and to explore the mechanism of inactivation. the doses to inactivate the 99.99% (4d) of the initial population ranged from 18.03 (salmonella enterica serovar typhimurium stcc 878) to 12.75 j ml(-1) (salmonella enterica serovar enteritidis atcc 13076). the ph and water activity of the treatment medium did not change the uv ... | 2012 | 23001665 |
| detection and identification of salmonella enterica, escherichia coli, and shigella spp. via pcr-electrospray ionization mass spectrometry: isolate testing and analysis of food samples. | an assay to identify the common food-borne pathogens salmonella, escherichia coli, shigella, and listeria monocytogenes was developed in collaboration with ibis biosciences (a division of abbott molecular) for the plex-id biosensor system, a platform that uses electrospray ionization mass spectroscopy (esi-ms) to detect the base composition of short pcr amplicons. the new food-borne pathogen (fbp) plate has been experimentally designed using four gene segments for a total of eight amplicon targe ... | 2012 | 23001674 |
| targeting essential genes in salmonella enterica serovar typhimurium with antisense peptide nucleic acid. | we investigated the capability of antisense peptide nucleic acids (pnas) conjugated to the (kff)(3)k cell-penetrating peptide to target possible essential genes (liga, rpoa, rpod, enga, tsf, and kdta) in salmonella enterica serovar typhimurium and inhibit bacterial growth in vitro and in cell culture. all targeted pna-based gene inhibition has shown great potency in gene expression inhibition in a sequence-specific and dose-dependent manner at micromolar concentrations. among tested pnas, the an ... | 2012 | 23006748 |
| expression of stm4467-encoded arginine deiminase controlled by the stm4463 regulator contributes to salmonella enterica serovar typhimurium virulence. | arginine deiminase (adi), carbamate kinase (ck), and ornithine transcarbamoylase (otc) constitute the adi system. in addition to metabolic functions, the adi system has been implicated in the virulence of certain pathogens. the pathogenic intracellular bacterium salmonella enterica serovar typhimurium possesses the stm4467, stm4466, and stm4465 genes, which are predicted to encode adi, ck, and otc, respectively. here we report that the stm4467 gene encodes an adi and that adi activity plays a ro ... | 2012 | 23006851 |
| effects of parabens and isothiazolinone on the microbiological quality of baby shampoo: the challenge test. | an in vitro microbial challenge test has been developed to predict the likelihood of consumer contamination of baby shampoo. four preservatives were tested in our study: the parbens medcide d, medcide pb, sepicide hb. and isothiazolinone methylisothiazolinone/chloromethylisothiazolinone [mi/mci]. these preservatives were tested separately and in combination. the challenge test involved inoculating the product with micrococcus luteus, staphylococcus aureus, escherichia coli, salmonella enterica, ... | 2012 | 23007105 |
| occurrence and characterization of monophasic salmonella enterica serovar typhimurium (1,4,[5],12:i:-) of non-human origin in poland. | the epidemiological role of monophasic salmonella enterica subsp. enterica serovar typhimurium tends to increase, indicating pandemic spread. the aim of the present study was to confirm the occurrence of this serological variant in poland and to report the first cases in belarus and ukraine. genetic similarity of monophasic isolates with salmonella typhimurium already present in these countries was assessed. serotyping, duplex-polymerase chain reaction (pcr) assay, antibiotic resistance and puls ... | 2012 | 23009171 |
| engineering of acetyl-coa metabolism for the improved production of polyhydroxybutyrate in saccharomyces cerevisiae. | through metabolic engineering microorganisms can be engineered to produce new products and further produce these with higher yield and productivities. here, we expressed the bacterial polyhydroxybutyrate (phb) pathway in the yeast saccharomyces cerevisiae and we further evaluated the effect of engineering the formation of acetyl coenzyme a (acetyl-coa), an intermediate of the central carbon metabolism and precursor of the phb pathway, on heterologous phb production by yeast. we engineered the ac ... | 2012 | 23009357 |
| salmonella enterica subsp. enterica isolated from chicken carcasses and environment at slaughter in reunion island: prevalence, genetic characterization and antibiotic susceptibility. | salmonella contamination of 71 chicken broiler flocks was investigated at the slaughterhouse in reunion island between october 2007 and january 2009. samples were collected from live broiler chickens and chicken carcasses as well as the slaughterhouse environment. salmonella spp. was isolated from 40 of 71 (56 % with a confidence interval 5 % [45-67]) broiler chicken flocks at slaughter. the most prominent serovars were blockley (31 %), typhimurium and brancaster (14 %), hadar (10 %), salmonella ... | 2013 | 23011670 |
| functional characterization of quinolone-resistant mechanisms in a lab-selected salmonella enterica typhimurium mutant. | correlation has been widely accepted between quinolone resistance and topoisomerase point mutations in quinolone resistance determination regions (qrdrs). acquirement of point mutations in qrdrs usually increases the microbial resistance to both nalidixic acid and fluoroquinolones. the quinolone-resistant mechanisms accumulated in a lab-selected mutant were characterized through the construction of isogenic mutants using phage λ red recombinase system and phage p22. the function of a quinolone-r ... | 2013 | 23013383 |
| multidrug-resistant salmonella enterica, democratic republic of the congo. | 2012 | 23017665 | |
| the pmrab system-inducing conditions control both lipid a remodeling and o-antigen length distribution, influencing the salmonella typhimurium-host interactions. | the salmonella enterica serovar typhimurium lipopolysaccharide consisting of covalently linked lipid a, non-repeating core oligosaccharide, and the o-antigen polysaccharide is the most exposed component of the cell envelope. previous studies demonstrated that all of these regions act against the host immunity barrier. the aim of this study was to define the role and interaction of pmrab-dependent gene products required for the lipopolysaccharide component synthesis or modification mainly during ... | 2012 | 23019341 |
| studies on the substrate specificity of a gdp-mannose pyrophosphorylase from salmonella enterica. | a series of methoxy and deoxy derivatives of mannopyranose-1-phosphate (manp-1p) were chemically synthesized, and their ability to be converted into the corresponding guanosine diphosphate mannopyranose (gdp-manp) analogues by a pyrophosphorylase (gdp-manpp) from salmonella enterica was studied. evaluation of methoxy analogues demonstrated that gdp-manpp is intolerant of bulky substituents at the c-2, c-3, and c-4 positions, in turn suggesting that these positions are buried inside the enzyme ac ... | 2012 | 23019451 |
| development of a custom 16s rrna gene library for the identification and molecular subtyping of salmonella enterica. | the use of 16s rrna gene sequencing within the regulatory workflow may help to reduce the time and labor involved in the identification and differentiation of salmonella enterica isolates. however, a comprehensive, standardized reference library is needed in order to use this method with regulatory samples. the goal of this project was to acquire 16s rrna partial and full gene sequences for a variety of s. enterica isolates and to use these sequences to build a custom 16s rrna reference library. ... | 2012 | 23022443 |
| intracontinental spread of human invasive salmonella typhimurium pathovariants in sub-saharan africa. | a highly invasive form of non-typhoidal salmonella (ints) disease has recently been documented in many countries in sub-saharan africa. the most common salmonella enterica serovar causing this disease is typhimurium (salmonella typhimurium). we applied whole-genome sequence-based phylogenetic methods to define the population structure of sub-saharan african invasive salmonella typhimurium isolates and compared these to global salmonella typhimurium populations. notably, the vast majority of sub- ... | 2012 | 23023330 |
| effect of frequency and waveform on inactivation of escherichia coli o157:h7 and salmonella enterica serovar typhimurium in salsa by ohmic heating. | the effect of frequency of alternating current during ohmic heating on electrode corrosion, heating rate, inactivation of food-borne pathogens, and quality of salsa was investigated. the impact of waveform on heating rate was also investigated. salsa was treated with various frequencies (60 hz to 20 khz) and waveforms (sine, square, and sawtooth) at a constant electric field strength of 12.5 v/cm. electrode corrosion did not occur when the frequency exceeded 1 khz. the heating rate of the sample ... | 2013 | 23023752 |
| interplay between the qsec and qsee bacterial adrenergic sensor kinases in salmonella enterica serovar typhimurium pathogenesis. | the bacterial adrenergic sensor kinases qsec and qsee respond to epinephrine and/or norepinephrine to initiate a complex phosphorelay regulatory cascade that modulates virulence gene expression in several pathogens. we have previously shown that qsec activates virulence gene expression in salmonella enterica serovar typhimurium. here we report the role of qsee in s. typhimurium pathogenesis as well as the interplay between these two histidine sensor kinases in gene regulation. an s. typhimurium ... | 2012 | 23027532 |
| microbial amyloids induce interleukin 17a (il-17a) and il-22 responses via toll-like receptor 2 activation in the intestinal mucosa. | the toll-like receptor 2 (tlr2)/tlr1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla firmicutes, bacteroidetes, and proteobacteria. to determine whether this tlr2/tlr1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, we investigated whether expression of curli amyloid fibrils by the invasive enteric pathogen salmonella enterica serotype typhimurium contributes to t helper 1 and t helper 17 respon ... | 2012 | 23027540 |
| very long o-antigen chains enhance fitness during salmonella-induced colitis by increasing bile resistance. | intestinal inflammation changes the luminal habitat for microbes through mechanisms that have not been fully resolved. we noticed that the fepe regulator of very long o-antigen chain assembly in the enteric pathogen salmonella enterica serotype typhimurium (s. typhimurium) conferred a luminal fitness advantage in the mouse colitis model. however, a fepe mutant was not defective for survival in tissue, resistance to complement or resistance to polymyxin b. we performed metabolite profiling to ide ... | 2012 | 23028318 |
| fruit, a scar-free system for targeted chromosomal mutagenesis, epitope tagging, and promoter replacement in escherichia coli and salmonella enterica. | recombineering is a widely-used approach to delete genes, introduce insertions and point mutations, and introduce epitope tags into bacterial chromosomes. many recombineering methods have been described, for a wide range of bacterial species. these methods are often limited by (i) low efficiency, and/or (ii) introduction of "scar" dna into the chromosome. here, we describe a rapid, efficient, pcr-based recombineering method, fruit, that can be used to introduce scar-free point mutations, deletio ... | 2012 | 23028641 |
| a naturally occurring single nucleotide polymorphism in the salmonella spi-2 type iii effector srfh/ssei controls early extraintestinal dissemination. | cd18 expressing phagocytes associated with the gastro-intestinal (gi) epithelium can shuttle salmonella directly into the bloodstream within a few minutes following microbial ingestion. we have previously demonstrated that salmonella controls the cd18 pathway to deeper tissue, manipulating the migratory properties of infected cells as an unappreciated component of its pathogenesis. we have observed that one type iii effector, srfh (also called ssei) that salmonella secretes into infected phagocy ... | 2012 | 23028876 |
| pathogenicity of salmonella enterica in caenorhabditis elegans relies on disseminated oxidative stress in the infected host. | feeding caenorhabditis elegans with salmonella enterica serovar typhimurium significantly shortens the lifespan of the nematode. s. typhimurium-infected c. elegans, stained with 2',7'-dichlorodihydrofluorescein diacetate which fluoresces upon exposure to reactive oxygen species, revealed intestinal luminal staining that along with the time of infection progressed to a strong staining in the hypodermal tissues of the nematode. still, we could not detect invasion beyond the nematode's intestinal e ... | 2012 | 23028994 |
| molecular characterization of the viab locus encoding the biosynthetic machinery for vi capsule formation in salmonella typhi. | the vi capsular polysaccharide (cps) of salmonella enterica serovar typhi, the cause of human typhoid, is important for infectivity and virulence. the vi biosynthetic machinery is encoded within the viab locus composed of 10 genes involved in regulation of expression (tvia), polymer synthesis (tvib-tvie), and cell surface localization of the cps (vexa-vexe). we cloned the viab locus from s. typhi and transposon insertion mutants of individual viab genes were characterized in escherichia coli dh5 ... | 2012 | 23029132 |
| on-cell mas nmr: physiological clues from living cells. | while structural information on biomolecules is mainly obtained from purified in vitro samples, nmr can also be applied in the context of entire cells or organisms. the present study describes maturation processes in living salmonella enterica serovar typhimurium, a prevalent cause for human gastroenteritis. in our physiological study, we follow the composition of the o-antigen on the outer bacterial membrane with high-resolution mas nmr spectroscopy. we detect and characterize an evolution of t ... | 2012 | 23030432 |
| a connecter-like factor, caca, links rssb/rpos and the cpxr/cpxa two-component system in salmonella. | bacteria integrate numerous environmental stimuli when generating cellular responses. increasing numbers of examples describe how one two-component system (tcs) responds to signals detected by the sensor of another tcs. however, the molecular mechanisms underlying this phenomenon remain poorly defined. | 2012 | 23031642 |
| salmonella enterica subsp. enterica producing vim-1 carbapenemase isolated from livestock farms. | 2013 | 23034713 | |
| effect of x-ray treatments on pathogenic bacteria, inherent microbiota, color, and texture on parsley leaves. | this work is a part of systematic studies of the effect of x-ray treatments on fresh produce. the main objective of this investigation was to study the effects of x-ray treatments in reducing the concentration of artificially inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica, and shigella flexneri, and inherent microbiota on parsley leaves. the secondary objective was to study the effects of x-ray treatments on color and texture parameters on treated parsley leaves ... | 2012 | 23035726 |
| prevalence of salmonella spp. in cane toads (bufo marinus) from grenada, west indies, and their antimicrobial susceptibility. | cloacal swabs and caecal contents sampled from 58 cane toads (bufo marinus) in st george's parish, grenada, during a 7-month period in 2011 were examined by an enrichment and selective culture method for presence of salmonella spp. twenty-four (41%) toads were positive for salmonella spp. of which eight were salmonella enterica serovar javiana, and eight were s. enterica serovar rubislaw. the other serovars were as follows: montevideo, 6; arechavaleta, 1; and serovar: iv:43:-:-, 1. the high freq ... | 2013 | 23035820 |
| structural insights into the function of the nicotinate mononucleotide:phenol/p-cresol phosphoribosyltransferase (arsab) enzyme from sporomusa ovata. | cobamides (cbas) are cobalt (co) containing tetrapyrrole-derivatives involved in enzyme-catalyzed carbon skeleton rearrangements, methyl-group transfers, and reductive dehalogenation. the biosynthesis of cobamides is complex and is only performed by some bacteria and achaea. cobamides have an upper (coβ) ligand (5'-deoxyadenosyl or methyl) and a lower (coα) ligand base that contribute to the axial co coordinations. the identity of the lower coα ligand varies depending on the organism synthesizin ... | 2012 | 23039029 |
| evolution of transcriptional regulation in closely related bacteria. | the exponential growth of the number of fully sequenced genomes at varying taxonomic closeness allows one to characterize transcriptional regulation using comparative-genomics analysis instead of time-consuming experimental methods. a transcriptional regulatory unit consists of a transcription factor, its binding site and a regulated gene. these units constitute a graph which contains so-called "network motifs", subgraphs of a given structure. here we consider genomes of closely related enteroba ... | 2012 | 23039862 |
| interplay between fur and hns in controlling virulence gene expression in salmonella typhimurium. | salmonella enterica is responsible for a large number of diseases in a wide-range of hosts. two of the global regulators involved in controlling gene expression during the infection cycle of the bacterium are fur and hns. in this paper, we demonstrate computationally that fur and hns have disproportionately high density of binding sites in the pathogenicity islands on the salmonella chromosome. moreover, the frequency of binding sites for the two proteins is correlated throughout the genome of t ... | 2012 | 23040276 |
| genetic manipulation of pathogenicity loci in non-typhimurium salmonella. | the traditional genetic tools used in salmonella enterica serovar typhimurium rely heavily on a high-transducing mutant of bacteriophage p22. p22 recognizes its hosts by the structure of their o-antigens, which vary among serovars of salmonella; therefore, it cannot be used in most non-typhimurium salmonella, including the majority of those causing food-borne illnesses in both humans and livestock. bacteriophage p1 infects a variety of enteric bacteria, including gale mutants of serovar typhimur ... | 2012 | 23041268 |
| population genetic structure of 4,12:a:- salmonella enterica strains from harbor porpoises. | according to pulsed-field gel electrophoresis (pfge) typing, 4,12:a:- salmonella enterica isolates from harbor porpoises are highly diverse. however, porpoise isolates belong to only two multilocus sequence types within the eburst group 18 (ebg18) genetic cluster, which also includes s. enterica serovars bispebjerg and abortusequi. isolates of other, serologically similar serovars belong to unrelated ebgs. these assignments to ebgs were supported by ebg-specific sequences of the flagellar gene f ... | 2012 | 23042176 |
| typhoid and paratyphoid fever in western sydney local health district, nsw, january-june 2011. | we undertook a study of enteric fever, caused by salmonella enterica enterica subtypes typhi and paratyphi a, presenting in residents of the western sydney local health district for the period january-june 2011. twelve cases of s. typhi and eight of s. paratyphi a were notified. patients were predominantly young adults (median age 26 years, 70% female) who had been visiting friends and relatives in india, samoa, bangladesh or sri lanka. no cases were associated with travel for less than 3 weeks; ... | 2012 | 23043747 |
| effect of chemical sanitizers on salmonella enterica serovar poona on the surface of cantaloupe and pathogen contamination of internal tissues as a function of cutting procedure. | survival of salmonella enterica subsp. enterica serovar poona on surface and stem scar portions of inoculated cantaloupe following sanitizer application, transfer of pathogen from the rind to the flesh during cutting, and growth of salmonella poona on cantaloupe cubes over 15 days of refrigerated storage were investigated. cantaloupes inoculated with a rifampin-resistant strain of salmonella poona (10(7) cfu/ml) for 3 min and dried for 12 h were washed with chlorine (200 mg free chlorine per lit ... | 2012 | 23043824 |
| genome sequence and comparative pathogenomics analysis of a salmonella enterica serovar typhi strain associated with a typhoid carrier in malaysia. | salmonella enterica serovar typhi is a human pathogen that causes typhoid fever predominantly in developing countries. in this article, we describe the whole genome sequence of the s. typhi strain cr0044 isolated from a typhoid fever carrier in kelantan, malaysia. these data will further enhance the understanding of its host persistence and adaptive mechanism. | 2012 | 23045488 |
| draft genome sequences of 21 salmonella enterica serovar enteritidis strains. | salmonella enterica subsp. enterica serovar enteritidis is a common food-borne pathogen, often associated with shell eggs and poultry. here, we report draft genomes of 21 s. enteritidis strains associated with or related to the u.s.-wide 2010 shell egg recall. eleven of these genomes were from environmental isolates associated with the egg outbreak, and 10 were reference isolates from previous years, unrelated to the outbreak. the whole-genome sequence data for these 21 human pathogen strains ar ... | 2012 | 23045502 |
| human infections due to salmonella blockley, a rare serotype in south africa: a case report. | infections due to nontyphoidal salmonella have increased worldwide over the last couple of decades. salmonella enterica serotype blockley (salmonella blockley) infections is associated with chickens and is a rarely isolated serotype in human infections in most countries. | 2012 | 23050633 |
| bacterial coinfections in travelers with malaria: rationale for antibiotic therapy. | malaria predisposes children in areas where malaria is endemic to concurrent bacteremia, often with severe outcomes. the importance of bacterial coinfections in patients diagnosed with malaria in nonendemic settings has, however, not been reported. a retrospective analysis of microbiology data was performed in 755 travelers diagnosed with malaria in sweden. bacterial cultures from blood and other locations were correlated to clinical outcome and antibiotic treatment. blood cultures were drawn fr ... | 2013 | 23052321 |
| engineering, conjugation, and immunogenicity assessment of escherichia coli o121 o antigen for its potential use as a typhoid vaccine component. | state-of-the-art production technologies for conjugate vaccines are complex, multi-step processes. an alternative approach to produce glycoconjugates is based on the bacterial n-linked protein glycosylation system first described in campylobacter jejuni. the c. jejuni n-glycosylation system has been successfully transferred into escherichia coli, enabling in vivo production of customized recombinant glycoproteins. however, some antigenic bacterial cell surface polysaccharides, like the vi antige ... | 2013 | 23053636 |
| host-pathogen interaction in invasive salmonellosis. | salmonella enterica infections result in diverse clinical manifestations. typhoid fever, caused by s. enterica serovar typhi (s. typhi) and s. paratyphi a, is a bacteremic illness but whose clinical features differ from other gram-negative bacteremias. non-typhoidal salmonella (nts) serovars cause self-limiting diarrhea with occasional secondary bacteremia. primary nts bacteremia can occur in the immunocompromised host and infants in sub-saharan africa. recent studies on host-pathogen interactio ... | 2012 | 23055923 |
| fast dna serotyping and antimicrobial resistance gene determination of salmonella enterica with an oligonucleotide microarray-based assay. | salmonellosis caused by salmonella (s.) belongs to the most prevalent food-borne zoonotic diseases throughout the world. therefore, serotype identification for all culture-confirmed cases of salmonella infection is important for epidemiological purposes. as a standard, the traditional culture method (iso 6579:2002) is used to identify salmonella. classical serotyping takes 4-5 days to be completed, it is labor-intensive, expensive and more than 250 non-standardized sera are necessary to characte ... | 2012 | 23056321 |