Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of food processing-related stresses on acid tolerance of listeria monocytogenes. | stationary-phase cells of listeria monocytogenes grown in glucose-free or glucose-containing media were exposed for 90 min to various stresses, including acid stress (ph 4.0 to 7.0), osmotic stress (10.5 to 20.5% nacl), and various temperatures (-5 to 50 degrees c), and were further exposed to ph 3.5. exposure to a mildly acidic (ph 5.0 to 6.0) environment provided protection of the pathogen against acid upon subsequent exposure. this adaptive response, however, was found to be strongly dependen ... | 2003 | 14660405 |
| structural aspects of adhesion to and invasion of host cells by the human pathogen listeria monocytogenes. | 2003 | 14661268 | |
| the interaction of gamma delta t cells with activated macrophages is a property of the v gamma 1 subset. | immunoregulation is an emerging paradigm of gammadelta t cell function. the mechanisms by which gammadelta t cells mediate this function, however, are not clear. studies have identified a direct role for gammadelta t cells in resolving the host immune response to infection, by eliminating populations of activated macrophages. the aim of this study was to identify macrophage-reactive gammadelta t cells and establish the requirements/outcomes of macrophage-gammadelta t cell interactions during the ... | 2003 | 14662848 |
| listeria monocytogenes serotype identification by pcr. | serotyping is a universally accepted subtyping method for listeria monocytogenes. identification of the strain serotype permits differentiation between important food-borne strains (1/2a, 1/2b, and 4b) and provides a "gold standard" for comparing isolates analyzed in different labs and with different techniques. although an efficient enzyme-linked immunosorbent assay serotyping protocol was described recently, identification of pcr serotyping primers would further increase the ease and accessibi ... | 2003 | 14662936 |
| the effect of diffusion, depolymerization and nucleation promoting factors on actin gel growth. | in eukaryotic cells, localized actin polymerization is able to deform the plasma membrane and push the cell forward. depolymerization of actin filaments and diffusion of actin monomers ensure the availability of monomers at sites of polymerization, and therefore these processes must play an active role in cellular actin dynamics. here we reveal experimental evidence that actin gel growth can be limited by monomer diffusion, consistent with theoretical predictions. we study actin gels formed on b ... | 2004 | 14663631 |
| characterisation of persistent and sporadic listeria monocytogenes strains by pulsed-field gel electrophoresis (pfge) and amplified fragment length polymorphism (aflp). | this study was set up to evaluate the genetic similarity or dissimilarity of persistent and sporadic listeria monocytogenes strains existing in eleven food processing facilities, including fish, dairy, meat and poultry processing plants. in each plant persistent and sporadic strains were selected on the basis of pfge typing results. a total of 17 strains representing persistent strains and 38 sporadic strains originating from eleven food processing plants were included in the study. pfge macrore ... | 2003 | 14666982 |
| oral (gavage), in utero and post-natal exposure of sprague-dawley rats to low doses of tributyltin chloride. part ii: effects on the immune system. | the immunotoxic effects of tributyltin chloride (tbtc) were examined in the offspring of sprague-dawley rats exposed in utero from day 8 of gestation, through lactation and post-weaning until pups reached the age of 30 days (male and female), 60 days (female) and 90 days (male). daily oral (gavage) doses of 0.025, 0.25 and 2.5 mg/kg body weight/day were administered in olive oil 7 days/week. immunologic endpoints were investigated at the termination of each study. statistically significant resul ... | 2004 | 14667469 |
| periprosthetic knee joint infection following colonoscopy. a case report. | 2003 | 14668516 | |
| phosphorylation of the stat1 transactivation domain is required for full-fledged ifn-gamma-dependent innate immunity. | stat1 is phosphorylated on serine 727 within its transactivating domain (tad) in response to interferons or other immunological signals. we generated gene-targeted mutant mice expressing a serine727-alanine mutant of stat1. these animals showed increased mortality upon infection with listeria monocytogenes and impaired clearance of the bacteria from spleen and liver. the stat1s727a mice were more resistant to the lps-induced septic shock syndrome, suggesting that stat1 serine phosphorylation pro ... | 2003 | 14670297 |
| sequential myd88-independent and -dependent activation of innate immune responses to intracellular bacterial infection. | microbial infections induce chemokine and cytokine cascades that coordinate innate immune defenses. infection with the intracellular bacterial pathogen listeria monocytogenes induces ccr2-dependent monocyte recruitment and activation, an essential response for host survival. herein we show that invasive l. monocytogenes, but not killed or noninvasive bacteria, induce secretion of mcp-1, the requisite chemokine for monocyte recruitment. induction of mcp-1, but not tnf or il-12, following l. monoc ... | 2003 | 14670305 |
| survival and growth of listeria monocytogenes and enterohemorrhagic escherichia coli o157:h7 in minimally processed artichokes. | the ability of listeria monocytogenes and escherichia coli o157:h7 inoculated by immersion (at 4.6 and 5.5 log cfu/ g, respectively) to survive on artichokes during various stages of preparation was determined. peeling, cutting, and disinfecting operations (immersion in 50 ppm of a free chlorine solution at 4 degrees c for 5 min) reduced populations of l. monocytogenes and e. coli o157:h7 by only 1.6 and 0.8 log units, respectively. an organic acid rinse (0.02% citric acid and 0.2% ascorbic acid ... | 2003 | 14672214 |
| nitrite-induced injury of listeria monocytogenes and the effect of selective versus nonselective recovery procedures on its isolation from frankfurters. | sodium nitrite (nano2) is used as a curing agent in frankfurters. although previous studies have documented the bacteriostatic abilities of nano2 toward listeria monocytogenes, few if any studies have been conducted that consider the possibility of sublethal injury to l. monocytogenes by exposure to nano2. the goals of this study were to determine whether nano2 has the ability to injure l. monocytogenes, to determine whether nitrite injury is reversible, and to compare the recovery of l. monocyt ... | 2003 | 14672221 |
| microbiology of charcoal-broiled european river lampreys (lampetra fluviatilis) stored at 3 and 22 degrees c. | the microbiological quality of 30 production lots of charcoal-broiled river lampreys was studied at three lamprey processing plants (plants a, b, and c). samples were taken directly after charcoal broiling and stored at 22 and 3 degrees c. lampreys were examined on the day of manufacture, and those kept at 22 degrees c were examined every second day for 6 days. samples kept at 3 degrees c were examined every fourth day for up to 24 days. on the production day, the mean aerobic plate counts (apcs ... | 2003 | 14672233 |
| pathogenicity of food and clinical listeria monocytogenes isolates in a mouse bioassay. | serotype distributions of listeria monocytogenes in clinical samples and foods often differ. it is unknown whether such differences reflect a variation in the virulence of strains or are due to other factors that are not directly related to the strains' ability to cause illnesses. fifty-two food and eight clinical isolates of l. monocytogenes were obtained from france, japan, and the united states. their pathogenicity in nonimmunocompromised female icr mice was determined by intraperitoneal (i.p ... | 2003 | 14672239 |
| identification of novel eubacteria from spent mushroom compost (smc) waste by dna sequence typing: ecological considerations of disposal on agricultural land. | a small study was undertaken to examine the microbiological characteristics of spent mushroom compost (smc), which is the major waste by-product of the mushroom industry and which is regularly disposed off by application to agricultural land. the primary aim of this study was to examine smc for the presence of faecal bacterial pathogens, including campylobacter spp., salmonella spp. and listeria monocytogenes. secondly it was desirable to quantify bacterial and fungal populations within smc, and ... | 2004 | 14672727 |
| effective control of listeria monocytogenes by combination of nisin formulated and slowly released into a broth system. | in order to identify conditions for efficient food preservation by nisin, the sensitivity of listeria monocytogenes to this preservative was studied under the following three model conditions: (1) the instantaneous addition of nisin into broth medium to simulate the formation of nisin in foods, (2) the slow delivery of nisin solution into broth medium using a pump to simulate the slow release of nisin from packaging materials to foods, (3) a combination of the two delivery methods. based on the ... | 2004 | 14672827 |
| enhancement of nisin, lysozyme, and monolaurin antimicrobial activities by ethylenediaminetetraacetic acid and lactoferrin. | a microtiter plate assay was employed to systematically assess the interaction between ethylenediaminetetraacetic acid (edta) or lactoferrin and nisin, lysozyme, or monolaurin against strains of listeria monocytogenes, escherichia coli, salmonella enteritidis, and pseudomonas fluorescens. low levels of edta acted synergistically with nisin and lysozyme against l. monocytogenes but edta and monolaurin interacted additively against this microorganism. edta synergistically enhanced the activity of ... | 2004 | 14672831 |
| in vitro activity of naturally occurring peptides (defensins) against listeria monocytogenes. | autoclaved distilled water samples were inoculated with l. monocytogenes strain v7 and strain vph-1, and incubated aerobically, at 30 c for 48 hours. each strain was tested individually, and growth curves were determined at 1, 2, 3, 4, 5, 21, 24, and 48 hours. the growth or survival of l. monocytogenes was similar for both strains, with survivors at 24 hour-incubation. the microbicidal activity of one synthetic cationic peptide (np-2) was examined against l. monocytogenes strain v7, in a water s ... | 2003 | 14676929 |
| mechanistic diversity of fosfomycin resistance in pathogenic microorganisms. | microbial resistance to the antibiotic fosfomycin [(1r,2s)-epoxypropylphosphonic acid, 1] is known to be mediated by thiol transferase enzymes fosa and fosb, which catalyze the addition of glutathione and l-cysteine to c1 of the oxirane, respectively. a probe of the microbial genome database reveals a related group of enzymes (fosx). the genes mlr3345 from mesorhizobium loti and lmo1702 from listeria monocytogenes were cloned and the proteins expressed. this heretofore unrecognized group of enzy ... | 2003 | 14677948 |
| modelling and predicting the simultaneous growth of listeria monocytogenes and spoilage micro-organisms in cold-smoked salmon. | to evaluate and model the simultaneous growth of listeria monocytogenes and spoilage micro-organisms in cold-smoked salmon. | 2004 | 14678163 |
| the contribution of bacteriocin to inhibition of listeria monocytogenes by carnobacterium piscicola strains in cold-smoked salmon systems. | to study the importance of bacteriocin production for the antilisterial effect of a bacteriocinogenic carnobacterium piscicola strain a9b on growth of listeria monocytogenes in broth and cold-smoked salmon systems. | 2004 | 14678166 |
| effect of diesel exhaust particulate (dep) on immune responses: contributions of particulate versus organic soluble components. | the effect of diesel exhaust particulate (dep) exposure on innate, cellular and humoral pulmonary immunity was studied using high-dose, acute-exposure rat, mouse, and cell culture models. dep consists of a complex mixture of petrochemical-derived organics adsorbed onto elemental carbon particles. dep is a major component of particulate urban air pollution and a health concern in both urban and occupational environments. the alveolar macrophage is considered a key cellular component in pulmonary ... | 2004 | 14681077 |
| soluble metals associated with residual oil fly ash increase morbidity and lung injury after bacterial infection in rats. | inhalation of residual oil fly ash (rofa) has been shown to impair lung defense mechanisms in laboratory animals and susceptible populations. bioavailability of soluble transition metals has been shown to play a key role in lung injury caused by rofa exposure. the goal of this study was to evaluate the effect of soluble metals on lung defense and injury in animals preexposed to rofa followed by pulmonary challenge with a bacterial pathogen. rofa was suspended in saline (rofa-total), incubated ov ... | 2004 | 14681079 |
| [construction and verification of the effectiveness of pmbl: a cloning vector of exported proteins encoding genes]. | the beta-lactamase was used as the reporter of expression and transmembrane secretion in this paper. a fragment of amp resistance gene encoding the mature part of beta-lactamase (delta p delta sp amp, i.e. without promoter and signal peptide coding sequences) was amplified from puc18 vector. the upstream primer has bglii, bcli, bamhi in three reading frame respectively, in order to in frame fuse and express target genes together with the downstream reporter in finally constructed vector. meanwhi ... | 2003 | 14682241 |
| listeriosis in the pregnant guinea pig: a model of vertical transmission. | feto-placental infections represent a major cause of pregnancy complications, and yet the underlying molecular and cellular mechanisms of vertical transmission are poorly understood. listeria monocytogenes, a facultative intracellular pathogen, is one of a group of pathogens that are known to cause feto-placental infections in humans and other mammals. the purpose of this study was to evaluate possible mechanisms of vertical transmission of l. monocytogenes. humans and guinea pigs have a hemocho ... | 2004 | 14688130 |
| detection of bacteria in suspension by using a superconducting quantum interference device. | we demonstrate a technique for detecting magnetically labeled listeria monocytogenes and for measuring the binding rate between antibody-linked magnetic particles and bacteria. this sensitive assay quantifies specific bacteria in a sample without the need to immobilize them or wash away unbound magnetic particles. in the measurement, we add 50-nm-diameter superparamagnetic magnetite particles, coated with antibodies, to an aqueous sample containing l. monocytogenes. we apply a pulsed magnetic fi ... | 2004 | 14688406 |
| an essential role of th1 responses and interferon gamma in infection-mediated suppression of neoplastic growth. | we had previously demonstrated that in mice acute toxoplasmosis leads to systemic inhibition of angiogenesis and, consequently, strong suppression of neoplastic growth. here we investigated the role of th1 cytokines, in particular interferon gamma (ifn-gamma), in this phenomenon. besides toxoplasma, neoplastic growth was readily blocked during acute infection with other th1 response-inducing pathogens such as listeria monocytogenes and lymphocytic choriomeningitis virus (lcmv). in contrast, chro ... | 2003 | 14688478 |
| isolation of pathogenic listeria monocytogenes and detection of antibodies against phosphatidylinositol-specific phospholipase c in buffaloes. | the isolation of pathogenic listeria spp. in bacteriological samples, and anti-phosphatidylinositol-specific phospholipase c (anti-piplc) antibodies in sera of buffaloes were studied. isolation of the pathogen was attempted from the samples by selective enrichment in university of vermont medium and plating onto dominguez-rodriguez isolation agar. pathogenicity of the isolates was tested by christie, atkins, munch petersen test and mice incoulation test. listeria spp. and l. monocytogenes were i ... | 2004 | 14690723 |
| gadd45beta is important for perpetuating cognate and inflammatory signals in t cells. | gadd45beta (growth arrest and dna damage-inducible, beta) is involved in cell cycle arrest, apoptosis, signal transduction and cell survival. in t cells, gadd45b was rapidly induced by t cell receptor (tcr) and inflammatory signals. deficiency of gadd45beta in cd4+ t cells impaired their responses to tcr stimulation or inflammatory cytokines. erk, p38 and jnk activation were all substantially suppressed in gadd45beta-deficient cd4+ t cells. cytokine production by gadd45beta-deficient cd4+ t cell ... | 2004 | 14691480 |
| macrophage activation and fcgamma receptor-mediated signaling do not require expression of the slp-76 and slp-65 adaptors. | the src-homology 2 domain-containing, leukocyte-specific phosphoprotein of 76 kda (slp-76) is a hematopoietic adaptor that plays a central role during immunoreceptor-mediated activation of t lymphocytes and mast cells and collagen receptor-induced activation of platelets. despite similar levels of expression in macrophages, slp-76 is not required for fc receptor for immunoglobulin g (igg; fcgammar)-mediated activation. we hypothesized that the related adaptor slp-65, which is also expressed in m ... | 2004 | 14694181 |
| inhibition of listeria monocytogenes by enterocin ej97 produced by enterococcus faecalis ej97. | enterocin ej97 from enterococcus faecalis ej97 showed a concentration-dependent antimicrobial activity against listeria monocytogenes cect 4032. activity of enterocin ej97 against l. monocytogenes cect 4032 increased slightly at 4 degrees c, and cold-adapted cells did not show any increased resistance. sensitivity of l. monocytogenes cect 4032 to enterocin ej97 was not modified by the addition of sodium benzoate, sodium acetate, nacl or sodium tripolyphosphate. anti-listeria activity was enhance ... | 2004 | 14698097 |
| evaluation of a challenge testing protocol to assess the stability of ready-to-eat cooked meat products against growth of listeria monocytogenes. | challenge testing of ready-to-eat (rte) foods with listeria monocytogenes is recommended to assess the potential for growth. the present study was undertaken to evaluate a protocol for challenge testing applied to rte cooked meat products. in order to choose l. monocytogenes strains with a representative behaviour, initially, the variability of the response of multiple l. monocytogenes strains of human and food origin to different stress and growth conditions was established. the strains were no ... | 2004 | 14698103 |
| evidence implicating the 5' untranslated region of listeria monocytogenes acta in the regulation of bacterial actin-based motility. | the acta protein of listeria monocytogenes is a major virulence factor, essential for the recruitment and polymerization of host actin filaments that lead to intracellular motility and cell-to-cell spread of bacteria within the infected host. the expression of acta is tightly regulated and is strongly induced only when l. monocytogenes is within the host cytosol. intracellular induction of acta expression is mediated through a single promoter element that directs the expression of a messenger rn ... | 2004 | 14706101 |
| mice deficient in lrg-47 display increased susceptibility to mycobacterial infection associated with the induction of lymphopenia. | although ifn-gamma is essential for host control of mycobacterial infection, the mechanisms by which the cytokine restricts pathogen growth are only partially understood. lrg-47 is an ifn-inducible gtp-binding protein previously shown to be required for ifn-gamma-dependent host resistance to acute listeria monocytogenes and toxoplasma gondii infections. to examine the role of lrg-47 in control of mycobacterial infection, lrg-47(-/-) and wild-type mice were infected with mycobacterium avium, and ... | 2004 | 14707092 |
| pathology of listerial encephalitis in chickens in japan. | neural signs (torticollis, drowsiness) and mortality were observed in five chickens of a native chicken flock (reared for meat) that included 450 male birds on a farm that had 2300 native chickens and 1120 layers. histologic lesions were observed in the medulla oblongata, optic lobe, cerebellum, and spinal cord of the affected birds. the lesions, which were most severe in the medulla oblongata, were massive abscesses with rarefaction (demyelination and malacia) of the parenchyma with gram-positi ... | 2003 | 14709003 |
| high affinity, paralog-specific recognition of the mena evh1 domain by a miniature protein. | many protein domains involved in cell signaling contain or interact with proline-rich sequences, and the design of molecules that perturb signaling pathways represents a foremost goal of chemical biology. previously we described a protein design strategy in which the well-folded alpha-helix in avian pancreatic polypeptide (app) presents short alpha-helical recognition epitopes. the miniature proteins designed in this way recognize even shallow protein clefts with high affinity and specificity. h ... | 2004 | 14709031 |
| generation and utilization of polyclonal antibodies to a synthetic c-terminal amino acid fragment of divercin v41, a class iia bacteriocin. | polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized c-terminal part of divercin v41 (dvnct) conjugated to the carrier protein keyhole limpet hemocyanin (klh). the sensitivity and reactivity of the dvnct-klh-generated antibodies were evaluated by enzyme-linked immunosorbent assay (elisa) using supernatant from cultures of 13 representative lactic acid bacterium strains, and specificity was confirmed by western blot analysis. anti-dvnct-klh antibodies ... | 2004 | 14711648 |
| the antimicrobial activity of extracts of the lichen cetraria aculeata and its protolichesterinic acid constituent. | in this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen cetraria aculeata has been investigated. the extracts were tested against twelve bacteria and eight fungi and found active against escherichia coli, staphylococcus aureus, aeromonas hydrophila, proteus vulgaris, streptococcus faecalis, bacillus cereus, bacillus subtilis, pseudomonas aeruginosa, listeria monocytogenes. no antimicrobial activity against the fungi was detected. it was determin ... | 2003 | 14713164 |
| comparative analysis of multilocus sequence typing and pulsed-field gel electrophoresis for characterizing listeria monocytogenes strains isolated from environmental and clinical sources. | one hundred seventy-five listeria monocytogenes strains were characterized by serotyping, pulsed-field gel electrophoresis (pfge), and multilocus sequence typing (mlst) based on loci in acta, betl, hlya, gyrb, pgm, and reca. one hundred twenty-two sequence types (sts) were identified by mlst based on allelic profiles of the four housekeeping genes (betl, gyrb, pgm, and reca), and 34 and 38 alleles were identified for hlya and acta, respectively. several acta and hlya alleles appeared to be predo ... | 2004 | 14715765 |
| effect of reheating on viability of a five-strain mixture of listeria monocytogenes in vacuum-sealed packages of frankfurters following refrigerated or frozen storage. | the purpose of this study was to assess consumer preferences for storing and reheating frankfurters and to use this information to assess the effect of product formulation and storage times and temperatures on the viability of listeria monocytogenes after reheating of frankfurters. individual links were inoculated with about 8.0 log cfu per package of a five-strain mixture of the pathogen, vacuum sealed, and stored at 4 degrees c for 3 and 15 days and at -18 degrees c for 30 days. frankfurters f ... | 2004 | 14717354 |
| reduction and survival of listeria monocytogenes in ready-to-eat meats after irradiation. | a five-strain listeria monocytogenes culture was inoculated onto six different types of ready-to-eat (rte) meats (frankfurters, ham, roast beef, bologna, smoked turkey with lactate, and smoked turkey without lactate). the meats were vacuum packed and stored at 4 degrees c for 24 h prior to irradiation. populations of l. monocytogenes were recovered by surface plating on nonselective and selective media. the margins of safety studied include 3-log (3d) and 5-log (5d) reduction of pathogenic bacte ... | 2004 | 14717355 |
| antimicrobial and antioxidant activities of natural extracts in vitro and in ground beef. | inhibition of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes by grape seed extract (activin) and pine bark extract (pycnogenol) and the effect of these natural extracts on the oxidative stability of raw ground beef were studied. in an agar dilution test, the mics of activin and pycnogenol were determined to be 4.0 mg/ml for 4.43 log cfu per plate of e. coli o157:h7 and 4.0 mg/ml for 4.38 log cfu per plate of l. monocytogenes. in an inhibition curve test, populations ... | 2004 | 14717365 |
| bacterial contamination of recirculating brine used in the commercial production of moisture-enhanced pork. | in a commercial process for the production of moisture-enhanced pork, boneless pork loins were conveyed through a recirculating injection apparatus, and brine (sodium phosphate, sodium chloride, and lemon juice solids) was pumped into the meat through banks of needles inserted automatically into the upper surfaces of cuts. brine samples were collected at intervals during the production process and analyzed to determine the total plate count and the numbers of lactic acid bacteria, pseudomonads, ... | 2004 | 14717372 |
| enrichment and dna extraction protocols for the simultaneous detection of salmonella and listeria monocytogenes in raw sausage meat with multiplex real-time pcr. | a novel method of dna extraction and purification was developed and was used in conjunction with a multiplex real-time pcr assay for the simultaneous detection of salmonella and listeria monocytogenes in a raw meat sample. the pcr used primers targeting the inva gene of salmonella and the hlya gene of l. monocytogenes, and pcr products were detected with a lightcycler on the basis of fluorescence from sybr green and melting temperature. the assay allowed the detection of 3 listeria cells and 4 s ... | 2004 | 14717373 |
| effect of prestorage treatmlents and storage conditions on the survival of salmonella enteritidis pt4 and listeria monocytogenes on fresh marine and freshwater aquaaculture fish. | the effect of prestorage treatments, such as immersion in a sorbate solution (5%, wt/vol), heating (60 degrees c, 1 min), and a combination of the two treatments, and the subsequent storage in air or under modified atmosphere packaging (map; 40% co2, 30% o2, and 30% n2) at chill temperatures (0 +/- 1 degrees c), on listeria monocytogenes and salmonella enteritidis pt4 was studied. the prestorage treatments affected the pathogenic bacteria, and in all cases, there was a decrease in their populati ... | 2004 | 14717374 |
| [the importance of a complete diagnostic workup in patients with nontraumatic (partial) paraplegia]. | in two women, aged 86 and 56 years, respectively, who suffered from back pain and loss of strength, and in a 55-year-old man who lost sensation and strength in his left leg, spinal-cord compression in connection with vertebral destruction was seen on radiological examination. when spinal-cord compression is the result of a local malignant tumour, the therapy often entails emergency radiotherapy. in the first two patients, histological examination revealed a solitary plasmocytoma and curative hig ... | 2003 | 14723022 |
| effects of high-pressure processing on listeria monocytogenes, spoilage microflora and multiple compound quality indices in chilled cold-smoked salmon. | to evaluate the effect of high-pressure processing (hpp) on listeria monocytogenes, microbial and chemical changes and shelf-life in chilled cold-smoked salmon (css). | 2004 | 14723701 |
| regulation of transcription of compatible solute transporters by the general stress sigma factor, sigmab, in listeria monocytogenes. | listeria monocytogenes is well known for its durable physiological characteristics, which allow the organism to grow at low temperature and ph and high osmolarity. growth under high osmolarity depends on the accumulation of compatible solutes, among which glycine betaine and carnitine are the preferred solutes for this organism. three different transport systems, gbu, betl, and opuc, have been identified in l. monocytogenes which serve to scavenge the preferred compatible solutes. the general st ... | 2004 | 14729706 |
| lactoferricin influences early events of listeria monocytogenes infection in thp-1 human macrophages. | bovine lactoferrin (blf) and its derivative peptide lactoferricin b (lfcinb) are known for their antimicrobial activity towards several pathogens, including listeria monocytogenes, a food-borne gram-positive invasive bacterium that infects a wide variety of host cells, including professional phagocytes. to add further information on the antibacterial effects of these compounds, the influence of blf, lfcinb and the antimicrobial centre of lfcinb, the hexapeptide lfcinb(4-9), on the invasive behav ... | 2004 | 14729926 |
| global changes in gene expression observed at the transition from growth to stationary phase in listeria monocytogenes scotta batch culture. | listeria monocytogenes is a food-borne gram-positive bacterium that is responsible for a variety of infections (worldwide) annually. the organism is able to survive a variety of environmental conditions and stresses, however, the mechanisms by which l. monocytogenes adapts to environmental change are yet to be fully elucidated. an understanding of the mechanism(s) by which l. monocytogenes survives unfavourable environmental conditions will aid in developing new food processing methods to contro ... | 2004 | 14730677 |
| prosthetic valve endocarditis due to listeria monocytogenes. report of two cases and reviews. | endocarditis due to listeria monocytogenes is a rare but serious disease often leading to valve dysfunction and heart failure. two cases of listerial prosthetic valve endocarditis are reviewed along with 66 cases previously reported. | 2004 | 14732327 |
| regression of hpv-positive tumors treated with a new listeria monocytogenes vaccine. | human papillomavirus (hpv) has been implicated in the pathogenesis of 15% to 23% of head and neck squamous cell carcinomas as well as most oropharyngeal carcinomas. the viral oncoproteins e6 and e7 are expressed in hpv-positive tumor cells and therefore provide ideal targets for tumor immunotherapy. because of its unique ability to induce a cellular immune response, the intracellular bacteria listeria monocytogenes has been studied as a potential hpv-positive tumor vaccine. | 2004 | 14732776 |
| roles of caspase-1 in listeria infection in mice. | caspase-1 [il-1beta-converting enzyme (ice)] processes substrate precursor molecules to yield the biologically active form of il-1beta and il-18, both of which are considered to play important roles in the host defense by activation of both innate and adaptive immunity. we evaluated the immune response of caspase-1(-/-) mice to listeria monocytogenes (lm) infection. lm eradication in the early phase of infection was impaired in the mutant mice with a prominent decrease in il-18 and ifn-gamma pro ... | 2004 | 14734619 |
| escherichia coli expressing recombinant antigen and listeriolysin o stimulate class i-restricted cd8+ t cells following uptake by human apc. | vaccination against cancer or intracellular pathogens requires stimulation of class i-restricted cd8(+) t cells. it is therefore important to develop ag delivery vectors that will promote cross-presentation by apcs and stimulate appropriate inflammatory responses. toward this goal, we tested the potential of escherichia coli as an ag delivery vector in in vitro human culture. bacteria expressing enhanced green fluorescent protein were internalized efficiently by dendritic cells, as shown by flow ... | 2004 | 14734740 |
| antigen-specific cd8+ t cell responses in intestinal tissues during murine listeriosis. | infection of mice with listeria monocytogenes induces a strong cd8+ t cell response, which is critical for the control of bacteria and for protection against re-infection. we analyzed the cd8+ t cell response in different intestinal tissues following oral and intravenous (i.v.) l. monocytogenes infection. after oral infection, bacterial titers in small intestine and large intestine, and the listeria-specific cd8+ t cell response in the mucosa of both parts of the intestine, were highly correlate ... | 2004 | 14738888 |
| adhesins and invasins of pathogenic bacteria: a structural view. | adhesion and invasion of pathogenic bacteria represent the important initial step of infection. pathogens utilize surface-located adhesins/invasins for specific interaction with host cell receptors. the three-dimensional structures of a number of adhesins/invasins show that many are elongated molecules containing domains commonly found in eukaryotic proteins. similar folds are employed repeatedly to target different receptors. | 2004 | 14738899 |
| absence of interferon-gamma-inducible gene igtp does not significantly alter the development of chagasic cardiomyopathy in mice infected with trypanosoma cruzi (brazil strain). | interferon-gamma (ifn-gamma) contributes to host resistance during acute infection with trypanosoma cruzi, the causative agent of chagas' disease. inducibly expressed guanosine triphosphatase (igtp), a 48-kda guanosine triphosphatase (gtpase), is a member of a family of gtpase proteins inducibly expressed by ifn-gamma. the expression pattern of igtp suggests that it may mediate ifn-gamma-induced responses in a variety of cell types. igtp has been demonstrated to be important for control of toxop ... | 2003 | 14740917 |
| disruption of putative regulatory loci in listeria monocytogenes demonstrates a significant role for fur and perr in virulence. | the ability to adapt to adverse environmental conditions encountered in food and during host infection is a sine qua non for a successful listeria monocytogenes infection. this ability is likely to depend on complex regulatory pathways controlled by a number of key regulators. we utilized the pori19 plasmid integration system to analyze the role of six putative regulatory loci in growth under suboptimal environmental conditions and during murine infection. disruption of loci encoding a topoisome ... | 2004 | 14742513 |
| heat shock protein 60 acts as a receptor for the listeria adhesion protein in caco-2 cells. | the 104-kda listeria adhesion protein (lap) in listeria monocytogenes is involved in binding to various mammalian cell lines. however, the receptor that interacts with lap in eukaryotic cells is unknown. in this study, scanning immunoelectron microscopy qualitatively demonstrated greater binding capacity of wild-type (wt) l. monocytogenes strain (f4244) than a lap-deficient mutant strain (kb208) to caco-2 cells. the goal of this study was identification of the host cell receptor for lap. using a ... | 2004 | 14742538 |
| chemokine receptor 5 is dispensable for innate and adaptive immune responses to listeria monocytogenes infection. | chemokine receptor 5 (ccr5) binds macrophage inflammatory protein 1alpha (mip-1alpha), mip-1beta, rantes, and members of the monocyte chemotactic protein family and is also a receptor for human immunodeficiency virus (hiv). ccr5 ligands can suppress hiv-1 entry into cells. in humans, homozygous mutations of the ccr5 gene confer resistance to hiv-1 infection. the role of ccr5 in defense against microbial infection is unclear. in this study we examined the innate and adaptive immune responses of c ... | 2004 | 14742553 |
| new aspects regarding evolution and virulence of listeria monocytogenes revealed by comparative genomics and dna arrays. | listeria monocytogenes is a food-borne bacterial pathogen that causes a wide spectrum of diseases, such as meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. among the 13 l. monocytogenes serovars described, invasive disease is mostly associated with serovar 4b strains. to investigate the genetic diversity of l. monocytogenes strains with different virulence potentials, we partially sequenced an epidemic serovar 4b strain and compared it with the complete sequence of t ... | 2004 | 14742555 |
| listeria meningitis associated with infliximab. | to report a case of listeria monocytogenes meningitis in a 73-year-old man receiving infliximab for rheumatoid arthritis. | 2004 | 14742795 |
| mhc class ia-restricted memory t cells inhibit expansion of a nonprotective mhc class ib (h2-m3)-restricted memory response. | listeria monocytogenes infection generates major histocompatibility complex (mhc) class ia-restricted and mhc class ib-(h2-m3)-restricted effector and memory cd8+ t cells. however, only mhc class ia-restricted memory cells expand after rechallenge, and it is unknown if mhc class ib-restricted memory cd8+ t cells generated by vaccination are protective. we show here that h2-m3-restricted memory cd8+ t cells were capable of secondary expansion but, in contrast to primary h2-m3-restricted effector ... | 2004 | 14745446 |
| inefficient replication of listeria innocua in the cytosol of mammalian cells. | the efficiency of adherence to, internalization by, and replication in the cytosol of j774 macrophages and hep-2 epithelial cells was compared between a nonspreading listeria monocytogenes acta mutant and l. innocua. the studied l. innocua strains were equipped either with listeriolysin alone or with listeriolysin o (llo) and the recently identified hexose-phosphate transporter of l. monocytogenes. all listerial strains expressed green fluorescent protein (gfp) under the control of the prfa-depe ... | 2004 | 14745696 |
| study of two bacteriocins produced by enterococcus casseliflavus and ent. faecalis. | the antimicrobial activity of two plasmid-borne bacteriocins produced by enterococcus casseliflavus im 416k1 and ent. faecalis im 388c and their mating transferability were studied. | 2004 | 14746539 |
| listeria monocytogenes contamination of finishing pigs: an exploratory epidemiological survey in france. | listeria monocytogenes is a foodborne pathogen of major concern for public health in industrialised countries. since l. monocytogenes carriage by pigs at the herd level could be a primary source for carcass contamination, control measures should be designed to reduce the l. monocytogenes load at the pre-harvest stage. for this purpose, an exploratory analytical survey was carried out in 2000-2001 in 93 french farrow-to-finish pig farms concerning l. monocytogenes contamination in pigs before the ... | 2003 | 14746769 |
| treatment of experimental sepsis-induced immunoparalysis with tnf. | following a severe septic abdominal infection induced by sublethal cecal ligation and puncture (clp) in mice, a phase of depressed immune reactivity occurred two days after clp characterized by a reduced capacity to produce tnf. to determine whether this reduced tnf production causes immunoparalysis as determined by increased susceptibility to bacterial infection and whether therapeutic tnf substitution can be beneficial during this phase, a super-infection with salmonella enterica serovar typhi ... | 2003 | 14748511 |
| increased salt- and nisin-sensitivity of pressure-injured bioluminescent listeria monocytogenes. | suspensions of a bioluminescent (luxab) transformant of listeria monocytogenes in ph 7.0 phosphate buffer were pressurised and the effect of the pressure treatment was monitored by plate counting. when the bacteria were suspended in nacl- and nisin-free buffer the number of colony forming units (cfu) decreased by 3 and 6 log cycles after 300 mpa for 10 and 30 min, respectively. supplementing the plating medium with 5% nacl did not influence the colony forming capacity of non-pressurised cells, h ... | 2003 | 14750435 |
| characterization of listeria monocytogenes and listeria innocua from a vegetable processing plant by rapd and rea. | the incidence of listeria monocytogenes in a vegetable processing plant was investigated over a 23-month period. frozen ready-to-eat vegetable samples, well as the plant environment, were sampled. the molecular subtyping techniques, random amplified polymorphic dna (rapd) and restriction endonuclease analyses (rea), were performed to help investigate the origin and routes of listeria dissemination. the low and sporadic incidence of l. monocytogenes made it impossible to establish an epidemiologi ... | 2004 | 14751689 |
| occurrence of listeria monocytogenes in fresh and processed foods in navarra (spain). | the presence of listeria spp. was investigated in a total of 3685 food samples obtained from different industries and markets of northern spain in the last 4 years. the samples analyzed include fresh raw products (meat, milk and poultry) and treated products (cooked and cured meats, frozen vegetables and smoked salmon). occurrence of listeria spp. varied from 8.1% in soft cheese to 76.3% in raw poultry samples. the highest incidence of l. monocytogenes also occurred in raw poultry (36.1% positiv ... | 2004 | 14751690 |
| caat-box, contigs-assembly and annotation tool-box for genome sequencing projects. | contigs-assembly and annotation tool-box (caat-box) is a software package developed for the computational part of a genome project where the sequence is obtained by a shotgun strategy. caat-box contains new tools to predict links between contigs by using similarity searches with other whole genome sequences. most importantly, it allows annotation of a genome to commence during the finishing phase using a gene-oriented strategy. for this purpose, caat-box creates an individual protein file (ipf) ... | 2004 | 14752000 |
| [meningitis by listeria in children]. | listeria monocytogenes is a rare cause of bacterial meningoencephalitis in the non-immunocompromised pediatric population. on occasions, the absence of differential characteristics with other bacteria that cause meningitis delays diagnosis and hence treatment, worsening the prognosis. | 2004 | 14752714 |
| [listeriosis in patients with human immunodeficiency virus infection in spain. three new cases and literature review]. | listeriosis has been considered infrequent among patients infected with the human immunodeficiency virus (hiv). only a limited number of listeriosis cases among hiv-infected patients have been published in spain, where the prevalence of hiv infection is relatively high. we present our experience in this field and provide a review of the reported cases in our country. | 2004 | 14757003 |
| [listeria monocytogenes meningitis in an immunocompetent girl]. | 2004 | 14757026 | |
| signs of spinal cord disease in two heifers caused by listeria monocytogenes. | 2004 | 14758832 | |
| recurrent listeria monocytogenes aortic graft infection: confirmation of relapse by molecular subtyping. | based on molecular typing methods, we identified a rare case of a recurrent l. monocytogenes infection resulting from an infected aortic prosthesis as detected by 18-f-fluoro-d-deoxyglucose positron emission tomography (fdg pet). our case highlights the usefulness of molecular typing and nuclear imaging methods for understanding l. monocytogenes pathogenesis and epidemiology. | 2004 | 14761724 |
| clpb, a novel member of the listeria monocytogenes ctsr regulon, is involved in virulence but not in general stress tolerance. | clp-hsp100 atpases are a widespread family of ubiquitous proteins that occur in both prokaryotes and eukaryotes and play important roles in the folding of newly synthesized proteins and refolding of aggregated proteins. they have also been shown to participate in the virulence of several pathogens, including listeria monocytogenes. here, we describe a member of the clp-hsp100 family of l. monocytogenes that harbors all the characteristics of the clpb subclass, which is absent in the closely rela ... | 2004 | 14762012 |
| characterization of flagellin expression and its role in listeria monocytogenes infection and immunity. | flagellin is the structural component of flagella produced by many pathogenic bacteria and is a potent proinflammatory molecule that mediates these effects through toll-like receptor (tlr) 5. in listeria monocytogenes (lm), flagellin expression is regulated by temperature and has been described as being shut off at 37 degrees c. in this study, we demonstrate that tlr5-mediated cell activation and flagellin expression is maintained at 37 degrees c in some laboratory-adapted strains and in approxi ... | 2004 | 14764107 |
| cutting edge: toll-like receptor signaling in macrophages induces ligands for the nkg2d receptor. | macrophages recognize the presence of infection by using the toll-like receptor (tlr) family of proteins that detect ligands on bacterial, viral, and fungal pathogens. we show that murine macrophages stimulated with pathogen products known to signal through tlrs express ligands for the nkg2d receptor, found on nk cells, activated cd8(+) t cells and activated macrophages. tlr signaling, through the myd88 adaptor, up-regulates transcription of the retinoic acid early inducible-1 (rae-1) family of ... | 2004 | 14764662 |
| extracellular replication of listeria monocytogenes in the murine gall bladder. | the bacterium listeria monocytogenes can cause a life-threatening systemic illness in humans. despite decades of progress in animal models of listeriosis, much remains unknown about the processes of infection and colonization. here, we report that l. monocytogenes can replicate in the murine gall bladder and provide evidence that its replication there is extracellular and intraluminal. in vivo bioluminescence imaging was employed to determine the location of the infection over time in live anima ... | 2004 | 14764883 |
| a portable array biosensor for detecting multiple analytes in complex samples. | the multi-analyte array biosensor (maab) has been developed at the naval research laboratory (nrl) with the goal of simultaneously detecting and identifying multiple target agents in complex samples with minimal user manipulation. this paper will focus on recent improvements in the biochemical and engineering aspects of this instrument. these improvements have enabled the expansion of the repertoire of analytes detected to include salmonella typhimurium and listeria monocytogenes, and also expan ... | 2004 | 14765282 |
| multi-virulence-locus sequence typing of listeria monocytogenes. | a multi-virulence-locus sequence typing (mvlst) scheme was developed for subtyping listeria monocytogenes, and the results obtained using this scheme were compared to those of pulsed-field gel electrophoresis (pfge) and the published results of other typing methods, including ribotyping (rt) and multilocus sequence typing (mlst). a set of 28 strains (eight different serotypes and three known genetic lineages) of l. monocytogenes was selected from a strain collection (n > 1,000 strains) to repres ... | 2004 | 14766571 |
| development and validation of experimental protocols for use of cardinal models for prediction of microorganism growth in food products. | an experimental protocol to validate secondary-model application to foods was suggested. escherichia coli, listeria monocytogenes, bacillus cereus, clostridium perfringens, and salmonella were observed in various food categories, such as meat, dairy, egg, or seafood products. the secondary model validated in this study was based on the gamma concept, in which the environmental factors temperature, ph, and water activity (aw) were introduced as individual terms with microbe-dependent parameters, ... | 2004 | 14766591 |
| deregulation of listeria monocytogenes virulence gene expression by two distinct and semi-independent pathways. | expression of the major virulence cluster in listeria monocytogenes is positively regulated by the transcription factor prfa and is influenced by several environmental factors, including the presence of readily metabolized carbohydrates such as cellobiose and glucose. although little is understood about the mechanisms through which environmental factors influence expression of the prfa regulon, evidence for structural and functional similarities of prfa to the crp-fnr family of regulatory protei ... | 2004 | 14766910 |
| physiological implications of class iia bacteriocin resistance in listeria monocytogenes strains. | high-level resistance to class iia bacteriocins has been directly associated with the absent eiiab(man) (mpta) subunit of the mannose-specific phosphoenolpyruvate-dependent phosphotransferase system (pts) (eiit(man)) in listeria monocytogenes strains. class iia bacteriocin-resistant strains used in this study were a spontaneous resistant, l. monocytogenes b73-mr1, and a defined mutant, l. monocytogenes egde-mpta. both strains were previously reported to have the eiiab(man) pts component missing. ... | 2004 | 14766911 |
| the cd8 population in cd4-deficient mice is heavily contaminated with mhc class ii-restricted t cells. | in experiments to study the impact of deficiency in cd4+ t cell help on the magnitude of cd8+ cytotoxic t cell response to pathogens, it was noted that in cd4 gene knockout mice, the cd8 population made significant responses to several nominally major histocompatibility complex (mhc) class ii-restricted epitopes in addition to the expected responses to mhc class i-restricted epitopes. a similar response by cd8+ t cells to class ii-restricted epitopes was not observed in wild-type mice, or in mic ... | 2004 | 14769854 |
| a report on the isolation of listeria (listerella) organisms from a canary and a chicken in the province of alberta. | 1950 | 14778039 | |
| listeria monocytogenes associated with bovine abortion. | 1951 | 14803381 | |
| listeria monocytogenes as the cause of disease in man and animals, and its relation to infectious mononucleosis from an etiological and immunological aspect. | 1951 | 14810715 | |
| a four-year study of listeriosis in michigan. | 1951 | 14824018 | |
| effect of aureomycin and chloramphenicol on two recently isolated strains of listeria monocytogenes. | 1951 | 14844232 | |
| listeriosis; field and laboratory studies, and aureomycin activity. | 1951 | 14847112 | |
| [morphology, culture and experimental pathogenicity of corynebacterium infantisepticum]. | 1951 | 14856244 | |
| marinococcus halophilus dsm 20408t encodes two transporters for compatible solutes belonging to the betaine-carnitine-choline transporter family: identification and characterization of ectoine transporter ectm and glycine betaine transporter betm. | in response to osmotic stress, the halophilic, gram-positive bacterium marinococcus halophilus accumulates compatible solutes either by de novo synthesis or by uptake from the medium. to characterize transport systems responsible for the uptake of compatible solutes, a plasmid-encoded gene bank of m. halophilus was transferred into the transport-deficient strain escherichia coli mkh13, and two genes were cloned by functional complementation required for ectoine and glycine betaine transport. the ... | 2004 | 14872322 |
| meningitis due to listeria monocytogenes; report of two cases. | 1952 | 14888480 | |
| a comparison of the traditional method of counting viable cells and a quick microplate method for monitoring the growth characteristics of listeria monocytogenes. | to determine: (i) the growth parameters (specific growth rate, lag time, asymptotic amount of growth, generation time and time for maximum growth rate) of listeria monocytogenes in different broths by standard cultivation methods and (ii) whether a microplate method in conjunction with a standard nondedicated plate reader could be adapted to routine assay. | 2004 | 14962037 |
| microbiological examination of ready-to-eat cold sliced meats and pâté from catering and retail premises in the uk. | to establish the microbiological quality of cold ready-to-eat sliced meats and pâté from catering and retail premises, and investigate links hypothesized between foodborne campylobacter infection and the consumption of cold sliced meats. | 2004 | 14962130 |
| left thalamo-peduncular abscess caused by listeria monocytogenes in a homeless patient. | 2003 | 14962707 | |
| [microbiological study of the meals served in school lunch rooms on the island of tenerife, spain]. | school lunchrooms and catered meals are of major importance from the public health standpoint. this study is aimed at evaluating the microbiological quality of the meals served in school lunchrooms for the purpose of ascertaining whether it is suitable or, to the contrary, the intake thereof may involve a serious health problem for this high-risk group. | 2003 | 14965066 |
| [a case report of listeria monocytogenes infection in a patient with aids. efficacy of treatment with cotrimoxazole associated with rhug-csf (filgrastim)]. | listeriosis is an emerging opportunistic infection in the immunocompromised host. a case of sepsis due to listeria monocytogenes in a patient with advanced hiv infection and severe neutropenia, treated for an underlying non-hodgkin's lymphoma, is described. therapy with cotrimoxazole associated with rhug-csf (filgrastim) led to a rapidly favourable clinical and microbiological outcome, and to the correction of concurrent neutropenia. the case report is discussed according to a literature review ... | 1997 | 14966399 |