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arsenite oxidizing multiple metal resistant bacteria isolated from industrial effluent: their potential use in wastewater treatment.arsenite oxidizing bacteria, isolated from industrial wastewater, showed high resistance against arsenite (40 mm) and other heavy metals (10 mm pb; 8 mm cd; 6 mm cr; 10 mm cu and 26.6 mm as(5+)). bacterial isolates were characterized, on the basis of morphological, biochemical and 16s rrna ribotyping, as bacillus cereus (1.1s) and acinetobacter junii (1.3s). the optimum temperature and ph for the growth of both strains were found to be 37 °c and 7. both the strains showed maximum growth after 24 ...201627339314
microbiological isolates and antibiotic susceptibilities: a 10-year review of culture-proven endophthalmitis cases.to review the microbiological spectrum and antibiotic sensitivities of the pathogens that cause culture-proven endophthalmitis and to understand the status and trends of antibiotic susceptibility at a public hospital over a 10-year period.201727347700
chemical profile, antioxidant and antibacterial activities of achillea moschata wulfen, an endemic species from the alps.aerial parts of achillea moschata wulfen (asteraceae) growing wild in the italian rhaetian alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. inspection of the metabolic profile combining hplc-dad and esi-ms/ms data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. among them, the major compound was 7-o-glucosyl apigenin. caffeoyl derivates were other phen ...201627347915
photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity.we present the simple, eco-friendly synthesis of silver nanoparticles (agnps) using sunlight or green, red, blue, or white led light together with dryopteris crassirhizoma rhizome extract (dcre) as the reducing and capping agent. the preliminary indication of agnp production was a color change from yellowish green to brown after light exposure in the presence of dcre. optimization of parameters such as ph, inoculum dose, and metal ion concentration played an important role in achieving nanoparti ...201627348063
modulation of phytoremediation and plant growth by the treatment with pgpr, ag nanoparticle and untreated municipal wastewater.the present attempt was made to determine the effects of untreated municipal wastewater (mw) on growth and physiology of maize and to evaluate the role of ag nanoparticle and plant-growth-promoting rhizobacteria (pgpr) when interacting with mw used for irrigation. it was used for the isolation of pgpr. the isolates were identified and characterized based on the colony morphology, c/n source utilization pattern using miniaturized identification system (qts 24), catalase (cat) and oxidase tests, a ...201627348506
isolation, partial purification and evaluation of bioactive compounds from leaves of ageratum houstonianum.the present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of ageratum houstonianum (asteraceae). the quantification and the identification of compounds in the crude extract and active bands isolated by preparative tlc were accomplished using gc-ms analysis. the most important compounds identified in the crude extract and active bands (ab-1 and ab-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and ...201227350770
identification of pbc218/pbc210 genes of bacillus cereus g9241 in five florida soils using qpcr.the distribution of the virulent plasmid pbc210 of b. cereus that carries several b. anthracis genes and has been implicated in lethal anthrax-like pulmonary disease is unknown. we screened our collection of 103 b. cereus isolates and 256 soil samples using a quantitative pcr (qpcr) assay that targeted three open reading frames putatively unique to pbc210. when tested with dna from 2 b. cereus strains carrying pbc210, and 64 gram-positive and 55 gram-negative bacterial species, the assay had 100 ...201427350995
genetic and functional analyses of krs, a locus encoding kurstakin, a lipopeptide produced by bacillus thuringiensis.bacteria of the bacillus genus are able to synthesize several families of lipopeptides. these small molecules are the product of non-ribosomal peptide synthetases. in 2000, it was found that bacillus thuringiensis, an entomopathogenic bacterium of the bacillus cereus group, produced a previously unknown lipopeptide: kurstakin. genomic analyses reveal that the krs locus, encoding the kurstakin synthetases, is specific to the b. cereus group, but is unevenly distributed within this group. previous ...201627353188
effect of endophytic bacillus cereus erbp inoculation into non-native host: potentials and challenges for airborne formaldehyde removal.phytoremediation could be a cost-effective, environmentally friendly approach for the treatment of indoor air. however, some drawbacks still dispute the expediency of phytotechnology. our objectives were to investigate the competency of plant growth-promoting (pgp) endophytic bacillus cereus erbp (endophyte root blue pea), isolated from the root of clitoria ternatea, to colonize and stabilize within zamioculcas zamiifolia and euphorbia milii as non-native hosts without causing any disease or str ...201627362296
combined effect of anaerobiosis, low ph and cold temperatures on the growth capacities of psychrotrophic bacillus cereus.psychrotrophic strains of the foodborne pathogen bacillus cereus can multiply during the refrigerated storage of food products. the aim of this study was to determine the impact of anaerobiosis on the growth of two psychrotrophic b. cereus strains exposed to acidic ph at a cold temperature in a laboratory medium. at 10 °c, growth occurred at ph values equal to or higher than 5.7 during anaerobiosis, whereas aerobic growth was observed from ph 5.4. growth rates during aerobiosis were similar at p ...201627375252
comparative genomics of iron-transporting systems in bacillus cereus strains and impact of iron sources on growth and biofilm formation.iron is an important element for bacterial viability, however it is not readily available in most environments. we studied the ability of 20 undomesticated food isolates of bacillus cereus and two reference strains for capacity to use different (complex) iron sources for growth and biofilm formation. studies were performed in media containing the iron scavenger 2,2-bipyridine. transcriptome analysis using b. cereus atcc 10987 indeed showed upregulation of predicted iron transporters in the prese ...201627375568
common mechanism of cross-resistance development in pathogenic bacteria bacillus cereus against alamethicin and pediocin involves alteration in lipid composition.to understand the mechanism of development of cross-resistance in food pathogen bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followed by cation exchange chromatography with 14.01-fold purity and 14.4 % recovery. b. cereus strains alamethicin-resistant strains (ic50 3.23 µg/ml) were selected from sensitive popula ...201627378130
molecular characteristics of lysozyme g in euryglossa orientalis; cdna cloning, phylogenic analysis, physicochemical properties and tissue gene expression.lysozymes are the key molecules of innate immune system against bacterial infections. in the present study, we identified the molecular characteristics, physicochemical properties, antibacterial activity, evolutionary relationship and tissue expression pattern of g-type lysozyme in euryglossa orientalis (euor lysg). the full-length euor lysg cdna is composed of 588 nucleotides and an open reading frame encoding a protein with 195 amino acids with more than 65 % identity to g-type lysozyme of sol ...201627393386
low-cost and eco-friendly green synthesis of silver nanoparticles using prunus japonica (rosaceae) leaf extract and their antibacterial, antioxidant properties.low cost and eco-friendly green synthesis of silver nanoparticles (agnps) from silver nitrate (agno3) using prunus japonica leaves extract as reducing agent by a simple method at room temperature. the biosynthesized nanoparticles (nps) were characterized by uv-vis, tunneling electron microscopy (hr-tem), scanning electron microscopy (sem) coupled with x-ray energy dispersive spectrophotometer (edax), x-ray diffraction (xrd) and fourier transform infrared spectroscopy (ftir). in uv-vis spectrosco ...201627396523
antimicrobial and anti-quorum sensing activities of selected medicinal plants of ethiopia: implication for development of potent antimicrobial agents.traditional medicinal plants have been used as an alternative medicine in many parts of the world, including ethiopia. there are many documented scientific reports on antimicrobial activities of the same. to our knowledge, however, there is no report on the anti-quorum sensing (quorum quenching, qq) potential of traditional ethiopian medicinal plants. as many of the opportunistic pathogenic bacteria depend on quorum sensing (qs) systems to coordinate their virulence expression, interference with ...201627400878
discovery of a unique extracellular polysaccharide in members of the pathogenic bacillus that can co-form with spores.an exopolysaccharide, produced during the late stage of stationary growth phase, was discovered and purified from the culture medium of bacillus cereus, bacillus anthracis, and bacillus thuringiensis when strains were grown in a defined nutrient medium that induces biofilm. two-dimensional nmr structural characterization of the polysaccharide, named pzx, revealed that it is composed of an unusual three amino-sugar sequence repeat of [-3)xylnac4oac(α1-3)glcnaca4oac(α1-3)xylnac(α1-]n the sugar res ...201627402849
integrative approach for producing hydrogen and polyhydroxyalkanoate from mixed wastes of biological origin.in this study, an integrative approach to produce biohydrogen (h2) and polyhydroxyalkanoates (pha) from the wastes of biological origin was investigated. a defined set of mixed cultures was used for hydrolysis and the hydrolysates were used to produce h2. the effluent from h2 production stage was used for pha production. under batch culture, a maximum of 62 l h2/kg of pure potato peels (total solid, ts 2 %, w/v) and 54 l h2/kg of mixed biowastes (mbw1) was recorded. using effluent from the h2 pr ...201627407293
bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico.currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ...201627407294
[functional analysis of grp and iris, the gag and env domesticated errantivirus genes, in the drosophila melanogaster genome].drosophila melanogaster is the only invertebrate that contains endogenous retroviruses, which are called errantiviruses. two domesticated genes, grp and iris, which originate from errantivirus gag and env, respectively, have been found in the d. melanogaster genome. the functions performed by the genes in drosophila are still unclear. to identify the functions of domesticated gag and env in the d. melanogaster genome, expression of iris and grp was studied in strains differing by the presence or ...201727414781
complete genome sequences of nine bacillus cereus group phages.we report the sequences of nine novel bacillus cereus group bacteriophages: dignkc, juglone, nemo, nigalana, notthecreek, phrodo, sagefayge, vinny, and zuko. these bacteriophages are double-stranded dna-containing myoviridae isolated from soil samples using b. thuringiensis subsp. kurstaki as the host bacterium.201627417827
bacillus spore wet heat resistance and evidence for the role of an expanded osmoregulatory spore cortex.previous work reported that decoated bacillus cereus spores incubated in 4 mol l(-1) cacl2 are killed at lower temperatures than spores in water. this wet heat sensitization was suggested to support a role for an osmoregulatory peptidoglycan cortex in spore cores' low water content, and their wet heat resistance. current work has replicated this finding with spores of b. cereus, bacillus megaterium and bacillus subtilis. however, this work found that decoated spores apparently killed at 80°c in ...201627424522
evolutionary processes and environmental factors underlying the genetic diversity and lifestyles of bacillus cereus group bacteria.bacteria of the bacillus cereus group are environmental gram-positive spore-forming bacteria ubiquitously distributed. despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychrophylic to thermotolerant strains. most of the phenotypes are linked to the presence of large plasmids that encode for diverse toxins. however, other processes, like mutation or reco ...201627424810
evaluating bionanoparticle infused fungal metabolites as a novel antimicrobial agent.therapeutic properties of fungal metabolites and silver nanoparticles have been well documented. while fungal metabolites have been used for centuries as medicinal drugs, potential of biogenic silver nanoparticles has recently received attention. we have evaluated the antimicrobial potential of aspergillus terreus crude extract, silver nanoparticles and an amalgamation of both against four pathogenic bacterial strains. antimicrobial activity of the following was evaluated - a. terreus extract, b ...201727429931
bacillus cereus group-type strain-specific diagnostic peptides.the bacillus cereus group consists of eight very closely related species and comprises both harmless and human pathogenic species such as bacillus anthracis, bacillus cereus, and bacillus cytotoxicus. numerous efforts have been undertaken to allow presumptive differentiation of b. cereus group species from one another. however, methods to rapidly and accurately distinguish these species are currently lacking. we confirmed that classical matrix-assisted laser desorption/ionization-time-of-flight ...201627432653
intravenous drug abuse by patients inside the hospital: a cause for sustained bacteremia.patients with history of intravenous drug abuse are noted to be at risk of several infections including hiv, endocarditis, and other opportunistic infections. we report the case of a patient with sustained bacillus cereus bacteremia despite use of multiple antibiotic regimens during his inpatient stay. our case highlights the importance of high suspicion for active drug use inside the hospital in such patients. this is important in order to minimize unnecessary diagnostic workup and provide adeq ...201627433362
the bacterial dna repair protein mfd confers resistance to the host nitrogen immune response.production of reactive nitrogen species (no) is a key step in the immune response following infections. no induces lesions to bacterial dna, thus limiting bacterial growth within hosts. using two pathogenic bacteria, bacillus cereus and shigella flexneri, we show that the dna-repair protein mfd (mutation-frequency-decline) is required for bacterial resistance to the host-no-response. in both species, a mutant deficient for mfd does not survive to no, produced in vitro or by phagocytic cells. in ...201627435260
expression of the genes encoding the cask/r two-component system and the desa desaturase during bacillus cereus cold adaptation.two-component systems (tcs) allow a cell to elaborate a variety of adaptive responses to environment changes. the recently discovered cask/r tcs plays a role in the optimal unsaturation of fatty acids necessary for cold adaptation of the foodborne-pathogen bacillus cereus here, we showed that the promoter activity of the operon encoding this tcs was repressed during growth at low temperature in the stationary phase in the parental strain when compared to the cask/r mutant, suggesting that casr n ...201627435329
the comer gene plays an important role in biofilm formation and sporulation in both bacillus subtilis and bacillus cereus.bacteria adopt alternative cell fates during development. in bacillus subtilis, the transition from planktonic growth to biofilm formation and sporulation is controlled by a complex regulatory circuit, in which the most important event is activation of spo0a, a transcription factor and a master regulator for genes involved in both biofilm formation and sporulation. in b. cereus, the regulatory pathway controlling biofilm formation and cell differentiation is much less clear. in this study, we sh ...201627446060
chemical composition and biological activity of centaurea baseri: new species from turkey.the genus centaurea l. is one of the largest and important genera of asteraceae family. centaurea species have been widely used as herbal remedies in folk medicine for their antidandruff, antidiarrheic, antirheumatic, anti-inflammatory, choleretic, diuretic, digestive, stomachic, astringent, antipyretic, cytotoxic, and antibacterial properties. centaurea baseri kose & alan is a recently described local endemic species in turkey and this is the first study on the chemical composition and bioactiv ...201627447348
chemical variability and biological activities of essential oils of micromeria inodora (desf.) benth. from algeria.the chemical composition of the essential oils isolated from the aerial parts of micromeria inodora (desf.) benth. collected in 24 algerian localities was investigated from the first time using gc-fid, gc/ms and (13) c-nmr. altogether, 83 components which accounted for 94.7% of the total oil composition were identified. the main compounds were trans-sesquisabinene hydrate (1; 20.9%), α-terpinyl acetate (2; 19.8%), globulol (3; 4.9%), caryophyllene oxide (4; 4.3%), β-bisabolol (5; 2.9%) and trans ...201627448034
microbial diversity and biochemical characteristics of borassus akeassii wine.palm wine produced traditionally and consumed by many people in the south-west of burkina faso is subject to alteration. in this study, we carried out a follow-up of two palm wines' fermentation during the 10 days in which palm wines are classically produced and consumed. we monitored biochemical characteristics of fermenting wines as well as followed the microflora kinetics using culture-dependent and culture-independent methods. the analysis of the acid content and the bacterial population rev ...201627450560
bacillus cereus biofilms-same, only different.bacillus cereus displays a high diversity of lifestyles and ecological niches and include beneficial as well as pathogenic strains. these strains are widespread in the environment, are found on inert as well as on living surfaces and contaminate persistently the production lines of the food industry. biofilms are suspected to play a key role in this ubiquitous distribution and in this persistency. indeed, b. cereus produces a variety of biofilms which differ in their architecture and mechanism o ...201627458448
lactobacillus delbrueckii subsp lactis cidca 133 modulates response of human epithelial and dendritic cells infected with bacillus cereus.it is known that probiotic microorganisms are able to modulate pathogen virulence. this ability is strain dependent and involves multiple interactions between microorganisms and relevant host's cell populations. in the present work we focus on the effect of a potentially probiotic lactobacillus strain (lactobacillus delbrueckii subsp. lactis cidca 133) in an in vitro model of bacillus cereus infection. our results showed that infection of intestinal epithelial ht-29 cells by b. cereus induces nu ...201627459335
prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death.the antimicrobial activity of prodigiosin from serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens bacillus cereus, staphylococcus aureus, pseudomonas aeruginosa and escherichia coli. the mode of action of prodigiosin was studied. prodigiosin induced bactericidal activity indicating a stereotypical set of biochemical and morphological feature of programmed cell death (pcd). pcd involves dna fragmentation, generation of ros, and expression of a ...201627460563
characterization of antimicrobial substance from lactobacillus salivarius kl-d4 and its application as biopreservative for creamy filling.lactobacillus salivarius kl-d4 isolated from duck intestine produced bacteriocin which was stable at high temperature and a wide ph range of 3-10. its cell free supernatant at ph 5.5 exhibited wide inhibitory spectrum against both g+ and g- bacteria. the highest bacteriocin production was obtained in mrs broth supplemented with 0.5 % (w/v) caco3 at 6 h by gentle shaking. pcr walking using specific primers at the conserved region of class-ii bacteriocin resulted in 4 known genes of kld1, kld2, kl ...201627462508
enhanced efficacy of clindamycin hydrochloride encapsulated in pla/plga based nanoparticle system for oral delivery.clindamycin hydrochloride (clh) is a clinically important oral antibiotic with wide spectrum of antimicrobial activity that includes gram-positive aerobes (staphylococci, streptococci etc.), most anaerobic bacteria, chlamydia and certain protozoa. the current study was focused to develop a stabilised clindamycin encapsulated poly lactic acid (pla)/poly (d,l-lactide-co-glycolide) (plga) nano-formulation with better drug bioavailability at molecular level. various nanoparticle (nps) formulations o ...201627463797
changes in microbial composition and the prevalence of foodborne pathogens in crab marinated in soy sauce produced by six manufacturing plants.the present study examined the changes in microbiological composition during the production process of crab marinated in soy sauce, potential microbial hazards, potential contamination routes and effective critical control points. crab and soy sauce samples were obtained from six different manufacturing plants at different stages, and their microbiological content was comprehensively assessed by quantitative and qualitative analyses.201727469473
development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites.the intrinsic biodegradability of hydrocarbons and the distribution of proficient degrading microorganisms in the environment are very crucial for the implementation of bioremediation practices. among others, one of the most favorable methods that can enhance the effectiveness of bioremediation of hydrocarbon-contaminated environment is the application of biosurfactant producing microbes. in the present study, the biodegradation capacities of native bacterial consortia toward total petroleum hyd ...201627471499
hexane extract of echinops spinosissimus turra subsp. spinosus from tunisia: a potential source of acetylated sterols - investigation of its biological activities.the hexane extract of echinops spinosissimus turra subsp. spinosus flower heads was analyzed for its fatty acid and sterol composition. its physicochemical characteristics were also studied. the saponification, iodine and peroxide values were determined as 255 mg koh/g, 42.57 g i2 /100 g and 110 m equiv. o2 /kg of oil, respectively. the oleic (c18:1; 61.14%), palmitic (c16:0; 21.36%) and linoleic (c18:2; 10.45%) acids were the dominant fatty acids. this extract was also found to contain high lev ...201627476999
biocidal and sporicidal efficacy of pathoster(®) 0.35% and pathoster(®) 0.50% against bacterial agents in potential bioterrorism use.the use of products that can neutralize or significantly reduce the microbial load and that are not harmful to human health and the environment represents a milestone in the fight against the spread of infectious diseases. peracetic acid, besides being an excellent sterilizing and sporicidal agent, is harmless to humans and the environment when it is used in a common dosage. however, the high costs and loss of efficacy of the product very quickly after its reconstitution limit its use. we evalua ...201627482880
recovery of heat treated bacillus cereus spores is affected by matrix composition and factors with putative functions in damage repair.the ability of spores to recover and grow out after food processing is affected by cellular factors and by the outgrowth conditions. in the current communication we studied the recovery and outgrowth of individually sorted spores in bhi and rice broth media and on agar plates using flow cytometry. we show that recovery of wet heat treated bacillus cereus atcc 14579 spores is affected by matrix composition with highest recovery in bhi broth or on rice agar plates, compared to bhi agar plates and ...201627486443
thusin, a novel two-component lantibiotic with potent antimicrobial activity against several gram-positive pathogens.due to the rapidly increasing prevalence of multidrug-resistant bacterial strains, the need for new antimicrobial drugs to treat infections has become urgent. bacteriocins, which are antimicrobial peptides of bacterial origin, are considered potential alternatives to conventional antibiotics and have attracted widespread attention in recent years. among these bacteriocins, lantibiotics, especially two-component lantibiotics, exhibit potent antimicrobial activity against some clinically relevant ...201627486447
design, synthesis, anticancer, antimicrobial activities and molecular docking studies of theophylline containing acetylenes and theophylline containing 1,2,3-triazoles with variant nucleoside derivatives.a new series of theophylline containing acetylene derivatives (6a-6b and 7-13) and theophylline containing 1,2,3-triazoles with variant nucleoside derivatives (20-32) have been designed and synthesized. these compounds were screened for anticancer and antimicrobial activity. further the computational docking and 2d qsar were performed using moe software to identify novel scaffolds. the results showed that compound 29 and 30 exhibit significant cytotoxic effect on all four cancer cells such as lu ...201627487568
the roles of atxa orthologs in virulence of anthrax-like bacillus cereus g9241.atxa is a critical transcriptional regulator of plasmid-encoded virulence genes in bacillus anthracis. bacillus cereus g9241, which caused an anthrax-like infection, has two virulence plasmids, pbcxo1 and pbc210, that each harbor toxin genes and a capsule locus. g9241 also produces two orthologs of atxa: atxa1, encoded on pbcxo1, and atxa2, encoded on pbc210. the amino acid sequence of atxa1 is identical to that of atxa from b. anthracis, while the sequences of atxa1 and atxa2 are 79% identical ...201627490458
simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods.capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ...201627494415
comparative evaluation of cyanide removal by adsorption, biodegradation, and simultaneous adsorption and biodegradation (sab) process using bacillus cereus and almond shell.the present study aimed to investigate the removal efficiency of cyanide from contaminated water by adsorption, biodegradation and simultaneous adsorption and biodegradation (sab) process individually in a batch reactor. adsorption was achieved by using almond shell granules and biodegradation was conducted with suspended cultures of bacillus cereus, whereas sab process was carried out using bacillus cereus and almond shell in a batch reactor. the effect of agitation time, ph, and initial cyanid ...201627498500
degradation of acephate by enterobacter asburiae, bacillus cereus and pantoea agglomerans isolated from diamondback moth plutella xylostella (l), a pest of cruciferous crops.acephate-degrading bacterial isolates were isolated from the larval gut of diamondback moth plutella xylostella, a notorious pest of cruciferous crops worldwide that has developed resistance to insecticides. partial 16s rrna gene sequencing identified the isolates as bacillus cereus (px-b.c.or), enterobacter asburiae (pxe), and pantoae agglomerans (px-pt.ag.jor). all isolates grew on minimal media (mm) in the presence of acephate at 100 and 200 ppm, with maximum growth at 200 ppm. lc-ms analyses ...201627498509
use of ferrous iron by metallo-β-lactamases.metallo-β-lactamases (mbls) catalyse the hydrolysis of almost all β-lactam antibacterials including the latest generation carbapenems and are a growing worldwide clinical problem. it is proposed that mbls employ one or two zinc ion cofactors in vivo. isolated mbls are reported to use transition metal ions other than zinc, including copper, cadmium and manganese, with iron ions being a notable exception. we report kinetic and biophysical studies with the di-iron(ii)-substituted metallo-β-lactamas ...201627498591
production of hemolysin bl by bacillus cereus group isolates of dairy origin is associated with whole-genome phylogenetic clade.bacillus cereus group isolates that produce diarrheal or emetic toxins are frequently isolated from raw milk and, in spore form, can survive pasteurization. several species within the b. cereus group are closely related and cannot be reliably differentiated by established taxonomical criteria. while b. cereus is traditionally recognized as the principal causative agent of foodborne disease in this group, there is a need to better understand the distribution and expression of different toxin and ...201627507015
evaluation of health potential of nutritionally enriched kodo millet (eleusine coracana) grown in himachal pradesh, india.in this study, kodo millet grains were phytochemically investigated for their nutritional and antioxidant potential for their use as functional foods. methanolic extracts of grains showed higher phenolic content and antioxidant activity. tlc studies of the extracted polyphenols from kodo millet showed the predominant presence of ferulic acid and cinnamic acid in the millet. further quantification of these polyphenols was done by using hplc, analysing ferulic acid and cinnamic acid. antagonistic ...201727507461
potential risk of acute toxicity induced by agi cloud seeding on soil and freshwater biota.silver iodide is one of the most common nucleating materials used in cloud seeding. previous cloud seeding studies have concluded that agi is not practically bioavailable in the environment but instead remains in soils and sediments such that the free ag amounts are likely too low to induce a toxicological effect. however, none of these studies has considered the continued use of this practice on the same geographical areas and thus the potential cumulative effect of environmental agi. the aim o ...201627517140
antimicrobial activity of various cationic molecules on foodborne pathogens.antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, gram-positive and gram-negative bacteria. the minimum inhibitory concentration (mic) and the minimum bactericidal concentration (mbc) of α-poly-l-lysine (poly-lys), α-poly-l-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on bacillus subtilis, ...200727517822
bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic bacillus cereus group species isolated from dairy foods and dairy environments.a facultatively anaerobic, spore-forming bacillus strain, fsl w8-0169t, collected from raw milk stored in a silo at a dairy powder processing plant in the north-eastern usa was initially identified as a bacillus cereus group species based on a partial sequence of the rpob gene and 16s rrna gene sequence. analysis of core genome single nucleotide polymorphisms clustered this strain separately from known b. cereus group species. pairwise average nucleotide identity blast values obtained for fsl w8 ...201627520992
mechanism of enhanced antibacterial activity of ultra-fine zno in phosphate buffer solution with various organic acids.ultra-fine-zno showed low toxicity in complex water matrix containing multiple components such as pbs buffer and the toxic mechanism of ultra-fine-zno has not been clearly elucidated. in present study, enhanced antibacterial activity of 200 nm diameter ultra-fine-zno in pbs buffer against bacillus cereus and escherichia coli were observed in the presence of several organic acids in comparison with ultra-fine-zno in pbs buffer alone. these findings indicated that the toxic effects of the ultra-fi ...201627524254
persistent bacillus cereus bacteremia in 3 persons who inject drugs, san diego, california, usa.bacillus cereus is typically considered a blood culture contaminant; however, its presence in blood cultures can indicate true bacteremia. we report 4 episodes of b. cereus bacteremia in 3 persons who inject drugs. multilocus sequence typing showed that the temporally associated infections were caused by unrelated clones.201627533890
new insights into the interaction between the quorum-sensing receptor nprr and its dna target, or the response regulator spo0f.the nprr protein and nprrb signaling peptide comprise a bifunctional quorum-sensing system from the bacillus cereus group that is involved in transcriptional activation through dna-binding and in sporulation initiation by binding to spo0f. we characterized in vitro the direct interactions established by nprr that may be relevant for performing its two functions. apo-nprr interacted with spo0f, but not with the target dna. the nprrb signaling peptide sskpdivg that binds strongly to apo-nprr, fail ...201627543719
proteome data to explore the impact of pbclin15 on bacillus cereus atcc 14579.this data article reports changes in the cellular and exoproteome of b. cereus cured from pbclin15.time-course changes of proteins were assessed by high-throughput nanolc-ms/ms. we report all the peptides and proteins identified and quantified in b. cereus with and without pbclin15. proteins were classified into functional groups using the information available in the kegg classification and we reported their abundance in term of normalized spectral abundance factor. the repertoire of experiment ...201627547804
myrcia ovata cambessedes essential oils: a proposal for a novel natural antimicrobial against foodborne bacteria.this paper reports the innovative antibacterial activity of essential oils (eos) from nine myrcia ovata cambessedes plants against eight foodborne bacteria. staphylococcus aureus, bacillus cereus, bacillus subtilis, enterococcus faecalis and pseudomonas aeruginosa were the most susceptible bacteria to eos. in particular, the p. aeruginosa, which is usually resistant to antimicrobials agents, was extremely sensitive to some eos. the gram-positive and gram-negative bacteria were inhibited and elim ...201627554278
investigation on antimicrobial agents of the terrestrial streptomyces sp. bcc71188.the terrestrial actinomycete strain bcc71188 was identified as streptomyces by its morphology (having spiral chain spore on the aerial mycelium), chemotaxonomy (containing ll-diaminopimelic acid in the cell wall), and 16s rrna gene sequence analysis [showing high similarity values compared with streptomyces samsunensis m1463(t) (99.85 %) and streptomyces malaysiensis nbrc 16446(t) (99.40 %)]. the crude extract exhibited antimalarial against plasmodium falciparum (ic50 0.19 μg/ml), anti-tb agains ...201727554496
activation of the class ib ribonucleotide reductase by a flavodoxin reductase in bacillus cereus.to reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class ib ribonucleotide reductase (rnr) must be activated via a pathway that involves redox protein(s). the reduced flavoprotein nrdi is an important protein in this pathway, as it reduces dioxygen to superoxide. superoxide then reacts with the rnr mn(ii)2 site to generate a tyrosyl radical that is required for catalysis. a native nrdi reductase has not yet been identified. we herein demonstrate through kinetic and sp ...201627559930
a catalytic role for c-h/π interactions in base excision repair by bacillus cereus dna glycosylase alkd.dna glycosylases protect genomic integrity by locating and excising aberrant nucleobases. substrate recognition and excision usually take place in an extrahelical conformation, which is often stabilized by π-stacking interactions between the lesion nucleobase and aromatic side chains in the glycosylase active site. bacillus cereus alkd is the only dna glycosylase known to catalyze base excision without extruding the damaged nucleotide from the dna helix. instead of contacting the nucleobase itse ...201627571247
teaching neuroimages: diffuse cerebral vasospasm and multiple intracranial abscesses from bacillus cereus. 201627572436
identification of pyridine synthase recognition sequences allows a modular solid-phase route to thiopeptide variants.thiopeptides are structurally complex, bioactive natural products derived from ribosomally synthesized and post-translationally modified peptides. a remarkable set of enzymes were recently revealed to catalyze the formation of the core trithiazolylpyridine of thiopeptides via a formal [4 + 2] cycloaddition. these pyridine synthases typically act late in thiopeptide biosynthesis to affect macrocyclization and cleavage of the n-terminal leader peptide, making them potentially useful biocatalysts f ...201627575591
the pesticidal cry6aa toxin from bacillus thuringiensis is structurally similar to hlye-family alpha pore-forming toxins.the cry6 family of proteins from bacillus thuringiensis represents a group of powerful toxins with great potential for use in the control of coleopteran insects and of nematode parasites of importance to agriculture. these proteins are unrelated to other insecticidal toxins at the level of their primary sequences and the structure and function of these proteins has been poorly studied to date. this has inhibited our understanding of these toxins and their mode of action, along with our ability t ...201627576487
factors of detection of bacillus cereus strains in eye cream.bacillus cereus has been associated with clinical infections and is also the cause of post-traumatic endophthalmitis as well as endogenous eye infections, which can result in blindness. cosmetics, although preserved, can be contaminated during manufacture or use and thus cause serious health issues.201727578165
identification and analysis of informative single nucleotide polymorphisms in 16s rrna gene sequences of the bacillus cereus group.analysis of 16s rrna genes is important for phylogenetic classification of known and novel bacterial genera and species and for detection of uncultivable bacteria. pcr amplification of 16s rrna genes with universal primers produces a mixture of amplicons from all rrna operons in the genome, and the sequence data generally yield a consensus sequence. here we describe valuable data that are missing from consensus sequences, variable effects on sequence data generated from nonidentical 16s rrna amp ...201627582514
bacterial succession and metabolite changes during flax (linum usitatissimum l.) retting with bacillus cereus hdym-02.high-throughput sequencing and gc-ms (gas chromatography-mass spectrometry) were jointly used to reveal the bacterial succession and metabolite changes during flax (linum usitatissimum l.) retting. the inoculation of bacillus cereus hdym-02 decreased bacterial richness and diversity. this inoculum led to the replacement of enterobacteriaceae by bacillaceae. the level of aerobic pseudomonadaceae (mainly azotobacter) and anaerobic clostridiaceae_1 gradually increased and decreased, respectively. f ...201627585559
draft genome sequence of biocontrol agent bacillus cereus uw85.bacillus cereus uw85 was isolated from a root of a field-grown alfalfa plant from arlington, wi, and identified for its ability to suppress damping off, a disease caused by phytophthora megasperma f. sp. medicaginis on alfalfa. here, we report the draft genome sequence of b. cereus uw85, obtained by a combination of sanger and illumina sequencing.201627587823
cloning, purification and characterization of the collagenase cola expressed by bacillus cereus atcc 14579.bacterial collagenases differ considerably in their structure and functions. the collagenases colh and colg from clostridium histolyticum and cola expressed by clostridium perfringens are well-characterized collagenases that cleave triple-helical collagen, which were therefore termed as ´true´ collagenases. cola from bacillus cereus (b. cereus) has been added to the collection of true collagenases. however, the molecular characteristics of b. cereus cola are less understood. in this study, we id ...201627588686
partial characterization of bacteriocin-like compounds from two strains of bacillus cereus with biological activity against paenibacillus larvae, the causal agent of american foulbrood disease.american foulbrood (afb), caused by the spore-forming gram-positive bacterium paenibacillus larvae, is the most severe bacterial disease affecting honeybees worldwide. two bacterial isolates showing specific inhibitory activity against p. larvae were identified as bacillus cereus by 16s rdna sequencing. antagonistic compounds were obtained from cell-free supernatants of strains m6c and m387 growing on trypticase soy broth and concentrated by nh4 so4 precipitation, ultrafiltration and butanol ext ...201627589675
genotoxicity evaluation of tannery effluent treated with newly isolated hexavalent chromium reducing bacillus cereus.in this study, the efficiency of free and immobilized cells of newly isolated hexavalent chromium [cr(vi)] reducing bacillus cereus strain cr1 (accession no. kj162160) was studied in the treatment of tannery effluent. the analysis of effluents revealed high chemical oxygen demand (cod-1260 mg/l), biological oxygen demand (bod5-660 mg/l), total dissolved solids (tds-14000 mg/l), electrical conductivity (ec-21.5 ms/cm) and total chromium (tc-2.4 mg/l). the effluents also showed genotoxic effects t ...201627591849
the comparative analysis of antioxidant and biological activity for the dendropanax morbifera lev. leaves extracted by different ethanol concentrations.the different concentrations of ethanol (20-100%) and distilled water extract for dendropanax morbifera lev. leaves were evaluated to induce antioxidant and biological activity employed by variety of assays. the 20%, 80%, and 100% ethanol extract demonstrated the relatively higher activity, whereas distilled water, 40%, and 60% ethanol extracts exhibited the lower antioxidant and biological activity. especially, 80% ethanol extract showed the remarkably higher radical scavenging activity, reduci ...201627592831
the structure of the pleiotropic transcription regulator cody provides insight into its gtp-sensing mechanism.gtp and branched-chain amino acids (bcaas) are metabolic sensors that are indispensable for the determination of the metabolic status of cells. however, their molecular sensing mechanism remains unclear. cody is a unique global transcription regulator that recognizes gtp and bcaas as specific signals and affects expression of more than 100 genes associated with metabolism. herein, we report the first crystal structures of the full-length cody complex with sensing molecules and describe their fun ...201627596595
pereskia aculeata muller (cactaceae) leaves: chemical composition and biological activities.the aims of this work were to study the chemical composition of the essential oil from the leaves of pereskia aculeata and to evaluate some biological activities of three leaf extracts. the phenolic content, antioxidant activity, and in vitro antimicrobial and antifungal activities were determined. the methanol extract showed antioxidant activity (ec50 7.09 mg/ml) and high polyphenols content (15.04 ± 0.31 mg gallic acid equivalents (gae)/g). the petroleum ether extract exhibited potent antibact ...201627598154
termitarium-inhabiting bacillus endophyticus tsh42 and bacillus cereus tsh77 colonizing curcuma longa l.: isolation, characterization, and evaluation of their biocontrol and plant-growth-promoting activities.bacillus strains were isolated from termitarium soil and screened for their antifungal activity through the production of diffusible and volatile metabolites. further, the bacterial strains that showed antifungal activity were evaluated for their biocontrol potential on the basis of their plant-growth-promoting attributes. termitarium-inhabiting bacillus strains tsh42 and tsh77 significantly reduced the growth of pathogenic fungus fusarium solani, controlled the symptoms of rhizome rot in turmer ...201627604298
bacillus cereus biovar anthracis causing anthrax in sub-saharan africa-chromosomal monophyly and broad geographic distribution.through full genome analyses of four atypical bacillus cereus isolates, designated b. cereus biovar anthracis, we describe a distinct clade within the b. cereus group that presents with anthrax-like disease, carrying virulence plasmids similar to those of classic bacillus anthracis. we have isolated members of this clade from different mammals (wild chimpanzees, gorillas, an elephant and goats) in west and central africa (côte d'ivoire, cameroon, central african republic and democratic republic ...201627607836
antimicrobial and antioxidant activity of chitosan/hydroxypropyl methylcellulose film-forming hydrosols hydrolyzed by cellulase.the aim of this study was to evaluate the impact of cellulase (c) on the biological activity of chitosan/hydroxypropyl methylcellulose (ch/hpmc) film-forming hydrosols. the hydrolytic activity of cellulase in two concentrations (0.05% and 0.1%) was verified by determination of the progress of polysaccharide hydrolysis, based on viscosity measurement and reducing sugar-ends assay. the 2,2-diphenyl-1-picrylhydrazyl (dpph) free radical scavenging effect, the ferric reducing antioxidant power (frap) ...201627608008
itraq-based proteomic analysis of li-f type peptides produced by paenibacillus polymyxa jsa-9 mode of action against bacillus cereus.li-f type peptides (amp-jsa9) produced by paenibacillus polymyxa jsa-9 are a group of cyclic lipodepsipeptide antibiotics that exhibit a broad antimicrobial spectrum against gram-positive bacteria and filamentous fungi, especially bacillus cereus and fusarium moniliforme. in this study, to better understand the antibacterial mechanism of amp-jsa9 against b. cereus, the ultrastructure of amp-jsa9-treated b. cereus cells was observed by both atomic force microscopy and transmission electron micros ...201727609309
differentiation of foodborne bacteria using nir hyperspectral imaging and multivariate data analysis.the potential for near-infrared (nir) hyperspectral imaging and multivariate data analysis to be used as a rapid non-destructive tool for detection and differentiation of bacteria was investigated. nir hyperspectral images were collected of bacillus cereus, escherichia coli, salmonella enteritidis, staphylococcus aureus and staphylococcus epidermidis grown on agar for 20 h at 37 °c. principal component analysis (pca) was applied to mean-centred data. standard normal variate (snv) correction and ...201627624097
possession, use, and transfer of select agents and toxins-- addition of bacillus cereus biovar anthracis to the hhs list of select agents and toxins. interim final rule and request for comments.the centers for disease control and prevention (cdc) in the department of health and human services (hhs) is adding bacillus cereus biovar anthracis to the list of hhs select agents and toxins as a tier 1 select agent. we are taking this action to regulate this agent that is similar to b. anthracis to prevent its misuse, which could cause a biological threat to public health and/or national security.201627632805
chemical composition and in vitro evaluation of antimicrobial, antioxidant and antigerminative properties of the seed oil from the tunisian endemic ferula tunetana pomel ex batt.essential oil of the seeds from the endemic tunisian plant ferula tunetana pomel ex batt. was analyzed for its chemical composition and screened for its antimicrobial, antioxidant and antigerminative properties. the chemical composition of the isolated oil is reported for the first time. according to the gc/fid, gc/ms and (13) c-nmr analysis results; 18 compounds were identified accounting for 84.6% of the total oil. the chemical composition of this essential oil was characterized by the presenc ...201727638478
phenolic compounds and in vitro antibacterial and antioxidant activities of three tropic fruits: persimmon, guava, and sweetsop.in our previous study, we have found that persimmon, guava, and sweetsop owned considerably high antioxidant activity and contained high total phenolic contents as well. in order to further supply information on the antibacterial and antioxidant activity of these three tropic fruits, they were extracted by 80% methanol. we then examined the extractions about their phenolic compounds and also studied the extractions and phenolic contents about their minimum inhibitory concentration (mic) and mini ...201627648444
pellino-1 derived cationic antimicrobial prawn peptide: bactericidal activity, toxicity and mode of action.the antimicrobial peptides (amps) are multifunctional molecules which represent significant roles in the innate immune system. these molecules have been well known for decades because of their role as natural antibiotics in both invertebrates and vertebrates. the development of multiple drug resistance against conventional antibiotics brought a greater focus on amps in recent years. the cationic peptides, in particular, proven as host defense peptides and are considered as effectors of innate im ...201627648859
chemical composition, antibacterial and phytotoxic activities of peganum harmala seed essential oils from five different localities in northern africa.peganum harmala l., also known as syrian rue or pègano, is a herbaceous plant belonging to the zygohpyllaceae family, and is widely used in traditional medicine. the chemical composition of essential oils of p. harmala seeds from five different regions of northern africa (algeria, egypt, libya, morocco and tunisia) was studied by gc and gc-ms analyses. a total of 105 compounds were identified, the main components being oxygenated monoterpenes and oxygenated sesquiterpenes. eugenol is the main co ...201627649128
chemical composition and in vitro antimicrobial, cytotoxic, and central nervous system activities of the essential oils of citrus medica l. cv. 'liscia' and c. medica cv. 'rugosa' cultivated in southern italy.citrus medica cv. 'liscia' and c. medica cv. 'rugosa' are two taxa of citron, belonging to the biodiversity of south italy, in particular of amalfi coast, in the campania region. the chemical composition of the essential oils (eos) from fruit peels of both c. medica cultivars was studied by gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) analyses. in all, 100 compounds were identified, 82 for c. medica cv. 'liscia', accounting for 91.4% of the total oil, and 88 for c. me ...201627649138
commercial disinfectants during disinfection process validation: more failures than success.disinfection process validation is mandatory before introduction of a new disinfectant in hospital services. commercial disinfection brands often question existing hospital policy claiming greater efficacy and lack of toxicity of their products. inadvertent inadequate disinfection leads to morbidity, patient's economic burden, and the risk of mortality.201627656441
the heat resistance and spoilage potential of aerobic mesophilic and thermophilic spore forming bacteria isolated from chinese milk powders.the propensity for aerobic bacilli and allied genera to produce highly heat-resistant spores and thermally stable spoilage enzymes are major bacteriological issues faced by the dairy industry. most of the enzymes are able to survive any heat treatment applied during the manufacture of milk powders and have the potential to remain active in milk powders and other dairy products during storage, and may explain some of the sensory and functionality defects reported in dairy products. despite many r ...201627657656
bioactivity of a novel glycolipid produced by a halophilic buttiauxella sp. and improving submerged fermentation using a response surface method.an antimicrobial glycolipid biosurfactant (gbs), extracted and identified from a marine bacterium, was studied to inhibit pathogenic microorganisms. production of the gbs was optimized using a statistical method, a response surface method (rsm) with a central composite design (ccd) for obtaining maximum yields on a cost-effective substrate, molasses. the gbs-producing bacterium was identified as buttiauxella species in terms of biochemical and molecular characteristics. this compound showed a de ...201627669197
genome sequence of bacillus cereus group phage salinjah.the double-stranded dna (dsdna) myoviridae bacillus cereus group bacteriophage salinjah was isolated from soil collected in gyeonggi-do, south korea. salinjah, a cluster c phage with a broad host range, suggests the need to create a new subcluster with salinjah and helga as founding members.201627688335
draft genome sequence of bacillus cereus lcr12, a plant growth-promoting rhizobacterium isolated from a heavy metal-contaminated environment.bacillus cereus lcr12 is a plant growth-promoting rhizobacterium, isolated from a heavy metal-contaminated environment. the 6.01-mb annotated genome sequence provides the genetic basis for revealing its potential application to remediate contaminated soils in association with plants.201627688340
the comparing of antimicrobial activity of csn1s2 protein of fresh milk and yoghurt goat breed ethawah inhibited the pathogenic bacteria.goat milk is reported to have antimicrobial activity of several pathogen bacteria that contained on food materials. the research related with antimicrobial activity of alpha-s2 casein from goat milk is relatively less than other casein components. herein, we reported the antimicrobial activity of caprine alpha-s2 casein (csn1s2) protein from ethawah breed goat milk and yoghurt in gram positive (listeria monocytogenes, staphylococcus aureus and bacillus cereus) and negative pathogen bacteria (esc ...201627698594
the thnr gene is a negative transcription regulator of the thurincin h genetic cassette in bacillus thuringiensis subsp. morrisoni.thurincin h is a bacteriocin synthesized by some strains of bacillus thuringiensis. in this study, the thurincin h genetic cassette, which contains ten genes, from a mexican strain of b. thuringiensis subsp. morrisoni (btm) was cloned and sequenced. to study the function of the thnr gene component in the cassette, we generated various constructs with or without thnr for expression in btm. all transformants harboring thnr in recombinant plasmids showed a decrease of ~15 to ~90 % in inhibitory act ...201727699438
biochemical and biological characterization of two brassicaceae after their commercial expiry date.two brassicaceae (eruca sativa, brassica oleracea var. sabauda) were stored in air and under a modified atmosphere for several days after their expiry date and then analyzed. the polyphenol content and composition, as well as the antioxidant activity of the extracts, were assessed, compared to the fresh products. antimicrobial properties on tester strains (bacillus cereus, escherichia coli, staphylococcus aureus and pseudomonas aeruginosa) and in vitro anti-proliferative activity were evaluated. ...201727719918
bacillus cereus causing intratumoral brain abscess.we report a case of intratumoral brain abscess due to bacillus cereus in an adult male patient, which was managed successfully with excision of lesion and piperacillin-tazobactam for the duration of 5 weeks. to the best of our knowledge, this is a first case report of b. cereus infection leading to intratumoral brain abscess in a patient with a history of steroid administration by the intravenous route.201727721298
feasible management of southern corn leaf blight via induction of systemic resistance by bacillus cereus c1l in combination with reduced use of dithiocarbamate fungicides.dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. the objective of this study was to develop a strategy to effectively cont ...201627721698
genome sequence of bacillus cereus forc_021, a food-borne pathogen isolated from a knife at a sashimi restaurant.bacillus cereus causes food-borne illness through contaminated foods; therefore, its pathogenicity and genome sequences have been analyzed in several studies. we sequenced and analyzed b. cereus strain forc_021 isolated from a sashimi restaurant. the genome sequence consists of 5,373,294 bp with 35.36% gc contents, 5,350 predicted cdss, 42 rrna genes, and 107 trna genes. based on in silico dna-dna hybridization values, b. cereus atcc 14579(t) was closest to forc_021 among the complete genome-seq ...201627728961
genotypic heterogeneity of emetic toxin producing bacillus cereus isolates from china.emetic toxin-producing bacillus cereus (emetic b. cereus) is the third member of b. cereus group whose toxins are encoded by megaplasmids, beside anthrax and insecticidal toxins of b. anthracis and b. thuringiensis, respectively. a total of 18 emetic isolates collected from food poisoning events, clinical and non-random food samples in zhejiang province of china, were analyzed by plasmid screening, pulse field gel electrophoresis, multilocus sequence typing, and toxic gene identification to inve ...201727744366
raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets.microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ...201727747902
isolation, identification and characterization of paenibacillus polymyxa cr1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production.paenibacillus polymyxa is a plant-growth promoting rhizobacterium that could be exploited as an environmentally friendlier alternative to chemical fertilizers and pesticides. various strains have been isolated that can benefit agriculture through antimicrobial activity, nitrogen fixation, phosphate solubilization, plant hormone production, or lignocellulose degradation. however, no single strain has yet been identified in which all of these advantageous traits have been confirmed.201627756215
adaptation in bacillus cereus: from stress to disease.bacillus cereus is a food-borne pathogen that causes diarrheal disease in humans. after ingestion, b. cereus experiences in the human gastro-intestinal tract abiotic physical variables encountered in food, such as acidic ph in the stomach and changing oxygen conditions in the human intestine. b. cereus responds to environmental changing conditions (stress) by reversibly adjusting its physiology to maximize resource utilization while maintaining structural and genetic integrity by repairing and m ...201627757102
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