Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| wilms tumors: relationship of nuclear deoxyribonucleic acid ploidy to patient survival. | nuclear deoxyribonucleic acid ploidy studies with paraffin-embedded archival tumor specimens were performed by flow cytometry on extracted nuclei from 56 wilms tumors. before the era of chemotherapy 9 patients had a 33 per cent survival rate at 5 years. no significant correlation between deoxyribonucleic acid ploidy pattern and survival was seen in this early group of patients. since 1960, 47 patients underwent radical nephrectomy and received chemotherapy. deoxyribonucleic acid histograms in th ... | 2010 | 2821294 |
| [anderson-fabry disease. current status. apropos of a case]. | after submitting a typical case of anderson-fabry disease in a 38 years old male, we discuss the eminent aspects of this metabolic disorder and, above all, the clinical, biochemical and ultrastructural problems of diagnosis in the heterozygotic relatives. | 2009 | 2821332 |
| [anderson-fabry disease. current status. apropos of a case]. | after submitting a typical case of anderson-fabry disease in a 38 years old male, we discuss the eminent aspects of this metabolic disorder and, above all, the clinical, biochemical and ultrastructural problems of diagnosis in the heterozygotic relatives. | 2009 | 2821332 |
| renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c. | renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ... | 1987 | 2822007 |
| renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c. | renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ... | 1987 | 2822007 |
| cloning and expression of the salmonella enterotoxin gene. | this report examines the genetic basis for salmonella typhimurium q1 enterotoxin production. a 918-base-pair xbai-hincii fragment of plasmid pjm17, composed of cholera toxin (ct) coding sequences (ctxab), was used as a gene probe. with this probe, the s. typhimurium enterotoxin was identified on a 6.3-kilobase ecori-psti fragment of chromosomal dna from plasmidless strain q1. we cloned this 6.3-kilobase fragment into escherichia coli rr1. the genetic map of the cloned salmonella enterotoxin (stx ... | 1987 | 2822664 |
| dependence of an adenosine-activated potassium current on a gtp-binding protein in mammalian central neurons. | neurons in hippocampal and striatal cell cultures respond to adenosine with an inhibitory potassium current. this response disappears during whole-cell patch-clamp recording in which the cell is filled with minimal saline. we have found that this loss of sensitivity to adenosine can be prevented by including 100 microm gtp in the patch electrode filling solution. gdp is less effective than gtp in supporting the adenosine response, while gmp has little, if any, effect. treatments known to inhibit ... | 1987 | 2822865 |
| deferoxamine induced decreases of lipid peroxides in rheumatoid arthritis. | eleven patients with rheumatoid arthritis (ra) were treated with intraarticular deferoxamine or placebo to test the hypothesis that iron chelation decreased hydroxyl radical mediated lipid peroxidation in ra. intraarticular administration of deferoxamine 100 mg resulted in predominantly systemic effects with decreased serum ferritin and decreased serum levels of lipid peroxidation products. similar changes were not detected in synovial fluid at this dose. iron chelation with deferoxamine may pro ... | 2010 | 2822924 |
| synthesis of protein in intestinal cells exposed to cholera toxin. | the mechanism by which cyclic adenosine monophosphate (amp), formed by intestinal epithelial cells in response to cholera toxin, ultimately results in alterations in water and electrolyte transport is poorly understood. several studies have indicated that inhibitors of transcription or translation block much of the transport of ions and water in the intestine and edema formation in tissue elicited by cholera toxin. data presented in this study confirmed the inhibitory effects of cycloheximide on ... | 1987 | 2823275 |
| pulmonary complications of aids: henry ford hospital experience between january 1984 and july 1986. | 1987 | 2824409 | |
| functional homology of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, phosphoglycerate mutase, and 2,3-bisphosphoglycerate mutase. | the bisphosphatase domain of the rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase has been shown to exhibit a structural similarity to yeast phosphoglycerate mutase and human red blood cell 2,3-bisphosphoglycerate mutase including very similar active site sequences with a histidyl residue being involved in phospho group transfer. the liver bifunctional enzyme was found to catalyze the hydrolysis of glycerate 1,3-bisphosphate to glycerate 3-phosphate and inorganic phosphate. the km ... | 1987 | 2824507 |
| noradrenergic mechanisms in age-related cognitive decline. | the alpha-2 agonist, clonidine, improved spatial working memory performance in 13/13 aged rhesus monkeys with documented memory impairments. the clonidine response was blocked by alpha-2, but not alpha-1 antagonists, and appeared to result from actions at postsynaptic alpha-2 receptors in that area of cortex most critical for spatial working memory, the principal sulcal cortex. the data indicate that noradrenergic mechanisms play an important role in the functioning of the frontal association co ... | 1987 | 2824692 |
| replication and packaging of choleraphage phi 149 dna. | the intercellular replication of the circularly permuted dna of choleraphage phi 149 involves a concatemeric dna structure with a size equivalent to six genome lengths. the synthesis of both monomeric and concatemeric dnas during replication of phi 149 occurred in the cytoplasm. the concatemers served as the substrate for the synthesis of mature phage dna, which was eventually packaged by a headful mechanism starting from a unique pac site in the concatemeric dna. packaging of dna into phage hea ... | 1987 | 2824829 |
| heart tumors specifically induced in young avian embryos by the v-myc oncogene. | to determine if expression of the v-myc oncogene had any effect during ontogeny, we injected avian myelocytomatosis virus strain mc29 into avian embryos at various stages of development. the injection of mc29 at embryonic day 2 (e2) or 3 (e3) caused, about 10 days later, rhabdomyosarcomas of the heart and, in some cases, skin muscle hypertrophy. when the injection was performed at e4 or e5, the number of heart tumors declined, whereas the number of skin muscle tumors increased significantly. the ... | 2009 | 2825173 |
| [bacteriologic studies of bicarbonate dialysate and suitable initial solutions]. | dialysates for the haemodialysis are produced unsterile and usually contain bacteria. own investigations of bicarbonate dialysate and adequate initial solutions comprised sterility tests, determinations of the germ count and germ tolerance experiments. only the "acid concentrate" was sterile. in the other solutions corynebacteria, acinetobacter and pseudomonas bacteria dominated as typical water germs. in the fresh reverse osmosis water and the bicarbonate dialysate as well as in the recently pr ... | 1987 | 2825447 |
| the technique of dynamic adhesive strapping for congenital clubfoot. | the use of dynamic adhesive strapping to correct congenital clubfoot in the immediate neonatal period is an effective method of treatment. the technique is described in detail, the steps are illustrated, and precautions and limitations are given. this type of treatment should shorten the amount of time required to correct congenital clubfoot. if the holding phase is sufficiently prolonged, a reduction in the number and complexity of operative procedures needed for recurrent and recalcitrant case ... | 2010 | 2825884 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex. | sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors. | 1987 | 2825912 |
| effects of caffeine and cyclic adenosine 3',5'-monophosphate on adenosine triphosphate-dependent calcium uptake by lysed brain synaptosomes. | the adenosine triphosphate-dependent calcium uptake by endoplasmic reticulum elements of lysed synaptosomes from rat brain cortex was studied. caffeine exhibited a biphasic effect on this calcium uptake activity: concentrations of 1, 2, 5, 10 or 30 mm caffeine stimulated calcium uptake by 62, 111, 73, 88 and 60% respectively, whereas calcium uptake was inhibited by 55% at a 60-mm concentration of caffeine. calcium release from endoplasmic reticulum elements of lysed brain synaptosomes was stimul ... | 1987 | 2825917 |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites. | selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ... | 1987 | 2825926 |
| murine cytomegalovirus-pseudomonas synergistic infections: comparison of virulent and attenuated virus. | the synergistic interactions between pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (mcmv) were compared in vivo. virulent mcmv challenge at a dose of 5 x 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 x 10(6) cfu/mouse of pseudomonas aeruginosa after 48 h resulted in greater than 80% mortality, apparently owing to a failure of pulmonary clearance mechanisms. single infections, or the use of attenuated mcmv in synergistic infections, did ... | 1987 | 2825950 |
| transient outward current prominent in canine ventricular epicardium but not endocardium. | previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ... | 1988 | 2826039 |
| transient outward current prominent in canine ventricular epicardium but not endocardium. | previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ... | 1988 | 2826039 |
| comparison of solutions for extracting ck, ak, and ld from brain samples. | 1987 | 2826051 | |
| tetanus toxin binds with high affinity to neuroblastoma x glioma hybrid cells ng 108-15 and impairs their stimulated acetylcholine release. | differentiated neuroblastoma x glioma hybrid cells ng 108-15 express on their surface specific binding sites for tetanus toxin. 450 sites/cell with a kd of 2 x 10(-11) m were found under "physiological" conditions of ph and salt concentrations. a hill coefficient of 1.1 indicated noncooperative binding. specific binding of 125i-toxin to its sites could be prevented either by preincubation of the toxin with a neutralizing monoclonal antibody or by pretreatment of the cells with neuraminidase (vib ... | 1987 | 2826418 |
| characterization and restriction analysis of the p sex factor and the cryptic plasmid of vibrio cholerae strain v58. | the p plasmid of vibrio cholerae is a derepressed sex factor restricted to v. cholerae and has been shown to express surface exclusion. we have isolated the plasmids of strain v58 and have found that in addition to p, two further cryptic plasmids are also present. p has a size of 68 kb as determined by both electron microscopy and restriction endonuclease analysis. these other plasmids are 34 and 4.7 kb in size. restriction maps of p and the larger cryptic plasmid have been determined. it has be ... | 1987 | 2827202 |
| [classification of spot-shaped lung changes by texture analysis]. | classification of opacities in pneumoconiosis was accomplished by textural analysis in digitised chest x-rays. a good discrimination was achieved by a set of 10 parameters. these texture measures were computed by algorithms for edge detection, local extremes, difference statistics, the co-occurrence matrix and the power spectrum. the classes of the training set were classified correctly at 99%. a test set comprising additional classes that were not contained in the training set, was classified a ... | 1987 | 2827257 |
| derivation of a monoclonal antibody against the group specific antigen of rotaviruses and its use in a diagnostic enzymatic immunoassay. | 1986 | 2827408 | |
| mucosal antibody response of calves after oral and intrabronchial administration of rotavirus. | 1987 | 2827448 | |
| herpes simplex virus infection of the hand. a profile of 79 cases. | seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ... | 1988 | 2827469 |
| pain reduction in local anesthetic administration through ph buffering. | the effects of ph buffering on the pain of administration and efficacy of three local anesthetics (1% lidocaine, 1% lidocaine with 1:100,000 epinephrine, and 1% mepivacaine) were investigated in a randomized, prospective, double-blind study of 25 adult volunteers. plain and buffered solutions of the three local anesthetics were prepared, and a 0.5 intradermal injection of each was administered. pain of anesthetic infiltration was rated from zero to ten. the area of anesthetized skin surrounding ... | 2010 | 2827545 |
| l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability. | infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ... | 1987 | 2827608 |
| l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability. | infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ... | 1987 | 2827608 |
| lipid-dependent membrane enzymes. purification to homogeneity and further characterization of diacylglycerol kinase from escherichia coli. | 1. diacylglycerol kinase apoprotein was purified from membranes of escherichia coli k12 by a six-step procedure that included hplc. the proposed assignment of the enzyme to the dgka gene [lightner et al. (1983) j. biol. chem. 258, 10856-10861] could be supported by molecular mass determination (approximately 14 kda), n-terminal sequencing (met-ala-asn), cyanogen bromide fragmentation and amino acid analysis. as predicted, proline was absent. 2. the membrane-associated as well as the butan-1-ol-d ... | 1988 | 2828054 |
| the preparation of a carbon-11 labelled neurohormone--[11c]melatonin. | melatonin, a hormone of the pineal gland, has been labelled with the positron-emitting radionuclide, carbon-11 (t1/2 = 20.4 min), for study in vivo by positron emission tomography. thus a novel labelling agent, [11c]acetyl chloride, was prepared by the carbonation of methylmagnesium bromide with cyclotron-produced [11c]carbon dioxide followed by treatment with phthaloyl dichloride. acylation of 5-methoxytryptamine then affords [11c]melatonin, which is purified by hplc and formulated for intraven ... | 2010 | 2828278 |
| a comparison of pcp-like compounds for nmda antagonism in two in vivo models. | the pcp-like compounds ketamine and dexoxadrol were evaluated in two behavioral test procedures known to be sensitive to competitive n-methyl-d-aspartate (nmda) receptor antagonists. in the nmda-induced convulsion test in mice, ketamine and dexoxadrol blocked convulsant activity only at doses that also induced nonspecific effects of pcp-like behaviors, thereby confounding the interpretation of results. these compounds also blocked nmda-induced discriminative stimuli in rats; however, this effect ... | 1988 | 2828792 |
| inhibition of rat brain adenylate cyclase activity by benzodiazepine through the effects on gi and catalytic proteins. | the effect of benzodiazepines on adenylate cyclase system was examined in rat brain. micromolar concentrations of diazepam inhibited the enzyme activity in synaptic membranes in dose- and time-dependent manners. the inhibitory effect of diazepam was more evident on the enzyme activity in the presence of guanylyl-5'-imidodiphosphate (gppnhp) or naf-alcl3 than on that in the basal state. in the pertussis toxin-treated membranes, the effect of diazepam in the presence of gppnhp or naf-alcl3 was mar ... | 1988 | 2828793 |
| differential effects of benzodiazepines on phospholipid methylation in hippocampus and cerebellum of rats. | to elucidate the relationship between the occupancy of bdz binding sites and phospholipid methylation in brain, we examined phosphatidylethanolamine-n-methyltransferase (pemt) activity in synaptosomes of rat hippocampi and cerebella in the presence of bdz ligands with different modes of action. we found that ro 5-4864, a specific ligand for "peripheral type" receptors, increased pl methylation in hippocampal and cerebellar synaptosomes. this effect was directly related to receptor occupancy, sin ... | 1988 | 2828796 |
| interaction between highly selective mu and delta opioids in vivo at the rat spinal cord. | possible interactions between mu- and delta-receptors in the rat spinal cord were studied using the radiant-heat-induced tail flick response and the highly selective mu- or delta-ligands: [nmephe3,d-pro4] morphiceptin(pl-17) and cyclic[d-pen2,d-pen5]enkephalin(dpdpe). intrathecal infusions of pl-17 (0.5 microgram/hr) for 5 days caused 61- and 6-fold shifts to the right of the dose-response curves for pl-17 and dpdpe, respectively. since pl-17 and dpdpe are highly selective agonists for mu- and d ... | 1986 | 2829017 |
| investigation of opioid receptor binding protein in adult and fetal mouse brain membranes by photoaffinity labeling. | 125i-[d-ala2,p-n3-phe4-met5]enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. the photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. a 46,000-da protein was specifically photo-labeled in the adult mouse brain membranes. the photolabeling of this protein was inhibited in the presence of [d-ala2,met5]enkephalin and a peptide ligand specific for the mu-opioid receptor. no specific ... | 1988 | 2829063 |
| [visualization of an ischiadic hernia using peritoneography]. | 2010 | 2829318 | |
| [genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus]. | transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ... | 1987 | 2830691 |
| [genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus]. | transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ... | 1987 | 2830691 |
| kirsten murine sarcoma virus abolishes interferon gamma-induced class ii but not class i major histocompatibility antigen expression in a murine fibroblast line. | the effect of infecting fibroblasts with kirsten murine sarcoma virus/murine leukemia virus (ki-msv/mlv) on constitutive and ifn-gamma-induced h-2 antigen expression was investigated. the fibroblasts used were two established cell lines (c3h10t1/2 and balb/c3t3) and fresh embryo fibroblasts from c3h mice. class i antigens were expressed constitutively by balb/c3t3; infection with mlv, msv or the two together had little effect on this constitutive expression. class i antigens (h-2k, h-2d) were st ... | 1988 | 2831293 |
| decreased surfactant level in the lung with oligohydramnios: a morphometric and biochemical study. | 2009 | 2831329 | |
| decreased surfactant level in the lung with oligohydramnios: a morphometric and biochemical study. | 2009 | 2831329 | |
| a possible pain control system in a non-mammalian vertebrate (a lizard, gekko gecko). | in a lizard (gekko gecko) the anterograde tracer pha-l was microiontophoretically applied to the predominantly serotonergic nucleus raphes inferior. extensive spinal projections from the rostral magnocellular part of this nucleus were demonstrated to the superficial layers of the dorsal horn and to the intermediate zone, more sparsely to the ventral horn. but, in addition, retrogradely labeled neurons were found in and just below a periventricular cell group in tegmentum mesencephali, i.e. the l ... | 2010 | 2831478 |
| non-cholinergic basal forebrain neurons project to the contralateral basal forebrain in the rat. | following injections of wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) or the fluorescent tracer fluoro-gold into the magnocellular preoptic area and the horizontal limb of the diagonal band, retrogradely labelled neurons were found in the homotopic region of the contralateral basal forebrain. labelled fibers apparently arising from these neurons travelled in the stria medullaris and the habenular commissure to terminate in the contralateral basal forebrain. although the neuro ... | 1988 | 2831483 |
| hot nodule after fine needle aspiration. | 2009 | 2832117 | |
| effect of cyclosporin a immunosuppression on primary lymphotropic herpesvirus infection in the guinea pig. | female guinea pigs pretreated for 2 days with cyclosporin a (csa), were then inoculated intranasally (in) or intraperitoneally (ip) with a lymphotropic herpesvirus (gphlv) and followed by 4 additional daily doses of csa. immunosuppressed animals did not show lymphocytosis and virus infectivity titers in their spleen, cervical lymph node and blood mononuclear cells were lower than those of oil-treated controls. while in-inoculated csa-treated animals expressed higher virus infectivity titer in th ... | 1987 | 2832345 |
| nmr contrast agents for proton imaging. | 2010 | 2832350 | |
| cloning and expression of the vibrio cholerae neuraminidase gene nanh in escherichia coli. | a cosmid gene bank of vibrio cholerae 395, classical ogawa, was screened in escherichia coli hb101 for expression of the vibrio neuraminidase (nanase) gene nanh (n-acylneuraminate glycohydrolase). positive clones were identified by their ability to cleave the fluorogenic nanase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. seven nanase-positive clones were detected after screening 683 cosmid isolates with a rapid, qualitative plate assay method. the nanh gene was subcloned ... | 1988 | 2832365 |
| characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline ti plasmid ptic58. | overlapping segments of ptic58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine a and b agrobacterium tumefaciens strain c58. all of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines a and b. transposon tn3-hoho1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines a and b and ag ... | 1988 | 2832379 |
| rapid expression of ornithine decarboxylase mrna in a macrophage-like cell line: camp repression of the requirement for prior protein synthesis. | ornithine decarboxylase (odc) activity was rapidly induced in the raw264 macrophage-like cell line after treatment with bacterial lipopolysaccharide (lps). odc mrna levels were determined by isolating cellular rna, followed by northern blot and dot blot analysis using a 32p-labeled cdna probe. odc mrna levels increased within 1 hour of stimulation of raw264 cells with 1.0 microgram/ml lps. transcription rate analysis on isolated nuclei indicated that an increase in transcription rate contributed ... | 2010 | 2832424 |
| glucose and carbachol generate 1,2-diacylglycerols by different mechanisms in pancreatic islets. | diacylglycerols (dag) modulate secretory responses by the activation of protein kinase c. early changes in dag formation induced by the muscarinic receptor agonist carbachol were compared to those caused by the nutrient secretagogue glucose in pancreatic islets. turnover rates of dag were investigated in radiolabeling experiments, whereas changes in total mass and fatty acid composition of dag were assessed by gas-liquid chromatography. when islet lipids were labeled to steady state in tissue cu ... | 1988 | 2832445 |
| isolation of a simian virus 40-simian agent 12 recombinant papovavirus that synthesizes a hybrid large tumour antigen. | the simian virus 40 (sv40) mutant dl1055 carries a 55 base pair deletion in the viral early region coding sequences that causes premature termination of large tumour (t) antigen translation resulting in a protein fragment consisting of the amino-terminal 399 residues. the mutation renders the virus defective. we report the characterization of hyb4001, which was isolated as a single plaque following cotransfection of permissive cells with dl1055 dna and the dna of a related papovavirus, simian ag ... | 1988 | 2832530 |
| effects of spiraprilic acid, an angiotensin converting enzyme inhibitor, on large artery compliance in anesthetized dogs. | the present study examined the actions of spiraprilic acid, a new non-sulfhydryl angiotensin converting enzyme (ace) inhibitor upon aortic compliance (ac) in anesthetized dogs. enalaprilic acid was examined for comparative purposes. ac was determined via sonomicrometric determination of the ratio of aortic systolic-diastolic diameter (mm) and arterial pulse pressure (mmhg). one ac unit (acu) equals 10(-3) mm/mmhg. in non-thiazide pretreated animals, spiraprilic acid (1 mg/kg i.v.), caused a sust ... | 1987 | 2833190 |
| reversal by cyclic amp of the urea-induced inhibition of synthesis of a catabolite-repressible enzyme in vibrio cholerae. | low concentrations of urea, which did not inhibit the synthesis of the catabolite nonrepressible enzyme alkaline phosphatase in vibrio cholerae, or markedly affect its overall growth, specifically inhibited the expression of the tryptophanase operon in a temperature-dependent manner. however, in contrast to what is found in escherichia coli, this urea-induced inhibition of tryptophanase synthesis in v. cholerae could be almost completely relieved by exogenously added cyclic amp. the possible mec ... | 1987 | 2833565 |
| [a new inflammatory muscular disease: inclusion-body myositis]. | 2009 | 2835126 | |
| initial clinical experience with degradable starch microspheres. | four patients with advanced hepatic malignancy have been investigated with a view to intra-arterial treatment with degradable starch microspheres (dsm) and mitomycin-c (mmc). computer-assisted interpretation of data gathered during the administration of technetium-labelled albumin allows the degree of arteriovenous shunting within the liver to be measured. in a similar way, the effectiveness of dsm in blocking hepatic arterial flow is determined. two patients showed unacceptably high levels of s ... | 2009 | 2835715 |
| initial clinical experience with degradable starch microspheres. | four patients with advanced hepatic malignancy have been investigated with a view to intra-arterial treatment with degradable starch microspheres (dsm) and mitomycin-c (mmc). computer-assisted interpretation of data gathered during the administration of technetium-labelled albumin allows the degree of arteriovenous shunting within the liver to be measured. in a similar way, the effectiveness of dsm in blocking hepatic arterial flow is determined. two patients showed unacceptably high levels of s ... | 2009 | 2835715 |
| a novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in vibrio cholerae requires toxr. | the toxr gene of vibrio cholerae encodes a transmembrane, dna-binding protein that activates transcription of the cholera toxin operon and a gene (tcpa) for the major subunit of a pilus colonization factor. we constructed site-directed insertion mutations in the toxr gene by a novel method employing the chromosomal integration of a mobilizable suicide plasmid containing a portion of the toxr coding sequence. mutants containing these new toxr alleles had an altered outer membrane protein profile, ... | 1988 | 2836362 |
| adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf. | we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ... | 1988 | 2836559 |
| adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf. | we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ... | 1988 | 2836559 |
| [significance and practice of recall/after care]. | 2009 | 2836949 | |
| treatment of cytomegalovirus infections in renal transplant recipients with 9-(1,3-dihydroxy-2-propoxymethyl) guanine. | 1988 | 2837848 | |
| immune regulation by leukotriene b4. | 2010 | 2837957 | |
| insulinoma. the value of intraoperative ultrasonography. | after establishing the diagnosis of an insulinoma, most surgeons prefer preoperative localization. selective arteriography has long remained the gold standard for this purpose, but its use has been met with variable success. despite various attempts at localization, insulinomas remain undetected in 10% to 20% of patients, and there may be a postoperative complication rate of at least 10% to 25%. to review the results and surgical implications of current localization techniques, 36 adult patients ... | 2010 | 2838008 |
| immune response of chickens inoculated with a recombinant avian leukosis virus. | specific-pathogen-free embryos (18-day incubation) and hatched chicks were inoculated with a recombinant avian leukosis virus (alv) produced by recombinant dna techniques. enzyme-linked immunosorbent assays were used to measure the production of viral-protein-specific antibody and the viral protein, p27, in the serum at 2, 5, 8, 14, and 20 weeks of age. of the inoculated chickens surviving to 20 weeks, 64% produced viral-protein-specific antibodies and 42% transiently produced the viral protein, ... | 1988 | 2838018 |
| mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis. | in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ... | 1988 | 2838023 |
| absence of an endogenous regulator of na+,k+-atpase activity in the tissues of cirrhotic rats. | microsomal na+,k+-atpase isolated from the renal cortex of rats with ccl4-induced cirrhosis (cir) showed a higher specific activity than the enzyme obtained from control rats (cor). kinetic studies showed a lower k0.5 for atp (0.08 +/- 0.03 vs. 0.24 +/- 0.04 mm; p less than 0.05), a lower na+ activation constant (9.6 +/- 1.5 vs. 19.0 +/- 1.7 mm; p less than 0.05), and a higher k+ activation constant (1.2 +/- 0.1 vs. 0.6 +/- 0.1 mm; p less than 0.05) for cir. the optimal ph of the enzyme was 0.5 ... | 1988 | 2838057 |
| evidence for a functional defect in the translocation of the methotrexate transport carrier in a methotrexate-resistant murine l1210 leukemia cell line. | properties of the methotrexate (mtx) transport carrier were examined in a stable single-step 16-fold mtx-resistant l1210 murine leukemia cell line with unchanged dihydrofolate reductase gene copy and thymidylate synthase and dihydrofolate reductase levels and activities. mtx influx was markedly depressed due to a decrease in vmax without a change in km. from this cell line a clonal variant with greater resistance to mtx was identified due solely to a further decrease in influx vmax. trans-stimul ... | 2006 | 2838483 |
| dynamic liver scanning in cirrhosis. | dynamic hepatic scintigraphy was performed in 49 patients with established cirrhosis, using intravenous 99tcm-pertechnetate and 99tcm-sulphur colloid in a prospective study of its predictive value. there was a close correlation between the hepatic perfusion index (reflecting the ratio of arterial to total hepatic blood flow) obtained with pertechnetate (hpi-p) and with sulphur colloid (hpi-c) (r = 0.775; p less than 0.0001), and both indices correlated with disease severity (hpi-p p less than 0. ... | 2010 | 2838775 |
| [biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury]. | 2010 | 2838873 | |
| [opioid peptides and their possible physiological significance]. | 2009 | 2839888 | |
| [role of the leukotrienes in asthma]. | leukotrienes (lt), which are derived from arachidonic acid and have similar chemical structures to the prostaglandins, have recently been shown to contain most of the biological activity previously attributed to "slow reacting substance of anaphylaxis" (srs-a). ltc4, ltd4 and lte4 have potent bronchoconstrictor actions. lte4 causes very long-lasting bronchoconstriction. ltb4 has inflammatory properties and possesses powerful chemotactic activity on leukocytes, especially neutrophil polymorphonuc ... | 2010 | 2840090 |
| human and simian immunodeficiency retroviruses: activation and differential transactivation of gene expression. | new west african human immunodeficiency viruses (hiv-2s) and simian immunodeficiency virus (siv) contain functional transactivator (tat) gene and tat response elements. their long terminal repeats (ltr) and tat genes are more related among themselves than to hiv-1 ltr and tat gene. the viral gene expression of hiv-2 as well as siv can be stimulated by t cell activators, such as mitogens and phorbol esters. hiv-2 and siv display a much broader transactivation response specificity than does hiv-1. ... | 1988 | 2840105 |
| cannabis consumption in catalonia, spain. | 2010 | 2840140 | |
| selective inhibition of arachidonate 5-lipoxygenase by novel acetohydroxamic acids: biochemical assessment in vitro and ex vivo. | 1. the chemically novel acetohydroxamic acids, bw a4c, bw a137c and bw a797c, are potent inhibitors of the synthesis of leukotriene b4 (ltb4) from arachidonic acid by human leucocyte homogenates: the concentrations required for 50% inhibition (ic50) were 0.1 microm, 0.8 microm and 0.5 microm respectively. inhibition was less at higher concentrations of arachidonic acid. 2. these compounds also inhibited the synthesis of [14c]-5-hete from [14c]-arachidonic acid and the calcium-dependent synthesis ... | 1988 | 2840160 |
| developmental changes in myosin isoforms from slow and fast latissimus dorsi muscles in the chicken. | in the course of muscle differentiation, changes in fibre-type population and in myosin composition occur. in this work, the expression of native myosin isoforms in developing fast-twitch (posterior latissimus dorsi; pld) and slow-tonic (anterior latissimus dorsi; ald) muscles of the chick was examined using electrophoresis under nondissociating conditions. the major isomyosin of 11-day-old embryonic pld comigrated with the adult fast myosin fm3. two additional components indistinguishable from ... | 1988 | 2840316 |
| a new hamster model for adenoviral vaccination. | both adult and baby hamsters infected intranasally with human adenovirus type 5 exhibited virologic, serologic, and histologic evidence of infection. when 8-day old hamsters were infected with 4 x 10(6) pfu, concentrations of virus up to 2 x 10(6) pfu/animal were detected in the lung, peaking on day 2. the minimum infectious dose was 1 x 10(3) pfu/animal. this model may be useful in studies of conventional and recombinant adenoviral vaccines for humans. | 1988 | 2840876 |
| effect of parathyroid hormone and forskolin on cytoskeletal protein synthesis in cultured mouse osteoblastic cells. | parathyroid hormone (pth) has been shown to cause transient cell shape changes in bone cells. we have examined the effects of parathyroid hormone and forskolin on the organization and expression of cytoskeletal proteins in cultured mouse endosteal osteoblastic cells. analysis of [35s]methionine-labeled cytoskeletal proteins isolated on two-dimensional gel electrophoresis showed that pth treatment (24 h) stimulated the de novo biosynthesis of actin, vimentin and tubulins in confluent cells, where ... | 1988 | 2840972 |
| molecular defects in cytochrome oxidase in mitochondrial diseases. | defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ... | 1988 | 2841309 |
| molecular defects in cytochrome oxidase in mitochondrial diseases. | defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ... | 1988 | 2841309 |
| egg shell quality and cholecalciferol metabolism in aged laying hens. | calcium-binding protein d28k (calbindin) synthesis, vitamin d metabolism and shell quality were investigated in young and aged laying hens fed diets containing either cholecalciferol (cc) or its 1-hydroxylated derivatives. duodenal calbindin concentration was similar in the young and in the aged laying hens. exogenous 1-hydroxylated cc derivatives increased duodenal calbindin concentration, regardless of age. shell weight and shell density (mg/cm2) were significantly lower (p less than 0.01) in ... | 1988 | 2841436 |
| primary mediastinal germ cell tumors. histologic patterns of treatment failures at autopsy. | twenty-five patients presented with primary mediastinal germ cell tumors at roswell park memorial institute between 1959 and 1984. all patients were treated by surgery and chemotherapy with or without radiotherapy. four patients are still alive, and 21 patients died of mediastinal germ cell tumor and its sequelae. two patients were found to have testicular scars and were dropped from the study. nongerm cell malignant transformation of a teratoma occurred in five of the remaining 17 patients (29% ... | 2010 | 2842031 |
| cyclic nucleotide phosphodiesterase activity: histochemical and cytochemical methods. | 2009 | 2842613 | |
| cyclic nucleotide phosphodiesterase activity: histochemical and cytochemical methods. | 2009 | 2842613 | |
| thiol peroxyl radical formation from the reaction of cysteine thiyl radical with molecular oxygen: an esr investigation. | using electron spin resonance (esr) spectroscopy, we have identified the cysteine thiol peroxyl radical (cyssoo.) at low temperatures in two aqueous glasses. this radical shows a typical peroxyl radical esr spectrum, but unlike carbon-based peroxyl radicals has a violet color (lambda max = 540 nm) and forms a new radical showing a singlet esr spectrum when photobleached with visible light. the cysteine peroxyl radical reacts to form the cysteine sulfinyl radical (cysso.) in the glass which allow ... | 2010 | 2843184 |
| rna polymerase ii allows unwinding and rewinding of the dna and thus maintains a constant length of the transcription bubble. | ternary complexes of rna-dna-rna polymerase ii, originating from the in vivo transcriptionally active sv40 minichromosomes, can be detected and analyzed by a method previously developed (choder, m., bratosin, s., and aloni, y. (1984) embo j. 3, 2929-2936). using this method, we compared the electrophoretic mobilities of sv40 ternary complexes with those of sv40 rna-dna complexes obtained after the removal of the polymerases. independent of the in vitro elongation of the nascent rna, topoisomers ... | 2009 | 2843504 |
| percutaneous sonographic needle aspiration biopsy of endoscopically negative gastric carcinoma. | we report here the use of ultrasound-guided percutaneous fine needle aspiration gastric biopsies in three patients with endoscopically negative biopsies. two men, ages 60 and 64, and one woman, age 64, with signs and symptoms of weight loss, abdominal pain, and early satiety, had barium contrast studies suggestive of thickened gastric walls of the antrum and cardia, yet multiple endoscopic biopsies were negative for malignancy. using real time sector b-scan ultrasonography, percutaneous fine nee ... | 2007 | 2844079 |
| effect of ethanol on neuromuscular function in the rat diaphragm preparation. | the effect of ethanol on neuromuscular transmission in the isolated phrenic nerve-diaphragm preparation of the rat was studied by analysing its effect on directly- and indirectly-elicited twitch tension, tetanic and post-tetanic twitch tensions, and on the phenomenon of post-tetanic twitch potentiation (ptp). the results show that ethanol (1.0 x 10(-4)-1.0 m) depresses neuromuscular transmission, by reducing the amplitudes of both directly and indirectly elicited twitch and tetanic contractions. ... | 1988 | 2844197 |
| [relation between the characteristic activity of the beta-adrenergic agonist isoproterenol and the percolation properties of cell membrane]. | variations in the intrinsic activity of 1-isoproterenol and in the percentage of high affinity beta-receptor complexes have been studied under a changes in the macrostructure of reticulocyte plasma membranes. the fluid lipid fraction have been reduced in the membranes for this by phospholipase a2/bsa treatment. it was accompanied by a progressive decrease in the fraction of beta-receptors that are able to form the high affinity complexes with 1-isoproterenol (receptor--regulatory n-protein compl ... | 1988 | 2844324 |
| detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration. | the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ... | 1988 | 2844405 |
| extraretroperitoneal abdominal malignant fibrous histiocytoma. | seven cases of extraretroperitoneal abdominal malignant fibrous histiocytoma (mfh) are reported. the anatomic distribution was as follows: liver (2 cases), spleen with gastric and pancreatic involvement (1 case), mesentery (1 case), right mesocolon (1 case), left mesocolon (1 case), and urachus (1 case). these bulky lesions were often extensively necrotic; calcifications were seen in 2 cases. all 7 cases were investigated by ultrasonography (us) and computed tomography (ct). barium studies were ... | 2010 | 2844619 |
| automated synthesis system with computer control for the production of [1-11c]fatty acids. | to accommodate the increasing need for [1-11c]fatty acids for use in routine medical studies and the large amounts of radioactivity required, a more advanced automated synthesis system for the preparation of [1-11c]fatty acids has been constructed. the synthesis of [1-11c]fatty acids has been completely automated using a new separation unit (isobaric sepaltor) for the extraction procedure, which had caused some difficulties with automation. the yield of [1-11c]palmitic acid was 20-30 mci, and th ... | 2010 | 2844700 |
| t4 cell activation by immobilized phytohemagglutinin: differential capacity to induce il-2 responsiveness and il-2 production. | soluble mitogens, such as pha induce accessory cell (ac)-dependent t cell proliferation. one function of the ac is to create a stimulatory matrix. therefore, experiments were carried out to determine whether pha immobilized onto microtiter plates could stimulate t cells in the absence of ac. peripheral blood t4 cells were cultured under limiting dilution conditions with either soluble or immobilized pha with or without ril-1 beta, ril-2, r-tnf-alpha, an anti-cd28 mab (9.3), or irradiated ebv-tra ... | 1988 | 2844904 |