Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| hybrids of escherichia coli and shigella flexneri 1b. | 1966 | 5331420 | |
| development of h-sh and p1kcvir phages in shigella flexneri f6s studied by fluorescence microscopy and electron microscopy. | 1966 | 5333382 | |
| [studies on the duration of life of shigella flexneri and shigella sonnei in artificially infected milk and milk products]. | 1965 | 5333548 | |
| [the resistance of antigenic components of shigella flexneri. 3. the effect of merthiolate and other chemical substances on the antigenic properties of microbes]. | 1969 | 5372771 | |
| [antigenic conversions in shigella flexneri. 2. effects of lysogenization of serotype 1, serotype 2, variant x and variant y cultures by a phage isolated from a serotype 5 culture]. | 1969 | 5373656 | |
| new scheme of phage typing of shigella flexneri. | 1969 | 5398949 | |
| study of the structure of shigella flexneri o antigen. i. chemical degradation. | 1970 | 5418717 | |
| study of the structure of shigella flexneri o antigen. ii. physicochemical aspect. | 1970 | 5418718 | |
| chemotherapy of mice experimentally infected with shigella flexneri. | a chronic infection with shigella flexneri 2a has been established in mice for the evaluation of compounds for therapeutic potential. evidence of infection is indicated by prolonged symptomless excretion in the feces and by positive isolation of organisms from different segments of the intestinal tract and from mesenteric lymph nodes. serum antibody titer reaches a maximum after 9 days of infection and remains at a low level until 32 days postinfection. in this model, five drugs used in human sh ... | 1970 | 5422307 |
| isolation and selection of auxotrophic and fermentative mutants of shigella flexneri. | 1970 | 5455473 | |
| [immunochemistry of shigella. 3. specific polysaccharides of shigella flexneri types: 2,3 and 6]. | 1970 | 5459382 | |
| shigella flexneri dysentery in the australian forces in south vietnam. | 1970 | 5467372 | |
| chemoprophylaxis of shigella flexneri keratoconjunctivitis in rabbits. | chloramphenicol, chlortetracycline, streptomycin, and sulfisoxazole prevented keratoconjunctivitis in rabbit eyes infected with shigella flexneri. the route of administration of the agent and the dose were important variables in controlling the infectious process. this assay system appears to be a useful technique for the in vivo evaluation of chemotherapeutic agents. | 1970 | 5485732 |
| morphology of bacteriophages used for typing shigella flexneri. | 1970 | 5493081 | |
| the immunochemistry of shigella flexneri o-antigens: a new serologically active trisaccharide from variant y. | 1970 | 5500288 | |
| [shigella flexneri f6s with extended patterns of sensitivity to the t phages and to colicins after infection by a lisbonne phage. 1. modified biological properties of the bacterial surface]. | 1971 | 5564194 | |
| concerning the classification of shigella flexneri 6 bacilli. | 1967 | 5588833 | |
| s-r dissociation in shigella flexneri populations. 3. respiration of smooth and rough forms. | 1967 | 5588835 | |
| phage typing, colicin sensitivity and colicinogeny of shigella flexneri 6. | 1967 | 5591942 | |
| the immunochemistry of shigella flexneri o-antigens: an analysis of the immunodominant sugars in lipopolysaccharides from smooth strains. | 1968 | 5650132 | |
| the immunochemistry of shigella flexneri o-antigens a study of smooth to rough mutation. | 1968 | 5664129 | |
| lysotypes of shigella flexneri. | 1968 | 5664130 | |
| resistance of shigella flexneri to antibiotics. | 1968 | 5666680 | |
| phage typing of shigella flexneri. | 1968 | 5666687 | |
| transferable multiple drug resistance in enterobacteria from non-human primates. i. development and characteristics of resistance in shigella flexneri. | 1968 | 5667656 | |
| [stability of the antigenic components of shigella flexneri. i]. | 1968 | 5729700 | |
| the response of man to virulent shigella flexneri 2a. | 1969 | 5780532 | |
| [stability of the antigenic components of shigella flexneri. ii. supplement to the characteristics of type antigens i, 3 and v]. | 1969 | 5784553 | |
| response and specificity of antibodies for shigella flexneri: demonstration of type-specific factors in immunoglobulin g fraction of antiserum. | considerable amounts of immunoglobulin g (igg) antibody appeared in hyperimmune rabbit serum at a late period during a course of immunization with several injections of shigella flexneri o antigens. high yields of igg antibody possessing homogenous specificity could be fractionated from crude gamma-globulin solution on a diethylaminoethyl-sephadex column with 0.02 m phosphate buffer (ph 6.6) containing 0.1 m nacl. specificities of igg antibodies for six serotypes of s. flexneri were demonstrated ... | 1969 | 5788700 |
| circular dna from shigella paradysenteriae. | 1969 | 5804495 | |
| [extraordinary place of discovery (vagina) of shigella flexneri]. | 1969 | 5804669 | |
| the immunochemistry of shigella flexneri o-antigens: a comparative analysis of the o-specific side-chain structures of serotypes 1a and 2a. | 1969 | 5810099 | |
| [lysotyping of shigella. v. provisional scheme for lysotyping of shigella flexneri type 3a]. | 1964 | 5830282 | |
| the mechanism of antagonism between antigenic types of dysentery bacilli of the shigella flexneri subgroup. | 1965 | 5837179 | |
| [study on the streptomycin-dependent shigella flexneri strain for oral vaccination against dysentery]. | 1965 | 5848528 | |
| [shigella flexneri in cat experiments]. | 1965 | 5860519 | |
| survival of shigella in sewage. 3. effect of glycerol on intracellular growth of bacteriophage in shigella flexneri. | 1965 | 5867663 | |
| shigella flexneri serovariants containing combinations of type antigens. | 1965 | 5870704 | |
| [research on active immunity of man in bacillary dysentery. 3. maintenance of the non-pathogenic character of the shigella flexneri 2a txxxii strain orally administered to volunteers]. | 1965 | 5877879 | |
| the effect of dysentery bacteriophage on the course of experimental cystitis produced in guinea-pigs by shigella flexneri. | 1965 | 5893861 | |
| the immunochemistry of shigella flexneri lipopolysaccharides. a quantitative analysis of their monosaccharide constituents. | 1. the lipopolysaccharides of a representative selection of shigella flexneri serotypes all contain the same constituents as salmonella chemotype vii, namely, aldoheptose phosphate, 3-deoxy-2-oxo-octonate, o-phosphorylethanolamine, d-galactose, d-glucose, n-acetyl-d-glucosamine and l-rhamnose. 2. the presence of all the salmonella basal sugars in sh. flexneri lipopolysaccharides is consistent with the view that the latter contain a basal structure or core which is similar to the common basal str ... | 1966 | 5911535 |
| shigella flexneri bacteraemia: report of a case. | 1966 | 5947868 | |
| [proposition to change the serologic designation of types and subtypes of shigella flexneri]. | 1966 | 5959783 | |
| [shigella flexneri bacteremia]. | 1966 | 5967209 | |
| shigella flexneri f6s strains with acquired sensitivities to the t phages after infection by lisbonne phages. | 1966 | 5970172 | |
| [antidysenteric vaccination. xiv. immunizing action on the guinea pig conjunctiva of various toxic extracts of pathogenic shigella flexneri 2a ti, as compared with that of live cultures of two non-pathogenic strains of shigella flexneri 2a]. | 1966 | 5973711 | |
| [lysotyping of shigella flexneri strains 2a and 3a isolated from several epidemic foci in rumania]. | 1966 | 5981233 | |
| [antidysenteric vaccination. xv. immunogenicity of various strains of shigella schmitzi shigella flexneri 1b, 2b, 3a, 4, 6, shigella boydii and shigella sonnei "s" in relation to the immunogenicity of a keratoconjunctivitis positive enterobacterium lacking shigella antigens (agglutinogens) (strain "well 3")]. | 1966 | 5985458 | |
| [studies on the antigen structure of shigellae. 1. studies on the chemical composition of lipopolysaccharides of a shigella flexneri s and r form and comparison with a salmonella r form]. | 1966 | 5994748 | |
| inhibition of shigella flexneri by the normal intestinal flora. i. mechanisms of inhibition by klebsiella. | growth curves were plotted for shigella flexneri and klebsiella (aerobacter aerogenes) multiplying in pure and mixed culture. in mixed culture, klebsiella inhibited shigella. exponential growth was interrupted and shigella entered into a logarithmic death phase. an analysis of cultures at the time inhibition occurred revealed that formic and acetic acids produced by klebsiella were responsible for the inhibition of shigella. klebsiella strongly reduced the culture medium. the volatile fatty acid ... | 1967 | 6032512 |
| immunochemical studies on shigella flexneri polysaccharides. | 1967 | 6050439 | |
| studies on the virulence of shigella flexneri. | 1967 | 6057577 | |
| interference of lysogenic shigella flexneri with antimicrobial disc susceptibility readings. | 1967 | 6058731 | |
| the immunochemistry of shigella flexneri o-antigens. the biochemical basis of smooth to rough mutation. | 1. smooth to rough mutation has the same biochemical basis in shigella as in salmonella. it is the result of enzyme defects blocking the incorporation of the o-specific side chains that characterize the smooth lipopolysaccharide with the consequent exposure of the underlying basal structures that determine ;rough'-specificity. 2. the shigella flexneri basal structure resembles its salmonella analogue in that it has the same qualitative sugar composition, and enzyme defects in its biosynthetic pa ... | 1967 | 6060453 |
| [role of specific bacteriophage in the control of the carrier state of shigella flexneri 6 bacilli]. | 1967 | 6066184 | |
| [immunochemical analysis of oligosaccharide fragments of o-specific shigella flexneri polysaccharides]. | comparison of inhibitory properties of several synthetic oligosaccharides related to sh. flexneri o-specific polysaccharides, namely glc alpha 1-3rha alpha 1-ome, glc alpha 1-3rha alpha 1-2rha alpha 1-ome, rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome, glcnac beta 1-2(glc alpha 1-3)rha alpha 1-ome, and glcnac beta 1-2(glc alpha 1-3) rha alpha 1-2rha alpha 1-ome, using passive haemagglutination reaction demonstrated the tetrasaccharide to possess the highest activity in v; 7,8-anti-7,8 immune syste ... | 1984 | 6083795 |
| glycocalyx visualization in shigella flexneri. | 1984 | 6085609 | |
| [in vitro activity of a new cephalosporin (ceftriaxone) on recently isolated strains of shigella]. | 1984 | 6088342 | |
| virulence of entamoeba histolytica trophozoites. effects of bacteria, microaerobic conditions, and metronidazole. | the association of axenically grown trophozoites of entamoeba histolytica strains hk-9 or hm-1:imss with various types of gram-negative bacteria for relatively short periods markedly increased their virulence, as evidenced by their ability to destroy monolayers of tissue-cultured cells. interaction of trophozoites with bacteria that were heat inactivated, glutaraldehyde fixed, or disrupted by sonication, or bacteria treated with inhibitors of protein synthesis, did not augment amebic virulence. ... | 1984 | 6088660 |
| cloning and characterization of reca genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r. | the reca genes of proteus vulgaris, erwinia carotovora, shigella flexneri and escherichia coli b/r have been isolated and introduced into escherichia coli k-12. all the heterologous genes restore resistance to killing by uv irradiation and the mutagen 4-nitroquinoline-1-oxide in reca- e. coli k-12 hosts. recombination proficiency is also restored as measured by formation of lac+ recombinants from duplicated mutant lacz genes and the ability to propagate phage lambda derivatives requiring host re ... | 1984 | 6090408 |
| aerobactin genes in shigella spp. | aerobactin, a hydroxamate iron transport compound, is synthesized by some, but not all, shigella species. conjugation and hybridization studies indicated that the genes for the synthesis and transport of aerobactin are linked and are found on the chromosome of shigella flexneri, s. boydii, and s. sonnei. the genes were not found in s. dysenteriae. a number of aerobactin synthesis mutants and transport mutants have been isolated. the most common mutations are deletions of the biosynthesis or bios ... | 1984 | 6090419 |
| mercuric ion-resistance operons of plasmid r100 and transposon tn501: the beginning of the operon including the regulatory region and the first two structural genes. | the mercuric ion-resistance operons of plasmid r100 (originally from shigella) and transposon tn501 (originally from a plasmid isolated in pseudomonas) have been compared by dna sequence analysis. the sequences for the first 1340 base pairs of tn501 are given with the best alignment with the comparable 1319 base pairs of r100. the homology between the two sequences starts at base 58 after the end of the insertion sequence is-1 of r100. the sequences include the transcriptional regulatory region, ... | 1984 | 6091128 |
| evidence for clustering of rna polymerase and ribosomal protein genes in six species of enterobacteria. | 32p-labelled dna fragments from the e. coli beta operon were used as hybridisation probes for homologous dna sequences in chromosomal digests of six species of enterobacteria. in all species the hybridisation pattern suggested clustering of the genes comprising the beta operon. | 1984 | 6092848 |
| periodontal disease associated with shigella flexneri in rhesus monkeys. clinical, microbiologic and histopathologic findings. | 1982 | 6124590 | |
| genetic transfer of a mucosal adherence factor (r1) from an enteropathogenic escherichia coli strain into a shigella flexneri strain and the phenotypic suppression of this adherence factor. | escherichia coli strain rdec-1 avidly adheres to rabbit ileal brush borders. two separate experiments were designed to determine whether pili promote this adherence. (1) adherence of strain rdec-1 was phenotypically suppressed by changing the culture medium. loss of adherence was correlated with the absence of pili. thus, growth of strain rdec-1 in penassay broth (difco laboratories, detroit, mich.) promoted both adherence and expression of pili on greater than or equal to 90% of organisms, wher ... | 1983 | 6132950 |
| antigenic relationship among virulent enteroinvasive escherichia coli, shigella flexneri, and shigella sonnei detected by elisa. | 1983 | 6134933 | |
| immunologic observations in macaques with shigella-associated periodontal disease. | 1983 | 6135769 | |
| changes in phage-sensitivity of shigella flexneri strains. ii. the f-like character of ms2-sensitive variants. | variants sensitive to male specific phage ms2 appeared among ms2 non-sensitive shigella flexneri cultures in the course of liquid medium passages. sensitivity to ms2 was lost on acridine orange treatment and was transferable into ms2 non-sensitive variants. the ms2 sensitive variants had fimbrial antigen. electron microscopy showed that ms2 phages were adsorbed on the f-like fimbriae of these variants. it was assumed that f-like plasmids determining f-like fimbrial antigen were carried by these ... | 1983 | 6140808 |
| peyer's patch lymphoid follicle epithelial adherence of a rabbit enteropathogenic escherichia coli (strain rdec-1). role of plasmid-mediated pili in initial adherence. | escherichia coli (strain rdec-1) adheres to m cells of rabbit peyer's patch lymphoid follicle epithelium. the rdec-1 strain contains an 85 x 10(6) d plasmid that codes for pili, which, when purified, adhere to gut absorptive epithelium. this study compared the in vivo lymphoid follicle adherence of the rdec-1 strain with that of a shigella flexneri (shd15) that contained the 85 x 10(6) d plasmid and expressed the rdec-1 pili, a control e. coli, and a control s. flexneri (shd12). the bacteria wer ... | 1984 | 6145724 |
| [the conjugation system determined by plasmid r477 and other plasmids belonging to the h2 incompatibility group]. | 1984 | 6148780 | |
| early colonic lesions in experimental shigella infection in rhesus monkeys: revisited. | rhesus monkeys (macaca mulatta), given 3 x 10(8) to 5 x 10(10) shigella flexneri 2a orally, developed signs of acute shigellosis within 24 hours. a diffuse acute colitis was well established at 48 hours. the inflammatory reaction was confined to the mucosa. the submucosa showed only edema. the shigellae were found predominantly in the columnar cells of the surface epithelium, less frequently in those of the crypt, and least frequently in the lamina propria. shigella bacilli invaded the columnar ... | 1982 | 6153000 |
| stimulation of prostaglandin e production by bacterial endotoxins in cultured human synovial fibroblasts. | e coli, shigella, salmonella, and cholera endotoxins stimulated prostaglandin e (pge) production by cultured human synovial and foreskin fibroblasts. the minimal effective dose of shigella endotoxin was 2 micrograms/ml and a maximal response was observed at 10 micrograms/ml. pge stimulation was first detected 7 hours after addition of cholera endotoxin. stimulation by shigella endotoxin of both pge and hyaluronic acid production was inhibited by indomethacin and aspirin. the present results sugg ... | 1980 | 6157394 |
| serological analysis of core oligosaccharides of shigella flexneri serotype 6 and its r mutants lipopolysaccharides. | the core oligosaccharides originating from shigella flexneri 6 s strains and r mutants were examined by the complement fixation inhibition and passive hemolysis inhibition tests using antisera against the complete and partially degraded core regions. strong cross reactivity between shigella flexneri 6, shigella sonnei phase ii and e. coli c core fractions which all are of r1 type was observed. terminal beta-d-glucosyl groups and alpha-d-galactosyl-1,2-alpha-d-galactosyl sequences are essential e ... | 1981 | 6181755 |
| [obtaining dysentery and salmonellosis diagnostica for the coagglutination reaction in lyophilized form and the elaboration of a rapid system]. | 1982 | 6182376 | |
| [immunoelectrophoresis analysis of the antigenic composition of e. coli k12 hybrids which acquired the serological specificity of s. newcastle and s. flexneri]. | genetic and immunoelectrophoretic studies confirm earlier data on the presence of 2 specific antigens of acidic nature in s. newcastle; one of them is a specific thermolabile k-antigen responsible for type iv specificity of these bacteria. the data concerning the differences in the genetic determinants controlling the synthesis of o- and k-antigens in s. newcastle have been obtained. s. newcastle o- and k-antigens did not react with s. flexneri in the group serum system 3, 4, which indicates tha ... | 1982 | 6184914 |
| hela cell invasiveness and o antigen of shigella flexneri as separate and prerequisite attributes of virulence to evoke keratoconjunctivitis in guinea pigs. | many rough mutants selected from isogenic smooth virulent and avirulent strains of shigella flexneri were examined for virulence, using tissue culture infection and sereny tests. many of the rough mutants isolated from a virulent smooth strain were capable of penetrating tissue culture cells but incapable of producing a positive sereny test. in contrast, we could not obtain from smooth avirulent strains any rough mutants capable of penetrating hela cells. chemical analysis of lipopolysaccharide ... | 1983 | 6187681 |
| [stimulation of replication and increase in virulence of shigella growing in rna-containing media]. | 1983 | 6188298 | |
| [causes of the nonspecific reactions with antigenic erythrocyte diagnostic reagents]. | the causes leading to the appearance of positive reactions in diagnostic titers (1:200 and higher), registered in the sera of healthy persons in tests carried out with the use of experimental and commercial lots of shigella sonnei and shigella flexneri antigenic diagnostic reagents, are analyzed. the study has shown that the appearance of nonspecific reactions may be due to the influence of the initial state of the microbial culture (the biomass used as raw material for the production of sensiti ... | 1983 | 6191477 |
| [a new antigenic variety of shigella flexneri]. | 1983 | 6194615 | |
| [use of the coagglutination reaction for identifying shigella flexneri]. | the data on the use of the coagglutination test for the identification of sh. flexneri are presented. the influence of different methods for the treatment of antisera on their heterologous reactions was studied and the advantage of coagglutinating reagents was revealed. 9 variants of coagglutinating preparations were obtained; their use made it possible to reduce the consumption of antisera and in some cases avoid their absorption. the problem of sh. flexneri classification is discussed. | 1983 | 6194634 |
| [quantitative determination of the soluble antigens of intestinal bacteria using a method of immunoenzyme analysis. i. processing of the parameters of the method]. | the parameters of the assay based on the quantitative evaluation of the neutralization of specific antibodies by the antigen under study and the subsequent detection of free antibodies on the fixed reference antigen with the aim of the quantitative determination of the specific o-antigens of salmonella, groups d, b, c1, as well as those of shigella sonnei and shigella flexneri, have been developed. the proposed method makes it possible to detect the o-antigen of the causative agent at concentrat ... | 1983 | 6195847 |
| monoclonal antibodies specific for o-antigenic polysaccharides of shigella flexneri: clones binding to ii, ii:3,4, and 7,8 epitopes. | hybrid cells producing monoclonal antibodies against the o-antigens of shigella flexneri were obtained by polyethylene glycol-mediated fusion of myeloma cells and lymphocytes from balb/c mice immunized with whole heat-killed s. flexneri bacteria of serotypes 2a and 2b. clones were selected for their binding specificity to structurally defined s. flexneri lipopolysaccharides (lps). the following three groups were identified as recognizing three different epitopes: monoclonal antibodies binding to ... | 1983 | 6196376 |
| the shigella flexneri o-antigenic polysaccharide chain. nature of the biological repeating unit. | the sequence of monosaccharides in the biological repeating tetrasaccharide unit of shigella flexneri variant y o-antigenic polysaccharide chain was determined by subjecting three oligosaccharides of the polysaccharide, obtained by phage-sf6-mediated enzymatic hydrolysis, to methylation analysis and proton nuclear magnetic resonance spectroscopy. the smallest saccharide was shown to be a tetrasaccharide with the structure alpha-l-rhap-(1-2)-l-rha. the next saccharide, an octasaccharide, was show ... | 1984 | 6199198 |
| artificial carbohydrate antigens: a block synthesis of a linear, tetrasaccharide repeating-unit of the shigella flexneri variant y polysaccharide. | glycosylation of methyl 2,4-di-o-benzoyl-alpha-l-rhamnopyranoside with 2,3,4-tri-o-acetyl-alpha-l-rhamnopyranosyl bromide gave methyl 2,4-di-o-benzoyl-3-o-(2,3,4-tri-o-acetyl-alpha-l-rhamnopyranosyl) -alpha-l-rhamnopyranoside (4) in 93% yield. conversion of 4 into the corresponding glycosyl bromide was accomplished with dibromomethyl methyl ether. under koenigs-knorr conditions, this bromide reacted with 8-(methoxycarbonyl)octyl 2-o-(2-acetamido-4,6-o-benzylidene-2-deoxy-beta-d-glycopyranosyl)- ... | 1983 | 6200226 |
| [clinico-diagnostic significance of specific antigenemia in acute dysentery]. | 1984 | 6205453 | |
| [characteristics and trends in the development of an epidemic process of sonne and flexner dysentery among preschool children]. | the results of studies indicate that the morbidity rates of dysentery among children attending preschool institutions and children brought up at home converged in recent years. this phenomenon was most pronounced among children of the kindergarten age group. at the same time dysentery caused by sh. sonnei and sh. flexnery produced a higher morbidity rate among children attending nursery in comparison with that among children of the same age group brought up at home. group infections in preschool ... | 1982 | 6211010 |
| [manifestations of the cyclic nature of the epidemic process in dysentery]. | different forms of dysentery, especially those caused by sh. sonnei and sh. flexneri, have been found to differ considerably in their cyclic recurrence. the development cycles of the epidemic processes of dysentery have an objective character, occur in the presence of any tendencies in the morbidity rate, and depend on the natural factors. thus, the cycles of increase and decrease in morbidity are 3, 6, 9, 12 years for dysentery caused by sh. sonnei and 6, 7, 8 years for dysentery caused by sh. ... | 1982 | 6213120 |
| expression of hydroxamate and phenolate siderophores by shigella flexneri. | shigella flexneri strains were assayed for the ability to synthesize and utilize phenolate and hydroxamate siderophores. the hydroxamate aerobactin was synthesized by all isolates tested, whereas phenolates were only rarely produced. expression of aerobactin was accompanied by production of a single iron-regulated outer membrane protein (mr = 74,000). this protein was not produced by a mutant defective in aerobactin utilization and may serve as the aerobactin receptor. phenolate (enterobactin)-p ... | 1983 | 6224775 |
| [preventive effectiveness of dried polyvalent shigella bacteriophage in organized collective farms]. | 1984 | 6235671 | |
| bacteriophage mu d1(apr lac) generates vir-lac operon fusions in shigella flexneri 2a. | previous studies have demonstrated that expression of virulence in shigella spp. is controlled by growth temperature. to study the regulation of virulence (vir) genes, we set out to develop a rapid, easily-assayed phenotype with which to measure expression of virulence. this report described a procedure for isolating vir-lac operon fusions in s. flexneri 2a by using the specialized transducing bacteriophage mu d1(apr lac) of casadaban and cohen (m. casadaban and s. n. cohen, proc. natl. acad. sc ... | 1984 | 6236150 |
| [change in the nonesterified fatty acid makeup of shigella flexneri no. 15172 type 3a in the process of passage on developing chick embryos]. | composition of nonesterified fatty acids/nefaj from shigella flexneri was similar to that from other gram-negative bacteria of the enteritic group. at the same time, nefa of the dysenteric bacteria contained comparatively high amount of branched and cyclopropane-bearing fatty acids. adaptation of shigella flexneri in the medium of developing chicken embryo led to a distinct increase in total nefa content as well as of cyclopropane-bearing and saturated fatty acids in the bacteria and to a decrea ... | 1980 | 6245536 |
| genetic and physical evidence for plasmid control of shigella sonnei form i cell surface antigen. | virulent shigella sonnei synthesize a surface antigen (form i) which appears to be one of several requirements needed for this host to invade epithelial cells. upon restreaking on agar media, form i cells readily and irreversibly generate form ii cells that lack the form i antigen. all form ii cells are avirulent. plasmid deoxyribonucleic acid of form i and ii cells of four different s. sonnei isolates, obtained from different areas of the world, was analyzed by agarose gel electrophoresis. a la ... | 1980 | 6249756 |
| isolation of two protein-free and chemically different lipopolysaccharides from bordetella pertussis phenol-extracted endotoxin. | endotoxin prepared from several bordetella pertussis strains in both immunological phases i and iv gave two lipopolysaccharide peaks (lps-i and lps-ii) when analyzed on hydroxylapatite columns in a phosphate buffer containing 0.1% sodium dodecyl sulfate; these lipopolysaccharides, present in the ratio of 2:3, are true endotoxins by both chemical and biological criteria. endotoxins isolated from escherichia coli, salmonella typhimurium, and shigella flexneri gave single lipopolysaccharide peaks w ... | 1980 | 6249793 |
| neutron irradiation of bacteria in the presence and absence of secondary charged-particle equilibrium. | 1980 | 6251509 | |
| [isolation and characteristics of polymyxin-resistant mutants of shigella flexneri]. | some characteristics of s. flexneri 2a mutants resistant to various concentrations of polymyxin m were studied. the data indicate that mutations resulting in low (50 microgram/ml) and high (300 microgram/ml) levels of the antibiotic resistance were determined by different genes. polymyxin resistance led to changed permeability of the outer membrane with respect to detergents and some antibiotics, such as aminoglycosides, penicillins, chloramphenicol and amphotericin b but did not change sensitiv ... | 1980 | 6252826 |
| [effect of mutations imparting resistance to antibiotics having different mechanisms of action on the virulence of shigella flexneri]. | the study of the interaction between mutants of sh. flexneri 2a and epithelial hela cells has revealed that in all cases the loss of virulence in the studied groups of mutants, resistant to antibiotics with different mechanisms of action (polymyxin m, mecillinam and neamin) was linked with the loss of the ability of bacteria to penetrate into epithelial cells. these changes are probably the result of disturbances in the structure of external membrane in polymyxin-resistant mutants, in the regula ... | 1981 | 6269334 |
| lipopolysaccharide receptor on rabbit peritoneal macrophages. i. binding characteristics. | the bordetella pertussis endotoxin, labeled with tritium ((3h)-lps), bound irreversibly and nonspecifically to rabbit lung macrophages, but bound reversibly and specifically to both resident and elicited rabbit peritoneal macrophages. the specific binding capacity of the macrophages was saturated with about 3 x 10(4) lps molecules per cell. the binding was inhibited with the homologous unlabeled endotoxin, but not at all with endotoxin from proteus mirabilis, thus assessing ligand specificity. e ... | 1982 | 6278022 |
| involvement of a plasmid in the invasive ability of shigella flexneri. | representative shigella flexneri strains were studied to determine whether plasmids are involved in their virulence. all invasive s. flexneri strains, irrespective of serotype, were found to harbor a large plasmid of approximately 140 megadaltons in size, although some strains carried additional plasmid species. spontaneous variants of strains of serotypes 1, 2, and 5 had lost this 140-megadalton plasmid and had concomitantly become avirulent, i.e., could neither invade hela cell monolayers nor ... | 1982 | 6279518 |
| attachment and ingestion of bacteria by trophozoites of entamoeba histolytica. | entamoeba histolytica trophozoites were found to be very selective in their interactions with bacteria. two principal mechanisms were shown to be responsible for these interactions. certain bacteria, such as a number of escherichia coli and serratia marcescens strains which are known to contain mannose-binding components on their cell surface, bound to mannose receptors on the amoeba membrane. this attachment was markedly inhibited by alpha-methylmannoside (0.5%), especially when the incubations ... | 1982 | 6281192 |
| degradation of intestinal glycoproteins by pathogenic shigella flexneri. | intestinal mucin glycoproteins were examined for their ability to sustain growth of pathogenic shigella. inoculation of germfree cecal mucin glycoproteins with shigella flexneri 4b resulted at 48 h in a 940-fold increase in the enteropathogen concentration. investigation in vitro of enzymatic degradation by the pathogen led to the identification of a blood group b-degrading glycosidase produced by the bacteria. in in vivo experiments, fecal supernatants of mice monocontaminated with s. flexneri ... | 1982 | 6282754 |