Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| prevalence of avian influenza viruses, borrelia garinii, mycobacterium avium, and mycobacterium avium subsp. paratuberculosis in waterfowl and terrestrial birds in slovakia, 2006. | the prevalence of borrelia, mycobacteria and avian influenza virus (aiv) infections, together with the distribution of different aiv subtypes, was studied in migratory waterfowl and terrestrial birds trapped in three localities in slovakia during 2006. samples obtained from waterfowl captured in the senianske ponds area of eastern slovakia showed the highest diversity of aiv isolates. a total of 13 different subtypes were detected in 19 samples from this location (h1n2, h2n2, h3n2, h6n6, h7n6, h ... | 2008 | 18798030 |
| two-step synthesis of the immunogenic bacterial glycolipid bbgl1. | chemical synthesis of a bacterial glycolipid bbgl1 is reported in two steps starting from per-o-tms d-galactose. the key features are glycosyl iodide mediated beta-stereoselective glycosylation in the absence of neighboring group participation and regioselective acylation. | 2008 | 18798644 |
| retraction. localization of bmpa on the exposed outer membrane of borrelia burgdorferi by monospecific anti-recombinant bmpa rabbit antibodies. | 2008 | 18799811 | |
| skin homing of treponema pallidum in early syphilis: an immunohistochemical study. | the incidence of syphilis is increasing in most parts of the world including some major european cities. the lesions developing during secondary syphilis may be difficult to diagnose clinically. similarly, the histopathologic changes do not always fulfill the typical diagnostic criteria. the objective of this study was to assess the contribution of immunohistochemistry for the identification and localization of treponema pallidum on the skin. we retrieved from our files 12 paraffin-embedded biop ... | 2009 | 18800002 |
| interleukin 10 protects the brain microcirculation from spirochetal injury. | spirochetal infections are an important cause of neurological disease. in previous studies of the pathogenesis of spirochetal brain infection, mice inoculated with borrelia turicatae, an agent of tick-borne relapsing fever in north america, developed mild meningitis and parenchymal activation/infiltration by interleukin 10 (il-10)-producing microglia/macrophages. here, we investigated the neuroprotective effects of il-10 during spirochetal infection by comparing the outcomes of b. turicatae infe ... | 2008 | 18800010 |
| detection of tick-borne pathogens by masstag polymerase chain reaction. | masstag polymerase chain reaction (pcr) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. after multiplex pcr, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. the identification of a microbe in a sample is determined by its cognate tags. here we describe establishment and implementation of a masstag pcr panel for surveillance of microbes implicated in tick-vectored infectious d ... | 2009 | 18800864 |
| nod2 plays an important role in the inflammatory responses of microglia and astrocytes to bacterial cns pathogens. | while glial cells are recognized for their roles in maintaining neuronal function, there is growing appreciation that resident central nervous system (cns) cells initiate and/or augment inflammation following trauma or infection. we have recently demonstrated that microglia and astrocytes constitutively express nucleotide-binding oligomerization domain-2 (nod2), a member of the novel nucleotide-binding domain leucine-rich repeat region containing a family of proteins (nlr) that functions as an i ... | 2009 | 18803303 |
| borrelia burgdorferi lacking dbpba exhibits an early survival defect during experimental infection. | several borrelia burgdorferi genes induced under mammalian host conditions have been purported to be important in lyme disease pathogenesis based on their binding to host structures. these genes include the dbpba locus, whose products bind host decorin and glycosoaminoglycans. recently, the dbpba genes were reported to be involved in borrelial infectivity. here we extended the previous observations by using culture and quantitative pcr to evaluate low- and high-dose murine infection by a delta d ... | 2008 | 18809667 |
| cerebral vasculitis and stroke in lyme neuroborreliosis. two case reports and review of current knowledge. | we report on 2 patients with cerebral vasculitis and stroke due to lyme neuroborreliosis (lnb). both patients had a prodromal stage involving headaches, and showed meningeal enhancement in addition to ischemic infarctions on brain magnetic resonance imaging and diffuse vasculitis on vascular imaging. serological and cerebrospinal (csf) fluid studies confirmed the diagnosis of active lnb. ceftriaxone for 3 weeks led to an excellent recovery and improvements in the csf examination findings. stroke ... | 2008 | 18810231 |
| borrelia burgdorferi sensu lato, the agent of lyme borreliosis: life in the wilds. | in europe, borrelia burgdorferi sensu lato (sl) the agent of lyme borreliosis circulates in endemic areas between ixodes ricinus ticks and a large number of vertebrate hosts upon which ticks feed. currently, at least 12 different borrelia species belonging to the complex b. burgdorferi sl have been identified among which seven have been detected in i. ricinus: b. burgdorferi sensu stricto (ss), b. garinii, b. afzelii, b. valaisiana, b. spielmanii and b. bissettii. a few dozens of vertebrate host ... | 2008 | 18814688 |
| persisting atypical and cystic forms of borrelia burgdorferi and local inflammation in lyme neuroborreliosis. | the long latent stage seen in syphilis, followed by chronic central nervous system infection and inflammation, can be explained by the persistence of atypical cystic and granular forms of treponema pallidum. we investigated whether a similar situation may occur in lyme neuroborreliosis. | 2008 | 18817547 |
| primary cutaneous marginal zone b-cell lymphoma: a molecular and clinicopathological study of cases from asia, germany, and the united states. | primary cutaneous marginal zone b-cell lymphoma is considered the cutaneous counterpart of extranodal marginal zone b-cell lymphoma of mucosa-associated lymphoid tissue. although its molecular pathogenesis is currently unknown, an etiological link with borrelia burgdorferi infection has been identified in european, but not in american or asian cases. to better understand the pathogenesis and the geographical differences of cutaneous marginal zone b-cell lymphoma, 60 cases from the east asia, ger ... | 2008 | 18820662 |
| frequency of antibodies to babesia bigemina, b. bovis, anaplasma marginale, trypanosoma vivax and borrelia burgdorferi in cattle from the northeastern region of the state of pará, brazil. | babesiosis, anaplasmosis, and trypanosomosis are relevant diseases, potentially causing morbidity in cattle, leading to economic losses. borreliosis is import as a potential zoonosis. the objective of this study was to determine, by indirect enzyme-linked immunosorbent assay (elisa), the frequency of seropositive cattle to babesia bigemina, b. bovis, anaplasma marginale, trypanosoma vivax and borrelia burgdorferi in cattle from the northeastern region of pará, brazil. sera samples from 246 femal ... | 2008 | 18823579 |
| adenoviral delivery of interleukin-10 fails to attenuate experimental lyme disease. | production of interleukin-10 (il-10) by c57bl/6 mice following infection with borrelia burgdorferi has been proposed as a mechanism whereby resistance to the development of experimental lyme arthritis is maintained. in the current study, we sought to determine the role of il-10 during infection of arthritis- and carditis-susceptible c3h mice. infection of c3h il-10(-/-) mice led to increased joint swelling and arthritis severity scores over those of wild-type c3h mice. measurement of b. burgdorf ... | 2008 | 18824530 |
| deciphering the ligand-binding sites in the borrelia burgdorferi complement regulator-acquiring surface protein 2 required for interactions with the human immune regulators factor h and factor h-like protein 1. | borrelia burgdorferi, the etiologic agent of lyme disease, employs sophisticated means to evade killing by its mammalian hosts. one important immune escape mechanism is the inhibition of complement activation mediated by interactions of the host-derived immune regulators factor h (cfh) and factor h-like protein 1 (cfhl1) with borrelial complement regulator-acquiring surface proteins (bbcrasps). bbcrasp-2 is a distinctive cfh- and cfhl1-binding protein that is produced by serum-resistant b. burgd ... | 2008 | 18824548 |
| [small risk of developing lyme borreliosis following a tick bite on ameland: research in a general practice]. | to investigate the percentage of ticks infected with borrelia burgdorferi on the dutch north sea island of ameland, and the risk of developing lyme disease following tick bite on the island. | 2008 | 18825891 |
| [characteristic features of the ranks genus and subgenus, and an intercalary rank "species complex" in ixodid ticks (acari: ixodidae)]. | the work was carries our from the standpoint of the morphological conception of species. vast collections of the zoological institute of the russian academy of science testify to the existence of hiatuses in both genera and subgenera of palearctic ixodids at all active phases of their ontogenesis. the fact that the subgenera of palearctic genera have been well studies is notes, and composition of the subgenera is presented. the question of a taxonomic intercalary rank "species complex" is consid ... | 2008 | 18825916 |
| internal core protein cleavage leaves the hepatitis b virus capsid intact and enhances its capacity for surface display of heterologous whole chain proteins. | virus capsids find increasing use as nanoparticulate platforms for the surface display of heterologous ligands, including as multivalent vaccine carriers. presentation on the icosahedral hepatitis b virus capsid (hbcag) is known to strongly enhance immunogenicity of foreign sequences, most efficiently if they are inserted into the dominant c/e1 b cell epitope, a surface-exposed loop in the center of the constituent core protein primary sequence. even some complete proteins were successfully inse ... | 2008 | 18826949 |
| then and now: use of 16s rdna gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. | in the last decade, as a result of the widespread use of pcr and dna sequencing, 16s rdna sequencing has played a pivotal role in the accurate identification of bacterial isolates and the discovery of novel bacteria in clinical microbiology laboratories. for bacterial identification, 16s rdna sequencing is particularly important in the case of bacteria with unusual phenotypic profiles, rare bacteria, slow-growing bacteria, uncultivable bacteria and culture-negative infections. not only has it pr ... | 2008 | 18828852 |
| surveillance for lyme disease--united states, 1992-2006. | lyme disease is a multisystem disease that occurs in north america, europe, and asia. in the united states, the etiologic agent is borrelia burgdorferi sensu stricto, a spirochete transmitted to humans by infected ixodes scapularis and i. pacificus ticks. the majority of patients with lyme disease develop a characteristic rash, erythema migrans (em), accompanied by symptoms of fever, malaise, fatigue, headache, myalgia, or arthralgia. other manifestations of infection can include arthritis, card ... | 2008 | 18830214 |
| interaction of the lyme disease spirochete borrelia burgdorferi with brain parenchyma elicits inflammatory mediators from glial cells as well as glial and neuronal apoptosis. | lyme neuroborreliosis, caused by the spirochete borrelia burgdorferi, often manifests by causing neurocognitive deficits. as a possible mechanism for lyme neuroborreliosis, we hypothesized that b. burgdorferi induces the production of inflammatory mediators in the central nervous system with concomitant neuronal and/or glial apoptosis. to test our hypothesis, we constructed an ex vivo model that consisted of freshly collected slices from brain cortex of a rhesus macaque and allowed live b. burgd ... | 2008 | 18832582 |
| molecular mechanisms involved in vascular interactions of the lyme disease pathogen in a living host. | hematogenous dissemination is important for infection by many bacterial pathogens, but is poorly understood because of the inability to directly observe this process in living hosts at the single cell level. all disseminating pathogens must tether to the host endothelium despite significant shear forces caused by blood flow. however, the molecules that mediate tethering interactions have not been identified for any bacterial pathogen except e. coli, which tethers to host cells via a specialized ... | 2008 | 18833295 |
| common and unique contributions of decorin-binding proteins a and b to the overall virulence of borrelia burgdorferi. | as an extracellular bacterium, the lyme disease spirochete borrelia burgdorferi resides primarily in the extracellular matrix and connective tissues and between host cells during mammalian infection, where decorin and glycosaminoglycans are abundantly found, so its interactions with these host ligands potentially affect various aspects of infection. decorin-binding proteins (dbps) a and b, encoded by a 2-gene operon, are outer surface lipoproteins with similar molecular weights and share approxi ... | 2008 | 18833332 |
| variations in ixodes ricinus density and borrelia infections associated with cattle introduced into a woodland in the netherlands. | the effect of introduced large herbivores on the abundance of ixodes ricinus ticks and their borrelia infections was studied in a natural woodland in the netherlands. oak and pine plots, either ungrazed or grazed by cattle, were selected. ticks were collected weekly by blanket dragging. borrelia infections were determined by pcr and restriction fragment length polymorphism. rodent densities were estimated using mark-release-recapture methods. on occasion, the cattle were inspected for tick infes ... | 2008 | 18836006 |
| identifying the reservoir hosts of the lyme disease spirochete borrelia burgdorferi in california: the role of the western gray squirrel (sciurus griseus). | we investigated the role of the western gray squirrel (sciurus griseus) as a reservoir host of the lyme disease spirochete borrelia burgdorferi. a survey of 222 western gray squirrels in california showed an overall prevalence of b. burgdorferi infection of 30%, although at a county level, prevalence of infection ranged from 0% to 50% by polymerase chain reaction. laboratory trials with wild-caught western gray squirrels indicated that squirrels were competent reservoir hosts of the lyme disease ... | 2008 | 18840740 |
| the promiscuity of beta-strand pairing allows for rational design of beta-sheet face inversion. | recent studies suggest the dominant role of main-chain h-bond formation in specifying beta-sheet topology. its essentially sequence-independent nature implies a large degree of freedom in designing beta-sheet-based nanomaterials. here we show rational design of beta-sheet face inversions by incremental deletions of beta-strands from the single-layer beta-sheet of borrelia outer surface protein a. we show that a beta-sheet structure can be maintained when a large number of native contacts are rem ... | 2008 | 18842042 |
| cross-reaction between yersinia outer membrane proteins and anti-borrelia antibodies in sera of patients with lyme disease. | diagnosis of yersinia infections accompanied by reactive arthritis could be complicated by cross-reaction with other arthritogenic bacteria. the possible cross-reaction between yersinia antigens and anti-borrelia antibodies in blood sera of patients with lyme disease was studied. the occurrence of specific iga, igg and igm antibodies was analyzed in serum samples from 30 patients with yersinia-triggered reactive arthritis, 30 patients with lyme disease and five samples from healthy blood donors. ... | 2008 | 18844689 |
| borrelia burgdorferi expression of the bba64, bba65, bba66, and bba73 genes in tissues during persistent infection in mice. | borrelia burgdorferi, the etiological agent of lyme disease in humans, is vectored between mammalian hosts in nature by ixodes ticks. the organism adapts to diverse environments encountered throughout the enzootic cycle by differentially expressing essential gene products to survive the specialized conditions, whether in ticks or warm-blooded hosts. however, little is known regarding the identity and/or function of b. burgdorferi genes expressed during colonization of tissues during mammalian in ... | 2008 | 18848981 |
| in vitro cpg methylation increases the transformation efficiency of borrelia burgdorferi strains harboring the endogenous linear plasmid lp56. | borrelia burgdorferi is the causative agent of lyme disease, the most common vector-borne illness in the northern hemisphere. low-passage-number infectious strains of b. burgdorferi exhibit extremely low transformation efficiencies-so low, in fact, as to hinder the genetic study of putative virulence factors. two putative restriction-modification (r-m) systems, bbe02 contained on linear plasmid 25 (lp25) and bbq67 contained on lp56, have been postulated to contribute to this poor transformabilit ... | 2008 | 18849429 |
| association of urine protein excretion and infection with borrelia burgdorferi sensu lato in bernese mountain dogs. | bernese mountain dogs (bmds) are prone to develop a familial glomerulonephropathy and a pathogenic role of borrelia burgdorferi sensu lato in this disease has been suspected. glomerular disease in many affected dogs is clinically inapparent and proteinuria is found incidentally. in this study, urine protein excretion was evaluated in 122 clinically healthy bmds and 55 controls. the seroprevalence of b. burgdorferi in bmds was 57%, compared to 16% in controls. there were no significant difference ... | 2009 | 18930416 |
| clinical predictors of lyme disease among children with a peripheral facial palsy at an emergency department in a lyme disease-endemic area. | although lyme disease can cause peripheral facial palsy in lyme disease-endemic areas, diagnostic predictors in children have not been described. | 2008 | 18931349 |
| isolation of borrelia burgdorferi sensu lato from the skin of the european badger (meles meles) in switzerland. | no data are available on the role of badgers in the ecology of lyme borreliosis spirochetes in europe. in a recent study describing validation of a molecular method allowing host dna identification and borrelia burgdorferi sensu lato detection in ixodes ricinus, the simultaneous presence of b. afzelii dna and of european badger (meles meles) dna was detected in i. ricinus ticks in switzerland. this suggested that badgers might be reservoir hosts for b. afzelii. here, we present results obtained ... | 2009 | 18945190 |
| evaluation of the recombinant vlse-based liaison chemiluminescence immunoassay for detection of borrelia burgdorferi and diagnosis of lyme disease. | recent efforts to improve the serologic diagnosis of lyme disease have included the use of a synthetic peptide (c6) that reproduces the sequence of invariable region 6 of vlse, the variable surface antigen of borrelia burgdorferi. in the present study, the diagnostic performance of diasorin's recombinant vlse-based chemiluminescence immunoassay in 1,947 human serum samples was evaluated. sensitivity was determined using two serum panels from the cdc. for panel i, we observed sensitivities of 68. ... | 2008 | 18945880 |
| retrospective clinical and molecular analysis of conditioned laboratory dogs (canis familiaris) with serologic reactions to ehrlichia canis, borrelia burgdorferi, and rickettsia rickettsii. | dogs are susceptible to different tickborne infections, including members of the anaplasmataceae (ehrlichia canis, e. ewingii, e. chaffeensis, anaplasma phagocytophilum, a. platys), borrelia burgdorferi, and rickettsia rickettsii. these diseases can manifest with clinical signs including fever, anorexia, malaise, lameness, rash, and bleeding episodes; however, these signs are nonpathognomonic, and infections can occur in the absence of clinical signs. hematologic abnormalities can include leukop ... | 2008 | 18947166 |
| percent positive rate of lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue. | we performed a retrospective review of 23,777 specimens tested by our lyme real-time polymerase chain reaction assay to determine the percent positive rates by specimen source. the percent positive rates were highest in synovial fluid (6.4%) and tissue (6.5%), and lowest in blood (0.1%) and cerebrospinal fluid (0.09%). | 2008 | 18947959 |
| iram-an alpha2-macroglobulin from the hard tick ixodes ricinus: characterization and function in phagocytosis of a potential pathogen chryseobacterium indologenes. | the universal protease inhibitors of the alpha(2)-macroglobulin (alpha(2)m) family are evolutionarily conserved constituents of innate immunity, presumably because they guard organisms against undesired proteolytic attacks of a different origin. here, we determined the primary structure of alpha(2)-macroglobulin from the hard tick ixodes ricinus (iram) by sequencing of overlapping pcr products. predicted disulfide and glycosylation patterns, post-translational cleavage and alternative splicing w ... | 2009 | 18948134 |
| modification of borrelia burgdorferi to overproduce ospa or vlse alters its infectious behaviour. | the surface lipoproteins of the lyme disease spirochaete borrelia burgdorferi directly interact with tissue microenvironments during mammalian infection, and thus potentially affect various aspects of infection. to investigate the influence of surface antigen synthesis on infectious behaviour, b. burgdorferi was modified to constitutively produce the well-characterized surface lipoproteins ospa and invariant vlse. although increasing ospa or vlse production did not significantly affect synthesis ... | 2008 | 18957595 |
| severity of lyme disease with persistent symptoms. insights from a double-blind placebo-controlled clinical trial. | lyme disease is a global health concern and is the world's leading tick borne infection caused by the spirochete, borrelia burgdorferi, that has been associated with numerous neurologic, rheumatologic and psychiatric manifestations. the symptoms of lyme disease have been characterized as either severe or ''related to the aches and pains of daily living.'' a randomized double-blind, placebo-controlled clinical trial (rct) was conducted in a primary internal medicine practice in westchester county ... | 2008 | 18971914 |
| skin manifestations of lyme borreliosis: diagnosis and management. | lyme borreliosis is a multisystem infectious disease caused by tick-transmitted spirochetes of the borrelia burgdorferi sensu lato complex. the three characteristic cutaneous manifestations are erythema migrans, borrelial lymphocytoma, and acrodermatitis chronica atrophicans. erythema migrans occurs in acute lyme borreliosis, lymphocytoma is a subacute lesion, and acrodermatitis is the typical manifestation of late lyme borreliosis. clinical appearances of erythema migrans and lymphocytoma (when ... | 2008 | 18973402 |
| serologic survey of the wild boar (sus scrofa) for borrelia burgdorferi sensu lato. | sera of 642 wild boars (sus scrofa) shot by hunters in ten administrative regions of the czech republic during 1995-2000, were tested by indirect hemagglutination assay (iha) for the presence of anti-borrelia igg. antibodies to borrelia burgdorferi sensu lato (bb) were detected in serum samples from all 10 regions, and overall seroprevalence rate was 12.8%. titres of antibodies ranged from 1:80 to 1:640. borrelia antibodies were most frequent in the animals from three administrative regions of t ... | 2009 | 18973452 |
| borrelia burgdorferi napa-driven th17 cell inflammation in lyme arthritis. | human lyme arthritis caused by borrelia burgdorferi is characterized by an inflammatory infiltrate that consists mainly of neutrophils and t cells. this study was undertaken to evaluate the role of the innate and acquired immune responses elicited by the neutrophil-activating protein a (napa) of b burgdorferi in patients with lyme arthritis. | 2008 | 18975343 |
| [neuroborreliosis in north jutland]. | infection with borrelia sp. is common in denmark, and dissemination to the central nervous system (neuroborreliosis) may develop in a minority of cases with varying symptoms. we here present a population-based review of neuroborreliosis in northern denmark. | 2008 | 18976599 |
| profiling the humoral immune response to borrelia burgdorferi infection with protein microarrays. | to determine the cell envelope proteins of borrelia burgdorferi recognized by immune sera of patients with late lyme disease, we developed a borrelia microarray containing proteins encoded by 90 cell envelope genes and their homologs described in the annotated genomic sequence of b. burgdorferi, strain b31. the protein microarray was used to profile the humoral immune response using sera from 13 patients with late lyme disease and four normal controls. although there was considerable heterogenei ... | 2008 | 18976702 |
| fever in a 7-year-old girl returning from mali. | 2008 | 18986264 | |
| examination of specific dna by pcr in patients with different forms of lyme borreliosis. | borrelial specific dna was examined in a group of 62 patients with different forms of lyme borreliosis (lb) (32 patients suffered from neuroborreliosis, 19 manifested erythema migrans, and 11 joint involvement). | 2008 | 18986344 |
| the chronic debate over lyme disease. | 2008 | 18989271 | |
| borrelia burgdorferi infection-associated surface proteins erpp, erpa, and erpc bind human plasminogen. | host-derived plasmin plays a critical role in mammalian infection by borrelia burgdorferi. the lyme disease spirochete expresses several plasminogen-binding proteins. bound plasminogen is converted to the serine protease plasmin and thereby may facilitate the bacterium's dissemination throughout the host by degrading extracellular matrix. in this work, we demonstrate plasminogen binding by three highly similar borrelial outer surface proteins, erpp, erpa, and erpc, all of which are expressed dur ... | 2009 | 19001079 |
| effect of passive immunoglobulin transfer on results of diagnostic tests for antibodies against borrelia burgdorferi in pups born to a seropositive dam. | the event that 8 of 12 pups born to a lyme borreliosis-positive dam tested positive on a commonly used in-hospital lyme borreliosis test kit at 1 week of age prompted breeder concern about the possibility and implications of transplacental lyme borreliosis infection. almost 2 weeks after the initial serologic test results were obtained, blood was collected from the puppies for comprehensive testing. assessment of the findings indicate the possibility that passive transfer of maternally derived a ... | 2008 | 19003779 |
| [what is the value of a rapid tick test for borrelia (interview by dr. judith neumaier)]. | 2008 | 19006886 | |
| lyme neuroborreliosis in children: a prospective study of clinical features, prognosis, and outcome. | evaluation of children with clinically suspected neuroborreliosis (nb) is difficult. with a prospective study design we wanted to characterize children with signs and symptoms indicative for nb, investigate clinical outcome and, if possible, identify factors of importance for recovery. | 2008 | 19008771 |
| does my genome look big in this? | 2008 | 19008888 | |
| the flat-ribbon configuration of the periplasmic flagella of borrelia burgdorferi and its relationship to motility and morphology. | electron cryotomography was used to analyze the structure of the lyme disease spirochete, borrelia burgdorferi. this methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. using electron cryotomography, we noted that membrane blebs formed at the ends of the cells. these blebs may be precursors to vesicles that are released from cells grown in vivo and in vitro. we fou ... | 2009 | 19011030 |
| extensive bullous lichen sclerosus with scarring alopecia. | we describe details of a very rare variant of lichen sclerosus in an elderly man. this pattern was characterized by involvement of most of the body surface by bullae, erosions and scarring, including scarring alopecia. our patient responded to topical corticosteroids and doxycycline. borrelia spp. have been implicated in a previous case of this variant, but we could not find any evidence of this in our patient. | 2009 | 19018788 |
| abrogation of ospab constitutively activates the rrp2-rpon-rpos pathway (sigman-sigmas cascade) in borrelia burgdorferi. | molecular mechanisms underlying the reciprocal regulation of the two major surface lipoproteins and virulence factors of borrelia burgdorferi, ospa and ospc, are not fully understood. herein, we report that inactivation of the ospab operon resulted in overproduction of ospc and many other lipoproteins via the constitutive activation of the rrp2-rpon-rpos pathway. complementing the ospab mutant with a wild-type copy of ospa, but not an ospa variant that lacks the lipoprotein signal sequence, rest ... | 2008 | 19019147 |
| borrelia carolinensis sp. nov., a new (14th) member of the borrelia burgdorferi sensu lato complex from the southeastern region of the united states. | approximately 118 borrelia isolates were cultured from a variety of rodents, birds, and ticks collected in the southern united states. in addition to a highly diverse group of borrelia bissettii strains and a homogenous group of borrelia burgdorferi sensu stricto strains, a group of 16 isolates with unusual characteristics was found. the isolates were cultured from ear biopsy samples of the rodents peromyscus gossypinus and neotoma floridana trapped at five localities in south carolina. a multil ... | 2009 | 19020062 |
| "borrelia-associated early-onset morphea": a particular type of scleroderma in childhood and adolescence with high titer antinuclear antibodies? results of a cohort analysis and presentation of three cases. | morphea is an inflammatory autoimmune skin sclerosis of unknown etiology. a causative role of borrelia burgdorferi infection has been controversially discussed, but no conclusive solution has yet been achieved. | 2009 | 19022534 |
| the bicarbonate transporter is essential for bacillus anthracis lethality. | in the pathogenic bacterium bacillus anthracis, virulence requires induced expression of the anthrax toxin and capsule genes. elevated co2/bicarbonate levels, an indicator of the host environment, provide a signal ex vivo to increase expression of virulence factors, but the mechanism underlying induction and its relevance in vivo are unknown. we identified a previously uncharacterized abc transporter (bas2714-12) similar to bicarbonate transporters in photosynthetic cyanobacteria, which is essen ... | 2008 | 19023421 |
| transcription and genetic analyses of a putative n-acetylmuramyl-l-alanine amidase in borrelia burgdorferi. | in this study, a putative n-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the lyme disease spirochete borrelia burgdorferi. this protein shares c. 30% identity with its counterparts from other bacteria. reverse transcriptase-pcr analysis showed that bb0666 along with two other genes (bb0665 and bb0667) are cotranscribed with the motility and chemotaxis genes. this newly identified operon is termed as pami. sequence and primer extension analyses showed that pami wa ... | 2009 | 19025570 |
| role of psychiatric comorbidity in chronic lyme disease. | to evaluate the prevalence and role of psychiatric comorbidity and other psychological factors in patients with chronic lyme disease (cld). | 2008 | 19035409 |
| persistent arthritis in borrelia burgdorferi-infected hla-dr4-positive cd28-negative mice post-antibiotic treatment. | the immunologic events that lead to persistent joint inflammation in certain patients with lyme arthritis post-antibiotic treatment have been elusive so far. the prevalence of this condition is highest in individuals with rheumatoid arthritis-associated hla-dr alleles. this study was undertaken to generate a murine model with persistent arthritis post-antibiotic treatment. | 2008 | 19035513 |
| soda is essential for virulence of borrelia burgdorferi in the murine model of lyme disease. | borrelia burgdorferi, the causative agent of lyme disease, has a limited set of genes to combat oxidative/nitrosative stress encountered in its tick vector or mammalian hosts. we inactivated the gene encoding for superoxide dismutase a (soda, bb0153), an enzyme mediating the dismutation of superoxide anions and examined the in vitro and in vivo phenotype of the mutant. there were no significant differences in the in vitro growth characteristics of the soda mutant compared with the control strain ... | 2009 | 19040638 |
| a previously healthy 11-year-old girl with behavioural disturbances, desquamation of the skin and loss of teeth. | an 11-year-old girl was admitted with backpain, weight loss, fatigue and behavioural disturbances, starting seven weeks before admission. physical examination showed acrodynia, tremor, cachexia, hypertension and extensive gingival ulceration. routine laboratory tests were normal, except for a crp of 98 mg/l. screening tests for recreational drugs as well as antibody assays for hiv, hepatitis b and borrelia burgdorferia were negative. chest x-ray, brain cat and mri scan were all normal. lumbar pu ... | 2009 | 19043736 |
| a critical role for type i ifn in arthritis development following borrelia burgdorferi infection of mice. | gene expression analysis previously revealed a robust ifn-responsive gene induction profile that was selectively up-regulated in borrelia burgdorferi-infected c3h mice at 1 wk postinfection. this profile was correlated with arthritis development, as it was absent from infected, mildly arthritic c57bl/6 mice. in this report we now demonstrate that profile induction in infected c3h scid mice occurs independently of b or t lymphocyte infiltration in the joint tissue. additionally, type i ifn recept ... | 2008 | 19050267 |
| bacterin that induces anti-ospa and anti-ospc borreliacidal antibodies provides a high level of protection against canine lyme disease. | groups of 15 laboratory-bred beagles were vaccinated and boosted with either a placebo or adjuvanted bivalent bacterin comprised of a traditional borrelia burgdorferi strain and a unique ospa- and ospb-negative b. burgdorferi strain that expressed high levels of ospc and then challenged with b. burgdorferi-infected ixodes scapularis ticks. the vaccinated dogs produced high titers of anti-ospa and anti-ospc borreliacidal antibodies, including borreliacidal antibodies specific for an epitope withi ... | 2009 | 19052162 |
| [tick-borne disease or not? do not let yourself be fooled]. | 2008 | 19058392 | |
| differential role of passerine birds in distribution of borrelia spirochetes, based on data from ticks collected from birds during the postbreeding migration period in central europe. | borrelia spirochetes in bird-feeding ticks were studied in the czech republic. during the postbreeding period (july to september 2005), 1,080 passerine birds infested by 2,240 ixodes ricinus subadult ticks were examined. borrelia garinii was detected in 22.2% of the ticks, borrelia valaisiana was detected in 12.8% of the ticks, borrelia afzelii was detected in 1.6% of the ticks, and borrelia burgdorferi sensu stricto was detected in 0.3% of the ticks. after analysis of infections in which the bl ... | 2009 | 19060160 |
| nkt cells prevent chronic joint inflammation after infection with borrelia burgdorferi. | borrelia burgdorferi is the etiologic agent of lyme disease, a multisystem inflammatory disorder that principally targets the skin, joints, heart, and nervous system. the role of t lymphocytes in the development of chronic inflammation resulting from b. burgdorferi infection has been controversial. we previously showed that natural killer t (nkt) cells with an invariant (i) tcr alpha chain (inkt cells) recognize glycolipids from b. burgdorferi, but did not establish an in vivo role for inkt cell ... | 2008 | 19060201 |
| ribotyping and whole-cell protein analysis of spirochetes isolated from arthropods in the czech republic. | in the presented work, six borrelia burgdorferi sensu lato isolates were recovered from ixodes ricinus ticks and one strain of spirochete with uncertain taxonomic position isolated from culex pipiens mosquito collected in the valtice area (south moravia, czech republic), as well as representative type strains, were further characterized by ribotyping and whole-cell protein fingerprinting. the ribotype patterns obtained with hindii and ecorv ribotype patterns showed good correlations with the rec ... | 2008 | 19061259 |
| study on lyme borreliosis focus in the lublin region (eastern poland). | a suburban focus of lyme borreliosis situated 11 km from the southern border of the city of lublin (eastern poland) was characterized. the focus covers an area of circa 100 km(2), surrounding 3 localities inhabited by circa 7,500 people engaged mostly in farming. it was demonstrated that on the area of focus the infection rate of ixodes ricinus ticks with borrelia burgdorferi, frequency of serological response of inhabitants to the antigen of borrelia burgdorferi, and incidence of lyme borrelios ... | 2008 | 19061271 |
| clinical characteristics of childhood lyme neuroborreliosis in an endemic area of northern europe. | neuroborreliosis may be caused by different species of borrelia burgdorferi (bb) and the clinical presentation of neuroborreliosis in children may differ between geographical areas due to occurrence of different bb genospecies. the aim of this study was to evaluate the clinical characteristics in children with neuroborreliosis in an endemic area of scandinavia. during 1996-2006, children with suspected neuroborreliosis referred to stavanger university hospital were investigated by a standard pro ... | 2009 | 19065451 |
| chronic lyme disease and the 'axis of evil'. | lyme disease is a controversial illness, and the existence of chronic lyme disease induced by persistent infection with the lyme spirochete, borrelia burgdorferi, is the subject of continued debate. a recent publication defined the 'axis of evil' in this controversy as physicians who treat patients with needlessly prolonged courses of antibiotics, 'specialty laboratories' that perform 'inaccurate' lyme testing and the internet, which promotes 'lyme hysteria'. we examine the 'axis of evil' compon ... | 2008 | 19072179 |
| in vitro susceptibility of borrelia spielmanii to antimicrobial agents commonly used for treatment of lyme disease. | ten isolates of the recently delineated genospecies borrelia spielmanii were tested against antimicrobial agents used to treat lyme disease and compared to eight isolates of the other three human-pathogenic borrelial genospecies. despite some small but significant differences in four out of eight antibiotic agents, the susceptibility pattern of b. spielmanii mainly parallels that of the other known human-pathogenic members of the b. burgdorferi sensu lato complex. | 2009 | 19075048 |
| borrelia burgdorferi is not associated with genital lichen sclerosus in men. | 2009 | 19077073 | |
| [borrelial erythema of the face]. | borrelial infection is characterized by various skin manifestations that are usually classified into three main types: chronic migratory erythema, borrelial lymphocytoma and acrodermatitis chronica atrophicans. we report an unusual case of borrelial cutaneous infection presenting as a mediofacial erythema that cannot be included in any of these three categories. | 2008 | 19084697 |
| a novel and simple method for laboratory diagnosis of relapsing fever borreliosis. | relapsing fever caused by borrelia bacteria is often obscured by malaria and incorrectly treated. here a novel method for diagnosis is presented. the method is cheap, simple and requires minimal laboratory material. despite its simplicity, the method shows surprisingly high sensitivity, detecting concentrations less than 10 bacteria/ml blood. | 2008 | 19088905 |
| [reversible complete heart block by re-infection with borrelia burgdorferi with negative igm-antibodies]. | past history and physical examination: a 38-year-old farmer presented at his general practitioner with dizziness. physical examination was notable for a heart rate of 35 beats/min. the electrocardiogram (ecg) showed a complete (third degree) heart block with a bradycardic ventricular escape rhythm. the patient reported having had an rash on his right lower leg six weeks previously. after spreading centrifugally it had turned pale in its centre, then regressed and finally disappeared. after havin ... | 2009 | 19090448 |
| lymphoma look-alike. | 2009 | 19092265 | |
| tick talk: unusually severe case of tick-borne relapsing fever with acute respiratory distress syndrome--case report and review of the literature. | borrelia hermsii causes tick-borne relapsing fever (tbrf) in the pacific northwest. there are few reports of tbrf-associated acute respiratory distress syndrome. one such unusually severe case is described. literature is reviewed including diagnosis, treatment, and prevention. | 2008 | 19099321 |
| fine-scale phylogeographic structure of borrelia lusitaniae revealed by multilocus sequence typing. | borrelia lusitaniae is an old world species of the lyme borreliosis (lb) group of tick-borne spirochetes and prevails mainly in countries around the mediterranean basin. lizards of the family lacertidae have been identified as reservoir hosts of b. lusitaniae. these reptiles are highly structured geographically, indicating limited migration. in order to examine whether host geographic structure shapes the evolution and epidemiology of b. lusitaniae, we analyzed the phylogeographic population str ... | 2008 | 19104655 |
| granuloma annulare displaying pseudorosettes in borelia infection. | in 2003, pseudorosettes were described as highly suspicious of infection by borrelia burgdorferi sensu lato in the appropriate clinical context. nevertheless, such a pattern has been described in the literature in other non-infectious conditions. on the other hand, granuloma annulare (ga) has been recently closely associated with infection by borrelia. we investigated how frequently pseudorosettes can be detected in common ga cases confirmed for borrelia by pcr. | 2008 | 19104742 |
| [failures of antibiotic treatment in lyme arthritis]. | antibiotic treatment has been proven to be effective in about 90% of patients with lyme arthritis in controlled studies. overt arthritis persisting in spite of antibiotic therapy is rare and most likely has an autoimmune background. more frequently, patients with history of lyme borreliosis present with non-specific articular and musculosceletal symptoms, which seem to be permanent sequelae of arthritis or constitute part of so called post-lyme disease syndrome, of unclear pathogenesis. as persi ... | 2008 | 19108522 |
| the loss and gain of marginal zone and peritoneal b cells is different in response to relapsing fever and lyme disease borrelia. | t cell-independent abs are protective against lyme disease and relapsing fever, illnesses caused by borrelia spirochetes with distinct blood-borne phases of infection. to understand this protective response, we characterized splenic and peritoneal b cell compartments during infection using flow cytometry and immunohistochemistry. in the spleen, early after infection, borrelia crocidurae, a relapsing fever species, induced a striking loss of marginal zone (mz) b cells from the mz, while borrelia ... | 2009 | 19109181 |
| [re-infection with borrelia burgdorferi s.l in a patient with a history of neuroborreliosis--case report]. | reinfection with borrelia burgdorferi s.l., which is likely in highly exposed persons, has not been described in poland so far. symptoms of lyme arthritis, preceded by typical skin lesion (erythema migrans) appeared in 46 years old women 5 years after successful treatment of borrelial meningitis. re-appearance of symptoms of lyme borreliosis following localized skin lesion, after a long asymptomatic period, as well as accompanying increase in specific antibodies, point to reinfection with borrel ... | 2008 | 19112843 |
| [reinfection in lyme borreliosis]. | persons exposed to tick bites in endemic areas of lyme borreliosis are at high risk of repeated infections with borrelia burgdorferi s.l. typically, recurrent episodes of lyme borreliosis present as erythema migrans, but systemic manifestations, like borrelial arthritis and meningitis, have been described. differentiating reinfection from relapse or chronic sequelae of primary infection should be possible on the basis of epidemiological data, clinical course and careful interpretation of serolog ... | 2008 | 19112844 |
| acute ischaemic pontine stroke revealing lyme neuroborreliosis in a young adult. | we report the case of a 23-year-old male patient who suddenly developed right hemiparesis, cerebellar ataxia, dysarthria, and bilateral dysmetria. brain magnetic resonance (mr) examination demonstrated hyperacute ischaemic lesions within the pons. csf analysis revealed a high protein content, lymphocytic pleocytosis, and oligoclonal igg bands not present in the serum. elevated igm and igg anti-borrelia burgdorferi antibodies were shown in both serum and csf samples, associated with an intratheca ... | 2008 | 19115674 |
| genome rhetoric and the emergence of compositional bias. | genomes exhibit diverse patterns of species-specific gc content, gc and at skews, codon bias, and mutation bias. despite intensive investigations and the rapid accumulation of sequence data, the causes of these a priori different genome biases have not been agreed on and seem multifactorial and idiosyncratic. we show that these biases can arise generically from an instability of the coevolutionary dynamics between genome composition and resource allocation for translation, transcription, and rep ... | 2009 | 19116280 |
| acute parvovirus b19 infection frequently causes false-positive results in epstein-barr virus- and herpes simplex virus-specific immunoglobulin m determinations done on the liaison platform. | during an outbreak of parvovirus b19 we collected serum samples from 68 nonpregnant patients in the region of antwerp (belgium). fifty-seven (84%) of the parvovirus b19 immunoglobulin m (igm)-positive sera had a positive result for epstein-barr virus (ebv) igm by liaison testing, 61 (90%) had a positive result for herpes simplex virus (hsv) igm, 20 (29%) samples were positive for cytomegalovirus igm, and 15 (22%) had a positive result for borrelia burgdorferi sensu lato igm. as assay interferenc ... | 2009 | 19116304 |
| spent culture medium from virulent borrelia burgdorferi increases permeability of individually perfused microvessels of rat mesentery. | lyme disease is a common vector-borne disease caused by the spirochete borrelia burgdorferi (bb), which manifests as systemic and targeted tissue inflammation. both in vitro and in vivo studies have shown that bb-induced inflammation is primarily host-mediated, via cytokine or chemokine production that promotes leukocyte adhesion/migration. whether bb produces mediators that can directly alter the vascular permeability in vivo has not been investigated. the objective of the present study was to ... | 2008 | 19116656 |
| reply to letter by volkman commenting on the possible onset of seronegative disease in lyme arthritis. | 2009 | 19116916 | |
| use of tick surveys and serosurveys to evaluate pet dogs as a sentinel species for emerging lyme disease. | to evaluate dogs as a sentinel species for emergence of lyme disease in a region undergoing invasion by ixodes scapularis. | 2009 | 19119948 |
| characterization and in vitro reaction properties of 19 unique hairpin telomeres from the linear plasmids of the lyme disease spirochete. | the genome of the lyme disease pathogen borrelia burgdorferi contains about a dozen linear dna molecules that carry covalently closed hairpin telomeres as a specialized mechanism for dealing with the end-replication problem. the hairpin telomeres are generated from replicative intermediates through a two-step transesterification promoted by the telomere resolvase rest. although the genome of b. burgdorferi has been sequenced, the sequence of most telomeres has remained unknown because of difficu ... | 2009 | 19122193 |
| efficacy of a long-term antibiotic treatment in patients with a chronic tick associated poly-organic syndrome (tapos). | despite a now codified antibiotic treatment for lyme disease, a significant proportion of patients treated according to recommendations complain of persistent signs and symptoms. the pathophysiological mechanisms which underlie this syndrome of post-treatment chronic systemic illness remain unclear. for some physicians post-treatment symptoms indicate a persistent infection requiring prolonged antibiotic therapy. for others, there is no benefit from antimicrobial therapy. the difficulty of asses ... | 2009 | 19124209 |
| deciphering dorin m glycosylation by mass spectrometry. | the soft tick, ornithodoros moubata, is a vector of several bacterial and viral pathogens including borrelia duttoni, a causative agent of relapsing fever and african swine fever virus. previously, a sialic acid-specific lectin dorin m was isolated from its hemolymph. here, we report on the complete characterization of the primary sequence of dorin m. using liquid chromatography coupled to mass spectrometry, we identified three different glycopeptides in the tryptic digest of dorin m. the peptid ... | 2008 | 19136723 |
| trypanosoma cf.varani in an imported ball python (python reginus) from ghana. | peripheral blood from a ball python (python reginus) imported from ghana was cultured in barbour-stoenner-kelly (bsk) medium for borrelia spp. isolation, resulting in the prominent appearance of free, and clusters of, trypanosomes in a variety of morphological forms. the molecular phylogenetic characterization of these cultured trypanosomes using the small subunit rdna indicated that this python was infected with a species closely related to trypanosoma varani wenyon, 1908, originally described ... | 2009 | 19138059 |
| antibody responses to borrelia ir(6) peptide variants and the c6 peptide in swedish patients with erythema migrans. | the aim of this study was to evaluate the antibody responses to different vlse protein ir(6) peptide variants and the synthetic c6 peptide in acute and convalescent (2-3 and 6 months) serum samples from swedish patients with clinical erythema migrans (em). serum samples were prospectively collected from 148 patients with em and compared to serum samples obtained from 200 healthy blood donors. the igg responses to 3 ir(6) peptide variants originating from borrelia burgdorferi (b. burgdorferi) sen ... | 2009 | 19138558 |
| mutational analyses of the bbcrasp-1 protein of borrelia burgdorferi identify residues relevant for the architecture and binding of host complement regulators fhl-1 and factor h. | borrelia burgdorferi exploits multiple strategies to evade host immune responses. one central immune escape mechanism is the inactivation of the host complement attack by acquisition host complement regulators fhl-1 and factor h via complement regulator-acquiring surface proteins (bbcrasps). the bbcrasp-1 protein is the first bacterial factor h/fhl-1-binding protein for which the atomic structure has been solved. previously, 3 regions including the c terminus were identified as putative contact ... | 2009 | 19138559 |
| live borrelia burgdorferi spirochetes elicit inflammatory mediators from human monocytes via the toll-like receptor signaling pathway. | we investigated the mechanisms that lead to the production of proinflammatory mediators by human monocytes when these cells are exposed in vitro to live borrelia burgdorferi spirochetes. we first focused on myeloid differentiation primary response protein 88 (myd88), an adapter molecule that is essential in the toll-like receptor (tlr) pathway. real-time pcr, flow cytometry, and confocal microscopy experiments revealed that myd88 was maximally expressed in thp-1 cells after 24-h stimulation of t ... | 2009 | 19139200 |
| the great lyme debate. | 2007 | 19146213 | |
| parsonage-turner syndrome revealing lyme borreliosis. | parsonage-turner syndrome, also known as acute brachial neuritis or neuralgic amyotrophy, can be caused by various infectious agents. we report on four patients who experienced parsonage-turner syndrome as the first manifestation of lyme disease. the clinical picture was typical, with acute shoulder pain followed rapidly by weakness and wasting of the shoulder girdle muscles. electrophysiological testing showed denervation. a single patient reported erythema chronicum migrans after a tick bite. ... | 2009 | 19147387 |
| tick saliva affects both proliferation and distribution of borrelia burgdorferi spirochetes in mouse organs and increases transmission of spirochetes to ticks. | ixodes ricinus tick saliva-activated transmission of borrelia burgdorferi sensu stricto spirochetes was studied on the c3h/hen mouse model. the influence of the feeding of uninfected nymphs on the proliferation and distribution of intradermally inoculated spirochetes was compared with the effect of co-inoculated saliva or salivary gland extract (sge), respectively. spirochete loads in murine tissues were evaluated using real-time q-pcr. sge induced significantly increased spirochete numbers in t ... | 2009 | 19147403 |