Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| subset selection of high-depth next generation sequencing reads for de novo genome assembly using mapreduce framework. | recent progress in next-generation sequencing technology has afforded several improvements such as ultra-high throughput at low cost, very high read quality, and substantially increased sequencing depth. state-of-the-art high-throughput sequencers, such as the illumina miseq system, can generate ~15 gbp sequencing data per run, with >80% bases above q30 and a sequencing depth of up to several 1000x for small genomes. illumina hiseq 2500 is capable of generating up to 1 tbp per run, with >80% bas ... | 2015 | 26678408 |
| next-generation whole-genome sequencing of eight strains of bacillus cereus, isolated from food. | bacillus cereus can contaminate food and cause emetic and diarrheal foodborne illness. here, we report whole-genome sequences of eight strains of b. cereus, isolated from different food sources. | 2015 | 26679589 |
| proteolytic activities expressed by gastrointestinal pathogens bacillus cereus, listeria monocytogenes and enterococcus faecium in different growth phases. | bacterial proteases are implicated in protein quality control, biofilm formation or might have a direct function in pathogenesis by processing virulence factors or cleaving host factors. in recent years, knowledge of proteases expressed by gram-negative pathogens remarkably increased. however, investigation of proteases from gram-positive bacteria is rather rare, but required for the analysis of pathogenesis-relevant proteases. in this study, we extracted and detected proteases from the gastroin ... | 2015 | 26682199 |
| detection of viable enterotoxin-producing bacillus cereus and analysis of toxigenicity from ready-to-eat foods and infant formula milk powder by multiplex pcr. | bacillus cereus is responsible for several outbreaks of foodborne diseases due to its emetic toxin and enterotoxin. enterotoxins, cytotoxin k (cytk), nonhemolytic enterotoxin (nhe), and hemolysin bl (hbl), have been recorded in several diarrheal cases due to food poisoning from b. cereus. the objective of this study was to develop a rapid and accurate method that combines multiplex pcr with propidium monoazide to selectively detect viable cells of enterotoxin-producing b. cereus in milk powder, ... | 2016 | 26686715 |
| cost-effective-substrates for production of poly-β-hydroxybutyrate by a newly isolated bacillus cereus ps-10. | poly-β-hydroxybutyrate (phb) may serve as one of the imperative substitutes for petroleum derived plastics because of their close functional analogy and biodegradation quality. in the present study, phb producing ability of bacterial isolates was examined on low-cost agro industrial residues. isolate ps-10 from domestic waste landfills, identified as bacillus cereus ps-10 produced and accumulated appreciable amount of phb. bacillus cereus ps-10 was capable of using a wide variety of agro-based r ... | 2015 | 26688964 |
| molecular characterization and biological effects of a c-type lectin-like receptor in large yellow croaker (larimichthys crocea). | the c-type lectin-like receptors (ctlrs) play important roles in innate immunity as one type of pattern recognition receptors. here, we cloned and characterized a c-type lectin-like receptor (lycctlr) from large yellow croaker larimichthys crocea. the full-length cdna of lycctlr is 880 nucleotides long, encoding a protein of 215 amino acids. the deduced lycctlr contains a c-terminal c-type lectin-like domain (ctld), an n-terminal cytoplasmic tail, and a transmembrane region. the ctld of lycctlr ... | 2015 | 26690423 |
| biocatalytic resolution of rac-α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester by immobilized recombinant bacillus cereus esterase. | a new esterase-producing strain (bacillus cereus wzz001) which exhibiting high hydrolytic activity and excellent enantioselectivity on rac-α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester (r, s-1) has been isolated from soil sample by our laboratory. in this study, the stereoselective hydrolysis of (r, s-1) was performed using the recombinant bacillus cereus esterase which expressed in escherichia coli bl21 (de3). under the optimized conditions of ph 8.0, 35 °c, and concentration of substrate 4 ... | 2016 | 26695776 |
| a strategic approach for rapid synthesis of gold and silver nanoparticles by panax ginseng leaves. | the study highlights the synthesis of gold nanoparticles and silver nanoparticles by fresh leaves of panax ginseng, an herbal medicinal plant. the reduction of auric chloride and silver nitrate led to the formation of gold and silver nanoparticles within 3 and 45 min, at 80°c, respectively. the developed methodology was rapid, facile, ecofriendly and the utmost significant is quite economical, which did not require subsequent processing for reduction or stabilization of nanoparticles. the nanopa ... | 2016 | 26698271 |
| comparative elimination of dimethyl disulfide by maifanite and ceramic-packed biotrickling filters and their response to microbial community. | unpleasant odor emissions have traditionally occupied an important role in environmental concern. in this paper, twin biotrickling filters (btfs) packed with different packing materials, seeded with bacillus cereus gigan2, were successfully constructed to purify gaseous dimethyl disulfide (dmds). the maifanite-packed btf showed superior biodegradation capability to the ceramic-packed counterpart in terms of removal efficiency and elimination capacity under similar conditions. at an empty bed res ... | 2016 | 26702514 |
| production of γ-cyclodextrin by bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system. | aqueous two-phase system (atps) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. extractive bioconversion of gamma-cyclodextrin (γ-cd) from soluble starch with cyclodextrin glycosyltransferase (cgtase, ec 2.4.1.19) enzyme derived from bacillus cereus was evaluated using polyethylene glycol (peg)/potassium phosphate based on atps. the optimum condition was attained in the atps constituted of 30.0% (w/w) peg 3000 g/mol and 7. ... | 2016 | 26702953 |
| [preliminary analyses on bacterial diversity and resistance in infection-related skin disorders]. | to explore the bacterial diversity and resistance in infection-related skin disorders. | 2015 | 26704163 |
| screening and characterization of potential bacillus starter cultures for fermenting low-salt soybean paste (doenjang). | the bacterial strains were screened as potential starters for fermenting low-salt doenjang (a korean traditional fermented soybean paste) using korean doenjang based on proteolytic and antipathogenic activities under 6.5-7.5% nacl conditions. phylogenetic analysis based on 16s rrna gene sequences showed that they all belonged to the genus bacillus. proteolytic and antipathogenic activities against escherichia coli, bacillus cereus, staphylococcus aureus, listeria monocytogenes, and aspergillus f ... | 2016 | 26718466 |
| evaluation of anti-bacterial effects of some novel thiazole and imidazole derivatives against some pathogenic bacteria. | bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bacterial compounds such as some thiazole and imidazole derivatives. thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on bacillus cereus, listeria monocytogenes, escherichia coli, salmonella typhimurium, proteus mirabilis and shigella dysenteriae. | 2015 | 26719785 |
| effects of potential probiotic bacillus cereus en25 on growth, immunity and disease resistance of juvenile sea cucumber apostichopus japonicus. | this study was conducted to determine effects of potential probiotic bacillus cereus en25 (isolated from mud of sea cucumber culturing water bodies) on growth, immunity and disease resistance against vibrio splendidus infection in juvenile sea cucumbers apostichopus japonicus. animals were respectively fed diets with b. cereus en25 at 0 (control), 10(5), 10(7) and 10(9) cfu/g for 30 days. results showed that dietary b. cereus en25 had no significant effects on growth, total coelomocytes counts a ... | 2016 | 26723266 |
| antibacterial effects of alchornea cordifolia (schumach. and thonn.) müll. arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. | the leaves, stems and roots of alchornea cordifolia (schumach. and thonn.) müll. arg. are used as traditional medicine in many african countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds. | 2016 | 26724423 |
| synthesis and properties of cellulose-functionalized poss-sio2/tio2 hybrid composites. | the mechanical, thermal, optical, electrical and morphological properties of cellulose, an excellent natural biomaterial, can be improved by organic-inorganic hybrid composite methods. based on the pristine properties of cellulose, the preparation of cellulose-metal oxide hybrid nanocomposites using a dispersion process of nanoparticles into the cellulose host matrix by traditional methods, has limitations. recently, the functionalized cellulose-polymer-based materials were considered to be an i ... | 2015 | 26726461 |
| microbiological analysis of rice cake processing in korea. | this study was conducted to evaluate the microbial contamination in rice cake materials and products during processing and in the operation environment in nonhazard analysis [and] critical control point factories. furthermore, the environmental health of the processing facilities and the bacterial and fungal contamination on the workers' hands were investigated. pour plate methods were used for enumeration of aerobic plate count (apc), yeast and molds (ym), bacillus cereus, staphylococcus aureus ... | 2016 | 26735044 |
| safety of street-vended soy wara in nigeria. | soy wara is a common ready-to-eat food whose production and sale are currently unregulated. microbiological sampling indicated that 21% of the samples had standard plate counts exceeding 100,000 cfu/g, and 14% had staphylococcus aureus counts higher than 100,000 cfu/g. the occurrence of s. aureus at these levels can result in food poisoning. listeria monocytogenes was isolated in 14.4% of the samples, although the counts were generally low, typically <1,000 cfu/g. although counts of l. monocytog ... | 2016 | 26735046 |
| transcriptome analysis of bacillus thuringiensis spore life, germination and cell outgrowth in a vegetable-based food model. | toxigenic species belonging to bacillus cereus sensu lato, including bacillus thuringiensis, cause foodborne outbreaks thanks to their capacity to survive as spores and to grow in food matrixes. the goal of this work was to assess by means of a genome-wide transcriptional assay, in the food isolate b. thuringiensis uc10070, the gene expression behind the process of spore germination and consequent outgrowth in a vegetable-based food model. scanning electron microscopy and energy dispersive x-ray ... | 2016 | 26742618 |
| structural basis for latency and function of immune inhibitor a metallopeptidase, a modulator of the bacillus anthracis secretome. | immune inhibitor a(inha)-type metallopeptidases are potential virulence factors secreted by members of the bacillus cereus group. two paralogs from anthrax-causing bacillus anthracis (bainha1 and bainha2) were shown to degrade host tissue proteins with broad substrate specificity. analysis of their activation mechanism and the crystal structure of a zymogenic bainha2 variant revealed a ∼750-residue four-domain structure featuring a pro-peptide, a catalytic domain, a domain reminiscent of viral e ... | 2016 | 26745529 |
| advanced molecular diagnostic techniques for detection of food-borne pathogens; current applications and future challenges. | the elimination of disease-causing microbes from the food supply is a primary goal and this review deals with the overall techniques availavle for detection of food-borne pathogens. now-a-days conventional methods are replaced by advanced methods like biosensors, nucleic acid-based tests (nat) and different pcr based techniques used in molecular biology to identify specific pathogens. bacillus cereus, staphylococcus aureus, proteus vulgaris, escherichia coli, campylobacter, listeria monocytogene ... | 2016 | 26745757 |
| antimicrobial activity of neo-clerodane diterpenoids isolated from lamiaceae species against pathogenic and food spoilage microorganisms. | antimicrobial activity of nineteen neo-clerodane diterpenoids, isolated from the acetone extracts of the aerial parts of scutellaria and salvia species (lamiaceae) were tested against thirteen strains belonging to nine different species of pathogenic and food spoilage bacteria aeromonas hydrophila, bacillus cereus, escherichia coli, listeria monocytogenes, proteus vulgaris, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella abony and staphylococcus aureus as well as against two yeast st ... | 2015 | 26749799 |
| rapid detection of cronobacter sakazakii by real-time pcr based on the cgca gene and taqman probe with internal amplification control. | cronobacter sakazakii is a severe virulent strain that is frequently detected in powdered infant formula (pif). therefore, it is necessary to develop a fast and specific detection method. the specificity of our newly developed quantitative real-time pcr (qrt-pcr) was validated with dna from 46 strains. among them, 12 c. sakazakii strains were correctly amplified, whereas no positive florescent signal was observed from 34 nontarget controls. the detection limit of c. sakazakii was about 110 cfu/m ... | 2016 | 26751178 |
| simultaneous detection of listeria monocytogenes, escherichia coli o157:h7, bacillus cereus, salmonella spp., and staphylococcus aureus in low-fatted milk by multiplex pcr. | a rapid and specific pcr assay for the simultaneous detection of listeria monocytogenes, escherichia coli o157:h7, bacillus cereus, salmonella spp., and staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. multiplex pcr developed in this study produced only acta, flic, hbl, inva, iles amplicons, but did not produce any non-specific amplicon. the primer sets successfully amplified the target genes in the multiplex pcr without any non-specific or a ... | 2014 | 26761507 |
| antimicrobial effect of nisin against bacillus cereus in beef jerky during storage. | the microbial distribution of raw materials and beef jerky, and the effect of nisin on the growth of bacillus cereus inoculated in beef jerky during storage, were studied. five strains of pathogenic b. cereus were detected in beef jerky, and identified with 99.8% agreement using api chb 50 kit. to evaluate the effect of nisin, beef jerky was inoculated with approximately 3 log cfu/g of b. cereus mixed culture and nisin (100 iu/g and 500 iu/g). during the storage of beef jerky without nisin, the ... | 2015 | 26761838 |
| microbiology of corneal wounds after cataract surgery: biofilm formation and antibiotic resistance patterns. | to evaluate the bacterial flora of corneal wounds at the end of cataract surgery before intracameral antibiotic use and to determine agents to treat postoperative endophthalmitis, the potential for biofilm formation, and antibiotic resistance. | 2016 | 26762493 |
| butanol production under microaerobic conditions with a symbiotic system of clostridium acetobutylicum and bacillus cereus. | one major problem of abe (acetone, butanol and ethanol) fermentation is high oxygen sensitivity of clostridium acetobutylicum. currently, no single strain has been isolated or genetically engineered to produce butanol effectively under aerobic conditions. in our previous work, a symbiotic system tsh06 has been developed successfully by our group, and two strains, c. acetobutylicum tsh1 and bacillus cereus tsh2, were isolated from tsh06. | 2016 | 26762531 |
| adaptive responses of bacillus cereus atcc14579 cells upon exposure to acid conditions involve atpase activity to maintain their internal ph. | 2015 | 26769448 | |
| genomic characterization and comparison of seven myoviridae bacteriophage infecting bacillus thuringiensis. | bacillus thuringiensis kurstaki, a bacterium that is a source of biopesticides and a safe simulant for pathogenic bacillus species, was used to isolate seven unique bacteriophages. the phage genomes were sequenced and ranged in size from 158,100 to 163,019 bp encoding 290-299 genes, and the gc content of ~38% was similar to that of the host bacterium. all phages had terminal repeats 2-3 kb long. three of the phages encoded trnas and three contained a self-splicing intron in the dna polymerase ge ... | 2016 | 26773385 |
| antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens. | the marine environment is a proven source of structurally complex and biologically active compounds. in this study, the antimicrobial effects of a small collection of marine-derived extracts and isolates, were evaluated against 5 foodborne pathogens using a broth dilution assay. results demonstrated that algal extracts from padina and ulva species and cyanobacterial compounds antillatoxin b, laxaphycins a, b and b3, isomalyngamide a, and malyngamides c, i and j showed antimicrobial activity agai ... | 2016 | 26775951 |
| cody regulates the activity of the virulence quorum sensor plcr by controlling the import of the signaling peptide papr in bacillus thuringiensis. | in gram-positive bacteria, cell-cell communication mainly relies on cytoplasmic sensors of the rnpp family. activity of these regulators depends on their binding to secreted signaling peptides that are imported into the cell. these quorum sensing regulators control important biological functions in bacteria of the bacillus cereus group, such as virulence and necrotrophism. the rnpp quorum sensor plcr, in complex with its cognate signaling peptide papr, is the main regulator of virulence in b. ce ... | 2015 | 26779156 |
| isolation, purification and characterization of antimicrobial protein from seedlings of bauhinia purpurea l. | a novel antimicrobial protein was purified from the seedlings of bauhinia purpurea by sequential procedures entailing ammonium sulfate precipitation, cation exchange chromatography, preparative native-page and a yield of 2.7% was obtained from the crude extract. the purified antimicrobial protein appeared as a single protein band on sds-page with the molecular mass of 20.9 kda. purified antimicrobial protein exhibited a potent antimicrobial activity against both gram-positive and gram-negative b ... | 2016 | 26780468 |
| bacteriostatic mode of action of trypsin-hydrolyzed palm kernel expeller peptide against bacillus cereus. | palm kernel expeller (pke), the by-product derived from the palm kernel oil milling industry, is commonly added to ruminant feed as a source of protein. recent research has demonstrated that the enzymatically hydrolyzed protein is inhibitory to spore-forming bacteria including bacillus cereus. the trypsin-hydrolyzed pke peptide appears to disrupt the membrane integrity and inhibit the intracellular macromolecule metabolism of b. cereus. the addition of the pke peptide (350 and 700 μg/ml) to b. c ... | 2012 | 26781737 |
| comparison of the potency of the lipid ii targeting antimicrobials nisin, lacticin 3147 and vancomycin against gram-positive bacteria. | while nisin (lantibiotic), lacticin 3147 (lantibiotic) and vancomycin (glycopeptides) are among the best studied lipid ii-binding antimicrobials, their relative activities have never been compared. nisin and lacticin 3147 have been employed/investigated primarily as food preservatives, although they do have potential in terms of veterinary and clinical applications. vancomycin is used exclusively in clinical therapy. we reveal a higher potency for lacticin 3147 (mic 0.95-3.8 μg/ml) and vancomyci ... | 2012 | 26781852 |
| fluorescence-based comparative evaluation of bactericidal potency and food application potential of anti-listerial bacteriocin produced by lactic acid bacteria isolated from indigenous samples. | the aim of the present study was to ascertain the potency of anti-listerial bacteriocin produced by lactic acid bacteria (lab) isolated from indigenous samples of dahi, dried fish, and salt-fermented cucumber. a total of 231 lab isolates were obtained from the samples, of which 51 isolates displayed anti-listerial activity. the anti-listerial lab were identified by pcr as lactobacillus sp., pediococcus sp., enterococcus sp., and lactococcus sp. pcr also enabled the detection of class iia bacteri ... | 2012 | 26781854 |
| probiotic activity of a bacterial strain isolated from ancient permafrost against salmonella infection in mice. | bacillus cereus strain f, collected from relict permafrost located in siberia, was analyzed for probiotic activity in the mouse salmonella enterica model. viable bacterial cells were found in frozen soils taken at mammoth mountain in yakutia from a depth below the level of seasonal thawing. geological data indicated the absence of a thawing within millions of years of deposited soils, which helped to ensure the ancient origin of our sample. according to dna analysis, bacterial cells collected fr ... | 2012 | 26782040 |
| comparative activities of cattle and swine platelet microbicidal proteins. | the bactericidal activities of cattle and swine platelet microbicidal proteins (pmps) with their comparison with human pmp were studied. activities of pmp were tested against bacillus subtilis, bacillus cereus, staphylococcus aureus, staphylococcus epidermidis, micrococcus lysodeikticus and escherichia coli. b. subtilis and b. cereus were high susceptible to pmp at very low concentrations. of the gram-positive cocci studied, m. lysodeikticus and s. aureus were the most, and s. epidermidis the le ... | 2009 | 26783170 |
| infective endocarditis due to bacillus cereus in a pregnant female: a case report and literature review. | incidence of infective endocarditis during pregnancy is around 0.006% with high maternal and fetal mortality. bacillus cereus is an extremely rare cause for endocarditis in intravenous drug abusers (ivda) or those with valvular disease or devices such as pacemakers. we report a case of b. cereus endocarditis, which, to the best of our knowledge, has never been reported in pregnancy. a 30-year-old, 25-week pregnant female presented with right shoulder pain, swelling and erythema on the lateral as ... | 2015 | 26793477 |
| α,β-dibromochalcone derivatives--synthesis and antimicrobial activity. | to obtain some chalcones and their dibrominated analogues and to evaluate their antimicrobial potential. | 2016 | 26793867 |
| diversity of sugar acceptor of glycosyltransferase 1 from bacillus cereus and its application for glucoside synthesis. | glycosyltransferase 1 from bacillus cereus (bcgt1) catalyzes the transfer of a glucosyl moiety from uridine diphosphate glucose (udp-glucose) to various acceptors; it was expressed and characterized. the specificity of acceptors was found to be broad: more than 20 compounds classified into o-, s-, and n-linkage glucosides can be prepared with bcgt1 catalysis. based on this work, we conclude that the corresponding acceptors of these compounds must possess the following features: (1) the acceptors ... | 2016 | 26795959 |
| characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food. | a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ... | 2016 | 26803269 |
| the regulation of exosporium-related genes in bacillus thuringiensis. | bacillus anthracis, bacillus cereus, and bacillus thuringiensis (bt) are spore-forming members of the bacillus cereus group. spores of b. cereus group species are encircled by exosporium, which is composed of an external hair-like nap and a paracrystalline basal layer. despite the extensive studies on the structure of the exosporium-related proteins, little is known about the transcription and regulation of exosporium gene expression in the b. cereus group. herein, we studied the regulation of s ... | 2016 | 26805020 |
| evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk. | lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ... | 2016 | 26805974 |
| antimicrobial compounds from endophytic streptomyces sp. bcc72023 isolated from rice (oryza sativa l.). | an endophytic actinomycete strain bcc72023 was isolated from rice (oryza sativa l.) and identified as the genus streptomyces, based on phenotypic, chemotaxonomic and 16s rrna gene sequence analyses. the strain showed 99.80% similarity compared with streptomyces samsunensis m1463(t). chemical investigation led to the isolation of three macrolides, efomycins m (1), g (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-o-methylabierixin (5). to our knowledge, this is the firs ... | 2016 | 26809052 |
| characterization and exposure assessment of emetic bacillus cereus and cereulide production in food products on the dutch market. | the emetic toxin cereulide, which can be produced by bacillus cereus, can be the cause of food poisoning upon ingestion by the consumer. the toxin causes vomiting and is mainly produced in farinaceous food products. this article includes the prevalence of b. cereus and of cereulide in food products in the netherlands, a characterization of b. cereus isolates obtained, cereulide production conditions, and a comparison of consumer exposure estimates with those of a previous exposure assessment. fo ... | 2016 | 26818983 |
| in vitro antibacterial activity of selected medicinal plants traditionally used in vietnam against human pathogenic bacteria. | medicinal plants are widely used for the treatment of different infectious diseases. infectious diseases caused by bacteria have a large impact on public health. this study aimed to determine the in vitro antibacterial activity of the medicinal plants traditionally used in vietnam against the bacterial strains associated with infectious diseases. | 2016 | 26819218 |
| antibacterial and synergistic activity of pentacyclic triterpenoids isolated from alstonia scholaris. | (1) background: alstonia scholaris (apocynaceae) is an important medicinal plant that has been historically used in "dai" ethnopharmacy to treat infectious diseases in china. although various pharmacological activities have been reported, the antimicrobial constitutes of a. scholaris have not yet been identified. the objective of this study is to evaluate the antibacterial constitutes from the leaf extract of a. scholaris and to assess the synergistic effects of isolated compounds with antibioti ... | 2016 | 26821000 |
| isolation and characterization of spore-forming bacilli (sfb) from shepherd's purse (capsella bursa-pastoris). | shepherd's purse (capsella bursa-pastoris), native to europe, is commonly consumed fresh and sometimes inadequately washed before consumption in korea. the objective of this study was to characterize isolates of spore-forming bacilli (sfb) in samples of fresh shepherd's purse. three genera were identified: bacillus (9 species), paenibacillus (3 species), and brevibacillus (1 species). none of the genes of the hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) complexes, or of the emetic toxin ... | 2016 | 26822957 |
| ethanolic extract of nigella sativa protects fe(ii) induced lipid peroxidation in rat's brain, kidney and liver homogenates. | the study describes the effect of ethanolic extract of nigella sativa against fe(ii) induced lipid peroxidation. basal and fe(ii) induced thiobarbituric acid reactive species (tbars) production was significantly inhibited by the ethanolic extract of nigella sativa at 25-200 μg/ml. our data revealed that the extract has high dpph radical scavenging activity at highest tested concentrations. the extract significantly chelated fe(ii) and scavenged hydroxyl (oh) radical at 25-200μg/ml concentration. ... | 2016 | 26826815 |
| antagonistics against pathogenic bacillus cereus in milk fermentation by lactobacillus plantarum zdy2013 and its anti-adhesion effect on caco-2 cells against pathogens. | lactobacillus plantarum zdy2013 is a potential probiotic isolated from fermented bean acid. in this study, we aimed to evaluate the in vitro antimicrobial activity of this organism against bacillus cereus in milk fermentation, the antiadhesion ability on intestinal epithelial cells, as well as its ability to abrogate the cytotoxic effect and expression levels of genes. we found no antimicrobial activity produced by l. plantarum once the ph was adjusted to 6.0 and 7.0. the ph decreased continuous ... | 2016 | 26830743 |
| detection and quantification of viable bacillus cereus group species in milk by propidium monoazide quantitative real-time pcr. | the bacillus cereus group includes important spore-forming bacteria that present spoilage capability and may cause foodborne diseases. these microorganisms are traditionally evaluated in food using culturing methods, which can be laborious and time-consuming, and may also fail to detect bacteria in a viable but nonculturable state. the purpose of this study was to develop a quantitative real-time pcr (qpcr) combined with a propidium monoazide (pma) treatment to analyze the contamination of uht m ... | 2016 | 26830746 |
| identification of cdnl, a putative transcriptional regulator involved in repair and outgrowth of heat-damaged bacillus cereus spores. | spores are widely present in the environment and are common contaminants in the food chain, creating a challenge for food industry. nowadays, heat treatments conventionally applied in food processing may become milder to comply with consumer desire for products with higher sensory and nutritional values. consequently subpopulations of spores may emerge that are sublethally damaged rather than inactivated. such spores may germinate, repair damage, and eventually grow out leading to uncontrolled s ... | 2016 | 26849219 |
| effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. | in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ... | 2016 | 26851857 |
| biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. | biosynthesis of agnps by 37 different bacterial soil isolates was done and confirmed through visible spectrophotometry. fifteen isolates were identified and two of them with the highest ability of agnps production were used for transmission electron microscopy (tem) and x-ray diffraction (xrd) tests. mtt assay for both of the obtained agnps was run and after determination their ic50s, two different toxic and nontoxic doses of each agnps solution were chosen for wound healing assay. forty eight r ... | 2016 | 26854241 |
| understanding the antimicrobial activity behind thin- and thick-rolled copper plates. | the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ... | 2016 | 26860943 |
| antioxidant and dna damage protecting activity of exopolysaccharides from the endophytic bacterium bacillus cereus sz1. | an endophytic bacterium was isolated from the chinese medicinal plant artemisia annua l. the phylogenetic and physiological characterization indicated that the isolate, strain sz-1, was bacillus cereus. the endophyte could produce an exopolysaccharide (eps) at 46 mg/l. the 1,1-diphenyl-2-picrylhydracyl (dpph) radical scavenging activity of the eps reached more than 50% at 3-5 mg/ml. the eps was also effective in scavenging superoxide radical in a concentration dependent fashion with an ec50 valu ... | 2016 | 26861269 |
| uptake and levels of the antibiotic berberine in individual dormant and germinating clostridium difficile and bacillus cereus spores as measured by laser tweezers raman spectroscopy. | spores of clostridium difficile and bacillus cereus are major causes of nosocomial diarrhoea and foodborne disease. our aim was to measure the dynamics of the uptake of the antibiotic berberine by individual germinating spores and the levels of berberine accumulated in germinated spores. | 2016 | 26861569 |
| the pulsed light inactivation of veterinary relevant microbial biofilms and the use of a rtpcr assay to detect parasite species within biofilm structures. | the presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. this study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means ... | 2016 | 26862516 |
| cytotoxic potential of bacillus cereus strains atcc 11778 and 14579 against human lung epithelial cells under microaerobic growth conditions. | bacillus cereus, a food poisoning bacterium closely related to bacillus anthracis, secretes a multitude of virulence factors including enterotoxins, hemolysins, and phospholipases. however, the majority of the in vitro experiments evaluating the cytotoxic potential of b. cereus were carried out in the conditions of aeration, and the impact of the oxygen limitation in conditions encountered by the microbe in natural environment such as gastrointestinal tract remains poorly understood. this resear ... | 2016 | 26870026 |
| investigation of equilibrium and kinetics of cr(vi) adsorption by dried bacillus cereus using response surface methodology. | in this study, response surface methodology (rsm) based on three-variable-five-level central composite rotatable design was used to analyze the effects of combined and individual operating parameters (biomass dose, initial concentration of cr(vi) and ph) on the cr(vi) adsorption capacity of dried bacillus cereus. a quadratic polynomial equation was obtained to predict the adsorbed cr(vi) amount. analysis of variance showed that the effect of biomass dose was the key factor in the removal of cr(v ... | 2016 | 26877045 |
| in vitro antimicrobial activity of spices and medicinal herbs against selected microbes associated with juices. | in the present investigation, comparison of antimicrobial activities of different spices, curcuma longa, zingiber officinale, and mentha arvensis, and medicinal herbs, such as withania somnifera, rauvolfia serpentina, emblica officinalis, terminalia arjuna, and centella asiatica, was evaluated. different extraction solvents (acetone, methanol, ethanol, and water) were used and extracts were examined against bacillus cereus, serratia sp., rhodotorula mucilaginosa, aspergillus flavus, and penicill ... | 2016 | 26880927 |
| association of genotyping of bacillus cereus with clinical features of post-traumatic endophthalmitis. | bacillus cereus is the second most frequent cause of post-traumatic bacterial endophthalmitis. although genotyping of b. cereus associated with gastrointestinal infections has been reported, little is known about the b. cereus clinical isolates associated with post-traumatic endophthalmitis. this is largely due to the limited number of clinical strains available isolated from infected tissues of patients with post-traumatic endophthalmitis. in this study, we report successful isolation of twenty ... | 2016 | 26886446 |
| bacteria-based polythene degradation products: gc-ms analysis and toxicity testing. | polythene degradation leads to the production of various by-products depending upon the type of degradation process. the polythene degradation products (pedp) in the culture supernatant of the two bacteria (lysinibacillus fusiformis strain vasb14/wl and bacillus cereus strain vasb1/ts) were analyzed with gc-ms technique. the major by-products in the pedp in the culture supernatant of l. fusiformis strain vasb14/wl (1,2,3,4 tetra methyl benzene) and b. cereus strain vasb1/ts (1,2,3 trimethyl benz ... | 2016 | 26888528 |
| bacillus licheniformis trehalose-6-phosphate hydrolase structures suggest keys to substrate specificity. | trehalose-6-phosphate hydrolase (trea) belongs to glycoside hydrolase family 13 (gh13) and catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to yield glucose and glucose 6-phosphate. the products of this reaction can be further metabolized by the energy-generating glycolytic pathway. here, crystal structures of bacillus licheniformis trea (bltrea) and its r201q mutant complexed with p-nitrophenyl-α-d-glucopyranoside (r201q-ppng) are presented at 2.0 and 2.05 å resolution, respectively. the ... | 2016 | 26894535 |
| evaluation of a parchment document, the 13th century incorporation charter for the city of krakow, poland, for microbial hazards. | the literature of environmental microbiology broadly discusses issues associated with microbial hazards in archives, but these publications are mainly devoted to paper documents. there are few articles on historical parchment documents, which used to be very important for the development of literature and the art of writing. these studies present a broad spectrum of methods for the assessment of biodeterioration hazards of the parchment document in question. they are based on both conventional m ... | 2016 | 26896133 |
| fabrication of an electrochemical dna-based biosensor for bacillus cereus detection in milk and infant formula. | this paper describes fabrication of a dna-based au-nanoparticle modified pencil graphite electrode (pge) biosensor for detection of bacillus cereus, causative agent of two types of food-borne disease, i.e., emetic and diarrheal syndrome. the sensing element of the biosensor was comprised of gold nanoparticles (gnps) self-assembled with single-stranded dna (ssdna) of nhea gene immobilized with thiol linker on the gnps modified pge. the size, shape and dispersion of the gnps were characterized by ... | 2016 | 26896793 |
| surface modification of a polyethylene film for anticoagulant and anti-microbial catheter. | a functional anticoagulant and anti-bacterial coating for polyethylene (pe) films is described. the stepwise preparation of this nanocomposite surface coating involves o2 plasma etching of pe film, carbodiimide coupling of cysteamine to the etched pe film, binding of ag to sulfhydryl groups of cysteamine, and assembly of heparin capped agnps on the pe film. the nanocomposite film and its components were characterized by (1)h-nuclear magnetic resonance spectroscopy, attenuated total reflectance-f ... | 2016 | 26900340 |
| a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces. | a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ... | 2016 | 26902211 |
| use of peripheral parenteral nutrition solutions as a risk factor for bacillus cereus peripheral venous catheter-associated bloodstream infection at a japanese tertiary care hospital: a case-control study. | the risk factors are unclear for peripheral venous catheter-associated bloodstream infections (pvcbsis) caused by bacillus cereus. we aimed to examine for these risk factors in patients with b. cereus pvcbsi by conducting a 2-year case-control study in a large teaching hospital. we analyzed all adult cases of b. cereus pvcbsi (37 patients) and 180 controls who were randomly selected from among patients who had a pvc in place for at least 2 days. multivariate analysis using a conditional logistic ... | 2016 | 26902219 |
| the influence of interfering substances on the antimicrobial activity of selected quaternary ammonium compounds. | standard cleaning processes may not remove all the soiling typically found in food industry, such as carbohydrates, fats, or proteins. contaminants have a high impact in disinfection as their presence may reduce the activity of disinfectants. the influence of alginic acid, bovine serum albumin, yeast extract, and humic acids was assessed on the antimicrobial activities of benzalkonium chloride and cetyltrimethyl ammonium bromide against bacillus cereus vegetative cells and pseudomonas fluorescen ... | 2013 | 26904590 |
| microbes associated with freshly prepared juices of citrus and carrots. | fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. they contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. in the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot) by serial dilution agar plate technique. a total of 30 juice s ... | 2014 | 26904628 |
| antibacterial effects of cissus welwitschii and triumfetta welwitschii extracts against escherichia coli and bacillus cereus. | antibiotic resistance has increased sharply, while the pace for the development of new antimicrobials has slowed down. plants provide an alternative source for new drugs. this study aimed to screen extracts from cissus welwitschii and triumfetta welwitschii for antibacterial activity against escherichia coli and bacillus cereus. the tests conducted included a susceptibility determination test, analysis of the effect of t. welwitschii on cell wall integrity, and transport across the membrane. it ... | 2015 | 26904744 |
| occurrence and antibacterial susceptibility pattern of bacterial pathogens isolated from diarrheal patients in pakistan. | to determine the occurrence of bacterial pathogens responsible for diarrhea and to engender information regarding the effectiveness of commonly used antibiotic against diarrhea. | 2016 | 26905349 |
| influence of season and type of restaurants on sashimi microbiota. | in recent years, an increase in the consumption of japanese food in european countries has been verified, including in portugal. these specialities made with raw fish, typical japanese meals, have been prepared in typical and on non-typical restaurants, and represent a challenge to risk analysis on haccp plans. the aim of this study was to evaluate the influence of the type of restaurant, season and type of fish used on sashimi microbiota. | 2016 | 26911490 |
| an in silico approach to understand the structure-function properties of a serine protease (bacifrinase) from bacillus cereus and experimental evidence to support the interaction of bacifrinase with fibrinogen and thrombin. | microbial fibrinogenolytic serine proteases find therapeutic applications in the treatment of thrombosis- and hyperfibrinogenemia-associated disorders. however, analysis of structure-function properties of an enzyme is utmost important before its commercial application. in this study, an attempt has been made to understand the structure of a fibrinogenolytic protease enzyme, "bacifrinase" from bacillus cereus strain ab01. from the molecular dynamics trajectory analysis, the modelled three-dimens ... | 2017 | 26919276 |
| crystal structure of the bacillus-conserved mazg protein, a nucleotide pyrophosphohydrolase. | ba1544 from bacillus anthracis was previously annotated as a transcription factor for the gene cluster ba1554 - ba1558, but has not been experimentally characterized. b. anthracis is an obligate pathogen causing fatal inhalational anthrax, and ba1544 is absolutely conserved in bacillus species, including bacillus cereus, bacillus thuringiensis and bacillus mycoides, with 100% sequence identity. to address the function of ba1544, we performed structural and biochemical studies, which revealed tha ... | 2016 | 26920050 |
| glycosylation of bcla glycoprotein from bacillus cereus and bacillus anthracis exosporium is domain-specific. | the spores of the bacillus cereus group (b. cereus, bacillus anthracis, and bacillus thuringiensis) are surrounded by a paracrystalline flexible yet resistant layer called exosporium that plays a major role in spore adhesion and virulence. the major constituent of its hairlike surface, the trimerized glycoprotein bcla, is attached to the basal layer through an n-terminal domain. it is then followed by a repetitive collagen-like neck bearing a globular head (c-terminal domain) that promotes glyco ... | 2016 | 26921321 |
| isolation and structural elucidation of brevibacillin, an antimicrobial lipopeptide from brevibacillus laterosporus that combats drug-resistant gram-positive bacteria. | a new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant staphylococcus aureus, vancomycin-resistant strains of enterococcus faecalis and lactobacillus plantarum, and other gram-positive bacteria. the producer strain, designated osy-i1, was determined to be brevibacillus laterosporusvia morphological, biochemical, and genetic analyses. the antimicrobial agent was extracted from cells of osy-i1 with isopropanol, purified by high-performance ... | 2016 | 26921428 |
| eco-friendly and facile integrated biological-cum-photo assisted electrooxidation process for degradation of textile wastewater. | the present article reports an integrated treatment method viz biodegradation followed by photo-assisted electrooxidation, as a new approach, for the abatement of textile wastewater. in the first stage of the integrated treatment scheme, the chemical oxygen demand (cod) of the real textile effluent was reduced by a biodegradation process using hydrogels of cellulose-degrading bacillus cereus. the bio-treated effluent was then subjected to the second stage of the integrated scheme viz indirect el ... | 2016 | 26921849 |
| a new benzopyrans derivatives from a mangrove-derived fungus penicillium citrinum from the south china sea. | one new benzopyran derivative (2r(*),4r(*))-3,4-dihydro-5-methoxy-2-methyl-2h-1-benzopyran-4-ol (1), together with five known compounds (2-6), were obtained from the etoac extract of the endophytic fungus penicillium citrinum hl-5126 isolated from the mangrove brguiera sexangula var. rhynchopetala collected in the south china sea. their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. all compounds were evaluated for their antibacterial activities. compoun ... | 2016 | 26930107 |
| complete genome sequence of the cold-active bacteriophage vmy22 from bacillus cereus. | the cold-active bacteriophage vmy22, belonging to the podoviridae family, was isolated from mingyong glacier in china. sequence analysis revealed that the genome is 18,609 bp long, with an overall g + c content of 36.4 mol%, and 25 open reading frames (orfs). the sequence contains 46 potential promoters, 6 transcription terminators, and no trnas. most of the orfs show a high degree of similarity to b103 (nc_004165). two noteworthy findings were made. first, one of the predicted proteins, orf 19, ... | 2016 | 26941234 |
| feces derived allergens of tyrophagus putrescentiae reared on dried dog food and evidence of the strong nutritional interaction between the mite and bacillus cereus producing protease bacillolysins and exo-chitinases. | tyrophagus putrescentiae (schrank, 1781) is an emerging source of allergens in stored products and homes. feces proteases are the major allergens of astigmatid mites (acari: acaridida). in addition, the mites are carriers of microorganisms and microbial adjuvant compounds that stimulate innate signaling pathways. we sought to analyze the mite feces proteome, proteolytic activities, and mite-bacterial interaction in dry dog food (ddf). proteomic methods comprising enzymatic and zymographic analys ... | 2016 | 26941650 |
| the effect of storage temperature and duration on the microbial quality of bulk tank milk. | the dairy industry in ireland is currently undergoing a period of expansion and, as a result, it is anticipated that milk may be stored in bulk tanks on-farm for periods greater than 48 h. the objective of this study was to investigate the effects of storage temperature and duration on microbial quality of bulk tank milk when fresh milk is added to the bulk tank twice daily. bulk tank milk stored at 3 temperatures was sampled at 24-h intervals during storage periods of 0 to 96 h. bulk tank milk ... | 2016 | 26947309 |
| four new carbazole alkaloids from murraya koenigii that display anti-inflammatory and anti-microbial activities. | in our present study, four new, designated as murrayakonine a-d (), along with 18 known carbazole alkaloids were isolated from chcl3 : meoh (1 : 1) crude extracts of the stems and leaves of murraya koenigii (linn.) spreng. the structures of the all isolated compounds were characterized by analysis of hr-esi-ms and nmr (1d and 2d spectroscopy) results, and comparison of their data with the literature data. for the first time, all the isolates were evaluated for their anti-inflammatory activities, ... | 2016 | 26947457 |
| new coumarin derivatives from ferula pseudalliacea with antibacterial activity. | in this study, the antibacterial activity of disesquiterpene coumarin and sesquiterpene coumarins obtained from ferula pseudalliacea roots was evaluated by determination of minimum inhibitory concentration using the broth micro-dilution method against seven pathogenic bacterial strains (staphylococcus aureus atcc 25,923, vancomycin resistant clinical strain of enterococcus faecium, bacillus cereus ptcc 1015, escherichia coli atcc 25,922, pseudomonas aeruginosa ptcc 1430, clinical strain of klebs ... | 2016 | 26953526 |
| ebselen and analogs as inhibitors of bacillus anthracis thioredoxin reductase and bactericidal antibacterials targeting bacillus species, staphylococcus aureus and mycobacterium tuberculosis. | bacillus anthracis is the causative agent of anthrax, a disease associated with a very high mortality rate in its invasive forms. | 2016 | 26971857 |
| application of mathematical models for bacterial inactivation curves using hypericin-based photosensitization. | the aim of this study was to evaluate which of the three mathematical models (weibull, geeraerd and logistic) makes the best approximation for hypericin-based photosensitization bacterial inactivation. | 2016 | 26972682 |
| recombinant thiopeptides containing noncanonical amino acids. | thiopeptides are a subclass of ribosomally synthesized and posttranslationally modified peptides (ripps) with complex molecular architectures and an array of biological activities, including potent antimicrobial activity. here we report the generation of thiopeptides containing noncanonical amino acids (ncaas) by introducing orthogonal amber suppressor aminoacyl-trna synthetase/trna pairs into a thiocillin producer strain of bacillus cereus .we demonstrate that thiopeptide variants containing nc ... | 2016 | 26976568 |
| recombinant broad-range phospholipase c from listeria monocytogenes exhibits optimal activity at acidic ph. | the broad-range phospholipase c (plc) from listeria monocytogenes has been expressed using an intein expression system and characterized. this zinc metalloenzyme, similar to the homologous enzyme from bacillus cereus, targets a wide range of lipid substrates. with monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (km). based on a homology model of the enzyme to the b. cereus protein, several active site resi ... | 2016 | 26976751 |
| development of a double-antibody sandwich elisa for rapid detection of bacillus cereus in food. | bacillus cereus is increasingly recognized as one of the major causes of food poisoning in the industrialized world. in this paper, we describe a sensitive double-antibody sandwich enzyme-linked immunosorbent assay (elisa) that was developed for rapid detection of b. cereus in food to minimize the risk of contamination. the polyclonal antibody (pab) and monoclonal antibodies (mabs) specific to b. cereus were generated from rabbit antiserum and mouse ascites, respectively, using the octanoic acid ... | 2016 | 26976753 |
| assessment of the bacterial contamination of hand air dryer in washrooms. | the present study was carried out, using standard techniques, to identify and count the bacterial contamination of hand air dryers, used in washrooms. bacteria were isolated from the air flow, outlet nozzle of warm air dryers in fifteen air dryers used in these washrooms. bacteria were found to be relatively numerous in the air flows. bacterially contaminated air was found to be emitted whenever a warm air dryer was running, even when not being used for hand drying. our investigation shows that ... | 2016 | 26981009 |
| formation and biopharmaceutical characterization of electrospun pvp mats with propolis and silver nanoparticles for fast releasing wound dressing. | antibacterial, antiviral, antifungal, antioxidant, anti-inflammatory, and anticancer activities of propolis and its ability to stimulate the immune system and promote wound healing make it a proper component for wound dressing materials. silver nanoparticles are recognized to demonstrate strong antiseptic and antimicrobial activity; thus, it also could be considered in the development of products for wound healing. combining propolis and silver nanoparticles can result in improved characteristic ... | 2016 | 26981531 |
| purification and characterization of plantaricin jla-9: a novel bacteriocin against bacillus spp. produced by lactobacillus plantarum jla-9 from suan-tsai, a traditional chinese fermented cabbage. | bacteriocins are ribosomally synthesized peptides with antimicrobial activity produced by numerous bacteria. a novel bacteriocin-producing strain, lactobacillus plantarum jla-9, isolated from suan-tsai, a traditional chinese fermented cabbage, was screened and identified by its physiobiochemical characteristics and 16s rdna sequence analysis. a new bacteriocin, designated plantaricin jla-9, was purified using butanol extraction, gel filtration, and reverse-phase high-performance liquid chromatog ... | 2016 | 26985692 |
| detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, plutella xylostella (linnaeus), from india and its possible role in indoxacarb degradation. | diamondback moth (dbm), plutella xylostella (linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. the insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. the present study aimed to determine the diversity of gut microflora in dbm, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. we screened 11 geographic populations of dbm i ... | 2016 | 26991291 |
| extracellular synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 and their biological applications. | the present study highlights the microbial synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 strain, in an efficient way. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. the spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. the energy dispersive x-ray spectroscopy and elemental ... | 2016 | 26992796 |
| shelf life of donkey milk subjected to different treatment and storage conditions. | the aim of this study was to investigate the effects of different treatment conditions on microbiological indicators of donkey milk hygiene and their evolution during shelf life at 4 and 12°c from 3 to 30d, simulating a farm-scale pasteurization and packing system. four treatment conditions were tested: no treatment (raw milk), pasteurization (65°c × 30 min), high-pressure processing (hpp), and pasteurization plus hpp. the microbiological quality of the raw donkey milk investigated was not optim ... | 2016 | 26995124 |
| the phylogeny of bacillus cereus sensu lato. | the three main species of the bacillus cereus sensu lato, b. cereus, b. thuringiensis, and b. anthracis, were recognized and established by the early 1900 s because they each exhibited distinct phenotypic traits. b. thuringiensis isolates and their parasporal crystal proteins have long been established as a natural pesticide and insect pathogen. b. anthracis, the etiological agent for anthrax, was used by robert koch in the 19th century as a model to develop the germ theory of disease, and b. ce ... | 2016 | 26999390 |
| evaluation of bacterial contamination of dental unit waterlines and use of a newly designed measurement device to assess retraction of a dental chair unit. | dental unit waterline (duwl) output water is delivered through instruments of a dental chair unit (dcu) to irrigate and cool teeth. however, these waterlines can be heavily contaminated with bacteria. | 2016 | 27000421 |
| secreted compounds of the probiotic bacillus clausii strain o/c inhibit the cytotoxic effects induced by clostridium difficile and bacillus cereus toxins. | although the use of probiotics based on bacillus strains to fight off intestinal pathogens and antibiotic-associated diarrhea is widespread, the mechanisms involved in producing their beneficial effects remain unclear. here, we studied the ability of compounds secreted by the probiotic bacillus clausii strain o/c to counteract the cytotoxic effects induced by toxins of two pathogens, clostridium difficile and bacillus cereus, by evaluating eukaryotic cell viability and expression of selected gen ... | 2016 | 27001810 |
| phytochemical analysis, antimicrobial and antioxidant activities of euphorbia golondrina l.c. wheeler (euphorbiaceae juss.): an unexplored medicinal herb reported from cameroon. | this study aimed at determining the phytochemical constituents of euphorbia golondrina l.c. wheeler, an alien invasive medicinal herb that is used for the treatment of gastroenteritis related ailments, diabetes, conjunctivitis, gastritis, enterocolitis, tonsillitis, vaginitis, hemorrhoids, prostatism, warts and painful swellings by the mundani people of the mount bambouto caldera in southwestern cameroon, and to evaluate its in vitro antimicrobial and antioxidant activities. susceptibility testi ... | 2016 | 27006873 |